Green Fluorescence Imaging Research Articles

45 Preliminary results of a phase 1 study of intravenous administration of GL-ONC1 vaccinia virus including green-fluorescent protein real time imaging in patients with advanced cancer

45 Preliminary results of a phase 1 study of intravenous administration of GL-ONC1 vaccinia virus including green-fluorescent protein real time imaging in patients with advanced cancer

The Role of Intraoperative Indocyanine Green Fluorescence Angiography in Pediatric Cardiac Surgery

In surgical reconstructions for congenital heart defects, vessel and anastomotic site patency is critical to success. Indocyanine green fluorescence angiography offers potential for a reliable and rapid method of intraoperative assessment. We sought to determine its feasibility in congenital heart surgery. Patients undergoing the following repairs were eligible for imaging: (1) coronary artery reimplantation, (2) coarctation, (3) palliative shunts, and (4) pulmonary artery reconstruction. Adequate postoperative images were obtained in 18 of 30 (60%) patients. Image adequacy was highest for Blalock-Taussig shunts (100%), coarctation repairs (86%), coronary reimplantations (66%), lowest for the hemi-Fontan (0%), Fontan (40%), and pulmonary artery reconstructions (33%). All adequate images showed vessel or anastomotic site patency, which corresponded to the subsequent postoperative echocardiograms and cineangiograms. There were no adverse events or mortalities. Indocyanine green fluorescence imaging may provide an additional intraoperative imaging modality. Ultimately, the surgical procedure may be assessed and revised, if necessary, prior to leaving the operating room. This potentially will reduce the need for subsequent postoperative interventions, along with their associated morbidity and mortality.

Green fluorescent protein imaging of tumor cells in mice.

The use of green fluorescent protein-expressing cancer cells allows real-time, high-resolution imaging of tumor growth, metastasis, and angiogenesis, both in situ and externally. The author reviews the use of GFP technology for studying human cancers in mouse models.