Group T1 Research Articles

The incidence of adding Gingko biloba and Curcumin extracts in drinking water on Body growth, hematological and immune statues of New Zealand White rabbit

This study was performed to investigate the effect of adding ginkgo biloba (GB) and curcumin extracts in rabbit drinking water, as growth promoter, serum biochemistry, antioxidant enzyme activities and immunoglobin statues. A total of 40 New Zealand White rabbit (NZW) at 5 weeks of age assigned to 4 treatments with four replicates. Gingko biloba (T2) and Curcumin extracts (T3) and their mix (T4) had no significantly differences among all treatments in initial weight, feed and water intake, also in body temperature. While, body weight and body weight gain had significantly (P≤0.05) increased in the all groups of water additive compared with control (T1). As well as FCR (feed conversion ratio) had significantly (P≤0.05) improved in the treatments T2, T3 and T4, compared with the control group T1. Otherwise, the heart rate (beat) was significantly (P≤0.01) decreased in the treatments respectively compared with the control T1. Quite good results were achieved that approve the curcumin and ginkgo biloba extract has a direct influence on the production and blood parameter, improve immunoglobins and antioxidant enzymes concentrations in blood serum.
 

Impact of Spraying and Dipping Organic Acids on Hatching traits, Chick Quality, Immunity and eggshell bacterial count

Organic Acids (BacterActive) spraying and dipping on hatching eggs were evaluated experimentally for their effect on embryonic mortality, hatchability, chick quality, immunity, and eggshell bacterial count. A total of 6300 hatching eggs of broiler breeder (Ross-308) at age 58 weeks, were collected and divided to 7 treatments as follows: (T1: General Control, T2: Dipping Water, T3: Spray Water, T4: Dipping and spraying water, T5: Dipping organic acid, T6:spray Organic Acid, T7:dipping-spray organic acid ) Results indicated that using Organic acid (BacterActive) a synergetic blend of formic – propionic - lactic acids applied in a spraying manner with a concentration of 1ml/l significantly (p≤0.01) increased hatching traits, chick quality, and chicks’ antibody titer against Newcastle Disease (ND) and Infectious Bronchitis (IB) also organic acid application significantly reduced embryonic mortality and eggshell total bacterial count in all treatments. while T6 showed the best result among all treatments that used organic acid and T5 showed the lowest value. among control group T3 had the best overall result while T1 shows least favorable result of all treatments. A special note to take in consideration is that dipping eggs in water T2 had least impact on embryonic death. In conclusion results showed that organic acids application via spraying, dipping, and dipping-spraying in broiler breeders leads to significant improvement of hatchability, chick quality and immune status while decreasing embryonic mortality and eggshell total bacterial count.

Effectiveness of combined seeds (pumpkin, sunflower, sesame, flaxseed): As adjacent therapy to treat polycystic ovary syndrome in females.

The formation and release of eggs during ovulation are impacted by high amounts of androgens. Seed cycling is powerful in the treatment of polycystic ovary syndrome (PCOS). For efficacy studies, 90 women with PCOS, between 15 and 40 years were selected from the department of gynecology, Tertiary care unit. Women with PCOS were divided into three groups (T0, T1, T2) (20 women/group). Among these three groups, the first was the control group (T0). The second group was the experimental group (T1). In T1, 20 women with PCOS were treated with a portion control diet and METFORMIN 500 mg tab/day for 90 days. The third group was also an experimental group (T2). In this group, 20 women with PCOS were also treated with another treatment plan for 90 days, in which portion control diet and seed cycling were included. During the 12-week efficacy trial, the highest follicle stimulating hormone (FSH) levels were found in the control group T0 (8.18 ± 0.13 mIU/mL). In T2, FSH falls from 6.545 ± 0.16 mIU/mL to 3.51 ± 0.13 mIU/mL throughout a 12-week period. Overall, a portion-controlled diet and seed cycling reduced FSH levels by 1.2% to 2.5%. LH value was 10.118 ± 0.1874 IU/L in the control group (T0), which climbed 12.82 ± 0.15 IU/L, but decreased from 10.62 ± 0.26 IU/L to 9.79 ± 0.17 U/L and 11.015 ± 0.24 IU/L 6.217 ± 0.167 IU/L in the other groups (T1, T2). It was determined that the LH levels were reduced by 1.5%-2% in T2. Conclusively, the seed cycling approach is effective and has significant results in women with PCOS. Seed cycling improves hormonal disturbance in women which promotes a healthy life.

Analysis of the Potency of Robusta Coffee (Coffea canephora) to Increase the Expression of FGF2, Collagen 1 and ALP in the Periodontal Ligament During Orthodontic Tooth Movement

Orthodontic treatment is relatively long and various efforts have been made to speed up the movement of orthodontic teeth. Coffee contains substances called caffeine (1,3,7 trimethylxanthine), chlorogenic acid and caffeic acid, as well as flavonoids which have antioxidant effects. Chlorogenic acid and caffeic acid are antioxidants that can reduce oxidative stress in osteoblasts and promote osteogenesis. The study aimed to analyze the expression of FGF2, Collagen 1 and ALP, on the movement of orthodontic teeth after administration of Robusta coffee extract. 24 wistar rats divided into 4 groups, namely groups C1 and C2: Rats were given orthodontic mechanical force (OMF) for 2 and 3 weeks and groups T1 and T2: Mice were given OMF and Robusta coffee extract for 2 and 3 weeks. OMF in mice (rats) was carried out by means of the right maxillary first molars teeth and on both maxillary incisors were given ligature wires. Then the right maxillary first molars moved to the mesial with a Niti closed coil spring. Observations were made on the day 15 and day 22 with immunohistochemical examinations to determine the expression of FGF2, Collagen 1 and ALP. The results indicated that administration of Robusta coffee extract for 2 and 3 weeks significantly increased the [expression of FGF2, Collagen 1 and ALP. The expression of FGF2, Collagen 1 and ALP (expression of GFß1, IGF1 and PDGF). The expression of TGFß1, IGF1 and PDGF during 3 weeks was greater than that at 2 weeks (p < 0.05. Robusta coffee extract can be used as an alternative ingredient in accelerating orthodontic treatment. HIGHLIGHTS Orthodontic treatment requires quite a long time and various efforts have been made to speed uporthodontic tooth movement, for example with drugs Robusta coffee extract contains ingredients that have antioxidant effects that can reduce oxidativestress on osteoblasts and promote osteogenesis Robusta coffee extract effectively increases the expression of FGF2, Collagen-1 and ALP which are bone formation markers GRAPHICAL ABSTRACT

Encapsulated pepper blend in the diet of confined Holstein bullocks: effect on ruminal volatile fatty acid profiles, growth performance, and animal health

Dairy bulls in feedlots have been a viable alternative for dairy producers to reinforce the family's income. Aspects such as balanced diets and proper management are essential for these animals to develop and allow an economic return fully. Plant extracts are performance enhancers and ruminal and intestinal health promoters. Therefore, this study aims to evaluate whether the addition of encapsulated pepper (EP) blend (Capsicum annuum, Capsicum frutescens, and Capsicum chinense - rich in capsaicin) interferes with the volatile fatty acid profile in the rumen and enhances the growth performance of Holstein bullocks in a feedlot. For the experiment, 24 whole bullocks were used, distributed into three treatments, with eight replicates per treatment (one animal as an experimental unit, kept in an individual stall): groups T0, T200, and T400, receiving 0 mg, 200 mg, and 400 mg EP/kg of concentrate, respectively. Knowing the intake of concentrate and the average body weight during the experiment, we calculated the dose in mg/kg/day of the EP; that is, the T200 animals consumed 2.45 mg EP/kg (body weight -BW)/day; and T400 consumed 4.9 mg EP/kg BW/day. The animals from T400 presented a more significant weight gain between days 15 and 45 of confinement compared to T0 (P=0.05). This same treatment (T400) had a trend of lower weight gain between days 46 and 90 (P=0.09). Likewise, the T400 group had higher feed efficiency than T0 between days 15 and 45. Furthermore, the treatments affected the white blood cell count, with the T400 bullocks showing a higher number of neutrophils and lymphocytes. Higher levels of C-reactive protein (CRP) were measured in the serum of steers from both groups that consumed pepper (P<0.01). Interaction between treatment × day was observed for the activity of glutathione enzymes (GST and GPx) and levels of lipoperoxidation (LPO) (characterized by antioxidant stimulation) associated with the reduction in serum LPO; similar antioxidant enzymes behavior was observed in the liver. In the small intestine (jejunum), the activities of antioxidant enzymes (GST and GPx) were lower in the two groups of cattle that consumed EP, and LPO was lower. The treatments affected the concentration of acetic acid in the rumen fluid, presenting lower levels in T400 compared to T200 and similar T0 (P≤0.05). There was an interaction of day vs. treatment for propionic acid, presenting a higher concentration on day 45 at T400 than T0. These results, therefore, allow us to conclude that adding 400 mg of pepper extract can be an excellent additive for weight gain at the beginning of the experiment; however, over time, this dose of additive negatively affects weight gain. Both EP doses stimulated serum and tissue antioxidant responses, reducing lipoperoxidation. However, the 400 mg EP/kg concentrate suggests a pro-inflammatory response (leukocytosis and elevated CRP), s probably related to the high dose (i.e., between 1.7 and 2.4 g/animal/day).

Association between endometrial blood and clinical outcome in frozen single blastocyst transfer cycles.

Background: The success of embryo transfer cycle depends mainly on the quality of embryo and endometrial receptivity. Ultrasound examination is still the most widely used non-invasive evaluation method for its advantages of convenience, non-invasiveness and repeatability. Ultrasound-measured endometrial blood flow is one of the important evaluation indicators of morphology. Aims: To investigate the effect of the number of endometrial blood flow branches on pregnancy outcome of frozen-thawed embryo transfer cycles which have undergoing hormone replacement therapy (HRT-FET). Material and methods: A retrospective cohort study was performed looking at a total of 1390 HRT-FET cycles from our reproductive medicine center between January 2017 to December 2021, which transferred one blastocyst frozen on day 5 with good quality in morphology. Associations between endometrial blood flow branches and pregnancy outcomes were evaluated with multivariable linear regression analysis. Results: The number of endometrial blood flow branches was independently associated with clinical pregnancy (OR 1.10; 95% CI 1.02-1.20). After adjusting for potential confounders, the effect size (odds ratio) was 1.09 (95% CI 1.00-1.19), and the results showed that the clinical pregnancy rate and live birth rate of T2 and T3 groups were significantly higher than those in group T1 (p < 0.05). Subgroup analysis showed that a consistent association between the endometrial blood flow branches and clinical pregnancy in all subgroups. Conclusion: Our study provided evidence for the influence of endometrial blood flow on pregnancy outcomes. There may be an independent association between the number of endometrial blood flow branches and pregnancy outcomes in frozen-thawed single blastocyst transfer cycles.

Bacterial Growth in Unsealed Bone-grafting Products

The aim of this study was to simulate reusing an opened package of bone substitute (BS) under typical clinical conditions, by analyzing bacterial growth in samples of resealed packages of alloplast and xenograft BS following different time periods up to a week. The experiment was performed in 2 stages: dry stage and wet stage. In the dry stage, BS samples were exposed to air for 15 minutes and then were sealed again in their original package. After several "aging" periods [no aging (t0), 24hours (t1), and 1week (t7)], BS samples were plated onto brain heart infusion agar and were incubated at 37°C for 24 hours under aerobic conditions. Bacterial colonies were enumerated and colony forming units per 1mL was calculated. In the wet stage, the experiment was repeated but BSs were left to age in a brain heart infusion broth to simulate a more supporting humid environment to encourage bacterial growth. In the dry stage of the experiment, no bacterial growth was detected in both xenograft and alloplast samples (colony forming units/mL=0), in any of the time points (t0, t1, t7). In the wet stage, average bacterial growth was 0 for both groups in t0. In t1: 1.14 × 10 4 ±4.16 × 10 3 for the xenograft and 7.73 × 10 5 ± 9.99 × 10 5 for the alloplast. In t7: 1.64 × 10 9 ±1.82 × 10 8 in the xenograft compared with 8.86 × 10 8 ±4.00 × 10 8 for the alloplast. Analysis of variance with repeated measures showed a significant difference in comparison of the 2 BSs for each time group (t1 and t7) ( P <0.005). Under dry conditions, in a resealed package, there was no bacterial growth for up to 1 week for both BSs. Once the BSs are introduced to a humid environment, which supports bacterial growth, the xenograft BS allowed more rapid bacterial growth, compared with the alloplastic BS.

Nutrient utilization and carcass traits in broiler chickens as affected by nano-chromium supplementation

The present study was carried out to perceive the effects of dietary supplementation of nano-chromium on nutrient utilization and carcass traits in broiler chickens. For this, 120 unsexed day-old broiler chickens were procured and randomly allocated into four different treatment groups with three replicates in each. The control group (T0) was fed with a standard basal diet as per BIS (2007). Treatment groups T1, T2, and T3 were fed the basal diet with 400, 800, and 1600 ppb levels of nano-chromium, respectively for six weeks. Dry matter, crude protein, and total carbohydrate metabolizability were significantly improved in treatment groups T2 and T3; while organic matter and ether extract retention were found significantly higher in treatment groups T1, T2, and T3 when compared with a control group. There was no significant difference in the yield of cut-up parts (% live body weight). The supplementation of nano-chromium did not bring any significant changes in processing loss i.e. blood, feather, head, and shank, but abdominal fat was significantly reduced with increasing levels of nano-chromium. Hence, it can be concluded that dietary supplementation of 1600 ppb nano-chromium in the diet of broiler chicken improved nutrient utilization and lowered abdominal fat in broiler chicken.

Effect of dietary inclusion of mealworm frass on growth, hematology, and serum biochemistry of sheep.

Frass is the main component of worm by-product which exhibit anti-microbial and anti-pathogenic properties. In the present study, we assessed the possibility of mealworm frass in sheep feeding regime and evaluated its effect on health and growth performance of sheep. A total of 09 experimental sheep (18-24months of age) were grouped into three categories (T1, T3, and T3); each group comprised 3 animals including 2 males and 1 female. Group T1 was considered control, group T2 contains 75% commercial feed and 25 mealworm frass, and T3 was 50:50 of commercial feed and mealworm frass. The sheep in group T2 showed average weight gain of 2.9kg; however, when the dietary inclusion increased up to 50% of mealworm frass or decreased up to 50% of concentrate feed, the average weight gain decreased up to 2.01kg (group T3). Moreover, the sheep fed with 25% mealworm frass exhibited the lowest feed refusal percentage (6.33%) in total duration of the dietary period (6weeks). The highest volume of RBC was found in blood collected from sheep fed within group T2 (10.22 1012/L ± 0.34), followed by sheep fed in group T3 (8.96 × 1012/L ± 0.99) (P < 0.05). Significantly (P < 0.05) highest MCV volume in fL (femtoliter) was found in group T2 (32.83 ± 0.44) followed by group T3 (31.23 ± 0.23). The animals in group T3 showed significantly (P < 0.05) highest MCHC volume (40.47g/dL ± 0.62) followed by group T2 (38.77 ± 0.97). Similar trend was found in MPV (fL); significantly (P < 0.05) highest MPV volume was found in group T3 (12.63 ± 0.09) followed by group T2 (12.53 ± 0.33). Significantly (P < 0.05) high serum phosphorous (P) (6.00 ± 0.29), TG (60.03 ± 3.11), and TP (7.63g/dL ± 0.23) levels were found in group T3, followed by animals in group T2. We can conclude that inclusion of mealworm frass to replace 25% commercial concentrate feed improved the growth rate and overall health status of the sheep. The present study laid a foundation for the utilization of the mealworm frass (waste product) in ruminant feeding.

Initial forces acting on the maxillary teeth with aligner activation of canine distalization on the basis of different initial canine tips in vitro

The objective of this study was to investigate the 3-dimensional forces exerted on the maxillary teeth with aligner activation of maxillary canine distalization on the basis of different initial canine tips invitro. A force/moment measurement system was used to measure the forces exerted by the corresponding aligners with the same activation of 0.25 mm for canine distalization on the basis of the 3 initial canine tips. The 3 groups included: (1) group T1, with canine inclined mesially 10° on the basis of the standard tip; (2) group T2, with canine maintained standard tip; and (3) Group T3, inclined distally 10° on the basis of the standard tip. A sample size of 12 aligners was tested for each of the 3 groups. The canines were subjected to distomedial forces and labiolingual and vertical components, which are minimal in group T3. As the anterior anchorage for canine distalization, the incisors were mainly subjected to labial and medial reaction forces, with the most significant reaction forces in group T3, and the lateral incisors were subjected to greater forces than the central incisors. The posterior teeth mainly received medial forces, which were greatest when the pretreatment canines were distally tipped. The forces on the second premolar are greater than those on the first molar and the molars. The results demonstrate that attention to the pretreatment canine tip is necessary when performing canine distalization with aligners, and further work, both invitro and clinically based, investigating the effect of the canine initial tip on maxillary teeth during canine distalization would greatly aid in better treatment protocol with aligners.

Effect of prebiotic alternative to antibiotics in ration on meat quality parameter and cooking properties of broiler meat

The research was conducted to investigate the effect of prebiotic on nutritional composition and cooking properties of broiler meat. 120 Habbard classic broiler male chicks were divided at random into five dietary treatment groups, each with three replications of eight chicks. The dietary treatment included T1= None of prebiotic or antibiotic; T2= 50mgantibiotic/kg of diet; T3=0.5g of prebiotic/kg of diet; T4= 1g of prebiotic/kg of diet and T5= 2g of prebiotic/kg of diet during the entire period. The commercial name of this prebiotic is AGREMOS. The T5 treatment group recorded the highest length and diameter of the drumstick (129.95mm and 63.49mm), while the T2 and T1 dietary groups recorded the lowest length and diameter of the drumstick (129.07mm and 40.81mm, respectively). Prebiotic significantly (p&lt;0.001) affects the diameter and length of various broiler body parts as well as their weight (aside from dressing and feathers), drip loss, cooking loss, and cooking yield. CP%, EE%, Ash% of drumsticks and DM%, moisture%, and CP% of breasts were significantly (p&lt;0.001) affected by prebiotic. For drumsticks, the dietary group T5 had the highest CP% and EE% at 27.86 and 2.63, respectively, while T1 and T3 had the lowest values at 24.01 and 2.47. In terms of breast muscle, T5 had the highest CP% (27.86) and T1 had the lowest CP% (24.01). Prebiotic is an excellent substitute for antibiotics in broiler diet. When compared to the other treatments, it was discovered that treatment T5 (2g prebiotic) was superior for the diet of broilers.

Effects of adding Moringa oleifera seeds on the performance and production of common carp (Cyprinus carpio L.)

The objective of this study was to assess the effects of utilizing Moringa oleifera at various inclusion levels in fish meal-based diets for common carp fish. In this study, we employed seeds at various concentrations, such as 2% Moringa oleifera seeds (MOS) for T2, 4% MOS for T3, and 6% MOS for T4, in comparison to control treatment T1 without it (0% MOS). The treatment T4 substantially (P&gt; 0.05) reached the highest specific growth rate, which was recorded at (0.781%), before dropping to records of (0.689%, 0.610%, and 0.229%) for treatments T3, T2, and the control group T1. Additionally, Treatment (T4) had the highest relative growth rate (13.25%), which then decreased to records of (11.21%, 9.567%, and 3.033%) for Treatments (T3), T2, and Control Treatment (T1), respectively. The treatment T4 reached the maximum value of the daily growth rate, which was recorded at (0.283%), before dropping to records of (0.238), (0.2203), and (0.063%) for the treatments T3, T2, and the control treatmentT1, respectively. Treatment (T4) had the highest reported value of the ultimate body weight, coming in at 49.44g. After then, it started to fall, and records of 45.49g for Treatments (T3), 42.43g for Treatments (T2), and 30.51g for Control Treatment were made (T1). The total recorded feed intake for treatment (T4) was 7.54g, which was the highest value. Following that, it began to decrease, and measurements of 7.07g for Treatments (T3), 6.77g for Treatments (T2), and 5.83g for Control Treatment were made (T1). The body gained weight to its highest ever recorded level of 92.74g as a result of treatment (T4). It then began to decline, with records of 78.43g for Treatments (T3), 66.96g for Treatments (T2), and 22.21g for Control Treatment (T1). Significantly, Treatment (T4) contributed to the feed conversion ratio increasing to 3.16%, the highest level ever recorded. After that, it started to go down, with records of 2.83% for Treatments (T3), 2.51% for Treatments (T2), and 0.92% for Control Treatment (T1). Contrarily, partly as a result of the Control Treatment, the Feed Efficiency Ratio improved to 1.09%, the highest level ever recorded (T1). After that, it began to decline, with records of 0.39 percent for Treatments (T2), 0.35 percent for Treatments (T3), and 0.32 percent for Treatment (T4). The effect of the treatment caused the Protein Efficiency Ratio to rise sharply to its highest recorded value of 0.81%. (T4). After then, it started to fall, with records for Treatments (T3) of 0.68 percent, Treatments (T2) of 0.59 percent, and Control Treatment of 0.19 percent (T1). The greatest level of Protein Productive Value was likewise attained by Treatment (T4), which was 3.65%; thereafter, Treatments (T3), T2, and Control Treatment (T1) each achieved records of 3.32%, 2.98%, and 3.33 %. The maximum Fat Efficiency Ratio was attained by Treatment (T3) (10.49%), but it thereafter dropped to records for Treatments (T4), (T2), and Control Treatment (T1) of 8.43%, 7.75%, and 1.70%, respectively. The aim of this study is for the purpose of providing healthy nutrition requirements for fish. It concludes that T4 treatment which contains 6% moringa seeds was the best ratio to obtain best growth performance for common carp.

Effect of Sundried Cassava Peel Meal-Based Diet Supplemented with Quadraxyme® on Performance and Blood Profile of Grower Boar Pigs

Introduction: Monogastric animals such as pigs and poultry cannot handle cellulose, hemicellulose, and lignin, which form the major components of agro-industrial products. This feeding trial was carried out to evaluate the responses of growing boar-fed sundried cassava peel meal (CPM)-based diet supplemented with the enzyme. Materials and methods: Thirty-two growing boars (Large white x Landrace), eleven weeks old with an average weight of 25.00 ± 0.30 kg, were allocated randomly to dietary treatments. A basal diet was compounded containing 48% sundried CPM. The dietary treatments were T1 (control) supplemented with 0.0g/100g, and other treatments, including T2, T3, and T4, received 0.5g/100kg, 1.5g/100g, and 2.0g/100g levels of Quadraxyme® supplementation, respectively. Quadraxyme® is a product of Quadragen Veterinary Health Private Limited. Quadraxyme® contains amylase, cellulase, xylanase, pectinase, lipase, arabinose, α-galactosidase, and β -glucosidase. Results: This study showed significant differences in average feed intakes and daily weight gains across the dietary treatments. Pigs in the T4 group had the highest weight gain, followed by those in T2 and T3 groups, while the minor weight gain was in the diet with no enzyme supplementation. The dietary treatments significantly influenced hematological parameters in almost all the studied indices. The mean corpuscular hemoglobin concentration (MCHC) values of pigs fed the T1 diet recorded significantly higher MCHC and a reduction in concentrations of packed cell volume (PCV), red blood cell (RBC) counts, and a slight increase in mean corpuscular volume (MCV) concentration. The dietary treatments did not affect the total blood protein and albumin levels of the growing pigs enrolled in this investigation. The result showed that the levels of enzyme supplementation to the SDCPM-based diet did not significantly influence the electrolytes of the growing boars. The PO42- increased in enzyme-supplemented diets, and group T4 supplemented with 0.20g/100kg of the enzyme recorded the highest PO42- value (6.79mg/100ml) among the other groups. Conclusion: It is concluded that including 0.20g/100kg in a 48% sundried CPM-based diet for growing pigs enhanced growth and feed conversion, improved phosphorus utilization, and had no adverse effects on growth and blood parameters.

Nasal immunization with AMH-INH-RFRP DNA vaccine for improving follicle development and fertility in buffaloes.

Inhibin DNA vaccine has already been proven to improve the fertility of animals. This study aimed to investigate the effects of a novel Anti-Müllerian hormone (AMH)-Inhibin (INH)-RF-amide-related peptides (RFRP) DNA vaccine on immune response and reproductive performance in buffalo. A total of 84 buffaloes were randomly divided into four groups and nasally immunized twice a day with 10 ml of either AMH-INH-RFRP DNA vaccines (3 × 1010 CFU/ml in group T1, 3 × 109 CFU/ml in group T2, and 3 × 108 CFU/ml in group T3) or PBS (as a control) for 3 days, respectively. All animals received a booster dose at an interval of 14 days. ELISA assay revealed that primary and booster immunization significantly increased the anti-AMH, anti-INH, and anti-RFRP antibody titers in the T2 group compared with that in the T3 group. After the primary immunization, the antibody positive rate was significantly higher in the T2 group than that in the T3 group. In addition, ELISA results indicated that concentrations of E2, IFN-γ, and IL-4 were significantly higher in the antibody-positive (P) group compared to the antibody-negative (N) group. In contrast, there was no significant difference in the concentrations of P4 between the P and N groups. Ultrasonography results revealed a highly significant increase of 2.02 mm in the diameter of ovulatory follicles in the P group compared to the N group. In parallel, growth speed of dominant follicles was significantly higher in the P group than that in the N group (1.33 ± 1.30 vs 1.13 ± 0.12). Furthermore, compared to N group, the rates of oestrus, ovulation, and conception were also significantly higher in the P group. The novel AMH-INH-RFRP DNA vaccine improves the proportion of oestrus, ovulation, and conception in buffalo by promoting the production of E2 and the growth of follicles.

Dietary utilization of mealworm frass in rabbit feeding regimes and its effect on growth, carcass characteristics, and meat quality.

This study aimed to shed light on the use of mealworm (Tenebrio molitor) frass (TMF) in rabbit diets and its effects on growth performance, blood profiles, rabbit meat quality, and fatty acid profiles. A total of 48 Gabali rabbits were divided equally and randomly into four groups to be fed one of four dietary treatments: a control (T0) group and three TMF groups, which included TMF meal at 1 (T1), 2 (T2), and 3 (T3) %, respectively. The rabbits were fed on isonitrogenous and isoenergetic diets for 11 weeks, and the growth performance data were recorded. Six rabbits per group were slaughtered at the end of the 11th week, then the pharmacochemical parameters and carcass traits were measured, and meat quality and fatty acid profiles were analyzed. The results indicated that the growth performance of different groups was similar (P > 0.05). The levels of globulin, glucose, and alanine transaminase (ALT) were lower in the mealworm frass groups compared with the control group. Carcass traits were not affected by experimental regimes. Fat was higher in the TMF treatment groups, while moisture was lower compared with the control group. The shear force had a lower value in the treatment groups T3 and T2 compared with the control group. The highest values of redness and chroma (color parameter) of rabbit meat were observed in the treatment groups vs. the control group. Moreover, the proportion of total saturated fatty acids in the meat of rabbits that were fed on the T2 and T3 diets was higher compared to those fed on the control (i.e., T0) and T1 diets. Furthermore, the proportion of monounsaturated fatty acid was higher in the T2, T3, and T1 groups vs. T0 rabbits, whereas the PUFA proportions were lower. It could be concluded that frass has great potential to be used as a partial substitute for rabbit diets.

The Effect of Early Feeding and Transporting Time from Hatcher to Farm On the Productive Performance and Carcass Traits of Broiler Chickens.

New researches suggest that an early feeding approach has a long-term influence on chick growth performance and nutrient metabolism. The present study was designed to determine the effect of early feeding and the time of chickens transferring from the hatchery to the field on broiler chickens' productive performance and carcass traits. Two hundred twenty-five chickens, one-day-old of broiler chickens Ross 308 with a mean live body weight of 45 g, were used and were randomly distributed to five treatments by 45 chickens per treatment with three replicates (15 chickens per replicate). The experimental treatments were as follows: T1 (control treatment) - the chickens were transferred 24 hours after hatching to the field without feeding, and in groups T2 to T5, the chickens were fed immediately and transferred to the field 24, 6,12,18 hours after hatching, respectively. The current results showed no significant (P>0.05) effects of the experimental treatments on final body live weight, weight gain, feed intake, and feed conversion ratio. In addition, the treatments' insignificant (P>0.05) effects on the weights of the carcass, abdominal fat, breast, thigh, back, wing, neck, heart, liver, and gizzard were found. It can be concluded that there was no evidence of a positive effect of early feeding and transporting duration after hatching on productive performance and carcass characteristics of broilers.

Effect of Neem Extract with Zinc Oxide Nanoparticles for Burn Wound Treatment

A study was conducted to determine the clinical efficiency of neem extract with Zinc Oxide (ZnO) nanoparticles on burn wound healing treatment in Rabbit model. Sixteen (16) male New Zealand white rabbits were assigned into four groups, each with four rabbits, and were kept together for 28 days. Group T0 as the control; T1 represented burn healing using neem extract; T2 represented burn healing using neem extract with ZnO nanoparticles; and T3 represented burn healing using a commercial drug (Bactrocin®). Data were accumulated on the healing rate, healing time, and WBC count. Healing rate was calculated at weekly interval and the healing time was observed regularly and calculated from day 7 at weekly interval and WBC count were investigated using an auto analyzer. The result showed that the healing rate (%) were T2 group= 93.75±1.25, T3 group=86.75±0.479, T1 group=84.25±1.75, and T0 group=70.75±2.25. Among this result the healing rate increased significantly in T2 (93.75±1.25) group, better by T3 (86.75±0.479), good by T1 (84.25±1.75) and worst by T0 (70.75±2.25) group. The application of neem extract with ZnO nanoparticles have also showed the quickest healing time (24.5±0.64 day) significantly (P&lt;0.01). At weekly interval WBC count (cells/µl) was varied significantly, the highest value was discovered in the T2 group. The study's overall findings demonstrated that using neem extract with ZnO nanoparticles resulted in faster wound healing with a shorter time requirement. It indicates that wound healing without the use of antibiotics was possible and using neem extract can be advised for the treatment of burn wounds.

Effect of dietary inclusion of rice distillers dried grains with solubles (rDDGS) on growth performance and production efficiency of Kuroiler chicken

Rice Distillers Dried Grains with Solubles (rDDGS) is a by-product of ethanol production which is used as alternative protein source in the poultry diet. Present investigation was conducted to assess the growth performance and production efficiency in Kuroiler chickens. Feeding trial was designed into four dietary groups, viz. T0 (Control, without rDDGS), T1 (7.5% rDDGS), T2 (15% rDDGS) and T3 (22.5% rDDGS) in different treatment groups along with basal corn soybean diet. Significant variations from 2nd to 8th weeks of age for body weight were found in all the dietary groups. Body weight gain differed significantly from 1st to 5th weeks among all the groups, however, overall (1-56d) body weight gain was found highest in T2 group (1640.29 g) followed by T1 (1572.52 g), T0 (1534.01 g) and T3 (1466.75 g). Feed intake showed significant variation from 2nd to 6th weeks of age. Maximum feed intake during entire length of trial was observed in the dietary group T2 and lowest in T3. Feed conversion ratio, feed conversion efficiency and protein efficiency ratio were not influenced significantly by feeding rDDGS. Hence, inclusion of rDDGS may be suggested as an alternate protein source and can replace soybean meal up to 15% without any detrimental effect on health and productivity.

Effectiveness of online self-leadership training on leaders’ self-leadership skills and recovery experiences

PurposeThe purpose of this study is to investigate the effectiveness of online self-leadership training (OSLT) in promoting leaders’ self-leadership skills and recovery experiences.Design/methodology/approachA non-randomized controlled trial was conducted under two conditions: a standardized seven-week OSLT (N = 43) and a control without any intervention (N = 42). All participants (N = 85) completed standardized questionnaires measuring self-reported self-leadership skills and recovery experiences. Additionally, participants in the intervention group were assigned to invite one team member each (N = 26) to assess their leaders’ pre-post self-leadership skills and pre-post leader–member exchange.FindingsSignificant interaction effects of time and group and increases in the OSLT group (t1 vs t2) in self-leadership skills (cognitive and natural reward strategies) and recovery experiences (detachment and relaxation) indicated the effectiveness of OSLT training. Significant improvements in self-leadership skills and leader–member exchange were reported by team members of leaders in the OSLT group.Originality/valueTo the best of the authors’ knowledge, this study was the first to examine the effectiveness of OSLT for leaders in business contexts in a controlled before-after intervention design. The findings of this study revealed improvements in self-leadership skills and recovery experience because of OSLT.

Potensi Getah Patikan Kebo (Euphorbia hirta L.) dalam Menurunkan Waktu Perdarahan pada Luka Kulit Tikus Putih (Rattus norvegicus)

Indonesia has many types of plants that are abundant and can be used as medicine. One of the plants used as medicine is patikan kebo (Euphorbia hirta L.) because it contains of several chemical compounds, which are tannin, flavonoid and saponin. Tannin and flavonoid are the main compounds in plants that help to stop the bleeding. The purpose of this study was to determine the potential of patikan kebo’s latex (Euphorbia hirta L.) to decrease the bleeding time in skin wounds of white rats (Rattus norvegicus). This research is a pre experiment. The number of experimental animals used was 10 which were divided into 2 treatment groups (T1 and T2). The treatment group T1)was not dripped with patikan kebo’s latex (Euphorbia hirta L.) and treatment group T2 was dripped with patikan kebo’s latex (Euphorbia hirta L.) and then the bleeding time was measured. Based on result of Independent Sample T Test showed that P value = 0,006 (P&lt;0,05), it means the bleeding time in skin wound of white rats that were dripped with patikan kebo’s latex and without dripped with patikan kebo’s latex are significant.