Progress in the diagnosis and treatment of human diseases is impossible without the use of catheters and implants in contact with the skin, mucosal epithelium and blood of the patient. An important task in the manufacture of implantable medical devices is their resistance to protein biofouling and the formation of bacterial biofilm on their surface. The interaction of bacteria with graphene oxide particles contributed to a decrease in the number of Staphylococcus aureus (48.6±1.7% CFU) and Escherichia coli (29.7±2.1% CFU) bacteria. Incubation of Staphylococcus aureus and Escherichia coli cells with graphene oxide particles resulted in a decrease in the ability to biofilm formation on 96-Well Clear PVC Microplates. The creation of composite materials based on polyvinyl chloride and graphene may be one of the strategies for reducing biofilm formation on the surface of implantable medical devices.