Ground Wheat Research Articles

Postbiotics from Saccharomyces cerevisiae fermentation stabilize microbiota in rumen liquid digesta during grain-based subacute ruminal acidosis (SARA) in lactating dairy cows

BackgroundSubacute ruminal acidosis (SARA) is a common metabolic disorder of high yielding dairy cows, and it is associated with dysbiosis of the rumen and gut microbiome and host inflammation. This study evaluated the impact of two postbiotics from Saccharomyces cerevisiae fermentation products (SCFP) on rumen liquid associated microbiota of lactating dairy cows subjected to repeated grain-based SARA challenges. A total of 32 rumen cannulated cows were randomly assigned to 4 treatments from 4 weeks before until 12 weeks after parturition. Treatment groups included a Control diet or diets supplemented with postbiotics (SCFPa, 14 g/d Original XPC; SCFPb-1X, 19 g/d NutriTek; SCFPb-2X, 38 g/d NutriTek, Diamond V, Cedar Rapids, IA, USA). Grain-based SARA challenges were conducted during week 5 (SARA1) and week 8 (SARA2) after parturition by replacing 20% DM of the base total mixed ration (TMR) with pellets containing 50% ground barley and 50% ground wheat. Total DNA from rumen liquid samples was subjected to V3–V4 16S rRNA gene amplicon sequencing. Characteristics of rumen microbiota were compared among treatments and SARA stages.ResultsBoth SARA challenges reduced the diversity and richness of rumen liquid microbiota, altered the overall composition (β-diversity), and its predicted functionality including carbohydrates and amino acids metabolic pathways. The SARA challenges also reduced the number of significant associations among different taxa, number of hub taxa and their composition in the microbial co-occurrence networks. Supplementation with SCFP postbiotics, in particular SCFPb-2X, enhanced the robustness of the rumen microbiota. The SCFP supplemented cows had less fluctuation in relative abundances of community members when exposed to SARA challenges. The SCFP supplementation promoted the populations of lactate utilizing and fibrolytic bacteria, including members of Ruminococcaceae and Lachnospiraceae, and also increased the numbers of hub taxa during non-SARA and SARA stages. Supplementation with SCFPb-2X prevented the fluctuations in the abundances of hub taxa that were positively correlated with the acetate concentration, and α- and β-diversity metrics in rumen liquid digesta.ConclusionsInduction of SARA challenges reduced microbiota richness and diversity and caused fluctuations in major bacterial phyla in rumen liquid microbiota in lactating dairy cows. Supplementation of SCFP postbiotics could attenuate adverse effects of SARA on rumen liquid microbiota.

Taste Preferences in Broilers: Effect of Age, Delivery Matrix, and Number of Chickens per Pen on Selection and Consumption Behaviour

Due to substantial differences between studies, the understanding of avian taste perception remains incomplete. Also, studies on chicken taste preferences have mainly focused on measuring consumption differences, neglecting consumption behaviour patterns. This study investigated how age, the compound delivery matrix, and the number of birds per pen affect broiler chicken preferences and consumption behaviour, and established their preference values for four taste compounds. Ninety-six one-day-old male broiler chickens (Ross 308) were divided into two age groups (initial: days 7–23; final: days 26–42), with two compound delivery matrices (water or ground wheat) and two numbers of birds (one or two chickens per pen), following a 2 × 2 × 2 factorial design. Four taste compounds (sucrose, monosodium glutamate (MSG), L-lysine, and calcium carbonate) were tested at different concentrations. Preferences were assessed at 2, 4, and 8 h post-test, along with recording various behavioural parameters. Initial-stage birds showed higher (p < 0.001) preference values, time of approach (TA), number of bouts (NB), duration of bouts (DB), and number of pecks (NP) than final-stage birds. Birds exposed to a water matrix also exhibited higher (p < 0.001) preference and NB, while those exposed to a ground wheat matrix showed a higher (p < 0.001) NP. Pens with a pair of birds had a higher (p < 0.003) 2 h preference, TA, NB, DB, and NP, than pens with a single chicken. Chickens showed significant preference values for 100 mM sucrose at 2 h (p = 0.025), 150 mM MSG at 4 h (p = 0.026) and 8 h (p = 0.013), and 300 mM MSG at 2 h (p = 0.013). We concluded that all the variables evaluated influence broilers’ taste preferences and consumption behaviour during selection tests. Future studies should prioritize including chickens in the initial stage of the production cycle, testing them in pairs or groups, and delivering compounds via a liquid matrix.

Karakteristik Kimia dan Sensori Crackers dengan Penambahan Tepung Campolay (Pouteria campechiana) dan Teping Ikan Teri Nasi (Stolephorus sp)

ingredients can be added to the manufacture such as campolay flour and ground wheat flour to increase the nutritional content of the crackers. This study aims to make crackers with the addition of campolay flour and rice anchovy flour as a form of food diversification and to find the selected formulation from the ratio of wheat flour: campolay flour: rice anchovy flour. This study used a completely randomized design (CRD) with one treatment factor, namely the ratio of wheat flour : campolay flour : anchovy flour (80:15:5), (70:20:10), (60:25:15), ( 50:30:20). Product analysis includes chemical analysis of water content, acid insoluble ash content, and protein content as well as sensory and hedonic quality tests as a determinant of selected products. The selected treatment was then subjected to further chemical analysis including calcium levels and beta-carotene levels. Data analysis and research used (ANOVA) with Duncan's further test with a 95% confidence interval. The results showed that the selected crackers were treated with a ratio of wheat flour: campolay flour: anchovy flour (70:20:10) with a quality that did not smell of campolay flour, smelled of anchovy rice flour, had a yellow color, had a textured crunchy and salty taste. Crackers had a moisture content of 4,89%, acid insoluble ash content of 0,11% and protein content of 13,28%, an average calcium content value of 4339,19 mg/100g and an average value of beta-carotene content of 1,015 mg/kg

A disposable immunosensor array using cellulose paper assembled chemiresistive biosensor for simultaneous monitoring of mycotoxins AFB1 and FB1

Due to the common contamination of multiple mycotoxins in food, which results in stronger toxicity, it is particularly important to simultaneously test for various mycotoxins for the protection of human health. In this study, a disposable immunosensor array with low-cost was designed and fabricated using cellulose paper, polydimethylsiloxane (PDMS), and semiconducting single-walled carbon nanotubes (s-SWCNTs), which was modified with specific antibodies for mycotoxins AFB1 and FB1 detection. The strategy for fabricating the immunosensor array with two individual channels involved a two-step protocol starting with the form of two kinds of carbon films by depositing single-wall carbon nanotubes (SWCNTs) and s-SWCNTs on the cellulose paper as the conductive wire and sensing element, followed by the assembly of chemiresistive biosensor with SWCNTs strip as the wire and s-SWCNTs as the sensing element. After immobilizing AFB1-bovine serum albumin (AFB1-BSA) and FB1-bovine serum albumin (FB1-BSA) separately on the different sensing regions, the formation of mycotoxin-BSA-antibody immunocomplexes transfers to electrochemical signal, which would change with the different concentrations of free mycotoxins. Under optimal conditions, the immunosensor array achieved a limit of detection (LOD) of 0.46 pg/mL for AFB1 and 0.34 pg/mL for FB1 within a wide dynamic range from 1 pg/mL to 20 ng/mL. Furthermore, the AFB1 and FB1 spiked in the ground corn and wheat extracts were detected with satisfactory recoveries, demonstrating the excellent practicality of this established method for simultaneous detection of mycotoxins.

Distribution of antioxidants and phenolic compounds in flour milling fractions from hard red winter wheat

Mature wheat kernels contain three main parts: endosperm, bran, and germ. Flour milling results in multiple streams that are chemically different; however, the distribution of antioxidants and phenolic compounds has not been well documented in terms of conventional milling by-product streams. In this study, multiple analytical methods were used to investigate antioxidant activity and phenolic compound compositions of hard red winter wheat (whole ground wheat), the parts of a wheat kernel (bran, flour, germ), and wheat by-product streams (mill feed, red dog, shorts) for the first time. For each mill stream, phenolic compounds (total, flavonoid, and anthocyanin contents) were determined and antioxidant activities were evaluated with 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity, ferric reducing/antioxidant power (FRAP), and total antioxidant capacity assays. Significant differences (P < 0.05) were observed in phenolic concentrations among fractions of bran, flour, and germ milled from the same kernels and noted that germ accounts for the majority of antioxidant properties, whereas bran contains a substantial portion of phenolic compounds and anthocyanins. Mill feed was high in phenolic content (5.29 mg FAE/g), total antioxidant capacity (866 mg/g), and antioxidant activity (up to 75% DPPH inhibition and 20.26 μmol FeSO4/g). The comprehensive information on distribution of antioxidants and phenolic compounds provides insights for future human consumption of commonly produced co-products from flour milling, and for selecting and using different milling fractions to make foods with improved nutritional properties.

Effects of the level and source of dietary physically effective fiber on feed intake, nutrient utilization, heat energy, ruminal fermentation, and milk production by Alpine goats

Thirty-two primiparous and 31 multiparous Alpine goats were used to determine influences of diets varying in level and source of forage on performance in early to mid-lactation for 16 wk. Diets consisted of 40%, 50%, 60%, and 70% forage (designated as 40F, 50F, 60F, and 70F, respectively) with 60F and 70F containing coarsely ground grass hay (primarily orchardgrass) and 40F and 50F containing cottonseed hulls, alfalfa pellets, and coarsely ground wheat hay. Diets contained 15.9% to 16.3% crude protein and 37.8%, 42.1%, 53.5%, and 55.4% neutral detergent fiber (NDF) with 10.0%, 15.8%, 50.1%, and 55.5% particle retention on a 19-mm sieve, and 26.1%, 29.6%, 38.3%, and 40.0% physically effective NDF (peNDF) for 40F, 50F, 60F, and 70F, respectively. Dry matter intake (2.71, 2.75, 1.96, and 1.95 kg/d) and milk yield (2.82, 2.71, 2.23, and 2.10 kg/d for 40F, 50F, 60F, and 70F, respectively) were lower (P < 0.05) for the two diets highest in forage. Digestion of organic matter was similar among diets (P > 0.05), but digestibility of NDF was greater (P < 0.05) for 60F and 70F (57.5%, 58.4%, 68.9%, and 72.2% for 40F, 50F, 60F, and 70F, respectively). Diet affected (P < 0.05) milk fat (3.16%, 3.37%, 2.93%, and 2.97%) and protein concentrations (2.62%, 2.69%, 2.58%, and 2.52% for 40F, 50F, 60F, and 70F, respectively). Milk energy yield was greater (P < 0.05) for the two diets lowest in forage (7.51, 7.45, 5.68, and 5.34 MJ/d), although yield relative to dry matter intake was not affected (P > 0.05) by diet and was lower (P < 0.05) for primiparous vs. multiparous goats (2.71 and 3.09 MJ/kg). Ruminal pH and acetate proportion were greater for 60F and 70F than for the other diets and the proportion of butyrate was lower for the two diets highest in fiber. The mean lengths of time spent ruminating, eating, standing, and lying were not affected (P > 0.05) by diet or parity, but many interactions involving diet, period, hour, and parity were significant (P < 0.05). In conclusion, lactational performance of Alpine goats in early to mid-lactation will be constrained with diets high in forage of moderate quality, peNDF content, and large particle size, which appeared related to limited feed intake.

Effect of Dietary Supplementation with Fish Meal and Soybean Meal on Gastrointestinal Function in Carnivorous Companion Animals – Using Mink (Neovision vision) as a Model

Abstract Soybean meal (SBM) is fed to various livestock species worldwide. It is also present in dog food as a plant protein source, combined with animal-derived ingredients. The aim of this study was to compare the effect of dietary supplementation with fishmeal (FM, an animal protein source) and high amounts of SBM (a plant protein source) on nutrient digestibility, gastrointestinal function and enzyme activity in mink, a model carnivore. The experiment was performed on 14 female American mink of a standard strain. Diet FM was supplemented with 12% FM and 8% ground wheat grain with sugar beet pulp, and diet SBM contained 20% SBM. It was found that the dietary addition of 20% SBM to mink diets negatively affected the digestibility of protein, carbohydrates and energy. Enhanced colonic fermentation processes as a source of additional energy, observed in SBM group mink, were not sufficient to compensate for the loss of nutrients and energy in the upper gastrointestinal tract (GIT). Therefore, SBM should be applied with caution in diets for carnivorous species.

Postbiotics from Saccharomyces cerevisiae fermentation stabilize rumen solids microbiota and promote microbial network interactions and diversity of hub taxa during grain-based subacute ruminal acidosis (SARA) challenges in lactating dairy cows.

High-yielding dairy cows are commonly fed high-grain rations. However, this can cause subacute ruminal acidosis (SARA), a metabolic disorder in dairy cows that is usually accompanied by dysbiosis of the rumen microbiome. Postbiotics that contain functional metabolites provide a competitive niche for influential members of the rumen microbiome, may stabilize and promote their populations, and, therefore, may attenuate the adverse effects of SARA. This study used a total of 32 rumen-cannulated lactating dairy cows, which were randomly assigned into four treatments: no SCFP (control), 14 g/d Original XPC (SCFPa), 19 g/d NutriTek (SCFPb-1X), and 38 g/d NutriTek (SCFPb-2X) (Diamond V, Cedar Rapids, IA) from 4 weeks before until 12 weeks after parturition. Grain-based SARA challenges were conducted during week 5 (SARA1) and week 8 (SARA2) after parturition by replacing 20% dry matter of the base total mixed ration (TMR) with pellets containing 50% ground barley and 50% ground wheat. The DNA of rumen solids digesta was extracted and subjected to V3-V4 16S rRNA gene sequencing. The characteristics of rumen solids microbiota were compared between non-SARA (Pre-SARA1, week 4; Post-SARA1, week 7; and Post-SARA2, weeks 10 and 12) and SARA stages (SARA1/1, SARA1/2, SARA2/1, SARA2/2), as well as among treatments. Both SARA challenges reduced the richness and diversity of the microbiota and the relative abundances of the phylum Fibrobacteres. Supplementation with SCFP promoted the growth of several fibrolytic bacteria, including Lachnospiraceae UCG-009, Treponema, unclassified Lachnospiraceae, and unclassified Ruminococcaceae during the SARA challenges. These challenges also reduced the positive interactions and the numbers of hub taxa in the microbiota. The SCFPb treatment increased positive interactions among microbial members of the solids digesta and the number of hub taxa during the SARA and non-SARA stages. The SCFPb-2X treatment prevented changes in the network characteristics, including the number of components, clustering coefficient, modularity, positive edge percentage, and edge density of the microbiota during SARA challenges. These challenges reduced predicted carbohydrate and nitrogen metabolism in microbiota, whereas SCFP supplementation attenuated those reductions. Supplementation with SCFP, especially the SCFPb-2X attenuated the adverse effects of grain-based SARA on the diversity and predicted functionality of rumen solids microbiota.

Dependence of the uniformity of feed mixing in the feed mixing device of the granulation line on the number of paddle shaft revolutions and mixing time

Today, 87 percent of the number of cattle, as well as 90 percent of the number of sheep and goats in Uzbekistan are cared for by farmers. 85-90 percent of the number of livestock in our Republic belongs to peasant farms, and the preparation of feed for the livestock kept in these peasant farms should be implemented using best practices and modern technologies in developed countries, increasing the competitiveness and economic efficiency of the livestock sector is important. Based on the aforementioned information, a small-sized granulation line and a feed mixing device were created that are comparable to large-sized devices in terms of work quality and meet small livestock and poultry farms in terms of size, productivity, and energy consumption. The homogeneity of the feed mixing ratio in relation to the device’s paddle shaft rotation count and mixing duration was investigated through experimentation. The results of the trials showed that when the feed mixer paddle rotor was turning at a speed of 50 revolutions per minute and the mixing period was set for five minutes, the optimal mixing quality of ground wheat grain, ground maize grain, ground rice, and wheat bran for the manufacturing of granular feed was reached.

Experimental Study on Two-Phase (Solid-Liquid) Flows of Ground Wheat Straw in Inclined Pipes

Highlights Knife milled wheat straw-water slurry flow through inclined pipes at various inclinations is experimentally studied. Slurry concentration and pipe inclination affect the onset velocity of drag reduction and the percentage of drag reduction. The critical mass concentration of maximum drag reduction is a vital function of velocity and is independent of the pipe angle. Downhill wheat straw slurry flows exhibit the most significant reduction in drag and the smallest onset velocities. Wheat straw slurry flows moving uphill exhibit nonmonotonic variations in onset velocity and drag reduction. Abstract. Long-distance pipeline hydro-transport of lignocellulosic biomass for industrial-scale biofuel production at levels comparable to conventional oil refineries presents an economically and logistically viable alternative to fossil fuels. There is very limited understanding on the behavior of the transportation of agricultural biomass slurries in an inclined pipeline. This research is focused on a laboratory-scale investigation of 6.4 mm nominal particle length (d50 = 4.85 mm) knife-milled wheat straw-water suspensions' uphill and downhill flows for a range of pipe inclination and saturated mass concentrations. The range of pipe inclination and saturated mass concentration was -7° to +21° and 5%-30%, respectively. The inclined test section was 29 m long with a 50 mm inside diameter of a closed pipeline loop. The accuracy of the measurements was verified by calibrating the inclined pipe section with fine sand (d50 = 0.103 mm)-aqueous slurries and comparing the results with established correlations. Most wheat straw-aqueous suspensions in the inclined flows showed the characteristics of the plug flow and the transition flow regions together for saturated mass concentration, Cm = 5%-30% and the entire flow range (0.5-4.7 m s-1), with a clear dependence of both the onset velocity of drag reduction (vOD) and drag reduction (%DR) on the pipe inclination as well as the slurry concentration and the critical concentration of maximum drag reduction (Cm)cr on a specific range of suspension velocity. Because of the accelerating effect of gravity, downhill slurry flows had the lowest vOD and the highest %DR at every Cm and pipe inclination with a maximum drag reduction of 25.53% at vm = 4.5 m s-1 and Cm = 25%. Uphill flows demonstrated some nonmonotonic changes in vOD and %DR, which need more experimental data for us to reach a firm conclusion. The research outcomes could help design and operate a long-distance integrated pipeline network for biomass transportation to produce biofuels on a large scale. Keywords: Biomass transport, Drag reduction, Flow regions, Frictional pressure drop, Pipe inclination, Wheat straw slurry.

Production of mycelium-based biomaterial using various substrates

This experiment was carried out to create a biomaterial with mycelium as its main component and binding part. The main object of this research was mycelium of Pleurotus ostreatus. Various substrates were used for mycelium growing: buckwheat husks, onion husks, garlic husks, coconut husk substrate, coffee grounds, birch sawdust, wheat grains, ground wheat, hemp husks, rock wool, and polystyrene. These different substrates and materials were mixed with the mycelium culture and grown for at least three months, with growth monitored weekly. The blocks of grown biomaterial were dried and tested for properties such as water absorption, moisture content, and biodegradation. The conducted studies showed that mycelium can successfully colonise and grow on different substrates, forming biomaterials with different properties. The choice of substrate influenced the physical characteristics of the obtained biomaterials. Mycelium can be used as a sustainable alternative for the production of various biomaterials. The results of this study add to the knowledge in the field of mycelium-based biomaterials and open the way for further research and development of this promising technology.

Microwave-Assisted Digestion Using Dilute Nitric Acid and Hydrogen Peroxide for Multielement Determination in Wheat Flour by ICP-MS

A closed-vessel microwave digestion method was developed for the simultaneous determination of macro, micro, and toxic elements (Mg, K, Ca, Al, Mn, Fe, Cu, Zn, Se, Rb, V, Cd, and As) in wheat by inductively coupled plasma mass spectrometry (ICP-MS). A two-level factorial design with four variables was employed to determine the optimal conditions for the significant parameters. The optimal conditions for microwave digestion of ground wheat samples were found to be 0.5 g of the sample, 9 mL of 8 mol/L HNO3, and 3 mL of 33% H2O2 at 210°C. The analytical method was validated, and the accuracy of the method was then evaluated through analysis of wheat flour-certified standard reference materials (NIST SRM 1567b). The validated procedure was subsequently applied to the analysis of 12 wheat samples sourced from local farms in the Al-Qassim region and a further six samples purchased from the Al-Qassim markets, covering six imported brands common in Saudi Arabia. The results of the study showed that the level of minerals in wheat grown in desert areas is higher than in other areas. The concentrations of macroelements and microelements in the Al-Qassim samples exceeded those of the imported samples by 12.41% and those of the samples from other countries by 33.90%. The results were also evaluated using multivariate analysis techniques: principal component analysis (PCA) and hierarchical cluster analysis (HCA).

Biofortificação do grão de trigo para aminoácidos essenciais

Abstract The objective of this work was to select wheat genotypes aiming to increase the essential amino acids in their grains. The study was carried out in the 2019 crop year, in a randomized complete block design, organized in a 5x5 factorial arrangement – five environments in the state of Rio Grande do Sul, Brazil (Cachoeira do Sul, Cruz Alta, Santo Augusto, São Gabriel, and Vacaria), and five wheat genotypes ('BRS Parrudo', 'Marfim', 'Quartzo', 'TBIO Mestre', and 'TBIO Sinuelo') –, with two replicates. Polar metabolites were extracted from the flour of the ground wheat grains, derivatized, and evaluated by gas chromatography-mass spectrometry. Both variance components and genetic parameters were estimated for the metabolites. To select the genotypes for the traits of interest, the multi-trait index based on factor analysis and ideotype design, the multi-trait genotype-ideotype distance index, and the multi-trait stability index were applied. The wheat genotypes express a high genetic variability and selection possibility for gentiobiose, butyric acid, galactopyranosyl, phenylalanine, tryptophan, leucine, and isoleucine. The 'Marfim' genotype remains stable for essential amino acid levels in the studied environments. The 'Quartzo' genotype stands out in the expression of leucine, isoleucine, phenylalanine, and tryptophan in its grains.

The effect of coupling agent on the mechanical properties of injection molded polypropylene/wheat straw composites

The objective of this work is to evaluate the effect of maleic anhydride-grafted polypropylene (MAPP) as coupling agent in polypropylene (PP)-based composites filled with ground wheat straw (WS) particles. The WS and the MAPP content in the composites was 10 wt% and 2 wt%, respectively. The samples were fabricated through melt compounding using a twin-screw extruder and then formed into dumbbell-shaped specimens by injection molding. The mechanical properties of neat PP and the composites with and without coupling were evaluated based on tensile and flexural tests and dynamic mechanical analyses (DMA). The experimental results showed that incorporating WS into the PP reduces its tensile strength by ~3 MPa, while improving its Young’s modulus by 0.14 GPa. The addition of MAPP compensated for the loss in tensile strength without affecting the modulus. Similar observations were made during the flexural tests as well, in which case, however, there was no loss revealed in the strength in the presence of WS due to the different types of load. The results of DMA analyses indicated an improved stiffness of WS-containing samples throughout the whole analyzed temperature range of 20-120 °C as a consequence of reduced chain mobility of PP caused by the stiff straw particles.

Effects of Protein Source, Whole Wheat and Butyric Acid on Live Performance, Gut Health and Amino Acid Digestibility in Broiler Chickens.

A total of 896 1-day-old straight-run (Ross-308) broilers were used to investigate the interactive effects of protein source (PS), diet structure (DS) and butyric acid (BA) on live performance and carcass characteristics, gut development and its morphology and apparent ileal digestibility (AID) of protein and amino acids (AA). Eight experimental diets comprising 8 replicates with 14 birds each were tested in a 2 × 2 × 2 factorial arrangement with complete randomized design by two levels of BA (0 and 0.1%), two forms of DS (whole vs. ground wheat) and two PS, i.e., soybean meal and canola meal (SBM vs. CM). Throughout the entire experimental period (0 to 35 d), broilers fed SBM-based diets exhibited better (p < 0.05) growth performance (feed intake (FI), body weight gain (BWG) and feed conversion ratio (FCR)), carcass parameters (p < 0.05), gut health (p < 0.05), and nutrient digestibility (p < 0.05) than CM-fed broilers. Dietary whole wheat (WW) positively affected FI (p = 0.001), BWG (p = 0.004) and FCR (p = 0.035) during the overall experimental period. Broilers fed WW had 6, 5, 8, 11 and 10% lower empty relative weights of crop, proventriculus, jejunum, ileum and colon and 25 and 15% heavier gizzard and pancreas, respectively, with longer villus height (p < 0.001), reduced crypt depth (p = 0.031) and longer villus height-to-crypt depth ratio (p < 0.001) than those fed ground-wheat-based diets. Broilers fed WW had greater (p < 0.05) AID of CP and most of the AA. Butyric acid supplementation resulted in improved (p < 0.05) growth performance and digestibility of threonine, valine, leucine, isoleucine, phenylalanine, serine and aspartate. The broilers consuming SBM had 28% lower abdominal fat than those fed CM-based diets. In conclusion, harmful consequences of a less digestible PS can partially be compensated by the inclusion of WW, and supplementation of BA further reduces these detrimental effects.

Ecotoxicological effects of untreated pig manure from diets with or without growth-promoting supplements on Eisenia andrei in subtropical soils.

This study aimed to evaluate the effects of untreated pig manure from diets incorporating growth-promoting supplements (antibiotics and Zn oxide) on the survival and reproduction of Eisenia andrei earthworms. The tested manures were obtained from four different groups of pigs fed with four different diets: CS, a diet based on corn and soymeal; TR, a diet based on corn, soymeal, and ground wheat (15%); CSa, a diet based on corn and soymeal + 100ppm of doxycycline + 50ppm of colistin + 2500ppm of Zn oxide; and TRa, a diet based on corn, soymeal, and ground wheat (15%) + 100ppm of doxycycline + 50ppm of colistin + 2500ppm of Zn oxide. The study used two soils representative of the Southern region of Brazil (Oxisol and Entisol). In general, there were no significant differences between the different manures tested in each soil. However, there were differences in the toxicity manure on E. andreibetween the soils, and the magnitude of this effect was dependent on the applied dose. In Oxisol, LC50 values were higher than 80 m3ha-1, and EC50 varied from 9 to 27 m3ha-1. In Entisol, the LC50 values were below the lowest dose tested (< 25 m3ha-1), and EC50 remained around 5 m3ha-1. It may be possible that the effects observed were attributed to an excess of nitrogen, copper, and zinc, promoted by the addition of the untreated manureand how these factors interacted with soil type.

Mineral Composition of Bread Wheat Cultivars as Influenced by Different Fertilizer Sources and Weed Management Practices

Mineral and vitamin deficiencies are one of the important threats especially in developing and under-developed countries. Wheat grain also contains a number of elements vital to our biological functions, but hazardous to our health in high concentrations. This research was carried out to determine the effects of agronomic practices on the mineral composition of organically grown bread wheat (Triticum aestivum L.) varieties. In terms of all nutrients evaluated, the mineral content of wheat showed significant differences according to crop years, varieties, weed management methods and fertilizer sources. As the average of all factors, the Cu, Fe, Mn, Se, Zn, Cd, Co, Cr, Ni and Pb contents of the ground wheat grain were 3.93, 42.8, 79.6, 0.549, 11.34, 0.012, 0.140, 0.194, 3.71 and 0.269 mg/kg, respectively. According to the wheat varieties, the Kirik was superior in terms of Cu (7.6%), Fe (3.8%), Se (57%), Zn (40.5%), Co (31.1%) and Cr (36.1%), and the Dogu-88 was superior in terms of Mn (5.5%), Cd (1.9%), and Ni (17.0%). The effect of weed management methods on mineral content was variable. According to fertilizer sources, the highest mineral content was obtained from the control plots without fertilizer treatments. The lowest mineral contents were obtained from chemical fertilization, cattle manure and organic fertilizer applications. There was no significant increase in the mineral content of wheat with organic fertilization, however, organic agriculture still preserves its place in terms of healthy food. As a result, it has been determined that the values obtained for all mineral elements were not at a level that pose a risk on the environment, human and animal health according to WHO. In order to identify wheat varieties with higher mineral content, which are beneficial for human health, new research should be done with different organic fertilizer sources with more varieties.

Effect of graded dietary inclusion levels of hybrid rye grain on productive performance, the cost-effectiveness of nutrition and egg quality in laying hens

Abstract The study was designed to investigate productivity parameters, egg quality and the cost effectiveness of feeding laying hens with diets where ground wheat was replaced with varying levels of whole hybrid rye grain cv. Brasetto. A total of 396 Hy-Line Brown hens at 34 weeks of age were allocated to three treatment groups with 66 replicates (cages) of two hens each, and were fed isocaloric and isonitrogenous pelleted diets for 12 weeks. The diet for control group hens (R0) did not contain rye grain. In diets for hens from groups R100 and R200, ground wheat grain was partially replaced with whole rye grain at 100 g/kg and 200 g/kg, respectively. Whole intact rye grains were included in the diets. The cost of diets containing 10% and 20% of whole rye grain was by approximately 1.0% and 1.9% lower, respectively, compared with the control diet that contained no rye grain. During the entire 12-week feeding period, dietary treatments had no effect on laying rate (95.64% to 97.07%), egg weight (62.51 g to 62.74 g) or egg mass output (5.04 kg to 5.12 kg per hen). The inclusion of whole rye grain in layer diets caused a linear increase in feed intake (P&lt;0.047) and tended to linearly increase the feed conversion ratio (FCR, P = 0.078). Both groups of hens fed diets containing whole rye grain were characterized by a linear decrease in the dry matter (DM) content of excreta (P = 0.001). Increased excreta moisture was accompanied by a tendency towards a higher incidence of dirty eggshells in R200 hens (P = 0.068). The inclusion of 100 g/kg of whole rye grain in layer diets decreased the cost of feed per kg of eggs by 1.9%. Dietary treatments had no significant effect on eggshell thickness (0.330 mm–0.345 mm), albumen quality (Haugh units, 83.46–85.84) or yolk color (3.8–4.0). The results of this experiment indicate that pre-pellet inclusion of whole rye (at up to 200 g/kg) in diets with supplemental NSP-degrading enzymes and phytase can be an effective and economically viable alternative to ground wheat in pelleted layer diets.

Efficacy of whole wheat grain in combination with acidified drinking water on growth performance, gizzard development, intestinal morphology, and microbial population of broiler chickens

A 2 × 3 factorial arrangement of treatments was used to study the effects of acidified drinking water (ADW) as 0 or 350 mL acidifier/1,000 L with 3 different wheat forms including ground wheat (GW), whole wheat (WW), or pelleted whole wheat (PWW) on growth performance, gizzard and proventriculus development, intestinal morphology, and microbial population of broiler chickens fed pelleted corn-soybean meal-based diets. GW, WW, and PWW were added at 50, 100, and 150 g/kg of the diet during starter (d 0 to 10), grower (d 10 to 24), and finisher (d 24 to 35) phases, respectively. A total of 300 male broiler chickens at 1-d-old were randomly distributed into six treatments of five replicate pens each. During the starter and grower phases, GW inclusion tended to increase feed intake (P < 0.05), while WW improved feed conversion ratio (FCR) during all experimental phases (P < 0.05). Feed intake during the grower phase decreased and FCR during the starter and grower phases improved in broiler chickens, which received ADW (P < 0.05). An interaction between the wheat form and ADW were observed for the relative weight and muscular thickness of gizzard and pH of gizzard and proventriculus contents (P < 0.05). Inclusion of WW improved the relative weight, length, and width of gizzard and proventriculus and gizzard muscular thickness (P < 0.05). The gizzard weight increased in broiler chickens received ADW, while pH of gizzard contents decreased in those receiving ADW (P < 0.05). Feeding WW increased villus length (VL) and villus area (VA) compared to PWW inclusion, while the ratio of VL to crypt depth (CD) was the greatest in broilers fed WW (P < 0.05). An increase in VL, VA, or VL/CD was observed in broiler chickens, which received ADW (P < 0.05). In microbial population, ADW tended to increase Lactobacillus and decrease Escherichia coli populations (P < 0.05). Lactobacillus population increased in broiler chickens fed WW diet (P < 0.05). In conclusion, WW inclusion in combination with ADW was found to be a suitable promoting of growth performance, gizzard development, intestinal morphology, and microbial population in broiler chickens.

Monitoring of Anaerobic Degradation in Batch Assays Using Fiber Bags

AbstractThe anaerobic batch digestion of freeze‐dried and ground (&lt; 1.5 mm) maize silage, wheat straw, cattle manure, pig manure, and cellulose filter paper was investigated with substrates placed in polyester filter bags. Gas production dynamics of bagged and non‐bagged substrates were compared. By using batch bottles running in parallel with those used for gas measurement, substrates could be sampled over time and various parameters measured for both substrate and bulk liquid. The bags allowed an immediate mass loss into the bulk liquid with some substrates and a generally lower rate of gas production. This method requires refinement but has potential for the study of the dynamics of substrate degradation during anaerobic digestion.