Growth Promotion Research Articles

Colombian cyanobacteria with cytotoxic activity in cancer cell lines.

In the last decade, cyanobacteria have emerged as significant reservoirs of bioactive molecule for the pharmaceutical, cosmetic, and alimentary industries, given the wide spectrum of new possibilities of different organic pigments, proteins, carbohydrates, lipids, and powerful antioxidant sources for specific and stronger cancer treatments, autoimmune syndromes, obesity, and inflammatory diseases. A bioactivity screening was executed for 12 strains of Colombian cyanobacteria (10 marine and 2 freshwater) representative of the orders present in the LAUN culture collection, performing methanol extracts per sample, which was fractionated by reverse phase HPLC protocol, obtaining 8 fractions for each crude extract. All these crude extracts were tested for antimicrobial activities through disk diffusion methodology, and fractions were tested for cytotoxic activity against cancer cell lines by cell viability detection with MTT. Cyanobacteria’s extracts showed considerable cytotoxic activity for osteosarcoma (MG063) and colon cancer (HCT116) cell lines (61 and 66% of reduced viability compared to untreated controls, respectively) and a surprising cell growth promotion for the control group fibroblast (3T3L1) and brain endothelial (HCMEC) cell lines (121 and 127% growth, respectively), no bioactivity was observed in the antimicrobial tests. These findings underscore the expansive cytotoxic potential of Colombian cyanobacteria against cancer cell lines and, notably, their growth-promoting effects on healthy cell lines. This positions them as promising sources of bioactive compounds for future pharmaceutical developments.

Impact of Inclusion Ashwagandha (Withania somnifera) Root Powder as a Growth Promoter in the Diet of the Nile tilapia (Oreochromis niloticus) Fingerlings

Impact of Inclusion Ashwagandha (Withania somnifera) Root Powder as a Growth Promoter in the Diet of the Nile tilapia (Oreochromis niloticus) Fingerlings

Promotion of tomato growth and resistance against Fusarium infection using some bioagents

Promotion of tomato growth and resistance against Fusarium infection using some bioagents

Synchronously upgrading of hydrogen storage thermodynamic, kinetics and cycling properties of MgH2 via VTiMn catalyst

To effectively enhance the hydrogen storage performance of MgH2, the multimetallic catalyst VTiMn was triumphantly synthesized by mechanical alloying, and the MgH2-10 wt% VTiMn composite was further fabricated. The results revealed that VTiMn significantly improved the thermodynamic, kinetics and cycle properties of MgH2. Compared with pure MgH2, VTiMn promotes its plateau pressure to increase by 0.16 MPa and the initial desorption temperature to decrease by 47 K, showing obvious thermodynamic instability. Meanwhile, the MgH2-VTiMn composite can adsorb 5.5 wt% H2 within 72 s and release within 1200 s at 523 K. The activation energies of hydrogen absorption and desorption are 30.1 and 90.5 kJ/mol, respectively. After 130 cycles at 598 K, 83 % hydrogen capacity retention can still be achieved. Characterisation indicated that the composite formed a core–shell structure with MgH2 coated on the VTiMn surface. Part of the Mn element in VTiMn diffused into the Mg matrix, accompanied by the precipitation of nanoscale α-Mn particles at high temperature. The improved absorption kinetics can be attributed to the accelerated H atoms diffusion by the microcracks in VTiMn and the phase boundaries provided by the nano-precipitated phase, while the desorption kinetics benefits from the promotion of Mg nucleation and growth by α-Mn. Due to the effect of temperature, the rate-determining steps of the hydrogen absorption and desorption process of the composite at 598 K change from H atoms diffusion and Mg nucleation growth to H atoms surface penetration, respectively. These findings will facilitate a more comprehensive understanding of multimetallic catalysts.

Identification and performance of endophytic actinobacteria Ahn65 with functions of growth promotion and disease resistance for rice

Identification and performance of endophytic actinobacteria Ahn65 with functions of growth promotion and disease resistance for rice

Genomic Insights into Pseudomonas protegens E1BL2 from Giant Jala Maize: A Novel Bioresource for Sustainable Agriculture and Efficient Management of Fungal Phytopathogens.

The relationships between plants and bacteria are essential in agroecosystems and bioinoculant development. The leaf endophytic Pseudomonas protegens E1BL2 was previously isolated from giant Jala maize, which is a native Zea mays landrace of Nayarit, Mexico. Using different Mexican maize landraces, this work evaluated the strain's plant growth promotion and biocontrol against eight phytopathogenic fungi in vitro and greenhouse conditions. Also, a plant field trial was conducted on irrigated fields using the hybrid maize Supremo. The grain productivity in this assay increased compared with the control treatment. The genome analysis of P. protegens E1BL2 showed putative genes involved in metabolite synthesis that facilitated its beneficial roles in plant health and environmental adaptation (bdhA, acoR, trpE, speE, potA); siderophores (ptaA, pchC); and extracellular enzymes relevant for PGPB mechanisms (cel3, chi14), protection against oxidative stress (hscA, htpG), nitrogen metabolism (nirD, nit1, hmpA), inductors of plant-induced systemic resistance (ISR) (flaA, flaG, rffA, rfaP), fungal biocontrol (phlD, prtD, prnD, hcnA-1), pest control (vgrG-1, higB-2, aprE, pslA, ppkA), and the establishment of plant-bacteria symbiosis (pgaA, pgaB, pgaC, exbD). Our findings suggest that P. protegens E1BL2 significantly promotes maize growth and offers biocontrol benefits, which highlights its potential as a bioinoculant.

Multifaceted and dual-edged native Trichoderma strains from subabul rhizospheric soil to combat Fusarium wilt disease – a sustainable approach

ABSTRACT Fusarium wilt and gummosis disease is a major concern in Subabul plantations, resulting in quantitative and qualitative wood losses to the paper and pulp industry. Growers currently rely on agrochemicals, which are not environmentally safe. Therefore, developing a sustainable control strategy employing biocontrol agents is necessary. For this, the investigation was performed to identify native Trichoderma strains from Subabul rhizospheric soil having antagonistic, disease control efficiency, and PGPR attributes. Six antagonistic Trichoderma (TR1 to TR6) strains were isolated and their efficacy was tested against Fusarium in-vitro by dual plate technique and volatile metabolites. Among these, Trichoderma strains TR1 and TR2 were found efficient in counteracting the growth of F. equiseti (70–90% inhibition) compared to control. The effectiveness was primarily attributed to the increased production of volatile metabolites, siderophores, and the ability to solubilise the nutrients (P and K). Further, six potent Trichoderma strains were identified as T. asperellum (TR1, TR2, TR3, TR6), and T. hamatum (TR4, TR5) through rDNA ITS gene sequencing and phylogenetic analysis. These isolates were mass-multiplied, formulated using Talc powder followed by bio-priming in rooting media. The application of Trichoderma into rooting media reduced the wilt disease caused by artificial inoculation of F. equiseti. Further, TR1 and TR2 were found promising with conferred protection against wilt, enhanced survival rate (10–15%), and improved growth compared to untreated control. Application of adapted TR1 and TR2 antagonists in rooting media during clonal multiplication has the potential wilt control efficiency, enhanced survival rate, and plant growth promotion in Subabul.

Exploring microalgae and endophyte as biostimulants: antioxidant and anti-inflammatory properties of Cannabis sativa L. sprouts under standard and enrichment conditions

Sprouts from germinated seeds of edible herbaceous plants are richer in bioactive compounds and micronutrients than mature plants. In recent years, the consumption of sprouted seeds in diets and the interest in exploring vegetable sprout's potential beneficial effects on human health has increased. In this work, the five-day-old sprouts of Cannabis sativa L. cultivar “Futura 75” were investigated and characterized. Hemp sprouts were obtained by germination tests under standard and enrichment conditions using the microalgal strain Chlorella sp. C2 and the bacterium Sphingomonas sp. Can_S11. The biochemical properties of hemp sprouts were analyzed for total polyphenol and flavonoid content and antioxidant activities (DPPH, ABTS, FRAP, ORAC, and Fe2+-chelating activity) by in vitro assays. A significant increase in phenolic compounds and greater antioxidant and anti-radical activities were observed for hemp sprouts obtained under enrichment conditions (dry microalgal biomass C2 and the bacterium Can_S11). Additionally, these sprouts and C. sativa seeds demonstrated enhanced protective effects on inflamed A549 cells, effectively mitigating the TNF-α induced changes and reducing the expression of IL-8 and COX-2. Overall, PGP (Plant Growth Promoting) microorganisms and microalgae represent a good strategy to promote the development of hemp sprouts and increase their phytochemical content, and antioxidant and anti-inflammatory activity.

Mannan oligosaccharide as an alternative to infeed antibiotics to improve growth performance of broilers: a meta-analysis

ABSTRACT 1. The purpose of this meta-analysis was to evaluate the effect of mannan oligosaccharide (MOS) as an alternative to antibiotic growth promoters (AGP) on feed intake (FI), body weight gain (BWG), and feed conversion ratio (FCR) of broilers. 2. Data from 75,594 broilers were extracted from 17 articles (19 trials) published between January 2010 and March 2023. The main criteria for the publication selection were as follows, at least three treatments applied (negative control group without MOS or AGP versus MOS or AGP supplementation), presence of performance results, and intra-experimental variation associated with the mean of response (such as standard error). Treatments were classified as control, MOS, or AGP, and adjusted means of treatment were compared. Additionally, the average daily gain (ADG) and average daily feed intake (ADFI) of each type of supplementation were calculated relative (Δ) to the control group (ΔADFI and ΔADG) and expressed as a percentage of the difference. 3. Broilers receiving a diet supplemented with MOS had a 3.7% better BWG and 3% better FCR compared to the control diet (P < 0.001), but these variables were similar to the group receiving AGP supplementation. No significant difference was detected in FI among treatments (P > 0.050). The relationship between ΔADG and ΔADFI was linear for the MOS and AGP-supplemented group (P < 0.050). The ΔADG of broilers fed diets supplemented with MOS or AGP was 6.4% and 4.54% when ΔADFI was zero, respectively. The ΔADG of MOS increased by 0.58% for every 1% of increasing observed in ΔADFI. The corresponding value for the increased ΔADG for the AGP group was 0.69%. 4. The results of this meta-analysis indicated that MOS supplementation is effective in increasing BWG and reducing FCR, similar to broilers fed a diet supplemented with AGP. Therefore, MOS is a safe and sustainable alternative for AGP-free poultry production.

Correlation between Waist Circumference and IGF-1 Levels in an Elderly Population in Bali, Indonesia

Background. Hyperinsulinemia due to insulin resistance is hypothesized to act as a promotor of cancer growth. In addition to the direct effects of hyperinsulinemia on cancer cells, the stimulation of tumor cell growth can also be indirectly mediated through growth factors and receptors such as insulin-like growth factor 1 (IGF-1). Increased cancer risk is also associated with increased adipose tissue, such as in abdominal obesity, due to the higher risk of insulin resistance and hyperinsulinemia. Waist circumference is a parameter that indicates an individual's level of adiposity. In addition, the risk of cancer also increases in the elderly as they age. This study aims to assess the correlation between waist circumference and IGF-1 levels in the elderly population in Bali, Indonesia. Methodology. This study used a cross-sectional analytical design conducted in the Melinggih Village, Gianyar Regency. The study was conducted in September 2023. This study has been approved by the Research Ethics Commission number 2020/UN14.2.2.VII.14/LT/2023. The study population included elderly individuals residing in the Melinggih Village who were willing to participate. Data analysis encompassed descriptive analysis and the Spearman correlation test. Result. A total of 88 subjects participated in the study, consisting of 57 females (64.8%) and 31 males (35.2%). A statistically significant but weak correlation coexists between waist circumference and IGF-1 levels. Conclusion. A weak but statistically significant positive correlation was found between waist circumference and IGF-1 levels in the elderly. However, because of the small sample size, another study with a bigger sample size with enough power to investigate this association needs to be done to validate the results of the current study.

Harnessing nature's defenders: unveiling the potential of microbial consortia for plant defense induction against Alternaria blight in cumin.

Present study was aimed to develop an efficient microbial consortium for combating Alternaria blight disease in cumin. The research involved isolating biocontrol agents against Alternaria burnsii, characterizing their biocontrol and growth promotion traits, and assessing compatibility. A pot experiment was conducted during rabi season of 2022-2023 to evaluate the bioefficacy of four biocontrol agents (1F, 16B, 31B, and 223B) individually and in consortium, focusing on disease severity, plant growth promotion, and defense responses in cumin challenged with A. burnsii. Microbial isolates 1F, 16B, 31B, and 223B significantly inhibited A. burnsii growth in dual plate assays (~ 86%), displaying promising biocontrol and plant growth promotion activities. They were identified as Trichoderma afroharzianum 1F, Aneurinibacillus aneurinilyticus 16B, Pseudomonas lalkuanensis 31B, and Bacillus licheniformis 223B, respectively. The excellent compatibility was observed among all selected biocontrol agents. Cumin plants treated with consortia of 1F + 16B + 31B + 223B showed least percent disease index (32.47%) and highest percent disease control (64.87%). Consortia of biocontrol agents significantly enhanced production of secondary metabolites (total phenol, flavonoids, antioxidant, and tannin) and activation of antioxidant-defense enzymes (POX, PPOX, CAT, SOD, PAL, and TAL) compared to individual biocontrol treatment and infected control. Moreover, consortium treatments effectively reduced electrolyte leakage over the individual biocontrol agent and infected control treatment. The four-microbe consortium significantly enhanced chlorophyll (154%), carotenoid content (88%), plant height (78.77%), dry weight (72.81%), and seed yield (104%) compared to infected control. Based on these findings, this environmentally friendly four-microbe consortium may be recommended for managing Alternaria blight in cumin.

Exploring the Diverse Therapeutic Applications of 1, 3-Thiazine: A Comprehensive Review

Thiazine, a six-membered heterocycle containing nitrogen and sulfur atoms, is of paramount importance due to its diverse biological functions and broad therapeutic effects. The pharmacological attributes of 1,3-thiazine span a wide range of activities, including antileukemic, antimycobacterial, anti-inflammatory, sedative, hypnotic, anti-influenza, antituberculosis, melanogenesis inhibition, BACE1 inhibition (with anti-Alzheimer's potential), growth promotion, neuroprotective, and anticonvulsant properties. Consequently, novel synthetic methodologies and the design of new 1,3-thiazine derivatives are significantly influenced by recent research findings. This comprehensive review explores both in vivo and in vitro preclinical studies on the biomedical and therapeutic applications of 1,3-thiazine, highlighting its extensive medical relevance. It is anticipated that derivatization strategies for 1,3-thiazine will open new avenues for the development of innovative biological agents. This review aims to engage researchers, stimulating the creation of promising new treatments and preventive measures for various diseases.

Insight into the preparation and improved properties of cellulose citrate ester hydrogel slow-release fertilizer

The development of new fertilizers with significant water retention capacity and sustainable nutrient supplementation for arid and semi-arid regions remains an important challenge. In this study, a novel slow-release fertilizer (CCEH-SRF) based on cellulose citrate ester (CCE), which is low-cost and biocompatible, was developed using epoxy chloropropane (ECH) as a cross-linker and urea as a fertilizer. The CCEH-SRF exhibited higher water swelling (21.58 g·g−1) and water retention capacity (20.69 % in 7 days) compared to the neat cellulose hydrogel (CH). Furthermore, the CCEH-SRF demonstrated improved release performance with cumulative slow-release rates of 48.0 % at 2 hours in water, and 5.3 %, 65.9 %, and 66.7 % after 1 day, 26 days, and 35 days respectively in simulated soil column percolation assays, whereas the CH-based SRF had inferior release performance. The nutrient slow-release of CCEH-SRF followed the First-order kinetic model and Fick diffusion process accurately. Additionally, the developed CCEH-SRF showed positive growth promotion effects on maize seedlings, indicating its promising practical applicability. Therefore, this work provides a novel approach for developing environmentally friendly SRFs by utilizing natural cellulose derivatives from readily available agricultural waste in fields such as waste reuse, sustainable agriculture, and horticulture.

Effectiveness Test of Indigenous Plant Growth Promoting Rhizobacteria (PGPR) Isolate on the Growth of Acehnese Patchouli (Pogostemon cablin Benth.) Sidikalang Variety

Plant Growth Promoting Rhizobacteria (PGPR) is a group of bacteria that live saprophytically in the root area (rhizosphere) acting as a plant growth promoter. The purpose of this study was to test the effectiveness of indigenous rhizobacterial isolates from Aceh Besar and Aceh Tengah in supporting the growth of Aceh patchouli Sidikalang variety. This research was conducted at the Laboratory of Seed Science and Technology, Faculty of Agriculture, Syiah Kuala University and patchouli planting was carried out at the Patchouli Innovation Park (Nino Park), Syiah Kuala University, which took place from December 2022 to June 2023. This study used a non-factorial Randomized Group Design (RAK) with 3 replications. The factor studied was the type of rhizobacterial isolate consisting of 15 treatment levels, namely 1 treatment without rhizobacteria, 7 treatments given indigenous rhizobacterial isolates from Lambada, Seulimeum District, Aceh Besar Regency and 7 treatments given rhizobacterial isolates from Paya Tampu village, Rusip Antara District, Central Aceh Regency. The observation parameters studied are of plant height, number of leaves, number of branches, stem diameter, area per leaf blade, root length, root volume and wet biomass weight of plants. The results showed that indigenous rhizobacterial isolates from Aceh Besar and Aceh Tengah effectively increased the average plant height of Sidikalang variety patchouli at 4 and 8 MSA (26%, and 24%), number of leaves (46%, and 58%), number of branches (78% and 50%), area per leaf blade (34%), root length (19%), root volume (60%) and plant wet biomass weight (50.61%).

Chitosan for aquaculture: growth promotion, immune modulation, antimicrobial activity, bio-carrier utility, water quality management, and safety considerations

Chitosan for aquaculture: growth promotion, immune modulation, antimicrobial activity, bio-carrier utility, water quality management, and safety considerations

Targeting the tumour vasculature: from vessel destruction to promotion.

As angiogenesis was recognized as a core hallmark of cancer growth and survival, several strategies have been implemented to target the tumour vasculature. Yet to date, attempts have rarely been so diverse, ranging from vessel growth inhibition and destruction to vessel normalization, reprogramming and vessel growth promotion. Some of these strategies, combined with standard of care, have translated into improved cancer therapies, but their successes are constrained to certain cancer types. This Review provides an overview of these vascular targeting approaches and puts them into context based on our subsequent improved understanding of the tumour vasculature as an integral part of the tumour microenvironment with which it is functionally interlinked. This new knowledge has already led to dual targeting of the vascular and immune cell compartments and sets the scene for future investigations of possible alternative approaches that consider the vascular link with other tumour microenvironment components for improved cancer therapy.

Genomic insights into organic acid production and plant growth promotion by different species of phosphate-solubilizing bacteria.

Bacteria can solubilize phosphorus (P) through the secretion of low-molecular-weight organic acids and acidification. However, the genes involved in the production of these organic acids are poorly understood. The objectives of this study were to verify the calcium phosphate solubilization and the production of low-molecular-weight organic acids by diverse genera of phosphate solubilizing bacterial strains (PSBS); to identify the genes related to the synthesis of the organic acids in the genomes of these strains and; to evaluate growth and nutrient accumulation of maize plants inoculated with PSBS and fertilized with Bayóvar rock phosphate. Genomic DNA was extracted for strain identification and annotation of genes related to the organic acids production. A greenhouse experiment was performed with five strains plus 150mg dm- 3 P2O5 as Bayóvar rock phosphate (BRP) to assess phosphate solubilization contribution to maize growth and nutrition. Paraburkholderia fungorum UFLA 04-21 and Pseudomonas anuradhapurensis UFPI B5-8A solubilized over 60% of Ca phosphate and produced high amounts of citric/maleic and gluconic acids in vitro, respectively. Eleven organic acids were identified in total, although not all strains produced all acids. Besides, enzymes related to the organic acids production were found in all bacterial genomes. Plants inoculated with strains UFPI B5-6 (Enterobacter bugandensis), UFPI B5-8A, and UFLA 03-10 (Paenibacillus peoriae) accumulated more biomass than the plants fertilized with BRP only. Strains UFLA 03-10 and UFPI B5-8A increased the accumulation of most macronutrients, including P. Collectively, the results show that PSBS can increase maize growth and nutrient accumulation based on Bayóvar rock phosphate fertilization.

Inoculation of Paraburkholderia sp. GD17 improves seedling growth and tolerance to Cadmium in Chinese cabbage

This study provided evidence at the first time showing that root inoculation with the plant growth-promoting rhizobacteria Paraburkholderia sp. GD17 improved the growth and tolerance to Cd stress in Chinese cabbage seedlings. Under normal conditions, the shoot fresh and dry weight of GD17-inoculated 30-day-old plants increased by about 29 % and 33 %, and their root fresh and dry weight by 104 % and 67 %, respectively, compared with their non-inoculated partners. The GD17-mediated growth promotion could be attributed to its facilitating influence on plant acquisition of nutrient elements and photosynthetic efficiency, and decreasing abscisic acid production. Under Cd stress, an effective alleviation in Cd-induced growth inhibition was observed in GD17 plants relative to non-inoculated control, suggesting that the root inoculation with GD17 played a systemic protective role. The Cd concentration in plant aerial tissues was comparable between GD17 plants and non-inoculated ones, but it was substantially decreased in GD17 plant roots. In response to Cd, GD17-inoculated plants generally showed a stronger ability to absorb and transport nutrient elements to shoots. The GD17-conferred plant tolerance to Cd was also associated with an increased antioxidative capacity companied by declined oxidative damage, optimal levels of phytohormones, increased flavonoid synthesis as indicated by significantly upregulated expression of related genes and activity of phenylalanine ammonia-lyase. Additionally, root inoculation with GD17 effectively mitigated the Cd-induced decline in photosynthetic efficiency. Collectively, this study firstly showed that GD17-conferred growth-promotion and Cd-tolerance in Chinese cabbage was correlated with multiple regulatory roles in plant metabolism, which, in most cases, was involved in the regulation at the transcription levels of relevant genes.

Implications of Domestication in Theobroma cacao L. Seed-Borne Microbial Endophytes Diversity

The study of plant–microbe interactions is a rapidly growing research field, with increasing attention to the role of seed-borne microbial endophytes in protecting the plant during its development from abiotic and biotic stresses. Recent evidence suggests that seed microbiota is crucial in establishing the plant microbial community, affecting its composition and structure, and influencing plant physiology and ecology. For Theobroma cacao L., the diversity and composition of vertically transmitted microbes have yet to be addressed in detail. We explored the composition and diversity of seed-borne endophytes in cacao pods of commercial genotypes (ICS95, IMC67), recently liberated genotypes from AGROSAVIA (TCS01, TCS19), and landraces from Tumaco (Colombia) (AC9, ROS1, ROS2), to evaluate microbial vertical transmission and establishment in various tissues during plant development. We observed a higher abundance of Pseudomonas and Pantoea genera in the landraces and AGROSAVIA genotypes, while the commercial genotypes presented a higher number of bacteria species but in low abundance. In addition, all the genotypes and plant tissues showed a high percentage of fungi of the genus Penicillium. These results indicate that domestication in cacao has increased bacterial endophyte diversity but has reduced their abundance. We isolated some of these seed-borne endophytes to evaluate their potential as growth promoters and found that Bacillus, Pantoea, and Pseudomonas strains presented high production of indole acetic acid and ACC deaminase activity. Our results suggest that cacao domestication could lead to the loss of essential bacteria for seedling establishment and development. This study improves our understanding of the relationship and interaction between perennial plants and seed-borne microbiota.

Evaluating the Potential of Bacillus Isolates for Chlorpyrifos Degradation and Their Role in Tea Growth Promotion and Suppression of Pathogens.

Pesticides employed for controlling domestic and agricultural pests are among the most dangerous environmental contaminants. Nevertheless, negligent usage and a lack of technical expertise have led to the contamination and pollution of various ecological niches. The extensive utilization of the organophosphate chlorpyrifos (CPs) for insect infestation control, coupled with its detrimental effects and persistence in the ecosystem, has led to calls for its removal from contaminated sites. The study is mainly focused on degradation of CPs; using viz. Bacillus wiedmannii A3 and Bacillus cereus P14 isolated from tea rhizosphere soil having pesticide contamination in Sonitpur district, Assam, India. These two bacterial strains were able to degrade CPs in vitro within 3days. Reverse-phase HPLC analysis suggested about 96% reduction of CPs concentration upon bacterial treatment. Again, in case of A3, GC-MS analysis revealed that CPs was modified to 2-hydroxy-3,5,6-trichloropyridine and chlorpyrifos-oxon, thus finally metabolized into non-toxic products. While analyzing P14, silane, dimethyl (2,2,2-trichloroethoxy) propoxy, and 3-aminobenzoic acid, N-trimethylsilyl-, trimethylsilyl ester were identified. These compounds were subsequently transformed into non-toxic products. In addition to this, they demonstrated a significant boost of plant growth-promoting traits in both absence and presence of CPs; also showed growth development in nursery scale condition. Moreover, they functioned as biocontrol agents against Phellinus lamaensis and Colletotrichum gloeosporioides, responsible for brown root rot and anthracnose in North East India tea plantations, respectively. Thus, the pesticide-tolerant Bacilli strains A3 and P14 could be used as bioremediation of contaminated sites and also as biostimulants, and biocontrols in tea crop production.