Let q be a prime power and suppose that e and n are integers satisfying 1 ⩽ e ⩽ n − 1. Then the Grassmann graph Γ( e, q, n) has as vertices the e-dimensional subspaces of a vector space of dimension n over the field F q , where two vertices are adjacent iff they meet in a subspace of dimension e − 1. In this paper, a characterization of Γ( e, q, n) in terms of parameters is obtained provided that e ≠ 2, n − 2, 1 2 n, 1 2 (n ± 1) and ( e ≠ 1 2 (n±2) if q ϵ {2, 3}) and (e ≠ 1 2 (n ± 3) if q = 3). As a consequence we can show that these Grassmann graphs are uniquely determined as distance-regular graphs by their intersection arrays.