Photocatalysis technology has attracted great attention in the field of wastewater purification for plantation purposes. In the present work, we report the preparation, characterization, and application of flexible γ/γ′ -Bi2MoO6/N-doped carbon (Bi2MoO6/N-C) nanofiber membranes in tetracycline (TC) treatment and Vigna radiata growth. The phytotoxicity of degraded intermediates in treated water was evaluated for seed germination and growth of Vigna radiata. The as-prepared flexible Bi2MoO6/N-C homojunction nanofiber membrane, composed of subunits of γ-Bi2MoO6 nanoparticles and γ′ -Bi2MoO6 nanorods, showed strong visible-light harvesting capability, and good photogenerated electron-hole separation, which displayed the better photocatalytic efficacy in the TC treatment, in terms of degradation rate (97%, 60 min), and recyclability (4 cycles). Further, the germination and growth of Vigna radiata seeds were used to check the phytotoxicity level of the photocatalyst itself (0–10,000 mg/L), the treated and untreated TC solution. Phytotoxicity studies were undertaken via growing Vigna radiata in this Bi2MoO6/N-C photocatalyst solution and treated TC solution. The results demonstrated that Vigna radiata thrived in photocatalyst solution even at a high concentration of 10,000 mg/L and TC solution treated by the photocatalyst. Our work could afford an effective multifunctional photocatalyst for improving water quality for irrigation and plantation purposes.