Agarwood is an economically important native tree species of sub-tropical-tropical rainforests of northeast India. The species is included in the Convention on International Trade in Endangered Species of Wild Fauna and Flora. This study was carried out in the Department of Forestry during 2015–2018. The aim of this investigation was to measure the variability of seed traits. Field survey was carried out across the population of Aquilaria malaccensis throughout Mizoram, Nagaland, Assam, West Bengal and Tripura to select well represented nineteen seed sources viz. Basistha, Ganakpokhari, Hathipara, Hmarveng, Hybergyon, Islam nagar, Kumar ghat, Lanka, Lonigodam, Nagariborline, Naharani, Namti, Nazari, Nelbagan, Newdiakkawan, Rajabhatkhawa, Sephizala, Sukhana and Thikau. Nursery and field level study was carried out each respective seed source were raised in Randomized Block Design to assess growth performance w.r.t Plant height (cm), Collar Diameter (mm), Root length (cm), Fresh weight leaf (g), Fresh Weight of shoot (g), Fresh weight of root (g), Dry weight of leaf (g), Dry weight of shoot (g), Dry weight of root (g), Sturdiness Quotient (SQ), Dickson Quality Index (DQI), Volume index (cm.mm2), Total biomass (g), were recorded range respectively 40.03–47.23 cm, 4.856.72mm, 19.57–23.91cm, 3.43–5.68g,4.05–5.81g,2.01–3.18g, 0.96–1.89g, 0.83–1.70g, 0.76–0.96g, 7.55–8.58(SQ), 1.342.2 (DQI), 972.89–2132.83 cm.mm2 2.58–4.25g, were recorded Significant variations (p < 0.05).