The effect of incorporating d-glucosamine at various levels into the water-soluble, chemically defined diet No. 26 fed ad libitum to rats has been compared with that observed earlier under the same conditions when the inferior diet No. 3 was employed. As before, a linear graphical relationship between growth and food intake was observed which, by extrapolation, permitted a calculation of the average daily requirement of food for the maintenance of body weight over the entire course of the experiment. On the superior diet, No. 26, there was ( a) a slightly higher maintenance requirement, and ( b) appreciably less depression in the growth rate at equal levels of glucosamine, than on diet No. 3. No toxic symptoms were evident, and it is apparent that the dietary glucosamine simply exerts a self-limiting effect on food intake. Force-feeding of diet No. 26 containing 8 and 12% of glucosamine to rats bearing the transplanted Walker tumor resulted in no symptoms of toxicity nor any effect upon tumor or carcass growth. Observations on the cyclic daily weight fluctuations of animals ingesting soluble diets as compared with Purina Chow are described.
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