Greater Values Research Articles

Smartphone Technology to Facilitate Remote Postural Balance Assessment in Acute Concussion Management: Pilot Study

Impaired balance is a key symptom following acute concussion. Unfortunately, the recommended clinical balance assessment lacks sensitivity and discriminative ability, relying on the experience of the clinician for interpretation. The aim of this pilot study is to explore smartphone technology to remotely assess balance impairment in people with acute concussion. A smartphone app was developed to allow the clinician to connect remotely using their personal web browser to the participant’s smartphone and collect motion data while instructing the participant to perform the following balance tests: standing on firm and foam surface with eyes opened and closed (FIRMEO, FIRMEC, FOAMEO, and FOAMEC). Outcome measures were processed from the raw acceleration to calculate the average acceleration magnitude from the mean and the root-mean square, with greater values indicating more sway. Eleven healthy controls (HCs) and 11 people with concussion (CON) participated. In all sway measurements, the CON group had significantly (p < 0.05) greater values when standing on a firm surface. In the FOAMEC condition, the CON group had significantly (p < 0.05) greater sway measures only in the AP direction, while significantly greater sway in all directions were found in the CON group in the FOAMEO condition. This study shows that remote balance assessment using a smartphone can discriminate between healthy controls and people with acute concussion. This technology could play an important role in concussion management to assist with determining recovery from concussions and the optimal timing for return to sport.

Chronic acid sphingomyelinase deficiency diagnosed in infancy/childhood in Polish patients: 2024 update.

Acid sphingomyelinase deficiency (ASMD) is an autosomal recessive lysosomal storage disease (LSD) associated with biallelic pathogenic variants in the sphingomyelin phosphodiesterase 1 (SMPD1) gene. The aim of this study was to provide the 2024 update on chronic visceral and neurovisceral ASMD diagnosed in the infancy/childhood in Polish patients. All the patients diagnosed in the pediatric age (0-18 years) with ASMD, both chronic neurovisceral and visceral type, and then systematically followed up, were enrolled into the study. A total number of 7 patients were enrolled into the study. Four patients were previously reported. Two patients were newly recognized with ASMD - 1 with chronic visceral and 1 with chronic neurovisceral ASMD. Splenomegaly was noted in all the patients while a mild liver enlargement was observed in 4 of 7 patients. All patients presented with decreased high-density lipoprotein cholesterol (HDL-C) and decreased serum 25-hydroxy-vitamin D concentration while almost all (6 of 7) with hypercholesterolemia. Cherry-red spot was observed in 5 of 7 patients, including 1 patient with neurovisceral type. Seven various SMPD1 gene variants were identified and missense variants were the most common types of genetic lesions, comprising 71% of all alleles. In all the screened patients, lyso-sphingomyelin (lyso-SM) in dried blood spot (DBS) was found elevated; however, the greater values were observed for patients with chronic neurovisceral type. Chronic acid sphingomyelinase deficiency (ASMD) is a slowly progressive disease. Pediatric ASMD is characterized by spleno-hepatomegaly, dyslipidemia (with decreased HDL-C as the most characteristic) and infiltrative (interstitial) lung disease. Both visceral and neurovisceral chronic ASMD patients could present with cherry-red spot. Both acid spingomyelinase activity and lyso-spingomyelin concentration in DBS should be regarded as a first-tier screening method into ASMD.

Relationship between Repeated Sprint Ability and Force–Velocity Profile in Elite and Subelite Female Field Hockey Players

This study aimed to compare two female field hockey teams of different competitive levels by analyzing kinematic variables in repeated sprint ability (RSA) tests and the force-velocity profile (FVP). Twenty-five female hockey players representing the elite and subelite levels from the same club volunteered to participate. The RSA protocol included six 30 m maximal sprints with a 30 s recovery. Kinematic variables, such as sprint time, step frequency, and step length, were analyzed for each sprint. Additionally, players performed counter-movement jumps (CMJs) and CMJs with 50% body weight (CMJ50s) to calculate the FV50 using the Bosco Index. The elite players showed better (≈2%; p < 0.05) fatigue indexes in sprint time (0–30 m and 0–10 m sections), step length (0–10 m, 10–20 m, and 20–30 m sections), and step frequency (20–30 m section) during the RSA test, as well as greater values (>10%; p < 0.05) in the CMJ50 and FV50 tests. In addition, these RSA (sprint time, step frequency, and step length) and jumping (CMJ, CMJ50, and FV50) variables showed a moderate, significant, or very significant relationship with each other. Therefore, it seems that both strength and speed capacities can be used either in conjunction or as a complementary approach to enhance the overall RSA performance.

Biochemical analysis and fertilization success of Cirrhinus mrigala ova during induced spawning

Fish egg quality is very crucial in aquaculture sector for production of healthy seed. Egg yolk is an energy reservoir for growth and development of embryo. This study evaluated the biochemical composition and quality of Cirrhinus mrigala eggs at three different hatchery sites of Pakistan, (Site 1= Fish Seed Hatchery, District Pakpattan; Site 2= Sidhuwan Hatchery, Head Balloki, District Kasur; Site 3= Chenab Fish Hatchery, Rangpur, District Muzaffargarh) during induced breeding. For this, a total of 36 (18 males and 18 females) fish brooders, 12 (06 males and 06 females) from each site were utilized. Fatty acids analysis revealed significant differences among three different sites. Saturated fatty acids i.e., palmitic acid (C16:0) and stearic acids (C18:0) were higher at site 2 compared to the others sites. Monounsaturated fatty acids i.e., Oleic acid (C18:1) and polyunsaturated fatty acids i.e., Docosahexaenoic acid (DHA) (C22:6) exhibited considerably greater values at site 2 than those of other two sites. Egg mineral contents unveiled remarkable differences, particularly at site 2 indicating significantly higher mineral contents except copper (Cu) in comparison to the other sites. Significant variation exists in fertilization and hatching rates during induced spawning, with the highest values recorded at site 2. It is concluded that biochemical composition of egg especially fatty acid profile and mineral content greatly influences the embryonic development and hatching success of farm reared Cirrhinus mrigala.

Growth Performance and Nutrient Utilization of Balami and Yankasa Rams Fed Leucaena leucocephala and Dried Poultry Manure Based Concentrates Diets

In this work, two feed concentrates—dried poultry manure concentrate (DPMC) and Leucaena leucocephala-based concentrate (LLBC)—fed to rams of two breeds (Yankasa and Balami) were assessed for their proximate composition, digestibility, and nitrogen utilization. In comparison to LLBC, which has more crude protein (15.26%), lower fiber (12.33%), and somewhat higher ether extract (3.21%), DPMC has higher dry matter (90.54%) and fiber (20.55%) but lower crude protein (13.58%) and ether extract (3.84%). Additionally, LLBC exhibits a greater nitrogen-free extract (46.23%) and ash content (8.45%) in comparison to DPMC, which results in a slightly higher total energy content (18.96 MJ/kg against 17.76 MJ/kg). Breed, feed, or breed × feed interaction impacts on performance indicators are not significant (P>0.05). Breed effects (P<0.05) were noted on digestibility, with Balami rams outperforming Yankasa rams in the digestibility of dry matter, crude protein, and nitrogen-free extract. LLBC increased(P<0.05) the digestibility of nitrogen-free extract and dry matter compared to DPMC. The findings indicate that as compared to Yankasa rams fed LLBC, Balami rams fed DPMC showed greater (P<0.05) digestibility of dry matter, crude protein, and nitrogen-free extract. The effects of breed, feed, and breed × feed interaction on nitrogen utilization were significant (P<0.05). Breed has a significant (p<0.05) impact on nitrogen intake, digestion, and retention; Yankasa has greater values than Balami. In comparison to DPMC, LLBC resulted in increased nitrogen intake and retention. Interestingly, the interaction effect reveals that whilst Balami rams retained more nitrogen with DPMC, Yankasa rams retained more nitrogen with LLBC. With implications for optimizing feed strategies based on breed-specific requirements, this study highlights the impact of both feed type and breed on nutritional efficiency.

469 Reducing crude protein levels with amino acid supplementation in the finishing diets of heavy Duroc sire line pigs: Effects on carcass and meat traits in males and females

Abstract Nutrition represents 70% of pig production costs, particularly protein; therefore, decreasing protein costs is of capital importance. Reducing crude protein (CP) contents to levels below recommended whilst supplementing with amino acids (AAs) is an interesting strategy to reduce costs without decreasing production performances. It has been conducted essentially in lean genotypes during the growth stage. Few studies address heavy genotypes or sex differences during the finishing period. Our objective is to study carcass and meat (loin) traits of heavy [Duroc X (Landrace X Large-White)] male and female pigs during the finishing stage under a 2% CP reduction with crystalline AA supplementation, hypothesizing that both sexes will be affected equally. In total, 60 males and 60 females, 9 wk old animals were used and allotted in split-sex pens (6 pigs/pen) with water and dry feed ad libitum. At 15 wk of age, when pigs weighed an average of 68 kg, two isoenergetic diets with different CP levels were provided (145 and 125 g CP/kg, control and low-CP) during the last 42 d of fattening. In the low-CP diet, crystalline AAs were added. On last day of the trial, animals weighed 116.5 kg on average and were slaughtered. Hot carcass weights and yields, ham fat thickness, lean meat percentage and boar taint by human nose scoring were determined. Loin samples (one per pen) were used to determine thawing losses, pH, color, Texture Profile Analysis (TPA: hardness, cohesiveness, springiness) and chemical composition (moisture, ash, protein, collagen, fat, and saturated fat). Data were analyzed using the simple least squares models that included dietary treatment and sex and their interaction as fixed effects with the pen as the experimental unit. Carcass weights were greater in control males than in females (91.2 vs 85.7 kg; P < 0.05). No effect of sex or diet was detected on carcass yields, lean contents or ham subcutaneous fat thickness. There was a great impact of sex for loin subcutaneous fat thickness, with males having 7 to 8% greater values in both dietary treatments (P = 0.011). Low-CP females had a greater percentage of carcasses of greater lean content compared with the three other groups (P < 0.05). Boar taint, carcass pH, color traits, chemical analysis and TPA did not differ across diets. A strong dietary influence was noticeable with animals of both sexes in the Low-CP diets having greater fat and saturated fat contents than animals in the control groups (P = 0.02). In conclusion, the 20g/kg CP reduction supplemented with AAs can efficiently be used in heavy Duroc sire line genotype during the finishing phase without leading to major impairments in carcass or meat traits, regardless of sex.

Non‐Linearity in Mean Annual Peak Flow Scaling With Upstream Basin Area: Insights From Percolation Theory

ABSTRACTUnderstanding how annual peak flow, , relates to upstream basin area, , and their scaling have been one of the challenges in surface hydrology. Although a power‐law scaling relationship (i.e., ) has been widely applied in the literature, it is purely empirical, and due to its empiricism, the interpretation of its exponent, , and its variations from one basin to another is not clear. In the literature, different values of have been reported for various datasets and drainage basins of different areas. Invoking concepts of percolation theory as well as self‐affinity, we derived universal and non‐universal scaling laws to theoretically link to . In the universal scaling, we related the exponent to the fractal dimensionality of percolation, (i.e., ). In the non‐universal scaling, in addition to , the exponent was related to the Hurst exponent, , characterizing the boundaries of the drainage basin (i.e., ). The depends on the dimensionality of the drainage system (e.g., two or three dimensions) and percolation class (e.g., random or invasion percolation). We demonstrated that the theoretical universal and non‐universal bounds were in well agreement with experimental ranges of reported in the literature. More importantly, our theoretical framework revealed that greater values are theoretically expected when basins are more quasi two‐dimensional, while smaller values when basins are mainly quasi three‐dimensional. This is well consistent with the experimental data. We attributed it to the fact that small basins most probably display quasi‐two‐dimensional topography, while large basins quasi‐three‐dimensional topography.

Dynamics of automated body condition scores in grazing Holstein cows in a year-round calving system

This observational study aimed to characterize the seasonal dynamics of automated body condition scores (BCS) through the lactation of Holstein cows in a pasture-based system with year-round calvings. Examining the association between nadir BCS (nBCS; defined as the lowest daily BCS after calving) and peak milk yield within each calving period (calendar seasons equally divided in early and late) was a secondary objective of this research. Retrospective data included 2,164 lactations in 539 primiparous (PRI) and 1,625 multiparous (MLT) Holstein cows that calved from July 2021 to June 2023 in a commercial dairy farm located in Southern Chile. Individual BCS were generated in every milking by an automated BCS camera system. Body condition score at calving (BCSc), nBCS, and the change in BCS from calving to nadir (ΔBCS) were considered for the analysis. Peak milk yield (greatest daily milk yield) values were retrieved from on-farm software and lactations were categorized considering quartiles (Q1 = 25% lowest peak milk yield; Q4 = 25% greatest peak milk yield). Data were examined in PRI and MLT cows separately using multivariable linear models that considered calving period and peak milk yield as the main explanatory variables of interest, while BCSc and health status were included as covariables when appropriate. The differences in BCSc among calving periods were more evident in primiparous cows that had the lowest BCSc in early spring (3.13 ± 0.03) and the greatest BCSc in late fall (3.51 ± 0.03). In MLT cows, the lowest and greatest BCSc were recognized in late fall (3.07 ± 0.04) and early summer (3.23 ± 0.03), respectively. Only MLT cows had differences in nBCS and ΔBCS across calving periods: The lowest nBCS were identified in late spring (2.71 ± 0.02), early summer (2.71 ± 0.02), and in late summer (2.71 ± 0.02). The greatest losses in BCS also occurred in early summer (−0.41 ± 0.02) and in late spring (−0.41 ± 0.02). Time from calving to nBCS varied among calving periods and depended on parity category. Primiparous cows reached nBCS earlier in late summer (65 ± 8 DIM) and late spring (68 ± 7 DIM), while MLT had the shortest times to nBCS in late and early fall (67 ± 5 DIM; 68 ± 5 DIM). Greater values at milk peak were associated with lower nBCS only in multiparous cows during the late spring period. We concluded that the dynamics of BCS were associated with calving period in both parity groups. This data could help guide decisions on nutrition and breeding strategies in farms facing comparable conditions.

Various Features of the X-class White-light Flares in Super Active Region NOAA 13664

Super active region NOAA 13664 produced 12 X-class flares (including the largest one so far, an occulted X8.7 flare, in solar cycle 25) during 2024 May 8–15, and 11 of them are identified as white-light flares. Here we present various features of these X-class white-light flares observed by the White-light Solar Telescope (WST) on board the Advanced Space-based Solar Observatory and the Helioseismic and Magnetic Imager (HMI) on board the Solar Dynamics Observatory. It is found that both the white-light emissions at WST 3600 Å (Balmer continuum) and HMI 6173 Å (Paschen continuum) show up in different regions of the sunspot group in these flares, including outside the sunspots and within the penumbra and umbra of the sunspots. They exhibit a point-, ribbon-, loop-, or ejecta-like shape, which can come from flare ribbons (or footpoints), flare loops, and plasma ejecta depending on the perspective view. The white-light duration and relative enhancement are measured and both parameters for 3600 Å emission have greater values than those for 6173 Å emission. It is also found that these white-light emissions are cospatial well with the hard X-ray (HXR) sources in the on-disk flares but have some offsets with the HXR emissions in the off-limb flares. In addition, it is interesting that the 3600 and 6173 Å emissions show different correlations with the peak HXR fluxes, with the former one more sensitive to the HXR emission. All these greatly help us understand the white-light flares of a large magnitude from a super active region on the Sun and also provide important insights into superflares on Sun-like stars.

Extrapolation is not the same as interpolation

We propose a new machine learning formulation designed specifically for extrapolation. The textbook way to apply machine learning to drug design is to learn a univariate function that when a drug (structure) is input, the function outputs a real number (the activity): f(drug) →\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\rightarrow$$\\end{document} activity. However, experience in real-world drug design suggests that this formulation of the drug design problem is not quite correct. Specifically, what one is really interested in is extrapolation: predicting the activity of new drugs with higher activity than any existing ones. Our new formulation for extrapolation is based on learning a bivariate function that predicts the difference in activities of two drugs F(drug1, drug2) →\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\rightarrow$$\\end{document} difference in activity, followed by the use of ranking algorithms. This formulation is general and agnostic, suitable for finding samples with target values beyond the target value range of the training set. We applied the formulation to work with support vector machines , random forests , and Gradient Boosting Machines . We compared the formulation with standard regression on thousands of drug design datasets, gene expression datasets and material property datasets. The test set extrapolation metric was the identification of examples with greater values than the training set, and top-performing examples (within the top 10% of the whole dataset). On this metric our pairwise formulation vastly outperformed standard regression. Its proposed variations also showed a consistent outperformance. Its application in the stock selection problem further confirmed the advantage of this pairwise formulation.

Analytical and fine Monte Carlo Kerma coefficients evaluation for a wide range photon energy relevant to some chemotherapy agents

Simultaneously combination of chemotherapy drugs and ionizing radiations has become a common treatment method for advanced cancers. Tumor irradiation in presence of chemotherapy drugs can affect deposited energy within tissue. Hence, to evaluate how chemotherapy drugs affect the absorbed dose within tissues during chemoradiotherapy, Kerma coefficient values for 26 anti-cancer agents were estimated for photon energies ranging from 1 keV to 20 MeV through analytical analysis and Monte Carlo (MC) simulation approaches.Estimation of Kerma coefficients through MC simulation relevant to different chemotherapy drugs was performed with MCNPX V.2.6.0 MC code. Besides, Kerma coefficient values were calculated using the reported mass energy absorption coefficients, as the theoretical method. Significant statistical test was also employed to evaluate the accordance of obtained Kerma coefficients by two considered methods.Simulation results were consistent with evaluated Kerma coefficients by analytical method which minimum and maximum mean relative differences are found for Bevacizumab and Methotrexate, respectively. Furthermore, a significant correlation is found with chemical composition of selected chemotherapy drugs at considered photon energy range. Fluorouracil showed the closest Kerma ratio to unity among the studied anti-cancer drugs. Kerma coefficient ratios for most of selected drugs were close to unity at higher energy regions (100 keV–20 MeV) while greater values were found at lower photon energies (below 100 keV).From the results, presence of these anti-cancer agents can impact Kerma coefficient and subsequently alter the absorbed dose within tissue during the cancer treatment with chemoradiotherapy technique.

Successful application of photocatalytic recycled TiO2-GO membranes for the removal of trace organic compounds from tertiary effluent

Photocatalytic membranes are a promising technology for water and wastewater treatment. Towards circular economy, extending the lifetime of reverse osmosis (RO) membranes for as long as possible is extremely important, due to the great amount of RO modules discarded every year around the world. Therefore, in the present study, photocatalytic membranes made of recycled post-lifespan RO membrane (polyamide thin-film composite), TiO2 nanoparticles and graphene oxide are used in the treatment tertiary-treated domestic wastewater to remove trace organic compounds (TrOCs). The inclusion of dopamine throughout the surface modification process enhanced the stability of the membranes to be used as long as 10 months of operation. We investigated TrOCs removal by the membrane itself and in combination with UV-C and visible light by LED. The best results were obtained with integrated membrane UV-C system at pH 9, with considerable reductions of diclofenac (92%) and antipyrine (87%). Changes in effluent pH demonstrated an improvement in the attenuation of TrOCs concentration at higher pHs. By modifying membranes with nanocomposites, an increase in membrane hydrophilicity (4° contact angle reduction) was demonstrated. The effect of the lamp position on the light fluence that reaches the membrane was assessed, and greater values were found in the middle of the membrane, providing parameters for process optimization (0.29 ± 0.10 mW cm−2 at the center of the membrane and 0.07 ± 0.03 mW cm−2 at the right and left extremities). Photocatalytic recycled TiO2-GO membranes have shown great performance to remove TrOCs and extend membrane lifespan, as sustainable technology to treat wastewater.

Evaluation of long-term results of single intraoperative electrical neurostimulation after autologous plastic surgery of a resection defect of the tibial portion of the sciatic nerve in adult rats

Introduction World literature data indicate the effectiveness of single intraoperative electrical stimulation (IES) of the proximal segment of the damaged nerve to stimulate its regeneration, but there is no data on its effect on the long-term results of autoplasty of resection defects.The purpose of the work was to evaluate the long-term results of a single IES after autologous plastic surgery of the tibial portion of the sciatic nerve in rats.Materials and methods Thirty rats after autologous repair of the resection defect of the tibial portion of the sciatic nerve were divided into series 1 (unstimulated control, n = 16) and series 2 (single IES for 40 minutes, n = 14). At 4 and 6 months after surgery, the static sciatic functional index (SFI) and morphometry of epoxy transverse semithin sections of the tibial nerve at the level of the middle third of the leg were assessed. For comparison with the normal values, the corresponding data from 7 intact rats were used.Results The number of animals with excellent results of SFI restoration was 12.5 % in series 1 and 50 % in series 2 (p = 0.05). The numerical density of regenerated myelinated fibers (MF) exceeded the norm: in series 1 — by 63 % (p < 0.01) and 34 % (p < 0.01), in series 2 — by 58 % (p < 0.01) and 47 % (p < 0.01), respectively. In series 2, there were greater values in comparison with series 1: the median diameters of MFs were by 11.7 % and 15.7 %, the median diameters of their axons were by 5.4 % and 11.9 %, the median thickness of the myelin sheath was by 17.0 % and 24.1 %, respectively (p < 0.05 4 months and p < 0.01 6 months after surgery). Four months after surgery in series 1 and 2, the numerical densities of endoneurial vessels exceeded the intact control by 134 % (p < 0.05) and 156 % ( 0.05), their average diameters by 18 % and 16 % (p < 0.01) respectively, and lumen diameters increased only in series 2 by 8 % (p = 0.07). After 6 months of the experiment in series 1 and 2, the numerical densities of microvessels decreased, but significantly exceeded the control by 66 % (p < 0.05) and 83 % (p < 0.05), the average diameters — by 14 % and 36 % (p < 0.05), lumen diameters — by 26 % (p < 0.05) and 50 % (p < 0.01), respectively.Discussion The difference between stimulated and unstimulated animals in all MF size parameters 6 months after surgery was greater than after 4 months, indicating a persistent neuroregenerative effect.Conclusions A significant increase in the diameters of regenerating nerve fibers in the tibial nerve, as well as the diameters of their axons and the thickness of the myelinated sheaths 4 and 6 months after autoplasty of the tibial portion of the sciatic nerve in the group of animals with a single 40-minute IES of the proximal portion of the sciatic nerve indicates the promoting effect of the applied additive effect on regenerative axono- and myelinogenesis. Increase in the lumens and improvement of blood flow of the endoneurial vessels of the tibial nerve in the series with IES ensured the stability of the neuroregenerative effect. The functional significance of the effects of a single IES is confirmed by a significantly higher percentage of animals with excellent results in restoring the static functional index.

Dynamic contrast enhanced ultrasound in differential diagnosis of hepatocellular carcinoma: A systematic review and meta-analysis.

Non-invasive differential diagnosis between hepatocellular carcinoma (HCC) and other liver cancer (i.e. cholangiocarcinoma or metastasis) is highly challenging and definitive diagnosis still relies on histological exam. The patterns of enhancement and wash-out of liver nodules can be used to stratify the risk of malignancy only in cirrhotic patients and HCC frequently shows atypical features. Dynamic contrast-enhanced ultrasound (DCEUS) with standardized software could help to overcome these obstacles, providing functional and quantitative parameters and potentially improving accuracy in the evaluation of tumor perfusion. To explore clinical evidence regarding the application of DCEUS in the differential diagnosis of liver nodules. A comprehensive literature search of clinical studies was performed to identify the parameters of DCEUS that could relate to histological diagnosis. In accordance with the study protocol, a qualitative and quantitative analysis of the evidence was planned. Rise time was significantly higher in HCC patients with a standardized mean difference (SMD) of 0.83 (95%CI: 0.48-1.18). Similarly, other statistically significant parameters were mean transit time local with a SMD of 0.73 (95%CI: 0.20-1.27), peak enhancement with a SMD of 0.37 (95%CI: 0.03-0.70), area wash-in area under the curve with a SMD of 0.47 (95%CI: 0.13-0.81), wash-out area under the curve with a SMD of 0.55 (95%CI: 0.21-0.89) and wash-in and wash-out area under the curve with SMD of 0.51 (95%CI: 0.17-0.85). SMD resulted not significant in fall time and wash-in rate, but the latter presented a trend towards greater values in HCC compared to intrahepatic cholangiocarcinoma. DCEUS could improve non-invasive diagnosis of HCC, leading to less liver biopsy and early treatment. This quantitative analysis needs to be applied on larger cohorts to confirm these preliminary results.

Distribution of catheterisation laboratories in Indonesia 2017–2022: a nationwide survey

BackgroundGeographical terrains of Indonesia pose a major hindrance to transportation. The difficulty of transportation affects the provision of acute time-dependent therapy such as percutaneous coronary intervention (PCI). Also, Indonesia’s aging population would have a significant impact on the prevalence of acute coronary syndrome in the next decade. Therefore, the analysis and enhancement of cardiovascular care are crucial. The catheterisation laboratory performs PCI procedures. In the current study, we mapped the number and distribution of catheterisation laboratories in Indonesia. MethodsA direct survey was used to collect data related to catheterisation laboratory locations in July 2022. The population data was sourced from the Ministry of Home Affairs. The recent growth of catheterisation laboratories was examined and evaluated based on geographical areas. The main instruments for comparing regions and changes throughout time are the ratio of catheterisation laboratories per 100,000 population and the Gini index (a measure of economic and healthcare inequality. Gini index ranges from 0 to 1, with greater values indicating more significant levels of inequality). Regression analysis was carried out to see how the number of catheterisation laboratories was affected by health demand (prevalence) and economic capacity (Gross Domestic Regional Product [GDRP] per Capita). FindingsThe number of catheterisation laboratories in Indonesia significantly increased from 181 to 310 during 2017-2022, with 44 of the 119 new labs built in an area that did not have one. Java has the most catheterisation laboratories (208, 67%). The catheterisation laboratory ratio in the provinces of Indonesia ranges from 0·0 in West Papua and Maluku to 4·46 in Jakarta; the median is 1·09. (IQR 0·71–1·18). The distribution remains a problem, as shown by the high catheterisation laboratory Gini index (0·48). Regression shows that distribution of catheterisation laboratories was significantly affected by GDRP and the prevalence of heart disease. InterpretationThe number of catheterisation laboratories in Indonesia has increased significantly recently, however, maldistribution remains a concern. To improve Indonesia's cardiovascular emergency services, future development of catheterisation laboratories must be better planned considering the facility's accessibility and density. FundingIndonesia Endowment Fund for Education – Lembaga Pengelola Dana Pendidikan (LPDP)

Impact of Aging and Disuse on Markers of Endoplasmic Reticulum Stress and the Unfolded Protein Response

Aging and disuse can impair skeletal muscle responses to stimuli and are key drivers in dysregulated proteostasis. Such dysfunction can lead to an accumulation of misfolded proteins resulting in endoplasmic reticulum stress (ERS) and the unfolded protein response (UPR). While the UPR blunts global translation and enhances the production of chaperones and foldases, little is known about ERS responses across the lifespan and in disuse contexts. Therefore, the purposes of this study were to examine if: i) the expression of ERS and UPR effector proteins differ across the lifespan in rats, and ii) age-associated patterns were recapitulated by 10 days of hindlimb casting in a separate cohort of rats. We hypothesized that aging would enhance the expression of ERS related proteins, and this expression pattern would be mimicked by 10 days of limb disuse. Male Fischer 344 rats were sacrificed at 3, 6, 12, 18, and 24 months (mo) of age, and plantaris (PLT) and soleus (SOL) muscles were collected for analysis. Six mo female Wistar rats were sacrificed after either 10 days of hindlimb casting or continued ambulation (age matched control), after which whole gastrocnemius muscles were collected for analysis. The effector eukaryotic initiation factor 2 alpha (eIF2a) was different across the lifespan (phospho/pan) in PLT and SOL muscles (p<0.001) and was upregulated in the PLT at 24 mo as compared to 3 mo (P=0.006). The downstream UPR effector C/EBP homologous protein (CHOP) was differentially expressed across the lifespan in PLT and SOL (p<0.001). CHOP was upregulated ~2.79 and 3.08-fold at 18 and 24 mo respectively in the SOL (p<0.001), and 2.62 and 2.44-fold at 18 and 24 mo respectively in the PLT. Finally, principal effector Activating Transcription Factor 6 (ATF6) was differentially expressed across the lifespan (cleaved/pan) in the PLT and SOL (P≤0.024) whereby a 1.23-fold up-regulation was evident at 18 mo in the SOL (P=0.035) and 1.34-fold at 18 mo in the PLT (P=0.026). The CHOP and ATF6 proteins were similarly dysregulated in our disuse model, whereby 1.83-fold (p<0.001) and 1.30-fold (P=0.002) greater values were observed in hind limb casted versus control rats. Effectors of the UPR pathway are upregulated in skeletal muscle across the lifespan in rats, and this effect is recapitulated with disuse. These findings warrant further research for potential therapeutics related to age- and disuse-related skeletal muscle atrophy. Funding for this study was provided by discretionary laboratory funds from MDR. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Enrichment of thawed boar spermatozoa with an intact membrane using Magnetic Activated Cell Sorting

Not all boar sperm samples survive cryopreservation well. A method of eliminating damaged sperm might enable more cryopreserved boar semen to be used for pig breeding. In this study we investigated the use of Magnetic Activated Cell sorting (MACS) to eliminate damaged sperm from thawed boar semen samples. The thawed samples were mixed with Dead cell removal particles and were applied to the column in a SuperMACS II. Different fractions were collected: Original sample (O), Flow-through (FT), and Eluate (E). Sperm membrane integrity, mitochondrial membrane potential and reactive oxygen species were evaluated by flow cytometry after staining with SYBR 14 and propidium iodide, or 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethylbenzimidazolylcarbocyanine iodide, or hydroethidine and dichlorodihydrofluorescein diacetate, respectively. The FT samples had increased membrane integrity, a greater proportion of sperm with high mitochondrial membrane potential and a greater proportion of sperm negative for hydrogen peroxide than O samples (P<0.0001), which in turn had increased membrane integrity than E samples (P <0.0001). However, differences were seen between boars. The FT samples had increased values of live, superoxide positive sperm than O samples (P <0.0001) and O samples had greater values than E samples (P <0.0001), while there was no effect of boar. Sperm quality was best in the FT fraction, comprising approximately 32% of the sperm sample. In conclusion, although there were differences between boars, MACS separation can improve sperm quality in thawed semen samples. It would be interesting to see if this improvement is reflected in fertility outcomes.

Evaluating methods for measuring the leaf area index of encroaching shrubs in grasslands: From leaves to optical methods, 3-D scanning, and airborne observation

Leaf area index (LAI) is a key variable describing ecosystem structure and influencing the exchange of carbon, water, and energy. LAI is often evaluated with indirect methods. However, the accuracy of indirect measurements can vary with canopy structure and is not always generalizable across ecosystems. Previous research has characterized the accuracy of indirect methods for woody plants in forest ecosystems, but it is not well established for woody plants in open ecosystems—despite having large differences in canopy structure. In this study, we compared direct LAI measurements in clonal grassland shrub canopies to three indirect methods: a ceptometer, a handheld 3D scanner (processed using EXScanPro-3.6 software), and NEON's LAI product obtained from airborne hyperspectral imaging (derived from SAVI). To our knowledge, this is the first study to assess the accuracy of leaf area data in woody plants using a handheld 3D scanner and one of few studies assessing the accuracy of the National Ecological Observation Network's (NEON) Airborne Observation Platform—our source of airborne hyperspectral imaging. Data were collected in tallgrass prairie undergoing woody encroachment and three treatments: no herbivore disturbance, bison present, and simulated browsing. We found that direct LAI measurements of control and grazed C. drummondii canopies averaged ∼8.0. The ceptometer accurately estimated LAI in non-browsed canopies but overestimated LAI of browsed canopies by 38 %. One-sided leaf area of ramets measured with a handheld 3D scanner was strongly related to direct measurements (r2=0.86), but underestimated leaf area at greater values. LAI estimated from airborne spectral data underestimated LAI by 55 %. We conclude that a ceptometer was adequate for measuring LAI in dense shrub canopies when browsing was not present, the handheld 3D scanner was adequate for measuring leaf area of individual ramets, and the airborne spectral data was not suitable for estimating LAI of dense, grassland shrub canopies.

A comparative finite element analysis of titanium, poly-ether-etherketone, and zirconia abutment on stress distribution around maxillary anterior implants

Background: The aim of this study was to investigate the influence of abutment material, alveolar bone density, and occlusal forces on stress distribution around maxillary anterior implants. Materials and Methods: An in-vitro study was conducted. The maxillary anterior implant was modeled using a three-dimensional finite element model in D2 and D3 bones with three different abutment materials: titanium, zirconia, and poly-ether-ether ketone (PEEK). Von Mises stress was evaluated after the application of vertical and oblique loads of 100 N, 175 N, and 250 N. Statistical analysis was done by Friedman-Wilcoxon signed-rank test, Mann-Whitney U test, and Kruskal-Wallis test. The probability value &lt;0.05 is considered a significant level. Results: Stress distribution around D3 bone was higher than D2 bone in all the abutment materials with greater values seen in oblique load than vertical load with insignificant difference ( P &gt; 0.05). Statistically insignificant stress values were seen greater in PEEK than titanium or zirconia abutment ( P &gt; 0.05). A statistically significant difference was observed between 100 N and 175 N of load ( P &lt; 0.05). Conclusion: PEEK, zirconia, and titanium as abutment material in the anterior region showed similar properties. The stress on the bone was proportionately increased during the vertical and oblique loads suggesting the influence of mechanical load in crestal bone loss rather than the type of abutment material.

Effects of Official Chess Competition on Salivary Cortisol and Mood Swings in Adolescent Girls: A Win-Loss Approach.

The current study examined the effects of official chess competition on salivary cortisol and mood swings in adolescent girls. Fourteen girl competitive chess players participated in the 5-day Swiss chess tournament held in nine heavy and light rounds. The tournament was performed at 9:00 a.m. (first, third, fifth, seventh, and ninth rounds) and 3:00 p.m. (second, fourth, sixth, and eighth rounds). Salivary cortisol and mood was measured before the tournament, before and after the second, fourth, sixth, and eighth rounds, and following the tournament (10 samples). The resting levels of salivary cortisol had considerably greater values on the first, second, third, and fourth competition days compared to 1 week before the competition (P = 0.001). The post-competition cortisol concentration was significantly higher on the second and third days than before the competition (P = 0.001). Winners had considerably higher levels of salivary cortisol compared to losers (P = 0.001). There was a significant increase in total mode disturbance (P = 0.001), anger (P = 0.009), and tension (P = 0.045) following heavy rounds (second and third day) compared to the values before the competition. At the same time, the Scores of vigor decreased significantly (P = 0.001). The findings of the present study showed participating in the official chess competition increased salivary cortisol and caused negative alterations in mood components associated with the difficulty and outcome of the match, indicating the psychological stress. Hence, psychological interventions can be used for psychological recovery of competitive chess players after the competition.