ContextEnhancing crop nitrogen absorption is crucial for improving nitrogen fertilizer utilization efficiency. Soil oxygen conditions play a vital role in influencing crop nutrient uptake. However, the impact of dissolved oxygen concentration in irrigation water on cotton’s nitrogen absorption remains unknow under mulched drip irrigation conditions. RESEARCH QUESTION: This study aimed to investigate whether the dissolved oxygen concentration in irrigation water affects cotton’s nitrogen uptake. METHODS: Urea was utilized as the nitrogen fertilizer, and 5 dissolved oxygen concentration of irrigation water were set in the experiment by adding sodium percarbonate (CK: 3.9 mg L−1, CO1: 9.2 mg L−1, CO2: 13.3 mg L−1, CO3: 17.3 mg L−1, CO4: 25.5 mg L−1). RESULTS: The results showed that increasing the dissolved oxygen concentration in irrigation water could increase the soil urease activity and bacterial operational taxonomic units (OTU), which may be beneficial to the urea decomposition and nitrogen cycling function. Towards the end of the cotton growth period, oxygenation irrigation led to an increase in nitrate nitrogen and ammonium nitrogen content in the soil. The average nitrate nitrogen content in the 0–60 cm soil layer of CO1, CO2, CO3 and CO4 increased by 12.4 %, 14.7 %, 15.9 % and 17.3 % respectively compared with CK. Similarly, the average ammonium nitrogen content of CO1, CO2, CO3 and CO4 in the 0–40 cm soil layer increased by 8.9 %, 11.7 %, 12.0 % and 11.6 % respectively compared with CK. Increasing the dissolved oxygen concentration in irrigation water resulted in elevated dry matter accumulation in cotton. The total dry matter of CO1, CO2, CO3 and CO4 increased by 9.5 %, 12.6 %, 14.6 % and 16.8 % respectively compared with CK. Additionally, the nitrogen concentration in each cotton organ also exhibited an increase. The total nitrogen accumulation of CO1, CO2, CO3 and CO4 increased by 26.1 %, 36.2 %, 48.2 % and 53.1 % respectively compared with CK. Furthermore, increasing the dissolved oxygen concentration in irrigation water also facilitated nitrogen migration to cotton bolls (boll shell + seed + fiber). The cotton boll nitrogen accumulation of CO1, CO2, CO3 and CO4 increased by 1.8 %, 3.4 %, 4.2 % and 4.2 % (absolute value) compared with CK (reached significant level). Finally, the lint yield and fiber quality parameters (micronaire, fiber elongation, fiber breaking strength) exhibited further improvement with the increase in dissolved oxygen concentration in irrigation water. CONCLUSIONS: Increasing the dissolved oxygen concentration in irrigation water promotes nitrogen absorption in cotton, facilitates nitrogen accumulation in cotton bolls, and enhances nitrogen fertilizer utilization efficiency. Moreover, it positively impacts cotton lint yield and fiber quality.