Pregnancy-related complications are the second global leading cause of death among women of reproductive age. In 2015, approximately 3,03,000 maternal deaths occurred worldwide, most of which could have been prevented. The application of evidence-based practices in labour at maternity units has shown changes in pregnancy and labour outcomes, hence the researcher undertaken this study. Methods: The research approach adopted for the present study was pre-experimental one group pre-test post-test research design. The 60 samples were selected by nonprobability purposive sampling technique. The self-structured knowledge questionnaires were used to gather the data. The data was analyzed by using the descriptive and inferential statistics. Result: In the pre-test the overall mean percentage of knowledge score was 50.66% with mean and SD of 15.20±1.48 and in post-test the mean percentage of post-test knowledge was 91.76% with mean and SD of 27.53±1.17. The mean difference was 12.33 with SD of 1.90. The calculated paired ‘t’ test value was 50.24 at the degree of freedom 59, the p value was 0.0001. There was no significant association between the demographic variables with pretest knowledge score. Interpretation and Conclusion: The video assisted teaching program was effective in enhancing the knowledge of the samples regarding evidence-based practices in labor. The study recommends to conduct a study on use of evidence-based practices among staff nurses in labor room.