Plant Growth In Environments Research Articles

Inventory of the best-selling medicinal plants on the Lubumbashi markets (DR Congo) and authentication of samples from the 3 most popular species

Ethnopharmacological relevanceKnowledge of the high-sale medicinal plants and their authentication are essential parameters to ensure the safety of people using herbal medicine and to plan the safeguarding of medicinal species threatened with extinction. AimsThe present study, carried out in Lubumbashi, Democratic Republic of Congo, aimed to geolocate medicinal plant sales points, list the best-selling species, and authenticate samples of the most popular species on the market. MethodsA survey was conducted among the medicinal plant sellers in Lubumbashi’s markets and other public spaces, to identify the best-selling species. Samples of the species reported as the most sold were purchased for sales unit weight measurement, and authentication. For the 3 most popular plant species, the identity of 92 samples, purchased from some 25 herbalists, was assessed by combining the microscopic characteristics of powdered drugs with the HPTLC fingerprints of methanolic extracts; for each species, these samples were compared with 1 or 2 botanically authenticated reference samples. As abundant starch granules were detected by microscopy, some samples were suspected of heavy flour contamination, which was confirmed by an enzymatic determination of their starch content. ResultsA total of 108 herbalists (48.1 % women) with a median age of 37 years (range, 20 to 67 years), and a median seniority of 5 years (0.7 to 30) were interviewed. From a total of 514 purchased samples, 396 (77.0 %) corresponded to 56 species that could be identified, including 92 samples representing the 3 most frequently sold plant species. The identities of 118 samples (having 82 different vernacular names), could not be determined, due to the lack of voucher specimen Roots were the most sold organ (56.0 %; n = 514), mainly in powder form (78.7 %), and the median price was 21 USD/kg, at the time of the survey (01/2022). The identified specimens were, predominantly, the roots of Terminalia mollis M.A.Lawson (33.3 %), Securidaca longepedunculata Fresen (28.7 %), and stem barks of Nauclea pobeguinii Hua ex Pobég. (23.1 %); from the recorded sales figures, the amounts of material annually sold for these 3 species are estimated at about 5.7, 6.0, and 3.1 tons, respectively. Some sellers reported problems in identifying and/or preserving S. longepedonculata and T. mollis.Among the 92 samples analyzed for the three species, 18.5 % raised problems, including species substitutions (14/17), dilution with flour (1/17), dilution with flour and species substitution (1/17), and detection of a phytochemical variant (1/17), that could arise either from contamination by another species, a particular plant growth environment, or poor storage conditions. ConclusionThe confusion/adulteration rate measured here for highly popular species is quite alarming (18.5 %); but, indeed, roots, especially as powders, are often difficult to differentiate based on the coarse organoleptic examination practiced by herbal traders. Microscopic and phytochemical characteristics reported in this study, quite easy to obtain with basic laboratory equipment, should be systematically applied by the health products regulatory authorities to control the quality of herbals and ensure that patients get the drug desired for their treatment.

Cfd-based optimization of Direct evaporative cooling systems for canarian greenhouses in Semi-Arid regions

Direct Evaporative Cooling Systems (DECS) offer a promising solution for mitigating heat stress in greenhouses located in arid and semi-arid regins. To implement DECS efficiently in these regions, it is crucial to understand their impact on greenhouse microclimate parameters. This study investigates the influence of DECS on the microclimate within a canarian greenhouse and identifies key factors affecting their efficiency. Through Computational Fluid Dynamics (CFD) simulations using Ansys, we examined the effects of DECS on temperature, relative humidity, and air velocity within the greenhouse under semi-arid conditions.The results indicate that DECS efficiency is affected by several factors, including fan placement, evaporative pad height, fan spacing, and inlet air fan speed. Higher fan placement cools the upper canopy but increases humidity near the ceiling, whereas lower placement improves temperature uniformity but may require additional humidification. Closer fan spacing results in uneven air velocity, while wider spacing leads to stagnant zones within the greenhouse. Optimal placement of the the evaporative pad is crucial for maximizing the cooling coverage area and minimizes the temperature gradients. Inlet air fan speed also significantly impacts the microclimate, with higher speeds reducing both temperature and humidity. By optimizing these parameters, the DECS system was able to significantly decrease air temperature, increase humidity, and improve air velocity inside the greenhouse. With a pad height of 1.5 m, fans positioned at a height of 2 m, a distance of 6 m between each fan, and an air speed of 1.5 m/s at the entrance – the DECS enhances the local climate. Under these conditions, the mean air temperature drops from 40°C −in greenhouse with natural ventilation- to 21 °C using DECS, while relative humidity increases from 23 % −in greenhouse with natural ventilation- to 67 % using DECS, creating a more favorable environment for plant growth and potentially increasing crop yield and quality. Additionally, energy and environmental analyses show that implementing DECS in Canarian greenhouses, instead of traditional electrical cooling systems, results in substantial energy savings of 123,818.112 kWh/year annually and reduces carbon dioxide emissions by approximately 1.785 tons/year over a 20-year operational lifespan in a semi-arid climate.

Performance study of solar air collector-air source heat pump system inside the greenhouse

During the process of heating greenhouses, the phenomenon of inadequate heating installations is widespread. To address this issue, this paper proposed a solar air collector-air source heat pump (SAC-ASHP) system for greenhouses, combining solar technology with heat pump (HP) technology to provide sufficient heat during continuous cloudy days and winter. The heating capacities of the system on sunny, cloudy, and overcast days were 100.2 kWh, 112.3 kWh, and 157.8 kWh, respectively. The corresponding power was 30.1 kWh, 36.1 kWh, and 53.6 kWh. The coefficient of performance (COP) of the single air source heat pump (ASHP) for these days was 3.4, 3.2, and 3.0, respectively, while the COP of the solar heat pump (SHP) was 4.0, 3.5, and 3.1. Additionally, greenhouse employed heat and moisture recovery for humid air, aiming to provide a suitable environment for plant growth around the clock while conserving energy. The heat collection in the heat and moisture recovery combined HP mode was 66.7 % higher than in the heat exchanger (HE)-fan mode. This experimental study investigated the operational performance of the system in dynamic environments, revealing the stability of the system for greenhouse heating in cold regions, and providing new research ideas for greenhouse heating.

Soil Bacteria from the Namib Desert: Insights into Plant Growth Promotion and Osmotolerance in a Hyper-Arid Environment

The Namib Desert is characterized by a number of abiotic stresses, including high temperature, high salinity, osmotic pressure, alkaline pH, and limited water availability. In such environments, dry soils typically exhibit a low water potential, scarce nutrients, and high concentrations of dissolved ions, collectively creating a challenging habitat for microbial life. In this study, 89 bacterial isolates belonging to 20 genera were identified. Bacteria demonstrated significant osmotolerance, with some strains thriving at polyethylene glycol (PEG) concentrations exceeding 20%. Furthermore, these bacteria demonstrated halotolerance, high pH tolerance, and capacity to produce plant growth-promoting (PGP) traits under conditions of osmotic stress. Osmotolerant bacteria exhibited higher proficiency in siderophore production, potassium solubilization, and phosphorus solubilization, all of which are critical for supporting plant growth in nutrient-scarce and stressful environments, such as deserts. However, alginate production was higher in isolates that were less osmotolerant, indicating the potential for a compensatory mechanism in strains that were more sensitive. These findings highlight the complex strategies employed by desert bacteria to survive and support host plants in extreme environments. The present study not only enhances our understanding of microbial adaptations in arid ecosystems, but also provides important information for the development of potential applications for these bacteria in the reclamation of arid land and agricultural practices aimed at improving crop resilience to abiotic stress.

Unveiling the Hidden Responses: Metagenomic Insights into Dwarf Bamboo (Fargesia denudata) Rhizosphere under Drought and Nitrogen Challenges.

Dwarf bamboo (Fargesia denudata) is a crucial food source for the giant pandas. With its shallow root system and rapid growth, dwarf bamboo is highly sensitive to drought stress and nitrogen deposition, both major concerns of global climate change affecting plant growth and rhizosphere environments. However, few reports address the response mechanisms of the dwarf bamboo rhizosphere environment to these two factors. Therefore, this study investigated the effects of drought stress and nitrogen deposition on the physicochemical properties and microbial community composition of the arrow bamboo rhizosphere soil, using metagenomic sequencing to analyze functional genes involved in carbon and nitrogen cycles. Both drought stress and nitrogen deposition significantly altered the soil nutrient content, but their combination had no significant impact on these indicators. Nitrogen deposition increased the relative abundance of the microbial functional gene nrfA, while decreasing the abundances of nirK, nosZ, norB, and nifH. Drought stress inhibited the functional genes of key microbial enzymes involved in starch and sucrose metabolism, but promoted those involved in galactose metabolism, inositol phosphate metabolism, and hemicellulose degradation. NO3--N showed the highest correlation with N-cycling functional genes (p < 0.01). Total C and total N had the greatest impact on the relative abundance of key enzyme functional genes involved in carbon degradation. This research provides theoretical and technical references for the sustainable management and conservation of dwarf bamboo forests in giant panda habitats under global climate change.

Exploring the influence of polymers on soil ecosystems: prospective from agricultural contexts

The utilization of advanced polymeric materials has indeed emerged as a significant trend in sustainable agriculture, offering a range of innovative applications aimed at enhancing productivity, minimizing environmental impact, and promoting resource efficiency. Smart polymeric materials enable the controlled release of pesticides, herbicides, and fertilizers, thereby enhancing their efficacy while reducing the quantities needed. Superabsorbent polymeric materials act as soil conditioners, assisting in alleviating the negative impacts of drought by retaining moisture and enhancing soil structure. This fosters improved plant growth and resilience in water-scarce environments. Polycationic polymers play a role in plant bioengineering, facilitating genetic transformation processes aimed at enhancing crop productivity and disease resistance. Advanced polymeric systems contribute to the arsenal of precision agriculture tools by enabling precise delivery and targeted application of agricultural inputs. This approach enhances resource efficiency, reduces waste, and minimizes environmental impact while optimizing crop yields. In reviewing recent developments in the design and application of advanced polymeric systems for precision agriculture, several key considerations emerge.

Plant Beneficial Desert Actinomycete, Modestobacter caceresii KNN 45-2bT, Promote Growth of Tomato (Lycopersicum esculentum Mill.) under Drought Condition

Drought stress is currently the most serious challenge to global food security and agricultural productivity. Desert actinobacteria have gained attention as potential candidates for enhancing plant growth in water stress environments. In this regard, a desert actinomycete, namely Modestobacter caceresii strain KNN 45-2bT, was selected to investigate its plant growth promoting abilities and drought tolerance. Next, this desert strain was inoculated to tomato (Lycopersicum esculentum Mill.) under drought, and the results included increases in root length, shoot and root fresh weight, shoot and root dry weight, total fresh weight and dry weight, fruit weight, proline accumulation, total soluble sugar content and trolox content. Stress treatments on the bacterized tomato plants also resulted in the reduction of hydrogen peroxide accumulation. Putative proteins coding sequences conferring plant growth promoting (PGP) traits (IAA production, phosphate solubilization, siderophore production, nitrogen fixation) and drought response (biosynthesis of proline metabolism, oxidative and osmotic stress response) were also detected from their genomic analyses. In conclusion, these results provide credence to the idea that the inoculation of tomato plants with plant beneficial desert actinomycete is an effective method of combating the negative effects of drought stress.

Genetic diversity, stress tolerance and phytobeneficial potential in rhizobacteria of Vachellia tortilis subsp. raddiana

BackgroundSoil bacteria often form close associations with their host plants, particularly within the root compartment, playing a significant role in plant growth and stress resilience. Vachellia tortilis subsp. raddiana, (V. tortilis subsp. raddiana)a leguminous tree, naturally thrives in the harsh, arid climate of the Guelmim region in southern Morocco. This study aims to explore the diversity and potential plant growth-promoting (PGP) activities of bacteria associated with this tree.ResultsA total of 152 bacterial isolates were obtained from the rhizosphere of V. tortilis subsp. raddiana. Rep-PCR fingerprinting revealed 25 distinct genomic groups, leading to the selection of 84 representative strains for further molecular identification via 16 S rRNA gene sequencing. Seventeen genera were identified, with Bacillus and Pseudomonas being predominant. Bacillus strains demonstrated significant tolerance to water stress (up to 30% PEG), while Pseudomonas strains showed high salinity tolerance (up to 14% NaCl). In vitro studies indicated variability in PGP activities among the strains, including mineral solubilization, biological nitrogen fixation, ACC deaminase activity, and production of auxin, siderophores, ammonia, lytic enzymes, and HCN. Three elite strains were selected for greenhouse inoculation trials with V. tortilis subsp. raddiana. Strain LMR725 notably enhanced various plant growth parameters compared to uninoculated control plants.ConclusionsThe findings underscore the potential of Bacillus and Pseudomonas strains as biofertilizers, with strain LMR725 showing particular promise in enhancing the growth of V. tortilis subsp. raddiana. This strain emerges as a strong candidate for biofertilizer formulation aimed at improving plant growth and resilience in arid environments.

THE PERSPECTIVITIES OF MICRICLONAL PROPAGATION OF ACER PLATANOIDES L. CRIMSON KING (ACEARCEAE JUSS.)

Clonal micropropagation – massive asexual propagation of plants from cell and tissue culture; the resulting plants are genetically identical to the original copy. The conservation of plants by tissue culture methods is of great interest and will probably become even more common in the future. The advantages of this method are numerous, but we find it most important that the initiation of cultivation requires only a single plant, a seed or a single explant (apex, meristem, bud, piece of leaf, stem etc.), thus, the available plant specimens (which can be only a few) will not be affected by harvesting them from their habitat. The state program “Developing of the scientific basis for the sustainable reproduction of valuable specimens of the botanical garden collection in in vitro culture” is being conducted in the laboratory of biotechnology of the Tashkent Botanical Garden of the Institute of Botany, Academy of Sciences of the Republic of Uzbekistan, directed at microclonal propagation of many valuable species presented in a single copy, as well as species in demand in urban greening programs. Acer platanoides L. Crimson King variety (Acearceae Juss.) is a deciduous, slow-growing tree, reaching 15 m with age. The crown shape is wide-round and even. The leaves are large, 5-7 lobed, deep purple, and do not change color throughout the growing season. The trunk is smooth, dark in color, with clearly defined longitudinal grooves. Because of its graceful shape, the Crimson King variety is extremely popular. It is actively planted in city yards, parks, and roadsides. Suitable for landscaping. Within this state program the protocol of microclonal propagation of A. platanoides Crimson King variety has been developed to propagate this species for Botanical Garden collection as well as for greening programs. Solutions of sodium hypochloride (4-6%), hydrogen peroxide (2-15%), silver nitrate (0.01%), Tween20, ethanol (70%), and some other sterilizing agents were tested for developing a protocol for sterilization of maple explants. “Belizna” solution, “Domestos” sterilizing soap, fungicides difenoconazole, mancoseb and metalaxyl, fludioxonil, propiconazole, antibiotics streptomycin, amoxicillin, gentamicin, and etc. were also implemented for explants sterilization. As a result of the studies, it was determined that the most optimal source of explants are branches with apical (10% regeneration) and lateral (100% regeneration) buds of the annual shoot of A. platanoides Crimson King variety. Plant growth environment is important in the success of in vitro propagation. Thus, explants obtained from trees growing in the arid areas of massive Kuylyuk in Tashkent, were characterized by low viability and a high degree of infection with fungal diseases in comparison with explants obtained from trees growing in Hastimom Square in Tashkent, where trees are watered daily. We found two ways of in vitro propagation of A. platanoides L. variety Crimson King: 1. With the use of microcuttings. 2. Indirect organogenesis with induction of callus. The best way for A. platanoides L. variety Crimson King became indirect organogenesis trough induction of the callus in the WPM - Wood Propagation Media, hormone free or with 6- benzilaminopurine in the media. The duration of the developing of a plant usually takes 6-9 months.

Effects of Shading Nets Color on the Internal Environmental Conditions, Light Spectral Distribution, and Strawberry Growth and Yield in Greenhouses.

Greenhouses are used to create the appropriate environment for plant growth. Controlling the level of lighting using shading nets is one of the most commonly used methods for making suitable environmental modifications in greenhouses. The objective of this study was to examine the impact of three colored shading nets (green, black, and beige at shading rates of 50%) on inside air temperature, relative humidity, and spectral distribution of light in a greenhouse, as well as their effect on the growth and yield of strawberry plants. Data were collected during winter (December and January) and spring (March and April) months from shaded and unshaded blocks. The green net had the highest transmittance to solar radiation (τSR) during the two periods (38% and 35%, respectively) and the highest transmittance to photosynthetically active radiation (τPAR) of 34% during spring months, while the beige net had the highest τPAR of 27% during winter months. The black net had the smallest τPAR values during the two periods (22% and 29%, respectively). The lowest total light levels per season for solar radiation (SR) and photosynthetically active radiation (PAR) (746.8 and 293.7 MJ·m-2, respectively) were obtained under the black net, compared with (906.7 and 320.8 MJ·m-2, respectively) for the beige net, and (969.6 and 337.2 MJ·m-2, respectively) for the green net. The ratio of PAR to SR (PAR:SR) was 41% and 44% outside and inside the greenhouse for the control (without shade), respectively. The black net had the highest ratio of PAR:SR (39%) among the treatment nets. The green net transmitted more light in the blue-green region (400 to 570 nm) and transmitted the highest photon flux at 480 nm, while the beige net increased the infrared radiation flux from 730 nm and above and transmitted the highest photon flux at 604 nm. The study found that the green net increased the ratio of blue to red light (B/R), while the beige and green nets reduced the red to far-red light (R/FR) ratio. The photosynthetic rate, conductance to water, and transpiration were significantly higher for strawberries grown under the beige net. These results indicate that the beige net positively influenced leaf and stem characteristics, leading to improved strawberry yields. The best yields of strawberries were obtained under the beige net and the control group (no shade), surpassing the yields achieved under the black net by 26.3% and 21.4%, respectively.

Dicranopteris dichotoma rhizosphere-derived Bacillus sp. MQB12 acts as an enhancer of plant growth via increasing phosphorus utilization, hormone synthesis, and rhizosphere microbial abundance

In recent years, microbial inoculants have showed a great potential to replace chemical fertilizers as a new generation of soil amendment agents, however, the understanding of their effects on nutrient cycling within plants and rhizosphere microbial diversity are still limited. In this study, the rhizosphere growth-promoting bacteria MQB12 was used to inoculate Vigna radiata to evaluate the effects of external inoculants on plant transcriptomics and rhizosphere soil microbial diversity. Enrichment analysis using GO and KEGG revealed significant enrichment in DNA-binding transcription factor activity, transcriptional regulatory factor activity, and phenylpropanoid biosynthesis among the differentially expressed genes. MQB12 inoculation positively responded to phosphorus starvation response, increased the expression of phosphorus starvation response genes (PHT/PAP), enhanced the synthesis of ethylene and salicylic acid to cope with external stress, and improved the expression of plant disease resistance genes to strengthen the disease resistance of plants to pathogens. At the same time, microbial diversity analysis further revealed the positive effect of MQB12 inoculum. MQB12 inoculum enriched beneficial flora, improved flora abundance, changed the structure and diversity of V. radiata rhizosphere microbial community, enhanced the interconnections between the flora, and positively promoted growth. MQB12 was found to adjust the microflora of the rhizosphere, which subsequently changed the environment for plant colonization. This change led to the enrichment of beneficial bacteria and removal of pathogenic bacteria, which positively affected the internal pathways of plants. Additionally, changes in gene expression levels of plants resulted in the formation of different phenotypes and various metabolites, further influencing the formation of rhizosphere microbial communities through close contact between roots and soil. This study provides new insights into the effects of microbial agents on plant growth and root environment construction and is conducive to the further development and application of microbial agents.Graphical

The adsorption and fixation of Cd and Pb by the microbial consortium weakened the toxic effect of heavy metal-contaminated soil on rice

With the rapid development of industry and agriculture, the toxicity and pollution of Cd and Pb in soil and plants are becoming increasingly severe. Microbial remediation is considered an economical and sustainable method for addressing heavy metal ion pollution. This experiment established a microbial consortium capable of efficiently adsorbing and fixing Cd and Pb. The consortium consisted of two isolated strains: the straw-degrading bacterium ZJW-6 (Cellulomonas iranensis) and the phosphate-solubilizing bacterium wj1 (Pseudomonas brassicacearum). The microbial consortium exhibited high resistance to Cd and Pb, resulting in a significant reduction in heavy metal toxicity. The minimum inhibitory concentrations of Cd and Pb for the mixed microbial consortium were 900 mg/L and 2500 mg/L, respectively. Results indicated that within seven days, the microbial consortium’s removal rates of Cd and Pb reached 94.25 % and 74 %, respectively, surpassing the removal abilities of the individual member bacteria. It also enriched the soil organic matter and produced larger aggregates to adsorb more heavy metals (Cd: 3.35 mg/kg, Pb: 86.57 mg/kg). By the 60th day, 91.2 % of Cd and 97.89 % of Pb in the soil rhizosphere had been removed, and the free Cd and Pb in plant tissues were significantly reduced. Soil aggregates, which are the basic units of soil and an important index for measuring soil structure, were improved. The microbial consortium also played a significant role in restoring the soil microbial community, mobilizing the combined action of microorganisms represented by Ramlibacter and Sphingomonas to repair heavy metal pollution and promote plant growth. This provides a new method to bioremediate metal-contaminated soil and enhance the plant growth environment.

Solar arrays create novel environments that uniquely alter plant responses

Societal Impact StatementGlobally, the combustion of fossil fuels represents the vast majority of greenhouse gas emissions, and as such, a transition to renewable forms of energy provides the greatest potential for mitigating climate warming. Although solar photovoltaic energy generation is a leading climate solution, these energy facilities have a significant spatial footprint. Naturally, concerns regarding the coexistence of solar development in agriculturally productive and pristine native ecosystems remain. This study offers insight for how plants respond to novel environmental conditions within a solar array and contextualizes results to inform future array siting, design, and management to realize a sustainable solar energy future.Summary Photovoltaic (PV) solar arrays impose dynamic shading regimes and redistribute precipitation to the ecosystems beneath, leading to spatial and temporal heterogeneity in plant growth environments. Although PV are known to alter ecosystem‐level processes in managed and native landscapes, the control of PV‐induced microenvironments on plant ecophysiological responses are largely unexplored. A more robust and mechanistic understanding of how PV microenvironments control plant response will inform management of existing solar arrays and provide insight for future arrays designed to enhance ecosystem services. Here, we evaluated carbon (photosynthetic parameters) and water relations (daily patterns of leaf water potential (ψL) and stomatal conductance (gsw)) in a C3 perennial grass (Bromus inermis) across PV microsites within a 1.6 ha (1.2 MW) array in semiarid Colorado, USA. Light‐saturated photosynthetic rate was surprisingly consistent spatially, not differing between plants growing in near full sun (between PV rows) versus those growing in shadier microsites beneath panels (~28% of full sunlight). Additionally, plants located in microsites receiving only direct sunlight in the morning, when air temperature and vapor pressure deficits (VPD) were low, had greater ψL and gsw than plants receiving direct sunlight primarily in the hotter drier afternoon. Thus, while soil moisture is a primary control of plant productivity in most water‐limited grasslands, we found that VPD was a better predictor of daily patterns of leaf‐level photosynthetic and water relations responses that control aboveground biomass production in a PV array. These findings provide new mechanistic insight for evaluating vegetation management strategies in semiarid PV arrays.

Next generation plant biostimulants & genome sequencing strategies for sustainable agriculture development.

The best environment for plant growth and development contains certain essential metabolites. A broad category of metabolites known as "plant biostimulants" (PBs) includes biomolecules such as proteins, carbohydrates, lipids, and other secondary metabolites related to groups of terpenes, specific nitrogen-containing compounds, and benzene ring-conjugated compounds. The formation of biomolecules depends on both biotic and abiotic factors, such as the release of PB by plants, animals, and microorganisms, or it can result from the control of temperature, humidity, and pressure in the atmosphere, in the case of humic substances (HSs). Understanding the genomic outputs of the concerned organism (may be plants or others than them) becomes crucial for identifying the underlying behaviors that lead to the synthesis of these complex compounds. For the purposes of achieving the objectives of sustainable agriculture, detailed research on PBs is essential because they aid in increasing yield and other growth patterns of agro-economic crops. The regulation of homeostasis in the plant-soil-microbe system for the survival of humans and other animals is mediated by the action of plant biostimulants, as considered essential for the growth of plants. The genomic size and gene operons for functional and regulation control have so far been revealed through technological implementations, but important gene annotations are still lacking, causing a delay in revealing the information. Next-generation sequencing techniques, such as nanopore, nanoball, and Illumina, are essential in troubleshooting the information gaps. These technical advancements have greatly expanded the candidate gene openings. The secondary metabolites being important precursors need to be studied in a much wider scale for accurate calculations of biochemical reactions, taking place inside and outside the synthesized living cell. The present review highlights the sequencing techniques to provide a foundation of opportunity generation for agricultural sustainability.

Kosakonia arachidis: Shielding Vigna radiata in metal-stressed Hydroponics

The process of modernization and industrialization has indeed heightened environmental toxicity. Deterioration of plant growth and nutrient uptake has resulted in adverse effects on human health. Heavy metals are the most prominent pollutants. Elevated levels of heavy metals can surpass essential elements, leading to deficiencies in plant nutrition and hindering growth. The objective of the study was to extract bacteria that are resistant to zinc and cadmium from fields located in close proximity to the Malviya Nagar industrial zone in Jaipur. After collecting a soil sample, colonies were isolated on nutrient agar medium with varying amounts of heavy metals. Characterization of well-defined colonies was conducted using morphological and biochemical methods, and identification of resistant bacteria was performed using advanced bacterial isolation software (ABIS). Azohydromonas lata, Kosakonia arachidis, Pseudomonas fluorescens, and Pseudomonas aeruginosa were the prominent bacterial species found to have a similarity value over 90%. Among all the bacteria that were identified, Kosakonia arachidis was selected for additional investigation due to the scarcity of studies conducted on this bacterium for the purpose of mitigating heavy metal contamination from soil. The study aimed to assess its capacity to enhance the development of mung beans in non-circulating hydroponic systems under zinc and cadmium stress conditions. The presented study demonstrates the remarkable capacity of Kosakonia arachidis to facilitate plant growth in environments characterized by zinc concentrations of 1000 ppm and cadmium concentrations of 300 ppm. This characteristic renders it a highly promising and pragmatic choice for biofertilizer utilization in plants experiencing stress caused by heavy metals.

A dataset of desert plant catalogue in Xinjiang

The dataset of desert flora catalogue in Xinjiang was constructed by consulting monographs such as Chinese Flora, Xinjiang Flora, Chinese Desert Flora, Xinjiang Flora, Xinjiang Tree Chronicles, as well as related documents and major online open databases, aiming to enhance the accuracy and completeness of the data and information included in the catalogue. This dataset comprises 31 families, 150 genera and 360 species of desert plants in Xinjiang, including 355 species of angiosperms and 5 species of gymnosperms. Among them, there are 80 species of plants with medicinal value, 61 species of plants with forage value, and 26 species with economic value, accounting for 22.2%, 16.9% and 7.2% of all plants in the dataset, respectively. This dataset contains the Chinese names, Latin names, aliases and other information of desert plants, which can provide scientific reference and basic data for scientific research on various aspects, such as the growth environment and plant value of desert plants. Moreover, it is of great significance for the conservation and development of desert plant diversity in Xinjiang.

Agricultural light-converting anti-icing superhydrophobic coating for plant growth promotion

Agricultural coatings play a crucial role in addressing the global food crisis by significantly improving the plant growth environment, accelerating plant development, and enhancing the biological quality of plants. Nevertheless, there exist constraints in the form of limited sunlight utilization, decreased light transmission caused by surface icing leading to diminished plant photosynthesis, and high cost. In this study, a cost-effective and readily scalable superhydrophobic photothermal light-conversion coating is suggested as a means to improve the light conditions necessary for optimal plant growth. By employing in situ growth of carbon dots (CDs) on the surface and interlayers of montmorillonite (MMT) and hydrolytic polymerization of fluorinated alkyl silane (FAS) on the surface of MMT, light-converting superhydrophobic materials (CDs/MMT) with a micro-nano structure were synthesized. These materials demonstrated broad-spectrum fluorescence emission suitable for utilization by plant photosynthesis. They were dispersed into an epoxy resin (ER) matrix to obtain an agricultural superhydrophobic coating (ER-CDs/MMT). The “light absorption-capture-conversion” effect confers exceptional passive anti-icing and active de-icing properties upon the coating. Furthermore, outdoor planting experiments have demonstrated its capacity to substantially enhance the light environment and enhance the biological quality of soybean and morning glory by leveraging light scattering and conversion characteristics. This underscores its considerable potential for application in the next generation of facility agriculture.

Effectiveness of the Fuzzy Logic Control to Manage the Microclimate Inside a Smart Insulated Greenhouse

Agricultural greenhouses incorporate intricate systems to regulate the internal climate. Among the crucial climatic variables, indoor temperature and humidity take precedence in establishing an optimal environment for plant production and growth. The present research emphasizes the efficacy of employing intelligent control systems in the automation of the indoor climate for smart insulated greenhouses (SIGs), utilizing a fuzzy logic controller (FLC). This paper proposes the use of an FLC to reduce the energy consumption of a greenhouse. In the first step, a thermodynamic model is presented and experimentally validated based on thermal heat exchanges between the indoor and outdoor climatic variables. The outcomes show the effectiveness of the proposed model in controlling indoor air temperature and relative humidity with a low error percentage. Secondly, several fuzzy logic control models have been developed to regulate the indoor temperature and humidity for cold and hot periods. The results show the good performance of the proposed FLC model as highlighted by the statistical analysis. In fact, the root mean squared error (RMSE) is very small and equal to 0.69% for temperature and 0.23% for humidity, whereas the efficiency factor (EF) of the fuzzy logic control is equal to 99.35% for temperature control and 99.86% for humidity control.

Diversity and Plant Growth Properties of Rhizospheric Bacteria Associated with Medicinal Plants.

Microbes in the rhizosphere play a significant role in the growth, development, and efficiency of plants and trees. The rhizospheric area's microbes are reliant on the soil's characteristics and the substances that the plants release. The majority of previous research on medicinal plants concentrated on their bioactive phytochemicals, but this is changing now that it is understood that a large proportion of phytotherapeutic substances are actually created by related microorganisms or through contact with their host. The roots of medicinal plants secrete a large number of secondary metabolites that determine the diversity of microbial communities in their rhizosphere. The dominant bacteria isolated from a variety of medicinal plants include various species of Bacillus,Rhizobium,Pseudomonas,Azotobacter,Burkholderia,Enterobacte,Microbacterium, Serratia,Burkholderia, andBeijerinckia. Actinobacteria also colonize the rhizosphere of medicinal plants that release low molecular weight organic solute that facilitate the solubilisation of inorganic phosphate. Root exudates of medicinal plants resist abiotic stress and accumulate in soil to produce autotoxic effects that exhibit strong obstacles to continuous cropping. Although having a vast bioresource that may be used in agriculture and modern medicine, medicinal plants' microbiomes are largely unknown. The purpose of this review is to (i) Present new insights into the plant microbiome with a focus on medicinal plants, (ii) Provide information about the components of medicinal plants derived from plants and microbes, and (iii) Discuss options for promoting plant growth and protecting plants for commercial cultivation of medicinal plants. The scientific community has paid a lot of attention to the use of rhizobacteria, particularly plant growth-promoting rhizobacteria (PGPR), as an alternative to chemical pesticides. By a variety of processes, these rhizobacteria support plant growth, manage plant pests, and foster resilience to a range of abiotic challenges. It also focuses on how PGPR inoculation affects plant growth and survival in stressful environments.

Adaptation mechanism of three Impatiens species to different habitats based on stem morphology, lignin and MYB4 gene

BackgroundImpatiens is an important genus with rich species of garden plants, and its distribution is extremely extensive, which is reflected in its diverse ecological environment. However, the specific mechanisms of Impatiens’ adaptation to various environments and the mechanism related to lignin remain unclear.ResultsThree representative Impatiens species,Impatiens chlorosepala (wet, low degree of lignification), Impatiens uliginosa (aquatic, moderate degree of lignification) and Impatiens rubrostriata (terrestrial, high degree of lignification), were selected and analyzed for their anatomical structures, lignin content and composition, and lignin-related gene expression. There are significant differences in anatomical parameters among the stems of three Impatiens species, and the anatomical structure is consistent with the determination results of lignin content. Furthermore, the thickness of the xylem and cell walls, as well as the ratio of cell wall thickness to stem diameter have a strong correlation with lignin content. The anatomical structure and degree of lignification in Impatiens can be attributed to the plant's growth environment, morphology, and growth rate. Our analysis of lignin-related genes revealed a negative correlation between the MYB4 gene and lignin content. The MYB4 gene may control the lignin synthesis in Impatiens by controlling the structural genes involved in the lignin synthesis pathway, such as HCT, C3H, and COMT. Nonetheless, the regulation pathway differs between species of Impatiens.ConclusionsThis study demonstrated consistency between the stem anatomy of Impatiens and the results obtained from lignin content and composition analyses. It is speculated that MYB4 negatively regulates the lignin synthesis in the stems of three Impatiens species by regulating the expression of structural genes, and its regulation mechanism appears to vary across different Impatiens species. This study analyses the variations among different Impatiens plants in diverse habitats, and can guide further molecular investigations of lignin biosynthesis in Impatiens.