Green Biosynthesis Of Silver Nanoparticles Research Articles

Green biosynthesis of silver nanoparticles using leaf extract of Ammannia baccifera for evaluating their antioxidant, antibacterial and catalytic potentials

The objective of this work is to create an easy, economical, and environmentally sustainable process for making silver nanoparticles (AB-AgNPs) by employing Ammannia baccifera leaf extract as a provider of reducing agents. A surface plasmon resonance (SPR) peak was observed at 413 nm in the UV–Vis absorption spectrum of AB-AgNPs, and it ensured the formation of nanoparticles. The unique bands in FTIR revealed the participation of phytochemicals with effective functional groups in the reduction and stabilization of AB-AgNPs. SEM revealed almost spherical-shaped AB-AgNPs, while EDX confirmed the presence of metallic silver with a strong signal at 3 eV. TEM further verified the formation of spherically-shaped AB-AgNPs, which ranged in size from 13 to 60 nm. The face-centered cubic (FCC) crystal structure of AB-AgNPs was confirmed by an XRD study. TGA, DLS, and Zeta potential studies also assured the stability and particle size distribution of AB-AgNPs. The DPPH and ABTS radicals were used to investigate the antioxidant activity of plant extract and AB-AgNPs, where the best performance of AB-AgNPs was observed in scavenging the ABTS radical with an EC50 value of 27.52 μg/mL. The green-produced AB-AgNPs showed strong catalytic activity for the breakdown of organic dyes (methylene blue, rhodamine B, and para-nitrophenol), and complete dye degradation was attained for each dye within 20 min. AB-AgNPs also showed efficient activity against Escherichia coli in antibacterial tests. These findings suggest that silver nanoparticles (AB-AgNPs) produced from the extract of Ammannia baccifera leaves, may find use in medicinal and environmental contexts.

Nigella sativa seed-mediated green biosynthesis of silver nanoparticles and antimicrobial activity

This work introduces a systematic and efficient approach for producing stable AgNPs utilizing Nigella sativa (Ns) seed aqua extract (AE), which exhibit strong antibacterial properties. The characterization of Ns-AgNPs was performed using a UV-visible spectrophotometer (UV-Vis), X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, scanning electronic microscopy (SEM), and transmission electronic microscopy (TEM), and energy-dispersive X-ray spectroscopy (EDX). The Ns-AgNPs did not show aggregation, as shown by the results of STEM and XRD. According to the EDX analysis in this research, it was determined that Ns-AgNPs, gave signals in the silver region (~3 KeV) at 92.3%. Ns-AgNPs showed significant antibacterial performance against Staphylococcus aureus, Escherichia coli and were effective at the low concentrations. Ns-AgNPs may be incorporated into wound dressings, surgical instruments, and medical devices to prevent infections and promote healing.

Green biosynthesis of silver nanoparticles from aqueous Zingiber montanum flower extract for enhanced application in medical diagnostic biosensors

Green biosynthesis of silver nanoparticles from aqueous Zingiber montanum flower extract for enhanced application in medical diagnostic biosensors

Green Biosynthesis of Silver Nanoparticles were Obtained from the Extract of Pomegranate (Punica granatum L.) Leaves by Supercritical Extraction Using Microwave Method

In this study, pomegranate (Punica granatum L.) leaf extract and 2% (w/v) aqueous solutions isolated by SFE extraction and microwave extraction were used to create silver nanoparticles (AgNPs). The pomegranate was grown in Turkey's Eastern Black Sea region. AgNO3 solution (0.25, 0.5, and 1 mM) received separate additions of 0.1 and 0.2 mL extract before being microwave-irradiated. Ag nanoparticles made using green chemical techniques were characterized by UV-Visible, , XRD, TEM, Zetasizer and FT-IR. By analyzing the plasmon resonance absorption (SPR) spectra by the UV-Visible technique, the ideal circumstances were identified. The face-centered cubic crystalline silver nanostructures' lattice planes (111), (200), (220), and (311) show that the different Bragg reflection peaks occurred at 2 values of 38.1°, 44.3°, 64.6°, and 77.6°. The average particle size of Ag nanoparticles produced by microwave extraction in an aqueous medium was 86.020.5788 nm, the zeta potential was -140.777 mV, and the polydispersity index was 0.4050.224, according to the results of zeta-Sizer study. The UV-vis absorption spectra of the AuNP solutions, which were kept in a refrigerator, barely altered and remained constant for roughly 4-5 months.

Green Biosynthesis of Silver Nanoparticles from Olive and Walnut-Related Bacteria, Synthesis, Characterization, and Antimicrobial Activity.

The online version contains supplementary material available at 10.1007/s12088-023-01127-z.

Green Synthesis of Silver Nanoparticles by Cardaria draba and Evaluation of its Insecticidal Effect on Schizaphis graminum

Schizaphis graminum one of the main pests of the wheat crop, causing direct and indirect damage to the growth and yield of the crop. The biosynthesis of nanoparticles using plant extracts is the most recent and workable application for pest control. This study was conducted to know the potential activity of insecticidal of synthesized silver nanoparticles (AgNPs) using plant leaf extracts of Cardaria draba. Synthesis was confirmed firstly by visual observation and then by UV-Vis spectrophotometer where The peak was recorded at 428 nm, The results of XRD technique of the colloidal solution confirmed the crystalline nature of the silver nanoparticles. FTIR analysis spectrum showed different types of functional groups which played crucial role in the biosynthesis of AgNPs. FESEM analysis determined the size and shape of AgNPs where average size was 85nm with roughly circular or spherical in shape. Different concentrations of AgNPs were prepared and tested on mortality of the stages of S. graminum, The highest mortality of adults and nymphs recorded 52.99 and 57.13% respectively at the high concentration 100 ppm compared with 4.79 and 5.87% respectively in the control treatment after 3 days from exposure time. In addition to statistically significant differences was in adult and nymph mortality between concentrations. The results suggested that using green biosynthesis of silver nanoparticles can be used as an effective way in the control S. graminum on wheat crops.

Green biosynthesis of stable silver nanoparticles bound with macaranga indica plant extracts for the purification of drinking water

Plant-assisted green biosynthesis of silver nanoparticles (NPs) has become the most powerful technique to prepare stable NPs. Macaranga indica is a medicinal plant widely available in the western ghats. It has verities of medical properties like antibacterial, antioxidant, antidiabetic, cytotoxicity, and antidysentery and is commonly used in the traditional medicine system. This plant also contains polyphenols, flavonoids, and other plant constituents. Here, in this study, macaranga indicia leaf extract is used as a capping agent for the synthesis of silver NPs by using the microwave irradiation technique. Synthesized NPs are characterized by using Uv-Vis Spectra, XRD, EDX, FESEM, and FTIR. Interpretation of characterization data reveals that synthesized NPs are in a spherical shape, monodispersed, and have particles the size of ~15-20 nm. These bicapped silver NPs have shown potential thermotolerant bacterial inhibition activity and are also efficient in methylene blue dye removal and hence can be used in the purification process of drinking water at its source.

Green supported silver nanoparticles over modified reduced graphene oxide: Investigation of its antioxidant and anti-ovarian cancer effects

Abstract A green biosynthesis of silver nanoparticles (AgNPs) decorated Mentha longifolia root extract-functionalized graphene oxide (GO) nanohybrid material has been described. Initially, the Mentha longifolia root was coated on GO’s surface. The phytochemicals of the plant acted as reducing agent for reduction of silver ions and GO to form the rGO-Mentha/Ag nanocomposite. The nanocomposite was characterized using FE-SEM, EDX, FT-IR, TEM, elemental mapping, and XRD analysis. The cells treated with rGO-Mentha/Ag nanocomposite were assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay for 48 h about the cytotoxicity and anti-human ovarian cancer properties on normal (HUVEC) and human ovarian cancer cell lines, i.e., SKOV3 and A2780. The IC50 of rGO-Mentha/Ag nanocomposite were 181.2 and 196.4 µg/mL against SKOV3 and A2780cell lines, respectively. The viability of malignant human ovarian cell line reduced dose-dependently in the presence of rGO-Mentha/Ag nanocomposite. After clinical study, rGO-Mentha/Ag nanocomposite can be introduced as a novel composite in the treatment of human ovarian cancer.

Green Biosynthesis of Silver Nanoparticles from Ocimum gratissimum for Bactericidal Applications

Green Biosynthesis of Silver Nanoparticles from Ocimum gratissimum for Bactericidal Applications

Green synthesis of silver nanoparticles with an antibacterial activity using Salvia officinalis aqueous extract

This report searched the rapid and simple green biosynthesis of silver nanoparticles (AgNPs) using Salvia officinalis aqueous extract. The visual color change from colorless/yellow to reddish brown was noticed indicating the phytosynthesis AgNPs during the process. The presence of functional groups in plant extract acted as reducing and capping agents for the formation of AgNPs was confirmed by FTIR. The fabricated AgNPs were characterized by ultraviolet–visible spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray analysis (EDX). The UV–Visible spectrum showed a characteristic absorption peak for Ag at around 457 nm with optical bandgaps of about 2.22 eV of AgNPs. XRD patterns at (111), (020), (022), (131), and (222) lattice planes depicted the production of face-centered cubic silver nano-structure with approximate crystal size ranges from≈20 nm by Scherrer’s formula. The predominantly spherical nanoparticles having an average size of 20 nm showed enhanced antibacterial activity against Salmonella typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli with more activity toward P. aeruginosa. Our green synthesis protocol required only 15 min for the conversion of silver ions into silver nanoparticles by heating is an efficient, rapid, simple, sustainable, cheap, and energy-efficient, alternative to conventional chemical/physical methods, a one-step application.

Green biosynthesis, characterization of silver nanoparticles using a green alga Spirogyra sp., and their antioxidant and enzyme activities

A simple, environmentally friendly, inexpensive, and one-step alternative method was reported for the green biosynthesis of silver nanoparticles (AgNPs) operating the Spirogyra sp. extract as a reducing and stabilizing substance. Concentration of AgNO3 and reaction time were optimized to prepare AgNPs under controlled conditions. The synthesized silver nanoparticles were characterized by UV-Vis absorption spectroscopy, fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDX), and elemental mapping. The TEM analysis showed that the average particle size of AgNPs was 18.3 nm. Structural details of silver nanoparticles elucidated by Selected Area Electron Diffraction (SAED) based on TEM images. In addition, biological activity tests were applied to nanoparticles and algal extracts to determine antioxidant activity (3 different tests: DPPH (1,1-diphenyl-2-picrylhydrazil) radical scavenging activity, total phenolic content (TPC) and total flavonoid content (TFC)) and α-glucosidase enzyme inhibition. Antioxidant activity and α-glucosidase enzyme inhibition values of silver nanoparticles are higher than the values of Spirogyra sp. extracts.

In vitro and in vivo synergistic wound healing and anti-methicillin-resistant Staphylococcus aureus (MRSA) evaluation of liquorice-decorated silver nanoparticles.

The multi-drug resistant Staph. aureus strain, Methicillin-resistant Staphylococcus aureus (MRSA), is an emerging pathogen that could penetrate skin cuts and wounds, causing a life-threatening condition. The green biosynthesis of silver nanoparticles with liquorice extract has been demonstrated over several years for anticancer and antioxidant effects, as well as antibacterial effect against bothGram-positive and Gram-negative bacteria. The study was designed to evaluate the synergistic in vivo and in vitro wound healing and anti-MRSA activity of decorated liquorice silver nanoparticles (LD-AgNPs). The LD-AgNPs were prepared by thoroughly mixing diluted liquorice extract with AgNO3 at room temperature. The prepared nanoparticles were characterized by size measurement, IR spectroscopy, TEM imaging, and X-ray diffraction. The in vitro and in vivo antibacterial and wound healing testing were also performed. The obtained LD-AgNPs were spherical in shape and had a hydrodynamic size of about 50.16 ± 5.37 nm. Moreover, they showed potent antibacterial activity againstGram-positive and Gram-negative resistant bacteria, produced a significantly higher level of procollagen type I compared to either liquorice extract or standard silver sulfadiazine, and promoted the wound healing process in rabbits. The formulation of silver nanoparticles with liquorice extract showed synergetic effects in enhancing the treatment of wounds, with significant antibacterial activity against E. coli and MRSA.

Green Biosynthesis of Silver Nanoparticles Using Vaccinium oxycoccos (Cranberry) Extract and Evaluation of Their Biomedical Potential

Eco-friendly preparation of metallic nanoparticles (NPs) is a greatly evolving field of scientific research. These types of NPs have gained substantial recognition from scientists, including chemists, chemical biologists and technologists, who have successfully exploited them for the fabrication of a variety of advanced nanodevices. Herein, silver (Ag) NPs were synthesized by a green approach using the aqueous extract of Vaccinium oxycoccos (cranberry), which not only reduced the silver ions but also stabilized the surface of the resultant Ag NPs. The formation of Ag NPs is confirmed by different analytical techniques, including powder X-ray diffraction, UV analysis, scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), transmission electron microscopy (TEM) and Fourier-transform infrared spectroscopy (FT-IR). The UV analysis of the aqueous solution of the reaction mixture demonstrated an absorption band at ~450 nm, which is the typical peak of Ag NPs, leading to the confirmation of product formation. While the XRD confirmed the crystallinity of the sample and the formation of a face-centered cubic (fcc) structure, on the other hand, TEM revealed the presence of spherical NPs with an approximate size range between 5–30 nm. Furthermore, the as-obtained Ag NPs were subjected to thorough investigations to explore the biomedical potential of the sample. In this case, the Ag NPs demonstrated considerable antioxidant and antifungal properties towards various pathogens. In addition, Ag NPs also showed substantial inhibition of spore germination.

Synthesis of biodegradable antimicrobial pH-sensitive silver nanocomposites reliant on chitosan and carrageenan derivatives for 5-fluorouracil drug delivery toward HCT116 cancer cells

The current research relies on a one-pot green biosynthesis of silver nanoparticles (SNPs) with various ratios of silver (Ag) in the existence of N, N, N-trimethyl chitosan chloride (TMC) and carboxymethyl kappa-carrageenan (CMKC), to investigate the effectiveness of the synthesized silver nanocomposites (SNCs) as pH sensitive biodegradable carrier for orally intestinal delivery of 5-fluorouracil (5-FU) drug. FTIR, XRD, TEM and FE-SEM/EDX methods were utilized to demonstrate the structure of the prepared polyelectrolyte complex PEC (TMC/CMKC) and SNCs (TMC/CMKC/Ag). The results showed that the 5-FU encapsulation effectiveness inside all of the prepared SNCs samples was improved by increasing the concentration of Ag, reaching 92.16 ± 0.57 % with 3 % Ag. In vitro release behavior of 5-FU loaded SNC 3 % (TMC/CMKC/Ag 3 %), displayed slow and sustained release reaching 96.3 ± 0.81 % up to 24 h into pH 7.4 medium. The successful release of 5-FU from the loaded SNC 3 % was confirmed through occurrence of strong cytotoxicity, with an IC50 value of 31.15 μg/ml, and high % of apoptotic cells (30.66 %) within the treated HCT116 cells. Besides, SNC 3 % showed good biodegradability and antimicrobial properties against different bacterial strains. Overall, SNC 3 % can be suggested as an effective system for both controlled drug delivery and antibacterial action.

Green Biosynthesis of Silver Nanoparticles Using Annona glabra and Annona squamosa Extracts with Antimicrobial, Anticancer, Apoptosis Potentials, Assisted by In Silico Modeling, and Metabolic Profiling.

Annona glabra L. (AngTE) and Annona squamosa L. (AnsTE) fruits have been widely used in cancer treatment. Accordingly, their extracts were used to synthesize silver nanoparticles via a biogenic route (Ang-AgNPs) and (Ans-AgNPs), respectively. Chemical profiling was established using UPLC-QTOF-MS/MS. All species were tested for anticancer activity against human cervical cancer cells (HeLa), prostate adenocarcinoma metastatic (PC3), and ovary adenocarcinoma (SKOV3) using sulphorhodamine B assay. Apoptosis was determined using Annexin flow cytometry along with cell cycle analysis and supported by a molecular docking. The antibacterial and synergistic effect when combined with gentamicin were evaluated. A total of 114 compounds were tentatively identified, mainly acetogenins and ent-kaurane diterpenes. AnsTE and Ans-AgNPs had the most potent cytotoxicity on HeLa and SKOV3 cells, inducing a significant apoptotic effect against all tumor cells. The AnsTE and Ans-AgNPs significantly arrested PC3, SKOV3, and HeLa cells in the S phase. The nanoparticles demonstrated greater antibacterial and antifungal activities, as well as a synergistic effect with gentamicin against P. aeruginosa and E. coli. Finally, a molecular docking was attempted to investigate the binding mode of the identified compounds in Bcl-2 proteins' receptor, implying that the fruits and their nanoparticles are excellent candidates for treating skin infections in patients with ovarian or prostatic cancer.

Green biosynthesis of silver nanoparticles using sodium alginate extracted from Sargassum latifolium and their antibacterial activity

Brown seaweed Sargassum latifolium produces a sufficient amount of alginate. It is derived from the cell wall of seaweed as a natural anionic polysaccharide. In the present study, alginate was extracted from S. latifolium, its physicochemical properties, the potential for silver nanoparticles formation, characterization of alginate silver nanoparticles, and its antibacterial activity was studied. Physical analysis of extracted sodium alginate as colour (pale yellow), yield (25.34%), water content (13.17%), ash content (15.5%), and pH (8.6) were investigated. In addition, UV scan analyses of extracted alginate illustrated that the bioactive compounds separated in the range of 200–400 nm. On the other hand, ultraviolet–visible spectroscopy (UV–Vis) scan analysis of synthesized alginate silver nanoparticles gave a maximum peak at 413 nm. Besides that, Fourier Transforms Infrared spectroscopy (FT-IR) analysis of alginate, and alginate silver nanoparticles revealed a variety of functional groups, such as –OH, –NH, –CH, –COOH, CO, and C–C, and thermogravimetric analysis of extracted sodium alginate demonstrated thermal degradation occurs in two steps; Dehydration followed by degradation to Na2CO3 and a carbonized substance that breaks down slowly between 600 and 750 °C in N2. Biochemical contents, protein, and carbohydrate were receptively, respectively, and were 0.89 and 78.56 mg/g dry wt. Alginate silver nanoparticles recorded maximal antibacterial activity against Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli, Bacillus subtilis, and Bacillus cereus more than alginate. On the other hand, alginate has no activity against E. coli.

Streptomyces rochei MS-37 as a Novel Marine Actinobacterium for Green Biosynthesis of Silver Nanoparticles and Their Biomedical Applications.

Periodontitis, as one of the most common diseases on a global scale, is a public health concern. Microbial resistance to currently available antimicrobial agents is becoming a growing issue in periodontal treatment. As a result, it is critical to develop effective and environmentally friendly biomedical approaches to overcome such challenges. The investigation of Streptomyces rochei MS-37's performance may be the first of its kind as a novel marine actinobacterium for the green biosynthesis of silver nanoparticles (SNPs) and potentials as antibacterial, anti-inflammatory, antibiofilm, and antioxidant candidates suppressing membrane-associated dental infections. Streptomyces rochei MS-37, a new marine actinobacterial strain, was used in this study for the biosynthesis of silver nanoparticles for various biomedical applications. Surface plasmon resonance spectroscopy showed a peak at 429 nm for the SNPs. The SNPs were spherical, tiny (average 23.2 nm by TEM, 59.4 nm by DLS), very stable (-26 mV), and contained capping agents. The minimum inhibitory concentrations of the SNPs that showed potential antibacterial action ranged from 8 to 128 µg/mL. Periodontal pathogens were used to perform qualitative evaluations of microbial adhesion and bacterial penetration through guided tissue regeneration membranes. The findings suggested that the presence of the SNPs could aid in the suppression of membrane-associated infection. Furthermore, when the anti-inflammatory action of the SNPs was tested using nitric oxide radical scavenging capacity and protein denaturation inhibition, it was discovered that the SNPs were extremely efficient at scavenging nitric oxide free radicals and had a strong anti-denaturation impact. The SNPs were found to be more cytotoxic to CAL27 than to human peripheral blood mononuclear cells (PBMCs), with IC50 values of 81.16 µg/mL in PBMCs and 34.03 µg/mL in CAL27. This study's findings open a new avenue for using marine actinobacteria for silver nanoparticle biosynthesis, which holds great promise for a variety of biomedical applications, in particular periodontal treatment.

Green biosynthesis of silver nanoparticles using Prunus cerasifera pissardii nigra leaf and their antimicrobial activities against some food pathogens

In this study, silver nanoparticles (AgNPs) were synthesised using the Prunus cerasifera pissardii nigra (PC) leaf extract in an easy, low-cost and environmentally friendly way. According to the ultraviolet-visible (UV-vis) spectrophotometer analysis data, the nanocrystals demonstrated a characteristic peak at 456 nm. Scanning electron microscopy (SEM), field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM) and energy-dispersive X-ray (EDX) spectroscopy analyses revealed that the morphological structures of the biosynthesised AgNPs were mostly spherical. According to the results of X-ray diffraction (XRD) analysis, it was determined that the crystal structures of AgNPs were cubic. The size of the nanoparticles was calculated as 23.60 nm using the Debye-Scherrer equation. The zeta potential of the synthesised nanomaterial was measured as –15.5 mV. The minimum inhibitory concentration (MIC) values of AgNPs on Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Bacillus subtilis ATCC 11774, Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATCC 10231 were determined to be 0.062, 0.250, 0.125, 0.500 and 0.125 µg mL<sup>–1</sup>, respectively.

Green Biosynthesis of Silver Nanoparticles from Moringa oleifera Leaves and Its Antimicrobial and Cytotoxicity Activities.

The plant occupied the largest area in the biosynthesis of silver nanoparticles, especially the medicinal plants, and it has shown great potential in biotechnology applications. In this study, green synthesis of silver nanoparticles from Moringa oleifera leaves extract and its antifungal and antitumor activities were investigated. The formation of silver nanoparticles was observed after 1 hour of preparation color changing. The ultraviolet and visible spectrum, Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy techniques were used to characterize synthesis particles. Ultraviolet and visible spectroscopy showed a silver surface plasmon resonance band at 434 nm. Fourier transform infrared analysis shows the possible interactions between silver and bioactive molecules in Moringa oleifera leaves extracts, which may be responsible for the synthesis and stabilization of silver nanoparticles. X-ray diffraction showed that the particles were a semicubic crystal structure and with a size of 38.495 nm. Scanning electron microscopy imaging shows that the atoms are spherical in shape and the average size is 17 nm. The transmission electron microscopy image demonstrated that AgNPs were spherical and semispherical particles with an average of (50–60) nm. The nanoparticles also showed potent antimicrobial activity against pathogenic bacteria and fungi using the well diffusion method. Candida glabrata found that the concentration of 1000 μg/mL exhibited the highest inhibition. As for bacteria, the concentration of 1000 μg/mL appeared to be the inhibition against Staphylococcus aureus. Moringa oleifera AgNPs inhibited human melanoma cells A375 line significant concentration-dependent cytotoxic effects. The powerful bioactivity of the green synthesized silver nanoparticles from medical plants recommends their biomedical use as antimicrobial as well as cytotoxic agents.

Eco-Friendly Green Biosynthesis of Silver Nanoparticles (Or-AgNPs) Using Orange Peel (<i>Citrus sinensis</i>) Waste and Evaluation of their Antibacterial and Cytotoxic Activities

Greener Nanotechnology research depends on the utilization of the reductive potency of a common byproduct of food and fruit processing industry which represent a cheap and reliable source of green reducing agents to be used in bio-nanosynthesis. One potential approach is based on silver bio-synthesis (AgNPs) using biological waste products. Recently, the production of bio-nanoparticles especially AgNPs has received enormous importance due to its good and potential physicochemical characteristics and the possibility of applications .The present work aimed to synthesis silver nanoparticles (AgNPs) using biological orange waste peels products (Citrus Sinensis) to evaluate its characterization, antimicrobial activity and cytotoxicity. The green synthesized silver nanoparticles were characterized by UV-visible spectroscopy and Scanning electron microscopy (SEM). Disk diffusion method was used for the study of antimicrobial activity of the bio-synthesized silver nanoparticles against the different bacterial and fungal strains. Characterization performed by Scanning Electron Microscope and UV visible spectrophotometer where showed the formation of spherical, and few agglomerated AgNPs forms as measured by UV–visible spectrophotometer in the range of 350-550 nm. Antimicrobial activities where showed positive activity against most of the tested human pathogenic bacteria and fungi with varying degrees and the cytotoxicity which performed against African Green Monkey Kidney cell lines (Vero) where CC50 for AgNO3 and AgNPs were ( 76.5±6.27 μl/100μl ) and (9.87±0.90μl/100μl) respectively.