Green Synthesis Of Silver Nanoparticles Research Articles

Amperometric Detection of Acrylamide in Fried Food using Green-Synthesized Silver Nanoparticles

Acrylamide, a potential toxin and possible carcinogen, is formed in starchy foods cooked at high temperatures and poses a serious food safety concern. This study presents an amperometric sensor using green-synthesized silver nanoparticles (AgNPs) from neem (Azadirachta indica) leaves for the detection of acrylamide (Acr) in fried foods, with a focus on fried plantain chips. The use of AgNPs in conjunction with the specificity of hemoglobin (Hb) provides a rapid and accurate detection mechanism for Acr. Characterization of the AgNPs revealed their crystalline nature and excellent electron transfer properties, which are essential for effective sensor functionality. The sensor responded quickly and was able to accurately identify acrylamide concentrations as low as 5 mM within just 10 s. In addition, it demonstrated high precision, stability, and robust correlation with high-performance liquid chromatography analyzes in real food samples, confirming its practicality as an essential tool for ensuring food safety and quality control. This study highlights the benefits of green synthesis in acrylamide sensing and promotes the use of environmentally friendly materials in food safety applications.

Cytotoxic and Apoptotic Effects of Green Synthesized Silver Nanoparticles via Reactive Oxygen Species-Mediated Mitochondrial Pathway in Human Breast Cancer Cells.

Due to their exceptional physicochemical features, green synthesized silver nanoparticles (AgNPs) have been of considerable interest in cancer treatment. In the present study, for the first time, we aimed to green synthesize AgNPs from Euphorbia retusa and explore their anticancer potential on human breast cancer (MCF-7) cells. First, the green synthesized AgNPs (EU-AgNPs) were well characterized by UV-visible spectroscopy, Fourier transmission infrared (FTIR) spectrum, XRD, scanning and transmission electron microscopy (SEM and TEM), and EDX techniques. The characterization data exhibited that EU-AgNPs were spherical in shape and crystalline in nature with an average size of 17.8 nm. FTIR results established the presence of active metabolites in EU-AgNPs. Second, the anticancer effect of EU-AgNPs was evaluated against MCF-7 cells by MTT and neutral red uptake (NRU) assays. Moreover, morphological changes, ROS production, MMP, and apoptotic marker genes were also studied upon exposure to cytotoxic doses of EU-AgNPs. Our results showed that EU-AgNPs induce cytotoxicity in a concentration-dependent manner, with an IC50 value of 40 μg/mL. Morphological changes in MCF-7 cells exposed to EU-AgNPs also confirm their cytotoxic effects. Increased ROS and decreased MMP levels revealed that EU-AgNPs induced oxidative stress and mitochondrial membrane dysfunction. Moreover, ROS-mediated apoptosis was confirmed by elevated levels of proapoptotic marker genes (p53, Bax, caspase-3, and caspase-9) and reduced levels of an antiapoptotic gene (Bcl-2). Altogether, these findings suggested that EU-AgNPs could induce potential anticancer effects through ROS-mediated apoptosis in MCF-7 cells.

Green synthesis, characterization and biological activity analysis of silver nanoparticles from commercially available root powder of Picrorhiza kurroa Royle ex Benth.

Picrorhiza kurroa Royle ex Benth., a medicinal plant native to the alpine regions of the Himalayas, is renowned for its diverse therapeutic properties. Recently, green-synthesized silver nanoparticles have gained attention for their potential in pharmaceutical and industrial applications. In this study, silver nanoparticles (Pk-AgNPs) were synthesized using commercially available root powder of P. kurroa. The objective was to evaluate their potential biomedical applications by assessing their antioxidant properties and cytotoxicity. The Pk-AgNPs were characterized using Scanning Electron Microscopy (SEM) and Fourier-Transform Infrared Spectroscopy (FT-IR), which revealed their morphology and functional groups. The Total Antioxidant Capacity (TAC) of methanolic root extract of P. kurroa (PkRE) and the Pk-AgNPs was determined using the Phosphomolybdenum method. The results indicated that the antioxidant activity of PkRE was higher than that of the Pk-AgNPs. Moreover, the activity increased with concentration, ranging from 20 µg/mL to 120 µg/mL, demonstrating their capability to eliminate harmful free radicals. The cytotoxicity of PkRE and Pk-AgNPs was evaluated using the MTT assay against the SV40 T-antigen-containing HEK293T cell line, derived from human embryonic kidney 293 cells and the L929 cell line, a mouse fibroblast cell line. The results demonstrated dose-dependent cytotoxicity. L929 cells remained 100 % viable at concentration up to 200 µg/mL for PkRE and 100 µg/mL Pk-AgNPs. In contrast, HEK293T cells didn’t show 100 % viability even at a concentration of 50 µg/mL for both samples. This study highlights the potential use of PkRE and Pk-AgNPs as antioxidants. However, the cytotoxicity findings suggests that they may be harmful to humans if consumed orally, though they could be suitable for use in other organisms. This paves the way for further research into the application of AgNPs synthesized from P. kurroa in biomedical and pharmaceutical domains as well as in veterinary sciences.

Green Synthesis of Silver Nanoparticles and their Potential Applications in Mitigating Cancer.

In recent years, the field of nanotechnology has brought about significant advancements that have transformed the landscape of disease diagnosis, prevention, and treatment, particularly in the realm of medical science. Among the various approaches to nanoparticle synthesis, the green synthesis method has garnered increasing attention. Silver nanoparticles (AgNPs) have emerged as particularly noteworthy nanomaterials within the spectrum of metallic nanoparticles employed for biomedical applications. AgNPs possess several key attributes that make them highly valuable in the biomedical field. They are biocompatible, cost-effective, and environmentally friendly, rendering them suitable for various bioengineering and biomedical applications. Notably, AgNPs have found a prominent role in the domain of cancer diagnosis. Research investigations have provided evidence of AgNPs' anticancer activity, which involves mechanisms such as DNA damage, cell cycle arrest, induction of apoptosis, and the regulation of specific cytokine genes. The synthesis of AgNPs primarily involves the reduction of silver ions by reducing agents. Interestingly, natural products and living organisms have proven to be effective sources for the generation of precursor materials used in AgNP synthesis. This comprehensive review aims to summarize the key aspects of AgNPs, including their characterization, properties, and recent advancements in the field of biogenic AgNP synthesis. Furthermore, the review highlights the potential applications of these nanoparticles in combating cancer.

Green Synthesis of Silver Nanoparticles Using Origanum onites Extract: Effect of Temperature and Time on Antioxidant and Antimicrobial Activity

Green Synthesis of Silver Nanoparticles Using Origanum onites Extract: Effect of Temperature and Time on Antioxidant and Antimicrobial Activity

Unraveling the immune response of the spleen in sepsis using green-synthesized silver nanoparticles from pomegranate peel extracts.

Sepsis is a serious disease characterized by an inappropriate host response to infection, resulting in widespread inflammation and systemic organ failure. The aim of this research is to investigate the possibility of pomegranate peel-derived silver nanoparticles (PGNP) as a potential alternative therapy for sepsis. Characterization using transmission electron microscopy revealed 10-30 nm spherical nanoparticles. In a rat model of sepsis, PGNP treatment improved spleen health, histology, and immune response as compared with septic rats. In rats treated with PGNP during sepsis, significant alterations in oxidative stress markers (p < .01) were observed. These included elevated levels of glutathione (0.63 ± 0.08 mmol/mg protein), reduced concentrations of nitric oxide (8.7 ± 0.8 μ mol/mg protein) and malondialdehyde (2.2 ± 0.3 nmol/mg protein), as well as increased activity of superoxide dismutase (159 ± 33 U/mg protein). Following PGNP administration, gene expression analysis revealed a decrease in spleen IL-1β, IL-6, and TNF-α, highlighting its anti-inflammatory potential. Furthermore, PGNP effectively controlled apoptosis-related genes (Bax, Bcl-2, and Casp3), indicating its role in cellular survival pathways. This study sheds light on the immunological regulation of the spleen during sepsis using PGNP, demonstrating its potential as a new effective treatment approach. The study emphasizes the necessity of continuing to investigate and develop alternative medicines, particularly in light of antibiotic resistance and the global impact of sepsis. RESEARCH HIGHLIGHTS: The study explored the potential medicinal benefits of pomegranate peel-derived silver nanoparticles (PGNP) in the treatment of sepsis. PGNP suppressed pro-inflammatory cytokines and enhanced the immune response. The study recommends PGNP as a viable substitute treatment.

Preparation of hydrogel from the hydroalcoholic root extract of Premna integrifolia L. and its mediated green synthesis of silver nanoparticles for wound healing efficacy

The current investigation concentrated on the fabrication of silver nanoparticles through the root of Premna integrifolia L. The antibacterial and wound healing effects of their silver nanoparticles-hydrogel and root extract-hydrogel were evaluated. The hydroalcoholic root extract of P. integrifolia was enriched with crocetin, ferulic acid, oleanolic acid, oleic acid, syringic acid, and vanillin. The silver nanoparticles were biosynthesized through secondary metabolite-enriched hydroalcoholic root extract (5 %) when mixed with 1 mM AgNO3 and kept under sunlight for 10 minutes. They showed an optimum surface plasmon resonance (SPR) at 447 nm. The aldehyde, phenol, and primary amine groups of the extract reduce silver cations into nanoparticles. The nanoparticles band gap of 2.06 eV showed their semi-conductance behavior. The nanoparticles were spherical, uniformly distributed, and stable. The nanoparticles had a good roughness profile and 57.55 % elemental silver. The silver nanoparticles-hydrogel (10 %) showed an efficient 11±0.11 nm zone of inhibition of S. aureus in comparison to the root extract-hydrogel (10 %), i.e., 9±0.15 nm. The nanoparticles-hydrogel and the root extract-hydrogel did not show noticeable symptoms of acute dermal toxicity. The nanoparticles-hydrogel (10 %) and the root extract-hydrogel (10 %) healed 99.9 % and 97.3 % of the S. aureus- infected wound, respectively. The nanoparticle-hydrogel efficiently induced re-epithelialization in the dermis of S. aureus-infected wound compared to the extract-hydrogel. The nanoparticle-hydrogel enhanced the rate of wound closure.

Green synthesized silver nanoparticles from Ocimum sanctum: A potent inhibitor of biofilm forming ability and efflux pumps in bacteria causing bovine mastitis

Therapeutic management of mastitis faces significant challenges due to multidrug resistance. In the present study, multi-drug-resistant (MDR) Staphylococcus spp, Klebsiella pneumoniae, and Escherichia coli were isolated from bovine clinical mastitis cases and the phenotypic and genotypic multidrug resistance profiling was carried out. Silver nanoparticles (AgNPs) were biosynthesized using Ocimum sanctum leaf extracts and characterized via UV Vis absorption, Fourier Transform Infrared Spectroscopy, X-ray diffraction studies, Energy dispersive spectroscopy and Electron Microscopy. The determined minimum inhibitory concentration and minimum bactericidal concentration of the AgNPs against the recovered MDR isolates were 62.5 μg/ml and 125 μg/ml respectively. At a concentration of 50 μg/ml, the AgNPs demonstrated biofilm inhibitory activities of 80.35 % for MDR E. coli, 71.29 % for S. aureus and 60.18 % for MDR K. pneumoniae. Post-treatment observations revealed notable differences in biofilm formation across bacterial isolates. Furthermore, AgNP treatment led to significant downregulation of expression of the efflux pump genes acrB, acrE, acrF, and emrB in Gram-negative isolates and norB in Staphylococci isolates. This research underscores the potential for the development of an eco-friendly antimicrobial alternative in the form of green synthesized silver nanoparticles to combat drug resistance offering potential antibiofilm and efflux pump inhibitory activities.

Microwave-assisted green synthesis of silver nanoparticles using Mitragyna parvifolia bark extract and their biological activities: an economical and environment-friendly approach

ABSTRACT Nowadays, nanotechnology is extensively employed in the medical profession. In this study, we used the aqueous extract of the bark of Mitragyna parvifolia to synthesize silver nanoparticles (AgNPs) by microwave-assisted green synthesis. The synthesis of AgNPs was confirmed by visual color change to brown color and characteristic surface plasmon resonance peak at 432 nm. The hydrodynamic diameter of the AgNPs was 171.81 nm having a zeta potential of −24.14 mV; Fourier Transmission Infrared Spectroscopy confirmed the presence of different functional groups on the NP surface; Scanning Electron Microscope and High-Resolution Transmission Electron Microscopy indicated predominantly circular shape of nanoparticle; Selected Area Electron Diffraction and X-ray Diffraction analyses determined the crystalline structure of AgNPs. Energy-Dispersive X-ray indicated the elemental composition and formation of AgNPs. The AgNPs were screened at different concentrations for antioxidant activity, antimicrobial, and anticancer potential in breast cancer cells (MCF-7 and MDA-MB-231). The AgNPs exhibited remarkable antioxidant, antimicrobial, and anticancer activities. The sedative and antinociceptive activities were also tested on Swiss albino mice, which showed mild sedative and very potent antinociceptive activity. However, detailed mechanistic studies are warranted in the future for clinical application of the AgNPs as a biologically active agent as well as a carrier for drug delivery.

Green Synthesis of Silver Nanoparticle Using Parmotrema parmutatum Extract and its Application in Colorimetric Sensing and Photolytic Degradation of Organic Dye

Background: Silver nanoparticles possess distinctive characteristics, including chemical stability, high thermal and electrical conductivity, and linear optical properties, making them unique and fascinating. The rise of green synthesis methods for silver nanoparticles is garnering significant interest among researchers, surpassing traditional chemical and physical approaches due to the environmentally friendly, cost-effective, and convenient nature of synthesis. This approach stands as a viable alternative across various sectors, encompassing research, industry, and environmental safety initiatives. Method: Parmotrema permutatum was utilized in the synthesis of silver nanoparticles, followed by their characterization using ultraviolet-visible spectroscopy, Fourier-transformed infrared spectroscopy, X-ray diffraction, energy dispersive X-ray analysis, and scanning electron microscopy. These nanoparticles are subsequiently employed for detecting methylene blue, formaldehyde, and hazardous mercury metal ions as well as photocatalytically degrading methylene blue. objective: Green Synthesis of Silver Nanoparticle Using Parmotrema parmutatum Extract and Its Application in Colorimetric Sensing Results: The silver nanoparticle synthesized was confirmed by a color change and the maximum peak of the SPR band was at 420 nm in the UV spectrum. The crystal has face-centered cubic (FCC) structure with an average size of 12.78 nm. The lichen extract contains polyphenolic groups which act as capping agents. synthesized silver nanoparticles were used to detect formaldehyde and hazardous Hg2+ ions separately. A color change was observed. The detection limit of Hg2+ was 600 μL. Likewise, silver nanoparticles were used to degrade methylene blue. The blue color of methylene blue disappeared. The efficiency of silver nanoparticles was found to be 72% in 4 hours and 87.82% in 24 hours. Conclusion: Parmotrema permutatum has the potential to reduce Ag2+ to Ago and acts as a capping and stabilizing agent, it can be used to biosynthesize silver nanoparticles and can detect formaldehyde, and Hg2+ ions, in addition, it also degrades methylene blue photolytically.

Synthesis of Silver Nanoparticles Using Haplophyllum robustum Bge. Extract: Antibacterial, Antifungal, and Scolicidal activity against Echinococcus granulosus Protoscolices

Background: Silver nanoparticles (AgNPs) biosynthesized via the deployment of plant extractives have garnered much attention, especially due to their antimicrobial properties. Herein, the green synthesis of silver nanoparticles has been accomplished using the aqueous extract of Haplophyllum robustum, which includes a study of its antibacterial, antifungal, and scolicidal activity. Methods: The preparative process was followed by characterization using UV-Vis spectroscopy, and the ensuing spherical AgNPs of average size 7-25 nm were identified by Dynamic Light Scattering (DLS), X-Ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM). The antibacterial, antifungal, and scolicidal activities of AgNPs were assessed by deploying disc diffusion and microdilution methods against four standard bacteria and four typical Candida species and liver hydatid cyst protoscoleces, where they exhibited good biological activity. Results: The results showed that the greener synthesis of silver nanoparticles using the aqueous extract of renewable and abundant H. robustum plant is a simple, inexpensive, and safer alternative that does not use any toxic or harmful substances. Conclusion: Thus, with minimal or no side effects, this approach to AgNPs bodes well for their appliances as antibacterial, antifungal, and scolicidal agents.

Panicum maximum Jacq. mediated green synthesis of silver nanoparticles: synthesis, characterization, and biological activities supported by molecular docking

This study uses the aerial parts of Panicum maximum total extract (PMTE) to synthesize silver nanoparticles (AgNPs) in an environmentally friendly manner. TEM, SEM, FTIR, X-ray powder diffraction (XRD), Zeta potential, UV, and FTIR were used to characterize the green silver nanoparticles (PM-AgNPs). PM-AgNPs were evaluated as anticancer agents compared to (PMTE) against breast (MCF-7), lung (A549), and ovary adenocarcinoma (SKOV3) human tumour cells. The antibacterial activity of AgNPs was assessed against Staphylococcus aureus isolates. The PM-AgNPs had an absorbance of 418 nm, particle size of 15.18 nm, and zeta potential of −22.4 mV, ensuring the nanosilver’s stability. XRD evaluated the crystallography nature of the formed PM-AgNPs. The cytotoxic properties of PM-AgNPs on MCF-7 and SKOV 3 were the strongest, with IC50s of 0.13 ± 0.015 and 3.5 ± 0.5 g/ml, respectively, as compared to A549 (13 ± 3.2 µg/mL). The increase in the apoptotic cells was 97.79 ± 1.61 and 96.6 ± 1.91% for MCF-7 and SKOV3 cell lines, respectively. PM-AgNPs were found to affect the membrane integrity and membrane permeability of 50 and 43.75% of the tested isolates, respectively. Also, PM-AgNPs have recorded a reduction in the biofilm formation of S. aurues. These results suggest using PM-AgNPs to treat breast and ovarian cancers.

Green synthesis of silver nanoparticles using Osbeckia leschenaultiana DC extract: Optimization of synthesis, biological activities, larvicidal activity and toxicity analysis

Presently the Osbeckia leschenaultiana DC leaf - extract was used to synthesis the silver nanoparticles and the green synthesized AgNPs were characterized by UV–vis spectrophotometer, FTIR, XRD, DLS, Zeta potential and SEM. Absorbance at 405 nm was obtained in UV–vis spectrophotometer and multiple peaks emerged in FTIR analyses that represent the presence of some interesting functional groups. XRD results proved that the synthesised AgNPs were face-centered cubic and crystalline in nature. SEM revealed the particles are Cuboid and irregular forms; the particle size ranged between 70 nm and 100 nm. And the antibacterial and antioxidant characterization of green synthesized silver nanoparticles Osbeckia leschenaultiana DC leaf − extract against. Methicillin-resistant Staphylococcus aureus, Vibrio cholerae, Bacillus subtilis and Escherichia coli, revealed the potential antibacterial efficiency against gram positive bacteria Methicillin-resistant Staphylococcus aureus (23.0 ± 1.0) and Bacillus subtilis (22.0 ± 1.0) and they showed 60–70 % high antioxidant activity compared to Osbeckia leaf extract. AgNPs showed the highest mortality against Aedes aegypti with LC50 value of 49.17 µg/mL, followed by the Osbeckia leaf extract have LC50 value of 85.95 µg/mL. The toxicity profile analyzed using non-target organism Artemia salina exhibited less toxicity. The results revealed that the Osbeckia leschenaultiana DC mediated AgNPs had multifaceted antibacterial, antioxidant and larvicidal potential.

Green-synthesized silver nanoparticles from peel extract of pumpkin as a potent radiosensitizer against triple-negative breast cancer (TNBC)

BackgroundTriple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer. Radiation therapy (RT) is a modality for TNBC management. Radiosensitizers can mitigate the adverse effects of RT. Applying green-synthesized silver nanoparticles (Ag-NPs) from biological sources such as plants is a potential strategy to sensitize cancer cells to radiotherapy due to the low toxicity. Therefore, identifying novel natural sources for synthesizing stable and broadly applicable green-Ag-NPs has gained more attention in cancer therapy. In the present study, we synthesized green- Ag-NPs from pumpkin peel extract and elucidated the impact of green-synthesized Ag-NPs as a radiosensitizer in MDA-MB 231 cells (a model of TNBC).ResultsThe prepared Ag-NPs had a spherical shape with an average size of 81 nm and a zeta potential of − 9.96 mV. Combination of green-synthesized Ag-NPs with RT exhibited synergistic anticancer effects with an optimum combination index (CI) of 0.49 in MDA-MB-231 cells. Green-synthesized Ag-NPs synergistically potentiated RT-induced apoptosis in MDA-MB-231 cells compared to the corresponding monotherapies. Morphological features of apoptosis were further confirmed by the DAPI–TUNEL staining assay. HIF-1α expression was decreased in cells subjected to combination therapy. Bax and p53 expression increased, whereas Bcl-2 genes decreased. Combination therapy significantly increased the protein level of PERK and CHOP while decreasing cyclin D1 and p-ERK/total ERK levels compared to monotherapies.ConclusionThese findings indicate the potential effect of green-synthesized Ag-NPs as a radiosensitizer for TNBC treatment.Graphical

GC–MS analysis and green synthesis of silver nanoparticles using Emblica officinalis leaves and evaluation of its antioxidant and in-vitro anti-hypercholesterolemic potential

Currently, various heart conditions, such as hyperlipidaemia, are causing fear among people worldwide. In this research, silver nanoparticles (AgNPs) were produced using Emblica Officinallis leaf extract (EOLE). The synthesis of EOLEAgNPs was examined using UV-visible spectrophotometry at 430 nm, while the shape was determined through SEM analysis. The EDX detected a clear peak of silver (Ag) at 3.4 keV, whereas XRD exhibited a peak distribution at 2 θ° values. The TEM demonstrated a protective layer (100 nm) surrounding EOLEAgNPs. The FTIR (400-4000 cm−1) suggests the phyto-compounds that caused the reduction of AgNO3 to Ag0 ions. The EOLEAgNPs showed a lower value (IC50 = 0.12) and greater antioxidant effects compared to EOLE (IC50 = 4.9). In fact, we discovered that the HMG-CoA reductase enzyme activity of EOLEAgNPs (75.38 ± 0.06) was greater than that of EOLE (37.36 ± 0.03). E. officinallis have a high level of polyphenolics as detected by GCMS-MS. Our research results in the inhibition of HMG-CoA reductase enzyme by EOLEAgNPs, which can effectively treat hyperlipidaemia and promote the development of lipid-lowering biomedicines.

Green Synthesis of Silver Nanoparticles Using Cashew Nutshell Liquid (CNSL): Characterization and Methylene Blue Removal Studies.

In this work, silver nanoparticles (AgNPs) were synthesized from cashew nutshell liquid (CNSL) by varying the concentration of silver ions and the pH of the CNSL extract. The synthesized AgNPs were further characterized to study their surface, structural, and morphological properties and tested for the removal of methylene blue (MB) dye. The results of this study showed that depending on the conditions, particles of various sizes, ranging from 1 to 60 nm, and different degrees of stabilization and agglomeration were produced. The concentration of silver ions equal to 3 mM and the pH of the extract of ~4.5 (AgNP3) resulted in the most efficient synthesis, where particles appeared to be highly stabilized and homogeneously distributed on the surface, exhibiting a small average particle size and a narrow particle size distribution (6.7 ± 6.5 nm). Such particles further showed the highest percent removal of MB, where up to 80% removal was recorded within the first 20 min. Higher concentrations of silver ions and higher pH of the extract resulted in substantial particle agglomeration and particles being over-capped by the CNSL biomolecules, respectively, which further negatively affected the ability of particles to remove MB. Finally, the fact that visible light showed no significant effect on the removal of MB, with the average removal rates found to be about the same as in the dark, suggests the strong catalytic nature of AgNPs, which facilitates the electron transfer reactions leading to MB reduction.

Green synthesized silver nanoparticles based on N-3-(dimethylamino)propyl methacrylamide/2-hydroxyethyl methacrylate hydrogels for antibacterial wound dressing material

In this study, hydrogels were synthesized using Prunus persica ev. Bayramiç Beyazı extract as a crosslinker. N-3-(Dimethylamino)propyl methacrylamide (DMAPMA) and 2-Hydroxyethyl methacrylate (HEMA) monomers were used in hydrogels synthesized by redox polymerization. Hydrogel@Ag composites were created by synthesizing Ag0 nanoparticles within hydrogel network structures cross-linked with plant extract using an in-situ green synthesis method. These hydrogels and composites were characterized by swelling, FTIR, TEM, XRD, TGA methods. The synthesized hydrogels and hydrogel@Ag composites were loaded with Naproxen and Cefazolin drugs. The in-vitro drug release profiles of the hydrogels were examined in a pH = 5.5 and PBS environment, and it was determined that approximately 75% of the drugs were released within 5 h. The release kinetics model for the hydrogels was the Higuchi model, followed by the Korsmeyer-Peppas model. Ag0 nanoparticle encapsulation significantly affected the degree of swelling. The hydrogel cross-linked with MBA showed a swelling capacity of 370.16% by mass, while the hydrogel cross-linked with fruit extract exhibited a swelling capacity of 1000.78% by mass. The silver-encapsulated hydrogel demonstrated an even higher swelling capacity of 2313% by mass. Additionally, antibacterial tests against Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus faecalis microorganisms, showed that Ag-free hydrogels did not exhibit antibacterial activity.

Green Synthesis of Silver Nanoparticle from Anadenanthera colubrina Extract and Its Antimicrobial Action against ESKAPEE Group Bacteria.

Given the urgent need for novel methods to control the spread of multidrug-resistant microorganisms, this study presents a green synthesis approach to produce silver nanoparticles (AgNPs) using the bark extract from Anadenanthera colubrina (Vell.) Brenan var. colubrina. The methodology included obtaining the extract and characterizing the AgNPs, which revealed antimicrobial activity against MDR bacteria. A. colubrina species is valued in indigenous and traditional medicine for its medicinal properties. Herein, it was employed to synthesize AgNPs with effective antibacterial activity (MIC = 19.53-78.12 μM) against clinical isolates from the ESKAPEE group, known for causing high hospitalization costs and mortality rates. Despite its complexity, AgNP synthesis is an affordable method with minimal environmental impacts and risks. Plant-synthesized AgNPs possess unique characteristics that affect their biological activity and cytotoxicity. In this work, A. colubrina bark extract resulted in the synthesis of nanoparticles measuring 75.62 nm in diameter, with a polydispersity index of 0.17 and an average zeta potential of -29 mV, as well as low toxicity for human erythrocytes, with a CC50 value in the range of 961 μM. This synthesis underscores its innovative potential owing to its low toxicity, suggesting applicability across several areas and paving the way for future research.

Green Synthesis of Silver Nanoparticles from Rotheca serrata (L.) Steane &amp; Mabb., its Physicochemical Characterization and Evaluation of its Biological Activities

Dear Editor, I was reading the article which was cited above titled ‘Green synthesis of silver nanoparticles from Rotheca serrata (L.) Steane &amp; Mabb., its physicochemical Characterization and Evaluation of its Biological Activities which was ideal and novel attracted the importance of nanoscience and its utility in the field of medicine1-2. The novel synthesis of nano-based particles has played a pivotal role in the scientific field with green synthesis as a pollution-emerging synthesis which emphasises a cost-effective alternative medicine3 The main purpose of the study focused on green synthesis utilizing silver nanoparticles (Ag NPs) using specific leaf extract of Rotheca serrata (L.) Steane and Mabb belong to the family Verbenacea. The author aimed to characterize these nanoparticles and performed the evaluation study for antibacterial, antioxidant and anti-inflammatory activity and their effects on the seed germination process.

Biosynthesis and in vivo wound healing abilities of Dactyloctenium aegyptium-mediated silver nanoparticles used as hydrogel dressing

Wounds offer a medium for the growth of pathogens and their entry into the body, which necessitates effective wound healing treatments. Herein, we report the green synthesis of silver nanoparticles (AgNPs) using Dactyloctenium aegyptium extract as a capping and reducing agent for wound healing applications. The synthesized nanoparticles were characterized by UV-Vis, FT-IR, SEM, XRD, and in vitro antibacterial activity. Nanoparticles were then incorporated into PVA, Na-alginate, and gelatin-based hydrogel dressings to investigate their in vivo wound healing capability in rats. The color change of the reaction mixture and the surface plasmon resonance (400 nm) confirmed the formation of AgNPs. FT-IR analysis revealed the involvement of plant extract phytochemicals in the capping and stabilization of nanoparticles. The nanoparticles were crystalline in nature, with an average crystallite size of 28.03 nm and exhibited antibacterial activity against S. aureus, P. aeruginosa, K. pneumoniae and E. coli (ZOI 19 ± 0.0, 9 ± 0.0, 13 ± 0.0, and 13 ± 0.0 mm respectively). Furthermore, silver nanoparticle-loaded hydrogels showed accelerated wound healing in rats compared to untreated rats and rats treated with a commercial product. Thus, the developed hydrogel dressing has the potential for clinical application in wound healing and infection treatment.