Growth Capacity Research Articles

MaEng1, an endo-1,3-glucanase, contributes to the conidiation pattern shift through changing the cell wall structure in Metarhizium acridum

Microcycle conidiation has displayed the greater potential than normal conidiation in large-scale production of mycopesticides. Fungi require partial hydrolysis of the cell wall to achieve the necessary plasticity during their morphological changes. Therefore, various cell wall-associated hydrolases are crucial for fungal morphogenesis. Eng1, as an endo-β-1,3-glucanase, is involved in the cell separation of fungi, but its role in morphological changes of entomopathogenic fungi is not yet clear. Here, the endo-β-1,3-glucanase gene MaEng1 was characterized in the model entomopathogenic fungi M. acridum. MaEng1 possesses a typical carbohydrate hydrolase domain and belongs to the GH81 family. The functions of MaEng1 in fungal growth, stress tolerance, pathogenicity, and conidiation capacity were analyzed using targeted gene disruption. The results displayed that the absence of MaEng1 does not affect the fungal growth, stress tolerances, and pathogenicity in M. acridum. However, the knockout of MaEng1 led to the normal conidiation of M. acridum on the SYA medium, which can induce the microcycle conidiation. Moreover, the content of β-1,3-glucan in the cell wall of the MaEng1-disruption strain were significantly reduced and the exposures of β-1,3-glucan on the surface of the mature conidia and mycelia in ΔMaEng1 were declined, indicating that MaEng1 contributes to the conversion of conidiation mode in M. acridum by affecting the cell wall structure.

Growth and Reproduction Performance of the Earthworm (Eudrilus eugeniae) Fed with Different Substrates Made of Palm Oil Empty Fruit Bunches: A Case of Vermicompost Production, Abidjan, Côte d'Ivoire

The objective of the present study is to analyze the growth capacity and activity of the worm species Eudrilus eugeniae in different substrates based on palm oil empty fruit bunches as part of the production of vermicompost. To this end, 4 treatments were carried out by adding to palm oil empty fruit bunches other substrates readily available in the environment, notably Chromolaena odorata leaves and grass clippings. 1.5 L plastic boxes were filled with 800 g of soil and 300 g of various precomposted substrates. In each box, 4 earthworms (Eudrilus eugeniae) were seeded. A total of 4 treatments were carried out: T1 (palm oil empty fruit bunches + earthworms), T2 (palm oil empty fruit bunches + Chromolaena odorata + earthworms), T3 (palm oil empty fruit bunches + grass clippings + earthworms) and T4 (palm oil empty fruit bunches + Chromolaena odorata + grass clippings + earthworms). For the different vermicomposts based on palm oil empty fruit bunches, growth parameters and Eudrilus eugeniae activity were better in treatments T3 and T4. These same treatments also showed significant variation in substrate quantity after vermicomposting. The combination of palm oil empty fruit bunches and green waste (Chromolaena odorata and grass clippings) would be ideal for the production of vermicompost with good agronomic potential to be applied as an organic amendment to cultivated plants.

Stem cell seeding of decellularised porcine right ventricular outflow tracts to advance repair of congenital heart defects

Abstract Background Replacement of the right ventricular outflow tract (RVOT) is necessary in many critical congenital heart disease subtypes, often when the child is less than one year old. Poor availability of small-diameter cardiac homografts necessitates the use of xenografts in paediatric surgical reconstruction. Currently used xenografts are limited by immunoincompatibility and lack of growth, requiring repeat surgery to replace the defective graft. One approach to tackle acute immune rejection of animal tissue is aldehyde fixation of grafts prior to xenotransplantation. Though masking antigenicity, fixation is associated with multiple issues, including toxicity of fixative remnants and calcification. Decellularisation of biological grafts to remove foreign material is a promising alternative to reduce immunogenicity and dampen the risk of rejection. To combat lack of growth, in vitro cellularisation with stem cells is a promising solution. Purpose Current RVOT substitutes have not proven to be a lifelong solution for paediatric cardiac repair. This research has optimised a novel decellularisation technique for porcine RVOTs to produce a durable and cell-free scaffold with translational capability. Current work is looking to seed mesenchymal stem cells (MSCs) onto the acellular RVOT in vitro to enhance immune tolerance and endow the graft with growth capacity upon implant. Methods First, this decellularisation technique produces acellular RVOT scaffolds by simultaneously utilising three methods: enzymatic, chemical, and mechanical decellularisation. Novel to this method, the latter is realised using a 3D printed flow chamber to utilise the shear forces of continuous fluid flow for cell removal from the external and internal RVOTs structure. Coating the acellular graft with poly-lysine has then enabled recellularisation with MSCs isolated from the umbilical cord. Results Nuclei quantification demonstrates decellularisation of all structures of the RVOT, and elimination of residual nucleic acids is evidenced. Immunohistochemical analysis confirms the xenoantigen Galα1-3Galβ1-4GlcNAc-R is absent from the decellularised tissue. In parallel with removal of typical immunogens associated with the rejection response, the elastin and collagen fibres of the extracellular matrix are maintained, supported by histology and electron microscopy. Importantly, the tensile properties of the decellularised grafts are preserved. Finally, MSCs adhered onto the external pulmonary artery surface in vitro, achieving a uniform monolayer of cells. Conclusion RVOTs have been successfully decellularised and proven to possess seeding potential. Future work aims to characterise the seeded MSCs to assess parameters including proliferative capacity and expression of typical MSC markers. Overall, this work has the potential to produce a non-immunogenic graft possessing growth potential as a surgical alternative for paediatric RVOT replacement.

Root Characteristics and Its Correlation with Yield of Some Maize (Zea mays L.) Genotypes in Bangladesh

The growth capacity and yield characteristics of crop plants are significantly influenced by their roots. To determine the influence of root characteristics and its correlation with yield of maize genotypes, an experiment was conducted at the experimental field of Department of Crop Botany and Tea Production Technology, Sylhet Agricultural University (SAU), Bangladesh during December 2014 to May 2015. The experimental materials were ZM 0001, ZM 0002, ZM 0003, ZM 0004, ZM 0005, ZM 0006, ZM 0007 and BARI Maize 6. Three replications and a Randomized Complete Block Design (RCBD) were used to set up the experiment. Necessary cultural practices were followed as and when require during the growing period. Data were recorded on number of roots plant-1, root length, root spreading until grain filling stage (90 days after germination) and dry matter accumulation of roots at 30 days interval until harvest. The results indicated that root number of maize genotypes increased from 30 to 90 DAG at a constant rate and in the grain filling stage and it was limited within 32.33 to 42.67. Root length was also increased from 30 to 90 DAG at a constant rate and in the grain filling stage it was limited within 25.33 to 31.1 cm. Root spreading was continued to increase from germination stage to 90 DAG and it was varied from 22 to 27.73 cm. Dry matter accumulation of roots increased gradually from 30 to 60 and 90 DAG up to harvest. In this study, root shoot ratio (RSR) and root weight ratio (RWR) had significant correlation with maize yield. From the root length and root spreading data, it is suggested that moderate tillage and a plant to plant distance spacing of 25-30 cm can be maintained for better yield of maize.

The synergy of renewable energy consumption, green technology, and environmental quality: Designing sustainable development goals policies

AbstractGreen technology (GT) and the utilization of renewable energy (RE) are widely acknowledged as a catalyst for energy efficiency, economic growth, and a tool for combating environmental degradation. Although various studies have examined the ecological repercussions of these two improvements, they have primarily used traditional pollution indicators (namely, carbon emissions and ecological footprint) and ignored nations with the highest cleaner energy adoption. To bridge this gap, the present research seeks to analyze the relationship between GT, RE consumption, economic growth, trade, and load capacity factor in the top‐10 renewable economies. In addition to environmental pollution indicators, the study utilizes the load capacity factor as a novel proxy for environmental quality and investigates the load capacity curve hypothesis using the Cross‐sectional‐autoregressive distributed lag model from 1990 to 2021. According to the empirical findings, all explanatory variables have a significant long‐term effect on the load capacity. More precisely, GT and RE consumption contribute to the sustainability of the ecosystem, while trade increases the ecological deficit. Furthermore, the findings do not support the validity of the load capacity curve hypothesis, implying that the environmental restorative benefits of economic well‐being may not manifest in the latter stages of economic growth. Given these findings, policymakers in the top‐10 renewable economies should seize the environmental prospects offered by GT and RE and update trade and growth policies in a way that promotes biocapacity while simultaneously reducing the ecological footprint to reach the long‐term sustainable development goals (SDGs), particularly SDGs 7 and 13.

Soil–plant Integrative Supplementation with Humic Acid and Antioxidants Improves Growth, Fruit Quality, and Antioxidant Capacity of Cd-stressed Solanum Melongena

Soil–plant Integrative Supplementation with Humic Acid and Antioxidants Improves Growth, Fruit Quality, and Antioxidant Capacity of Cd-stressed Solanum Melongena

Radish and Spinach Seedling Production and Early Growth in Response to Struvite Use as a Phosphorus Source.

To sustain the increasing needs of a rapidly growing population, agriculture has relied on the use of synthetic fertilizers to intensify its production. However, the economical, environmental and health impacts associated with their use have raised significant concerns, especially given the scarcity of phosphorus. Utilizing nutrient-recovered materials like struvite can enhance circularity in agriculture and reduce its reliance on synthetic fertilizers. The objective of this study was to assess the implementation of struvite as a complete substitute to triple superphosphate, for radish and spinach seedling production and early growth, with or without supplementary fertigation. In addition, two rates of struvite were examined (0.68 and 1.37 g L-1 substrate) to evaluate its solubility. In the germination of radish, struvite had similar performance with conventional fertilization, while in spinach, the use of struvite decreased mean germination times. Both plants maintained comparable growth, chlorophyll content and antioxidant capacity when struvite was used, in comparison to conventional fertilizers. However, higher struvite rates under un-fertigated conditions significantly increased the chlorophyll b and total chlorophylls in the spinach, while phenolics and flavonoids decreased, contingent on the fertigation applications. In the radish, struvite maintained similar MDA and H2O2 levels to conventional fertilization, while decreases occurred in the spinach, with the application of ST1 under un-fertigated conditions, compared with conventional fertilization. The P and N contents of the plants were also affected, though these effects varied depending on the plant species, fertigation applications and struvite rates. This variance can be attributed to the characteristics of struvite, the plant species and the cultivation practices. The results of this study suggest that struvite can be successfully implemented in seedling production, establishing significant prospects for its commercialization and use in nurseries.

Eucommia ulmoides Oliv. leaf extract: Effects on growth, antioxidant capacity, and lipid metabolism in Red swamp crayfish (Procambarus clarkii)

The addition of Eucommia ulmoides Oliv. leaf extract (ELE) enhances growth, immunity and antioxidant capacity in crustaceans, which has not been studied on the red swamp crayfish (Procambarus clarkii). Hence, this study aimed to investigate the effect of ELE on the growth, antioxidant capacity, digestive enzymes activities, and lipid metabolism of red swamp crayfish. A total of 240 individuals of red swamp crayfish were randomly assigned to four groups. Each group was fed with feed a diet supplemented with varying doses of ELE: 0 g/kg (CK), 0.5 g/kg (T2), 1.0 g/kg (T3), and 1.5 g/kg (T4). After 30 days, the results indicated that T2 group exhibited a significantly higher final average weight, weight gain rate, and specific growth rate compared to the CK (P<0.05). Significantly lower feed conversion ratios than the CK (P<0.05) were observed in the T2 and T3 groups. For antioxidant indicators, hepatopancreas superoxide dismutase (SOD) activity was observed to significantly increased in the T3 and T4 groups, while levels of malondialdehyde (MDA) in which were significantly reduced in hepatopancreas and hemolymph (P<0.05). Regarding biochemical indicators and intestinal function, significant enhancement of lipase (LPS) activity was observed in the T2 group, which decreased in the T4, compared to the CK (P<0.05). Moreover, significant decrease of the glucose (GLU), total triglyceride (TG), and total cholesterol (TC) content of the hemolymph (P<0.05) were observed in the T4 group. Transcriptome analysis revealed that differentially expressed genes in the T4 group were primarily enriched in peroxisome proliferator-activated receptor (PPAR), and lipid metabolism pathways. Furthermore, it seems that ELE supplements significantly upregulated the expression of apoa-II, acaox1, acox3, me1, and plin1 in the PPAR signaling pathway to regulate lipid metabolism. In conclusion, 0.5 g/kg and 1.5 g/kg ELL addition amount can enhance growth performance, antioxidant capacity, and lipid metabolism of red swamp crayfish.

Transforming landscapes: Decoding the impact of universities on urbanization using advanced modeling and perception analysis.

Universities play a crucial role in urban economic and structural development. The government of Bangladesh has undertaken the initiative to establish a public university in each of the 64 districts. These newly founded universities have the potential to impact urban growth significantly. We aimed to project university-induced urban expansion to address this knowledge gap and identify the mechanisms driving urban growth. The classification of supervised and unsupervised methods was employed to analyze urban development for the years 2000, 2010, 2016, and 2022. We used the Cellular Automata and Markov Chain approach to forecast future urban growth and land transition capacity. Additionally, the driving factors and selection of the study area were derived from Focus Group Discussions (FGD), Key Informant Interviews (KII), Probit Model, and Perception Index (PI). The findings of this study reveal a 1.6% urban growth rate within ten years of the establishment of the university, while urban expansion accelerated to 29.78% after ten years. The predictions also indicate a sustained urban growth rate of 4.7% by 2042. Furthermore, the PI index demonstrates that the establishment of the university has resulted in high demand for rental housing, serving as one of the primary drivers of urban expansion. Moreover, the Probit model highlights strong economic capability, proximity to the town, railway station, hospital, and easy access to credit as vital facilitators behind the drivers of urban expansion. Policymakers, the scientific community, and urban planners can benefit from this study in pursuing sustainable city development through university establishment.

Evaluation of an engineered vascular graft exhibiting somatic growth in lambs to model repair of absent pulmonary artery branch

BackgroundGrowth is the holy grail of tissue implants in pediatrics. No vascular graft currently in use for surgical repairs of congenital heart defects has somatic growth capacity.MethodsBiologically-engineered grafts (6 mm) grown from donor ovine fibroblasts in a sacrificial fibrin gel were implanted into the left pulmonary branch of 3-month old lambs for 3, 6, and 18 months. A control group of Propaten® PTFE grafts was implanted for 6 months.ResultsThe engineered grafts exhibit extensive site-appropriate recellularization after only 3 months and near-normal increase of diameter from the preimplant value of 6 mm to 12.9 mm and also a doubling of length from 6.0 mm to 13.0 mm at 6 months (n = 3) associated with apparent somatic graft growth (collagen content increase of 265% over 18-month, n = 2), along with excellent hemodynamics and no calcification, in contrast to the Propaten® grafts. The left-right flow distribution is nearly 50–50 for the engineered grafts at 6 months (n = 3) compared to about 20–80 for the Propaten® grafts (n = 3), which have less than one-half the diameter, a 6-fold higher pressure gradient, and stunted vascular development downstream of the graft. The engineered grafts exhibit a stable diameter over months 12–18 when the lambs become adult sheep (n = 2).ConclusionsThis study supports the use of these regenerative grafts with somatic growth capacity for clinical trial in patients born with a unilateral absent pulmonary artery branch, and it shows their potential for improving development of the downstream pulmonary vasculature.

Exogenous Melatonin Reinforces Photosynthesis, Antioxidant Defense and Gene Expression to Ameliorate Na2CO3 Stress in Maize.

Salt stress can seriously affect the growth and development of maize (Zea mays L.), resulting in a great yield loss. Melatonin (MT), an indole hormone, is a potential enhancer of plant tolerance against salt stress. However, the complex mechanisms of MT application in enhancing maize salt tolerance are still unclear. Herein, three-leaf seedlings of salt-susceptible P138 and its salt-resistant ethyl methane sulfonate (EMS)-104 mutant were cultured with or without 150 μM MT application under 0 and 100 mM Na2CO3 treatments for seven days, to systematically explore the response mechanisms of exogenous MT in improving the salt tolerance of maize. The results showed that salt stress triggered an escalation in reactive oxygen species production, enhanced multiple antioxidant enzymes' activities, impaired cellular membrane permeability, inhibited photosynthetic pigment accumulation, and ultimately undermined the vigor and photosynthetic prowess of the seedlings. While suitable MT application counteracted the detrimental impacts of Na2CO3 on seedlings' growth and photosynthetic capacity, the seedling length and net photosynthetic rate of P138 and EMS-104 were increased by 5.5% and 18.7%, and 12.7% and 54.5%, respectively. Quantitative real-time PCR (qRT-PCR) analysis further showed that MT application activated the expression levels of antioxidant enzyme-related genes (Zm00001d025106, Zm00001d031908, Zm00001d027511, and Zm00001d040364) and pigment biosynthesis-related genes (Zm00001d011819 and Zm00001d017766) in both maize seedlings under Na2CO3 stress; they then formed a complex interaction network of gene expression, multiple physiological metabolisms, and phenotype changes to influence the salt tolerance of maize seedlings under MT or Na2CO3 stress. To sum up, these observations underscore that 150 μM MT can alleviate salt injury of maize seedlings, which may provide new insights for further investigating MT regulation mechanisms to enhance maize seedlings' salt resistance.

Assessing the Responsiveness of Moulay Ismail University to Teachers' Continuous Professional Development Needs: A Mixed-Methods Analysis

This study investigates the responsiveness of Moulay Ismail University (UMI) to the continuous professional development (CPD) needs of its teaching staff. The study integrates both quantitative and qualitative methods to gain a comprehensive understanding of teachers' perspectives. The quantitative phase involved administering a structured questionnaire to 214 teachers, focusing on their perceptions of the university's CPD offerings, including availability, relevance, accessibility, and institutional support. Descriptive statistics were used to analyze this data, revealing a predominantly negative attitude among teachers towards their university's ability to meet their CPD needs. The qualitative phase involved semi-structured interviews with 21 teachers. These interviews provided deeper insights into teachers' barriers, such as the lack of relevant CPD activities, the irrelevance between CPD content and pedagogical needs, and the overwhelming workload that limits their capacity for professional growth. Thematic analysis of the interview transcripts identified key themes that aligned with the quantitative findings, offering a more nuanced perspective on the challenges associated with CPD at the UMI. The study concludes that the teachers at the UMI perceive the institution as largely unresponsive to their CPD needs. This sentiment echoes broader concerns within the literature about the neglect of university teachers' professional development in Morocco. The findings underscore the need for more relevant and accessible CPD programs tailored to the specific needs of faculty and institutional reforms that alleviate the workload pressures that hinder professional growth.

Disruption of the signal recognition particle pathway leading to impaired growth, sugar metabolism and acid resistance of Lactococcus lactis

Lactococcus lactis is a well-known workhorse for dairy products, whose important industrial traits are tightly associated with numerous cytoplasmic membrane proteins. However, roles of the signal recognition particle (SRP) pathway responsible for membrane protein targeting have not been studied in L. lactis. In this work, the putative genes ffh and ftsY encoding SRP pathway components were identified in the genome of L. lactis NZ9000. Experimental evidence showed that sequence mutation in either the ffh or ftsY was not lethal, but prolonged the lag phase of the resultant mutants Δffh and ΔftsY by 2 h and lowered their biomass to 85.7 % of the wild type under static conditions, as well as deprived the mutants of improved growth capacity under aerobic respiration conditions. Besides, the speeds of glucose consumption and lactate production were significantly decreased in the mutants. Then, the impact of the SPR components on acid resistance was detected, showing that the ffh and ftsY were transcriptionally upregulated by 3.02 ± 1.21 and 8.66 ± 1.01-fold in the wild type during acid challenge at pH 3.0, and cell survival of the Δffh and ΔftsY decreased by10- and 100-fold compared with the wild type. To explore the possible mechanism about the SRP pathway involved in the above physiological traits, proteomics analysis was performed and revealed that disruption of the Ffh or FtsY led to decrease in ribosomal proteins, but increase in DnaK, GroEL and heat shock protein GrpE, indicating that the SRP pathway was closely linked to protein synthesis and folding in L. lactis. Decrease in the fructose-bisphosphate aldolase, respiratory complexes NADH dehydrogenase, as well as glutamate decarboxylase was also detected in the Δffh and ΔftsY, which is consistent with the phenomena of impaired sugar metabolism and acid resistance. Our results demonstrated the dispensable SRP pathway could contribute to the maintenance of metabolism homeostasis and acid resistance of L. lactis.

Effects of Light Spectra on Growth, Physiological Responses, and Antioxidant Capacity in Five Radish Varieties in an Indoor Vertical Farming System

Effects of Light Spectra on Growth, Physiological Responses, and Antioxidant Capacity in Five Radish Varieties in an Indoor Vertical Farming System

Linking intraspecies variability of Salmonella enterica isolates under acidic conditions to genotype.

There is a lack of information about Salmonella enterica strains under acidic conditions and their association with their genome. This study characterized intraspecies variability in the growth of 167 S. enterica isolates under two acid conditions (pH 4 and 5) and linked to the whole genome sequencing (WGS) data. A total of 1002 curves for each condition were obtained using turbidimetry measurements, and Baranyi and Roberts model was used to estimate the maximum rate of change (rcmax; OD600 nm h-1). Strains were categorized into slow, intermediate, and fast; and associations with their WGS data were performed. Huge variability in was observed at both conditions (pH 5=0.016-0.066 OD600nm h-1 and pH 4=0.003-0.028 OD600nm h-1). The majority of isolates was classified as intermediate (59.5% at pH 5 and 46.1% at pH 4). Strains classified as fast had a low frequency of allABCD genes at both pHs, and any of them having the presence of pefABCD, spvBCR, aadA2, dfrA12, and gyrA_D87G genes were linked to virulence or antimicrobial resistance. This study suggests that strains with fast capacity for growth under acidic conditions could have a fitness cost in their virulence or resistance potential. PRACTICAL APPLICATION: Data presented in this study could be used to select representative strains to evaluate the exposure assessment in different food items, mainly the growth and survival in acidic foods.

Alleviating heat stress on broiler breeder hens: effect of dietary antioxidant supplementation on reproductive performance, egg quality, offspring growth, and antioxidant capacity

Alleviating heat stress on broiler breeder hens: effect of dietary antioxidant supplementation on reproductive performance, egg quality, offspring growth, and antioxidant capacity

A Comparison of Standard SEI Growth Models in the Context of Battery Formation

Growth of the Solid Electrolyte Interphase (SEI) layer on negative electrode particles during the formation cycle is one of the most complex and least understood steps of lithium-ion battery manufacturing. This initial SEI formation significantly impacts battery performance, lifetime, and degradation. Zero-dimensional models, which reduce the complexity of SEI’s morphology, material, and structure, are commonly used to study long-term SEI growth rates and capacity fade. These models are derived based on limiting mechanisms. We aim to compare the most common SEI growth models, focusing on the first few cycles at low C-rates representing formation protocols. Using consistent parameters across models, we seek to understand if they can capture the dynamics of SEI formation. We conducted qualitative comparisons with experimental measurements of Coulombic efficiency in 2032-type coin cells at low C-rate. Our analysis shows that the models predict SEI growth in the first cycle to be higher than in subsequent cycles. However, the difference between cycles in these models is insufficient to explain the experimental results, which indicate a capacity fade during the first cycle that is two orders of magnitude higher than in later cycles. This suggests new models are needed to accurately describe the physics of the formation cycle.

Research on the Evaluation of Competitiveness of Steel Industry Enterprises

The steel industry, as a fundamental industry in the organic development framework of China's economy, has been facing the long-standing problems of production exceeding consumption, high asset liability ratio, severe product homogenization competition, and high pollution and carbon emissions in the production process in recent times. At the same time, due to the impact of the sluggish demand caused by the COVID-19, the rapid rise in the cost of raw materials such as iron ore, and the gradual disappearance of a series of policy dividends such as supply side reform, the financial situation of steel enterprises is becoming more and more pessimistic. How to cope with the increasing financial risks and gain competitive advantages through strengthening financial competitiveness in the fierce market competition has become a major issue that China's steel enterprises urgently need to solve. This article selects steel industry enterprises as the research object for analysis. By reviewing relevant literature on enterprise competitiveness, the relevant concepts of enterprise competitiveness are extracted, and the research results and evaluation system are organized. Based on factor analysis, 16 targeted indicators are selected based on scale, efficiency, growth, operational capacity, debt paying capacity, and research and development capacity. An analysis model is constructed and analysis conclusions are drawn. In the analysis results, problems are identified regarding the poor competitiveness of steel industry enterprises. The following research results were obtained: 1) The overall competitiveness score of enterprises in the industry is not high, all less than 1. Among them, operational capability has the greatest impact on the competitiveness of steel industry enterprises, followed by scale, debt paying ability, efficiency, research and development ability, and growth. 2) The worst scoring indicators among the six enterprise competitiveness indicators are the scale indicator and the growth indicator. There is a significant gap in industry scale, with 12 companies scoring positive in the scale factor, accounting for 34.29% of the industry; The scale factor score of 23 enterprises is negative, with an industry proportion of 65.71%. In addition, there are growth indicators, with 12 companies in the industry having positive growth factor scores and 23 companies having negative growth factor scores, indicating a significant head effect on overall growth capacity.

SNP Markers: Analysis of Genetic Diversity and Identification of Genomic Regions in Pantaneiro Sheep and Texel Sheep Under Natural Selection.

Sheep farming is growing substantially in Brazil, driven by the increasing demand for sheep meat. This rising demand has heightened the focus on sheep, making them the subject of numerous studies, including those centered on genetic analysis. A notable research area involves Pantaneiro sheep, which are indigenous to the Pantanal region of Mato Grosso do Sul and other locations. These sheep are of particular interest due to their adaptation to the unique environmental conditions of the Pantanal, a floodplain characterized by its distinctive climatic and ecological features. This study primarily aimed to conduct a comprehensive genomic analysis of Pantanal sheep subjected to natural selection within the Pantanal region and compare different sample herds using methodological approaches. Genomic analysis was performed to examine genetic diversity and structure via GGP50K single nucleotide polymorphism (SNP) analysis. A sample of 192 adult sheep over 4 years old was categorized into seven populations based on location: Six populations comprised Pantaneiro sheep with one Texel sheep population. Outlier SNPs were assessed to pinpoint regions under natural selection, with comparisons between the Pantaneiro and the commercial Texel breeds. All data analyses were conducted using the R programming language, employing specialized genetic analysis packages. These outlier SNPs were detected using three methodologies, PCAdapt, OutFLANK, and FDIST2/fsthet, with false discovery rate (FDR) corrections applied to ensure result accuracy. Each method was evaluated, and the genes associated with the identified SNPs were cross-referenced with the most recent sheep genome database, focusing specifically on genes with known phenotypic traits. Analysis of a sample comprising 192 adult individuals revealed greater genetic variability within the Pantaneiro breed than the Texel breed, highlighting the adaptation of the Pantaneiro breed to the unique Pantanal environment. Conversely, the Texel breed exhibited significantly higher levels of inbreeding, attributed to its controlled breeding practices. Outlier SNPs were detected with notable variation across different methodologies, underscoring the importance of FDR correction in ensuring the reliability and concentration of identified outliers. These outlier SNPs facilitated the identification of genes associated with key phenotypic traits, including hair growth, tissue regeneration, pigmentation regulation, and muscle capacity. The integrated analysis of methodologies demonstrated significant efficiency in elucidating the genomic landscape of Pantanal sheep, highlighting the genetic richness inherent in sheep from the Pantanal region of Mato Grosso do Sul. The techniques employed effectively identified outlier SNPs associated with phenotypically relevant genes. These findings, which reveal greater genetic variability and adaptability, underscore the potential of these animals for future research and their significance within Brazilian sheep farming. The Texel breed served as a valuable comparative group, illustrating the limited genetic variability in highly controlled breeding environments.

Protective Role of Sulforaphane against Physiological Toxicity of Triphenyltin in Common Carp (Cyprinus carpio haematopterus).

This experiment mainly explored the protective role of sulforaphane (SFN) against physiological toxicity of triphenyltin (TPT) in Cyprinus carpio haematopterus. In total, 320 Fish (56.90 ± 0.40 g) were randomly divided into four groups with four replicates each. The control group was fed the basal diet, the TPT group (TPT) was exposed to 10 ng L-1 TPT on the basis of the control group, the SFN + TPT group (TPT + SFN) was fed a diet supplemented with 10 mg kg-1 SFN on the TPT group, and the SFN group (SFN) was fed a diet supplemented with 10 mg kg-1 SFN. After 56 days of breeding trials, the results showed that TPT exposure resulted in a remarkable decrease (p < 0.05) in final weight, weight gain rate (WGR), specific growth rate (SGR), and condition factor (CF), but an increase (p < 0.05) in feed conversion ratio (FCR) and hepatosomatic index (HSI) of fish. TPT treatment decreased (p < 0.05) the amounts of hematocrit (Hct) and hemoglobin (Hb), plasma complement component 3 (C3) and C4 contents, alternative complement pathway (ACH50), acid phosphatase (ACP) and lysozyme (LZM) activities, liver glutathione (GSH) content, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX) activities, interleukin 10 (IL-10), and SOD mRNA expressions, but increased (p < 0.05) plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, liver malonaldehyde (MDA) content, tumor Cyclooxygenase 2 (COX2), and necrosis factor α (TNFα), IL-1β, and MDA mRNA expressions. A histological analysis of the liver showed that a higher occurrence rates of the hepatocyte hypertrophy, nuclear disappearance and hepatocyte vacuolization was observed in the hepatocytes of fish exposed to TPT, and it was accompanied by the dilation of hepatic sinusoids. In addition, the toxicity induced by TPT was significantly improved in the groups that were treated with SFN, and SFN was able to improve growth performance and immunity, alleviate TPT-induced changes in inflammatory factors, ameliorate oxidative stress, and increase the activity of antioxidant enzymes (p < 0.05). The addition of SFN also alleviated liver damage caused by TPT and protected the structural integrity of the liver. Overall, these findings suggest that TPT inhibited the growth, immunity, and antioxidant capacity of Cyprinus carpio haematopteru. Dietary SFN could be beneficial for growth promotion, immunity, antioxidant capacity, and protection of liver structural integrity. Therefore, SFN is a prospective feed supplement for ameliorating the damage caused to fish by TPT contamination.