Tooth Growth Research Articles

HUBUNGAN AIR SUSU IBU EKSKLUSIF DENGAN JUMLAH GIGI SUSU BAYI USIA 6-12 BULAN DI WILAYAH KERJA PUSKESMAS BOTANIA KOTA BATAM

Background: The incidence of delayed growth and development of bones and teeth, as well as abnormalities in bone and teeth among the Indonesian population, is quite high. The growth of baby teeth provides an indication of infant nutrition, as sufficient nutrition influences the acceleration of baby teeth growth. Thus, essential nutrients for overall metabolism, foods with high-quality protein and calcium concentration, have a linear relationship with the growth and development of bones and teeth. Methods: : The study employed an analytical observational research design with a cross-sectional approach. The population consisted of 486 infants aged 6-12 months from the Botania Community Health Center. Sample selection utilized Simple Random Sampling with a minimum total sample of 78. Data were analyzed using the Chi-Square Test. Results: There was a significant relationship between Exclusive Breastfeeding and the Number of Baby Teeth Aged 6-12 Months at the Botania Community Health Center, with a obtained p-value of 0.024 < 0.05. Conclusion: Based on the research findings, it is concluded that there is a significant relationship between the Number of Baby Teeth Aged 6-12 Months in the working area of Botania Community Health Center, Batam City, in 2023, with a p-value of 0.024. Keywords: Exclusive Breastfeeding, Infants aged 6-12 months, Baby Teeth

Successful Anesthetic Management for Mandibular Neoplasm Resection in a Patient with Osteogenesis Imperfecta: A Case Report

Background: Osteogenesis imperfecta (OI) is a rare genetic disorder characterized by bone fragility and deformities. Patients with mandibular neoplasms and suspected OI require careful anesthetic management to avoid complications. This case report describes the successful anesthetic management of a patient with a mandibular neoplasm and suspected OI. Case presentation: A 33-year-old man presented with a large mandibular tumor causing airway obstruction and difficulty eating. He had physical features suggestive of OI, including short stature, bone deformities, and abnormal tooth growth. Preoperative evaluation revealed a difficult airway due to the tumor and potential cervical spine instability. Anesthesia was induced with propofol and atracurium after securing the airway via ultrasound-guided tracheostomy under local anesthesia. The tumor was resected successfully, and the patient recovered without complications. Conclusion: Anesthetic management in patients with suspected OI and mandibular neoplasms requires careful planning and execution. A multidisciplinary approach, including preoperative evaluation, airway management strategies, and close postoperative monitoring, is crucial for successful outcomes.

Dental trauma in primary dentition and possible outcomes on permanent successors

Objectives. The aim of this study is to determine if there is a correlation between a traumatic dental injury suffered by a deciduous tooth and the alterations that may appear in the structure of the permanent one. Materials and methods. For this narrative review, we collected articles written in English from PubMed, Scopus, and Embase. A total of 116 papers were assessed, with 20 included in the review. Results. The review found that there is a strong relationship between a traumatic incident suffered by the temporary tooth and the developmental disturbances that appear in the permanent successors. The majority of traumatic dental injuries take place around the age of 1-3 years, when the child is learning how to walk. The developmental disturbances that may appear are categorized as mild or severe, with the difference between them being the age of the child when the trauma occurred, the type of trauma, the degree of root resorption, and the force of the impact. Conclusion. The amount of injury to a primary tooth without address it when it happens has detrimental effects on the growth and emergence of permanent teeth, requiring more prolonged and costly treatment.

Comparison of the accuracy and bias of Demirjian’s method for East Asian children and development of Japanese population-specific age estimation formulae

Despite the increased global mobility owing to the development of the international community, there remains a need for population-specific methods to estimate children’s ages. Therefore, this study aimed to substantiate the necessity for a Japanese-specific age estimation method by contrasting the formerly reported age estimation accuracy and bias using Demirjian’s method for Chinese, Taiwanese, South Korean, and Japanese children. We analyzed 1,558 panoramic radiographs from Japanese children (777 boys and 781 girls), assessed the maturity of seven left permanent teeth using Demirjian’s criteria, and calculated the estimated age using Demirjian’s method. The accuracy of the estimated ages was compared with previous reports of children from other East Asian countries which used the same age estimation method. Chinese, Taiwanese, and South Korean boys and girls were all reported to be older than their chronological ages, with the Eastern and Northern Chinese regions being the only exceptions. The same was true for Japanese children: the estimated ages of both sexes tended to be higher than their chronological age. However, there were significant variations in the values, indicating the differences in tooth growth and development between East Asian countries and sexes. Therefore, new regression equations specific to the Japanese population were formulated, and their accuracy was evaluated as the final result of this study.

A Review on Dental Age Estimation in Children and Adolescents

Abstract Children are seen as beings who exist from birth through puberty, while teenagers are regarded as existing from puberty until around the age of 20 years. For a number of legal processes, including child labor, employment, the age of majority, rape, adoption, marriage eligibility, and situations where the birth certificate is unavailable, age estimation in children and adolescents is crucial. Despite the wide range of methods available, dental age estimation techniques that take into account tooth maturation are thought to be the most reliable predictors of chronological age in subadults. This is because genetic factors predominate and environmental factors, particularly between birth and age ten, tend to have little impact on tooth maturation. The eruption of teeth holds greater significance in the deciduous dentition, where genetic factors predominantly govern the process, compared to the permanent dentition. Conversely, tooth calcification serves as a viable indicator for estimating dental age in both primary and permanent dentitions. Current dental age estimation methods are based on age-related changes in teeth, such as tooth growth and development, changes that occur after teeth form, and biochemical changes. Therefore, in this review article, we will explore the several methodologies used for dental age assessment in children and adolescents.

Testing the Sample Preparation Method and Oxygen Isotopic Analysis of Tooth Enamel for the Reconstruction of the Birth Seasonality of Ovicaprines (the Case of Teeth from Istykskaya Cave, Eastern Pamir)

Cyclic variations of δ18O along the growth line of M2 and M3 molars provide information about the seasonality of enamel formation and thereby about reproductive seasonality in animals, taking into account the time of tooth eruption and full enamel maturation. Determination of birth seasonality of small ruminants is relevant to the reconstruction of pastoralist strategies. Two peaks of reproductive activity per year are one of the most reliable indicators of human control of the small ruminants. As part of this work, for the first time in Russia, a method of sample preparation and analysis of oxygen isotope ratios in the tooth enamel of small ruminants is proposed. Traditionally, breeding seasonality was evaluated by the isotopic analysis of carbonates, the content of which in dental enamel is only 4 %. According to a new approach, oxygen from a sample is converted to gaseous CO by the interaction of enamel with carbon in a helium flow under 1300 °С, enabling one to measure δ18O in all components of the dental enamel, including phosphates, which make up 90 % of enamel mass and are resistant to diagenesis. In this study, four sample preparation protocols depending on the degree of preservation of teeth and their age were tested: (A) cleaning and sampling of enamel, (B) treatment by H2O2, (C) treatment by NaOCl and CH3COOH, and (D) extraction of Ag3PO4. Results show that for assessing seasonality breeding, it suffices to evaluate the lowest and the highest δ18O along the tooth growth line. If the preservation of sample is good, minimal chemical treatment is enough to observe these extremes.

Vole genomics links determinate and indeterminate growth of teeth.

Continuously growing teeth are an important innovation in mammalian evolution, yet genetic regulation of continuous growth by stem cells remains incompletely understood. Dental stem cells responsible for tooth crown growth are lost at the onset of tooth root formation. Genetic signaling that initiates this loss is difficult to study with the ever-growing incisor and rooted molars of mice, the most common mammalian dental model species, because signals for root formation overlap with signals that pattern tooth size and shape (i.e., cusp patterns). Different species of voles (Cricetidae, Rodentia, Glires) have evolved rooted and unrooted molars that have similar size and shape, providing alternative models for studying roots. We assembled a de novo genome of Myodes glareolus, a vole with high-crowned, rooted molars, and performed genomic and transcriptomic analyses in a broad phylogenetic context of Glires (rodents and lagomorphs) to assess differential selection and evolution in tooth forming genes. We identified 15 dental genes with changing synteny relationships and six dental genes undergoing positive selection across Glires, two of which were undergoing positive selection in species with unrooted molars, Dspp and Aqp1. Decreased expression of both genes in prairie voles with unrooted molars compared to bank voles supports the presence of positive selection and may underlie differences in root formation. Bulk transcriptomics analyses of embryonic molar development in bank voles also demonstrated conserved patterns of dental gene expression compared to mice, with species-specific variation likely related to developmental timing and morphological differences between mouse and vole molars. Our results support ongoing evolution of dental genes across Glires, revealing the complex evolutionary background of convergent evolution for ever-growing molars.

Estimation of chronological age of Risso’s dolphin (Grampus griseus) based on DNA methylation frequency

Age of cetaceans is an important data set for their conservation and management. Although the chronological age of a toothed whale has traditionally estimated from the number of annual tooth growth layer groups (GLGs), this method has potential difficulties such as errors due to the subjectivity of the age-readers and time-consuming sample processing, in addition to lethal tooth sampling in many cases. To overcome these difficulties through a non-lethal approach, considerable amount of efforts has been made in recent years to construct chronological age estimation models based on the correlation between chronological and biological age expressed as the frequency of DNA methylation at CpG (cytosine-phosphate-guanine) sites. The present study, by using next-generation sequencing on DNA extracted from the skin tissues of 30 individuals (15 males and 15 females), performed methylation analysis on multiple CpG sites in the three genes previously reported as age-linked in cetaceans (GRIA2, TET2, and CDKN2A) to construct an age estimation model for Risso’s dolphins. Key aspects of this study are: (1) the chronological ages of Risso’s dolphins of wild origins used in this study had been determined by the GLGs of teeth prior to this study, and (2) as Risso’s dolphins are said to have a lifespan of 30 years, samples were collected from individuals aged 1–29 years, covering from young to old ages. Among the 11 CpG sites in GRIA2, 6 CpG sites in TET2, and 7 CpG sites in CDKN2A analyzed, the CpG sites in GRIA2 showed a statistically significant correlation with age, whereas those in TET2 and CDKN2A did not show such a strong correlation. Using multiple regression analysis on the CpG sites in the three genes, the best fitted model was expressed as follows: y=-0.7548 + 5.6164x(GRIA2_CpG3)+2.6187x(TET2_CpG6)-1.5907x(CDKN2A_CpG3) (R²=0.7062, residual mean=2.98, standard deviation=2.3). With the mean error of 2.98 years between the estimated and known chronological ages, the accuracy of this model was found to be comparable to or higher than that of previously reported age estimation models for cetaceans. This study demonstrated for the future that the age of wild Risso’s dolphins can be estimated by using their skin samples collected with minimal invasiveness.

Teeth pathology of horse from the khirgisuur satellite burials in Orkhon valley

The purpose of this research is to clarify the dental diseases of horses in the Khirgisuur satellite burials of the Late Bronze Age of Mongolia, and to discuss the age and sex of the selected horses. The study included 96 horse bones from #OOR-396th of Khirgisuur satellite burials in the Saikhan Bulgiin denj. The survey was conducted according to standard zooarchaeological methods. According to the results of the research, 35.4% of all horses had tooth decay, jaw inflammation or tumour, and tooth growth defects. It was also observed that young horses were predominantly used for sacrifice. In conclusion, in some cases, choices have been made to support the healthy growth of horses.

On the issue of the manufacture of ceramic structures in orthopedic dentistry

The article presents a review of ceramic materials used in modern orthopedic dentistry. Currently, the main types of orthopedic structures containing dental porcelain are metal-ceramic, metal-free ceramic crowns, as well as prostheses made using pressed ceramic technology, in particular veneers. All types of prostheses produced using dental porcelain have a common manufacturing technology, which consists in the sequential application of ceramic layers with their subsequent firing. Moreover, for each group of teeth, there are additional masses for firing, for example, masses of the cutting edge, masses simulating various effects (for example, blue, yellow and other shades, imitation of tooth growth lines). These masses are created in order to give future crowns the most natural and aesthetic appearance. All structural components (layers) of ceramics used in the manufacture of ceramic prostheses have a similar chemical composition. The raw material is feldspar glass with inclusions of crystalline quartz. Depending on the conditions of the reactions, the composition of mixtures can be varied and compounds with desired properties can be obtained using the phase analysis method, which will expand the range of domestic ceramic materials produced, which are currently quite scarce. This is especially relevant in modern conditions of import substitution.

The crucial role of centrioles in tooth growth and development

The crucial role of centrioles in tooth growth and development

Computed Tomographic Findings of Dental Disease and Secondary Diseases of the Head Area in Client-Owned Domestic Rabbits (Oryctolagus cuniculus): 90 Cases.

Domestic rabbits have teeth that grow throughout the animal's life and are prone to disease. Clinical signs of dental disease in domestic rabbits are non-specific, and, therefore, a definitive diagnosis usually requires additional methods. This study was carried out on a group of 105 domestic rabbits aged 3 to 9 years. In total, 90 domestic rabbits with dental disease visible on CT images and other secondary diseases of the head area qualified for this study. Malocclusion was found in 57 (63.3%). Retrograde elongation of the tooth apices in the mandible was present in 39 (43.3%), and it was present in the maxilla in 48 (53%). Clinical tooth crowns were overgrown in 39 (43%). Dental abscesses were present in 54 (63%). Secondary to the presence of a dental abscess, osteomyelitis was found in 43 (79% of the animals with a dental abscess). Dental inflammatory resorption was found in 36 (40%). Secondary to dental disease, nasal cavity inflammation was found in 18 (20%). Otitis media was present in six (6.7%). The most common dental disease found in this study's animals was malocclusion secondary to abnormal clinical crown abrasion and abnormal tooth growth. In domestic rabbits, osteomyelitis is a common complication of dental abscesses. Computed tomography is an invaluable diagnostic method in the diagnosis of dental disease and secondary diseases of the head area, such as inflammation of the nasal cavities or otitis media, in pet rabbits.

Klasifikasi kualitas mutu susu pasteurisasi menggunakan metode klasifikasi k-Nearest Neighbor

Milk is a product that is often consumed because it has many good benefits for the body. One of the benefits of milk is that it contains calcium, which is useful for the growth of bones and teeth. However, many consumers still choose dairy products based only on their appearance, even though milk quality is not based on its appearance but is found during milk processing. Pasteurization is the process of heating whole milk at a specific temperature and for a certain period to increase the milk's shelf life and maintain the milk's quality. Two types of pasteurization processes are Long Temperature Long Time and High Temperature Short Time. k-Nearest Neighbor or k-NN is a data mining method used to classify objects from the data, using distance calculations or Euclidean Distance to look for similarities between neighbors. So, based on this problem, milk quality classification is carried out. The research was carried out by classifying milk quality using the K-NN method. The public data used to classify is 1,059 pasteurized milk data, with seven regular or ordinary attributes and one special or class attribute. The K values ​​that will be used in this research are k=3, k=5, and k=7. From the analysis of calculation results using the k-NN method, the accuracy values ​​obtained are k=3 is 97%, k=5 is 89%, and k=7 is 87%.

Assessment of dental morbidity in school-going children (6–10-year-old): A cross-sectional study

Background Restricted activity days are prevalent in children with oral health problems. Oral health issues cause over 50 billion hours of education to be lost each year, which has an impact on children’s school performance and the potential for success. Mostly it is concluded that dental caries is the most common disease among individuals. In addition, it may create chronic systemic conditions in healthy individuals. In children, it may induce deformities in the growth of the unerupted teeth. This study will help identify the factors and causes of risk factors for morbidities associated with dental problems. It also includes the educational status and socioeconomic condition of their parents, which is conducted in the Wardha district of Maharashtra in the field practice of villages among children. Aim To Assess dental morbidity among schoolchildren in rural areas and their relationship with their socioeconomic status in rural areas of Wardha District. Methodology A questionnaire-based face-to-face interview was conducted in this community-based cross-sectional study to assess dental morbidity in school-going children. The study participants will be children aged 6–10 years in the rural areas of the Wardha district. The variables of this study were the sociodemographic profile, habits, and morbidities of the children.

Increasing Children's Capacity At Al-Azhar Syifa Budi Kindergarten To Maintain Healthy Teeth

Children's teeth are a determinant of the growth and development of the oral cavity because the child's milk teeth will determine the child's permanent teeth. Therefore It is important to monitor and monitor the growth of teeth in children here. The aim of promoting health on the correct way to brush your teeth to students is to increase students' knowledge about the steps for brushing teeth, how to brush teeth correctly, and demonstrate the correct way to brush your teeth with the help of a dental phantom, so students can get used to it. to clean and brush teeth. Lifetime live in everyday life. This exercise uses the lecture and demonstration method brush teeth, question and answer and discussion. Before counseling is carried out, a post test is given to students as material evaluate activities to find out students' knowledge about brushing teeth correctly before extension activities and after extension activities. After the lecture and demonstration/demonstration. A question and answer session was held after the counseling material was completed delivered to participants and delivered. From the results of the tooth brushing education, it can be concluded as follows: Al-Azhar Kindergarten student Syifa Budi can understand the stages of brushing teeth. Al-Azhar Syifa Budi Kindergarten students can practice/demonstrate the method brush your teeth properly and correctly. Al-Azhar Kindergarten student Syifa Budi understands how to maintain healthy teeth

Life-history of Palaeoloxodon antiquus reveals Middle Pleistocene glacial refugium in the Megalopolis basin, Greece

The Balkans are considered a major glacial refugium where flora and fauna survived glacial periods and repopulated the rest of Europe during interglacials. While it is also thought to have harboured Pleistocene human populations, evidence linking human activity, paleoenvironmental indicators and a secure temporal placement to glacial periods is scant. Here, we present the first intra-tooth multi-isotope analysis for the European straight-tusked elephant Palaeoloxodon antiquus, on an adult male individual excavated in association with lithic artefacts at the MIS 12 site Marathousa 1 (Megalopolis basin, Greece). The studied find also exhibits anthropogenic modifications, providing direct evidence of hominin presence. We employed strontium, carbon and oxygen isotope analysis on enamel bioapatite to investigate its foraging and mobility behaviour, using a sequential sampling strategy along the tooth growth axis of the third upper molar, to assess ecological changes during the last decade of life. We found a geographically restricted range, in a C3-dominated open woodland environment, and relatively stable conditions over the examined timeframe. Our results show that, despite the severity of the MIS 12 glacial, the Megalopolis basin sustained a mesic habitat, sufficient plant cover and limited seasonal fluctuations in resource availability, pointing to its role as a glacial refugium for both fauna and hominins.

AGE ESTIMATION OF PERMANENT TEETH AND ITS CORRELATION AMONG LOCAL POPULATION

Aims and Objectives: The aim of this study was to establish the relationship between the ages of the eruption of permanent teeth in correlation with the local population and related factors. Methods: The present prospective observation study was conducted on one thousand students in schools and colleges aged 6–25 years by the Department of Forensic Medicine and Toxicology, Government Medical College, Amritsar. The data collected from this study were organized by presenting it in appropriate tables and graphs, which are statistically analyzed for percent ages and inferences. Results: The first permanent tooth to erupt is the first permanent Molar, which erupts at 75.9–77.9 months; permanent central incisor is the second permanent tooth to erupt following the first Molar. The age of eruption is 80.8–84.0 months. Permanent Lateral Incisor erupts at the age of 105.2– 107.4 months, and permanent Premolar one teeth erupt at the age of 109.5–112.6 months, permanent premolar two teeth erupt at the age of 131.0– 133.8 months, permanent Canine teeth erupt at the age of 142.6–144.8 months. Conclusion: It is thus concluded, based on the present study that the growth and eruption of teeth depend on various factors, including genetic as well as ecological aspects. The most accurate method, which depends on the conditions as well circumstances of each case, namely, the elements can be age, people participating in the study, supporting networks, availability of tools of age estimation, culture, religion, and ethnicity of the individual.

Management of Post-traumatic Dental Immature Teeth at the Growing Phase

Background: Anterior dental trauma is common in children. Traumatic dental injury in children's teeth can interfere with the function of speech, mastication, aesthetics, eruption of permanent teeth, and the growth and development of teeth and jaws. An unclosed and wide-open apex (immature tooth) is a complex case for a pediatric dentist due to multiple stages of treatment and different options compared to adults. Objective: To explain the management of post-traumatic dental immature teeth. Case: An eight-year-old male child came to the clinic one month after getting a dental injury. This case is classified into Class IV Ellis and Davey's traumatic dental injury classification with immature teeth. Case Management Apexification uses Ca(OH)2 paste followed by an acrylic provisoris restoration while the jaw grows. Conclusion: Apexification using Ca(OH)2 paste followed by acrylic provisions crown restoration is a proper treatment in this case.

Exploring the effects of methodological choices on the estimation and biological interpretation of life history parameters for harbour porpoises in Norway and beyond.

This study investigates effects of subtle methodological choices on the estimation and biological interpretation of age, growth and reproductive parameters for harbour porpoises. The core analyses are based on a focal Norwegian data set built on samples from 134 harbour porpoises caught incidentally in gillnet fisheries along the Norwegian coast during autumn 2016 and spring 2017. Two contrasting practices for interpretation of seasonal and ontogenetic characteristics of tooth growth layer formation resulted in significant age differences among spring samples of young porpoises and for older animals across seasons. In turn, these differences affected estimates of age at maturity and asymptotic lengths, respectively. We also found significant differences in male age at maturity between two well-documented maturity criteria and between mathematical estimators of age at maturity for both sexes. Two different criteria for corpus albicans classification furthermore resulted in different patterns of ovarian corpora accumulation, which may affect some estimates of fecundity rates and contaminant loads. Both corpora accumulation patterns were also found in reanalysed data from German and Greenlandic porpoises. Based on tabulated overviews of methodological choices made in previous harbour porpoise studies, we argue that several of the issues mentioned above have wider relevance and may affect the validity of meta-analyses as a tool for estimating harbour porpoise sensitivity to extrinsic pressures. Differences in cause of death (COD) composition between data sets can have a similar effect. We demonstrate this in a meta-analysis of published harbour porpoise pregnancy rates, showing significantly higher values for trauma-killed samples compared to samples comprising mixed COD categories. COD also affected the estimated impacts of three previously analysed extrinsic predictors as well as an added predictor for vessel noise levels. We discuss the potential contributions of methodological, biological and anthropogenic factors in shaping observed regional differences in estimates of harbour porpoise life history parameters.

Revolution of Bone and Teeth Health: Study of Aloe Barbadensis Instant Powder Formulation

Aloe barbadensis is a plant with many applications such as anti-inflammatory, anti-fungal, anti-bacterial, and aiding in cell regeneration. Understanding aloe barbadensis' phytochemical profile and pharmacological action is essential since it is believed to have an impact on the formation of teeth and bones. The stability and bioavailability of Aloe barbadensis can be improved by formulating it as an instant powder. The research's objectives are to ascertain the Aloe barbadensis instant powder's qualitative and quantitative phytochemical profile, dosage formulations, and activity testing on hemoglobin, cholesterol, and red blood cell parameters. Samples of aloe barbadensis were washed, and they were then dried for 72 hours at 50°C. Following a maceration process using a 70% ethanol solvent, the extract was dried. Phytochemical screening, TLC profile, and extract description were employed to test the extract qualitatively. The quantities of total flavonoids, total anthraquinones, and total phenolics were determined to quantitatively test the extract. The formulation of the instant powder was then completed and evaluated on female mice using metrics associated with red blood cells, hemoglobin, and cholesterol levels. Furthermore, observations were made on the mice's liver organs. The study's findings revealed a qualitative profile of Aloe barbadensis extract, which included a tasteless, unique odor, milky white hue, and liquid shape. Aloe barbadensis has been demonstrated to contain flavonoids, phenolics, tannins, saponins, and anthraquinones, according to the results of phytochemical screening. Three spots, identified as Rf 3.2 and Rf 8.5 in the Rf 2.3 area, are visible in the chromatographic pattern. Total anthraquinones were discovered to be 4.59%, total flavonoids to be 0.24%, and total phenolic content to be 1.42%. The third formula of instant Aloe barbadensis pollen has been demonstrated through preclinical examinations to have the capacity to reduce cholesterol, boost hemoglobin, and enhance red blood cell count—all of which are associated with the growth of teeth and bones. An SPSS statistical study demonstrating statistically significant differences with other groups supports this. Mice liver histopathological examinations revealed no liver damage in any of the test groups.