Growth Of Tobacco Research Articles

Silver nanoparticles affect germination and photosynthesis in tobacco seedlings

Extensive commercialization of silver nanoparticles (AgNPs) raises the risk of their accumulation in the soil-plant system. Once released into the environment, AgNPs are prone to chemical transformations, which make it hard to determine whether their phytotoxic effects are purely NP-related or a consequence of released Ag+ ions. In this study the effects of 25, 50, 75, 100 and 150 μM AgNPs and AgNO3 on seed germination and early growth of tobacco (Nicotiana tabacum L.) seedlings were compared. Additionally, the effects on photosynthetic performance and pigment content were investigated. Germination rate and index values indicated delayed and slower germination in some AgNP treatments. Lower AgNP concentrations stimulated root growth, but induced a prominent reduction in fresh weight. By contrast, all AgNO3 concentrations inhibited root growth but only the higher ones decreased fresh weight. Obtained results imply that the observed AgNP toxicity could be ascribed to NP form and can be correlated with high AgNP stability in the solid medium. On the other hand, the majority of AgNP and AgNO3 treatments induced an increase in chlorophyll content that was accompanied by significantly lower values of relative electron transport rate and coefficient of photochemical quenching, implying an inhibition of the electron transport chain. A similar impact of AgNPs and AgNO3 on photosynthesis can be correlated with lower stability of AgNPs in a liquid medium, resulting in AgNP aggregation and dissolution of Ag+ ions.

PENGARUH PUPUK ORGANIK DAN ANORGANIK ZA DAN SP 36 TERHADAP HASIL DAN MUTU TEMBAKAU TEMANGGUNG PADA TANAH ANDISOL

Effect of organic (biogrcen) and inorganic fetilizers (ammonium sulphate and SP36) on the yield and quality of the temanggung tobacco in andosol soil was studied at Galapansari - Temanggung, from Februai to December 2001. The organic and inorganic fetilizers were formulated in 10 packages, namely : (1) 570 kg ammonium sulphate (AS) ♦ 100 kg SP36; (2) the recommended rate for temanggung tobacco (20 000 kg stable manure 500 kg AS + 100 kg SP36; (3) 5 000 kg organic fetilizer (OF); (4) 7 500 kg OF; (5) 8 500 kg OF; (6) 10 000 kg OF; (7) 12 500 kg OF; (8) 14 500 kg OF; (9) 7 500 kg OF + 3 500 kg sUble manure (SM); (10) 7 500 kg OF + 4 500 kg SM. All the rates of fetilizers were based on one hectare planted area (16 500 plants) The expeiment was designed as a randomized block in four replicates. Results showed that organic fertilizer waa more effective than stable manure in improving die growth, yield and quality of temanggung tobacco. A the rate of 5 000 kg was sufficient to produce high ( 6 313 kg fresh or 800 kg sliced died leaves), grade index (51.68) and crop index (55.31). Key words : Organic fetilizer, ammonium sulfate, SP36, temanggung tobacco, andisol

Promoting effect of Nano-Se on tobacco growth and reactive oxygen species metabolism

Se is beneficial to plants’ growth and it is an essential mineral for animals and humans. However, relatively little is known about the relationship between nano-Se concentrations and plant antioxidant activity. In this work, a pot experiment was carried out using two cultivars of tobacco and increasing nano-Se concentrations. The changes of plant biomass, root growth, chlorophyll content, nutrient accumulation and reactive oxygen species (ROS) content in tobacco leaves after being sprayed with nano-Se were studied. The results indicated that the growth of tobacco can be promoted by applying nano-Se in an appropriate concentration. In addition, our research proves that there is cultivars variation in the response of tobacco to increased concentrations of nano-Se. The biomass, root growth characteristics, chlorophyll content and N accumulation of E'yan 1 were increased significantly and were the highest at 5.0 mg L−1 of nano-Se. On the other hand, the above mentioned indicators for K326 reached the maximum value at 2.5 mg L−1 and decreased at 5.0 mg L−1 of nano-Se. The antioxidant enzymes activity were increased by the application of nano-Se, however cultivars variation was detected. The application of nano-Se reduced the amount of thiobarbituric acid reactive substances (TBARS) and ROS at 5.0 mg L−1 for both cultivars. This study confirms that nano-Se affects positively the growth and oxidative status of tobacco plants. However, this study points out that more research is needed to understand the interaction phenomena observed here between tobacco cultivars and concentrations specifically for the anti-oxidant status of the plant.

MhNRAMP1 From Malus hupehensis Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli.

Excessive cadmium (Cd) damages plants by causing cell death. The present study discusses the function of natural resistance-associated macrophage protein (NRAMP) on cell death caused by Cd in Malus hupehensis. MhNRAMP1 was isolated from M. hupehensis roots, and its protein was located in the cell membrane as a transmembrane protein characterized by hydrophobicity. MhNRAMP1 expression in the roots was induced by Cd stress and calcium (Ca) deficiency. MhNRAMP1 overexpression increased Cd concentration in yeasts and enhanced their sensitivity to Cd. Phenotypic comparisons of plants under Cd stress revealed that the growth of transgenic tobacco and apple calli overexpressing MhNRAMP1 was worse than that of the wild type (WT). The Cd2+ influx of transgenic tobacco roots and apple calli was higher, and the recovery time of the Cd2+ influx to a stable state in transgenic apple calli was longer than that of the WT. Cd accumulation and the percentage of apoptotic cells in transgenic lines were higher. Correspondingly, the caspase-1-like and vacuolar processing enzyme (VPE) activities and MdVPEγ expression were higher in transgenic apple calli, but the expression levels of genes that inhibit cell death were lower than those in the WT under Cd stress. Moreover, the Cd translocation from the roots to leaves was increased after MhNRAMP1 overexpression, but the Cd translocation from the leaves to seeds was not affected. These results suggest that MhNRMAP1 exacerbated Cd-induced cell death, which was accomplished by mediating Cd2+ uptake and accumulation, as well as stimulating VPE.

Dipeptides and Glycine Modulate Development of Seedlings and Regenerants of Tobacco Nicotiana tabacum L.

It is revealed that dipeptides glycylglycine (GlyGly) and glycylaspartic acid (GlyAsp), as well as the amino acid glycine (Gly), appreciably stimulated the growth and development of tobacco (Nicotiana tabacum L.) regenerants and seedlings if they were present in a medium at 10–7 М. All three compounds GlyGly, GlyAsp, and Gly influenced the cell differentiation and morphogenic processes in the calli. The compounds modulated expression of the KNOX and GRF family genes. The profiles of induction or repression of gene expression by one and the same peptide were found to differ in tobacco regenerants and seedlings. It is concluded that GlyGly, GlyAsp, and Gly may be considered as regulators of plant growth and development, the mode of action of which is signaling and mainly epigenetic.

The response of flue-cured tobacco cultivar K326 to nitrogen fertilizer rate in China

AbstractNitrogen is an important element for the growth of flue-cured tobacco and is closely related to its yield and quality. In order to pursue higher economic benefits, excessive fertilizer is generally applied in flue-cured tobacco production, which is unfavourable for the sustainable development of flue-cured tobacco production and for the environment. In 2016 and 2017, experiments using different nitrogen fertilizer application rates in flue-cure tobacco were conducted in Yunnan province, and the changes in agronomic, economic and chemical indices as well as in residual soil nitrogen were compared. Linear and quadratic models were used to compare the response of tobacco to nitrogen fertilizer. With increasing nitrogen fertilizer rate, the proportions of superior to medium tobacco and the average price of flue-cured tobacco leaves initially increased and then decreased, while fresh weight, dry weight and the proportion of inferior tobacco showed the opposite trend. Total sugar and reducing sugar contents decreased with increasing nitrogen fertilizer rates, while total nitrogen and nicotine contents increased. Sensory evaluation scores had the highest value when 90 kg N/ha and 120 kg N/ha were applied. Soil nitrate contents increased as nitrogen fertilizer rate increased. The quadratic model was suitable for the response of cultivar K326 to nitrogen and 90 kg N/ha could meet the needs of cultivar K326.

Response of grafting tobacco to low potassium stress

BackgroundIn the previous study, we investigated the alleviation effect of grafting on potassium uptake in roots and tobacco growth inhibition under low potassium stress. However, the effect of grafting on the low potassium stress perception and coping mechanism of tobacco at the whole plant level is not clear now. In order to clearly understand the impact of grafting on potassium deficit responding mechanism in tobacco, a mutual grafting experiment has been conducted in two varieties of tobacco (‘Wufeng No.2’ and ‘Yunyan 87’) in different K supply level (5 mmol L− 1 and 0.5 mmol L− 1 K).ResultsThe results show that compared with the self-rooted seedlings, grafting significantly increased the potassium content of the whole plant of Yunyan 87 (97.57 and 189.74% under normal potassium and low potassium conditions, respectively), and the increase in shoots was greater. The data of whole plant K content distribution and tobacco hypocotyls net K+ flux demonstrates that potassium stress makes plants more inclined to maintain K+ in the shoot rather than root. In addition, when K deficiency occurs, grafting could reduce the time required for downward net K+ flux in tobacco hypocotyl to decrease to stable levels. The results of net K+ flux in the roots indicated that K channel proteins and transporters play different roles in two rootstocks in terms of potassium tolerance. Transcription level analysis suggested that the increased circulating efficiency of K+ between the shoots and roots in tobacco constitutes one means to low potassium stress adaptation.ConclusionsGrafting can activate more K+ channels in tobacco ‘Yunyan 87’, this means a more active K+ cycle, higher potassium content in shoot and faster response to low potassium stress signals in grafting tobacco. In addition, grafting can also change the K+ absorption mode of tobacco root from being dominated by HATS to being jointly responsible by HATS and LATS, greatly improving the ability of K+ transmembrane transportation on root surface under low potassium stress. These are undoubtedly the reasons why grafting tobacco performs better in coping with low potassium stress.

Diving Into Reef Ecosystems for Land-Agriculture Solutions: Coral Microbiota Can Alleviate Salt Stress During Germination and Photosynthesis in Terrestrial Plants.

From their chemical nature to their ecological interactions, coral reef ecosystems have a lot in common with highly productive terrestrial ecosystems. While plants are responsible for primary production in the terrestrial sphere, the photosynthetic endosymbionts of corals are the key producers in reef communities. As in plants, coral microbiota have been suggested to stimulate the growth and physiological performance of the photosynthetic endosymbionts that provide energy sources to the coral. Among them, actinobacteria are some of the most probable candidates. To explore the potential of coral actinobacteria as plant biostimulants, we have analyzed the activity of Salinispora strains isolated from the corals Porites lobata and Porites panamensis, which were identified as Salinispora arenicola by 16S rRNA sequencing. We evaluated the effects of this microorganism on the germination, plant growth, and photosynthetic response of wild tobacco (Nicotiana attenuata) under a saline regime. We identified protective activity of this actinobacteria on seed germination and photosynthetic performance under natural light conditions. Further insights into the possible mechanism showed an endophytic-like symbiosis between N. attenuata roots and S. arenicola and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity by S. arenicola. We discuss these findings in the context of relevant ecological and physiological responses and biotechnological potential. Overall, our results will contribute to the development of novel biotechnologies to cope with plant growth under saline stress. Our study highlights the importance of understanding marine ecological interactions for the development of novel, strategic, and sustainable agricultural solutions.

The study of carbon budget on field-tobacco ecosystem

The carbon balance of the agricultural ecosystem is of great significance for the assessment of the source and sink of the terrestrial ecosystem. In view of the particularity of the growth and management of flue-cured tobacco, the carbon budget of the field-tobacco ecosystem was studied in order to provide the basis for the improvement of tobacco field management and the estimation of agricultural carbon sink. In this study, the carbon budget was observed with single application of chemical fertilizer using the long-term positioning test station for three years from 2015 to 2017. The results showed that the average cumulative biomass of flue-cured tobacco, the average carbon content of tobacco plant, and the cumulative carbon sequestration were (5832.10 ± 537.32) kg hm-2, (42.14 ± 0.05)%, and (2459.25 ± 233.78) kg hm-2, respectively. The ratio of root carbon to plant carbon was higher, with an average of 24.94%. The wet deposition carbon and the dry deposition carbon were 115.32 kg hm-2 and 6.54 kg hm-2 respectively, both accounting for 20.01% of root carbon amount. The total carbon output in growing season was 2464.98 kg hm-2, in which the CO2 emission expenditure accounts for 98.99%, the runoff carbon expenditure 0.76%, and the leaching carbon accounts for 0.25%. The flue-cured tobacco ecosystem was a weak carbon sink to the atmosphere, with a carbon sink of 116.13 kg hm-2. The total amount of carbon sequestration in flue-cured tobacco was relatively lower, whereas its root system had a relatively higher contribution to soil carbon.

Vegetation characteristics in The Soil Geomorphology Perspective

This research lies in the hamlet of the village wells are Klompangan, Ajung, Jember. Research carried out aims to analyze the coordinates of the location, the degree of slope (elevation), soil pH, soil texture, soil moisture, the availability of organic materials, and the color of soil on land relate with tobacco and soil geomorphology aspect. Measurement and research carried out on 3 May 2019 by using descriptive method. Location of site selection has taken 500 meter minimum distance of each point by taking a 5 point location. With research and measurement of physical and chemical aspects of the soil in tobacco fields. From the research and measurement in mind that the average pH in the region ranges from 5.9, with the texture of sandy loam rara average, at an average altitude of 84mdpl, average humidity 9.2. Moreover, this study shows that there is a relationship between tertiary volcanic landforms and tobacco growth as an example of vegetation type.

A Na2CO3-Responsive Chitinase Gene From Leymus chinensis Improve Pathogen Resistance and Saline-Alkali Stress Tolerance in Transgenic Tobacco and Maize.

Salinity and microbial pathogens are the major limiting factors for crop production. Although the manipulation of many genes could improve plant performance under either of these stresses, few genes have reported that could improve both pathogen resistance and saline-alkali stress tolerance. In this study, we identified a new chitinase gene CHITINASE 2 (LcCHI2) that encodes a class II chitinase from Leymus chinensis, which grows naturally on alkaline-sodic soil. Overexpression of LcCHI2 increased chitinase activity in transgenic plants. The transgenic tobacco and maize exhibited improved pathogen resistance and enhanced both neutral salt and alkaline salt stress tolerance. Overexpression of LcCHI2 reduced sodium (Na+) accumulation, malondialdehyde content and relative electrical conductivity in transgenic tobacco under salt stress. In addition, the transgenic tobacco showed diminished lesion against bacterial and fungal pathogen challenge, suggesting an improved disease resistance. Similar improved performance was also observed in LcCHI2-overexpressed maize under both pathogen and salt stresses. It is worth noting that this genetic manipulation does not impair the growth and yield of transgenic tobacco and maize under normal cultivation condition. Apparently, application of LcCHI2 provides a new train of thought for genetically engineering saline-alkali and pathogen resistant crops of both dicots and monocots.

Impact of magnetically treated water on the growth and development of tobacco (Nicotiana tabacum var. Turkish)

Magnetism is one of the physical methods affecting water properties. It is considered as an environmental factor that plays a role in the physiological and biochemical reactions. A hydroponic experiment was conducted using four types of treated water (distilled water, magnetically treated distilled water, magnetically treated tap water, and tap water). Tobacco plants (Nicotiana tabacum var. Turkish) were placed in a growth chamber for three weeks. Plants irrigated with magnetically treated distilled water had a significant increase in the physiological parameters including shoot height and root length (P < 0.0001). The same pattern was seen in the photosynthetic rate and protein content, but no significant differences in the stomatal conductance and transpiration rate (P < 0.5601). In contrast, a significant increase of total carbohydrate content was exhibited in plant irrigated with tap water (P < 0.0064). Electron micrographs showed deformed chloroplasts with damaged thylakoid membranes associated with plastoglobules in plants irrigated withtap water and magnetically treated tapwater. Lastly, this study suggests that magnetically treated water is an excellent option to improve irrigation methods and thus obtains agricultural production with high efficiency.

Hand Hoeing Weeding Frequency on Growth of Tobacco under the Ecological Conditions of Shewa Robit and Bilatte Tobacco Farms, Ethiopia

Hand Hoeing Weeding Frequency on Growth of Tobacco under the Ecological Conditions of Shewa Robit and Bilatte Tobacco Farms, Ethiopia

Research and Application of Biochar in Soil CO2 Emission, Fertility, and Microorganisms: A Sustainable Solution to Solve China’s Agricultural Straw Burning Problem

This study aimed to explore a new way to address the burning of agricultural waste in China while achieving the sustainable use of it. Three agricultural wastes (Wheat straw, peanut shell, and rice husk) were slowly pyrolyzed into biochar, which was subsequently added to the soil to reduce CO2 emissions from the soil, and to improve soil fertility as well as microbial community structure. The biochar and raw materials were added to the soil and cultured under controlled conditions, and then the CO2 emissions produced from the mixing. At the same time, this study used pot experiments to determine the effects of biochar on tobacco soil physical and chemical properties and, therefore, the microbial communities of the soil. This study suggests that (1) biochar can effectively reduce soil CO2 emission rate. Compared with the control, peanut shell biochar could reduce the total CO2 emissions of soil by 33.41%, and the total CO2 emissions of wheat straw biochar treatment was 90.25% lower than that of wheat straw treatment. (2) The soil’s physical and chemical properties were improved. The soil bulk density of wheat straw biochar treatment kept 34.57% lower than that of the control as well as 21.15% lower than that of wheat straw treatment. The soil’s organic carbon of peanut shell biochar treatment was 87.62% more than that of peanut shell treatment. (3) Biochar changed soil microbial community structure. (4) Biochar is suitable for tobacco growth. Peanut husk biochar significantly increased the total biomass of tobacco, and wheat straw biochar significantly increased tobacco root vigor. This study concluded that processing Chinese agricultural waste into biochar and adding it to the soil instead of burning it directly would be an effective means to reduce greenhouse gas emissions, to improve soil, and to promote crop growth.

Phytohormone content variation manipulated by Bemisia tabaci participated in inhibiting tobacco growth: Gibberellin may play a crucial role

Phytohormone content variation manipulated by Bemisia tabaci participated in inhibiting tobacco growth: Gibberellin may play a crucial role

Study on Climate Changes for Flue-Cured Tobacco Growth in Chenzhou City of Hunan Province

Chenzhou city is the most typical region of Nanling Hill Ecological Zone of flue-cured tobacco with of aroma style of burnt sweet and pure sweet in China and is the largest planting region of flue-cured tobacco in Hunan province. It is of significance to study the changes of climatic parameters with time because climate is the most important factor in determining the growth, yield and quality of flue-cured tobacco. In this study, the data of climatic parameters including cumulative sunshine hours(S), mean temperature (T) and cumulative precipitation (P)from 1952 to 2018 in Chenzhou were used to analyze their changes at the scales of year, whole field period and growing period of flue-cured tobacco. The results showed that at the scale of year, the cumulative S, mean T and cumulative P were ranged from 1029.0 h to 2072.1 h, from 17.1°C to 19.5°Cand from 897.1 mm to 2244.6 mm, with the means of 1449.7 h, 18.1°C and 1482.5 mm, respectively. At the scale of whole field period of flue-cured tobacco, the cumulative S, mean T and cumulative P were ranged from 298.1 h to 807.2 h, from 21.2°C to 23.9°C and from 416.7 mm to 1102.7 mm, with the means of 514.8 h, 22.3°C and 719.0 mm, respectively. At the scale of growing period of flue-cured tobacco, the annual cumulative S were ranged from 70.0 h to 257.2 h, from 77.3 h to 322.5 h and from 131.2 h to 300.3 h in the periods of rooting, flourishing and maturing with the means of 127.8 h, 190.4 h and 197.2 h, respectively. The mean temperatures were ranged from 14.2°C to 18.9°C, from 22.2°C to 26.1°C, and from 25.8°C to 29.6°C with the means of 16.4°C, 24.1°C and 28.0°C, respectively. The annual cumulative P were ranged from 69.9 mm to 553.9 mm, from 133.1 mm to 485.6 mm and from 46.4 mm to 356.8 mm in the periods of rooting, flourishing and maturing with the means of 262.5 mm, 299.9 mm and 153.9 mm, respectively. At the year scale, cumulative S annually decreased by 7.587 h, mean T annually increased by 0.02°C, while cumulative P changed irregularly with insignificantly increasing about by 0.184 mm annually. At the scale of whole field period of flue-cured tobacco, mean T annually increased by 0.0195°C, while both cumulative S and P changed irregularly with insignificantly decreasing by 1.64 h and 1.62 mm annually, respectively. At the scale of growing period of flue-cured tobacco, all climatic parameters changed irregularly except cumulative S in significant positive compound function in the maturing period. Meanwhile, the slight decreasing tendency of cumulative sunshine hours and the slight increasing tendency of cumulative precipitation are disadvantageous while the slight increasing tendency of cumulative precipitation in the maturing period is positive for the growth and quality of flue-cured tobacco.

不同施氮量与打顶时间对烤烟生长、化学成分和经济效益的影响

不同施氮量与打顶时间对烤烟生长、化学成分和经济效益的影响

不同氢氧化镁施用量对烤烟生长、化学成分和经济性状的影响

不同氢氧化镁施用量对烤烟生长、化学成分和经济性状的影响

不同播种期对烤烟生长、质量和经济效益的影响

不同播种期对烤烟生长、质量和经济效益的影响

Time-course relationship between environmental factors and microbial diversity in tobacco soil

Soil physicochemical properties and microbial diversity both play equally important roles in tobacco cultivation. However, the relationship between these factors remains unclear. In this study, we investigated their correlations through the whole tobacco growth period, including the pretransplanting (YX-p), root extending (R), flourishing (F), and mature (M) stages in the Yuxi region of the Yunnan-Guizhou Plateau by measuring physicochemical properties and conducting 16S/18S rRNA analysis. The analysis demonstrated that the microbial community richness and diversity continuously changed along with the growth course of the tobacco. Multiple environmental factors showed a certain correlation with the diversity of microbial communities. Some bacteria could accumulate nitrogen during the growth stages, and the diversity of the bacterial community also increased when the content of organic matter rose. In addition, the water content and available K also influenced the diversity of the microbial community. The dynamic changes in soil physicochemical properties and enzyme activities gave rise to differences in the microbial community composition and structure, all of which affected the growth of tobacco. This study revealed the time-course relationship between environmental factors and microbial diversity in tobacco soil. An understanding of this relationship provides guidance for research on the interaction system of plants, soil and microbes and on improving plant yield and quality.