Length Growth Research Articles

Sanxiapeptin is an ideal preservative with a dual effect of controlling green mold and inducing systemic defense in postharvest citrus

Green mold is a common postharvest disease infected by Penicillium digitatum that causes citrus fruit decay, and severely affects fruit storage quality. This work aimed to investigate the antifungal activity of Sanxiapeptin against P. digitatum, and elucidate the possible mechanisms involved. Sanxiapeptin was capable of inhibiting spore germination, germ tube length and mycelial growth. The SYTOX green staining assay revealed that Sanxiapeptin targeted the fungal membrane, and changed the membrane permeability, leading to the leakage of cell constituents. Meanwhile, Sanxiapeptin could influence the cell wall permeability and integrity by increasing the activities of chitinase and glucanase, resulting in abnormal chitin consumption and the decrease of glucan. Intriguingly, Sanxiapeptin could effectively control postharvest decay in citrus fruits, and activate the host resistance responses by regulating the phenylpropanoid pathway. In conclusion, Sanxiapeptin exhibits multiphasic antifungal mechanisms of action to control green mold in citrus fruits, shows great potential as novel food preservatives.

Influence of morbidity, early nutritional intake, and total energy: protein ratio on longitudinal extrauterine growth restriction of very preterm newborns at term-equivalent age: an observational study.

To evaluate the influence of early nutritional intake on the growth pattern of very preterm infants. This was an observational study including 109 newborns (< 32weeks gestational age). Perinatal morbidities, nutritional therapy (first four weeks of life), and weight, length, and head circumference (HC) growth at term-equivalent age were evaluated. Growth restriction was defined as a difference > 1.2 SD between the birth and term age measurements. Growth restriction at term-equivalent age: 52.3% (weight), 42.9% (length), and 22% (HC). Morbidities were positively correlated with nutrition therapy and negatively correlated with the total energy provision: protein ratio. The duration of parenteral nutrition, the time to reach full enteral feedings, and the total energy provision: protein ratio were significantly correlated. Nutrient intake influenced weight, length, and HC growth, and cumulative energy deficit was significantly associated with HC growth restriction. Conclusion: Perinatal morbidities interfere with nutritional therapy and early nutrient intake, leading to insufficient energy and energy provision: protein ratio for growth. What is Known: • The intake of macronutrients early in life, mainly protein, is important for the optimal growth of pretem infants. • The severity of morbidities and low gestational ages impact the nutritional management of preterm infants. What is New: • The number of morbidities, reflecting the severity of the neonatal clinical course, had a detrimental effect on the nutritional therapy and nutrients intake. • The inadequate energy provision per gram of protein ratio was significantly associated with growth restriction in all growth measures at the second week of life, persisting for head circumference up to the fourth week, highlighting the importance of its measurement, as it could be a precocious sign of development risk.

Novel semi-analytical model for solving the C*-integral of specimens with mode-I crack under various constraints

The energy rate line integral C* is an essential fracture parameter that characterizes the stress and strain rate field intensity at a crack tip for materials undergoing creep deformation, and is also a crucial parameter describing the creep crack growth rate. This study proposes a method for determining the characteristic parameters of the creep displacement rate model for specimens with mode-I crack under various constraints. Based on the displacement rate model, a semi-analytical model of the C*-integral related to the displacement rate, load, specimen sizes, and Norton's law parameters of materials is proposed. Furthermore, finite element analysis (FEA) models were established for compact tension (CT), single-edged notched bending (SEB), C-shaped tension (CST), and single-edge notched tension (SET) specimens, and the proposed models were verified using FEA results and those predicted by the ASTM method. The explicit correlation between the creep displacement rate and crack length a in the model can contribute to developing novel testing methods for real-time crack length and creep crack growth rate.

Genetic Basis for Morphological Variation in the Zebrafish Danio rerio: Insights from a Low-Heterozygosity Line

Data variability complicates reproducibility and the interpretation of experimental results. Different animal models have been employed to decrease variability to enhance experimental power. However, variation frequently persists among and within strains/lines. In zebrafish (Danio rerio), inbred lines (e.g., NHGRI-1) derived from wild-type lines have been produced to greatly decrease genetic variation, with the goal of providing better understanding of genetic backgrounds that may influence the experimental outcome of studies employing such lines. We hypothesized that variations in morphological phenotypes shaped by environmental stressors early in development are correlated with the intrinsic degree of genetic variability of zebrafish lines. We compared morphological variability (yolk–chorion ratio, body mass, embryo mass, total length, condition factor, and specific growth rate) in wild-type AB and NHGRI-1 zebrafish lines as a function of their responses to altered temperature and oxygen availability during the first 7 days post-fertilization. Overall, both lines showed similar developmental trajectories for yolk–chorion ratio, embryo mass, and total length. Additionally, condition factor and specific growth rate showed similar responses within each line, regardless of temperature and hypoxia. Importantly, the coefficient of variation for each variable was significantly lower in NHGRI-1 than AB larvae for 151 of 187 assessed morphological endpoints. Thus, the low-heterozygosity NHGRI-1 zebrafish line can be useful for decreasing inter-individual variation in morphological responses to environmental stressors, thereby aiding in the interpretation of results and enhancing experimental reproducibility.

Formulasi Sediaan Hair Tonic Ekstrak Rumput Laut (Hormophysa Triquetra) Dengan Kombinasi Ekstrak Kemiri (Aleurites Moluccana) Sebagai Penumbuh Rambut

Hormophysa triquetra seaweed is one of the marine natural resources that has not been utilized optimally by society. H. triquetra seaweed has a dominant pigment, namely fucoxanthin, which has anti-cancer and anti-obesity properties. Seaweed is widely used as a cosmetic product by utilizing its ingredients. H. triquetra seaweed contains flavonoids, phenols as antioxidants which are able to repair damaged hair cells and produce skin tissue which is conducive to hair growth, besides the vitamin C content which can increase blood circulation and provide nutrition for hair follicles. Aleurites moluccana candlenut oil contains high levels of oleic acid and has activity as a hair growth stimulant which has been widely used for generations as a hair tonic. In addition, the antioxidant properties of candlenut oil's oleic acid are believed to slow hair loss and accelerate hair growth. The aim of this research is to determine the formulation and characteristics of the best hair tonic made from H. triquetra seaweed and candlenut extract as a hair growth agent. A completely randomized design with 4 treatments and 3 replications was used in this experiment. The best results were in the F0 treatment because it showed good physical stability in the test animals and fast hair length growth among other treatments because the samples used contained the highest seaweed extract and contained antioxidants which could protect hair and maintain good hair pigmentation.

Bruno 1/CELF regulates splicing and cytoskeleton dynamics to ensure correct sarcomere assembly in Drosophila flight muscles.

Muscles undergo developmental transitions in gene expression and alternative splicing that are necessary to refine sarcomere structure and contractility. CUG-BP and ETR-3-like (CELF) family RNA-binding proteins are important regulators of RNA processing during myogenesis that are misregulated in diseases such as Myotonic Dystrophy Type I (DM1). Here, we report a conserved function for Bruno 1 (Bru1, Arrest), a CELF1/2 family homolog in Drosophila, during early muscle myogenesis. Loss of Bru1 in flight muscles results in disorganization of the actin cytoskeleton leading to aberrant myofiber compaction and defects in pre-myofibril formation. Temporally restricted rescue and RNAi knockdown demonstrate that early cytoskeletal defects interfere with subsequent steps in sarcomere growth and maturation. Early defects are distinct from a later requirement for bru1 to regulate sarcomere assembly dynamics during myofiber maturation. We identify an imbalance in growth in sarcomere length and width during later stages of development as the mechanism driving abnormal radial growth, myofibril fusion, and the formation of hollow myofibrils in bru1 mutant muscle. Molecularly, we characterize a genome-wide transition from immature to mature sarcomere gene isoform expression in flight muscle development that is blocked in bru1 mutants. We further demonstrate that temporally restricted Bru1 rescue can partially alleviate hypercontraction in late pupal and adult stages, but it cannot restore myofiber function or correct structural deficits. Our results reveal the conserved nature of CELF function in regulating cytoskeletal dynamics in muscle development and demonstrate that defective RNA processing due to misexpression of CELF proteins causes wide-reaching structural defects and progressive malfunction of affected muscles that cannot be rescued by late-stage gene replacement.

Pengaruh Penambahan Ampas Tahu Dan Keong Mas (&lt;i&gt;Pomacea canaliculata&lt;/i&gt;) Pada Ratro Pellet Komersil Terhadap Pertumbuhan Ikan Lele (C&lt;i&gt;larias gariepinus&lt;/i&gt;)

The need for fish food is one of the essentials that can determine the level of success of the fish growth and development process. This research aims to analyze the effect of a combination of pelleted feed and animal feed on the growth and survival of catfish (Clarias gariepinus). The research was carried out for 60 days, at the Mataram University fish production and reproduction laboratory using an experimental method with a completely randomized design (CRD) consisting of 5 treatments and 3 replications, namely: A: 100% commercial feed (control), B: Fish Pellets 50% + Golden Snail 25% + Tofu Dregs 25%, C: Fish Pellets 25% + Golden Snails 50% + Tofu Dregs 25, D: Fish Pellets 25% + Golden Snails 25% + Tofu Dregs 50% and E: Fish Pellets 34% + Keong Mas 33% + Tofu dregs 33%. Test parameters include absolute weight, absolute length, specific weight growth rate, specific length growth rate, feed conversion ratio, feed utilization efficiency and survival and water quality. Data were analyzed using Analysis of Variance (ANOVA) and continued with the Duncan test. The results of this study showed that the best growth and survival of catfish was found in the treatment with 34% commercial feed, 33% golden snails and 33% tofu dregs (P4) with an absolute weight of 15.09 g, an absolute length of 7.05 cm , the specific weight growth rate was 3.86%/day, the length growth rate was 1.3%/day, the feed conversion ratio was 1.18, the feed efficiency rate was 85.91% and the survival rate was 73.33%. The proximate test results showed that the highest content was found in the treatment given 25% commercial feed, 50% golden snails and 25% tofu dregs (P2) with a dry content of 36.39%, crude fiber 10.86% and crude protein 31.48%.

Myopie-Management vom Kind zum Erwachsenen anhand eines Fallberichts

Purpose: The case report describes the successes and lessons learned from optical therapeutic management of progressive myopia, based on long-term treatment plan and the resulting development of the correction and axial lengths of a female patient from age 8 to 22 years. Material and Methods: Using a risk analysis and adaptive care plan, with age-appropriate contact optic care, an attempt was made to positively inhibit, the progression of myopia in a young female patient from 2009 to 2023. The scientific facts and contact lens techniques available at the time of treatment, were applied such as analysis of environmental factors, use of bifocal silicone hydrogel contact lenses and orthokeratology. Results: By using orthokeratology contact lenses, the increase in myopia and axial length growth could be clinically inhibited over several years. Early setbacks during the treatment period due to ineffective measures or early treatment cessation (partial rebound effect) could be stopped or stabilized due to the increasing age of the patient. Conclusion: By means of targeted and controlled contact lens treatment, it is possible to successfully inhibit the progression and thus mitigate the subsequent, secondary health risk.

The impact of exclusive human milk diet on short-term growth of very preterm infants.

The impact of exclusive human milk diet (EHMD) on postnatal growth remains controversial. This study aims to investigate the association between EHMD and short-term growth. This multicenter retrospective study aims to compare growth between the EHMD and non-EHMD groups among infants <32 weeks of gestation. Primary outcomes include weight, length, and head circumference growth trajectories between birth and 34 weeks postmenstrual age. Sensitivity and subgroup analyses were performed. An EHMD was independently associated with poorer length growth, especially in infants born at ≥28 weeks' gestation or those exposed to hypertensive disorders of pregnancy. While initiating fortification at <26 kcal/oz on an EHMD showed inferior growth, initiating fortification at ≥26 kcal/oz was associated with improved weight growth, and similar length and head circumference growth when compared to the non-EHMD group. An EHMD with initial fortification at ≥26 kcal/oz may be implemented to avoid bovine milk exposure while sustaining comparable growth.

The effect of different lighting times on survival and growth rate of gurami seeds (Oshpronemus gouramy)

This study aims to determine the effectiveness of different photoperiods on maturity and the effect of different light durations on survival and growth of gourami (Ospronemus gouramy) fry. The study was conducted on August 22th - September 22th in the Green House Samudra University.Test fish used is seed of gouramy measuring 5-6 cm. The methods used in this study was Completely Randomized Design (CRD) with 4 treatments and 3 replications. The treatments in this study were P1 (4 hours light, 20 hours dark), P2 (8 hours light, 16 hours dark), P3 (12 jam hours light, 12 hours dark), and P4 (16 hours light, 18 hours dark). Seeds of fish was put in a containing 20 liters of water volume with a stocking density of 10 tails each container. The frequency of feeding is done 4 times a day at 06.00 am, 12.00 am, 18 pm and 24.00 pm. The results showed that different natural feeding teratments had a significant effect on the absolute weight, absolute length growth, daily growth rate, survival, FCR, and water quality. The best treatment in this study was P1 treatment (4 hours light, 20 hours dark) because it had a high value compared to other treatments.Keywords: Gouramy; Growth; Photoperiod; Survival; Weight

Exogenous growth hormone (GH) increases dimension and reduces strength of vertebrae in Atlantic salmon (Salmo salar)

Growth hormone (GH) administration increases bone strength in rats, mainly through alterations of the dimensional proportions of vertebrae. However, similar knowledge is lacking in other clades. In order to pursue this, Atlantic salmon were fed diets with high or low vitamin C and E and administered a slow-release implant of bovine GH.While neither the diets or the GH treatment affected growth, GH reduced condition factor and carcass-somatic index, and increased the hepatosomatic and visceral-somatic indexes. GH had an impact on the dimensional proportions of the vertebrae and increased the relative dorso-ventral and lateral vertebra diameters. Moreover, GH decreased the mechanical strength of the vertebra, both with (modulus of elasticity, failure point) and without (stiffness) normalizing the mechanical data for the dimensions of the vertebrae. Diet did not impact on the vertebra morphometry or mechanical strength. RNA-seq of vertebra centra show that several genes associated with bone growth and remodelling were affected by GH, while there was an interactive effect between diet vitamin level and GH on several genes associated with lipid metabolism.The data may suggest that exogenous GH affects the bone cellular mechanisms of Atlantic salmon postsmolts in such a way that their vertebrae increase in diameter but not length, not disturbing growth in body length. The GH and diet effects on lipid metabolism associated genes indicate a complex regulation of the lipids stored inside the vertebral column by endocrine and nutritional factors.

The Effect of Banana Stems (Musa Paradisiaca L.) Toward The Growth of Rice Field Eels (Monopterus Albus) in Cultivation Business

Banana plants have not been utilized optimally. Banana stems can be used as a growth medium for eels. Eels are a type of freshwater animal that has quite high economic value. This research aims to determine the effect of banana stems (Musa paradisiaca L.) on the growth of rice field eels (Monopterus albus). This type of research is quantitative research with experimental methods. The research was conducted on Jl. Biduri Senteluk, Senteluk Village, Batulayar District, West Lombok Regency, NTB in the yard of the house for 3 months. The research design used was a Completely Randomized Design (CRD) with 7 treatments and 3 repetitions. Treatment P0 (100% mud) as control, P1 (90% rice field mud + 10% banana stems), P2 (80% rice field mud + 20% banana stems), P3 (70% rice field mud + 30% banana stems), P4 (60% rice field mud + 40% banana stems), P5 (50% rice field mud + 50% banana stems), and P6 (40% rice field mud + 60% banana stems). The parameters observed were absolute weight, absolute length, and survival rate of rice field eels. The research data were analyzed using the ANOVA test and the BNT test (smallest significant difference). The results of the analysis showed that the addition of banana stems had a significant effect on the growth of rice field eels (P&gt;0.05). The best addition of banana stems was in the P3 treatment, which can be seen from the average value of the weight growth of paddy eels which increased by 6,100 grams and the increase in length growth of paddy eels by 7,200 cm, and the survival rate of paddy eels was 100%.

Effects of responsive breastfeeding intervention on breastfeeding and infant growth in China: A randomised controlled trial.

Responsive feeding serves as an important protective factor for infant growth and overall health development. This study based on self-determination theory (SDT) aimed to assess the effects of a responsive breastfeeding (RBF) intervention programme on maternal breastfeeding and infant growth and development. A total of 110 mother-infant pairs were recruited and randomly divided into an intervention group (n = 55) and a control group (n = 55). The primary outcomes were breastfeeding motivation score, breastfeeding self-efficacy (BSE) and exclusive breastfeeding rate; the secondary outcomes were infant physical development at 6 weeks and 3 months. A repeated measures ANOVA indicated that the intervention group had significantly higher Enjoyment scores compared to the control group at three time points: at discharge (MD: 5.28; 95% CI: 3.68 to 6.89; p < 0.001), 6 weeks post-partum (MD: 5.06; 95% CI: 3.80 to 6.31; p < 0.001) and 3 months post-partum (MD: 5.24; 95% CI: 4.12 to 6.35; p < 0.001). Similarly, the intervention group reported significantly higher connection and mother's self-perception scores at discharge (MD: 4.31; 95% CI: 3.07 to 5.56; p < 0.001), 6 weeks post-partum (MD: 4.69; 95% CI: 3.71 to 5.68; p < 0.001) and 3 months post-partum (MD: 4.93; 95% CI: 4.14 to 5.72; p < 0.001), compared to the control group. In contrast, the pressure from significant others scores were higher in the control group relative to the intervention group at discharge (MD: -2.09; 95% CI: -2.88 to -1.31; p < 0.001), 6 weeks post-partum (MD: -4.35; 95% CI: -5.20 to -3.49; p < 0.001) and 3 months (MD: -4.89; 95% CI: -5.70 to -4.08; p < 0.001). Finally, the intervention group also reported higher Instrumental Needs scores at all three time points: at discharge (MD: 1.96; 95% CI: 1.35 to 2.58; p < 0.001), 6 weeks post-partum (MD: 3.58; 95% CI: 3.05 to 4.11; p < 0.001) and 3 months post-partum (MD: 1.18; 95% CI: 0.68 to 1.69; p < 0.001). BSE scores were significantly higher in the intervention group compared to the control group at discharge (MD: 14.29; 95% CI: 10.38 to 18.21; p < 0.001), 6 weeks post-partum (MD: 14.04; 95% CI: 11.05 to 17.02; p < 0.001) and 3 months post-partum (MD: 6.80; 95% CI: 4.66 to 8.94; p < 0.001). The rates of exclusive breastfeeding were higher in the intervention group than in the control group at each stage of the intervention (p < 0.01). At 6 weeks post-partum, the intervention group's infants showed slower weight (t = -0.90, p = 0.371) and length (t = -0.69, p = 0.495) growth compared to the control group, though not significantly. By 3 months post-partum, there was a significant difference in both weight (t = -3.46, p = 0.001) and length (t = -2.95, p = 0.004) between the groups. The findings in this study suggest that the RBF intervention programme based on SDT may be effective in improving mothers' motivation to breastfeed, building breastfeeding self-confidence and increasing the rate of exclusive breastfeeding. The effects of the intervention on infant physical development will need to be verified with longer follow-up in future research.

Effectiveness of repeated low-level red light in myopia prevention and myopia control

Background/aimsTo compare the effects of repeated low-level red light (RLRL) treatment on axial length growth and refractive error changes in myopic and premyopic children.MethodsSubjects were assigned randomly to four subgroups:...

Fracture ellipticity as a measure of chemical reaction-controlled fracture growth

Fracture ellipticity as a measure of chemical reaction-controlled fracture growth

The Effect of Short-Term Artificial Feed Domestication on the Expression of Oxidative-Stress-Related Genes and Antioxidant Capacity in the Liver and Gill Tissues of Mandarin Fish (Siniperca chuatsi).

To investigate whether Mandarin fish developed oxidative stress after being domesticated with artificial feed, we conducted a series of experiments. Oxidative stress is an important factor leading to diseases and aging in the body. The liver integrates functions such as digestion, metabolism, detoxification, coagulation, and immune regulation, while the gills are important respiratory organs that are sensitive to changes in the water environment. Therefore, we used the liver and gills of Mandarin fish as research materials. The aim of this study was to investigate the effects of short-term artificial feed domestication on the expression of oxidative stress genes and the changes in oxidative-stress-related enzyme activity in the liver and gills of Mandarin fish. We divided the Mandarin fish into two groups for treatment. The control group was fed with live bait continuously for 14 days, while the experimental group was fed with half artificial feed and half live bait from 0 to 7 days (T-7 d), followed by solely artificial feed from 7 to 14 days (T-14 d). The experimental results showed that there was no difference in the body weight, length, and standard growth rate of the Mandarin fish between the two groups of treatments; after two treatments, there were differences in the expression of genes related to oxidative stress in the gills (keap1, kappa, gsta, gstt1, gstk1, SOD, and CAT) and in the liver (GPx, keap1, kappa, gsta, gstt1, gr, and SOD). In the liver, GPx activity and the content of MDA were significantly upregulated after 7 days of domestication, while in the gills, SOD activity was significantly upregulated after 7 days of domestication and GPx activity was significantly downregulated after 14 days of domestication. These results suggest that artificial feed domestication is associated with oxidative stress. Moreover, these results provide experimental basic data for increasing the production of aquaculture feed for Mandarin fish.

Study of association between corneal shape parameters and axial length elongation during orthokeratology using image-pro plus software

BackgroundThe aim was to validate the correlation between corneal shape parameters and axial length growth (ALG) during orthokeratology using Image-Pro Plus (IPP) 6.0 software.MethodsThis retrospective study used medical records of myopic children aged 8–13 years (n = 104) undergoing orthokeratology. Their corneal topography and axial length were measured at baseline and subsequent follow-ups after lens wear. Corneal shape parameters, including the treatment zone (TZ) area, TZ diameter, TZ fractal dimension, TZ radius ratio, eccentric distance, pupil area, and pupillary peripheral steepened zone(PSZ) area, were measured using IPP software. The impact of corneal shape parameters at 3 months post-orthokeratology visit on 1.5-year ALG was evaluated using multivariate linear regression analysis.ResultsALG exhibited significant associations with age, TZ area, TZ diameter, TZ fractal dimension, and eccentric distance on univariate linear regression analysis. Multivariate regression analysis identified age, TZ area, and eccentric distance as significantly correlated with ALG (all P < 0.01), with eccentric distance showing the strongest correlation (β = −0.370). The regressive equation was y = 1.870 − 0.235a + 0.276b − 0.370c, where y represents ALG, a represents age, b represents TZ area, and c represents eccentric distance; R2 = 0.27). No significant relationships were observed between the TZ radius ratio, pupillary PSZ area, and ALG.ConclusionsIPP software proves effective in capturing precise corneal shape parameters after orthokeratology. Eccentric distance, rather than age or the TZ area, significantly influences ALG retardation.

Effect of corneal e-value on myopia control in children and adolescents with orthokeratology

Objective: To investigate the influence of corneal e-value on the effectiveness of orthokeratology in controlling myopia in children and adolescents. Methods: A retrospective cohort study was conducted, involving the data from 1 563 myopic patients (1 563 eyes) who underwent orthokeratology at the Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine from June 2015 to August 2021 and adhered to lens wear for at least 2 years. The cohort consisted of 737 males and 826 females with an average age of (10.84±2.13) years. Based on corneal e-value parameters obtained from corneal topography, patients were categorized into a low e-value group (n=425) and a high e-value group (n=1 138). Data on gender, age, parental myopia history, and baseline measures such as spherical equivalent (SE), axial length, and corneal e-value were collected. Differences in axial length change and corneal fluorescein staining rates were compared between the two groups at 1 and 2 years after the start of lens wear. A generalized linear mixed model was established with axial length change as the dependent variable to analyze the correlation between axial length change and baseline corneal e-value. Results: The initial age of the 1 563 myopic patients was (10.84±2.13) years, with a baseline SE of (-3.05±1.30) D. After 1 year of lens wear, the axial length change was (0.20±0.19) mm in the low e-value group and (0.24±0.20) mm in the high e-value group. After 2 years, the changes were (0.38±0.25) mm and (0.43±0.27) mm, respectively, with statistically significant differences (all P<0.05). The incidence of corneal staining after 1 year of lens wear was 9.2% (39/425) in the low e-value group and 14.1% (160/1 138) in the high e-value group. After 2 years, the rates were 15.8% (67/425) and 21.8% (248/1 138), respectively, with statistically significant differences (all P<0.05). After adjusting for parental myopia history, age, SE, and baseline axial length, the baseline corneal e-value was positively correlated with axial length change at 1 and 2 years after lens wear (all P<0.05). Conclusions: Corneal e-value is an independent factor influencing the effectiveness of orthokeratology in controlling myopia. A smaller corneal e-value is associated with slower axial length growth after orthokeratology, indicating better control of myopia in treated eyes.

Populus euphratica R2R3-MYB transcription factor RAX2 binds ANN1 promoter to increase cadmium enrichment in Arabidopsis

The expression of R2R3-MYB transcription factor PeRAX2 increased transiently upon CdCl2 exposure (100 μM, 48 h) in leaves and roots of Populus euphratica. We observed that overexpression of PeRAX2 increased Cd2+ concentration in Arabidopsis root cells and Cd2+ amount in whole plant, which was due to the increased Cd2+ influx into root tips. However, the Cd2+ influx facilitated by PeRAX2 overexpression was substantially reduced by LaCl3 (an inhibitor of Ca2+-channels), suggesting that PeRAX2 could promote the Cd2+ entering through PM Ca2+-permeable channels (CaPCs) in the roots. It is noting that the expression of annexin1 (AtANN1), which mediates the influx of divalent cations through the PM calcium channels, was upregulated by Cd2+ in PeRAX2-transgenic Arabidopsis. Bioinformatic analysis revealed that the AtANN1 promoter (AtANN1-pro) contains four cis-elements for MYB binding. The PeRAX2 interaction with AtANN1-pro was validated by LUC reporter assay, EMSA, and Y1H assay. Our data showed that PeRAX2 binds to the AtANN1 promoter region to regulate gene transcription and that AtANN1 mediates the Cd2+ entry through CaPCs in the PM, leading to a Cd2+ enrichment in transgenic plants. The PeRAX2-stimulated Cd2+ enrichment consequently resulted in high H2O2 production in root cells of transgenic plants. The expression of AtSOD and AtPOD and activities of CAT, SOD, POD increased in the transgenic lines under Cd2+ stress. However, the Cd2+-upregulated expression and activity of antioxidative enzymes were less pronounced in the PeRAX2-overexpressed lines, compared to the wildtype and vector controls. As a result, root length and plant growth were more suppressed by Cd2+ in the transgenic lines. Our data suggest that transcriptional regulation of AtANN1 by PeRAX2 can be utilized to improve Cd2+ enrichment and phytoremediation, although the enriched Cd2+ affected antioxidant defense system and plant growth in the model species.

Characterization of a novel fast-growing zebrafish: a new approach to growth hormone transgenesis.

The manipulation of the somatotropic axis, governing growth, has been a focus of numerous transgenic approaches aimed at developing fast-growing fish for research, medicine and aquaculture purposes. However, the excessively high growth hormone (GH) levels in these transgenic fish often result in deformities that impact both fish health and consumer acceptance. In an effort to mitigate these issues and synchronize exogenous GH expression with reproductive processes, we employed a novel transgenic construct driven by a tilapia luteinizing hormone (LH) promoter. This approach was anticipated to induce more localized and lower exogenous GH secretion. In this study, we characterized the growth and reproduction of these transgenic LHp-GH zebrafish using hormonal and physiological parameters. Our findings reveal that LHp-GH fish exhibited accelerated growth in both length and weight, along with a lower feed conversion ratio, indicating more efficient feed utilization, all while maintaining unchanged body proportions. These fish demonstrated higher expression levels of LH and GH in the pituitary and elevated IGF-1 levels in the liver compared to wild-type fish. An examination of reproductive function in LHp-GH fish unveiled lower pituitary LH and FSH contents, smaller follicle diameter in female gonads, and reduced relative fecundity. However, in transgenic males, neither the distribution of spermatogenesis stages nor sperm concentrations differed significantly between the fish lines. These results suggest that coupling exogenous GH expression with endogenous LH expression in females directs resource investment toward somatic growth at the expense of reproductive processes. Consequently, we conclude that incorporating GH under the LH promoter represents a suitable construct for the genetic engineering of commercial fish species, providing accelerated growth while preserving body proportions.