Sheath Growth Research Articles

Experiment research of post-arc current and cathode spots distribution in medium-high frequency vacuum arc

In this paper, the breaking process of the vacuum circuit breakers is briefly described, and the main factors that influence the post-arc process are analyzed. Based on the detachable vacuum interrupter, the experimental platform of AC breaking is built, and the experiments of the post-arc current measurement and cathode spot observation are completed. The voltage and current during the period of current zero are measured, and the images of cathode spots during the arcing process are obtained. The experimental results show that at the same frequency, the post-arc current Ipac and the post-arc charge Qpac increase with the increase in didtcz(didt at current zero). Under the same conditions of didtcz, Ipac and Qpac decrease with the increase in frequency, but both are unchanged with frequency when the frequency is high enough. With the increase in current frequency, the distribution diameter of cathode spots decreases under the condition of the same didtcz value. According to the characteristics of cathode spot distribution, the metal vapor density under different conditions is calculated. Comparing the calculation results of metal vapor with the experimental results of post-arc current, the concept of metal vapor ionization is put forward. An ionization process of metal vapor after the current zero crossing occurs. The ionization of metal vapor will cause an increase in the number of electrons after the current zero crossing, which affects Ipac, Qpac, and sheath growth.

Mechanism of GA-mediated leaf sheath growth in rice: a proteomic approach

Gibberellins (GAs) mediated elongation growth and its associated proteomics in rice is relevant to crop productivity in many diversified ecological conditions. There is dearth of precise investigations conducted on proteome dynamics involved in leaf sheath elongation in both wild and mutant rice plants in response to GAs treatments. To explore the molecular mechanism of GA-induced leaf sheath elongation in rice, we conducted a comparative proteomics analysis of wild type rice and a GA biosynthesis deficient mutant osko2. The mutant displayed an obvious dwarfism phenotype, which could be dramatically rescued by application of exogenous GA3. The average levels of GAs in the dwarf mutant plants were lower than the wild type of rice. The proteomic analysis showed a total of 6352 proteins that were quantified, among which 688 differentially expressed proteins were identified as GA-responsive. The biological process classification indicated that metabolic process, cellular process and single-organism process related proteins were the dominant protein groups, and the differentially expressed proteins were largely located in chloroplast, cytoplasm, and nucleus and extracellular. Furthermore, these proteins were mainly involved in cell elongation, signal transduction, photosynthesis, and carbohydrate and protein metabolism. These results suggested that proteins involved in cell elongation, signal transduction and carbohydrate metabolism are regulated by GA in rice leaf-sheath growth.

Oligodendrocyte Neurofascin Independently Regulates Both Myelin Targeting and Sheath Growth in the CNS.

SummarySelection of the correct targets for myelination and regulation of myelin sheath growth are essential for central nervous system (CNS) formation and function. Through a genetic screen in zebrafish and complementary analyses in mice, we find that loss of oligodendrocyte Neurofascin leads to mistargeting of myelin to cell bodies, without affecting targeting to axons. In addition, loss of Neurofascin reduces CNS myelination by impairing myelin sheath growth. Time-lapse imaging reveals that the distinct myelinating processes of individual oligodendrocytes can engage in target selection and sheath growth at the same time and that Neurofascin concomitantly regulates targeting and growth. Disruption to Caspr, the neuronal binding partner of oligodendrocyte Neurofascin, also impairs myelin sheath growth, likely reflecting its association in an adhesion complex at the axon-glial interface with Neurofascin. Caspr does not, however, affect myelin targeting, further indicating that Neurofascin independently regulates distinct aspects of CNS myelination by individual oligodendrocytes in vivo.

Developmental stage-specific role of Frs adapters as mediators of FGF receptor signaling in the oligodendrocyte lineage cells.

FGF signaling is important for numerous cellular processes and produces diverse cellular responses. Our recent studies using mice conditionally lacking FGF-Receptor-1 (Fgfr1) or Fgfr2 during different stages of myelinogenesis revealed that Fgfr signaling is first required embryonically for the specification of oligodendrocyte progenitors (OPCs) and then later postnatally for the growth of the myelin sheath during active myelination but not for OPC proliferation, differentiation, or ensheathment of axons. What intracellular signal transduction pathways are recruited immediately downstream of Fgfrs and mediate these distinct developmentally regulated stage-specific responses remain unclear. The adapter protein Fibroblast-Growth-Factor-Receptor-Substrate-2 (Frs2) is considered a key immediate downstream target of Fgfrs. Therefore, here, we investigated the in vivo role of Frs adapters in the oligodendrocyte lineage cells, using a novel genetic approach where mice were engineered to disrupt binding of Frs2 to Fgfr1 or Fgfr2, thus specifically uncoupling Frs2 and Fgfr signaling. In addition, we used conditional mutants with complete ablation of Frs2 and Frs3. We found that Frs2 is required for specification of OPCs in the embryonic telencephalon downstream of Fgfr1. In contrast, Frs2 is largely dispensable for transducing Fgfr2-mediated signals for the growth of the myelin sheath during postnatal myelination, implying the potential involvement of other adapters downstream of Fgfr2 for this function. Together, our data demonstrate a developmental stage-specific function of Frs2 in the oligodendrocyte lineage cells. This contextual requirement of adapter proteins, downstream of Fgfrs, could partly explain the distinct responses elicited by the activation of Fgfrs during different stages of myelinogenesis.

The RNA binding protein fragile X mental retardation protein promotes myelin sheath growth.

During development, oligodendrocytes in the central nervous system extend a multitude of processes that wrap axons with myelin. The highly polarized oligodendrocytes generate myelin sheaths on many different axons, which are far removed from the cell body. Neurons use RNA binding proteins to transport, stabilize, and locally translate mRNA in distal domains of neurons. Local synthesis of synaptic proteins during neurodevelopment facilitates the rapid structural and functional changes underlying neural plasticity and avoids extensive protein transport. We hypothesize that RNA binding proteins also regulate local mRNA regulation in oligodendrocytes to promote myelin sheath growth. Fragile X mental retardation protein (FMRP), an RNA binding protein that plays essential roles in the growth and maturation of neurons, is also expressed in oligodendrocytes. To determine whether oligodendrocytes require FMRP for myelin sheath development, we examined fmr1-/- mutant zebrafish and drove FMR1 expression specifically in oligodendrocytes. We found oligodendrocytes in fmr1-/- mutants developed myelin sheaths of diminished length, a phenotype that can be autonomously rescued in oligodendrocytes with FMR1 expression. Myelin basic protein (Mbp), an essential myelin protein, was reduced in myelin tracts of fmr1-/- mutants, but loss of FMRP function did not impact the localization of mbpa transcript in myelin. Finally, expression of FMR1-I304N, a missense allele that abrogates FMRP association with ribosomes, failed to rescue fmr1-/- mutant sheath growth and induced short myelin sheaths in oligodendrocytes of wild-type larvae. Taken together, these data suggest that FMRP promotes sheath growth through local regulation of translation.

Oligodendrocytes express synaptic proteins that modulate myelin sheath formation

Vesicular release from neurons promotes myelin sheath growth on axons. Oligodendrocytes express proteins that allow dendrites to respond to vesicular release at synapses, suggesting that axon-myelin contacts use similar communication mechanisms as synapses to form myelin sheaths. To test this, we used fusion proteins to track synaptic vesicle localization and membrane fusion in zebrafish during developmental myelination and investigated expression and localization of PSD95, a dendritic post-synaptic protein, within oligodendrocytes. Synaptic vesicles accumulate and exocytose at ensheathment sites with variable patterning and most sheaths localize PSD95 with patterning similar to exocytosis site location. Disruption of candidate PDZ-binding transsynaptic adhesion proteins in oligodendrocytes cause variable effects on sheath length and number. One candidate, Cadm1b, localizes to myelin sheaths where both PDZ binding and extracellular adhesion to axons mediate sheath growth. Our work raises the possibility that axon-glial communication contributes to myelin plasticity, providing new targets for mechanistic unraveling of developmental myelination.

Radiographic Soft Tissue Thickness Differentiating Pyogenic Flexor Tenosynovitis From Other Finger Infections

Characteristic swelling has been described as a differentiating sign of pyogenic flexor tenosynovitis (PFT) but has not been validated. We conducted a retrospective study of adults with finger infections to compare radiographic parameters of soft tissue dimensions. Our hypothesis was that in patients with digit infections, radiographic soft tissue thickness measurement would differ between PFT and non-PFT infected digits. Patients with a finger infection and radiographic evaluation were identified retrospectively ata large academic medical center and divided into 2 groups: PFT (n= 31) and non-PFT infections (n= 31). We defined PFT as purulence in the tendon sheath or positive culture growth from the sheath at surgery. Non-PFT infections included all other finger infections such as abscesses and cellulitis. Atotal of 15 radiographic measurements were made on all included digits. Ratios and differences were calculated to characterize the pattern of swelling for each infected finger. Bivariate analysis wasperformed to identify potential predictor variables between the PFT and non-PFT groups. Logisticregression was performed to reduce confounding and model potential relationships. Neither presence of diffuse swelling nor the shape of finger swelling distinguished PFT from non-PFT infections. All finger infections resulted in diffuse swelling. Pyogenic flexor tenosynovitis was distinguished by differential volar soft tissue thickness minus dorsal soft tissue thickness on radiographs at the proximal phalanx level (9 ± 1 mm for PFT vs 5 ± 1 mm for non-PFT). This was an independent predictor of PFT. The area under the receiver operating curve was 0.83 (95% confidence interval, 0.73-0.94). A difference between volar and dorsal soft tissue swelling of 7 mm or greater had a positive predictive value of 82% with a sensitivity of 84% and specificity of 74%. A difference of 10 mm predicted PFT infection with 76% probability (95% confidence interval, 73% to 99%). Pyogenic flexor tenosynovitis may result in uniform finger swelling, but this does not appear to distinguish PFT from other finger infections. Acute PFT swelling is distinguished by differential volar versus dorsal radiographic soft tissue thickness at the level of the proximal phalanx. The term "fusiform swelling" is a misnomer for the appearance of acute PFT because the finger is not spindle-shaped. Diagnostic IV.

Independent and cooperative roles of the Mek/ERK1/2-MAPK and PI3K/Akt/mTOR pathways during developmental myelination and in adulthood.

Multiple extracellular and intracellular signals regulate the functions of oligodendrocytes as they progress through the complex process of developmental myelination and then maintain a functionally intact myelin sheath throughout adult life, preserving the integrity of the axons. Recent studies suggest that Mek/ERK1/2-MAPK and PI3K/Akt/mTOR intracellular signaling pathways play important, often overlapping roles in the regulation of myelination. However, it remains poorly understood whether they function independently, sequentially, or converge using a common mechanism to facilitate oligodendrocyte differentiation, myelin growth, and maintenance. To address these questions, we analyzed multiple genetically modified mice and asked whether the deficits due to the conditional loss-of-function of ERK1/2 or mTOR could be abrogated by simultaneous constitutive activation of PI3K/Akt or Mek, respectively. From these studies, we concluded that while PI3K/Akt, not Mek/ERK1/2, plays a key role in promoting oligodendrocyte differentiation and timely initiation of myelination through mTORC1 signaling, Mek/ERK1/2-MAPK functions largely independently of mTORC1 to preserve the integrity of the myelinated axons during adulthood. However, to promote the efficient growth of the myelin sheath, these two pathways cooperate with each other converging at the level of mTORC1, both in the context of normal developmental myelination or following forced reactivation of the myelination program during adulthood. Thus, Mek/ERK1/2-MAPK and the PI3K/Akt/mTOR signaling pathways work both independently and cooperatively to maintain a finely tuned, temporally regulated balance as oligodendrocytes progress through different phases of developmental myelination into adulthood. Therapeutic strategies aimed at targeting remyelination in demyelinating diseases are expected to benefit from these findings.

Mechanism of Dynamic Voltage Distribution in Series-Connected Vacuum Interrupters

This paper proposes the equivalent circuit of postarc dynamic voltage distribution (PDVD) in multibreak vacuum circuit breakers (VCBs). The equivalent circuit is composed of controlled postarc sources, postarc resistors, and equivalent capacitors. The sheath growth and the postarc process of double-break VCBs are analyzed. The PDVD is divided into four processes: the initial transient recovery voltage (TRV) process, the oscillating TRV process, the stable TRV process, and the power-frequency voltage process, which can be analyzed by the equivalent circuit. The novel equivalent circuit of the PDVD in multibreak VCBs is gained by introducing the controlled postarc current source. The test platform is set up in the synthetic circuit. The postarc current, grading capacitor current, and the TRV are measured to analyze the PDVD in the above four processes. The test results verify the theoretical analysis. Therefore, the mechanism of the PDVD in multibreak VCBs is obtained and described by the equivalent circuit, which is useful to gain even voltage distribution and improve the breaking capacity of multibreak VCBs.

Prospects of endophyllic Pseudomonas aeruginosa as a biocide against sheath blight and growth promotion of rice

Ecofriendly ecto-/endospheric plant growth promoting microbes (PGPM) and biocides would sustain rice growth and development, save from diseases and pests to keep up rice production for food security and protect the ecosphere. As the pseudomonads are most versatile microbes, the PGP and biocidal potential of a multifaceted endophyllic pseudomonad (JE1) of rice were evaluated. Phenotyping, FAME and 16S rDNA homology confirmed identity of the organism as Pseudomonas aeruginosa. The organism possessed the plant growth promotion (PGP) functions like P solubilization (536.96 μg/ml), ACC, siderophore (7.77 mg/g dr. wt.), antimicrobial metabolite viz. HCN (0.035 A625/ml) etc. and antimicrobial enzyme viz. pectinase, tributyrin hydrolase, gelatinase and cellulase. Besides, the organism inhibited growth of rice sheath blight (ShB) pathogen Rhizotocnia solani by 35.89%. The PGP and antimicrobial functionalities proved versatility of the P. aeruginosa (JE1) and potential for overall improvement/sustenance of rice health and production.

Characteristics and Influencing Factors of the Postarc Current in Vacuum DC Interruption

The postarc current (PAC) is an important parameter, which can indicate the interrupting capacity of breakers. According to the amplitude of the PAC, the appearance of the arc before current zero and the quantity of residual metal vapor particles in the electrode distance after current zero can be studied qualitatively. In order to investigate the characteristics of PAC in direct current (dc) interruption, a high-speed hybrid actuator was designed, and the forced current zero method was used to carry out a series of dc interrupting experiments in the synthetic test circuit. Also, the equipment for measuring the PAC was established to investigate the factors influencing on the PAC, including the amplitude of rated breaking current, electrode distance, and commutation frequency. The experimental results indicated that PAC presented to be instable in a certain range, due to the different contact structures, arcing positions, and so on. To some extent, the instability of PAC reflects the sheath growth process after the current zero. In addition, the amplitude of PAC changes with the variation of electrode distances. For the same distances, PAC keeps stable in a certain range. When the rated breaking current is less than 800 A, PAC increases with a high growth rate. However, the one increases with a low growth rate when the rated breaking current varies within 800–2000A. For different commutation frequencies, PAC has a significant growth with the increase of the commutation frequency. It is for the reason that more residual metal vapor particles exist in the electrode distance after current zero when the commutation frequency is higher, which goes against to dc interruption.

Calcium control of myelin sheath growth.

New studies provide compelling evidence that the number and length of myelin sheaths generated by oligodendrocytes in the CNS are controlled by local calcium levels, linking axonal activity to individual myelin sheath formation.

Ca 2+ activity signatures of myelin sheath formation and growth in vivo.

During myelination, individual oligodendrocytes initially over-produce short myelin sheaths that are either retracted or stabilised. By live imaging oligodendrocyte Ca2+ activity in vivo, we find that high-amplitude long-duration Ca2+ transients in sheaths prefigure retractions, mediated by calpain. Following stabilisation, myelin sheaths grow along axons, and we find that higher frequency Ca2+ transient activity in sheaths precedes faster elongation. Our data implicate local Ca2+ signalling in regulating distinct stages of myelination.

Helminthosporic acid functions as an agonist for gibberellin receptor.

Helminthosporol was isolated from a fungus, Helminthosporium sativum, as a natural plant growth regulator in 1963. It showed gibberellin-like bioactivity that stimulated the growth of the second leaf sheath of rice. After studying the structure-activity relationship between the compound and some synthesized analogs, it was found that helminthosporic acid (H-acid) has higher gibberellin-like activity and chemical stability than helminthosporol. In this study, we showed that (1) H-acid displays gibberellin-like activities not only in rice but also in Arabidopsis, (2) it regulates the expression of gibberellin-related genes, (3) it induces DELLA degradation through binding with a gibberellin receptor (GID1), and (4) it forms the GID1-(H-acid)-DELLA complex to transduce the gibberellin signal in the same manner as gibberellin. This work shows that the H-acid mode of action acts as an agonist for gibberellin receptor.

Development of biomimetic micro-patterned device incorporated with neurotrophic gradient and supportive Schwann cells for the applications in neural tissue engineering

In these years, the artificial nerve guidance conduit (NGC) has been developed as an alternative way to repair peripheral nerve injury. Unlike autologous nerve graft, the artificial NGC without proper stimulating factors and guidance cues still cannot obtain satisfactory prognosis for clinical patients. In this study, a biodegradable polymer-based implantable device has been developed and characterized. By incorporating three stimulating factors: (1) micro-patterned surface that can directionally guide the axon as physical cue; (2) neurotrophic gradient membrane that can continually attract axon outgrowth from the proximal to distal stump as chemical cue; (3) Schwann cells (SCs) that can support the growth of neurite and form myelin sheath around axon as biological cue, we expect that this construct can be used as a promising NGC for peripheral nerve regeneration. The results showed that the micro-patterned surface with specific dimension of channels and chambers can be precisely fabricated by laser ablation. Attachment and directional extension of differentiated neural stem cells (NSCs) were observed in micro-channels. The gradient distribution of nerve growth factor 7S on gelatin membrane was successfully achieved. Significant improvement in neurite length and increase in neuronal gene expressions were also noticed in higher concentration region. When co-culturing with SCs, NSCs can differentiate toward neuronal cells with strong expression of mature neuronal markers: βIII tubulin and microtubule-associated protein-2 (Map 2). Meanwhile, myelin basic protein was also observed, suggesting that SCs can provide biological support to neuronal cells in vitro. In the future, this advanced artificial NGC may be used as implantable prosthesis for the treatment of peripheral nerve injury with better functional recovery.

Signaling by FGF Receptor 2, Not FGF Receptor 1, Regulates Myelin Thickness through Activation of ERK1/2-MAPK, Which Promotes mTORC1 Activity in an Akt-Independent Manner.

FGF signaling has emerged as a significant "late-stage" regulator of myelin thickness in the CNS, independent of oligodendrocyte differentiation. Therefore, it is critically important to identify the specific FGF receptor type and its downstream signaling molecules in oligodendrocytes to obtain better insights into the regulatory mechanisms of myelin growth. Here, we show that FGF receptor type 2 (FGFR2) is highly enriched at the paranodal loops of myelin. Conditional ablation of this receptor-type, but not FGF receptor type 1 (FGFR1), resulted in attenuation of myelin growth, expression of major myelin genes, key transcription factor Myrf and extracellular signal-regulated protein kinase 1 and 2 (ERK1/2) activity. This was rescued by upregulating ERK1/2 activity in these mice, strongly suggesting that ERK1/2 are key transducers of FGFR2 signals for myelin growth. However, given that the PI3K/Akt/mechanistic target of rapamycin (mTOR) pathway is also known to regulate myelin thickness, we examined FGFR2-deficient mice for the expression of key signaling molecules in this pathway. A significant downregulation of p-mTOR, p-Raptor, and p-S6RP was observed, which was restored to normal by elevating ERK1/2 activity in these mice. Similar downregulation of these molecules was observed in ERK1/2 knock-out mice. Interestingly, since p-Akt levels remained largely unchanged in these mice, it suggests a mechanism of mTORC1 activation by ERK1/2 in an Akt-independent manner in oligodendrocytes. Taken together, these data support a model in which FGFs, possibly from axons, activate FGFR2 in the oligodendrocyte/myelin compartment to increase ERK1/2 activation, which ultimately targets Myrf, as well as converges with the PI3K/Akt/mTOR pathway at the level of mTORC1, working together to drive the growth of the myelin sheath, thus increasing myelin thickness.SIGNIFICANCE STATEMENT It is well accepted that myelin is a biologically active membrane in active communication with the axons. However, the axonal signals, the receptors on myelin, and the integration of intracellular signaling pathways emanating downstream from these receptors that drive the growth of the myelin sheath remain poorly understood in the CNS. This study brings up the intriguing possibility that FGF receptor 2, in the oligodendrocyte/myelin compartment, may be one such signal. Importantly, it provides compelling evidence linking FGFR2 with the ERK1/2-MAPK pathway, which converges with the PI3K/Akt/mTOR (mechanistic target of rapamycin) pathway at the level of mTORC1 and also regulates the transcription factor Myrf, together providing a mechanistic framework for regulating both the transcriptional and translational machinery required for the proper growth of the myelin sheath.

Epidermal Growth Factor Promotes Proliferation and Migration of Follicular Outer Root Sheath Cells via Wnt/β-Catenin Signaling

Background/Aims: To investigate the effect and molecular mechanism of EGF on the growth and migration of hair follicle outer root sheath (ORS) cells. Methods: Intact anagen hair follicles were isolated from mink skin and cultured with EGF in vitro to measure ORS daily growth. Meanwhile, purified primary ORS cells were treated or transfected with EGF, and their proliferation and migration were assessed by MTT assay and transwell assay, respectively. The signaling pathway downstream of EGF was characterized by using the Wnt/β-catenin signaling inhibitor, XAV-939. Results: EGF of 2-20 ng/ml, not higher or lower, promoted the growth of follicular ORS in vitro. EGF treatment or overexpression promoted the proliferation and migration of ORS cells. Moreover, EGF stimulation induced nuclear translocation of β-catenin, and upregulated the expression of Wnt10b, β-catenin, EGF receptor and SOX9. Inhibition of Wnt/β-catenin signaling by XAV-939 significantly reduced the basal and EGF-enhanced proliferation and migration of ORS cells. In addition, a number of follicle-regulatory genes, such as Survivin, Msx2 and SGK3, were upregulated by EGF in the ORS cells, which was also inhibited by XAV-939. Conclusion: EGF promotes the proliferation and migration of ORS cells and modulates the expression of several follicle-regulatory genes via Wnt/β-catenin signaling.

Neuronal activity biases axon selection for myelination in vivo.

An essential feature of vertebrate neural development is ensheathment of axons with myelin, an insulating membrane formed by oligodendrocytes. Not all axons are myelinated, but mechanisms directing myelination of specific axons are unknown. Using zebrafish we show that activity-dependent secretion stabilizes myelin sheath formation on select axons. When VAMP2-dependent exocytosis is silenced in single axons, oligodendrocytes preferentially ensheath neighboring axons. Nascent sheaths formed on silenced axons are shorter in length, but when activity of neighboring axons is also suppressed, inhibition of sheath growth is relieved. Using in vivo time-lapse microscopy, we show that only 25% of oligodendrocyte processes that initiate axon wrapping are stabilized during normal development, and that initiation does not require activity. Instead, oligodendrocyte processes wrapping silenced axons are retracted more frequently. We propose that axon selection for myelination results from excessive and indiscriminate initiation of wrapping followed by refinement that is biased by activity-dependent secretion from axons.

Erythropoietin promotes peripheral nerve regeneration in rats by upregulating expression of insulin-like growth factor-1.

IntroductionErythropoietin (EPO) has been shown to have beneficial effects on peripheral nerve damage, but its mechanism of action remains incompletely understood. In this study we hypothesized that EPO promotes peripheral nerve repair via neurotrophic factor upregulation.Material and methodsThirty adult male Wistar rats were employed to establish a sciatic nerve injury model. They were then randomly divided into two groups to be subjected to different treatment: 0.9% saline (group A) and 5000 U/kg EPO (group B). The walking behavior of rats was evaluated by footprint analysis, and the nerve regeneration was assessed by electron microscopy. The expression of insulin-like growth factor-1 (IGF-1) in the injured sciatic nerves was detected by immunohistochemical analysis.ResultsCompared to saline treatment, EPO treatment led to the growth of myelin sheath, the recovery of normal morphology of axons and Schwann cells, and higher density of myelinated nerve fibers. Erythropoietin treatment promoted the recovery of SFI in the injured sciatic nerves. In addition, EPO treatment led to increased IGF-1 expression in the injured sciatic nerves.ConclusionsErythropoietin may promote peripheral nerve repair in a rat model of sciatic nerve injury through the upregulation of IGF-1 expression. These findings reveal a novel mechanism underlying the neurotrophic effects of EPO.

Ultra-thin flexible polyimide neural probe embedded in a dissolvable maltose-coated microneedle

The ultra-thin flexible polyimide neural probe can reduce the glial sheath growth on the probe body while its flexibility can minimize the micromotion between the probe and brain tissue. To provide sufficient stiffness for penetration purposes, we developed a drawing lithography technology for uniform maltose coating to make the maltose-coated polyimide neural probe become a stiff microneedle. The coating thicknesses under different temperature and the corresponding stiffness are studied. It has been proven that the coated maltose is dissolved by body fluids after implantation for a few seconds. Moreover, carbon nanotubes are coated on the neural probe recording electrodes to improve the charge delivery ability and reduce the impedance. Last but not least, the feasibility and recording characteristic of this ultra-thin polyimide neural probe embedded in a maltose-coated microneedle are further demonstrated by in vivo tests.