E Group Research Articles

Ovarian steroids modulate the systemic inflammatory response OF COWS challenged with lipopolysaccharide (LPS) intrauterine infusion

Postpartum uterine infections of dairy cows promote a local and systemic inflammation and interfere with reproductive efficiency. The aim of this study was to evaluate the effect of steroid hormones including progesterone (P4) and estradiol (E2) on the systemic inflammatory response of cows after being challenged with an intrauterine infusion of lipopolysaccharide (LPS). For this, a hemogram and serum dosage of haptoglobin (Hp) in eight primiparous Gir cows ovariectomized were performed on day (day 0) and after 24 h (day +1). Four cows (n = 4) were challenged (day 0) with 20 mL of 0.9% NaCl + 12.5 μg/kg LPS, and four cows (n = 4) were challenged (day 0) with 20 mL of 0.9% NaCl. For this, the study was divided in four experimental groups as: (1) Control group: without any hormonal treatment before day 0; (2) Group 24 h - E2: 1 mg of estradiol benzoate 24 h before (day −1); (3) Group 24 h - P4: 2.0 g of P4 device 24 h before (day −1); (4) Group 14 d - P4: 2.0 g of P4 device 14 days before (day −14). In the systemic response to LPS, there was an increase in Hp (control group; 24 h - P4 group; 14 d - P4 group), and on day +1 the Hp of 14 d - P4 group was higher when compared to the other groups. On day 0, the 14 d - P4 group had an increase in circulating leukocytes and lymphocytes cells than the control group (P < 0.01). On day +1 after LPS-challenge the 14 d - P4 group showed a decrease in circulating lymphocytes, eosinophils, and monocytes (P < 0.05). A neutrophilia with left shift in the two treatments with P4 (day +1), in addition to a thrombocytopenia and lower platelets compared to the 24 h - E2 group (P < 0.05) (day 0) were recorded. It was concluded that ovariectomized cows challenged with LPS, previously submitted to steroid hormones induce a systemic inflammatory response. Also, the systemic response is more intense after previous prolonged exposure to P4 and less intense after exposure to E2. This study provided important information relating the effect of ovarian steroids on the systemic inflammatory response of cows challenged with intrauterine LPS.

Impact of Watermelon Rind and Sea Buckthorn Meal on Performance, Blood Parameters, and Gut Microbiota and Morphology in Laying Hens

Natural ingredients from fruits and fruit-derived by-products have gained special interest as dietary supplements in poultry because of their health-promoting effects. The present work aims to evaluate the impact of the dietary inclusion of watermelon rind and sea buckthorn meal on performances, blood parameters, and gut microbiota and morphology of laying hens. A 4-week trial was conducted on 90 Tetra SL layers (32 weeks old), assigned to three dietary treatments (C, E1, and E2). The C group hens were fed a basal diet based on corn-soybean meal. The experimental diets included 10 g of watermelon rind/kg fed (E1) and 20 g of sea buckthorn meal/kg fed (E2). During the feeding trial, we monitored the performances. Blood samples, intestinal tissue, and intestinal content were collected to assess the effect of dietary ingredients on health status, digestive enzyme activity, intestinal morphology, and gut microbiota characterization. From the blood samples, cholesterol and triglycerides significantly (p &lt; 0.05) decreased in E1 and E2 compared with the C group. Dietary watermelon rind and sea buckthorn meal positively impacted villus height. Maltase and invertase activity increased only in the duodenum of the E2 group, while alpha-amylase decreased in the duodenum and jejunum of both E1 and E2 groups. The two supplements tested triggered Firmicutes and Lactobacillus spp. multiplication, while reducing harmful bacteria such as Bacteroidetes and Enterobacteriaceae. The study provides the first evidence that the dietary inclusion of watermelon rind and sea buckthorn meal can be used in laying hens’ diets with a beneficial impact on hens’ biochemical parameters, gut microbiota, and gut morphology.

APOE Polymorphism and Endocrine Functions in Subjects with Morbid Obesity Undergoing Bariatric Surgery.

Background: Obesity is an interplay between genes and the environment, including lifestyle. The genetics of obesity is insufficiently understood. Apolipoprotein E (APOE) genetic polymorphism has been associated with a wide range of disorders. Knowing that some APOE alleles are associated with obesity and endocrine disorders that are common in obesity, the present study aimed at exploring associations between APOE polymorphisms and endocrine functions in subjects with obesity undergoing bariatric surgery. Methods: Analyses of hormones in blood collected before and one year after bariatric surgery were examined. The APOE alleles were grouped as follows: E2 = ε2ε2 + ε2ε3; E3 = ε3ε3 + ε2ε4; E4 = ε3ε4 + ε4ε4. The APOE groups were analysed as nominal and ordered groups (E2-E3-E4) with a linear mixed model to predict the hormonal effects of the groups. Results: Forty-nine women (79%) and thirteen (21%) men with a mean age of 47.7 (SD 8.5) years were included in the study. The adiponectin level was significantly lower (p < 0.05) in the E2 group compared with the E4 group. Adiponectin and cortisol were positively and negatively associated, respectively, with the ordered APOE groups. Conclusions: The ordered APOE groups E2-E3-E4 were significantly associated with high and low levels of adiponectin and cortisol, respectively. The findings indicate APOE-mediated effects on body weight and metabolic functions in subjects with morbid obesity.

Imbalance in the estrogen/androgen ratio may affect prostate fibrosis through the TGF-β/Smad signaling pathway.

The present study aimed to investigate the effects of an imbalance in the estrogen/androgen ratio on prostate fibrosis. Different concentrations of dihydrotestosterone (DHT) or estradiol (E2) dissolved in corn oil were injected subcutaneously into the nape of the castrated Sprague-Dawley (SD) rats over 28 consecutive days. Masson's trichrome staining and immunohistochemical staining were performed to detect the content of collagen fibers and the expression of collagen I, fibronectin, and elastin in the rat prostate of each group, respectively. DHT + E2 at different concentrations was administered to human normal prostate stromal immortalized cells (WPMY-1 cells) for 1week. The expression of collagen I, fibronectin, elastin, transforming growth factor-β1 (TGF-β1), Smad3, and Smad7 was detected by Western blotting (WB). Then, WPMY-1 cells treated with 10nM DHT + 5pM E2 were incubated with the TGF-β/Smad pathway inhibitor SD208 for 1week, after which collagen I, fibronectin, and elastin expression was detected by WB. Compared with the uncastrated control and corn oil injection groups, the collagen fiber content and collagen I and fibronectin expression were increased and elastin expression was decreased in the castrated rat prostate with corn oil injection group (p < 0.01). Compared to castrated corn oil injection group, collagen fiber content, collagen I, and fibronectin expression were significantlydecreased, and elastin expression was significantly increased in the castrated rat prostate 0.15mg/kg DHT treatment group (p < 0.01). Following treatment with 0.15mg/kg DHT, the content of collagen fibers, and the expression of collagen I and fibronectin were increased, and the expression of elastin was decreased in the rat prostate with increasing concentrations of E2 treatment group compared to the 0.15mg/kg DHT group (p < 0.05, p < 0.01). Following treatment with 0.05mg/kg E2, the collagen fiber content and the expression of collagen I and fibronectin were decreased, and the expression of elastin was increased in the rat prostate with increasing DHT concentration treatment group compared to the 0.05mg/kg E2 group (p < 0.05, p < 0.01). Compared with the Control group, the expression of collagen I, fibronectin, TGF-β1 and Smad3 was decreased, and the expression of elastin and Smad7 was increased in WPMY-1 cells after treatment with 10nM DHT (p < 0.01). Following treatment with 10nM DHT, the expression of collagen I, fibronectin, TGF-β1, and Smad3 was increased, and the expression of elastin and Smad7 was decreased in WPMY-1 cells with increasing E2 concentration treatment compared to the 10nM DHT group (p < 0.05, p < 0.01). Following treatment with 5pM E2, the expression of collagen I, fibronectin, TGF-β1, and Smad3 was decreased, and elastin and Smad7 expression was increased with increasing DHT concentration compared to the 5pM E2 group (p < 0.05, p < 0.01). Compared to the 10nM DHT + 5pM E2 group, the expressions of collagen I and fibronectin were decreased; the expression of elastin was increased in WPMY-1 cells after the supplement of TGF-β/Smad pathway inhibitor SD208 group (p < 0.05, p < 0.01). An imbalance in the estrogen/androgen ratio may affect prostate fibrosis. E2 may activate the degree of prostate fibrosis. In contrast to the effect of E2, DHT may inhibit the degree of prostate fibrosis, which might involve the TGF-β/Smad signaling pathway.

ANTIOXIDANT ACTIVITY LEAVES KATIMAHA (Kleinhovia hospita L.)

The free radicals that people face in their daily activities require humans to get additional antioxidants from the outside. To overcome this problem, it is necessary to look for many sources of natural antioxidants considering the need for antioxidants that are increasingly needed Katimaha (K. hospita L.) is believed to have many benefits in the field of medicine. Therefore, katimaha has the potential to be used as a source of natural antioxidants. The research methods carried out were refining katimaha leaf simplicia, extraction, fractionation, antioxidant activity test with TLC plate, purification, determination of compound class, and antioxidant activity test using the DPPH method. The results showed that the active fraction of Katimaha leaf was the n-hexane fraction and the ethyl acetate fraction. Column chromatography on the active fraction found six pure eluates that had antioxidant activity, namely N1, N3, N5, E1, E2, and E4. Groups of compounds N1, N3, N5, E1 are terpenoids, group E2 eluates are tannins, while E3 eluates are flavonoid compounds. The pure eluate antioxidant activity test using the DPPH method obtained IC50 values of N1 are 40,142 ppm, N3 80,057 ppm, N5 271,643 ppm and E1 89,16 ppm, E2 124.91 ppm and E4 52.51 ppm.

Pregnancy and brain architecture: Associations with hormones, cognition and affect.

Sex hormones such as estradiol (E2) have long-lasting influence on brain architecture. Recent studies indicate further structural changes during hormonal transition periods including pregnancy, when women experience the greatest increase in sex hormone levels across their life span. In the present study, three groups of women (n=44) with different levels of E2 underwent structural magnetic resonance imaging: (1) first-time pregnant women (n=13, 'extreme E2group'); (2), nulliparous, naturally cycling women who received 12mg of E2valerate (n=16, 'high E2group'); and (3) nulliparous, naturally cycling women receiving a placebo and hence low E2 (n=15, 'low E2group'). Blood samples were taken to assess hormonal levels. Moreover, parameters for cognition, emotion regulation and affect were assessed. On the neuronal level, the extreme E2 compared to the high E2group showed a reduced gray matter volume in the left putamen. However, no significant differences were found between the low vs. high E2 groups, nor between the low E2 and extreme E2groups. Cognitive performance was reduced in the extreme E2group, although a positive affect was increased compared to the high E2 and low E2groups. Furthermore, regression analyses revealed several associations between cognition, subjective measures of affect, emotion regulation and gray matter volume. A volume reduction of the left putamen during pregnancy further supports the notion that the female brain is shaped by hormonal transition phases, possibly preparing women for their future roles (e.g., pregnant women for their role as mothers).

Doprinos primene direktne u odnosu na indirektnu hands-on instrukciju na postignuća učenika u početnom obrazovanju u prirodnim naukama

Ensuring the optimal (adequate) level of instructional guidance in teaching natural sciences in primary education is of utmost importance for the proper acquisition and application of knowledge. The goal of this paper is to examine the contribution of direct hands-on instruction relative to an indirect one on student achievement regarding the movement and properties of materials in classes of integrated natural sciences (classes of the World around Us and Science and Social Studies). The research tasks include a comparative analysis of the quality of students' knowledge acquired with the application of direct in relation to indirect hands-on instruction, as well as the analysis of the permanence of that knowledge. The following methods were used in the research: theoretical analysis method, descriptive-analytical method, and experimental method. The research technique is testing, and the instrument is knowledge tests (pre-test, post-test, and re-test). The research sample consisted of 94 students of the third grade of primary school, divided into two groups: E1 (application of indirect hands-on instruction) and E2 group (application of direct hands-on instruction). The results of the research indicate that the students of the E1 group achieved somewhat better and more lasting knowledge about the movement and properties of materials in relation to the students of the E2 group. However, a significant difference between their achievements was observed only at the cognitive level evaluated in the post-test. This leads to the conclusion that both types of hands-on instructions should be applied in the third grade when teaching the content related to the movement and properties of materials. In addition, greater preference should be given to indirect over direct hands-on instruction because it not only provides an opportunity for students to have better achievement, but also it enables a greater degree of independent research and experimental work.

THE PROFILE OF FATTY ACIDS AND THE EGGS QUALITY FROM HENS FED TO THE DIET WITH FLAX SEEDS, RAPESEED MEAL AND VITAMIN E SUPPLEMENTS

This experiment investigates the effect of flaxseed meal, rapeseed meal and vitamin E supplementation, when used together, on the production parameters of fatty acid composition and eggs quality characteristics in Tetra SL laying hens (38 to 46 weeks of age). For this, 120 hens were allocated one of three treatments, with 40 hens in each group. The hens were fed either a control diet (C), a control diet including 2.5 % flaxseed meal and 2.5% rapeseed meal (E1) or 2.5% flaxseed meal and 2.5% rapeseed meal with 73 mg/ kg feed of vitamin E as an antioxidant supplement (E2) for 8 weeks. Hens consuming the E1 diet had greater egg production and egg mass than those from group C. The feed conversion ratio in both the E1 and E2 groups was lower (P &lt; 0.05) than in the C diet group. E2 had considerably higher egg weight, albumen pH, yolk pH and Haugh unit than E1 and C (P0.05), as a response to the vitamin E antioxidant effect. The most α-linolenic fatty acid content (1.07 g FAME) was found in eggs produced by chickens fed the E2 diet, (1.07 g FAME), followed by E1 (0.91 g FAME), with both being significantly higher than C eggs (0.23g FAME). Furthermore, all n-6 studied fatty acids concentrations were significantly lower (P &lt; 0.05) in E1 and E2, while all n-3 fatty acids concentrations were significantly greater (P &gt; 0.05) in E1 and E2. When comparing the n-6/n-3 ratio of fatty acids from experimental treatments (6.44 and 6.74) with C treatment (18.19), a significant difference was observed (almost 65% lower).

Dose and time-dependent effects of glucocorticoid: A morphologic and morphometric study in the broiler lung

Glucocorticoids (GCs) play a critical function in neonatal lung growth and maturation though the long-term dose-dependent effects of GCs on lungs are still ambiguous. The purpose of the present study is to explore the morphologic and morphometric changes in broiler lungs in reaction to dietary GC. Day-old chicks (DOCs) were purchased and divided into four groups at random i.e. one control group (C) and three experimental groups (E1, E2, and E3). Commercial broiler feed was provided to both the control and experimental groups where the feed of the experimental groups contained GC, dexamethasone (DEX) at the rate of 3, 5, and 7 mg/ kg in E1, E2, and E3 groups, respectively. Lung (left) samples were collected on days 7, 14, 21, and 28. After recording the gross morphologic and morphometric data, the tissue samples were processed for histological investigation. In the gross morphological study, congested lung with reddish to blackish discoloration was seen in experimental broilers. The gross morphometric study revealed a significant decrease in weight, length, and width of the lung. In the histomorphologic study, the lung of experimental broilers revealed vascular and parabronchial lumen congestion. Mild to the severe edematous lesion was also found evident along with the absence of distinguishable inter parabronchial septa. Degeneration of lung parenchyma with the appearance of lipid droplets, inflammatory cell infiltration, and fibrosis was seen to some extent. The histomorphometric investigation revealed a significant decrease in the diameter of the parabronchus as well as the thickness of the parabronchial wall. The study results suggest that dexamethasone may adversely alter the gross and histological characteristics of broiler lungs.

Effect of Diet Supplementation with Enterococcus Durans ED26E/7 and its Durancin ED26E/7 on Growth Performance, Caecal Enzymatic Activity, Jejunal Morphology and Meat Properties of Broiler Rabbits

Abstract The present study investigates the effects of Enterococcus durans ED26E/7 beneficial strain and its enterocin – durancin (Ent) ED26E/7 on selected parameters in rabbits: growth performance, caecal enzymatic activity, jejunal morphometry and meat physico-chemical characteristics. Seventytwo rabbits (aged five weeks, M91 meat line, both sexes) were divided into experimental groups E1 (E. durans ED26E/7 strain; dose 500 μL/animal/day, concentration 109 CFU/mL) and E2 (durancin EntED26E/7; dose 50 μL/animal/day, with activity 12 800 AU/mL) and control group (C). The additives were administered in drinking water for a period of 21 days. All animals remained in good health during the experiment. The highest body weight gain (increase by 1.5% compared to C) was noted in E1 group during ED26E/7 strain application (P&lt;0.001). Both bioactive compounds positively influenced (reduced) the feed conversion ratio (P&lt;0.001). The ED26E/7 strain and its EntED26E/7 application stimulated the activity of most enzymes tested in the caecum; only the amylolytic and inulolytic activity in E2 group decreased during durancin ED26E/7 addition. Both additives, but mainly the ED26E/7 strain, showed a tendency to improve the jejunal morhological parameters till the end of the experiment (day 42). The meat physico-chemical parameters were not negatively influenced by the application of E. durans ED26E/7 strain and its durancin ED26E/7. The diet supplementation with bacteriocinogenic and probiotic E. durans ED26E/7 strain and its EntED26E/7 may improve the growth performance, caecal enzymatic activity and jejunal morphometry of rabbits, without any negative effect on rabbit meat quality.

Analysis of APOE and SLCO1B1 Gene Polymorphism and Correlation with Dyslipidemia in China.

The aim of the study was to analyze the polymorphism of APOE and SLCO1B1 genotype and correlation with blood lipid levels in patients with dyslipidemia in Hunan Province, China. Concentration of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were retrospectively analyzed from 396 patients with dyslipidemia from 2016 to 2020. The APOE and SLCO1B1 genotypes were detected by PCR-fluorescent probe. SPSS21.0 software was used to analyze the gene frequency and their correlation. The frequency of E3/E3 genotype was the highest in the APOE gene, reaching 64.14%. The concentrations of LDL-C and TC in E4 phenotype group were the highest and were statistically different from those in E2 and E3 phenotype groups (p < 0.05). There were significant differences in E4 phenotype between the dyslipidemia group and the normolipidemia group (p < 0.05). The frequency of *1b/*1b genotype was the highest in the SLCO1B1 gene, reaching 41.41%. The concentrations of TC and LDL-C in the intermediate OA1P1B1 function group were higher than those in the normal OA1P1B1 function group and the differences were statistically significant (p < 0.05). The genotypes of APOE and SLCO1B1 gene are correlated with concentration of TC and LDL-C, and their genotypes frequency distribution have regional differences.

Fecal Microbial Enterotypes Differentially Respond to a High-fat Diet Based on Sex in Fischer-344 Rats.

The gut microbiota interacts with the host gut environment, which is influenced by such factors as sex, age, and host diet. These factors induce changes in the microbial composition. The aim of this study was to identify differences in the gut microbiome of Fisher-344 (F344) rats fed a high-fat diet (HFD), depending on their age and sex. Fecal microbiomes from 6-, 31-, and 74-week-old, and 2-year-old both male and female rats (corresponding to 5-, 30-, 60-, and 80-year-old humans) were analyzed using 16S rRNA gene sequencing, phylogenetic investigation of communities by reconstruction of unobserved states, and enterotype (E) assessment. Moreover, the effect of an HFD on colonic epithelial cells was measured using real-time quantitative PCR. Alpha diversity decreased in the HFD group regardless of age and sex. Based on the enterotype clustering of the whole fecal microbiome, clusters from male rats were divided into E1 and E2 enterotypes, while clusters from female rats were divided into E1, E2, and E3 enterotypes. The female E3 group showed a significantly high abundance in the Ruminococcus genus and expression of Tlr2 mRNA, which may reflect compensation to the HFD. Moreover, the female E3 group showed a lower ratio of opportunistic pathogenic strains to commensal strains compared to the female E2 group. Administration of an HFD influenced the rat fecal microbiota in all assessed age groups, which could be further differentiated by sex. In particular, female rats showed a compensatory enterotype response to an HFD compared to male rats.

Sitagliptin ameliorates hypoxia-induced damages in endometrial stromal cells: an implication in endometriosis

ABSTRACT Hypoxia-induced damage in endometrial stromal cells (ESCs) is an important event in the pathological progression of Endometriosis. It is reported that significant inflammation is induced by hypoxia in ESCs, mediated by serval inflammatory progressions, pathways, or factors. Sitagliptin, an important member of the dipeptidyl peptidase-4 (DPP-4) inhibitors family and has been widely used for the management of type 2 diabetes. It has been recently reported to exert significant anti-inflammatory effects. Here, we aim to assess whether Sitagliptin possesses a protective effect against hypoxia-induced damages in ESCs. Our findings indicate that exposure to hypoxia significantly increased oxidative stress in ESCs by increasing the production of reactive oxygen species (ROS) and decreasing the levels of reduced glutathione (GSH), which was ameliorated by Sitagliptin. Additionally, the excessively produced inflammatory mediators, including tumor necrosis factor (TNF)-α, interleukin (IL)-6, monocyte chemoattractant protein-1 (MCP-1), cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE2), and high mobility group box (HMGB)-1, in hypoxia-treated HESCs were pronouncedly repressed by Sitagliptin. The activated p38 mitogen-activated protein kinases (MAPK) pathway was observed in hypoxia-stimulated HESCs, then greatly inhibited by the introduction of Sitagliptin. Lastly, hypoxia-induced phosphorylation and degradation of IκBα, as well as the upregulation of nuclear factor kappa-B (NF-κB) p65 and increased transcriptional activity of NF-κB, were dramatically abolished by Sitagliptin. Collectively, Sitagliptin ameliorated hypoxia-induced damages in ESCs by suppressing the inflammation.

GnRH-a-Induced Perimenopausal Rat Modeling and Black Cohosh Preparations' Effect on Rat's Reproductive Endocrine.

GnRH-a-Induced Perimenopausal Rat Modeling and Black Cohosh Preparations' Effect on Rat's Reproductive Endocrine.

Effect of liver cirrhosis on erectile function in rats: A study combining bioinformatics analysis and experimental research.

To explore the mechanism through which liver cirrhosis (LC) causes erectile dysfunction (ED). Bioinformatic analysis was used to predict the potential signalling pathways in LC-induced ED, and N-nitrosodiethylamine was used to establish a rat model of LC. H&E staining, Western blotting and RT-qPCR were used to detect pathological tissue damage and changes in mRNA and protein expression levels. In addition, the expression levels of sex hormones such as estradiol (E2), prolactin (PRL) and testosterone (T) were measured. The hypoxia-inducible factor (HIF) pathway was an important pathway in our bioinformatics prediction. Pathological damages were detected in the liver and penile tissues of the model rats. Compared with the normal group's serum hormone levels, E2 and PRL were increased in LC rats, while T was decreased (p<0.01). The mRNA and protein expression results from penis tissues showed that endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) were both downregulated, and HIF-1α was upregulated in the model group compared to the normal group (p<0.01). These data suggest that LC hinders erectile function and causes histopathological changes in the penis by affecting the expression of HIF-1α, eNOS, iNOS, E2, PRL and T.

Taux d’usure et ostéolyse avec deux types de polyéthylène recuit hautement réticulé de deuxième génération en arthroplastie totale de la hanche : étude comparative rétrospective au recul minimum de cinq ans

BackgroundAs no previous study has directly compared the linear wear rate in two types of second-generation annealed highly cross-linked polyethylene, we performed a retrospective study with a minimum of 5-year follow-up to assess primary arthroplasties in the: (1) wear rates; and (2) incidence of osteolysis of the two types of HXLPE. HypothesisThere was no significant difference in the linear wear rate and the incidence of osteolysis between the two types of second-generation annealed highly cross-linked polyethylene. Patients and methodsIn this single-center study, we reviewed 257 cases of primary cementless total hip arthroplasties between 2011 and 2015, which were performed with 32mm delta ceramic on second-generation annealed highly cross-linked polyethylene (X3 and E1 were used in 105 and 103 cases, respectively). The mean wear rate was evaluated using a computer-assisted method, and the incidence of osteolysis was evaluated based on the appearance of a localized area with loss of trabecular bone or cortical erosion adjacent to the implants during the latest follow-up. ResultsIn total, we evaluated 208 cases, followed postoperatively for over 5 years (mean, 6.1 years, range: 5.0–8.0). There were no significant differences between the two groups with respect to age (list in order of group X, group E, p-value) (61.2±12.3, 62.7±12.1, p=0.36), sex (ratio of male: 17.1 %, 14.6 %, p=0.61), body mass index (22.9±3.7, 22.8±4.0, p=0.91), pre- (49.9±14.8, 48.5±13.8, p=0.49) and postoperative (91.3±9.1, 92.7±7.0, p=0.23) Japanese Orthopaedic Association Hip Score, cup size (50.8±3.0, 50.9±2.2, p=0.70), cup inclination (38.7±4.8, 37.6±4.8, p=0.10), and cup anteversion (18.7±6.9, 18.5±7.6, p=0.80). The mean linear wear rates of the X3 and E1 groups were 0.057±0.039 (range: 0–0.16) and 0.054±0.037mm/year (range: 0–0.15), respectively (p=0.61). No osteolysis was found on the final plain radiographs in both groups. DiscussionThis study revealed that both types of highly cross-linked polyethylene have excellent linear wear rates and were equally safe to use. However, the difference between the two materials in terms of the long-term wear rate should be further validated. Level of evidenceIII; retrospective case control study.

Effect of Different Concentrations of Esmolol on Perioperative Hemodynamics and Analgesia in Patients Undergoing Colectomy: A Prospective, Randomized Controlled Study.

PurposeThe aim of this study was to investigate the efficacy of esmolol on intraoperative hemodynamic and perioperative analgesic management.MethodsTotally, 125 patients undergoing colectomy were randomly divided into three groups. Group S (saline group) was administered 0.75 mL/kg/h of normal saline for 5 min before anesthesia induction and maintenance of 0.25 mL/kg/h; Group E1 and Group E2 were administered 0.5 mg/kg and 1.0 mg/kg esmolol for 5 min before anesthesia induction, and maintained of 0.5 mg/kg/h and 2.0 mg/kg/h, respectively. Several parameters including indexes of hemodynamics variation (primary outcome), intra- and postoperative analgesic usage, and pain score were measured.ResultsGroup E1 and Group E2 had significantly lower intubation response than Group S (P = 0.007, P = 0.001), and extubation response of Group E2 was significantly lower than Group S (P = 0.007). The opioid consumption in Group E1 and Group E2 was significantly lower than in Group S intraoperatively (P = 0.020 and 0.007). The incidence of postoperative adverse reactions among the three groups was not statistically significant (P = 0.368 and 0.772).ConclusionEsmolol 0.5 mg/kg and 1.0 mg/kg infusion before intubation both can effectively inhibit the intubation response, while only maintenance with 2.0 mg/kg/h of esmolol can reduce the incidence of extubation response. At the same time, esmolol can decrease intraoperative opioid requirement without increasing the risk of adverse reactions.Trial RegistrationChiCTR1900024538 and the date of registration was July 15, 2019 at http://www.chictr.org.cn.

Effects of High Fiber Ingredients with Enzyme/Phytoadditive Addition on Laying Hens Productive Performances, Egg Nutritional Quality and Intestinal Morphology

Abstract Fibre inclusion in laying hen diets it is considered a solution to reduce feed formulation costs by improving nutrients absorption and metabolization within the gastrointestinal tract. A 5-wk study was conducted on 200, Tetra SL layers (28 weeks) randomly allocated in 5 groups (C, E1, E2, E3, E4, respectively), with initial average weights of 1732.26±109.35 g/hen, housed (4 hens/cage; 10 cages/group; 40 hens/group). All groups had the same basic feed compound structure (corn, oil, soybean meal and sunflower meal). The conventional diet (C group) contained 2780 kcal/kg Metabolizable energy (ME); 17.5% Crude protein (CP); 4.39% Crude fibre (CF). The experimental diets added 16.56% sunflower meal and 20% rice bran, increasing the dietary cellulose up to 7%. The fibre digestibility was improved using on E2 (0.015% enzyme); E3 (0.015% phytoadditive) and E4 (0.015% enzyme + 0.015% phytoadditive). The high fibre diets had no negative impact on productive performances and egg quality parameters. Yolk cholesterol significantly decreased (P&lt;0.05) on E1, E3 and E4 groups compared to C, E2 groups. Villi height increased significantly (P&lt;0.05) on E2 group compared to all other groups. In conclusion, the high dietary crude fibre (7%), additional enzymes/phyto additives, can improve the nutrient digestibility and intestinal morphology with beneficial effects on health and egg quality.

Ulmoplus® Increases FGF-2 Expression and Promote Burn Wound Healing

The role of fibroblast growth factor (FGF) in the wound repair process has been described, particularly during the stimulation of angiogenesis in the proliferative phase. Ulmo honey (Eucryphia cordifolia) has demonstrated important bactericidal, fungicidal, antioxidant and healing properties. Ulmoplus®, a honey-based medicinal product, accelerates healing time and promotes fibroblast activation and collagen fiber production. The aim of this study was to evaluate the immunohistochemical expression of endogenous FGF-2 in burns treated with Ulmoplus® vs. the gold standard (hydrogel-tull), and Ulmo honey as the first step to clarifying the regulatory mechanism of this therapeutic option. 15 adult guinea pigs (Cavia porcellus) were used, divided into three groups: C+: positive control treated with hydrogel-tull (gold standard); E1: experimental group treated with Ulmo; and E2: experimental group treated with Ulmoplus®. A deep uniform burn was made under anesthetic that covered 1 cm2 of the skin on the back of each animal. The daily treatments were administered with hydrogel-tull, Ulmo honey or Ulmoplus®, where appropriate. On treatment day 10 the biopsies were taken and processed for histological and immunohistochemical analysis. The immunolabeling was quantified through an integrated optical density (IOD) analysis, expressed as lum/ µm2 using the Image- ProPremier 9.1 software. The endogenous expression of FGF-2 was consistent with the stage of healing presented by the study groups, varying in its localization and IOD intensity. The E2 group presented a significantly higher IOD (60053.55 ± 59877.84 lum/µm2) than the C+ (p = 0.001) and E1 (p = 0.001) groups. Our findings indicated that the medicinal Ulmoplus® honey is effective at facilitating wound closing, and the promoter effect of FGF-2 expression accelerated the healing process compared to the treatments with hydrogel-tull (gold standard) and Ulmo honey.

The effects of corrective gymnastics on the postural status of the spine in the frontal plane in preschool children

&amp;lt;p&amp;gt;Postural irregularities, i.e., deviations from normal posture, are a growing problem today. Physical inactivity in preschool children can lead to some physiological changes, which, if not remedied timely, tend to more seriously impair health in later stages of life. Muscle activity caused by biomechanical adaptation to certain body positions in a sedentary regime in children can form muscle asymmetries. The study aimed to determine the effects of corrective gymnastics on the postural status of the spine in the frontal plane in preschool children. The total sample of children (n=133; AGE: 6.2&amp;amp;plusmn;0.6) was divided into three sub-samples: experimental group E1 &amp;amp;ndash; 45 (25.57%), experimental group with additional exercises E2 &amp;amp;ndash; 45 (25.57%) and control group C-43 (48.86%). This is a longitudinal-type study that involved the application of a ten-week corrective gymnastics intervention on children aged 5 to 7 from Subotica, who participated in the formation of the sample. Based on the analysis of the results of the multivariate analysis of variance it can be concluded that there are no statistically significant differences (P=0.17) between the subjects from the experimental groups and the control group in the variables used for assessment of the spinal column in the frontal plane, and it is necessary to extend the treatment time in preschool children when corrective gymnastics is applied to changes in postural status when it is observed in the frontal plane.&amp;lt;/p&amp;gt;