Wheat Growth Research Articles

Effects of seeding methods and nutrient management practices on growth and yield of organic wheat (Triticum aestivum)

An experiment was conducted out during winter (rabi) seasons of 2021–22 and 2022–23 at the Research Farm of Maharana Pratap Univesity of Agriculture and Technology, Udaipur, Rajasthan to study the effect of nutrient management practices and sowing methods on the development and production of organic wheat (Triticum aestivum L.). The experiment was conducted in a split plot design (SPD) with 4 replications. The experiment consisted of 3 sowing methods in main plots, viz. Flat row sowing; FIRB (Furrow Irrigated Raised Bed) sowing; and zero tillage sowing and 8 nutrient management practices, viz. 100% RDN through FYM as basal; RDN through FYM as basal + 50% RDN with first irrigation; 75% RDN through FYM as basal + 25% RDN with first irrigation; 50% RDN through FYM as basal + 25% RDN with first irrigation + 25% RDN with second irrigation; 100% RDN through FYM as basal + jeevamrit @500 litre/ha at sowing and first irrigation + panchagavya @5% at booting stage; 50% RDN through FYM as basal + 50% RDN with first irrigation + jeevamrit @500 litre/ha at sowing and first irrigation + panchagavya @5% at booting stage; 75% RDN through FYM as basal + 25% RDN with first irrigation + jeevamrit @500 litre/ha at sowing and first irrigation + panchagavya @5% at booting stage; 50% RDN through FYM as basal + 25% RDN with first irrigation + 25% RDN with second irrigation + jeevamrit @500 litre/ha at sowing and first irrigation + panchagavya @5% at booting stage in sub plots. The experimental results revealed that the FIRB sowing method recorded significantly higher plant height at 45 DAS (42.56 and 42.66 cm) during both the years of study over zero tillage sowing method and remain at par with flat row sowing method, respectively. The same sowing method resulted into statistically higher grain yield (42.88 and 43.87 q/ha) and biological yield (106.89 and 108.48 q/ha) compared to flat row sowing and zero tillage sowing methods during both the years, respectively. During the second year of study (2023) significantly highest crop growth rate (CGR) (1.1240 g/m2/day) and relative growth rate (RGR) (0.0083 g/g/day) between 90 DAS to harvest were recorded with flat row sowing over zero tillage sowing and remain at par with FIRB sowing method. Regarding the nutrient management practices the highest plant height (43.43 and 44.51 cm), grain yield (44.52 and 44.84 q/ha) and biological yield (110.35 and 110.58 q/ha) were recorded with 75% RDN through FYM as basal + 25% RDN with first irrigation + jeevamrit @500 litre/ha at sowing and first irrigation + panchagavya @5% at booting stage.

Stress-Responsive Gene Expression, Metabolic, Physiological, and Agronomic Responses by Consortium Nano-Silica with Trichoderma against Drought Stress in Bread Wheat

The exploitation of drought is a critical worldwide challenge that influences wheat growth and productivity. This study aimed to investigate a synergistic amendment strategy for drought using the single and combined application of plant growth-promoting microorganisms (PGPM) (Trichoderma harzianum) and biogenic silica nanoparticles (SiO2NPs) from rice husk ash (RHA) on Saudi Arabia’s Spring wheat Summit cultivar (Triticum aestivum L.) for 102 DAS (days after sowing). The significant improvement was due to the application of 600 ppm SiO2NPs and T. harzianum + 600 ppm SiO2NPs, which enhanced the physiological properties of chlorophyll a, carotenoids, total pigments, osmolytes, and antioxidant contents of drought-stressed wheat plants as adaptive strategies. The results suggest that the expression of the studied genes (TaP5CS1, TaZFP34, TaWRKY1, TaMPK3, TaLEA, and the wheat housekeeping gene TaActin) in wheat remarkably enhanced wheat tolerance to drought stress. We discovered that the genes and metabolites involved significantly contributed to defense responses, making them potential targets for assessing drought tolerance levels. The drought tolerance indices of wheat were revealed by the mean productivity (MP), stress sensitivity index (SSI), yield stability index (YSI), and stress tolerance index (STI). We employed four databases, such as BAR, InterPro, phytozome, and the KEGG pathway, to predict and decipher the putative domains in prior gene sequencing. As a result, we discovered that these genes may be involved in a range of important biological functions in specific tissues at different developmental stages, including response to drought stress, proline accumulation, plant growth and development, and defense response. In conclusion, the sole and/or dual T. harzianum application to the wheat cultivar improved drought tolerance strength. These findings could be insightful data for wheat production in Saudi Arabia under various water regimes.

The role of organic amendments combined with nitrogen fertilizers in enhancing nitrogen use efficiency for wheat in saline calcareous soil

This study investigates the effects of combining different nitrogen fertilizers with organic amendments and Azospirillum brasilense on wheat growth, yield, and nutrient uptake in saline calcareous soil, with a goal of enhancing crop productivity while reducing environmental impacts. The objective was to evaluate treatments involving ammonium sulfate (AS), urea, and sulfur-coated urea (SCU), mixed with organic amendments such as Moringa seed residues (MSR), biogas manure (BM), and vermicompost (Ver), along with Azospirillum brasilense (ASB). Among these treatments, SCU emerged as the most effective nitrogen source, especially when combined with Vermicompost and ASB. Key results include the following: the highest Chlorophyll a content of 1.96 mg/g f wt was observed in the SCU treatment with Ver and ASB; Likewise, the same treatment exhibited the highest values of Chlorophyll b, carotenoids, and proline; the tallest plant height (117.50 cm) was also achieved with this combination; and the highest straw and grain weights (2.63 g/plant and 2.22 g/plant, respectively) came from the same treatment. Additionally, this treatment yielded the highest protein content at 19.29%. In terms of nutrient uptake, SCU-based treatments consistently demonstrated the highest values. The SCU with Ver + ASB treatment recorded the highest nitrogen uptake in straw at 78.64 mg plant−1, phosphorus uptake at 12.86 mg plant−1, and potassium uptake at 61.66 mg plant−1. Similarly, this combination yielded the highest N, P, and K uptake in grains, with 68.05, 10.88, and 53.15 mg plant-1, respectively. e

YOLOv5s-Based Image Identification of Stripe Rust and Leaf Rust on Wheat at Different Growth Stages.

Stripe rust caused by Puccinia striiformis f. sp. tritici and leaf rust caused by Puccinia triticina, are two devastating diseases on wheat, which seriously affect the production safety of wheat. Timely detection and identification of the two diseases are essential for taking effective disease management measures to reduce wheat yield losses. To realize the accurate identification of wheat stripe rust and wheat leaf rust during the different growth stages, in this study, the image-based identification of wheat stripe rust and wheat leaf rust during different growth stages was investigated based on deep learning using image processing technology. Based on the YOLOv5s model, we built identification models of wheat stripe rust and wheat leaf rust during the seedling stage, stem elongation stage, booting stage, inflorescence emergence stage, anthesis stage, milk development stage, and all the growth stages. The models were tested on the different testing sets in the different individual growth stages and in all the growth stages. The results showed that the models performed differently in disease image identification. The model based on the disease images acquired during an individual growth stage was not suitable for the identification of the disease images acquired during the other individual growth stages, except for the model based on the disease images acquired during the milk development stage, which had acceptable identification performance on the testing sets in the anthesis stage and the milk development stage. In addition, the results demonstrated that wheat growth stages had a great influence on the image identification of the two diseases. The model built based on the disease images acquired in all the growth stages produced acceptable identification results. Mean F1 Score values between 64.06% and 79.98% and mean average precision (mAP) values between 66.55% and 82.80% were achieved on each testing set composed of the disease images acquired during an individual growth stage and on the testing set composed of the disease images acquired during all the growth stages. This study provides a basis for the image-based identification of wheat stripe rust and wheat leaf rust during the different growth stages, and it provides a reference for the accurate identification of other plant diseases.

Impact of Stage-Specific Irrigation on the Growth and Yield of Wheat

Stage-specific irrigation is essential for ensuring optimal water use at critical growth stages in wheat (Triticum aestivum), leading to increased yield and improved grain quality. Therefore, determining an appropriate irrigation schedule for wheat is crucial for enhancing productivity. Keeping this view in mind, an experiment was conducted at the farmer’s fields of Dinajpur Sadar Upazila, Dinajpur, Bangladesh from November 2020 to April 2021, aimed to evaluate the impact of irrigation schedules on the growth and yield of wheat (BARI Gom-33). A randomized complete block design (RCBD) with seven treatments and three replications was implemented. Treatments included varying irrigation timings: T0 (no irrigation), T1 (one irrigation at maximum tillering), T2 (one irrigation at booting), T3 (one irrigation at heading), T4 (two irrigations at maximum tillering and heading), T5 (milking dough), and T6(two irrigations at booting and milking dough). Key metrics assessed included plant height, leaf count, tiller number, dry matter, growth stages, grain number, thousand grain weight, and overall yield at different days’ intervals. Results indicated that irrigated treatments (T1-T6) significantly improved the parameters for growth and yield compared to the control (T0). Notably, T4, with two irrigations, yielded the highest grain production at 5.20 t/ha, followed closely by T6. These findings highlight the significance of proper irrigation scheduling for optimizing wheat yield, indicating that applying two irrigations at the maximum tillering and heading stages can significantly enhance agricultural productivity.

Genome-Wide Characterization and Expression Profiling of Phytosulfokine Receptor Genes (PSKRs) in Triticum aestivum with Docking Simulations of Their Interactions with Phytosulfokine (PSK): A Bioinformatics Study.

Background/Objectives: The phytosulfokine receptor (PSKR) gene family plays a crucial role in regulating plant growth, development, and stress response. Here, the PSKR gene family was characterized in Triticum aestivum L. The study aimed to bridge knowledge gaps and clarify the functional roles of TaPSKRs to create a solid foundation for examining the structure, functions, and regulatory aspects. Methods: The investigation involved genome-wide identification of PSKRs through collection and chromosomal assignment, followed by phylogenetic analysis and gene expression profiling. Additionally, interactions with their interactors were stimulated and analyzed to elucidate their function. Results: The wide-genome inspection of all TaPSKRs led to 25 genes with various homeologs, resulting in 57 TaPSKR members distributed among the A, B, and D subgenomes. Investigating the expression of 61 TaPSKR cDNAs in RNA-seq datasets generated from different growth stages at 14, 21, and 60 days old and diverse tissues such as leaves, shoots, and roots provided further insight into their functional purposes. The expression profile of the TaPSKRs resulted in three key clusters. Gene cluster 1 (GC 1) is partially associated with root growth, suggesting that specific TaPSKRs control root development. The GC 2 cluster targeted genes that show high levels of expression in all tested leaf growth stages and the early developmental stage of the shoots and roots. Furthermore, the GC 3 cluster was composed of genes that are constantly expressed, highlighting their crucial role in regulating various processes during the entire life cycle of wheat. Molecular docking simulations showed that phytosulfokine type α (PSK-α) interacted with all TaPSKRs and had a strong binding affinity with certain TaPSKR proteins, encompassing TaPSKR1A, TaPSKR3B, and TaPSKR13A, that support their involvement in PSK signaling pathways. The crucial arbitration of the affinity may depend on interactions between wheat PSK-α and PSKRs, especially in the LRR domain region. Conclusions: These discoveries deepened our knowledge of the role of the TaPSKR gene family in wheat growth and development, opening up possibilities for further studies to enhance wheat durability and yield via focused innovation approaches.

Cadmium immobilization by mercapto-palygorskite in alkaline soil: Impacts on soil microbial communities and wheat rhizosphere metabolism

Weakly alkaline cadmium (Cd) contaminated soil in China has aroused great concern regarding its impact on food security and human health. Mercapto-modified palygorskite (MP) has exhibited good potential to minimize Cd accumulation in wheat, it is imperative to understand the underlying mechanisms within the soil-wheat-microbial system for sustainable development of agrochemicals. This study evaluated the effects of various MP dosages on soil Cd bioavailability, rhizosphere metabolomics, microbial community structure and wheat growth. The results indicated that MP (0.05–0.2 %) application significantly reduced Cd accumulation in wheat grains by 59.0–83.2 % (p < 0.05) and inhibited Cd translocation from root to grain. MP also promoted Mn oxide formation and redistributed the exchangeable Cd to Fe–Mn oxide-bound forms (44.2–109.6 %), thus lowering soil Cd bioavailability by 17.9–32.5 %. Additionally, MP reduced wheat rhizosphere organic acid levels, altered rhizosphere carbon and nitrogen pools, and stimulated the growth of Cd-tolerant Alternaria and Cladosporium, while inhibiting the growth of Fusarium. These findings highlight the potential of MP to modulate soil rhizosphere metabolism and microbial communities, offering a novel perspective on its environmental implications and supporting agrochemical sustainability.

Application of different phosphatic fertilizers influences the different phosphorus fractions and morphophysiological traits of wheat in saline sodic soil

Nutrient constraints in salt-affected soils are one of the limiting factors for crop production. We investigated phosphorus (P) availability to wheat crop and its correlations with different fractions of P in salt-affected soils in response to various phosphatic fertilizer’s application. Three different types of soils having electrical conductivity of soil extract (ECe) < 4 dS m−1 and sodium adsorption ratio (SAR) < 13.2 (mmol L−1)1/2, ECe 8 dS m−1 SAR 20 (mmol L−1)1/2 and ECe 12 dS m−1 and SAR 30 (mmol L−1)1/2 were used for this experiment. Different P fertilizers viz. control, diammonium phosphate (DAP), single super phosphate (SSP) and nitrophos (NP) were applied on these soils. The doses of nitrogen, phosphorus and potassium were remained same for all the treatments. The P fractions, agronomic parameters and P-availability to plants were determined. Results indicated increase in shoot dry weight (61%) and photosynthetic rate (9%) in SSP treated soil at EC 8 dS m−1 and SAR 20 (mmol L)1/2. The total P (7.6%) in soil and respiration rate (1.9%) in plant was recorded maximum in DAP treated soil at EC 8 dS m−1 and SAR 20 (mmol L)1/2, and membrane stability index (44%) in plant shoot, extractable P (23%) in soil and P concentration (22%) in shoot were recorded maximum in SSP treated soil at EC 12 dS m−1 and SAR 30 (mmol L)1/2. It was concluded that SSP fertilizer perform better in salt-affected soil for growth and yield of wheat compared to other phosphatic fertilizers.

Bacillus subtilis Strain YJ-15, Isolated from the Rhizosphere of Wheat Grown under Saline Conditions, Increases Soil Fertility and Modifies Microbial Community Structure

Soil salinization during wheat cultivation considerably diminishes soil fertility and impedes wheat growth, primarily due to rhizosphere microbial community changes. Our study investigates the application of Bacillus subtilis YJ-15, a strain isolated from the rhizosphere of wheat cultivated in salinized soil, as a soil remediation agent. This strain has demonstrated significant salt tolerance, disease suppression capabilities, and growth-promoting attributes in previous studies. The wheat rhizosphere was examined to assess the impact of Bacillus subtilis YJ-15 on microbial community composition and soil fertility. Fertility of soil in saline soil was significantly increased by inoculating wheat with YJ-15. The microbial community structure within the wheat rhizosphere inoculated with Bacillus subtilis YJ-15 was analyzed through sequencing on the Illumina MiSeq platform. Phyla Proteobacteria and Acidobacteria were identified as the dominant bacteria. Basidiomycota, Mortierellomycota, and Ascomycota dominated the fungal phyla. Among the bacterial genera, Pseudomonas, Arthrobacter, and Bacillus were predominant. The predominant fungal genera included Alternaria, Cephalotrichum, Mortierella, and Chaetomium. A significant increase in Gaiella and Haliangium levels was observed in the YJ group compared to the control group. Additionally, the fungal genera Epicoccum, Sporidiobolus, and Lecythophora have significantly increased in YJ abundance. One of the potential benefits of Bacillus subtilis YJ-15 in the cultivation of wheat on salinized land is its ability to enhance the rhizosphere microbial community structure and improve soil fertility.

TaNAR2.1 and TaNAR2.2 differ in influencing nitrogen uptake and growth of wheat (Triticum aestivum L.)

NAR2 (Nitrate assimilation related protein) is a protein chaperone involved in transporting nitrate across membranes. However, the expression pattern and function of NAR2 genes in wheat are still largely unknown. Here, we cloned two TaNAR2 genes (TaNAR2.1 and TaNAR2.2). To assess and compare the functional differences of TaNAR2.1 and TaNAR2.2, we analyzed the subcellular localization and expression pattern of the two genes in wheat under low nitrogen (LN) and high nitrogen (HN) conditions, as well as the nitrate influx and root system architecture of TaNAR2.1 and TaNAR2.2 overexpression wheat under LN and HN. Additionally, we investigated the effects of TaNAR2.1 and TaNAR2.2 overexpression on the growth phenotype, nitrogen uptake and yield of wheat throughout the growth period. There are significant differences in the expression patterns and functions of TaNAR2.1 and TaNAR2.2. TaNAR2.1 is located in the cytoplasm, nucleus and the plasma membrane, whereas TaNAR2.2 is a cytoplasm-specific protein. TaNAR2.1 appears to exhibit larger changes in expression levels and a higher capacity for nitrate influx than TaNAR2.2 under external nitrate supply. Overexpression of TaNAR2.1 significantly improves grain nitrogen use efficiency and increases grain yield, whereas overexpression of TaNAR2.2 enhances vegetative and reproductive growth of wheat roots. These findings indicate that TaNAR2.1 plays a crucial role in wheat nitrogen accumulation and yield, while TaNAR2.2 is pivotal for wheat root growth.

Effects of phosphate-solubilizing bacteria Aspergillus flavus AF-LRH1 on promoting phosphorus solubilization, wheat growth and soil heavy metal remediation

Increasing nutrient supply level and eliminating pollution risk are essential to promote farmland soil use. In the soil-plant nutrition cycle, phosphorus is a limiting nutrient, toxic metals such as Cd and Pb are stress threats. Utilizing microorganisms as a bio-amendment could be a viable approach to enhance the cycling of soil phosphorus nutrients and immobilize Cd and Pb in the soil. This study involved the isolation of Aspergillus flavus AF-LRH1, a phosphate solubilizing fungal strain from soil. The strain exhibited the capacity to solubilize calcium phosphate, attaining a phosphorus concentration of 220.73 mg L−1 in the medium. High performance liquid chromatography analysis showed that AF-LRH1 produced a combination of organic acids (tartaric acid 260.7 mg L−1, fumaric acid 0.8 mg L−1, citric acid 911.9 mg L−1) to dissolve Ca3(PO4)2 by providing H+ ions. AF-LRH1 also utilizes NH4+ for the biosynthesis of amino acids and releases protons, which facilitate the dissolution of insoluble Ca3(PO4)2 and calcium phytate. Through these processes, calcium phosphate is partly converted into hydroxyapatite because of biological solubilization. AF-LRH1 strain showed the capability to recover phosphorus from various types of sludge-based solid residue by increasing the solubility of insoluble phosphate compounds for plant nutrient supply. In addition, the combining addition of AF-LRH1 strain and phosphate-containing amendments (calcium phosphate, sludge ash and biochar) benefited the decontamination of Pb and Cd contaminated soil. This finding confirmed the AF-LRH1 could be a promising environmental-friendly biofertilizer suitable for agricultural applications and bioremediation of heavy metal contaminated soil.

UAV-Based Phenotyping: A Non-Destructive Approach to Studying Wheat Growth Patterns for Crop Improvement and Breeding Programs

Rising food demands require new techniques to achieve higher genetic gains for crop production, especially in regions where climate can negatively affect agriculture. Wheat is a staple crop that often encounters this challenge, and ideotype breeding with optimized canopy traits for grain yield, such as determinate tillering, synchronized flowering, and stay-green (SG), can potentially improve yield under terminal drought conditions. Among these traits, SG has emerged as a key factor for improving grain quality and yield by prolonging photosynthetic activity during reproductive stages. This study aims to highlight the importance of growth dynamics in a wheat mapping population by using multispectral images obtained from uncrewed aerial vehicles as a high-throughput phenotyping technique to assess the effectiveness of using such images for determining correlations between vegetation indices and grain yield, particularly regarding the SG trait. Results show that the determinate group exhibited a positive correlation between NDVI and grain yield, indicating the effectiveness of these traits in yield improvement. In contrast, the indeterminate group, characterized by excessive vegetative growth, showed no significant NDVI–grain yield relationship, suggesting that NDVI values in this group were influenced by sterile tillers rather than contributing to yield. These findings provide valuable insights for crop breeders by offering a non-destructive approach to enhancing genetic gains through the improved selection of resilient wheat genotypes.

The Cycles agroecosystem model: Fundamentals, testing, and applications

Models of the soil–plant-atmosphere continuum are repositories of knowledge and gears in analytical and decision support tools applied to agroecosystems. In this paper, we present the Cycles agroecosystem model theory along with test cases and applications. Cycles combines innovations for simulating soil hydrology and biogeochemistry, including carbon and nitrogen saturation theory, with a modular software architecture. These elements enable simulating monoculture or polyculture crop sequences and associated management practices, containerizing for applications that require high-performance computing, and data assimilation at runtime. A comparison of simulated and measured daily evapotranspiration (ET) obtained with the eddy covariance method for maize (Zea mays L.) and shrub willow (Salix spp.) shows that Cycles represents well meteorological and vegetation controls of ET (root mean square error or RMSE = 0.75 mm d-1). Cycles accurately simulated differences of 150 mm in growing season ET between these two plant communities. Comparisons of modeled versus measured soil water content under soybean (Glycine max [L.] Merr.) in southeastern Pennsylvania for six soil layers at 0.1-m increments show accurate representation of water depletion and recharge (RMSE of 0.027–0.011 m3 m−3). Simulations of growth and nitrogen uptake of wheat (Triticum aestivum L.) in eastern Washington also highlight the model’s skill simulating processes that affect water and nutrient fluxes simultaneously. To highlight Cycles’ suitability for incorporation in high performance computing applications, we present a coupling of Cycles with an autonomous crop sequence builder (Cycles-A) in the Chesapeake Bay watershed. This system automatically identified areas for double cropping and selected the optimum combination of annual crops across the watershed. The Cycles model innovations and agroecosystem framing continue advancing the premise of making models not only dynamic knowledge repositories but useful tools for research and landscape management.

Elucidation of biochemical and physiological modulations in Triticum aestivum induced by green synthesized nitrogen-enriched zinc nano-complexes

This study investigates the efficacy of a green synthesized nitrogen-rich zinc complex (Zn-NC) using quinoline (C9H7N) as the nitrogen-rich substrate to enhance growth and biochemical properties in wheat (Triticum aestivum). The performance of Zn-NC was compared to standard zinc oxide nanoparticles (ZnO-NPs). Both Zn-NC and ZnO-NPs were characterized using scanning electron microscopy (SEM) and X-ray diffraction (XRD), and dynamic light scattering (DLS). Three concentrations (100, 200, and 500 ppm) of each compound, along with a control, were applied to local soil samples (n=3). The physiological (biomass, elongation) and biochemical effects (chlorophyll, carotenoids, flavonoids, and phenols) on wheat were investigated. Potential phytotoxic effects were evaluated to establish the biostimulants' safety thresholds. Plants treated with green Zn-NC showed an average increase in shoot length of 25 % compared to the control group. The chlorophyll content in plants treated with ZnO-NPs increased by 18 %, while those treated with green Zn-NC increased by 12 % compared to control. Application of ZnO-NPs resulted in a 30 % increase in total yield, whereas green Zn-NC treatment led to a 22 % yield increase. The root biomass of plants treated with ZnO-NPs increased by 28 %, and those treated with green Zn-NC saw a 20 % increase compared to controls. Based on the optimization of overall results, the ZnO NPs showed phytotoxic effects at concentrations above 200 ppm, while green Zn-NC exhibited no significant phytotoxicity even at concentrations up to 300 ppm. This study delineates the optimal concentrations of Zn-NC and ZnO-NPs that can enhance nutrient delivery and yield in cereal crops while mitigating phytotoxic risks. The findings provide valuable insights into applying nano-biostimulants in agroecosystems, highlighting their potential to improve productivity and sustainability in agriculture.

Comparative effects of micron-sized silicon sources and Si nanoparticles on growth, defense system and cadmium accumulation in wheat (Triticum aestivum L.) cultivated in Cd contaminated soil

Elevated levels of cadmium (Cd) in soils posesignificant challenges to global agricultural crop production. The potential of silicon (Si) nanoparticles (NPs) and biogenic Si sources of different sizes to mitigate Cd stress in wheat remains largely unexplored. A pot experiment was conducted to evaluate the effectiveness of various sizes of biogenic Si sources: rice husk biochar (RHB), sugarcane bagasse (SB), and rice straw (RS); categorized by particle size (<150 μm, 150–250 μm, and 250–1680 μm, denoted as S1, S2, and S3, respectively), and Si NPs on wheat growth and Cd accumulation. The application of RHB (<150 μm) significantly increased plant height (105 %), spike length (94 %), shoot dry weight (93 %), root dry weight (83 %), and grain weight (89 %) compared to the control. The RHBS1 treatment delivered the greatest reduction in the bioavailable fraction of soil Cd (-74 %), as well as Cd levels in roots (-85 %), shoots (-93 %), and grains (-97 %) compared to the polluted control. Additionally, RHB (<150 μm) showed the highest increase in antioxidant enzyme activities compared to the Cd-spiked treatment. Interestingly, the various biogenic Si sources have the potential to mitigate Cd stress in wheat plants. Further research is required to understand the underlying mechanisms and to investigate the effectiveness of different biogenic Si sources and particle sizes in alleviating Cd stress in crops.

Effect of seed priming and seed rate on the performance of wheat (Triticum aestivum)

A field experiment was carried out to determine the impact of different seed rates and priming strategies on germination percentage, growth attributes and yield of wheat. The experiment comprised of twelve treatments i.e. dry seed surface seeding; 12 hour soaked seed surface seeding; seed priming with 1% KNO3 and seed priming with 1% CaCl2 at 100, 125 and 150 kg/ha seed rate. The results indicated that highest final germination percentage (84 %) at 10 DAS was found with seed priming with 1% CaCl2 at 100 kg/ha seed rate. The tallest plant was observed with seed priming with 1% CaCl2 at 150 kg/ha seed rate. However, number of tiller/m2 and dry matter accumulation was recorded maximum with seed priming with 1% CaCl2 at 150 kg/ha seed rate, which was 7.27% and 20% higher compared to number of tillers/m2 and dry matter accumulation under dry seed surface seeding with 100 kg/ha seed rate respectively. Among different treatments, highest grain yield (4662 kg/ha) and straw yield (5694 kg/ha) were found with seed priming with 1% CaCl2 at 150 kg/ha seed rate. Similarly, maximum gross (₹114851/ha) and net (₹76884/ha) returns were obtained in seed priming with 1% CaCl2 at 150 kg/ha seed rate but highest B: C ratio (3.09) obtained with seed priming with 1% CaCl2 at 125 kg/ha seed rate. Hence, adopting seed priming strategies at different seed rates can improve growth and yield of wheat.

Yields, nutrient-use efficiencies and production economics of wheat (Triticum aestivum) as influenced by foliar nano urea

The nano fertilizers are becoming popular in crop production sector due to their higher nutrient use efficiency and also enhance the productivity and quality while requiring less input and being environmentally safe. In order to assess the effect of foliar application of nano urea on growth and yield performances of wheat (Triticum aestivum L.) and to evaluate nitrogen-use efficiency under integrated use of nano urea, this study was undertaken during the rabi seasons of 2021–22 and 2022–23 at the Uttar Banga Krishi Viswavidyalaya, Pundibari, West Bengal. The experiment was laid-out in a randomized block design (RBD) having 13 treatments consisting of foliar application of water, nano urea (once at tillering and twice at tillering and jointing) and 5% urea in combination with each of 50,75 and 100% of recommended nitrogen doses (RDN) along with a control (without N), and each treatment was replicated thrice. It was observed that foliar application of nano urea (0.4%) twice at tillering and jointing stages with 100% (RDN 5,759 kg/ha) was statistically at par with 100% RDN + 5% foliar spray of urea twice at tillering and jointing stage (5,985 kg/ha) in terms of grain yield, the latter being the highest yielder. Foliar application of either urea or nano urea by curtailing 25-50% RDN did not brought about superior growth and yield performances. Under each level of RDN (100%, 75% and 50%), foliar application of 5% urea was a bit superior to nano urea, though the difference was not statistically significant. However, nano urea reflected higher nitrogen-use efficiency under all levels RDN; partial factor productivity of nitrogen (PFPN) and agronomic nitrogen-use efficiency (ANUE) values be- ing the highest under 50% RDN along with two spraying of nano urea (56.76 and 24.50 kg grain/kg N). The treat- ment comprising 100% RDN+ 5% spray of urea at tillering and jointing stages resulted in the highest net return (69608 `/ha) and B: C ratio (2.58), closely followed by the treatment comprising nano urea application twice (gross return, net return and B: C ratio of 109,426 `/ha, 63,748 `/ha and 2.40, respectively) with 100% RDN, being statis- tically at par with each other. It can be concluded from this two-year trial that only foliar application of nano urea by curtailing soil nitrogen application to 25-50% resulted in reduced yield performances of wheat; nano urea had pro- motive effects on yields when it was applied along with 100% RDN.

Impact of fertilizer levels and treated sewage water on heavy metal uptake,growth, agrometeorological indices, and quality of wheat

A field experiment was conducted at Vegetable Research Farm, CCS Haryana Agricultural University, Hisar, Haryana, India to study the agro-physiological, agrometeorological, heavy metals, and quality parameters of wheat under different fertilizer levels and treated sewage water application on sandy loam soil. Among varieties, signifi- cantly higher yield attributes viz effective tillers and yield were observed with variety HD 3086 being at par with WH 1105 over HD 2967, WH 1124, and DBW 90. A higher value of agrometeorological indices viz. thermal (2.67 kg/ ha/0 C day), helio-thermal (0.77 kg/ha degree day hr), and photothermal (0.23 kg/ha degree day hr) use efficiencies were found with variety HD 3086 over other varieties. Among fertilizer levels, significantly higher yield attributes and grain yield were found with 125 % RDF over 75 and 100 % RDF. Significantly higher values of thermal, heliothermal, and photothermal use efficiencies were observed with the application of 125 % RDF over 75 and 100 % RDF Neither the varieties nor the fertility levels bring any significant variation in normalized difference vegeta- tion index and canopy temperature. However, varying levels of fertilizer did have a substantial impact on chloro- phyll content. Similarly, quality parameters viz. hectoliter weight and protein content were not affected significantly by varieties and fertilizer levels. However, sedimentation value was significantly affected by varieties and fertilizer levels. Heavy metal concentration was found in wheat grain, but these were within the permissible limit.

Development of new sustainable pyridinium ionic liquids: From reactivity studies to mechanism-based activity predictions.

This study addresses the development of sustainable pyridinium ionic liquids (ILs) because of their potential applications in agriculture and pharmaceuticals. Pyridinium-based ILs are known for their low melting points, high thermal stability, and moderate solvation properties. We synthesized three novel pyridinium-based ILs: 1-(2-(isopentyloxy)-2-oxoethyl)pyridin-1-ium chloride, 1-(2-(hexyloxy)-2-oxoethyl)pyridin-1-ium chloride, and 1-(2-(benzyloxy)-2-oxoethyl)pyridin-1-ium chloride. The biological activities of these compounds were evaluated through plant growth promotion, herbicidal, and insecticidal assays. Our results show that the benzyloxy derivative significantly enhances wheat and cucumber growth, whereas the isopentyloxy compound has potent herbicidal effects. Computational methods, including DFT calculations and molecular docking, were applied to understand the structure‒activity relationships (SARs) and mechanisms of action. The computational techniques involved dispersion-corrected density functional theory (DFT) with the B3LYP functional and the 6-311G** basis set. Grimme's D3 corrections were included to account for dispersion interactions. The calculations were performed via GAMESS-US software. Quantum descriptors of reactivity, such as ionization potential, electron affinity, chemical potential, and electrophilicity index, were derived from the HOMO and LUMO energies. Molecular docking studies were conducted via the CB-Dock server via AutoDock Vina software to predict binding affinities to cancer-related proteins. Petra/Osiris/Molinspiration (POM) analysis was used to predict the drug likeness and other pharmaceutical properties of the synthesized ILs.

Distinct accumulation patterns, translocation efficiencies, and impacts of nano-fertilizer and nano-pesticide in wheat through foliar versus soil application

The use of nano-chemicals in agriculture has been shown to enhance crop production through soil additions or foliar sprays. However, the accumulation pattern, translocation efficiency, mode of action of nanomaterials (NMs) via different application methods remain unclear. In this study, wheat was treated with CuO-NPs/CeO2-NPs (50 and 100 nm) for 21 days using soil and foliar application separately. Foliar spray resulted in higher accumulation and more efficient translocation of NMs compared to soil addition. Smaller NMs exhibited higher accumulation and transfer capabilities under the same application method. The accumulation of CuO-NPs was approximately 20 times greater than that of CeO2-NPs, particularly under the soil addition treatment. Scanning electron microscopy analysis demonstrated that NMs could directly enter wheat leaves via stomata during foliar application. Wheat growth was inhibited by roughly 15 % following CuO-NPs exposure, whereas no significant effects on growth were observed with CeO2-NPs. By integrating nontargeted metabolomics analysis with targeted physiological characteristics assessments, it was revealed that CuO-NPs mainly disturbed nitrogen metabolism pathways and induced oxidative damage. In contrast, CeO2-NPs enhanced carbohydrates related biological processes such as starch and sucrose metabolism, glycolysis, and TCA cycle, which are crucial for carbon metabolism. These findings suggest that the type of nanomaterial is a crucial factor to consider when evaluating their foliar or soil application in agriculture.