Leafy Vegetables Research Articles

Phytochemical Study and Radical Scavenging Activity of Three Leafy Vegetables Grown in Zitenga Region of Burkina Faso

The aim of this study is to analyze the phytochemical composition and radical scavenging activity of three leafy vegetables from Burkina Faso: <i>Cleome gynandra, Hibiscus sabdariffa and Corchorus olitorius</i>. Samples of these plants were collected in Zitenga region, dried, powdered and extracted by decoction using water and a hydroethanolic solvent. Phytochemical screening was carried out by thin layer chromatography. Bioactive compounds, notably flavonoids and phenolic compounds, were measured respectively by the aluminum trichloride (AlCl<sub>3</sub>) method and the Folin-Ciocalteu method. The radical scavenging activity of the extracts was also investigated using the DPPH method. Phytochemical analyzes revealed the presence of bioactive compounds such as flavonoids, tannins and saponosides in the three species studied. The highest total phenolic and total flavonoid contents were obtained respectively with the hydroethanolic extract of <i>Cleome gynandra</i> (223.54 ± 0.18 mg TEA/g DM) and the aqueous extract of <i>Cleome gynandra</i> (69.09 ± 0.26 mg QE/g DM). The best antioxidant activity was obtained with the hydroethanolic extract of <i>Corchorus olitorius</i> (IC<sub>50</sub>: 31.93 ± 1.95 µg /mL). The results showed that the extracts of <i>Cleome gynandra, Hibiscus sabdariffa and Corchorus olitoriu</i>s all exhibited radical scavenging activity, indicating their potential to reduce oxidative stress. These results suggest that <i>Cleome gynandra, Hibiscus sabdariffa and Corchorus olitorius</i> are rich sources of health-promoting phytochemicals. These food plants from Burkina Faso could therefore be transformed into nutraceuticals to promote health and prevent diseases linked to oxidative stress.

Upwind Moisture Controls on Interannual Variations of Precipitation and Vegetation in China's Drylands

AbstractDryland precipitation depends on upwind and local moisture sources via moisture recycling. How upwind moisture variations affect interannual variations of downwind precipitation and vegetation in China's drylands remains unclear. We used high‐resolution moisture tracking data sets and found terrestrial moisture (93%) was the dominant moisture source for China's drylands, especially from drylands themselves (46%). In most dryland grids, we observed strong correlations between precipitation and upwind moisture sources from 2003 to 2022 (median r = 0.55), with a more significant effect in drier areas. These demonstrated the upwind moisture control on interannual variations of dryland precipitation, in which internal moisture from drylands exceeds the influence of external terrestrial sources. The upwind moisture variations, especially the recycled moisture of drylands, propagate to influence downwind vegetation greenness in precipitation‐sensitive dryland areas. Our findings revealed that upwind moisture variations induced by climate or land‐cover changes have important implications for water and food security in China's drylands.

Stopover by migrant Montagu's Harriers in the Thar Desert is determined by vegetation greenness and grasshopper abundance but not locust outbreaks

Food availability is an important criterion for migrant birds' decision to stay or leave a stopover site. This can vary depending on the conditions at the site. The Thar Desert of the Indian subcontinent is a major stopover area for birds wintering further south in the region. The desert is also prone to stochastic outbreaks of desert locusts that often coincide with the stopover time of migrating birds. Between 2017 and 2022, we tracked nine tagged Montagu's Harriers (Circus pygargus) using PTT and GPS-GSM transmitters to determine if the stopover duration at stopover sites in the desert is influenced by the availability of migratory locusts or resident grasshoppers and the strategies the birds employ, such as, foraging over a wide area or multiple sites to ensure refuelling during stopover. The mean stopover duration during the 2019 peak locust outbreak was 12 days compared to 18 days in other years. Birds did not track locust occurrence deliberately and preferred to stopover at the same sites each year, irrespective of locust outbreaks. The stopover duration at stopover sites appears to be linked to arrival date, vegetation greenness (NDVI) and marginally to grasshopper abundance. We conclude that the stochastic availability of locusts at stopover sites may provide only an opportunistic source of abundant food for migrant harriers and does not seem to affect their stopover duration. Instead, they choose a more stable strategy of relying on resident grasshoppers, less prone to strong fluctuations in abundance and distribution at the stopover sites.

Evaluating the Impact of Diverse Types of Green Vegetables on Snakehead Fish (Channa Striata) Nursery through an Eco-Friendly Aquaponic Approach

Evaluating the Impact of Diverse Types of Green Vegetables on Snakehead Fish (Channa Striata) Nursery through an Eco-Friendly Aquaponic Approach

BIOACCUMULATION OF CU AND CO IN LEAFY AND FRUIT VEGETABLES FROM THE SUBURBS OF MITROVICA, KOSOVO

Contamination of vegetables with heavy metals is one of the basic environmental problems, especially in developing cities, primarily due to the uncontrolled level of pollution caused by industrial growth and the increase in the number of vehicles using petroleum fuels. This paper aims to determine the bioconcentration of heavy metals Cu and Co in leafy and fruit vegetables grown near Kosovska Mitrovica (Republic of Kosovo). Three places near Kosovska Mitrovica (Zvečan, Frashër, and Polski) are grown: parsley, spinach, lettuce, peppers, eggplant, and cucumber. All analyzed crops are from the 2023 harvest. In the same period, an analysis was made of the soil on which the crops were grown. The bioconversion factor (BF)of the selected leafy and fruit vegetables was also determined. The ICP-MS technique was used to determine the concentration of Cu and Co in soil and leafy and fruit vegetables. From the obtained analyzes it was determined that the soil in Frashër contains the highest amount of Cu and Co - 58.4 mg/kg and 11.6 mg/kg respectively. The amount of Cu in the examined dry leafy and fruit vegetables is in the range from 5.38 mg/kg to 22.4 mg/kg, and of Co from 0.27 mg/kg to 2.99 mg/kg. The bioconcentration factor of the studied leafy and fruit vegetables of Cu and Co is in the range of 0.04 to 0.26 and 0.15 to 0.65 respectively. The accumulation of Co in the analyzed vegetables is higher than the accumulation of Cu, due to the higher BF values for Co. The statistical processing of the results (t-test) showed that there is a difference in the concentration of Cu and Co between leafy and fruit vegetables, but there is no statistically significant difference because the obtained p-values are greater than the marginal p-values.

Association mapping analysis (AMA) for morpho-agronomic traits and leaf aromatic compounds using SSR markers in three types of Perilla crop collected from South Korea.

Perilla is a representative leafy vegetable in South Korea. As K-Food (Korean food) is in the spotlight around the world, there is also increasing interest in Western countries in Perilla crop, an annual plant belonging to the Lamiaceae family. To discover comprehensive information, including genetic and phylogenetic relationships among the 80 native Perilla accessions, using three types of data: simple sequence repeat (SSR) marker data, volatiles profile data, and morpho-agronomic data. This study conducted genotypic and phenotypic analyses on 80 Perilla accessions of three types (cultivated var. frutescens, weedy var. frutescens, weedy var. crispa) from South Korea. Five groups (G1-G5) of the 80 Perilla accessions of the three types were differentiated into two different clusters [genotype-based clustering (GTC) and phenotype-based clustering (PTC)] based on an aroma sensory phenotypic test. A total of 314 alleles were confirmed using 55 Perilla SSR primer sets, and genetic variation in the 80 Perilla accessions was evaluated. Among the three statistical analysis methods, principal coordinate analysis (PCoA) and GTC using data of the 55 Perilla SSR markers revealed perfectly consistent results, whereas PTC produced a total of six clusters. The 10 Perilla SSR markers associated with and significantly correlated with both biochemical and morphological characteristics were selected. These findings are expected to provide valuable information for developing global South Korean Perilla cultivars for further studies in Perilla crop breeding programs.

Leaf growth pattern and morphology of Sauropus androgynus (L.) Merr. in tropical lowland

Chikurmanis or katuk (Sauropus androgynus (L.) Merr.) is a traditional leafy vegetable that is consumed widely in Indonesia. The leaf is rich in vitamins, minerals, nutrients, and dietary fiber. The study aimed to investigate the leaf growth pattern and morphological characteristics of the chikurmanis cultivated in the tropical lowland ecosystem. The research was conducted on local farmland in Marga Sakti Sebelat, Bengkulu, Indonesia on December 2023 by survey method. The results showed that the leaf of chikurmanis grew in the daytime and continued to grow at nighttime as indicated in midrib length, leaf width, and foliole number. It is likely that nighttime growth become predominant in chikurmanis. The plant was a type of shrub with complete organs of stem, branch, root, flower, and leaf. The leaf was arranged as a compound leaf with many folioles in opposite positions. Midrib length (M) x leaf width (W) with a zero intercept of linear regression was the most reliable predictor of leaf area with the formula Y = 0.4964MxW (R2 = 0.9677). Keywords: chikurmanis plant; katuk; leaf area estimation; morphological trait; traditional leafy vegetable

Use of different vegetation indices for the evaluation of the kinetics of the cherry tomato (Solanum lycopersicum var. cerasiforme) growth based on multispectral images by UAV

Abstract This study evaluated seven vegetation indices for the monitoring of a cherry tomato crop using an unmanned aerial vehicle with a multispectral camera that measures in the green, red, and near-infrared spectral bands. A photogrammetric flight plan was designed to capture the spectral images every 2 weeks in two agricultural parcels identified as Treatment 1 ( T 1 {T}_{1} ) and Treatment 2 ( T 2 {T}_{2} ). The corresponding orthophotographs were obtained using digital photogrammetry techniques. Subsequently, vegetation indices were calculated for these orthophotographs. The mean and standard deviation of these indices were extracted, and a statistical analysis was performed to compare the vegetation indices and to analyze their behavior over time. Analysis of variance showed that the ratio vegetation index (RVI), green vegetation index (GVI), normalized difference vegetation index (NDVI), infrared percentage vegetation index (IPVI), green normalized difference vegetation index (GNDVI), and optimized soil-adjusted vegetation index (OSAVI) indices showed significant variation (P-value <0.05) over time. No statistically significant differences between the two treatments were found. IPVI, NDVI, and OSAVI showed less variation in pixel values. RVI, GVI, NDVI, IPVI, GNDVI, and OSAVI proved to be valuable tools for monitoring field crops since these indices responded to the crop growth kinetics.

Change trend and attribution analysis of leaf area index in the East African Plateau from 1982 to 2020.

The ecosystems on the East African Plateau are crucial for maintaining the biodiversity, water resource balance, and ecological equilibrium of the African continent. However, the spatiotemporal variations of vegetation and the driving factors remain unclear. We analyzed leaf area index (LAI) change trends in the East African Plateau based on the GIMMS LAI4g dataset and further conducted attribution analysis combining temperature and precipitation data, as well as 10 Dynamic Global Vegetation Models (DGVMs) in TRNEDY v9. The results showed that LAI of the East African Plateau had a modest change trend from 1982 to 1999 (2.5×10-3 m2·m-2·a-1), but significantly increased from 2000 to 2020 (5.2×10-3 m2·m-2·a-1), which was 2.1 times faster than that during 1982-1999. Temperature and precipitation had weak correlations with LAI from 1982 to 1999, but showed significant correlations from 2000 to 2020. The DGVMs demonstrated consistent attribution results, with temperature and precipitation contributing significantly more to the LAI variations from 2000 to 2020 compared to the period from 1982 to 1999. The results highlighted the key role of climate change in driving vegetation greening on the East African Plateau during 2000-2020, which could provide important evidence for ecological conservation and sustainable development strategies in the region.

Pharmacokinetic and Pharmacodynamic Interactions between Food or Herbal Products and Oral Anticoagulants: Evidence Review, Practical Recommendations, and Knowledge Gaps.

Interactions between food and oral anticoagulants (OACs), particularly vitamin K antagonists such as warfarin, are widely recognized and may also be clinically relevant for direct OACs. Pharmacokinetic and pharmacodynamic interactions with food or herbs can lead to anticoagulation potentiation, increased risk of bleeding, or reduced drug efficacy, all compromising patient safety. We conducted a systematic search for randomized controlled trials (RCTs) on PubMed for assessments of interactions between OACs and various ingestants. Since the RCT evidence was slim, we also reviewed prospective longitudinal studies, case series, and case reports to identify possible associations between foods and anticoagulation therapy. We referred to basic or translational studies that shared putative explanations for such interactions, but we failed to identify high-quality evidence in most cases. The limited evidence, small sample size of the studies, conflicting results, and possible heterogeneity in the contents of herbal products prevent a conclusive assessment of these interactions. Existing evidence suggests that (1) cranberry juice consumption (up to 240 mL/d and probably even more) with warfarin is safe; (2) use of green leafy vegetables with a high daily content (more than 250 µg) of vitamin K should be cautioned for patients receiving warfarin, because it may decrease warfarin efficacy. It is also advisable for patients to maintain highly constant intake of green leafy vegetables to ensure stable warfarin effectiveness; (3) ginger, even in small quantities (excluding commercial ginger-flavored beverages, which contain only negligible amounts of ginger), and mango (more than one fruit) can both potentiate warfarin effects; (4) patients taking OACs should avoid St. John's wort due to diminished anticoagulant effect; and (5) consumption of less than 240 mL of grapefruit juice daily is unlikely to interact with OACs. Future longitudinal observational cohort studies and RCTs with larger sample sizes are needed to study specific interactions between food or herbal products and OACs.

Inoculation with Jeotgalicoccus sp. improves nutritional quality and biological value of Eruca sativa by enhancing amino acid and phenolic metabolism and increasing mineral uptake, unsaturated fatty acids, vitamins, and antioxidants.

Plant growth-promoting bacteria (PGPB) are considered a promising tool for triggering the synthesis of bioactive compounds in plants and to produce healthy foods. This study aimed to demonstrate the impact of PGPB on the growth, accumulation of primary and secondary metabolites, biological activities, and nutritional qualities of Eruca sativa (arugula), a key leafy vegetable worldwide. To this end, Jeotgalicoccus sp. (JW0823), was isolated and identified by using partial 16S rDNA-based identification and phylogenetic analysis. The findings revealed that JW0823 significantly boosted plant biomass production by about 45% (P<0.05) and enhanced pigment contents by 47.5% to 83.8%. JW0823-treated plants showed remarkable improvements in their proximate composition and vitamin contents, with vitamin E levels increasing by 161.5%. JW0823 induced the accumulation of bioactive metabolites including antioxidants, vitamins, unsaturated fatty acids, and essential amino acids, thereby improving the nutritional qualities of treated plants. An increase in the amounts of amino acids was recorded, with isoleucine showing the highest increase of 270.2%. This was accompanied by increased activity of the key enzymes involved in amino acid biosynthesis, including glutamine synthase, dihydrodipicolinate synthase, cystathionine γ-synthase, and phenylalanine ammonia-lyase enzymes. Consequently, the total antioxidant and antidiabetic activities of the inoculated plants were enhanced. Additionally, JW0823 improved antimicrobial activity against several pathogenic microorganisms. Overall, the JW0823 treatment is a highly promising method for enhancing the health-promoting properties and biological characteristics of E. sativa, making it a valuable tool for improving the quality of this important leafy vegetable.

Food-based dietary guidelines for optimizing calcium intakes for reproductive-aged women in Ethiopia using local foods.

Increasing dietary calcium intakes of Ethiopian women of reproductive age (WRA) is a public health priority for reducing pre-eclampsia in pregnancy. Using linear programming, we determined whether locally available foods consumed by WRA in nine regions (urban and rural) and two administrative cities of Ethiopia could provide 1000mg/day of dietary calcium, and we identified food-based recommendations (FBRs) to improve dietary calcium adequacy in each region. Results showed that diets providing 1000mg/day of calcium were feasible in eight regions (40%) of the target populations examined. It would, however, require marked changes for most populations (90%), increasing the number of servings per week of several food groups to levels close to those of high consumers in each population. The selected calcium-specific FBRs integrate well into the 2022 Ethiopian Dietary Guidelines, requiring additional messages to consume green leafy vegetables, milk, root crops, or teff (Eragrostis tef) or to consume a higher number of servings of vegetables than currently recommended, depending on the population. In conclusion, these analyses show that a food-based approach can be used to achieve dietary calcium adequacy among WRA in 40% of the populations examined. For the other populations, food-based interventions alone may be inadequate and other interventions are likely needed.

Assessing sustainability in smallholder vegetable farms in Benin Republic: A matrix approach

This study aims to assess the level of sustainability in vegetable-based agrifood production systems in Benin and to propose actions to enhance sustainability, food safety, and year-round production in the vegetable production systems. Semi-structured interviews were conducted with 200 vegetable farmers in contrasting agroecological areas (with areas of extensive production of staples and intensive production of vegetables), using the “Indicateur de Durabilité des Exploitations Agricoles” (IDEA) framework (an on-farm sustainability index). Most of the surveyed vegetable farmers produced a wide range of crops, including leafy vegetables (amaranth, African eggplant, and African basil) and peppers, grown by more than 50% of the farmers. The average scores achieved by the vegetable farms regarding three dimensions of sustainability—ecological, social, and economic—were 35, 41, and 63, respectively, out of a maximum score of 100. All three sustainability dimensions of the vegetable farms were, on average, at a low level and improvements were needed for them to reach an acceptable standard. The vegetable farms located in the south of Benin had, on average, a higher sustainability score than those in the north: around 50% of vegetable farms in the south had a medium score, while the sustainability level of almost 75% of vegetable farms in the north was low. Interventions seeking to improve the sustainability of vegetable farms in Benin should focus on the promotion and adoption of eco-responsible practices that improve on-farm biodiversity, water conservation, and the effective allocation and management of land and labor, to mitigate the environmental impacts of vegetable production.

Discovering the role of fairy ring fungi in accelerating nitrogen cycling to promote plant productivity in grasslands

Soil microorganisms play a key role in the provision of plant-bioavailable nutrients, which is crucial for ecosystem functioning and plant productivity. Fairy rings are widespread features in grasslands accompanied by lush dark-green vegetation bands. This enigmatic feature is caused by increased soil bioavailable nitrogen (N) due to the expansion of fairy ring fungi (FRF). However, little is known about how FRF enhance soil bioavailable N concentrations. Here, we conducted a survey of 35 fairy rings in temperate grasslands to reveal the role of FRF in regulating soil microorganisms and N cycling using amplicon and metagenomic sequencing. The presence of FRF accelerated organic N mineralization via promoting extracellular enzyme (β-1,4-N-acetylglucosaminidase) activity, leading to a 455% increase in ammonium-N. This further stimulated nitrification to enhance nitrate-N concentration by favoring ammonia-oxidizing archaea. Concomitantly, the increased nitrate-N did not promote denitrification or affect the potential risk of N loss. Furthermore, the relative abundance of other saprotrophic and symbiotrophic fungi was significantly reduced by FRF but the changes in these fungi did not affect the activity of extracellular enzymes involved in N mineralization. Our results suggest that FRF can act as ecosystem engineer species shaping fairy rings by driving soil N cycling without the involvement of other microbial functional groups of saprotroph and symbiotroph to boost plant productivity. Thus, due to the stronger N mobilizing ability, FRF show great potential to be exploited as beneficial microorganisms in plant production and sustainable agricultural development.

Diversity of Mycotoxin-Producing Fungi in Leafy and Fruit Vegetables Sold in Port Harcourt Metropolis, Nigeria

Mycotoxins are secondary metabolites produced by fungi and are capable of causing diseases and even death in both humans and other animals. This study investigated the diversity of mycotoxin-producing fungi from leafy (Telfairia occidentalis and Bassica oleracea) and fruit (Solanum lycopersicum and Cucumis sativus) vegetables sold in Port Harcourt metropolis, Nigeria using molecular techniques. The fungi were isolated from vegetable samples showing signs of disease using standard methods. DNA extraction and Polymerase chain reaction were carried out at the molecular laboratory of Regional Centre of Biotechnology and Bioresources research, University of Port Harcourt. The isolates were screened for the presence of mycotoxigenic genes (nor1 (aflatoxin), tri6 (trichothecene), otanps (Ochratoxin A), fum13 (fumonisin) and ZEA (zearalenone)) using PCR technique. The isolates containing mycotoxins were identified using ITS gene sequences. Ten out of the eighteen isolates were positive for four (nor1, tri6, otanps and fum13) out of the five mycotoxigenic genes screened. The nine isolates included fungi belong to six genera: Meyerozyma, Pithomyces, Fusarium, Trametes, Penicillium and Aspergillus. The ten isolates were classified as Meyerozyma carbbica RCBBR_Sf13, Pithomyces chartarum RCBBR_Sf5, Fusarium falciforme RCBBR_Sf10, Trametes duplexa RCBBR_Sf17, Trametes versicolor RCBBR_Sf2a, Trametes duplexa RCBBR_Mf1, Fusarium longifundum RCBBR_Mf4, Penicillium soosanum RCBBR_Mf7, Aspergillus aflatoxiformans RCBBR_Sf9, Fusarium circinatum RCBBR_Sf3 based on their ITS gene sequences. Their ITS gene sequences have been deposited in GenBank under the accession numbers OR816039-OR816047, with the exception of Fusarium circinatum RCBBR_Sf3 which could not accession. This study has demonstrated that mycotoxin-producing fungi are diverse and widespread in leafy and fruit vegetables sold within Port Harcourt Metropolis. This raises both public health and food security concerns.

Assessment of the effects of winter wheat scattering on SAR backscatter for soil moisture estimation based on a radiative transfer model

ABSTRACT This study evaluated the potential of the Single Scattering Radiative Transfer (SSRT) model coupled with the Oh model to retrieve surface soil moisture (SSM) in two drip-irrigated wheat fields in the Tensift al Haouz plain, over three growing seasons (2016–2019). This research focuses on evaluating isotropic scattering and Rayleigh scattering by calibrating the coupled SSRT_Oh model, using data gathered on the calibrated field. The data includes measured SSM at 5 cm depth, Leaf Area Index (LAI) and vegetation height (H). The aim is to fit the extinction coefficient through comparing simulated and Sentinel-1 backscatter, at VV and VH polarizations. The validated field data were used to retrieve SSM. Calibration revealed the best retrieval for Rayleigh scattering, at VV polarization, with an RMSE of 1.25 dB, a correlation coefficient (r) of 0.86, and a bias of 0.1 dB, whereas, the isotropic approach yielded r, RMSE and bias values of 0.83, 1.37 dB and −0.01 dB, respectively. Rayleigh’s performance remained unchanged throughout the inversion process for SSM retrieval, at VV polarization, with r of 0.87 against 0.86 for isotropic scattering. To enhance our findings, the introduction of dynamic extinction parameter and exclusive use of Sentinel-1 data to describe vegetation could improve SSM retrieval.

Zinc oxide nanoparticles reduce cadmium accumulation in hydroponic lettuce (Lactuca sativa L.) by increasing photosynthetic capacity and regulating phenylpropane metabolism

Due to the continuous production of industrial wastes and the excessive use of chemical fertilizers and pesticides, severe cadmium (Cd) pollution in soil has occurred globally. This study investigated the impacts of incorporating zinc oxide nanoparticles (ZnONPs) into hydroponically grown lettuce (Lactuca sativa) under cadmium stress conditions, to seek effective methods to minimize Cd buildup in green leafy vegetables. The results showed that 1 mg/L of Cd significantly inhibited lettuce growth, decreasing in leaves (29 %) and roots (33 %) biomass. However, when lettuce was exposed to 2.5 mg/L ZnONPs under cadmium stress, the growth, chlorophyll content, net photosynthetic rate (Pn), stomatal conductance (Gs), actual photochemical efficiency of PSII (φPSII), and activity of key enzymes in photosynthesis were all significantly enhanced. Furthermore, ZnONPs significantly decreased the accumulation of Cd in lettuce leaves (36 %) and roots (13 %). They altered the subcellular distribution and chemical morphology of Cd in lettuce by modifying the composition of cell walls (such as pectin content) and the levels of phenolic compounds, resulting in a reduction of 27 % in Cd translocation from roots to leaves. RNA sequencing yielded 45.9 × 107 and 53.4 × 107 clean reads from plant leaves and roots in control (T0), Cd (T1), Cd+ZnONPs (T2), and ZnONPs (T3) treatment groups respectively, and 3614 and 1873 differentially expressed genes (DEGs) were identified. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis identified photosynthesis, carbon fixation, and phenylpropanoid metabolism as the main causes of ZnONPs-mediated alleviation of Cd stress in lettuce. Specifically, the DEGs identified included 12 associated with photosystem I, 13 with photosystem II and 23 DEGs with the carbon fixation pathway of photosynthesis. Additionally, DEGs related to phenylalanine ammonia-lyase, caffeoyl CoA 3-O-methyltransferase, peroxidase, 4-coumarate-CoA ligase, hydroxycinnamoyl transferase, and cytochrome P450 proteins were also identified. Therefore, further research is recommended to elucidate the molecular mechanisms by which ZnONPs reduce Cd absorption in lettuce through phenolic acid components in the phenylpropanoid metabolism pathway. Overall, treatments with ZnONPs are recommended to effectively reduce Cd accumulation in the edible portion of lettuce.

Going with the food flow: The contribution of urban agriculture to a growing African city

ABSTRACT Sub-Saharan Africa's rapid urban growth is causing a decline in urban agricultural land, reducing access to healthy local food for citizens. This paper quantitatively and spatially describes the contribution of urban and peri-urban producers to the availability and accessibility of fresh leafy vegetables in the growing city of Dar es Salaam, Tanzania. Based on over 700 surveys with food vendors and consumers, as well as ethnographic research with farmers, we show how a total of 70 per cent of all amaranth consumed in Dar es Salaam was found to be produced within the city. With an average distance travelled from farm to fork of 11 km, leafy vegetables have a strongly localised foodshed. The results show the ongoing significance of urban areas in meeting their own food demands, emphasising the necessity to explicitly integrate food provisioning in the planning of growing African cities.

Biogenic Volatile Organic Compound Emission and Its Response to Land Cover Changes in China During 2001–2020 Using an Improved High‐Precision Vegetation Data Set

AbstractBiogenic volatile organic compounds (BVOCs) are regarded as important precursors for ozone and secondary organic aerosol, mainly from vegetation emissions. In the context of the expanding trend of vegetation greening, the development of high‐precision vegetation data and accurate BVOC emission estimates are essential to develop effective air pollution control measures. In this study, by integrating the multi‐source vegetation cover data, we established a high‐resolution vegetation distribution (HRVD) data set to develop a high spatio‐temporal resolution emission inventory and investigated the impact of different land cover data sets on emission simulation and impact of land cover change on BVOC emissions during 2001–2020. The annual total BVOC emissions in China for 2020 was 15.66 Tg, which were mainly from trees. The emissions simulated by CNLUCC and MODIS data sets were 1.53% and 1.72% higher than those simulated by HRVD data sets, respectively. The spatial distribution of emission differences was consistent with that of land cover differences. The simulated BVOC emissions by the HRVD data set had the best accuracy as they improved the bias between modeling and observation from 69.06% to 65.35% and decreased the underprediction of observations by a factor of 2.13 compared with simulation by MEGAN default vegetation data. The annual BVOC emissions caused by changing vegetation distribution and LAIv (LAI of vegetation covered surfaces) enhanced at a rate of 72.06 Gg yr−1 during 2001–2020. LAIv was the main driver of emission variations. The total OH reactivity of the resulted BVOC emissions increased at a rate of 1.59 s−1 yr−1, with isoprene contributed the most.

A longitudinal study among female college students' eating behaviors and food group intake based on the change in eating habit stages

Objectives This study aimed to longitudinally determine the eating behavior and food group intake of female students to examine the relationship between eating behaviors and intention to improve eating habits (change in eating habits stages) and help health education use the behavior change theory.Methods This one-year longitudinal study included 130 female students from Japanese colleges. The stage at which eating habits and behaviors (skipping breakfast, eating out, instant food intake, and snacking) and intake of food groups rich in protein, calcium, vitamins, minerals, carbohydrates, and fats and oils were assessed. The stages were as follows; pre-contemplation or contemplation (lower group), preparation (middle group), and action or maintenance (upper group). A self-assessment questionnaire was administered to evaluate eating behaviors and food group intake frequencies using a 5-point Likert scale. P-values of <.05 were considered statistically significant.Results A cross-sectional comparison of eating behaviors and food group intake scores demonstrated significant differences between the stages only in snacking behavior. The upper group consumed snacks significantly less frequently than the middle and lower groups. After one year, a longitudinal comparison of eating behaviors and food group intake scores revealed significant differences in the intake of food groups rich in vitamins and minerals (green and yellow vegetables), fats and oils in the pre-contemplation stage, intake of food groups rich in vitamins/minerals (green and yellow vegetables) and frequency of skipping breakfast in the preparation stage, and frequency of eating out and snacking in the action stage. Each stage demonstrated a decreasing trend in food intake and an increasing trend in the frequency of skipping breakfast, eating out, and snacking. The percentages of students whose eating habits stage dropped during the second year were 49.0%, 100%, and 77.8% in the preparation, action, and maintenance stages, respectively. This indicates that there are cases in which readiness reverses in stages with improved eating habits.Conclusion In health education on eating habits, it is vital to understand the changes in eating habit stages for each eating behavior and details of actual eating behaviors and habits. Thus, health education should align to the needs of each individual to support them in transforming and maintaining a higher stage of change in their eating habits.