Gravitational Changes Research Articles

Response of renal sympathetic nerve activity to parabolic flight-induced gravitational change in conscious rats

The renal sympathetic nerve activity (RNA) response to gravitational changes induced by parabolic flight was examined in chronically instrumented conscious rats. Two types of RNA responses were found. In six out of 12 rats, the RNA did not respond during the 2 G period, but immediately fell to background levels on entry into microgravity (μG), then recovered to the 1 G control level during continued μG (shutdown obvious group). In the other six rats, the RNA increased to 158±13% at the end of the 2 G period, increased further to 195±22% on entry into μG, then gradually recovered to that seen at 1 G (shutdown obscure group). The mean arterial pressure in the shutdown obvious group was significantly higher and the heart rate tended to be higher than in the shutdown obscure group, suggesting that the baseline sympathetic tone in the shutdown obvious group was higher than in the shutdown obscure group. These results suggest that the RNA response to parabolic flight might be affected by the baseline sympathetic tone.

Nontidal oceanic contributions to gravitational field changes: Predictions of the Parallel Ocean Climate Model

This study examines the nontidal contributions of the oceans to the Earth's gravitational field variations as predicted by a global ocean general circulation model: the Parallel Ocean Climate Model. Such variations in the gravitational field Stokes coefficient are determined up to spherical harmonic degree and order 20 and compared with satellite laser ranging (SLR) data from LAGEOS I and LAGEOS II. For most Stokes coefficients except the lowest‐degree ones, this investigation indicates that the application of sea level adjustment to reduce the effects of the model's lack of mass conservation due to the Boussinesq approximation has a negligible effect on timescales less than a few years. Predicted gravitational changes show strong seasonal variability and account for a portion of the variations estimated from SLR. We conclude that, in addition to the atmosphere, the oceans are an important contributor to the temporal variations in the Earth's gravitational field. The Stokes coefficients are useful in examining oceanic mass transport between hemispheres and ocean basins. The estimated oceanic power spectrum has a spectral shape similar to the atmosphere and is well above the noise level of planned satellite missions like the Gravity Recovery and Climate Experiment (GRACE).

The role of cytoskeleton in cell changes under condition of simulated microgravity

Single cells and cell culture are very good model for estimation of primary effects of gravitational changes. It is suggested that cell cytoskeleton plays a key role in mechanisms of adaptation to mechanical influences including gravitational ones. Our results demonstrated that cultured cells of human vascular endothelium are highly sensitive to hypogravity (clinorotation) and respond by significant decrease of cell proliferative activity. Simultaneously it was noted that the formation of confluent monolayer appeared early in cultures exposed to simulated microgravity due to accelerated cells spreading. Long-term hypogravity (several hours or days) leads to significant changes of cell cytoskeleton revealed as microfilament thinning and their redistribution within cell. Such changes were observed only in monolayer cells and not in cell suspensions. Gravitational forces as known to be modificators of cell adhesive ability and determine their mobility. Hypogravity environment stimulated endothelial cell migration in culture: 24–48 hrs pre-exposition to hypogravity significantly increased endothelial cell migration resulting in 2–3-fold acceleration of mechanically injured monolayer repair. Obtained results suggest that the effects of hypogravity on cultured human endothelial cells are, possibly, associated with protein kinase C and/or adenylate cyclase activity and are accompanied by noticeable functional cell changes.

Dome formation and tubule morphogenesis by Xenopus kidney A6 cell cultures exposed to microgravity simulated with a 3D-clinostat and to hypergravity.

Confluent high-density cell cultures of A6 cells derived from adult male Xenopus kidney exhibit spontaneous dome-formation at 1 g. To determine whether this morphogenetic property is altered by gravity, we used a three-dimensional (3D) clinostat to subject the cells to simulated microgravity, and a centrifuge to subject them to hypergravity. We used the generation orbit control method as the new rotation control system of the 3D-clinostat, not the random method. The growth of A6 cells was significantly enhanced by hypergravity, but significantly reduced by simulated microgravity. Dome formation by A6 cells at high confluence was inhibited under simulated microgravity conditions, whereas hypergravity promoted dome formation and induced tubule morphogenesis, compared to the control at 1 g. These results indicated that changes in gravity influence the morphogenetic properties of A6 cells, such as dome formation and tubule morphogenesis. When dome formation by A6 cells at high confluence was induced spontaneously in the control 1 g culture, the gene expression of the HGF family of pleiotropic factors, such as HGF-like protein (HLP) and growth factor-Livertine (GF-l.ivertine), an epithelial serine protease of channel activating protease 1 (CAP1), and Na+, K+-adenosine triphosphatase (ATPase), increased. Simulated microgravity increased the gene expression of activin A and reduced the gene expression of HLP, GF-Livertine, CAP1, and Na+, K+-ATPase. Hypergravity, on the other hand, decreased the gene expression of activin A and increased the gene expression of HLP, GF-Livertine, CAP1, and Na+, K+-ATPase. These results suggest that the effects of gravitational changes on expression of the HGF family member gene, CAP1, and Na+, K+-ATPase gene may be important for the cell growth, tubule morphogenesis, and dome formation of A6 cells in altered

Compression Therapy in Aeronautic Medicine

Aim: To review the influences of air and space travel on the venous side of the circulation and to assess the consequences for air and space travellers. Method: A review of current literature in the field of aeronautical travel has been conducted, with particular reference to the effects on the venous system and use of compression garments. Synthesis: The venous system is particularly sensitive to gravitational and pressure changes encountered during air and space travel due to the low pressures normally present in this part of the circulation. The symptoms experienced by air crew may be countered by the use of venous compression stockings, amongst other measures. More sophisticated techniques are required for fighter aviation and space flights. Aircraft and spacecraft travel in a hostile environment in which it is not possible for man to survive without sophisticated layers of protection. In addition, forces of acceleration generated by aircraft flight combine with those of the environment hazarding the safety and the health of navigators. Continuing technical advances allow increased performance towards ever greater speed, better acceleration, a greater range of action and longer periods of time in space. Conclusion: Increasingly, the limiting factor in air and space travel appears to be man, who has difficulty in tolerating the environment to which he is exposed. The immediate risk is loss of consciousness and in time there is the risk of health deterioration. The cardiovascular system as a whole is at the centre of this problem and seems to be one of the limiting factors in the design of more advanced aircraft, especially those used for military purposes.

Gravity and thermoregulation: metabolic changes and circadian rhythms.

Gravity appears to alter thermoregulation through changes in both the regulated level of body temperature and the rhythmic organization of temperature regulation. Gravity has been hypothesized to have an associated metabolic cost. Increased resting energy expenditure and dietary intake have been observed in animals during centrifuge experiments at hypergravity. Thus far, only animals have shown a corresponding reduction in metabolism in microgravity. Altered heat loss has been proposed as a response to altered gravitational environments, but remains documented only as changes in skin temperature. Changes in circadian timing, including the body temperature rhythm, have been shown in both hypergravity and microgravity, and probably contribute to alterations in sleep and performance. Changes in body temperature regulation may result from circadian disturbance, from the direct or indirect actions of gravity on the regulated temperature, or from changes in thermoregulatory effectors (heat production and heat loss) due to altered gravitational load and convective changes. To date, however, we have little data on the underlying thermoregulatory changes in altered gravity, and thus the precise mechanisms by which gravity alters temperature regulation remain largely unknown.

Kinetics of constant gravitropic stimulus responses in Arabidopsis roots using a feedback system.

The study of gravitropism is hindered by the fact that as a root responds, the gravitational stimulus changes. Using a feedback system to connect a rotating stage platform to a video digitizer system, we were able to maintain a constant angle of gravistimulation to Arabidopsis roots for long time periods. The rate of curvature approximated the sine rule for angles of stimulation between 20 degrees and 120 degrees. For a given angle of stimulation, the rate of curvature also remained constant, with no observed diminishment of the response. Although previous reports of Arabidopsis root gravitropism suggest latent periods of approximately 30 min, using a smooth mechanical stage to reorient the root, we observed a mean time lag of approximately 10 min. This more rapid onset of curvature can, in part, be explained by reduced mechanical perturbation during the process of gravistimulation. This suggests that mechanical stimulation associated with rapid root re-orientation may confound investigations of early gravitropic events.

Exposure to altered gravity affects all stages of endochondral cartilage differentiation

Chondrogenesis has a number of well-defined steps: (1) condensation, which involves cell aggregation, adhesion and communication; (2) activation of cartilage genes, which is accompanied by rounding up of the cells and intracellular differentiation; and (3) production and secretion of cartilage specific matrix molecules. Our studies show that each of these steps is affected by exposure to gravitational changes. Clinorotation and centrifugation affected initial aggregation and condensation. In the CELLS experiment, where cells were exposed to microgravity after some condensation occurred perflight, intracellular differentiation and matrix production were delayed relative to controls. Once cartilage has developed, in rats, further differentiation (hypertrophy, matrix production) was also affected by spaceflight and hind limb suspension. For the process of chondrogenesis to proceed as we know it, loading and other factors present at lg are required at each step of the process. This requirement means that not only will skeletal development and bone healing, processes involving chondrogenesis, be altered by long term exposure to microgravity, but that continuous intervention will be necessary to correct any defects produced by altered gravity environments.

重力荷重と骨代謝変化

重力荷重と骨代謝変化

Cosmological Parameters from the X-Ray Evolution of Clusters

This poster paper presents an extension of the entropy-driven model of cluster evolution developed by Bower, 1997 (MN, 288, 355) in order to study the evolution of clusters in a low density universe. Our approach allows us to explicitly seperate the contributions from gravitational collapse and changes in the core entropy of the intracluster gas. Here, we apply the model to determine whether a preferred value of Ω0 is selected by currently available constraints (Figure 1). Measurements of the X-ray evolution of clusters cannot, by themselves, select out a particular cosmological model. An additional constraint on the effective power spectrum index (n) is required. This can either come from a measurement of the large-scale galaxy correlation function or from the shape of the present-day temperature function. A theoretical calculation of the Ω0 dependence of the power spectrum does not help break the inherent degeneracy.

This Month in the Journal

This Month in the Journal

Effects of Gravitational Changes on the Bone System In Vitro and In Vivo

Spaceflight data obtained on bone cells, rodents, and humans are beginning to shed light on the importance of gravitational loading on the skeletal system. The space environment is a relevant model to explore the bone cell response to minimal strains. However, whether there is a direct effect of gravity on the cell rather than changes related to lack of convection forces in cell cultures performed in microgravity is unknown. In vitro studies carried out using osteoblastic cell cultures in space show changes in cell shape, suggesting that cell attachment structures as well as cytoskeleton reorganization might be involved. Valuable information is expected from in vitro models of an increase or decrease in mechanical stress in order to identify the different pathways of mechanoreception and mechanotransduction in the osteoblastic lineage. Results obtained from both humans and rodents after spaceflights indicated that bone mass changes are site specific rather than evenly distributed throughout the skeleton, thus emphasizing the need to perform measurements at different bone sites: weight- and non-weight-bearing bones, and cancellous and cortical envelopes. Bone mass measurements and biochemical parameters of bone remodeling are currently under evaluation in cosmonauts. Histomorphometric studies of bones from rats after space missions of various periods provided the time course of the cancellous bone cellular events: transient increase in resorption and sustained decrease in bone formation. The underlying bone loss occurred first in weight-bearing bones and later in less weight-bearing bones. During the postflight period, time required to recover the lost bone was greater than the mission length. Thus, the postflight period deserves more attention than it is currently receiving. On earth, the rat tail-suspension model is currently used to mimick spaceflight-induced bone loss. Data from the model confirmed the impairment of osteoblastic activity and showed an alteration in osteoblast recruitment with skeletal unloading. However, this model needs to be further validated.

On Mechanosensation in Bone Under Microgravity

Recent research has illuminated the biological response of bone to mechanical loading at the cellular level, but the precise mechanosensory system that signals bone cells to deposit or resorb tissue has not been identified. The purpose of this paper is to describe the current status of this research and to suggest some possible mechanosensory systems by which bone cells might sense environmental loads. The question of whether the mechanosensory system in bone tissue is at the level of the cell or whether it is at the tissue level and involving the cells is considered here. More precisely, the following question is addressed: can an osteocyte or an osteoblast read the gravitational field changes directly (and independent of changes in its environment), or does it detect those changes indirectly from its environment by contact stresses as it must detect other changes in mechanical loading on the surface of the earth? Our strategies for coping with the decay of the musculoskeletal system in long term space flight are somewhat dependent upon the answer to this question.

Response to hypogravity of normal in vitro cultured follicular cells from thyroid

Aim of this investigation is the study of molecular modifications occurring in differentiated mammalian cells exposed to gravitational changes. The test system chosen is a well characterized clone of differentiated, normal thyroid follicular cells (FRTL5) in long-term culture. As a follow-up to our recent experiment performed during the MASER-7 sounding rocket mission, flown for European Space Agency by Swedish Space Corporation in May 1996, we evaluated FRTL5 cells responses to Thyroid Stimulating Hormone dependent cAMP production under acute hypogravity conditions obtained in a fast rotating clinostat. Following this approach, we evaluated the FRTL5 cells response to TSH under microgravity conditions in order to optimize experimental tools and strategies in preparation to, and in between real flight missions.

Gravitational potential changes of a spherically symmetric earth model caused by a surface load

We define load Love numbers and derive expressions of Green's functions for calculating the vertical displacement, potential and gravity changes for any r. Numerical calculations are carried out for the 1066A earth model. Load Love numbers are obtained for three depths: r = a, r = 6334.4 km and r = 3484 km. The results show that the load Love numbers inside the earth decrease very fast as n increases. The smaller the radial distance r, the faster is the decrease of the load Love numbers with n. The load Love numbers inside the earth for degrees 2 and 20 indicate that a low degree load Love number has a global contribution and varies gently in magnitude along the radius, while a high degree load Love number reflects a regional or even a local contribution and it decreases very fast as the depth increases. The Green's functions of vertical displacement, potential and gravity changes for above mentioned three depths are also numerically calculated. All the Green's functions show that a deformation in near field is much larger than that in far field and the whole earth deforms under a surface mass load.

Changes in gravity inhibit lymphocyte locomotion through type I collagen.

Immunity relies on the circulation of lymphocytes through many different tissues including blood vessels, lymphatic channels, and lymphoid organs. The ability of lymphocytes to traverse the interstitium in both nonlymphoid and lymphoid tissues can be determined in vitro by assaying their capacity to locomote through Type I collagen. In an attempt to characterize potential causes of microgravity-induced immunosuppression, we investigated the effects of simulated microgravity on human lymphocyte function in vitro using a specialized rotating-wall vessel culture system developed at the Johnson Space Center. This very low shear culture system randomizes gravitational vectors and provides an in vitro approximation of microgravity. In the randomized gravity of the rotating-wall vessel culture system, peripheral blood lymphocytes did not locomote through Type I collagen, whereas static cultures supported normal movement. Although cells remained viable during the entire culture period, peripheral blood lymphocytes transferred to unit gravity (static culture) after 6 h in the rotating-wall vessel culture system were slow to recover and locomote into collagen matrix. After 72 h in the rotating-wall vessel culture system and an additional 72 h in static culture, peripheral blood lymphocytes did not recover their ability to locomote. Loss of locomotory activity in rotating-wall vessel cultures appears to be related to changes in the activation state of the lymphocytes and the expression of adhesion molecules. Culture in the rotating-wall vessel system blunted the ability of peripheral blood lymphocytes to respond to polyclonal activation with phytohemagglutinin. Locomotory response remained intact when peripheral blood lymphocytes were activated by anti-CD3 antibody and interleukin-2 prior to introduction into the rotating-wall vessel culture system. Thus, in addition to the systemic stress factors that may affect immunity, isolated lymphocytes respond to gravitational changes by ceasing locomotion through model interstitium. These in vitro investigations suggest that microgravity induces non-stress-related changes in cell function that may be critical to immunity. Preliminary analysis of locomotion in true microgravity revealed a substantial inhibition of cellular movement in Type I collagen. Thus, the rotating-wall vessel culture system provides a model for analyzing the microgravity-induced inhibition of lymphocyte locomotion and the investigation of the mechanisms related to lymphocyte movement.

Bifurcation of a capillary minimal surface in a weak gravitational field

In this paper we study a variational elliptic boundary-value problem on a convex region with Bond parameter that arises in hydromechanics and is closely related to the Plateau problem. It describes the behaviour of an elastic surface separating two liquid or gaseous media as the gravitational field changes. In the absence of gravitational force we have and the solution to the problem is a minimal surface. Here we study the behaviour of this surface (loss of stability, bifurcations) when gravity is introduced. The method of analysis is based on reducing the problem to an operator equation in Hölder or Sobolev spaces with a non-linear Fredholm operator of index 0 that depends on the parameter , and applying the Crandall-Rabinowitz theorem on simple bifurcation points, the Lyapunov-Schmidt method of reduction to finite dimensions, and the key function method due to Sapronov. We obtain both necessary and sufficient general conditions for bifurcation, and study in detail the situation when is a circle or a square.

This Month in the Journal

This Month in the Journal

Periodicity of magnetic intensities in magnetic anomaly profiles: the Cenozoic of the South Atlantic

SUMMARY Spectral analyses of several published magnetic anomaly profiles from Cande & Kent (1992a) were undertaken prior to analysing, in the same way, raw magnetic anomaly data from similar parts of the South Atlantic. It was found that similar and distinct medium and short wavelengths were present in both the published and raw data. When these are converted into the time domain using the average rate of spreading for each profile, these periodicities appear similar, possibly identical, to those expected from the long-term eccentricity orbital parameters ( Fischer, DeBoer & Premoli Silva 1990). While such correlations are not necessarily causative, they suggest that magnetohydrodynamical processes near the core-mantle boundary may be affected by gravitational changes due to planetary orbital perturbations.

Effects of extreme lateral posture on hemodynamics and plasma atrial natriuretic peptide levels in critically ill patients.

To quantify the hemodynamic effects of turning critically ill, mechanically ventilated patients to the extreme left and right lateral postures. Prospective investigation. Eight-bed intensive care unit in a university hospital. Twelve consecutive patients presenting with severe respiratory failure and requiring continuous positive inotropic support. All patients were mechanically ventilated and placed in a kinetic treatment system. They were positioned in the supine, left dependent, and right dependent postures, resting for 15 min in each position. Hemodynamic measurements, assessments of right ventricular function, and determinations of intrathoracic blood volume were performed in three different positions. Concentrations of atrial natriuretic peptide in plasma were quantified. In three patients, the findings were controlled by transesophageal echocardiography. Cardiac index [median (range) 5.5 (3.2-8.1) vs 4.3 (3.2-7.5) l/min per m2, p < 0.01], intrathoracic blood volume [1125 (820-1394) vs 1037 (821-1267) ml/m2, p < 0.01], and right ventricular end-diastolic volume [130 (83-159) vs 114 (79-155) ml/m2, p < 0.05] increased significantly in the left dependent position compared to supine. Mean arterial pressure did not change. Atrial natriuretic peptide levels rose from 140 to 203 pg/ml. In the right dependent position, we found a marked decrease in the mean arterial pressure [85 mmHg (supine) to 72 mmHg (right dependent), p < 0.01]. Cardiac index and intrathoracic blood volume were unchanged, but right ventricular end-diastolic volume decreased from 114 to 102 ml/m2 (p < 0.05). Additionally, atrial natriuretic peptide levels decreased significantly (median delta value: 37 pg/ml). In echocardiographic controls we found an increase in right ventricular end-diastolic diameters in the left dependent position and shortened diameters in the right dependent position. Extreme lateral posture affects the cardiovascular system in critically ill, mechanically ventilated patients: in the left dependent position a "hyperdynamic state" is reinforced, while the right decubitus position impairs right ventricular preload and predisposes to hypotension. Echocardiography and changes in plasma atrial natriuretic peptide values indicate that these findings are due to altered distensibility of the right ventricle caused by regional intrathoracic gravitational changes. We conclude that the duration and the angle of lateral posture should be restricted in hemodynamically unstable patients.