Gray Hair Research Articles

The possibilities of correction of premature greying of hair and involutive hair changes

The review highlights the available published data on the etiopathogenesis of early graying, hair involution and restoration methods. Early graying of hair is defined as settlement before the age of 20 in Caucasians, before the age of 25 in Asians and before the age of 30 in Africans. In etiopathogenesis, an imbalance between oxidative stress and the antioxidant system is considered as the leading mechanism, a significant role is played by genetic predisposition, hormonal disorders of the thyroid gland, acute stress; the causes may also be deficiency of vitamin B12, copper, iron. Currently, the active molecule palmitoyl tetrapeptide-20 is used to prevent pigment loss and restore it. The endocannabinoid system in the hair follicle is also considered as a target for stimulation during the restoration of hair growth.

Estimation for Raw Material Plants of a Henna Product Using LC-High Resolution MS and Multivariate Analysis.

Henna is a plant-based dye obtained from the powdered leaf of the pigmented plant Lawsonia inermis, and has often been used for grey hair dyeing, treatment, and body painting. As a henna product, the leaves of Indigofera tinctoria and Cassia auriculata can be blended to produce different colour variations. Although allergy from henna products attributed to p-phenylenediamine, which is added to enhance the dye, is reported occasionally, raw material plants of henna products could also contribute to the allergy. In this study, we reported that raw material plants of commercial henna products distributed in Japan can be estimated by LC-high resolution MS (LC-HRMS) and multivariate analysis. Principal Component Analysis (PCA) score plot clearly separated 17 samples into three groups [I; henna, II; blended henna primarily comprising Indigofera tinctoria, III; Cassia auriculata]. This grouping was consistent with the ingredient lists of products except that one sample listed as henna was classified as Group III, indicating that its ingredient label may differ from the actual formulation. The ingredients characteristic to Groups I, II, and III by PCA were lawsone (1), indirubin (2), and rutin (3), respectively, which were reported to be contained in each plant as ingredients. Therefore, henna products can be considered to have been manufactured from these plants. This study is the first to estimate raw material plants used in commercial plant-based dye by LC-HRMS and multivariate analysis.

PENGARUH PEMBERIAN LABU SIAM TERHADAP PENURUNAN TEKANAN DARAH

Hyepertension in Indonesia is caused by a lifestyle that consumes foods with higt cholesterol levels, foods with higt salt content, lack of exercise and cannot be separated from genetic factors. Elderly is a period where individuals have entered a period of physical decline which is characterized by wrinkled skin, gray hair, missing teeth, hearing loss, blurred vision, and excessive body proportions, so that it is said that elderly are individuals in this age group who have entered a period of physical decline final stage of their life.

A case study of Duchenne muscular dystrophy caused by Alu element insertion in DMD gene and analysis of its gray-hair symptoms.

Duchenne muscular dystrophy (DMD) is a severe X-linked recessive genetic disorder caused by mutations in the DMD gene, which leads to a deficiency of the dystrophin protein. The main mutation types of this gene include exon deletions and duplications, point mutations, and insertions. These mutations disrupt the normal expression of dystrophin, ultimately leading to the disease. In this study, we reported a case of DMD caused by an insertion mutation in exon 59 (E59) of the DMD gene. The affected child exhibited significant abnormalities in related biochemical markers, early symptoms of DMD, and multiple gray hair. His mother and sister were carriers with slightly abnormal biochemical markers. The mother had mild clinical symptoms, while the sister had no clinical symptoms. Other family members were genetically and physically normal. Sequencing and sequence alignment revealed that the inserted fragment was an Alu element from the AluYa5 subfamily. This insertion produced two stop codons and a polyadenylate (polyA) tail. To understand the impact of this insertion on the DMD gene and its association with clinical symptoms, exonic splicing enhancer (ESE) prediction indicated that the insertion did not affect the splicing of E59. Therefore, we speculated that the insertion sequence would be present in the mRNA sequence of the DMD gene. The two stop codons and polyA tail likely terminate translation, preventing the production of functional dystrophin protein, which may be the mechanism leading to DMD. In addition to typical DMD symptoms, the child also exhibited premature graying of hair. This study reports, for the first time, a case of DMD caused by the insertion of an Alu element into the coding region of the DMD gene. This finding provides clues for studying gene mutations induced by Alu sequence insertion and expands the understanding of DMD gene mutations.

Assessment of Serum Ischemia Modified Albumin Level and Erectile Functions in Individuals with Premature Greying of Hair

Assessment of Serum Ischemia Modified Albumin Level and Erectile Functions in Individuals with Premature Greying of Hair

‘Old, weak, and confused’: A pilot study on perceptions of older adults and anxiety about aging among Kinh adults in rural Vietnam

ABSTRACT This study aims to explore Kinh Vietnamese adults’ perception of older adults, and examine their anxiety about ageing and its associated factors. Purposive sampling method was used to recruit rural community members who aged from 18 to 59 by the time of the study and self-identified with Kinh ethnicity, the major ethnicity in Vietnam. A total of 322 eligible participants completed face-to-face, semi-structured interviews conducted by research assistants. The survey consisted of both close-ended questions and open-ended questions to collect quantitative and qualitative data. The qualitative results from open-ended questions show that ‘older adults’ were perceived and described with overwhelmingly negative characteristics regarding their appearance (wrinkles, gray hair), physical and cognitive health conditions (weak, frail, confused), capacity (incapable of working), and personality and behavioral characteristics (being difficult and irritable). The quantitative findings show that contact with older adults, correct understanding of qualified age of older adults, and worry about getting dementia and caring for someone with dementia in the future were significantly associated with anxiety about aging. Stereotype and prejudice about older adults, as well as anxiety about aging are critical issues among Vietnamese adults. These results have their implications for educational programs and media campaigns to address ageist stereotypes and aging anxiety in community.

Deciphering potential causative factors for undiagnosed Waardenburg syndrome through multi-data integration

BackgroundWaardenburg syndrome (WS) is a rare genetic disorder mainly characterized by hearing loss and pigmentary abnormalities. Currently, seven causative genes have been identified for WS, but clinical genetic testing results show that 38.9% of WS patients remain molecularly unexplained. In this study, we performed multi-data integration analysis through protein-protein interaction and phenotype-similarity to comprehensively decipher the potential causative factors of undiagnosed WS. In addition, we explored the association between genotypes and phenotypes in WS with the manually collected 443 cases from published literature.ResultsWe predicted two possible WS pathogenic genes (KIT, CHD7) through multi-data integration analysis, which were further supported by gene expression profiles in single cells and phenotypes in gene knockout mouse. We also predicted twenty, seven, and five potential WS pathogenic variations in gene PAX3, MITF, and SOX10, respectively. Genotype-phenotype association analysis showed that white forelock and telecanthus were dominantly present in patients with PAX3 variants; skin freckles and premature graying of hair were more frequently observed in cases with MITF variants; while aganglionic megacolon and constipation occurred more often in those with SOX10 variants. Patients with variations of PAX3 and MITF were more likely to have synophrys and broad nasal root. Iris pigmentary abnormality was more common in patients with variations of PAX3 and SOX10. Moreover, we found that patients with variants of SOX10 had a higher risk of suffering from auditory system diseases and nervous system diseases, which were closely associated with the high expression abundance of SOX10 in ear tissues and brain tissues.ConclusionsOur study provides new insights into the potential causative factors of WS and an alternative way to explore clinically undiagnosed cases, which will promote clinical diagnosis and genetic counseling. However, the two potential disease-causing genes (KIT, CHD7) and 32 potential pathogenic variants (PAX3: 20, MITF: 7, SOX10: 5) predicted by multi-data integration in this study are all computational predictions and need to be further verified through experiments in follow-up research.

SASH1 S519N Variant Links Skin Hyperpigmentation and Premature Hair Graying to Dysfunction of Melanocyte Lineage

A better understanding of human melanocyte (MC) and MC stem cell biology is essential for treating MC-related diseases. This study employed an inherited pigmentation disorder carrying the SASH1S519N variant in a Hispanic family to investigate SASH1 function in the MC lineage and the underlying mechanism for this disorder. We used a multidisciplinary approach, including clinical examinations, human cell assays, yeast 2-hybrid screening, and biochemical techniques. Results linked early hair graying to the SASH1S519N variant, a previously unrecognized clinical phenotype in hyperpigmentation disorders. Invitro, we identified SASH1 as a regulator in MC stem cell maintenance and discovered that TNKS2 is crucial for SASH1's role. In addition, the S519N variant is located in one of multiple tankyrase-binding motifs and alters the binding kinetics and affinity of the interaction. In summary, this disorder links both gain and loss of pigmentation in the same individual, hinting to accelerated aging in human MC stem cells. The findings offer insights into the roles of SASH1 and TNKS2 in MC stem cell maintenance and the molecular mechanisms of pigmentation disorders. We propose that a comprehensive clinical evaluation of patients with MC-related disorders should include an assessment and history of hair pigmentation loss.

‘Hair Color Repigmentation in the Recipient Area‘: A Newly Recognized Phenomenon after Hair Transplantation

Background: Hair color repigmentation in the recipient area is a newly detected phenomena after hair transplantation. It’s detected especially in patients with gray or white hairs in the donor area, in which transferring these follicles to the recipient area stimulate them to regain their original blackish color, this may occur due to various stimulants that release during wound healing of slits after hair transplantation that trigger melanin production within the melanocyte inside the hair roots and promote hair repigmentation in the recipient area. Aim: The aim of this study was to detect the frequency of hair color repigmentation phenomena in the recipient area after hair transplantation. Patients and Methods: In this retrospective observational study, we detect thirty-four patients that develop hair color restoration phenomena in the recipient area after undergoing hair transplantation throughout a 1-year follow-up period. 1438 patients that undergo hair transplant between August 2021 and May 2023 were enrolled in this study among them 34 patients with white or gray hair in the donor area were vulnerable to hair color restoration phenomena, each patient was assessed both objectively and subjectively to evaluate the frequency of occurrence of this phenomenon and the percentage of hair follicles restore their original blackish color that transplanted to the recipient area. Results: Thirty-four patients with white or gray hair in the donor area were enrolled in this study that completed 1-year follow-up period. The evaluation score includes (percentage of patients show hair color restoration in the recipient area after hair transplantation, percentage of hair follicles show change in their original color, degree of color restoration among transplanted follicles in the recipient area) and patient’s satisfaction score showed greater positive results in which the majority of the patients with white or gray hair in the donor area restore their original blackish color of their hair after transplanted to the recipient area. Conclusion: Hair color repigmentation in the recipient area is a newly detected phenomena after hair transplantation surgery. Most patients with white or gray hair in the donor area show regain their original blackish color of their hair follicles in the recipient area after hair transplantation. We think that detecting this phenomenon will give us insight about how we can restore the original color of our white or gray hair by understanding the mechanism of how the melanocytes within the hair root regain their ability of melanin production after hair transplantation.

Konsep Uban dalam Tafsir Al Munir

This research was conducted to find out what is meant by gray hair and what is the concept of gray hair in Tafsir Al-Munir, gray hair comes from the words شَابَ - يَشِيْبُ - شَيْب, which means white hair on the top of the head, either a little or a lot. The plural form of the word شَيْب is شيُوْب. The word شَيْب in the Koran is found in three suras, including surah Maryam [19] verse 4 with the pronunciation شَيْبا surah al-Rûm [30] verse 54 with the pronunciation شَيْبةَ, and surah al-Muzzammil [73] verse 17 with the pronunciation شِيْب ا. Gray hair is hair that is white or starting to turn white. Gray hair is described in the Koran as a biological signal indicating that a person is old, as well as a theological signal that a person will soon be returned to his Lord. This research examines what is meant by gray hair and the concept of gray hair in Al Munir's interpretation. This research uses qualitative research methods and library research. From the results of this research, it can be concluded that the concept of gray hair in Wahba Al-Zuhaili's Tafsir Al-Munir is a reminder for those of us who will have gray hair to introspect ourselves about the message of its growth and implies obedience, even for every gray hair that grows in between. Between our hair there is a pile of wise advice to equip us to follow the path of life, to strengthen our beliefs, give birth to wisdom, make the sad happy, dispel despair, and give birth to a million hopes.

Intraoral laser hair removal: A scoping review.

Intraoral hair growth is a debilitating side effect of flap or graft-based oropharyngeal reconstruction. There is no standardized treatment, but groups have successfully eradicated unwanted hair growth with intraoral laser hair removal. This scoping review assesses the utility of laser therapy in managing this condition. This scoping review followed PRISMA-ScR guidelines. Ovid Medline, Embase (Ovid) and Scopus were queried using index terms and keywords. Resulting articles were reviewed for inclusion by two independent reviewers against inclusion criteria and relevant data were extracted. The literature search yielded 297 articles, 22 of which met inclusion criteria. In total, 77 patients were treated: 38 patients with an Alexandrite laser, 19 with an Nd:YAG laser, 18 with a diode laser, and two with a CO2 laser. Complete response defined as 80% or more reduction in hair count was achieved in 70 patients (90%) and six patients (8%) achieved a partial response (10%-79% reduction in hair count). One patient (1%) with gray hair saw less than a 10% reduction in hair count. On average, 3.84 treatment sessions were needed, spaced 5.4 weeks apart. Treatments were well tolerated without major side effects. This is the first scoping review assessing the utility of intraoral laser hair therapy and suggests it may be a safe and effective treatment. However, surgeons should advise preoperative hair removal when clinically feasible to mitigate this side effect as much as possible.

Unobtrusive Health Hazard

Oxidative stress is a biological mechanism triggered by an imbalance between the biosynthesis and accumulation of reactive oxygen species (ROS) in a biological system. Normally, ROS are produced as byproducts of oxygen metabolism and fulfill various physiological roles such as cell signaling. However, environmental stressors like UV radiation, ionizing radiation, pollutants, heavy metals, and xenobiotics can also lead to increased ROS production. Common types of ROS include superoxide radicals (O2−), hydrogen peroxide (H2O2), hydroxyl radicals (•OH), and singlet oxygen (1O2), which are generated during various metabolic pathways. Key cellular processes such as protein phosphorylation, activation of transcriptional factors, apoptosis, immunity, and differentiation depend on maintaining appropriate levels of ROS within cells. Under conditions of excessive ROS production, which can occur during both normal physiological processes and pathological conditions like inflammation, ischemia, and aging, ROS can damage important cellular components such as proteins, lipids, and nucleic acids. This production is primarily mitochondrial but can also result from enzymatic activities involved in the respiratory chain, prostaglandin synthesis, phagocytosis, and the cytochrome P450 system. Both immune system activation and environmental factors contribute to endogenous and exogenous free radical production. The signs of oxidative stress include fatigue, memory loss, muscle or joint pain, the appearance of wrinkles and grey hair, decreased eyesight, headaches, increased sensitivity to noise, and a heightened susceptibility to infections. To reduce oxidative stress, it is advised to avoid sugar and processed foods while balancing blood sugar levels, prevent infections, incorporate daily stress reduction practices, avoid toxins, and promote the production of antioxidants through diet and herbal supplements that are rich in antioxidants.

Fractal Pattern in the Premature Graying of Hair: A Case Report

Fractal Pattern in the Premature Graying of Hair: A Case Report

Anti-aging mechanism and effect of treatment with raw and wine-steamed Polygonatum sibiricum on D-galactose-induced aging in mice by inhibiting oxidative stress and modulating gut microbiota.

Polygonatum sibiricum (PS) is a traditional Chinese medicine (TCM) first recorded in Mingyi Bielu. The book documents that PS can nourish five internal organs, be taken for a long time, relax the body and prolong lifespan. Presently, PS is widely used in TCM to prevent premature graying of hair. Based on TCM theory and clinical trials, the wine steaming processed product from PS provides a better effect. However, no published study has elucidated the anti-aging mechanism. The study aim was to investigate the anti-aging mechanism of PS and its wine steaming processed product in mice, specifically focusing on the effect of D-galactose (D-gal) surrounding the intestinal flora and the Kelch-like ECH-associated protein 1-nuclear factor erythroid 2-related factor 2-antioxidant response elements (Keap1/Nrf2/ARE) pathway. The chemical components in Raw PS (RPS) and Wine-steamed PS (WPS) were identified by ultra-performance liquid chromatography-hybrid quadrupole-Orbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap HRMS). An aging model using Kunming mice was established through intraperitoneally injected D-gal. Concentrations of RPS and WPS at 5, 10, or 15g/kg/day levels were administered intragastrically, respectively. The body weight, liver and spleen indexes, superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and malondialdehyde (MDA) activities in serum and brain tissue were recorded. Hematoxylin and eosin (HE) stained brain tissue was histopathologically examined. The expressions of Keap1, Nrf2 and heme oxygenase 1 (HO-1) in the brain tissue at the mRNA and protein levels were respectively detected by reverse transcription-polymerase chain reaction (RT-PCR) and western blot (WB). Moreover, an Illumina Hiseq platform was used for 16S ribosomal RNA (16S rRNA) high-throughput sequencing to evaluate the proportions of intestinal flora in aging mice. The proportions of saccharides, flavonoids, and triterpene acids were different between RPS and WPS. In the aging model mice, WPS outperformed RPS in improving body weight and mental state by increasing the spleen index, SOD and GSH-PX activities, decreasing the liver index and MDA activities, and restoring the histopathological morphology in D-gal-induced aging mice. At the mRNA levels, RPS and WPS significantly reduced the expression of Keap1 and increased the expressions of Nrf2 and HO-1. The trend in protein expressions was similar to that of the mRNA results, and WPS had a stronger effect than RPS. Fecal microbiota analysis showed that RPS and WPS restored intestinal microbiota proportions to normal levels. The results demonstrated that PS and its WPS had a positive effect in relieving oxidative stress in aging mice. WPS outperformed RPS, which might be related to the activation of the Keap1/Nrf2/ARE pathway and regulation of intestinal flora.

Vitiligo Causes and Treatment: A Review

Vitiligo is a chronic autoimmune disorder characterized by patches of skin losing pigment due to the destruction of melanocytes, the cells responsible for producing pigment. The condition can affect people of all ages and ethnicities, with symptoms including milky-white patches on the skin, premature graying of hair, and color loss in mucous membranes like the mouth or nose. Among the main causes of vitiligo are; Autoimmune Response: Vitiligo is primarily an autoimmune disease where the immune system mistakenly attacks melanocytes, leading to depigmentation. Factors like family history, genetic predisposition, and immune system disorders contribute to its development, Triggers: Events such as sunburn, emotional distress, or exposure to certain chemicals can trigger or exacerbate vitiligo. The treatment options are; Medications: Treatments include light therapy (phototherapy), oral medications like psoralen combined with UVA light (PUVA), and depigmentation therapy using monobenzone to match skin tones Surgery: Surgical options are available for some cases of vitiligo and Psychological Support: Counseling and mental health services can help individuals cope with the emotional impact of vitiligo, in conclusion, v is a multifaceted skin condition lacking a conclusive remedy, many types of vitiligo, but the most common one is Non-segmental Vitiligo (NSV), honmental and genetic are the most important causes of vitiligo. Important symptoms of vitiligo are; depigmented patches, hair discoloration, sensitivity to sunlight, treatment of vitiligo have many approach, among them are; topical Corticosteroids, phototherapy and Cosmetic Camouflage.

Melanocyte stem cells in the skin: Origin, biological characteristics, homeostatic maintenance and therapeutic potential.

Melanocyte stem cells (MSCs), melanocyte lineage-specific skin stem cells derived from the neural crest, are observed in the mammalian hair follicle, the epidermis or the sweat gland. MSCs differentiate into mature melanin-producing melanocytes, which confer skin and hair pigmentation and uphold vital skin functions. In controlling and coordinating the homeostasis, repair and regeneration of skin tissue, MSCs play a vital role. Decreased numbers or impaired functions of MSCs are closely associated with the development and therapy of many skin conditions, such as hair graying, vitiligo, wound healing and melanoma. With the advancement of stem cell technology, the relevant features of MSCs have been further elaborated. In this review, we provide an exhaustive overview of cutaneous MSCs and highlight the latest advances in MSC research. A better understanding of the biological characteristics and micro-environmental regulatory mechanisms of MSCs will help to improve clinical applications in regenerative medicine, skin pigmentation disorders and cancer therapy. KEY POINTS: This review provides a concise summary of the origin, biological characteristics, homeostatic maintenance and therapeutic potential of cutaneous MSCs. The role and potential application value of MSCs in skin pigmentation disorders are discussed. The significance of single-cell RNA sequencing, CRISPR-Cas9 technology and practical models in MSCs research is highlighted.

Therapeutic modulation of KIT ligand in melanocytic disorders with implications for mast cell diseases.

KIT ligand and its associated receptor KIT serve as a master regulatory system for both melanocytes and mast cells controlling survival, migration, proliferation and activation. Blockade of this pathway results in cell depletion, while overactivation leads to mastocytosis or melanoma. Expression defects are associated with pigmentary and mast cell disorders. KIT ligand regulation is complex but efficient targeting of this system would be of significant benefit to those suffering from melanocytic or mast cell disorders. Herein, we review the known associations of this pathway with cutaneous diseases and the regulators of this system both in skin and in the more well-studied germ cell system. Exogenous agents modulating this pathway will also be presented. Ultimately, we will review potential therapeutic opportunities to help our patients with melanocytic and mast cell disease processes potentially including vitiligo, hair greying, melasma, urticaria, mastocytosis and melanoma.

ASSOCIATION OF VITAMIN B12 DEFICIENCY AND MUCO-CUTANEOUS FINDINGS IN DERMATOLOGY: A CASE-CONTROL STUDY

Background:Vitamin b12 deficiency is considera common nutritional deficiency in Indian population.Vitamin B12 deficiency related to hair and cutaneous features like hair-fall, and cutaneous pigmentation are not well described in literature. Aim:To study serum vitamin b12 level in relation to diffuse hair-fall and muco-cutaneouspigmentation. Study design: Case-control observational study Materials and methods:Total 240 patients presented with diffuse hair-fall or muco-cutaneous pigmentation were taken as cases and screened for serum vitamin b12 level at tertiary health care centre. Other 240 patients of same age group presented with other dermatological conditions were taken as control. Also associated symptoms like weakness, malaise, numbness, tingling, paresthesia, burning sole, glossitis, angular cheilitis, angular stomatitis, aphthous stomatitis, graying of hair, mild memory impairment, irritability, psychosis, delusion of paresthesia, dementia and depression were noted in each patient. Results:Out of 240 patient females: male ratio was 3.52:1. Vegetarian:non-vegetarian ratio was 3.36:1. Out of 240 patients 42% patient presented with hair fall, 33% with hyperpigmentation and 25% had both complain. Facial pigmentationin 20%, extra-facial in 15%, both (facial & extra-facial) in 45% of patients were noted. Mucosal pigmentation was present in 20%. Serum vitamin b12 level was low in 198 cases and 87 control. Hemoglobin level was low (<10 gm/dl) in 196 0f cases and 127 of control. Amongst 240 cases weakness in 58, malaise in 22, numbness in 5, tingling in 15, paresthesia in 2, burning sole in 108, glossitis in 76, angular stomatitis in 13, aphthous ulcer in 31, graying of hair in 92, memory impairment in 18, irritation in 64, psychosis and delusion of paresthesia in 1, dementia in 8 patients were noted. Conclusions:Vitamin B12 deficiency is not rare, but data are insufficient regarding its prevalence. In our study, 240 patients with diffuse hair fall and muco-cutaneous pigmentation as case group and 240 patients as control group were included. Among groups, vitamin b12 deficiency was found in 198 cases and 87 control. We found that there was significant association with vitamin b12 deficiency, diffuse hair fall and muco-cutaneous pigmentation as clinical markers for dermatologists.

A phytochemical and comparative study to assess the efficacy of Kaidarya (Murraya koenigii (L.) Sprengel) siddha taila plus Kaidarya vati and Kaidarya vati in Akala palitya.

An urgent concern for the younger population is premature hair greying. Regardless of gender, it naturally occurs in the fourth decade, although these days, it strikes in the early twenties as well. According to Ayurveda, faulty eating habits, physical strain, psychological issues, environmental variables, and changes in lifestyle are chief reasons which leads to impaired digestion and Pitta dosha [associated with fire and water element] vitiation, particularly reduced production of Bhrajaka Pitta [biological pigment], which mostly leads to hair colour loss. Current study was phytochemical, comparative, interventional clinical study done to evaluate the efficacy of Kaidarya [Murraya koenigii (L.) Sprengel] siddha taila plus Kaidarya vati and Kaidarya vati alone in Akala palitya. Here, Group A patients were allotted to Kaidarya siddha taila plus kaidarya vati treatment whereas Group B patients were assigned with kaidarya vati alone. The sample was 84 but total enrolled subjects were 95 with respect to dropout rate. The paired t-test was used to analyse the data from Group A and Group B both before and after treatment. A noteworthy distinction was noted between the mean values obtained prior to and following therapy. An independent t-test was used to compare the results between Groups A and B. A significant difference in Akala palitya was found between Groups A and B. Group A's [Kaidarya siddha taila plus Kaidarya vati] mean difference score is higher than Group B's [Kaidraya vati]. Therefore, effect of Kaidarya siddha taila plus kaidarya vati is more than Kaidarya vati in the management of Akala palitya [premature greying of hair].

PHYSICOCHEMICAL AND PHYTOCHEMICAL ANALYSIS OF HERBO-MINERAL HAIR DYE FOR PREMATURE HAIR GRAYING

Background: Premature Hair Graying (PHG/ Canities) is the burning problem of today’s society. Despite extensive research, the treatment for PHG is still unsatisfactory. Ayurveda considers Palitya (premature hair graying) as an ailment of vitiated Pitta dosha (deranged functional factor of the body responsible for digestion and metabolism). Ayurveda therapeutics can provide the natural solution to this problem without adverse effects and also can prevent the recurrence. Objective: In present research work, attempts are made to formulate unique, safe and effective herbomineral hair dye (Keshranjak yog) an Ayurvedic formulation. The prepared formulation is also evaluated for its efficiency through various organoleptic, phytochemical and physicochemical parameters. Results: The pH of prepared herbomineral hair dye was found to be 6 which is close to neutral; to suit the requirements of different scalp and hair types. Total phenolic content (8.1%±0.7), total flavonoid content (6±1.2) of prepared sample were found to be acceptable and within limit. Conclusion: The dyeing effect of the developed dye may be attributed to keshya (good for hair), Kesharanjak (hair coloring) properties according to Ayurveda and presence of tannins, phenols as per pharmaceutics. Nowadays natural remedies are widely accepted as they are safer with minimal side effects as compared to the chemical hair dye. Kesharanjak yog may provide vital nourishment to the scalp skin and can color the hair in an utmost gentle manner. Due to nontoxic nature can act as public healthcare product.