Great Lakes Region Research Articles

Active and passive sampling methods for grubs of the Asiatic garden beetle, Maladera formosae (Coleoptera: Scarabaeidae), in a corn-soybean rotation

Beginning in the mid-2000s, grubs of the Asiatic garden beetle, Maladera formosae (Brenske) (syn. M. castanea [Arrow]), emerged as significant early-season pests of field corn grown in sandy soils of the Great Lakes region. Overwintered grubs move towards the soil surface in springtime to resume feeding on roots, causing stand losses of up to 40%. Scouting for M. formosae is problematic as the grubs are subterranean, and the adults are nocturnal. The objective of this study was to evaluate sampling methods currently in use for other annual white grub species specifically for use with M. formosae grubs in corn production systems. In a series of two experiments, we evaluated the ability of the compact cutter, golf hole cup cutter, and wire-mesh bait station to detect M. formosae grubs and sense varying population densities. The cup cutter, which takes a deeper but smaller soil volume sample than the compact cutter, sampled more grubs per soil volume and was more sensitive to smaller grub populations. The bait station, a passive sampling technique, and cup cutter were both successful at detecting M. formosae before planting. However, the cup cutter is more adequate and economical since it can be used with less equipment in a single trip to the field. Identification of a standardized sampling methods will enable researchers to predict future infestations and ultimately lead to the development of economic threshold levels for M. formosae in row crop agroecosystems.

Good Intentions Gone Bad? The Dodd-Frank Act and Conflict in Africa’s Great Lakes Region

The Dodd-Frank Act imposes reporting requirements on US companies regarding supply chain links to conflict minerals. Previous research uses within–Democratic Republic of the Congo (DRC) variation in the location of mineral mines to identify the act’s effect on conflict. Due to the presence of spillovers, these previous studies may underestimate the effect. Moreover, the act regulates reporting on minerals mined in the DRC and all surrounding countries. To fully evaluate this legislation, I estimate the effect on the prevalence of conflict events in the DRC and all covered countries. Difference-in-differences estimates suggest that the unintended consequences of this legislation within the DRC are larger than previously reported and that there is no evidence of any effect within all covered countries pooled together. Supplemental analyses (i) investigate possible mechanisms that drive these results and (ii) find that suspending enforcement of the legislation is unlikely to reduce conflict in the DRC.

Quantifying long-term phenological patterns of aerial insectivores roosting in the Great Lakes region using weather surveillance radar.

Organisms have been shifting their timing of life history events (phenology) in response to changes in the emergence of resources induced by climate change. Yet understanding these patterns at large scales and across long time series is often challenging. Here we used the US weather surveillance radar network to collect data on the timing of communal swallow and martin roosts and evaluate the scale of phenological shifts and its potential association with temperature. The discrete morning departures of these aggregated aerial insectivores from ground-based roosting locations are detected by radars around sunrise. For the first time, we applied a machine learning algorithm to automatically detect and track these large-scale behaviors. We used 21 years of data from 12 weather surveillance radar stations in the Great Lakes region to quantify the phenology in roosting behavior of aerial insectivores at three spatial levels: local roost cluster, radar station, and across the Great Lakes region. We show that their peak roosting activity timing has advanced by 2.26 days per decade at the regional scale. Similar signals of advancement were found at the station scale, but not at the local roost cluster scale. Air temperature trends in the Great Lakes region during the active roosting period were predictive of later stages of roosting phenology trends (75% and 90% passage dates). Our study represents one of the longest-term broad-scale phenology examinations of avian aerial insectivore species responding to environmental change and provides a stepping stone for examining potential phenological mismatches across trophic levels at broad spatial scales.

Feeding Strategies for Adapting Lake Sturgeon (Acipenser fulvescens) Larvae to Formulated Diets at Early Life Stages.

Cost-effective feeding management is required to support conservation hatcheries for lake sturgeon (Acipenser fulvescens), an ecologically important species in the Great Lakes region. This study investigated an approach to transition lake sturgeon larvae from live feed (Artemia) to formulated feed and its effect on growth performance, survival, and response to acute hypoxia stress. The first experiment showed that sturgeon had similar (p > 0.05) growth and survival when fed Artemia or the combined feeding of Artemia with the commercial diet (crude protein, 551 g/kg diet). Feeding solely on the commercial or lab-made (crude protein, 491 g/kg diet) diet significantly reduced growth and survival (p < 0.05). In the second experiment, the growth performance of sturgeon (14 days post-hatch, DPH) fed with either Artemia only or combined feeding different feeding durations of two, three, and four weeks followed by a complete transition to the commercial diet. At the end of six weeks, the 3- and 4-week combined feeding periods resulted in significantly higher body weight and survival compared to the 2-week combined and the Artemia only feeding treatments. In the last experiment, sturgeons (27 DPH) were fed only with Artemia or combined feeding of Artemia with the commercial diet for four weeks followed by the complete transition to the commercial diet for two weeks. Eighteen fish from each treatment were investigated the response to acute hypoxic conditions (gradual decrease in dissolved oxygen level from 8 to 2.3 mg/L at the rate of 1 mg/L per hour). When the dissolved oxygen was between 3 and 4 mg/L, the mortality rate of the combination-fed sturgeon (11.7%) was significantly lower than those fed only Artemia (83.3%). These results clearly demonstrate that a commercial diet can partially replace Artemia at early life stages to improve growth, survival, and hypoxia tolerance and thus its co-feeding with Artemia is recommended.

Harvest Mortality of Mallards (Anas platyrhynchos) Banded at Lake St. Clair, CAN and Western Lake Erie, USA

Coastal marshes of Lake St. Clair, in southwest Ontario, CAN and Lake Erie, in northwest Ohio, USA are important migratory staging locations for waterfowl despite experiencing substantial wetland losses. Waterfowl habitat management at these locations focuses on providing food resources for Mallards (Anas platyrhynchos), a highly sought game species. We examined direct recovery rates of Mallards banded prior to the 2014, 2015, and 2016 hunting seasons as a measure of harvest mortality. We compared harvest mortality between the two locations, among years, among four sex and age cohorts, and as a function of body condition. We found that banding location for juvenile Mallards was an important variable predicting harvest mortality. The probability of being a direct recovery was 126% greater for juvenile females and 70% greater for juvenile males banded at Lake St. Clair compared to those banded in northwest Ohio. Average body condition was slightly less for juvenile male Mallards banded in northwest Ohio during 2016 compared to those banded in 2015 or those banded in LSC in 2014 or 2015. We did not detect any other significant differences related to body condition and location or location-year with respect to direct recovery rates. We hypothesize that harvest mortality of juvenile Mallards could be related to differences in harvest regulations, bird abundances, or hunter density between the two study sites, all of which may have different implications for those interested in managing Mallard populations in the lower Great Lakes region.

Impacts of Nile tilapia cage culture on water and bottom sediment quality: The ability of an eutrophic lake to absorb and dilute perturbations

AbstractEnvironmentally sustainable aquaculture depends on accurate understanding of the impacts of aquaculture‐derived organic matter (AOM) and the ability of aquaculture systems to absorb and dilute perturbations. To this end, the present study assessed the impacts of AOM from cage culture of Nile tilapia on the ecology of Lake Victoria, Kenya, using fish cages near Anyanga Beach in Siaya County from December 2018 to October 2019. Four locations were surveyed for organic loadings from cage cultures, located 0, 50, 150 and 500 m (as a control site) away from the cages. The cage aquaculture produced increased P and N concentrations near the cages and a decreased N:P molar ratio. These changes stimulated algal growth which, in turn, affected the water quality. The organic material accumulated on the bottom under the cages, increasing the benthic BOD (BOD, >10 mg/g), a sensitive indicator of the ecological footprint of the cage aquaculture. Further, the negative ORP observed in the benthic layer suggested anoxic bacterial metabolism, possibly causing build‐up of sulphides and methane. These changes altered the abundance and composition of both limnetic and benthic communities. At the beginning of the study, 22 zoobenthic taxa existed around the cages and 18 at the reference sites. Only 3 saprophilous taxa, chiefly gastropods (Physellaspp.), bivalves (Sphaeriumspp.) and oligochaetes (Tubifexspp.) were present at the cage site and 17 at the reference site at the end of the culture period. The Shannon diversity index exhibited a declining tendency with the length of culture period at the cage site, signifying a negative impact of aquaculture on biodiversity. The water quality recovery after cage disturbance is rapid (<4 months), noting there was no significant difference in the water quality recorded at the cage site and the other sampling sites after a fallow period of 4 months. However, the recovery of the sediment and meiofauna was far from complete at the end of this period. Moving the cages slightly (50–100 m) away from the former location may allow the benthic communities to recover and alleviate the problem. Further, the fallowing period, particularly for the Anyanga Beach site, should be extended from four to at least 5 months to allow for the environment to recover. With the rapid increase of cage fish farming in the African Great Lakes Region and with the potential for its occurrence in other lakes, there is a need to develop regulations to guide the industry, as well as the need for continuous monitoring of the environment, in order to provide information to guide investments and ensure sustainable cage farming.

Community capacity and climate change in the Laurentian Great Lakes Region: the importance of social, human, and political capital for community responses to climate-driven disturbances

The Laurentian Great Lakes region in North America is experiencing climate-driven disturbances that threaten the public safety of the region and is forcing communities to respond. Communities vary in their ability to respond to these disturbances based on their existing capacities and access to resources, but responses in the region are uneven and create vulnerabilities to disasters. A virtual workshop was conducted to understand the community responses to climate-driven disturbances in the Great Lakes and identify the essential capacities for effective responses. Results show that the region as a whole has not responded adequately, and although the resources exist to respond, they are not adequately organized, and inequalities between urban and rural communities can exacerbate these challenges. Community capacities identified as critical for resilience include leadership, scientific knowledge, and connection to broader regional networks to access additional resources, but there are challenges with the complacency of some communities and deficiencies in mental health services.

Integrating invasive species risk assessment into environmental DNA metabarcoding reference libraries.

Environmental DNA (eDNA) metabarcoding has shown promise as a tool for estimating biodiversity and early detection of invasive species. In aquatic systems, advantages of this method include the ability to concurrently monitor biodiversity and detect incipient invasions simply through the collection and analysis of water samples. However, depending on the molecular markers chosen for a given study, reference libraries containing target sequences from present species may limit the usefulness of eDNA metabarcoding. To explore the extent of this issue and how it may be resolved to aid biodiversity and invasive species early detection goals, we focus on fishes in the well-studied Laurentian Great Lakes region. First, we provide a synthesis of species currently known from the region and of non-indigenous species identified as threats by international, national, regional, and introduction pathway-specific fish risk assessments. With these species lists, we then evaluate 23 primer pairs commonly used in fish eDNA metabarcoding with available databases of sequence coverage and species specificity. Finally, we identify established and potentially invasive non-indigenous fish that should be prioritized for genetic sequencing to ensure robust eDNA metabarcoding for the region. Our results should increase confidence in using eDNA metabarcoding for fisheries conservation and management in the Great Lakes region and help prioritize reference sequencing efforts. The ultimate utility of eDNA metabarcoding approaches will come when conservation management of existing fish communities is integrated with early detection efforts for invasive species surveillance to assess total fish biodiversity.

Introduction to Special issue

Introduction to Special issue

Review of maternal death audits in refugee camps in UNHCR East and Horn of Africa and Great Lakes Region, 2017-2019.

To review the quality of maternal death audits and identify factors contributing to refugee maternal deaths in the East and Horn of Africa. Maternal death audits submitted to The UN Refugee Agency (UNHCR) from 2017 to 2019 in 43 refugee camps in eight countries were analyzed for completeness, obstetric history, cause of death, and contributing factors. A total of 191 refugee maternal death audits were retrieved. The mean age of the deceased was 28 years (range, 15-45 years), and 13% were adolescents and 17% were of advanced maternal age. Most patients (55%) were grand multigravida (≥5 pregnancies). The majority (86%) attended antenatal care visits, with 51% attending four or more visits. Among women who delivered (n= 140), 91% were facility-based deliveries. Most (68%) deaths occurred postpartum. Obstetric hemorrhage (49%) was the leading direct cause of death (with 77 cases of postpartum hemorrhage), followed by hypertensive disorder (19%) and infection (15%). Delays in care were identified in 185 (97%) cases. Delays in receiving care were more prevalent (81%) than in seeking (61%) and reaching (26%) care. Factors contributing to delays in receiving care highlight the capacity gaps in provision of emergency obstetric care, including management of postpartum hemorrhage, requiring urgent additional investments. Audit findings also show the need for attention and action towards family planning, contraception, and adolescent sexual and reproductive health services.

Phylogeography and genetic variation in Western Jacob's ladder (Polemonium occidentale) provide insights into the origin and conservation of rare species in the Great Lakes region.

The perennial herb Western Jacob's Ladder (Polemonium occidentale, Polemoniaceae) is widespread in the mountains of western North America but reappears as a disjunct in the Great Lakes Region in Minnesota and Wisconsin, USA as the narrow endemic P. occidentale subsp. lacustre. This distribution is shown by a diverse assemblage of angiosperms. It has been hypothesized that these species became isolated just after the Last Glacial Maximum, but this has not been tested. Additionally, the genetic diversity and population connectivity of the endemic Great Lakes flora has been understudied, with important conservation implications. Using genotyping-by-sequencing, we examined the relationship of P. occidentale subsp. lacustre to its closest relatives, relationships among all known populations, and genetic diversity within these populations. Polemonium occidentale subsp. lacustre represents an isolated, unique lineage that diverged from its closest relatives 1.3Ma and arrived in the Great Lakes Region by at least 38 ka. Nearly all extant populations diverged prior to the Last Glacial Maximum, are genetically distinct, and show little within-population genetic diversity. Clonality may mitigate reduction in diversity due to drift. Mixed population signal between Wisconsin and some Minnesota populations may be due to gene flow during the Late Pleistocene. While populations of P. occidentale subsp. lacustre may be relictual from a now extinct western relative, it is best treated as a distinct species. Conservation efforts should focus more on ensuring that current populations remain rather than maintaining large populations sizes across a few populations. However, encouraging habitat heterogeneity may accomplish both simultaneously.

Setting an agenda to catalyze research in the social and organizational dimensions of Great Lakes remediation, restoration, and revitalization

The Great Lakes region was once a hub of industry and innovation that provided wealth and identity to the region. Economic upheavals have left the region trying to recreate economies and cleanup degraded environments. There have been multiple, overlapping efforts to change these conditions and create a new narrative for the region through environmental remediation, habitat restoration, and community revitalization on the path towards resilience. The elements that contribute to success are organized differently in different places, and are not always identified or characterized in the environmental literature. Trying to fill this conceptual gap is critical because landscape-scale environmental cleanup has been delivered at the local scale through various partnerships and arrangements. Thus, this special collection of articles in the Journal of Great Lakes Research explores how individuals, organizations, and communities are engaging in the complex process of environmental cleanup and revitalization throughout the region. This collection of articles represents a range of approaches to unpack how people are navigating and contributing to this regenerative process from quantitative studies at the regional scale that characterize global patterns to in-depth qualitative studies that identify and characterize the processes that unfold in specific places to change our environments both ecologically and socially. These articles represent the broad experience unfolding in the region to understand these activities through research and navigate them through practice. This collection will add new dimensions to Great Lakes research by including the individuals, organizations, and agencies as components of the ecosystem.

Local environmental conditions influence species replacement in Great Lakes interdunal wetland macroinvertebrate communities

Abstract Understanding the role of environmental filtering and spatial processes along environmental gradients in assembling and maintaining aquatic communities in rare habitats is crucial for land management and biological conservation. We investigated the relative roles of environmental and spatial factors influencing beta (β) diversity of aquatic macroinvertebrate assemblages in 36 interdunal wetlands from five freshwater sand dune areas across two ecoregions spanning the latitudinal gradient of Lake Michigan. We hypothesised that aquatic macroinvertebrate diversity and composition would vary along the coastline because of an underlying environmental gradient. We predicted high species replacement correlated with environmental (local and regional) conditions and increasing species diversity with decreasing latitude. We calculated sample completeness, obtained diversity estimates based on Hill numbers and used abundance‐based β partitioning and multivariate analysis to examine β diversity, and its replacement and nestedness components in relation to local and regional predictors. Despite a short latitudinal gradient, we detected a significant increase in species richness with decreasing latitude, underpinned by a strong temperature and precipitation gradient. Species replacement (balanced variation in abundance) was high at all spatial scales (wetland, dune area, ecoregion and coastline), and correlated with local and regional environmental variables. Community dissimilarity showed no marked increase with spatial extent, which suggests a system where local‐scale environmental filtering prevents dispersal driven homogenisation. Both local and bioclimatic factors were correlated with aquatic macroinvertebrate dissimilarity, but local factors played a larger role. This study simultaneously examined the response of alpha and β diversity to geographical and environmental variables. Our results indicate that macroinvertebrates respond to abiotic factors by tracking suitable environmental conditions among locally variable interdunal wetlands. Thus, high dispersal along the coastline conveys resiliency to a hydrologically dynamic wetland system, which allows aquatic macroinvertebrates to contribute an integral portion of sand dune biodiversity within the Laurentian Great Lakes region. The high species turnover found suggests that conservation strategies should consider coastline connectivity among dune areas to maintain freshwater biodiversity.

Practical application of disease risk analysis for reintroducing gray wolves (Canis lupus) to Isle Royale National Park, USA

AbstractEvaluation of disease risks associated with wildlife translocations is important for minimizing unintended harm and achieving conservation goals. A framework for disease risk analysis (DRA) has been developed by the World Organization for Animal Health and International Union for Conservation of Nature, but applications for planning and implementation in wildlife conservation projects are limited. To fill this gap, we describe a DRA we conducted to identify, assess, and mitigate disease risks associated with reintroduction of gray wolves (Canis lupus) to Isle Royale National Park (IRNP). A total of 19 wolves were translocated from multiple locations within the Great Lakes Region to IRNP between September 2018 and September 2019. Integration of the DRA into project planning and use of diverse expertise among project personnel enabled a timely and cost‐effective process that facilitated multidisciplinary and cross‐cultural collaboration, transparent communication about risks and uncertainties, and practical management of disease risks for wildlife and personnel. Engaging disease experts and experienced field biologists in the assessment also helped to identify and account for potential sources of bias. We hope practical examples like this encourage wider adoption of DRA principles in translocations of wildlife for conservation purposes.

Planning for Climate Migration in Great Lake Legacy Cities

AbstractThe possibility that climate change might make the Great Lakes region (GLR) a more attractive place for people to live has gained traction and attracted media attention. Compared with the dry Southwest, the storm‐ridden Gulf region and the sea‐level rise exposed East and West Coasts, the GLR may fare relatively favorably due to an abundance of natural resources and projected climate amenities. While the emergence and character of such migration is still uncertain, it is essential that GLR urban communities proactively prepare and plan for such a potential future. Understanding how these shifts might affect residents of GLR communities will be critical for a just and sustainable future and for avoiding exacerbating existing inequalities and climate vulnerabilities. Here we propose new scalable methodologies for inclusive engagement that enable wide‐reaching knowledge co‐creation (e.g., web‐based engagement) that can meet the emergent and diverse challenges communities will face. These methodologies have the potential to not only broaden participation and improve practitioners' understanding of different GLR communities' preferences, but also to anticipate emerging tensions and potential synergies associated with increased population pressures.

Recently Amplified Interannual Variability of the Great Lakes Ice Cover in Response to Changing Teleconnections

Abstract The interannual variability of the annual maximum ice cover (AMIC) of the Great Lakes is strongly influenced by large-scale atmospheric circulations that drive regional weather patterns. Based on statistical analyses from 1980 to 2020, we identify a reduced number of accumulated freezing degree days across the winter months in recent decades, a step-change decrease of AMIC after the winter of 1997/98, and an increased interannual variability of AMIC since 1993. Our analysis shows that AMIC is significantly correlated with El Niño–Southern Oscillation (ENSO), the North Atlantic Oscillation (NAO), and the Pacific–North American pattern (PNA) before the winter of 1997/98. After that, the AMIC is significantly correlated with the tropical–Northern Hemisphere pattern (TNH) and eastern Pacific oscillation (EPO). Singular value decomposition of the 500-hPa geopotential height and surface air temperature shows a dipole pattern over the northeast Pacific and North America, demonstrating the ridge–trough system. This dipole pattern shifts northward to the northern Rocky Mountains, placing the Great Lakes region in the trough after 1997/98. This shift coincides with the increased interannual variability of the EPO index, as well as the change in the sea surface temperature (SST) over the northeast Pacific, where the second mode of the empirical orthogonal function (EOF) on SST shows a warm blob-like feature manifested over the Gulf of Alaska. The regression of wave activity flux onto the SST EOF shows that the source of upward and eastward propagation of a stationary Rossby wave shifts to the west coast of North America, likely moving the ridge–trough system eastward after the winter of 1997/98. Significance Statement We found that the Great Lakes annual maximum ice cover decreased after one of the strongest El Niño–Southern Oscillation events in 1997/98 and that its year-to-year fluctuations increased in the recent 20 years. After the winter of 1997/98, the Great Lakes annual maximum ice cover started to correlate with the warm sea surface temperature anomaly in the northeast Pacific, which appears to disrupt the polar vortex far up in the stratosphere, and the polar vortex ultimately shifts eastward the ridge–trough system over North America. When the shifted system develops enough, it can encase the Great Lakes region in the Arctic air and thereby cause larger year-to-year fluctuations. This connection was found to be characteristic in the recent few decades, after the winter of 1997/98.

Changes in abrupt alternations between wet and dry over the Great Lakes Region of Central Asia during the period 1976–2015

• GLRCA experienced an overall wetting trend. • Both D-W and W-D alternation events slightly decreased at the regional scale. • DWAA events decreased in 1976–1995 but increased in 1996–2015. • The variation of DWAA events was closely related to the dry and wet months. Under climate warming, the frequency of dry-wet abrupt alternation (DWAA) events was largely increased at the global scale. In this study, we identified DWAA events, including dry-wet (D-W) alternation events and wet-dry (W-D) alternation events, based on the Standardized Precipitation Evapotranspiration Index (SPEI) for the Great Lakes Region of Central Asia (GLRCA), and analyzed temporal and spatial variation of DWAA during the period 1976–2015. Over the entire study period, GLRCA experienced an overall wetting trend, and a heavy dry trend was mainly distributed in the southwest. Although both D-W alternation events and W-D alternation events showed a slight decrease at the regional scale, there were two clearly opposite trends between 1976 and 1995 and 1996–2015, with a decrease in 1976–1995 and an increase in 1996–2015. Spatially, the variation in the number of D-W alternation events and W-D alternation events exhibited a large heterogeneity. A significant increase in the frequency of D-W alternation events and W-D alternation events was observed in the southwest of arid climate zone, which may be attributed to the increased frequency of dry months and wet months in the region. This study in DWAA events is of importance to the implementation of water resources management, disaster prevention and mitigation policies in the GLRCA under the challenge of future climate change.

Farm-level Losses and Grower Willingness to Try Management Strategies for Swede Midge in Vegetable Crops

Since its introduction to North America in the 1990s, the invasive swede midge (Contarinia nasturtii) has become an important pest of cruciferous (Brassicaceae) vegetables in the northeast and Great Lakes regions of the United States and the Canadian provinces of Québec and Ontario. Swede midge reduces yield in cruciferous vegetables through larval feeding that distorts growth. Overlapping generations, cryptic larval feeding, and lack of effective biopesticides pose challenges for managing swede midge effectively using current tools. In 2018, we distributed an online survey for commercial vegetable growers in the United States and Canada to measure farm-level economic impacts of swede midge and grower perspectives on new management strategies for this pest. Growers reported losing $3808 US ($4890 Canadian) on average per acre per year due to swede midge–related vegetable crop losses. Both organic and conventional growers expressed an interest in paying more for nonchemical swede midge management vs. insecticides and were interested in trying new management strategies, particularly biological control.

Evaluation of fatty acid and antioxidant variation in a complex pasture system as compared to standard cattle feed in the Great Lakes region

As the demand for grass-fed ruminant products keeps increasing, more data are needed to assess the nutritional value of feedstuffs, especially pastures. In addition, global climate change adds another challenge to the management of grasslands with projections of changing temperature and precipitation patterns. Consequently, the variations in bioactive compounds such as fatty acids and antioxidants in feeds will be harder to predict. Therefore, it is critical to report region and time-specific results of the nutritional value of feeds intended for ruminant nutrition. The objectives of this study were to compare the antioxidant and fatty acid content of commonly used feedstuffs including a complex pasture mixture from the Great Lakes Region and a traditional grain-based diet, and to assess the variations of these bioactive compounds in the pasture over the course of two grazing seasons. Weather parameters including temperature and rainfall were recorded for the length of the study. Feed samples were collected between June and September 2019 and 2020 and analyzed for nutrient composition, chlorophyll A and B, carotenoids, and total phenols. Fatty acids were analyzed by GC-MS. Correlations were reported to analyze the relationship between individual plant species, antioxidants, and fatty acids. We observed higher antioxidant parameters in the pasture compared to the grain diet. Total polyunsaturated fatty acids were higher in the pasture including α-linolenic acid while the grain diet was higher in n-6 polyunsaturated fatty acids including linoleic acid. The n-6:n-3 ratio was more beneficial in the pasture and was 50–90 times higher in the grain diet. Variations in the fatty acid profile of the pasture were observed and varied between 2019 and 2020. Plant growth cycles, climatic conditions, and grazing methods were hypothesized to cause these changes. Altogether, this study increased our knowledge about the nutritional value of feedstuffs and will help ranchers and researchers to better understand the variations of bioactive content based on region, season, and climatic conditions.

Common Mudpuppy (Necturus maculosus (Rafinesque, 1818)) in Western New York: A Seasonal Comparison of Diet, Body Condition, and Capture Methods

The Common Mudpuppy (Necturus maculosus (Rafinesque, 1818)) is found in many watersheds from eastern Canada to the southeastern United States. Although its range is large, recent observations suggest its population is dwindling in the Great Lakes region. A lack of understanding about its distribution at a finer scale or whether diet and body condition exhibit seasonal patterns limits our ability to develop a conservation management plan. This study investigated seasonal changes in Common Mudpuppy diets and body condition across western New York (USA) over a 2-yr period by using rock turning (RT) and trapping (TR) collection methods. Common Mudpuppies were found in all four major watersheds of the region in both lentic and lotic habitats. RT was more efficient than TR in streams, whereas TR was a better option in lakes. Male-to-female sex ratios and four of five measured morphological features did not differ between collection methods, although the largest and smallest Common Mudpuppies were captured by RT, suggesting some size selectivity in TR. Body girth was significantly smaller for Common Mudpuppies collected by RT, and this was attributed to seasonal differences in activity. Stomach contents were varied and differed by season, with 41 unique prey types recovered, including several forms of microplastics. In summer and fall, Common Mudpuppies fed on invertebrates exclusively, but during winter and spring, fish were incorporated into the diet. Body condition reflected the change in diet, with a higher body condition when fish were present in the diet. These findings suggest seasonal data are necessary to fully understand Common Mudpuppy conservation needs.