A field experiment was carried out in the season 2021–2022 in the Dora/Baghdad area on lettuce plants and included the treatment of soil with two levels of poultry waste (0,12) tons ha−1, phosphorus added at three levels (0, 100, 120) kg P ha−1 and nitrogen at three levels (0, 120, 180) kg N ha−1. The results showed a significant increase in leaf area and chlorophyll when adding fermented poultry waste, and the treatment of fertilization with poultry waste was superior to 670 Dcm2 and 40.60 for both leaf area and chlorophyll, respectively, and a significant increase was found when fertilizing with poultry waste. In urea phosphate levels, significant differences were recorded, while no significant differences were recorded in chlorophyll, and the fertilization with ammonium sulfate levels recorded significant differences for the two indicators, and the interaction treatment p1A3U3 recorded the highest values, which were 793 Dcm2 and 45.16 for each leaf area and chlorophyll, respectively, and there was a significant increase in the biological yield and economic yield of lettuce. When adding fermented poultry waste, the treatment of fertilization with poultry waste was recorded as 3237 gm plant−1 and 1678 gm plant−1 for both biological yield and economic yield, respectively. significant increase when fertilizing with urea phosphate levels and with significant differences, and the addition of ammonium sulfate showed significant increases for the two indicators, and the interaction treatment p1A3U3 recorded the highest values, and it was 4250 gm plant−1 and 2200 gm plant−1 for both biological yield and economic yield, respectively, and there was a significant increase in the proportion of protein and beta-carotene When adding fermented poultry waste, the treatment of fertilizing with poultry waste was superior to 1.35 gm of 100 g−1 and 1.330gm 100ml−1 juice for both protein and beta-carotene ratios, respectively. A significant increase was also found when fertilizing with urea phosphate levels with significant differences, and the addition of ammonium sulfate, which resulted in significant increases for the two indicators. And the interaction p1A3U3 treatment recorded the highest values, which were 1.50 g for 100 gm−1 and 1.853 gm for 100 ml−1 juice for each of the protein and beta-carotene ratios, respectively.