Groups In Dendrogram Research Articles

COMPARISON OF CEMELE PEPPER WITH BELL PEPPER GENOTYPES (Capsicum annuum L. var. grossum) WITH RESPECT TO AGRONOMIC AND MORPHOLOGICAL CHARACTERISTICS

This study was conducted in 2014 and 2015 to compare Cemele pepper with other bell peppers (Capsicum annuum L. var. grossum) genotypes with respect to agronomic and morphological traits. Totally 75 bell peppper genotypes were collected from the centrum and villages of Kırşehir province. For a total of 48 agronomic and morphological characteristics of pepper genotype, the characterization study of IPGRI according to the criteria of the International Union of Plant Protection Preservation (UPOV) were done made. As a result of the principal component analysis, total 11 main component axes were obtained and these axes represented 73.25% of the total variance. Genotypes were divided into 15 groups in dendrogram according to morphological and agronomic characteristics. The mean of the quantitative characteristics of each group was determined and it was determined which group or group was the difference between them. As a result of cluster analysis; D1, D20, D54, D67K, D43 and D39 coded genotypes were determined to be the most distant genotypes in terms of agronomic and morphological degree of relation. To conclude, with the identification of the genotypes of bell peppers in Kırşehir province, it will provide significant advantages in future pepper breeding studies as well as contributing to the formation of pepper database.

Molecular diversity analysis using Simple sequence repeats (SSRs) in ‘A’ and ‘R’ lines of Ogura CMS system in Indian mustard

Fifty-eight SSR primers were used for molecular diversity analysis among 45 ‘A’ and ‘R’ lines (5 A lines and 40 restorers) of Indian mustard. Out of 58 primers 33 primers were found to be polymorphic. A total of 95 alleles were detected across all 45 genotypes. The PCR amplified products ranged from 70 bp to 350 bp in size. The number of alleles per locus varied from 2 to 5 with an average of 2.89. The PIC value varied from 0.206 to 0.749 with an average of 0.519. The highest PIC value was obtained for BG 132 (0.749) followed by BG 114 (0.744). The UPGMA cluster analysis suggested demarcation of 45 genotypes into six distinct groups in dendrogram wherein three groups had single genotype (restorer line) each. Cluster VI consisted of all five Ogura CMS lines only. In the remaining clusters, restorer lines were distributed. Based on similarity coefficient, cross combinations viz., RH 0749-OA & IOR-17, RH 0749-OA & IOR-20 and RH 119-OA & OR-17 were identified as diverse and might be suitable for using parent in heterosis exploitation in Indian mustard.

Bacterial communities of three plant species from Pb-Zn contaminated sites and plant-growth promotional benefits of endophytic Microbacterium sp. (strain BXGe71).

The endophytic bacterial community of two hyperaccumulators (Arabis alpine, Dysphania ambrosioides) and Veronica ciliate was investigated by Illumina sequencing technology. In addition, the culturable endophytic bacteria (EB) were isolated and their plant-growth promotion capabilities were studied. A dataset consisting of 221,075 filtered high-quality and classifiable unique 16S rDNA gene tags, and an average of 36,846 tags with a mean length of 464-bp for each sample was generated. In total, 10801 different operational taxonomic units (OTUs) were detected, belonging to 18 bacterial phyla, 41 classes, 91 orders, 135 families, and 215 genera. Pseudomonas was the most dominant genus in both shoots and roots of the two hyperaccumulators, making up 81.56% and 81.13%, 41.60% and 77.06% of the total number of OTUs, respectively. However, both Chao 1 and Shannon indices of EB of the two hyperaccumulators were significantly lower than those of V. ciliate (P <. 05), except the Shannon index of D. ambrosioides shoots. The endophytic bacterial community of roots and shoots of A. alpine showed greater similarity with that of D. ambrosioides roots (12 km away), and clustered to one group in dendrogram, in clear contrast to that of V. ciliate, which grew closer to A. alpine (60 m away). Combining results of soil and plant analyses, we suggest that the soil properties, especially heavy metal concentration, may influence the plants endophytic bacterial community composition. Pot experiments showed that the strain BXGe71 (Microbacterium sp.) from A. alpine significantly enhanced host plants' growth under multi-heavy metal (HM) stress (P < .05, t-test).