Growth Of Candida Albicans Research Articles

2020. Disinfection of Needle-less Connectors Using UVC Light

Abstract Background Nearly 50% of post-insertion catheter-related infections are due to microbial ingress from needleless connectors (NC), widely used devices which connect to the end of catheters or infusion sets and enable access for injection of medications and attachment of infusions. Infections arising from NC could result in central line associated bloodstream infection (CLABSI). The clinical guideline for disinfecting NC is to wipe the hub of the NC with a 70% alcohol and to manually scrub the connector surface for at least 15 seconds. Unfortunately, multiple studies have found low compliance with the 15 second disinfection scrub. Methods In this study, we investigated the efficacy of 1 second of ultraviolet light-C exposure in the reduction of the 3 most common CLABSI associated organisms: Staphylococcus aureus, Candida albicans, Candida auris, and Methicillin-resistant Staphylococcus aureus (MRSA). We tested a total of 30 NC samples for each organism with 3 positive controls and 1 negative control. Results The log reductions were 5.68, 5.05, 4.41, and 6.38 for S. aureus, C. albicans, C. auris and MRSA, respectively. Growth of Candida albicans with and without UV-C exposure for 1 second. The figure shows the observed growth of Candida albicans from the surface of the connector with and without UV-C exposure for 1 second, displaying no growth on the agar plate for samples that were exposed to 1 second of UV-C disinfection. Log reduction of tested organisms after exposure of UV-C for 1 second. Table 1 displays the overall log reduction of tested organisms after 1 second of UV-C exposure. The results are shown for positive control concentration and log reduction for the test organisms Staphylococcus aureus, Candida albicans, Candida auras, and Methicillin-resistant Staphylococcus aureus. Conclusion Our findings demonstrate greater than 4 log reduction of common CLABSI-associated organisms with 1 second of UV-C exposure, which we believe would decrease CLABSI rates, increase aseptic technique compliance, and reduce disruption in hospital workflow. Disclosures Julia Rasooly, MS, Puracath Medical, Inc: Board Member|Puracath Medical, Inc: Stocks/Bonds Michael Fourkas, MS, Puracath Medical, Inc: Grant/Research Support Gregory Schears, MD, Puracath Medical, Inc: Advisor/Consultant|Puracath Medical, Inc: Stocks/Bonds.

Two distinct lipid transporters together regulate invasive filamentous growth in the human fungal pathogen Candida albicans.

Flippases transport lipids across the membrane bilayer to generate and maintain asymmetry. The human fungal pathogen Candida albicans has 5 flippases, including Drs2, which is critical for filamentous growth and phosphatidylserine (PS) distribution. Furthermore, a drs2 deletion mutant is hypersensitive to the antifungal drug fluconazole and copper ions. We show here that such a flippase mutant also has an altered distribution of phosphatidylinositol 4-phosphate [PI(4)P] and ergosterol. Analyses of additional lipid transporters, i.e. the flippases Dnf1-3, and all the oxysterol binding protein (Osh) family lipid transfer proteins, i.e. Osh2-4 and Osh7, indicate that they are not critical for filamentous growth. However, deletion of Osh4 alone, which exchanges PI(4)P for sterol, in a drs2 mutant can bypass the requirement for this flippase in invasive filamentous growth. In addition, deletion of the lipid phosphatase Sac1, which dephosphorylates PI(4)P, in a drs2 mutant results in a synthetic growth defect, suggesting that Drs2 and Sac1 function in parallel pathways. Together, our results indicate that a balance between the activities of two putative lipid transporters regulates invasive filamentous growth, via PI(4)P. In contrast, deletion of OSH4 in drs2 does not restore growth on fluconazole, nor on papuamide A, a toxin that binds PS in the outer leaflet of the plasma membrane, suggesting that Drs2 has additional role(s) in plasma membrane organization, independent of Osh4. As we show that C. albicans Drs2 localizes to different structures, including the Spitzenkörper, we investigated if a specific localization of Drs2 is critical for different functions, using a synthetic physical interaction approach to restrict/stabilize Drs2 at the Spitzenkörper. Our results suggest that the localization of Drs2 at the plasma membrane is critical for C. albicans growth on fluconazole and papuamide A, but not for invasive filamentous growth.

Development and Evaluation of Ethosomes Loaded with Zingiber zerumbet Linn Rhizome Extract for Antifungal Skin Infection in Deep Layer Skin.

Skin fungal infection is still a serious public health problem due to the high number of cases. Even though medicines are available for this disease, drug resistance among patients has increased. Moreover, access to medicine is restricted in some areas. One of the therapeutic options is herbal medicine. This study aims to develop an ethosome formulation loaded with Zingiber zerumbet (L.) Smith. rhizome extract for enhanced antifungal activity in deep layer skin, which is difficult to cure. Ethosomes were successfully prepared by the cold method, and the optimized formulation was composed of 1% (w/v) phosphatidylcholine and 40% (v/v) ethanol. Transmission electron microscope (TEM) images revealed that the ethosomes had a vesicle shape with a diameter of 205.6-368.5 nm. The entrapment of ethosomes was 31.58% and could inhibit the growth of Candida albicans at a concentration of 312.5 μg/mL. Finally, the ethosome system significantly enhanced the skin penetration and retention of the active compound (zerumbone) compared with the liquid extract. This study showed that Z. zerumbet (L.) rhizome extract could be loaded into ethosomes. The findings could be carried over to the next step for clinical application by conducting further in vivo penetration and permeation tests.

Immunomodulatory drug fingolimod (FTY720) restricts the growth of opportunistic yeast Candida albicans in vitro and in a mouse candidiasis model.

Fingolimod (FTY720) is a drug derived from the fungicidal compound myriocin. As it was unclear whether FTY720 has antifungal effects as well, we aimed to characterize its effect on Candida albicans in vitro and in a mouse candidiasis model. First, antifungal susceptibility testing was performed in vitro. Then, a randomized, six-arm, parallel, open-label trial was conducted on 48 mice receiving oral FTY720 (0.3 mg/kg/day), intraperitoneal C. albicans inoculation, or placebo with different combinations and chorological patterns. The outcome measures of the trial included serum concentrations of interleukin-10 and interferon-gamma, absolute lymphocyte counts, and fungal burden values in the mice's livers, kidneys, and vaginas. Broth microdilution assay revealed FTY720's minimum inhibitory concentration (MIC99) to be 0.25 mg/mL for C. albicans. The infected mice treated with FTY720 showed lower fungal burden values than the ones not treated with FTY720 (p<0.05). As expected, the mice treated with FTY720 showed a less-inflammatory immune profile compared to the ones not treated with FTY720. We hypothesize that FTY720 synergizes the host's innate immune functions by inducing the production of reactive oxygen species. Further studies are warranted to unveil the mechanistic explanations of our observations and clarify further aspects of repurposing FTY720 for clinical antifungal usage.

Comparing the effect of denture adhesive enrichment with three types of chlorhexidine(2%) mouthwash, Matrica (Chamomile extract) and Nystatin on Candida albicans growth: in vitro

Comparing the effect of denture adhesive enrichment with three types of chlorhexidine(2%) mouthwash, Matrica (Chamomile extract) and Nystatin on Candida albicans growth: in vitro

In vitro study of the effect of methanolic and ethanolic extracts of Ziziphora clinopodioides and Biebersteinia multifida on the growth of Streptococcus mutans and Candida albicans

بررسی آزمایشگاهی غلظت مهارکنندگی عصاره‌های متانولی و اتانولی گیاه کاکوتی و قان‌تپر بر رشد باکتری استرپتوکوکوس موتانس و قارچ کاندیدا آلبیکنس

Evaluation of fluconazole, itraconazole, and voriconazole activity on Candida albicans: A case control study.

Azole antifungals are the most commonly used antifungals. The high use of azoles for long-term therapy and prophylaxis is prone to cause resistance. Thus, it is necessary to evaluate the antifungal activity against Candida albicans. Analyzing the comparison of antifungal exposure on the time-kill curve to Candida albicans. A case-control study was conducted with a posttest control group design. This study used Candida albicans clinical and ATCC isolates exposed to antifungal solutions with 1×, 4×, and 16×minimum inhibitory concentrations (MIC). Antibiotics used included fluconazole, itraconazole, and voriconazole. Candida albicans isolates were incubated with MIC, and the number of colonies was counted at 0, 2, 4, 8, 12, 24, and 48h. The number of colonies that grew every hour of observation was included in the time-kill curve. The data were then analyzed using an ANOVA test with p<0.05. The antifungals (fluconazole, itraconazole, and voriconazole) showed fungistatic activity against Candida albicans clinical and ATCC isolates. There was a significant comparison between the antifungal group and the control group at 12, 24, and 48h. The most significant difference between antifungal and control group was found at 24h where fluconazole had 95% CI=0.807-2.061 (p<0.001), itraconazole 95% CI=0.722-1.976 (p<0.001), and voriconazole CI 95%=0.807-2.062 (p<0.001). Fluconazole, itraconazole, and voriconazole were effective in inhibiting the growth of Candida albicans. Maximum inhibition in vitro occurs after 12h of antifungal exposure.

Potential Of Kenikir (Cosmos Caudatus Kunth) Leaves Essential Oil Against Candida Albicans Atcc 10231 In Vitro

BACKGROUND : Kenikir is a medicinal plant whose leaves are often consumed as vegetables. Kenikir leaves contain active compounds such as flavonoids, polyphenols, saponins, tannins, alkaloids and essential oils. These compounds are thought to be able to inhibit the growth of Mycobacterium tuberculosa and Candida albicans ATCC 10231 strains OBJECTIVE : The purpose of this study was to describe the effect of kenikir leaf essential oil on the growth of the Candida albicans ATCC 10231 strains. with 4 concentrations and 6 repetitions in Candida albicans and the concentration of kenikir leaf essential oil concentration of 0.5%, 1%, 1.5%, 2%, METHOD : This type of research is a pure experimental research by providing treatment in the form of giving essential oil of kenikir leaves with various concentrations on the growth of the Candida albicans ATCC 10231 strains. The inhibition test of kenikir leaf essential oil on the growth of the Candida albicans ATCC 10231 strains was carried out using the Kirby Bauer disk diffusion method. The research design used is Post Test Only with Control Group Design.Data obtained in the form of inhibition zone diameter, were analyzed using Variant Analysis (Anova), P£ 0,05 and continued with Post Hoc test. RESULT : The results showed that 0.5% essential oil concentration had a inhibition zone of 9.67 mm (moderate criteria); essential oil concentration of 1% with a inhibition zone of 9.72 mm with criteria moderate and 1.5% concentration with inhibition zone 11.86% with strong criteria and concentration 2% with inhibition zone 12.67 mm with strong criteria all concentrations affect the growth of Candida albicans with the most optimal concentration of 2% for the Candida albicans ATCC 10231 strains. CONCLUSION : The higher the concentration of kenikir leaf extract, the inhibitory effect on the growth of Candida albicans ATCC 10231 strains was also higher. KEYWORDS : Cosmos caudatus Kunth, Candida albicans ATCC 10231 strains, diameter of the inhibition zone

Comparison of The Effectiveness of Cleaning Products Herbal Ingredients Against the Growth of Candida albicans

Leukorrhea is a symptom that arises from vaginitis. This disease is mostly caused by Candida infections, especially Candida albicans. This disease causes an uncomfortable feeling for the sufferer. for that, we need to maintain the balance of the vaginal ecosystem. The aim of the research was to find out the Comparison of the Effectiveness of the Inhibitory Power of Feminine Cleansing Products Made from Herbs on the Growth of Candida albicans. This type of research was carried out experimentally, which is a laboratory study using a simple experimental design. The research was conducted in October 2022 at the Microbiology Laboratory of the Pharmacy Department of the Sandi Karsa Polytechnic Makassar. Based on the results of the research conducted, it can be concluded that the effectiveness of the inhibition of feminine products in Sample I was stronger in inhibiting the growth of Candida albicans compared to Sample II.

Azobenzene derivatives with activity against drug-resistant Candida albicans and Candida auris.

Increasing resistance against antimycotic drugs challenges anti-infective therapies today and contributes to the mortality of infections by drug-resistant Candida species and strains. Therefore, novel antifungal agents are needed. A promising approach in developing new drugs is using naturally occurring molecules as lead structures. In this work, 4,4'-dihydroxyazobenzene, a compound structurally related to antifungal stilbene derivatives and present in Agaricus xanthodermus (yellow stainer), served as a starting point for the synthesis of five azobenzene derivatives. These compounds prevented the growth of both fluconazole-susceptible and fluconazole-resistant Candida albicans and Candida auris strains. Further in vivo studies are required to confirm the potential therapeutic value of these compounds.

Uji Daya Hambat Ekstrak Cabai Hijau (Capsicum annum L) Terhadap Pertumbuhan Jamur Candida albicans Secara In Vitro

Fungal infection is one of the diseases that has experienced an increasing incidence in the world, especially Indonesia with its geographical condition as a country with a tropical climate with high temperature and humidity. One of the most common fungal infections is caused by the fungus Candida albicans. The incidence of antifungal resistance has become a health problem and has caused an increase in mortality. Efforts must be made to overcome this problem by utilizing secondary metabolite compounds found in green chili plants. Green chili (Capsicum annum L) contains secondary metabolites such as flavonoids, saponins, tannins, triterpenes/steroids and glycosides which act as antifungals. The purpose of this study was to test whether the extract of green chili (Capsicum annum L) could inhibit the growth of the fungus Candida albicans. The research method used is the posttest only control group design method. The effect of green chili extract was tested by diffusion method with concentrations of 2%, 4%, and 6% with a positive control of ketoconazole and a negative control of Dimethylsulfoxide (DMSO). Each treatment was carried out five times. The diameter of the inhibition zone was measured with a caliper. The results showed inhibition at all concentrations, namely 2% (7.9mm), 4% (10.6mm), 6% (11.2mm). The conclusion of this study was that the extract of green chili (Capsicum annum L) had the highest inhibitory power at a concentration of 6%.

Uji Daya Hambat Ekstrak Cabai Hijau (Capsicum annum L) Terhadap Pertumbuhan Jamur Candida albicans Secara In Vitro

Fungal infection is one of the diseases that has experienced an increasing incidence in the world, especially Indonesia with its geographical condition as a country with a tropical climate with high temperature and humidity. One of the most common fungal infections is caused by the fungus Candida albicans. The incidence of antifungal resistance has become a health problem and has caused an increase in mortality. Efforts must be made to overcome this problem by utilizing secondary metabolite compounds found in green chili plants. Green chili (Capsicum annum L) contains secondary metabolites such as flavonoids, saponins, tannins, triterpenes/steroids and glycosides which act as antifungals. The purpose of this study was to test whether the extract of green chili (Capsicum annum L) could inhibit the growth of the fungus Candida albicans. The research method used is the posttest only control group design method. The effect of green chili extract was tested by diffusion method with concentrations of 2%, 4%, and 6% with a positive control of ketoconazole and a negative control of Dimethylsulfoxide (DMSO). Each treatment was carried out five times. The diameter of the inhibition zone was measured with a caliper. The results showed inhibition at all concentrations, namely 2% (7.9mm), 4% (10.6mm), 6% (11.2mm). The conclusion of this study was that the extract of green chili (Capsicum annum L) had the highest inhibitory power at a concentration of 6%.

Amphotericin B- and Levofloxacin-Loaded Chitosan Films for Potential Use in Antimicrobial Wound Dressings: Analytical Method Development and Its Application.

Levofloxacin (LVX) and amphotericin B (AMB) have been widely used to treat bacterial and fungal infections in the clinic. Herein, we report, for the first time, chitosan films loaded with AMB and LVX as wound dressings to combat antimicrobial infections. Additionally, we developed and validated a high-performance liquid chromatography (HPLC) method coupled with a UV detector to simultaneously quantify both AMB and LVX. The method is easy, precise, accurate and linear for both drugs at a concentration range of 0.7-5 µg/mL. The validated method was used to analyse the drug release, ex vivo deposition and permeation from the chitosan films. LVX was released completely from the chitosan film after a week, while approximately 60% of the AMB was released. Ex vivo deposition study revealed that, after 24-hour application, 20.96 ± 13.54 µg of LVX and approximately 0.35 ± 0.04 µg of AMB was deposited in porcine skin. Approximately 0.58 ± 0.16 µg of LVX permeated through the skin. AMB was undetectable in the receptor compartment due to its poor solubility and permeability. Furthermore, chitosan films loaded with AMB and LVX were found to be able to inhibit the growth of both Candida albicans and Staphylococcus aureus, indicating their potential for antimicrobial applications.

Genetic Engineering of Talaromyces marneffei to Enhance Siderophore Production and Preliminary Testing for Medical Application Potential.

Siderophores are compounds with low molecular weight with a high affinity and specificity for ferric iron, which is produced by bacteria and fungi. Fungal siderophores have been characterized and their feasibility for clinical applications has been investigated. Fungi may be limited in slow growth and low siderophore production; however, they have advantages of high diversity and affinity. Hence, the purpose of this study was to generate a genetically modified strain in Talaromyces marneffei that enhanced siderophore production and to identify the characteristics of siderophore to guide its medical application. SreA is a transcription factor that negatively controls iron acquisition mechanisms. Therefore, we deleted the sreA gene to enhance the siderophore production and found that the null mutant of sreA (ΔsreA) produced a high amount of extracellular siderophores. The produced siderophore was characterized using HPLC-MS, HPLC-DAD, FTIR, and 1H- and 13C-NMR techniques and identified as a coprogen B. The compound showed a powerful iron-binding activity and could reduce labile iron pool levels in iron-loaded hepatocellular carcinoma (Huh7) cells. In addition, the coprogen B showed no toxicity to the Huh7 cells, demonstrating its potential to serve as an ideal iron chelator. Moreover, it inhibits the growth of Candida albicans and Escherichia coli in a dose-dependent manner. Thus, we have generated the siderophore-enhancing strain of T. marneffei, and the coprogen B isolated from this strain could be useful in the development of a new iron-chelating agent or other medical applications.

The inhibitory effect of watery extracts of Glycyrrhiza Glabra roots on the growth of Candida albicans in comparison with antifungal antibiotics

This study was made to evaluate the watery extract both hot and cold of Glycyrrhiza Glabra root on the growing of Candida albicans comparing with Nystatin and Clotrimazol .both cold and hot extract shows inhibitory effect with significant value on (P&lt;0.05) on the growing of Candida albicans comparing with distal water and Nystatin in which the yeast was resistant to that drug . The hot extract in concentration (200 mg/ml) more effective than Clotrimazole , while the cold watery extract did not show the same effect . The hot watery extract was more effective than the cold one .The average of inhibition zone for the first extract on the concentration 50,100,150,200 mg/ml were 11.2,14.6,17.1,21.5 mm, while the average of inhibition zone for the second extract at the same concentrations as following 9.3,21.1,16.6,17.7 mm ,the results shows that the watery extract of Glycyrrhiza Glabra root can be use as an antifungal in the infection with Candida albicans

Test of The Inhibitory Power of Lemongrass Extract Against The Growth of Candida albicans On Natural Resources Medium

Candidiasis is an acute or chronic inflammation that causes disorders of the body's system caused by the fungus Candida albicans. Candida albicans is a common cause that does not cause disease in a person's normal immune system but can attack a person's poor system. Candidiasis can be treated with antifungal drugs, but prolonged and extensive use of antifungal drugs causes side effects. So alternative drugs are needed that can reduce these side effects. Lemongrass (Cymbopogon citratus) has pharmacological activity as an antifungal. Research Objectives: To determine the inhibition of lemongrass extract on the growth of Candida albicans in an SDA medium with concentrations of 0%, 0.2%, 0.4%, 0.6%, 0.8%, and 1%. The study used a descriptive research design. The method of testing the resistance is carried out by the good diffusion method. Lemongrass extract can inhibit the growth of Candida albicans fungus by forming an inhibition zone. At a concentration of 0% lemongrass extract, no inhibition zone was formed, a concentration of 0.2% formed an inhibitory zone of 10.5 mm, a concentration of 0.4% formed an inhibition of 6.1 mm, a concentration of 0.6% formed an inhibition zone of 14 mm, the concentration 0.8% formed an inhibition zone of 10.8 mm, a concentration of 15 formed an inhibitory zone of 27.6 mm.

PRODUK BIOTEKNOLOGI FARMASI BERUPA SABUN MANDI KOMBUCHA BUNGA TELANG (Clitoria ternatea L) SEBAGAI ANTIFUNGI Candida albicans

Telang flower kombucha besides playing an important role in the food sector as a probiotic drink, it has potential in the pharmaceutical (cosmetic) field, especially in the preparation of probiotic liquid bath soap in inhibiting the growth of Candida albicans which causes vaginal discharge in the female intimate organs. This study aims to make formulations and preparations of probiotic liquid bath soap with active ingredients from fermented kombucha telang flower solution at concentrations of 20%, 30%, and 40% in inhibiting the growth of Candida albicans. This research method is a laboratory experiment by making 4 preparations of probiotic liquid bath soap with active ingredients from fermented kombucha telang flower solution at concentrations of 20%, 30%, 40%, and soap base as a negative control and the use of biore soap available in the market as a positive control. Testing on the growth inhibition of Candida albicans using the disc diffusion method. Data analysis was carried out statistically using one-way ANOVA and further tests were carried out in the form of post hoc analysis. The conclusion in this study was that the concentration of 40% in the preparation of kombucha flower telang bath soap was the best concentration as antifungal Candida albicans with an average diameter of the resulting inhibition zone was 20.52 mm.

Uji Daya Hambat Ekstrak Lengkuas (Alpinia galanga) Terhadap Pertumbuhan Jamur Candida albicans

Galangal (Alpinia galanga) is a nutritious plant that can cure various diseases. This part of the plant, such as the rhizome, functions as a traditional medicine. This study aims to determine the inhibition of the fungus Candida albicans using galangal extract. Galangal extract was made using three different kinds of the solvents, namely hexane, ethyl acetate, and methanol. The suspension of Candida albicans fungus was obtained from the Biology Laboratory, FMIPA, Tadulako University. Fungal test using the well method. The results showed that the inhibitory power of galangal extract against the growth of Candida albicans using ethyl acetate and methanol was greater than that of hexane. Based on observations, the percentage of inhibitory power of galangal extract on the growth of Candida albicans with hexane, ethyl acetate, and methanol as solvents was 0% for hexane, 1,70% for ethyl acetate, and 4,70% for methanol.

Antibiofilm and Antivirulence Activities of Gold and Zinc Oxide Nanoparticles Synthesized from Kimchi-Isolated Leuconostoc sp. Strain C2.

The rapid emergence of antimicrobial resistance (AMR) among bacterial pathogens results in antimicrobial treatment failure and the high mortality rate associated with AMR. The application of nanoparticles synthesized from probiotics will be widely accepted due to their efficacy and biocompatibility in treating microbial infections in humans. The current work sought to isolate and identify lactic acid bacteria (LAB) from Kimchi. Based on 16S rRNA gene sequencing, the LAB isolate C2 was identified as a member of the genus Leuconostoc. The obtained supernatant from Leuconostoc sp. strain C2 was employed for the green synthesis of metal (AuNPs) and metal oxide (ZnONPs) nanoparticles. UV-vis absorption spectra, FTIR analysis, XRD, DLS, FE-TEM, and EDS mapping were used to fully characterize these C2-AuNPs and C2-ZnONPs. The C2-AuNPs were found to be spherical in shape, with a size of 47.77 ± 5.7 nm and zeta potential of -19.35 ± 0.67 mV. The C2-ZnONPs were observed to be rod-shaped and 173.77 ± 14.53 nm in size. The C2-ZnONPs zeta potential was determined to be 26.62 ± 0.35 mV. The C2-AuNPs and C2-ZnONPs were shown to have antimicrobial activity against different pathogens. Furthermore, these nanoparticles inhibited the growth of Candida albicans. The antibiofilm and antivirulence properties of these NPs against Pseudomonas aeruginosa and Staphylococcus aureus were thoroughly investigated. C2-AuNPs were reported to be antibiofilm and antivirulence against P. aeruginosa, whereas C2-ZnONPs were antibiofilm and antivirulence against both P. aeruginosa and S. aureus. Furthermore, these nanoparticles disrupted the preformed mature biofilm of P. aeruginosa and S. aureus. The inhibitory impact was discovered to be concentration-dependent. The current research demonstrated that C2-AuNPs and C2-ZnONPs exhibited potential inhibitory effects on the biofilm and virulence features of bacterial pathogens. Further studies are needed to unravel the molecular mechanism behind biofilm inhibition and virulence attenuation.

GAMBARAN Candida albicanss PADA URIN REMAJA DI PANTI ASUHAN X BANJARMASIN

Candida albicans is one of the most pathogenic species and often causes vaginitis in women with the main symptom of vaginal discharge. As many as 75% of women in Indonesia have experienced vaginal discharge at least once in their life and half of them have experienced vaginal discharge twice or more. This study aims to calculate the percentage of Candida albicanss in the urine of adolescents at the Putri Orphanage in Banjarmasin. This type of research is quantitative and descriptive with a cross-sectional design. The research sample was 21 urine sample teenagers at the Orphanage in Banjarmasin. The sampling technique used was purposive sampling. Laboratory examinations included macroscopic observations of Candida colonies growing on Sabouraud Dextrose Agar (SDA) media, and microscopic observations using the Germ Tube test to distinguish Candida albicanss species from Candida non-albicanss. And by filling out a questionnaire to determine the level of knowledge about vaginal discharge and personal hygiene. The results showed 33% of respondents were positive for Candida albicanss. Based on the results, overview Candida albicans in adolescents at the Putri Asri Orphanage in Banjarmasin, it can be concluded that there are 7 samples (33%) positive for Candida albicans. Personal hygiene and environmental sanitation can affect the growth of Candida albicans which can cause vaginal discharge in adolescent girls.