Grapevine Red Blotch Disease Research Articles

Longer cluster hanging time decreases the impact of grapevine red blotch disease in Vitis vinifera L. Merlot across two seasons.

Grapevine red blotch virus (GRBV) is a recently discovered virus and a major concern for the wine industry. Prior research indicated that GRBV delays grape ripening by reducing °Brix and anthocyanin concentrations in grapes from infected vines, resulting in higher ethanol concentrations in wines made from healthy fruit compared to diseased vines, which impacted sensory properties. In this study, infected fruit (Vitis vinifera L. Merlot) was sequentially harvested (in 2016 and 2017) and chaptalized (in 2017) to ameliorate the impact of GRBV on grape and final wine composition. Chemical parameters including phenolic and volatile profiles of grapes and their subsequent wines were measured. Sensory properties were determined by descriptive analyses. Results demonstrated that GRBV decreased sugar accumulation and anthocyanin synthesis in grapes. Wines from GRBV grapes harvested at later ripening stage produced wines that were more similar chemically and sensorially to wines made from healthy fruit than to wines made from GRBV fruit harvested earlier. The longer hang time of GRBV grapes is a potential strategy to mitigate the impacts of GRBV. However, chaptalization of diseased fruit must was inefficient at increasing similarities with wines made from healthy fruit. This article is protected by copyright. All rights reserved.

Best Practices for Monitoring Visual Symptoms of Grapevine Red Blotch Disease in Black-Fruited Winegrape Cultivars

Best Practices for Monitoring Visual Symptoms of Grapevine Red Blotch Disease in Black-Fruited Winegrape Cultivars

Grapevine red blotch virus C2 and V2 are suppressors of post-transcriptional gene silencing

Grapevine red blotch virus (GRBV) is the causative agent of grapevine red blotch disease (GRBD) which is one of the major threats faced by grapevine industry in the United States. Since its initial identification in 2011, the disease has rapidly spread in the major US grape-growing regions of the Pacific Northwest, causing major economic impacts. Geminiviruses, the largest family of plant viruses, can induce and be targeted by host post-transcriptional gene-silencing (PTGS) anti-viral mechanisms. As a counter-defense mechanism, viruses have evolved viral silencing suppressor proteins to combat PTGS mechanisms and establish a successful infection in host plants. Here we provide characterization of two ORFs of GRBV, C2 and V2 as viral silencing suppressors. In Nicotiana benthamiana line 16c GFP marker plants, synergism or additive effects of C2 and V2 suppressors was observed at the mRNA level when they are expressed together transiently. Additionally, we showed there is no evidence by yeast two-hybrid of self-interaction (dimerization) of C2 or V2 proteins, and no evidence of physical interaction between these two suppressors.

Grapevine red blotch virus alters grape skin cell-wall composition impacting phenolic extractability during winemaking.

Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening. However, grape cell-wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large role in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking. Understanding the impact of GRBV infection on cell-wall metabolism is important for the development of potential mitigations strategies. In this study, high-throughput transcriptome sequencing was conducted on Vitis vinifera L. 'Merlot' grapes during ripening. The cell-wall composition, phenolic content, and phenolic extractability at two different commercial harvest points were also determined. Log fold changes indicated a strong induction in diseased grapes at harvest of several transcripts involved in cell-wall solubilization and degradation. However, these observations did not translate to changes in cell-wall composition at either harvest point in diseased grapes, potentially suggesting post-transcriptional regulation. Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest. Both pectin and pathogenesis-related proteins are known to retain phenolic compounds during winemaking. Our study corroborates this finding when the percentage extractability of flavonols in wines was significantly lower when made from GRBV-infected fruit. These results suggest GRBV alters the grape cell walls, consequently decreasing phenolic extraction during winemaking. © 2023 Society of Chemical Industry.

Comparative Diagnosis of Grapevine Red Blotch Disease by Endpoint PCR, qPCR, LAMP, and Visual Symptoms

Comparative Diagnosis of Grapevine Red Blotch Disease by Endpoint PCR, qPCR, LAMP, and Visual Symptoms

Adoption of Best Management Practices for Grapevine Leafroll and Red Blotch Diseases: A Survey of West Coast Growers

Grapevine leafroll disease (GLD) and red blotch disease (RBD) threaten the sustainability of the U.S. wine grape industry. To understand the factors influencing the adoption of disease management practices, we surveyed wine grape industry professionals in California, Oregon, and Washington ( n = 154). Economic factors were the fundamental cost of implementing management practices and the ability to sell products from diseased vines (salability). Respondents who encountered reduced salability were more likely to adopt virus testing, replace infected vines, and view these practices as economically favorable. Salability was a strong driver for adoption among Californian respondents but less so in Washington, where wineries appeared more willing to accept infected products. Respondents who had acquired technical knowledge of disease ecology were more likely to adopt management practices and to perceive them as economical. Conversely, when there was a lack of knowledge of GLD ecology, notably that mealybugs transmit the pathogen, adoption was reduced, and practices were considered less economical. Factors affecting adoption were broadly generalizable across diseases. However, knowledge of GLD ecology was more strongly associated with adoption, likely reflecting the remaining knowledge gaps in RBD related to vector ecology and field spread. An emphasis on grower knowledge acquisition and the development of economical disease management practices can improve adoption of best management practices for viral diseases of grapevine. [Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

Composition of Pinot Noir Wine from Grapevine Red Blotch Disease-Infected Vines Managed with Exogenous Abscisic Acid Applications

Grapevine red blotch disease (GRBD) has negative effects on grape development and impacts berry ripening. Abscisic acid (ABA) is a plant growth regulator involved in the initiation of berry ripening. Exogenous abscisic acid application was compared to an unsprayed control on GRBD-positive Pinot noir vines during two vintages, and the total monomeric anthocyanin, total phenolics, phenolic composition, and volatile profile were measured in wines. In addition, untargeted metabolites were profiled using high-resolution LC-MS/MS. Results showed that the wine composition varied by vintage year and was not consistent with ABA application. Wines from the ABA treatment had a lower total anthocyanin and total phenolic content in one year. The untargeted high-resolution LC-MS/MS analysis showed a higher abundance of phenolic compounds in ABA wines in 2019, but lower in 2018. The wine volatile compounds of ABA treatments varied by vintage. There were higher levels of free β-damascenone, β-ionone, nerol, and several fermentation-derived esters, acids, and alcohols in ABA wines, but these were not observed in 2019. Lower 3-isobutyl-2-methoxypyrazine (IBMP) was also observed in wines with ABA treatment in 2019. The results demonstrated that ABA application to the fruit zones did not consistently mitigate the adverse impacts of GRBD on Pinot noir wines.

Transmission of Grapevine Red Blotch Virus by Spissistilus festinus [Say, 1830] (Hemiptera: Membracidae) between Free-Living Vines and Vitis vinifera 'Cabernet Franc'.

Grapevine red blotch disease emerged within the past decade, disrupting North American vine stock production and vineyard profitability. Our understanding of how grapevine red blotch virus (GRBV), the causal agent of the disease, interacts with its Vitis hosts and insect vector, Spissistilus festinus, is limited. Here, we studied the capabilities of S. festinus to transmit GRBV from and to free-living vines, identified as first-generation hybrids of V. californica and V. vinifera ‘Sauvignon blanc’ (Vcal hybrids), and to and from V. vinifera ‘Cabernet franc’ (Vvin Cf) vines. The transmission rate of GRBV was high from infected Vcal hybrid vines to healthy Vcal hybrid vines (77%, 10 of 13) and from infected Vvin Cf vines to healthy Vcal hybrid vines (100%, 3 of 3). In contrast, the transmission rate of GRBV was low from infected Vcal hybrid vines to healthy Vvin Cf vines (15%, 2 of 13), and from infected Vvin Cf vines to healthy Vvin Cf vines (19%, 5 of 27). No association was found between transmission rates and GRBV titer in donor vines used in transmission assays, but the virus titer was higher in the recipient leaves of Vcal hybrid vines compared with recipient leaves of Vvin Cf vines. The transmission of GRBV from infected Vcal hybrid vines was also determined to be trans-stadial. Altogether, our findings revealed that free-living vines can be a source for the GRBV inoculum that is transmissible by S. festinus to other free-living vines and a wine grape cultivar, illustrating the interconnected roles of the two virus hosts in riparian areas and commercial vineyards, respectively, for virus spread. These new insights into red blotch disease epidemiology will inform the implementation of disease management strategies.

Identification of Potential Grapevine Red Blotch Virus Vector in Missouri Vineyards

Grapevine red blotch virus (GRBV), the causal agent of grapevine red blotch disease, has been detected in vineyards across the United States and throughout Missouri. Insect transmission of GRBV in cultivated vineyards of Missouri has not been investigated previously. The objectives of this study were to characterize the potential insect vectors present in four commercial vineyards that had previously been determined to be infected with GRBV, test potential vectors caught in vineyards and surrounding habitats for the presence of GRBV with the use of PCR, and investigate the ability of candidate vectors to acquire and transmit GRBV using controlled greenhouse experiments. Of the vineyard-collected insects tested over the course of this research, one species of treehopper, <i>Entylia carinata</i>, tested positive for GRBV. This species and one other treehopper, <i>Enchenopa binotata</i>, were selected for direct transmission assays. Both species successfully acquired GRBV from infected grapevines and transmitted GRBV to confirmed GRBV-free grapevines. <i>E. carinata</i> has been identified as a promising economic vector after insect samples from vineyards tested positive for GRBV, and monitoring data placed this species as the second-most abundant treehopper captured in traps. We do not consider <i>E. binotata</i> a likely economically significant vector because our monitoring data showed that this species was rare and only found along edge habitat surrounding vineyards, never inside vineyard rows. Samples of the most abundant treehopper, <i>Micrutalis calva</i>, have not tested positive, but its vector status remains unresolved. Further research on rates of secondary spread and transmission by <i>M. calva</i> are required, but these results provide evidence that insect transmission of GRBV is feasible in the region.

Epidemiology of Grapevine Red Blotch Disease Progression in Southern Oregon Vineyards

Grapevine red blotch disease (GRBD) is an emerging disease caused by grapevine red blotch virus (GRBV). It is widespread in most United States winegrape production regions and differential spread dynamics have been reported between the regions. This study surveyed eight vineyard sites in southern Oregon over four years for the progression of GRBD incidence. The vineyards included five sites that were five years or older and three sites that were three-years-old. The disease incidence in the older blocks ranged from 2.81 to 58.78%, while in the younger blocks it ranged from 0.29 to 1.11%. Some vineyards implemented frequent disease scouting, removing infected vines and replanting. The disease incidence in these blocks remained &lt;5% over the survey period. However, in vineyards with no roguing and replanting, the disease incidence increased nearly 30-fold after three years. We analyzed the spatial distribution of the disease in vineyards surveyed in 2020 and found that the disease distribution is highly aggregated based on Spatial Analysis by Distance Indices (SADIE). In a separate study, we also tested the GRBV infection status of asymptomatic vines next to symptomatic vines to inform decision-making when roguing and replanting. Out of 410 asymptomatic vines surrounding 41 symptomatic vines, only two tested positive for GRBV. Additionally, in 2020 and 2021, we tested the GRBV status of previously identified possible alternative host species: blackberries (<i>Rubus armeniacus</i>) and wild/feral grapes (<i>Vitis</i><i>riparia</i>) collected from areas surrounding the four survey sites. GRBV was present in 10 to 70% of wild grape samples in both years and in 10% of the blackberry samples in 2020. However, the virus titer was low in blackberry samples and it was not detected in 2021 samples. These results indicate the potential importance of wild grapes as alternative hosts on GRBD incidence and spread in southern Oregon vineyards, while blackberry is unlikely to be an alternative host with epidemiological significance.

Survey of Vineyard Insects and Plants to Identify Potential Insect Vectors and Noncrop Reservoirs of Grapevine Red Blotch Virus

Grapevine red blotch virus (GRBV) is a DNA virus in the family Geminiviridae. This pathogen is the causal agent of grapevine red blotch disease, which affects cultivated grapevines and leads to negative effects on crop quality and yield. GRBV is present in commercial vineyards across North America, indicating that spread might have been largely human mediated. That said, recent surveys have demonstrated that there appears to be secondary transmission, most likely by an insect vector. In this study, vineyard insects and plants were surveyed to identify potential candidate vectors and noncrop plants that could act as reservoirs for this pathogen. Results reconfirm that GRBV is limited to Vitis spp., including both cultivated and wild grapevines. Eleven insect genera or species, field collected in vineyards, tested positive for GRBV using quantitative polymerase chain reaction. These insect taxa include unknown Aphididae, the cicadellids Aceratagallia spp., Acinopterus angulatus, Caladonus coquilleti, Colladonus montanus reductus, Colladonus sp., Empoasca spp., Erythroneura elegantula, and Scaphytopius graneticus, along with the membracid Spissistilus festinus and an unknown delphacid. Of these organisms, S. festinus is already known to be capable of transmitting GRBV, and Scaphytopius graneticus may merit closer evaluation as a candidate vector. [Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

Occurrence of Grapevine Red Blotch Virus in Wine Grapes in Arizona

During August to October 2021, symptoms of leaf reddening similar to those associated with grapevine red blotch disease were observed on several red variety blocks in a vineyard located in the Willcox area of Cochise County, Arizona. To our knowledge, this is the first report of grapevine red blotch virus in Arizona vineyards.

Modifications of Grapevine Berry Composition Induced by Main Viral and Fungal Pathogens in a Climate Change Scenario.

The grapevine is subject to high number of fungal and viral diseases, which are responsible for important economic losses in the global wine sector every year. These pathogens deteriorate grapevine berry quality either directly via the modulation of fruit metabolic pathways and the production of endogenous compounds associated with bad taste and/or flavor, or indirectly via their impact on vine physiology. The most common and devastating fungal diseases in viticulture are gray mold, downy mildew (DM), and powdery mildew (PM), caused, respectively by Botrytis cinerea, Plasmopara viticola, and Erysiphe necator. Whereas B. cinerea mainly infects and deteriorates the ripening fruit directly, deteriorations by DM and PM are mostly indirect via a reduction of photosynthetic leaf area. Nevertheless, mildews can also infect berries at certain developmental stages and directly alter fruit quality via the biosynthesis of unpleasant flavor compounds that impair ultimate wine quality. The grapevine is furthermore host of a wide range of viruses that reduce vine longevity, productivity and berry quality in different ways. The most widespread virus-related diseases, that are known nowadays, are Grapevine Leafroll Disease (GLRD), Grapevine Fanleaf Disease (GFLD), and the more recently characterized grapevine red blotch disease (GRBD). Future climatic conditions are creating a more favorable environment for the proliferation of most virus-insect vectors, so the spread of virus-related diseases is expected to increase in most wine-growing regions. However, the impact of climate change on the evolution of fungal disease pressure will be variable and depending on region and pathogen, with mildews remaining certainly the major phytosanitary threat in most regions because their development rate is to a large extent temperature-driven. This paper aims to provide a review of published literature on most important grapevine fungal and viral pathogens and their impact on grape berry physiology and quality. Our overview of the published literature highlights gaps in our understanding of plant-pathogen interactions, which are valuable for conceiving future research programs dealing with the different pathogens and their impacts on grapevine berry quality and metabolism.

Grapevine Red Blotch Disease Etiology and Its Impact on Grapevine Physiology and Berry and Wine Composition

Grapevine red blotch virus (GRBV) has become widespread in the United States since its identification in 2012. GRBV is the causative agent of grapevine red blotch disease (GRBD), which has caused detrimental economic impacts to the grape and wine industry. Understanding viral function, plant–pathogen interactions, and the effects of GRBV on grapevine performance remains essential to developing potential mitigation strategies. This comprehensive review examines the current body of knowledge regarding GRBV, to highlight gaps in the knowledge and potential mitigation strategies for grape growers and winemakers.

Impact of Rootstock and Season on Red Blotch Disease Expression in Cabernet Sauvignon (V. vinifera).

Grapevine red blotch virus (GRBV), the causative agent of grapevine red blotch disease, is widespread across the United States and causes a delay in ripening events in grapes. This study evaluates the effects of GRBV on Cabernet Sauvignon grape berry composition, grafted on two different rootstocks (110R and 420A) in two seasons (2016 and 2017). Total soluble solids, acidity, and anthocyanin concentrations were monitored through ripening and at harvest. Phenolic and volatile compounds were also analyzed at harvest to determine genotypic and environmental influences on disease outcome. Sugar accumulation through ripening was lower in diseased fruit (RB (+)) than healthy fruit across rootstock and season. GRBV impact was larger in 2016 than 2017, indicating a seasonal effect on disease expression. In general, anthocyanin levels and volatile compound accumulation was lower in RB (+) fruit than healthy fruit. Total phenolic composition and tannin content was higher in RB (+) fruit than healthy fruit in only 110R rootstock. Overall, GRBV impacted Cabernet Sauvignon grape composition crafted on rootstock 110R more than those crafted on rootstock 420A.

Use of Ground Covers to Control Three-Cornered Alfalfa Hopper, Spissistilus festinus (Hemiptera: Membracidae), and Other Suspected Vectors of Grapevine Red Blotch Virus

Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease, which affects wine grapes and leads to reduced crop yield and quality. The pathogen-plant-vector relationship of GRBV is not well understood; however, some possible vectors have been identified: Caladonus coquilletti (Van Duzee; Hemiptera: Cicadellidae), Colladonus reductus (Van Duzee; Hemiptera: Cicadellidae), Erythroneura spp., Melanoliarus sp. (Hemiptera: Cixiidae), Osbornellus borealis DeLong. & Mohr (Hemiptera: Cicadellidae), Scaphytopius granticus (Ball; Hemiptera: Cicadellidae), Spissistilus festinus (Say). Of these species, S. festinus has been shown to transmit the virus to uninfected grapevines, making it of particular interest. Since the pathogen-plant-vector relationship of GRBV is not yet completely understood, pesticide use is not necessarily the best way to manage these possible vectors. Here we test if ground cover removal, by discing in spring, could reduce the activity of potential GRBV vectors. We show that S. festinus presence in the canopy was reduced in disc rows compared to just mowing the ground vegetation, whereas there were no differences in presence in the canopy between disc and mow rows of the other possible insect vectors. Erythroneura elegantula (Osborn; Hemiptera: Cicadellidae), a common pest of grapevines but not a candidate GRBV vector, was found to have higher densities in the canopy in disc rows compared to mow rows, an effect possibly mediated by changes in vine vigor associated with ground covers. We conclude that if S. festinus is a primary vector of GRBV, discing ground covers in early spring may be a viable way to reduce their presence in the vine canopy.

Impact of grapevine red blotch disease on primary and secondary metabolites in ‘Cabernet Sauvignon’ grape tissues

The grapevine red blotch disease (GRBD) was first noticed in 2008, impacting grape ripening. In general, GRBD reduces grape and wine quality resulting in significant economic losses. The purpose of the present study was to evaluate the effect of GRBD on agronomical parameters of ‘Cabernet Sauvignon’ vines at harvest. Using a metabolomics approach, the influence on primary and secondary metabolite profiling in skin + pulp/flesh and seeds were also determined. GRBD influenced °Brix and berry weight, as well as primary and secondary metabolites in both tissues. 1D 1H NMR was effective in quantifying the main primary and secondary metabolites affected by GRBD. RP-HPLC was similarly able to quantify the main phenolics affected. Multivariate analysis showed the influence of the virus on grape metabolites using both tools in two berry tissues. The effectiveness of both tools to describe sample variability was compared and the most affected metabolites in each tissue could be identified.

Timing the Implementation of Cultural Practices for Spissistilus festinus (Hemiptera: Membracidae) in California Vineyards Using a Stage-Structured Degree-Day Model.

The three-cornered alfalfa hopper, Spissistilus festinus (Say), was shown to transmit Grapevine red blotch virus (GRBV), the causative agent for Grapevine red blotch disease, in a greenhouse study on grapes. GRBV is a major concern of wine grape growers due to its economic impact on wine quality. Plants in the family Fabaceae are preferred hosts of S. festinus and are commonly planted as cover crops or present in a vineyard's native vegetation. In late winter, during grapevine dormancy, S. festinus migrate into vineyards to feed and reproduce on these cover crop and weed hosts. Tilling vineyard floor vegetation provides growers an opportunity to disrupt the life cycle of early instars that are relatively immobile, reducing the S. festinus first-generation population. Nymphal presence is difficult to detect. First through third instars were not detected in sweep net samples in a 2-yr weekly sampling study, whereas fourth and fifth instars were first found on the same sample date as emerging adults. A degree-day model was developed and successfully predicted when early S. festinus instars are present in the vineyard to aid in exploiting the time period when S. festinus is most susceptible to cultural control measures.

Influence of Riparian Habitat and Ground Covers on Threecornered Alfalfa Hopper (Hemiptera: Membracidae) Populations in Vineyards.

Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease, which affects wine grapes and leads to reduced crop yield and quality. While some virus spread can be attributed to the propagation of infected plant material, a greenhouse assay recently demonstrated that the threecornered alfalfa hopper (Membracidae: Spissistilus festinus Say) can transmit GRBV between grapevines. While S. festinus is not considered an economic pest of wine grapes, this species is present in California vineyards and their feeding can cause petiole girdling. Recent surveys have noted a correlation between S. festinus populations and GRBV-positive vines in vineyard areas adjacent to riparian habitat. Here, S. festinus populations were monitored over a 2-yr period at multiple vineyard sites adjacent to riparian habitats. At each site, insects were sampled from ground covers and the vine canopy at the vineyard edge and interior, and vines in both locations were evaluated for petiole girdling. Results indicate that there was no difference in abundance of S. festinus at the vineyard edge and interior. Populations in the vine canopy were highest in the late spring and early summer, and this was followed by the appearance of petiole girdling, indicating a key period of potential GRBV transmission. Furthermore, activity in the vine canopy appears to be amplified when the quality of ground covers is reduced as the season progresses. That said, overall populations of S. festinus were relatively low and additional work is needed to characterize the timing and efficiency of transmission under field conditions.

Impact of Grapevine Red Blotch Disease on Cabernet Sauvignon and Merlot Wine Composition and Sensory Attributes

Grapevine red blotch disease (GRBD) is a recently identified viral disease that affects grapevines. GRBD has been shown to impact grapevine physiology and grape composition by altering specific ripening events. However, no studies have been reported on the impact of GRBD on wine composition and its sensory attributes. This study evaluated the impact of GRBD on wine primary and secondary metabolites, in addition to its sensory properties, when making wines from Cabernet Sauvignon and Merlot grapes during two seasons. Wines made with GRBD-impacted fruit were lower in ethanol content when compared to wines made with grapes from healthy grapevines. This was attributed to the lower total soluble sugar (TSS) levels of diseased grapes due to delayed ripening at harvest. GRBD impacted wine phenolic composition by decreasing anthocyanin concentrations and increasing flavonol concentrations in some instances. Additionally, proanthocyanidin concentrations were also consistently higher in GRBD wines compared to wines made from healthy fruit. Descriptive analysis demonstrated that GRBD can impact wine style by altering aroma, flavor, and mouthfeel attributes. However, the extent of GRBD impact on wine composition and sensory properties were site and season dependent.