Growth Habitat Research Articles

Nutrient requirements shape the preferential habitat of Allorhizobium vitis VAR03-1, a commensal bacterium, in the rhizosphere of Arabidopsis thaliana.

A diverse range of commensal bacteria inhabit the rhizosphere, influencing host plant growth and responses to biotic and abiotic stresses. While root-released nutrients can define soil microbial habitats, the bacterial factors involved in plant-microbe interactions are not well characterized. In this study, we investigated the colonization patterns of two plant disease biocontrol agents, Allorhizobium vitis VAR03-1 and Pseudomonas protegens Cab57, in the rhizosphere of Arabidopsis thaliana using Murashige and Skoog (MS) agar medium. VAR03-1 formed colonies even at a distance from the roots, preferentially in the upper part, while Cab57 colonized only the root surface. The addition of sucrose to the agar medium resulted in excessive proliferation of VAR03-1, similar to its pattern without sucrose, whereas Cab57 formed colonies only near the root surface. Overgrowth of both bacterial strains upon nutrient supplementation inhibited host growth, independent of plant immune responses. This inhibition was reduced in the VAR03-1 ΔrecA mutant, which exhibited increased biofilm formation, suggesting that some activities associated with the free-living lifestyle rather than the sessile lifestyle may be detrimental to host growth. VAR03-1 grew in liquid MS medium with sucrose alone, while Cab57 required both sucrose and organic acids. Supplementation of sugars and organic acids allowed both bacterial strains to grow near and away from Arabidopsis roots in MS agar. These results suggest that nutrient requirements for bacterial growth may determine their growth habitats in the rhizosphere, with nutrients released in root exudates potentially acting as a limiting factor in harnessing microbiota.

Material synthesis, crystal structure, Hirshfeld surface and physicochemical characteristics Novel Stilbazolium crystal of 4-(2-(3-ethoxy-4-hydroxy-phenyl)-vinyl)-1-methyl pyridinium iodide (MEMPI) single crystal for NLO activity

In this study, a novel cation donor group 3-ethoxy-4-hydroxy benzaldehyde has been incorporated in the Stilbazolium cation with iodide anion to generate a new NLO activity of a single crystal of 4-(2-(3-ethoxy-4-hydroxy-phenyl)-vinyl)-1-methyl pyridinium iodide (MEMPI). The MEMPI crystal was effectively formed via the solvent evaporation technique. The structure and hydrogen bond interactions found in the molecule were determined by employing single-crystal X-ray diffraction. The compound crystallizes in the space group P21/c and monoclinic crystal pattern. The crystal structures are stabilized via intramolecular, intermolecular hydrogen bond interactions, C‒H···π, cation‒π, anion‒π, π···π stacking and lone pair-π interaction. In addition, a variety of O‒H···O, O‒H···I and C‒H···O interactions are also responsible for crystal packing. The 3D Hirshfeld surface and 2D fingerprint plots were used to examine the nature of intermolecular interaction. The 2D fingerprint maps indicate that the interactions between H···H contribute to 48.9 % of the total crystal stability. The newly synthesized material was characterized by 1H NMR and 13C NMR. In addition, the vibrational study of the synthesized molecule was performed using FTIR analysis. The grown crystal in linear optical characteristics were examined via a UV–Visible-NIR study. The photoluminescence (PL) activity of a MEMPI was studied with respect to the visible area and green colour emission was observed at 536 nm. The estimated CIE coordinates on the RGB colour gamut show the MEMPI crystal's ability to emit green light. The chemical etching approach was used to analyse the etch pit density and the growth habitat of the MEMPI crystal. The closed and open aperture Z-scan tests were carried out to determine the nature of third-order NLO susceptibility of the MEMPI crystal.

Living with bats: polyphasic approach reveals a new Fusarium species in Yunnan Province, China

ABSTRACT Bats are one of the most diverse mammal groups, with over 1400 species distributed across the planet, and they provide key ecological and economic services. In addition, bats provide an ideal habitat for fungal growth and harbour a high diversity of fungal species. Previous studies have described 423 fungal species associated with bats and bat carcasses. This paper describes a new fungal species, Fusarium rhinolophi, belonging to Nectriaceae, collected from bats in Mengla County, Xishuangbanna Dai Autonomous Prefecture, Yunnan Province, China. Phylogenetic analyses of the combined ITS, CAM, TEF-1α, TUB, RPB1, and RPB2 dataset confirmed that our fungus forms an independent lineage within the F. nisikadoi species complex. Thus, we describe our fungus as a new species of Fusarium based on morphology and molecular phylogeny. A description, phylogenetic tree showing species placement, and micrographs are provided.

In vitro plant regeneration and bioactive metabolite production of endangered medicinal plant Fritillaria cirrhosa

The bulb of Fritillaria cirrhosa D. Don is widely used for the anti-asthmatic, anti-tussive, and anti-cancer agents, etc., while the yield is limited by an endangered status, a long juvenile phase, and restricted growth habitat. Ancillary approaches to improve the bulb yield by micropropagation and bioactive metabolites production by bioreactor have not been established. Here is reported the plant regeneration, suspension cell culture, and bioactive metabolite production at different treatments. The embryogenic calli were successfully induced via the histomorphological identification. The highest proliferation times (4.11-fold) were observed with a select combination of hormones [NAA (0.2 mg/L) + 6-BA (1.0 mg/L) + GA3 (1.0 mg/L)] and culture conditions (red light and 20 °C), the highest content of imperialine (0.13 mg/g) was observed under blue light, total phenolic (0.52 mg/g) under red light, polysaccharides (36.57 mg/g) and total flavonoids (2.67 mg/g) as well as antioxidant capacity under white light. The plantlets were regenerated within 125 d from the induced embryogenic calli to acclimation and transplantation of seedlings. For the suspension cell culture, a 6.30-, 1.78-, 1.37-, and 1.51-fold increase of proliferation times, imperialine, polysaccharides, and total phenolic contents was observed at 40 d, respectively. Based on the above observations, an effective and complete in vitro approach has been proposed to regenerate plants and produce bioactive metabolites in F. cirrhosa.

Facilitation drives tree seedling survival at alpine treelines

Abstract Alpine treelines are considered ecological monitors recording the impacts of climate change on trees and forests. To date, most treeline research has focused on how climate change drives treeline dynamics. However, little is known about how biotic interactions mediate treeline shifts, particularly in the case of tree recruitment, a bottleneck of treeline dynamics. We hypothesized that inter- and intraspecific facilitation determined the establishment and survival of tree seedlings at alpine treelines. To test this hypothesis, 630 Abies georgei var. smithii seedlings with different ages (4–6, 7–9 and 10–15 years old) were transplanted into three growth habitats (canopy-in, canopy-out and meadow) across the alpine treeline ecotone (4300–4500 m) in the Sygera Mountains, on the southeastern Qinghai-Tibetan Plateau. Microclimate, height growth, mortality rates and leaf functional traits of transplanted seedlings were measured over 3 years. We found that the variations in leaf functional traits were driven by microclimate. After the transplantation, the leaf concentrations of soluble sugars and starch and C:P ratio increased, whereas leaf size decreased. The resource use of seedlings gradually shifted to a more conservative strategy as indicated by changes in non-structural carbohydrates and nutrient concentrations. Radiation, temperature and moisture conditions, mediated by plant interactions, influenced seedling mortality and annual growth by affecting leaf morphological traits. Our findings illustrate how facilitation plays a crucial role in altering solar radiation and leaf trait functioning, determining seedling survival and growth at alpine treelines. We provide new insights into the underlying mechanisms for tree establishment and alpine treeline shifts in response to climate change.

Ribotyping Analysis of Microbes Producing Hydrolase from Batik Liquid Waste Samples

Batik liquid waste that is disposed of into the environment still contains high levels of organic compounds as residue from the main ingredients in batik production, like dyes, wax, starch, soda ash, and others. With its high organic material content, batik liquid waste is a potential habitat for microbial growth. This research aims to isolate and genetically analyze microbes in batik liquid waste, namely by ribotyping analysis of microbes that produce hydrolase enzymes. From the results of microbial screening on selective lipase media, eight types of bacteria were obtained which have the potential to produce hydrolase enzymes. Eight types of hydrolase-producing bacteria have been genetically identified using the 16S rRNA gene. Alignment analysis using blastN from NCBI of the 16S rRNA gene nucleotide sequence of the sample suggested that the hydrolase-producing microbial isolate of the sample had the closest homology to the Serratia, Burkholderia, Stutzerimonas, and Pseudomonas groups. Four of the eight samples, RR1R, RA1R, RA2R, and ZB16R, have the highest homology <97%. This suggests that the four samples are novel microbial variants, which are different from the microbial data in Genbank.

Environmental characterization and cartographic modeling of wild plant habitats at the northern coastal zone of Egypt

Assessing natural vegetation through conventional methods faces considerable constraints, such as limited geographical scope, reduced precision, a lack of historical data, high expenses and time demands. The study aims to use environmental and spectral data to identify and map natural vegetation and plant species along the Mediterranean coast of Egypt. This involves employing spatial analyses and cartographic modeling techniques, marking an initial effort in this endeavor. To fulfill this aim, a total number of 70 wild plant habitats were surveyed and sampled for further laboratory identification of plant species. Multispectral and thermal bands of Landsat imagery were processed to generate land cover map, as well as calculate Normalized Difference Vegetation Index (NDVI) and Land Surface Temperature (LST) to be integrated with the naturally grown plants in a cartographic model to predict the wild plant habitats. Coastal wild plant habitats were sparsely located and associated with a vegetation density ranging from 0.096 to 0.280 with an average of 0.167. Besides, the LST of these habitats fluctuated from 30.559 to 38.652 with an average of 34.361°C. Although the wild plant habitats at the Northern coastal region of Egypt are similar in environmental and climatic conditions, there are variability in NDVI and LST of each single habitat (pure or mixed). On the other hand, the lowest LST were associated with Erodium laciniatum/Rumex pictus and Erodium laciniatum habitats reporting 30.559 and 30.741°C, respectively. However, Lotus halophilus mixed with Ifloga spicata habitats reported the highest LST (38.652°C). The high vegetation density is mainly characterized by low LST which indicates the mixed wild plant habitats. The developed cartographic model showed a narrow strip along the Mediterranean region as highly suitable habitats for wild plant growth. The model can be used to map pure and mixed habitats of various wild plants with an accuracy exceeding 90%. The model was applied for mapping Arthrocnemum macrostachyum showing that the middle coastal region is most suitable for its growth. It’s recommended to integrate remotely sensed data with spatial analyses for the environmental analyses of natural plants. Present findings support researchers and scientists interested in environmental, botanic, and medical studies.

Exploring the eighteenth-century concept of soil as reflected in Sámuel Domby's De vino Tokaiensi

AbstractThe treatise De vino Tokaiensi (On Tokaj Wine) written by Sámuel Domby of Gálfalva (1729–1807), is a valuable source on Hungarian history of culture and science which has become widely accessible thanks to its facsimile edition. This medical doctoral dissertation published in 1758 in Utrecht presents a study of the medicinal effects of Tokaj wine, mirroring the norms of philosophical-scientific literature in eighteenth century Hungary. It is unequivocally an exceptional document of the intellectual heritage of the educated classes in the early modern age regarding growth habitat, viticulture and winemaking, with specific reference to Tokaj-Hegyalja, a wine region and cultural landscape of historic importance in Northeast Hungary. The present paper aims at identifying the perceptions detailed in the candidate's argument in pedological terms.

422 Mineral Content of Invaded Grassland Forages Is Affected By Precipitation and Management

Abstract Mineral content of grazed forage influences overall health, growth and reproduction of cattle and promotes maximum cattle productivity. However, grassland mineral content may not always meet cattle requirements throughout the grazing season and can be affected by precipitation of the season. Most of the Northern Great Plains grassland is invaded by exotic perennial grass species that have a uniform growth habit and response to precipitation. Growth habitat can be controlled by grazing or mowing, which may alter mineral content. No studies have examined the combined effects of mowing and drought on forage mineral concentration of invaded grassland. Therefore, the objective of this study was to determine mineral concentration of forage in rangeland pastures in seasons of low compared with normal precipitation and mowing status and model recommendations for mitigating low mineral content. This study was conducted at the Northern Great Plains Research Laboratory in Mandan, North Dakota USA. Pastures were sampled across several years and pasture locations, with enclosed plots mowed or not mowed in April, to simulate early season mobgrazing or clipping. Quarter- meter quadrats were taken every two weeks of the grazing seasons from plots in each pasture, one year dry and the other normal. Samples were analyzed for macrominerals and trace minerals via ICP-aa spectroscopy. Forage mineral content was analyzed as a completely randomized design using the MIXED procedure of SAS (SAS 9.4, Cary, NC). Total ash content was different by year (P = 0.0053) but not affected by mowing (P = 0.21). Potassium and phosphorus were not different due to mowing, nor effected by precipitation. Calcium was affected by mowing (P = 0.0065) and tended be affected by precipitation (P = 0.11). Zinc, copper, molybdenum, manganese and iron were all found to be significantly affected by precipitation (P < 0.001). When applied to a beef nutrient requirement model, Ca and P were generally adequate to meet recommended amounts for lactating cows based on a May-calving 1,200-pound cow most months of the season but did not meet needs of a 1400 lb. cow and calf. Forage phosphorus content was significantly less in June of each year, but adequate the rest of the season, suggesting targeting phosphorus supplementation for only the least months would save on mineral costs. Understanding forage mineral fluctuations across the grazing season may provide producers with tools to make the best supplementation strategies and use the most appropriate mineral formulations to maximize performance and reduce input cost.

Thermophilic Fungi as the Microbial Agents of Choice for the Industrial Co-Fermentation of Wood Wastes and Nitrogen-Rich Organic Wastes to Bio-Methane.

The novel industrial approach of co-fermenting wood wastes with agricultural wastes that are rich in nitrogen such as animal manures to produce bio-methane (renewable natural gas) fuel via thermophilic anaerobic digestion mimics an analogous process occurring in lower termites, but it relies instead on thermophilic fungi along with other thermophilic microorganisms comprising suitable bacteria and archaea. Wood microbial hydrolysis under thermophilic temperatures (range of 55 °C to 70 °C) and aerobic or micro-aerobic conditions constitutes the first step of the two-step (hydrolysis and fermentation) dry thermophilic anaerobic digestion industrial process, designated as "W2M3+2", that relies on thermophilic fungi species, most of which grow naturally in wood piles. Eleven thermophilic fungi have been identified as likely agents of the industrial process, and their known growth habitats and conditions have been reviewed. Future research is proposed such that the optimal growth temperature of these thermophilic fungi could be increased to the higher thermophilic range approaching 70 °C, and a tolerance to partial anaerobic conditions can be obtained by modifying the fungal microbiome via a symbiotic existence with bacteria and/or viruses.

High-affinity potassium transporter from a mangrove tree Avicennia officinalis increases salinity tolerance of Arabidopsis thaliana

Salinity reduces the growth and productivity of crop plants worldwide. Mangroves have evolved efficient ion homeostasis mechanisms to survive under their natural saline growth habitat. Information obtained from them may be utilized for increasing the salt tolerance of crop plants. We identified and characterized a high-affinity potassium transporter gene (AoHKT1) from Avicennia officinalis. The expression of AoHKT1 was induced by NaCl mainly in the leaves. Functional study by heterologous expression of AoHKT1 in Arabidopsis T-DNA insertional mutants athkt1–1 and athkt1–4 revealed that it could enhance the salt tolerance of the mutant plants. This was accompanied by an increase in K+ accumulation in the leaves. AoHKT1 was localized to the plasma membrane in Arabidopsis, and when expressed in yeast, it could complement the functions of both Na+ and K+ transporters. An attempt was made to identify the upstream regulator of AtHKT1, a close homolog of AoHKT1. Using chromatin immunoprecipitation, luciferase assay and yeast one-hybrid assays, WRKY9 was identified as the main transcription factor in the process. Furthermore, this was corroborated by the observation that AtHKT1 levels were significantly reduced in the atwrky9 seedlings. These findings revealed a part of the molecular regulatory mechanism of HKT1 induction in response to salt treatment in Arabidopsis. Our study suggests that AoHKT1 is a potential candidate for generating crop plants with increased salt tolerance.

Otolith microchemistry highlights the importance of extensive connectivity for conservation of an iconic migratory fish in a large tropical river basin

Abstract Migratory fishes exert important influences on tropical river food webs, but these species are often most vulnerable to environmental change. Movement patterns of fishes in tropical rivers are also generally poorly understood, hindering conservation efforts. Common snook, Centropomus undecimalis, depend on connected coastal habitats for reproduction and growth and can migrate extensively up rivers when not hindered by barriers to movement. In southern Mexico, they are frequently captured 300 river km or more upstream from the delta of the Usumacinta River. The Usumacinta is a large river system with no mainstem dams, providing an expansive network of connected river, stream and wetland habitats which form a much larger migratory domain that exists in many other systems where common snook have been studied. To assess whether fish captured in different zones were associated with different natal habitats or distinct migratory patterns, variation in common snook otolith chemical signatures was examined in the Usumacinta River from the Gulf of Mexico coast to as far as 600 river km upriver. Otolith microchemistry was useful for characterizing migratory histories of individuals, but there was no clear evidence that fish captured in different river zones used different types of natal habitats. Based on lifetime Sr:Ca in otoliths, a diverse array of movement patterns was evident in the Usumacinta system, with 97% of common snook showing evidence of freshwater habitat use. Prevalent use of riverine habitats by common snook far from the coast reinforces the need to preserve connectivity in the Usumacinta River and other undammed systems supporting migratory species. The natural flow regime and lack of barriers allow for longitudinal and lateral connectivity in this system, providing pathways for migratory species to move extensively and have access to an array of habitats, including productive floodplain lagoons.

Experimental warming and drying act independently on developmental responses for two amphibian species

AbstractClimate change is affecting freshwater habitats worldwide through multiple, interacting pressures that influence the survival, distribution, and persistence of freshwater organisms. Temporary freshwater habitats, or those that dry seasonally, can exhibit wide‐ranging variability in temperature and drying regimes. Aquatic organisms with complex life cycles (e.g., aquatic and terrestrial life stages) often rely on temporary freshwaters to complete their life cycles and have specific adaptations to cope with these dynamic environments. However, climate change is altering the temperature and drying regime of many temporary freshwater habitats worldwide, often resulting in warmer habitats available for shorter durations of time. The ways in which climate‐mediated changes to temporary freshwaters affect development and life history of aquatic organisms, particularly those with complex life cycles, remains poorly understood.We investigated larval development, growth, survival, and relationships among them for two amphibian species, wood frogs (Lithobates sylvaticus) and spring peepers (Pseudacris crucifer), in response to warming and drying in a mesocosm experiment. Over 13 weeks in the spring and summer of 2019, we manipulated temperature and water levels in 48 outdoor pond mesocosms to produce four treatments: control (ambient conditions), drying alone, warming alone, and drying + warming. We predicted reduced survival, body size, and time to metamorphosis for both species in response to warming and drying, with the effect being stronger on wood frogs due to their more specialised habitat use and shorter breeding period.Warming treatments were on average 2°C higher than controls, and drying treatments decreased water depth by 2.5 cm each week. We found that warming temperature shortened larval time to metamorphosis, and drying decreased body size at metamorphosis. Surprisingly, we also found that, across all treatment groups, individuals with early metamorphosis tended to be larger in size and from mesocosms with generally higher survival.Our study indicates that warming and drying may act independently on different developmental responses for amphibians. Our results echo recent calls for a better understanding of the nuanced, often unpredictable, relationships among climate‐mediated environmental pressures and the developmental traits they influence, particularly for organisms with complex life cycles that depend upon dynamic habitat for growth and survival. Understanding these relationships and the mechanisms that drive them will be key to predicting the response of freshwater organisms to climate change.

Variation in Larval Fish Assemblage Dynamics Associated with Harmful Algal Blooms in a Temperate Estuary, South Africa

Eutrophication and concomitant harmful algal blooms (HABs) are on the rise globally and pose a threat to larval stages of fishes that rely on estuarine nursery habitats for growth and survival. The anthropogenically altered low inflow estuary (LIE), Sundays Estuary, South Africa, supports persistent and predictable HABs. This study investigated the effects of HABs on the environmental conditions and larval fish assemblage of this warm temperate nursery area. Sampling took place during the austral spring of 2016 and 2018 at three sites in the mesohaline zone where both larval fish abundance and HABs are known to reach maxima. Physico-chemical and phytoplankton data were collected during the day and night, while larval fishes were sampled after nightfall. Physico-chemical parameters and larval fish assemblages were compared by water column (surface and bottom) and sites within the mesohaline zone, years, and HAB intensity (hypereutrophic ≥ 2781 H. akashiwo cells•mL−1; standard ≥ 205 H. akashiwo cells•mL−1; none < 205 H. akashiwo cells•mL−1). A longer period of consecutive hypereutrophic bloom conditions was recorded during 2018 compared to 2016. Dissolved oxygen concentration was notably higher during hypereutrophic blooms (χ2 = 23.759, df = 2, P < 0.001) and reached a maximum of approximately 21 mg•L−1 during the day and 13 mg•L−1 at night. Density and similarity of estuarine resident larval assemblages were negatively correlated to supersaturated dissolved oxygen concentrations. Greater mean densities of estuarine resident larvae were recorded during hypereutrophic blooms compared to standard blooms and bloom absence and diversity was lower during 2018 when hypereutrophic bloom conditions were more persistent. These changes may have major implications for successful early development of fishes that rely on the Sundays Estuary and similar LIEs as a nursery.

Active feeding of anadromous white-spotted char Salvelinus leucomaenis at the southern latitudinal rivers

Although starvation poses a serious risk of death, it is a common phenomenon among anadromous salmonids that fast after returning to the river following oceanic feeding migration. To address the effects of geographic environmental factors on their feeding, we examined the river feeding patterns and condition of sea-run migrants of white-spotted char ( Salvelinus leucomaenis) in rivers with latitudinal variation from 38°N to 46°N along the Sea of Japan. The river feeding patterns showed a significant latitudinal trend: char were observed to feed at lower latitudes and fast at higher latitudes. In contrast, the condition factor did not exhibit any latitudinal trends. These findings suggest that the environmental conditions encountered by individuals prior to and after river entry may influence their river feeding. Active feeding by the southern sea-run migrant char may be an adaptive strategy to maintain their body condition in response to the local conditions. This study highlights the importance of rivers not only as spawning and growth habitats for juveniles, but also as feeding habitats for certain anadromous salmonids.

Reassessing the origin of lentil cultivation in the Pre-Pottery Neolithic of Southwest Asia: new evidence from carbon isotope analysis at Gusir Höyük

Lentils represent the most common pulse crop found in Southwest Asian Neolithic archaeobotanical assemblages. Using carbon isotope determinations and palaeoclimatic modelling this paper investigates variability and change through time in lentil growth habitats at Gusir Höyük, a Pre-Pottery Neolithic habitation site in the Upper Tigris basin of southeast Anatolia dated to the 12th and 11th millennia cal bp. The elevated lentil carbon isotope values from samples dated to ~ 10,500–10,300 cal bp possibly points to greater water availability and the potential onset of lentil cultivation at Gusir Höyük during the Early Pre-Pottery Neolithic B. The paper revisits previous hypotheses about the process and timing of initial lentil domestication in the region through a re-evaluation of materials available from other Neolithic sites in SW Asia. Its findings highlight the important role of wild lentil populations growing in higher-rainfall localities, which may have responded more readily to increasing water availability in the context of early cultivation. It also explores the potential offered by isotopic approaches for detecting early cultivation, highlighting the need for future studies on pulse and cereal crop progenitors in SW Asia and beyond.

Regional differences in oceanic migratory behavior of Japanese silver eel in waters with different vertical temperature gradients

BackgroundThe Japanese eel Anguilla japonica has a single spawning area on the west coast of the Mariana Islands, and the larvae disperse widely via the North Equatorial Current and the Kuroshio Current to freshwater and coastal areas of East Asia for nursery and growth habitats. Spawning adults reared in their respective habitats begin their migration to spawn from September to February, but little is known about regional differences in migration routes, their contribution to spawning populations, or their migratory behavior. To better understand the spawning migrations of Japanese eels, we tracked adult migratory stage eels (silver eels) captured in coastal areas of three regions (Tokai region, Sea of Japan coastline, and Tohoku region) from October 2019 to February 2020 using pop-up satellite archival tags.ResultsTwenty-three tagged eels were released in each coastal area, and data were collected on 15 eels for a maximum of 59 days. Eels released in the Tokai region migrated southeast along the Kuroshio Current. On the other hand, eels on the Sea of Japan coastline and in the Tohoku region tended to migrate westward and southward against the ocean current, respectively, but no eels from either region reached waters near the Kuroshio Current. In addition, the present study found region-specific vertical movement patterns among the eels. Eels from the Tokai region showed typical diurnal vertical movements, while an eel from the Sea of Japan coastline repeated non-diurnal vertical movements, which was attributed to regional differences in bathymetry and water mass structure. Except for one eel suspected of being preyed upon, all other eels did not venture into depth zones with temperatures below 4 ^{circ }textrm{C}, thus suggesting that the maximum depth of vertical movement is limited by water temperature.ConclusionsThis study provided new insights into the migratory behavior of Japanese silver eel from the Sea of Japan coastline and the Tohoku region, but further long-term tracking is required to reveal the fate of the spawning adults from the coast of Japan.

Trees on the tundra: warmer climate might not favor prostrate Larix tree but Betula nana shrub growth in Siberian tundra (Lena River Delta)

Tundra is primarily a habitat for shrub growth, not trees, but growth of prostrate forms of trees has been reported occasionally from the subarctic tundra region. In the light of on-going climate change, climate sensitivity studies of these unique trees are essential to predict vegetation dynamics and potential northward expansion of boreal forest tree species into tundra. Here we studied one of the northernmost Larix Mill. trees and Betula nana L. shrubs (72°N) from the Siberian tundra for the common period 1980-2017. We took advantage of the discovery of a single cohort of prostrate Larix trees within a tundra ecosystem, i.e., ca. 60 km northwards from the northern treeline, and compared climate-growth relationships of the two species. Both woody plants were sensitive to the July temperature, however this relationship was stable across the entire study period (1980-2017) only for Betula nana chronology. Additionally, radial growth of Larix trees became negatively correlated to temperatures during the previous summer. In recent period moisture sensitivity between Larix trees and Betula nana shrubs was contrasting, with generally wetter soil conditions favoring Larix trees growth and dryer conditions promoting Betula nana growth. Our study indicates that Larix trees radial growth in recent years is more sensitive to moisture than to summer air temperatures, whereas temperature sensitivity of Betula nana shrub is stable over time. We provide first detailed insight into the annual resolution on Larix tree growth sensitivity to climate in the heart of the tundra. The potentially higher Betula nana shrub resistance to warmer and drier climate versus Larix trees on a tundra revealed in our study needs to be further examined across habitats of various soil, moisture and permafrost status.

AN ITS GENE-MEDIATED MOLECULAR DETECTION OF FUNGI ASSOCIATED WITH NATURAL AND ARTIFICIAL AGARWOOD FROM Aquilaria malaccensis

The current study examined fungal diversity in manufactured and natural agarwood samples perceived from A. malecensis trees in a plantation and the East Malaysia rainforest. Isolated fungi were also subjected to molecular analysis. The fungal community composition of healthy and damaged tree samples from both plantation and the wild forest was identified using PCR amplification of the internal transcribed spacer (ITS) region from fungal isolates. Meanwhile, ten groups of fungi isolates are expected to be placed in both natural and inoculated based on 1000 bootstrap values. They validated earlier genomic identification; 55 fungal isolates were discovered in artificial and natural agarwood, including agarwood and healthy wood. Ten fungal groupings were developed based on morphological characterization similarities. Based on NCBI BLAST analysis, molecular identification revealed Fusarium solani, Botryosphaeria theobromae, Polyporales spp, Schizophyllum commune, Aspergillus aculeatus, and Lasiodiplodia theobromae. Fusarium solani excelled in A. aculeatus and Polyporales spp. in agarwood samples and healthy wood. The presence of more fungi species in natural agarwood than in artificial agarwood may be due to the favourable humid and shaded habitat for fungal growth. Nonetheless, synthetic agarwood was able to synthesize agarwood-related chemical compounds. Considering F. solani was often identified in both agarwood, agarwood inoculant may act as a booster for F. solani to begin pathogenicity in artificial agarwood. The current research shows that artificial agarwood may provide quality comparable to natural agarwood and is not influenced by fungus interacting with the tree.

Rapid Adaptation of Chimonobambusa opienensis Leaves to Crown-Thinning in Giant Panda Ecological Corridor, Niba Mountain.

Leaf traits reflect the ecological strategy in heterogeneous contexts and are widely used to explore the adaption of plant species to environmental change. However, the knowledge of short-term effect of canopy management on understorey plant leaf traits is still limited. Here, we studied the short-term effect of crown-thinning on the leaf morphological traits of bamboo (Chimonobambusa opienensis), an important understorey plant and staple food for the giant panda (Ailuropoda melanoleuca) of Niba Mountain. Our treatments were two crown-thinnings (spruce plantation, CS, and deciduous broad-leaved forest, CB) and two controls (broad-leaved forest canopy, FC, and the bamboo grove of clearcutting, BC). The results showed that: the CS enhanced the annual leaf length, width, area, and thickness, CB decreased almost all annual leaf traits, and perennial leaf traits in CS and CB were the opposite. The log-transformed allometric relationships of length vs. width, biomass vs. area were significantly positive while those of specific leaf area vs. thickness were significantly negative, which varied largely in treatments and age. The leaf traits and allometric relationships suggested that the CS created a more suitable habitat for bamboo growth. This study highlighted that the understorey bamboo leaf traits could adapt the improved light environment induced by crown-thinning rapidly.