Grape Varieties Research Articles

Development of a technology of manufacturing Extra Virgin oil from seeds of different grape cultivars

It has been studied whether seeds of the grape varieties Lydia, Isabella, Moldova, Baco, Odesa Black, Cabernet, and the blends N 1 – Muscat Blanc + Noah (50:50), N 2 – Riesling + Chardonnay (50:50), and N 3 – Chardonnay + Sukholimansky (50:50) are promising raw materials for fat-and-oil products and cosmetics. The study provides a scientific basis for a technology for manufacturing oils from several grape seed varieties. For seeds from different grape cultivars, appropriate process conditions have been selected to obtain extra virgin oil by cold pressing. The most practical conditions for wet-heat treatment of crushed seeds of the cultivars Cabernet, Moldova, Baco, Odesa Black, and blend N 3 (Chardonnay + Sukholimansky, 50:50) are a temperature of 40 °C and a duration of 10 min. In contrast, seeds of Lydia, Isabella, and blends No. 1 (Muscat Blanc + Noah, 50:50) and N 2 (Riesling + Chardonnay, 50:50) should be treated at the same temperature for 20 min. While extracting oil by pressing, it is recommended to avoid heating the treated crushed seeds (extraction mash) to over 30 °C for more than 5 min. The research results have shown that in the seeds of Lydia, Isabella, Moldova, Baco, Odesa Black, and Cabernet grapes, and those of blends N 1 (Muscat Blanc + Noah, 50:50), N 2 (Riesling + Chardonnay, 50:50), and N 3 (Chardonnay + Sukholimansky, 50:50), the oil content is 14–20 %.

Study on Quality of Verjuice Affected by Grape Types, Treatments and Storage at Ambient Conditions

To improve the quality of grape berries and bunches, berry thinning is adopted. In this practice, a sizeable quantity of thinned berries is destroyed despite having valuable contents. Thinned berries are utilized to produce verjuice in different countries. To assess the effect of grape varieties and ingredients on verjuice quality, the present study was conducted at ICAR-National Research Centre for Grapes, Pune during the fruiting season of 2022-2023. Thinned grape berries (6-8 mm diameter) from four varieties namely Manjari Naveen, Crimson Seedless, Thompson Seedless and Manjari Kishmish were utilized with three treatments as the addition of 0.49 g NaCl salt/100 mL, 3 mL olive oil/100 mL and combination of 0.49 g NaCl salt and 3 mL olive oil/100 mL. Physico-chemical parameters of prepared verjuice types were observed initially as well as 15, 30 and 45 days of storage at ambient conditions. At the initial stage, among the varieties, treatments and their interactions showed non-significant differences for TSS, acidity and pH. Among the different verjuice types, except Crimson Seedless with salt and olive oil, were recorded with maximum TSS. The pH values Were ranged from 2.35 to 2.57 while the maximum recorded titratable acidity was 3.07% whereas the lowest acidity was 2.43%. Verjuice storage at ambient conditions resulted in reduced TSS, acidity and pH. Verjuice prepared from Manjari Kishmish showed superiority in all sensory parameters while verjuice from Thompson Seedless was scored minimum. Results recorded in the present study revealed that the quality of verjuice was acceptable and need to study the combinations of different type grapes to improve verjuice quality and pesticide degradation in the process.

The Detection of Crown Gall Disease in Kahramanmaraş and Surrounding Provinces

Aims: The aim of the study is to isolate the bacterial agent causing crown gall disease in Kahramanmaraş and its surrounding provinces, determine its prevalence in the region, and conduct its identification. 
 Material and methods: Between June and September of 2017-2019, strains obtained from the shoots and galls of vines showing symptoms of galls in vineyards in Kahramanmaraş, Adıyaman, Kilis, Gaziantep, and Şanlıurfa provinces were subjected to pathogenicity tests, classical, molecular, and MALDI-TOF MS identification.
 Results and Discussion: In this study, the prevalence of crown gall disease in 46 vineyards showing disease symptoms was determined as follows: Kahramanmaraş 76.47%, Adıyaman 75%, Gaziantep 50%, Kilis 33.33%, and Şanlıurfa 20%. From the 46 contaminated vineyards in the study area, 24 pathogenic of R. vitis strains were obtained. In the pathogenicity test conducted with R. vitis strains on the Hatun Parmağı grape variety under greenhouse conditions, the most virulent strains were determined based on the weights of the galls formed, and they were identified as CU52-3/2, CU39-11/5, and CU39-8/1. According to classical tests, 13 strains showed 100% similarity to R. vitis, while molecular characterization with R. vitis-specific primers revealed that 17 strains had the virA gene, 13 strains had the pehA gene, 10 strsains had the virF gene, and 6 strains had the octopine-type plasmid. Ten strains, representing the sample, were identicated as R. vitis through MALDI-TOF MS analysis. This study revealed the impact of pathogenic strains with different virulences on the same grape variety. In line with this, of control methods against highly virulent bacterial agents in reducing losses in plants can be investigated for their applicability in field and orchard areas in subsequent studies.
 Conclusions: Research on different grape varieties is essential to develop varieties that are resistant or tolerant to R. vitis, the pathogen causing this prevalent disease in Turkish viticulture.

Hybrid variety Dionis (Cabernet Franc E11 × Panonia) vs. Cabernet Franc wines: Comparative study of chemical profile, in vitro biological activity, sensory quality and potential health risk

In the 20th century, resistant grape varieties did not succeed due to the lack of wine quality analyses prior to introduction to the market. Thus, newly created resistant varieties should be tested for wine quality characteristics. This study evaluates new, resistant interspecies variety Dionis and parental Cabernet Franc wines (vintages 2017−2021): chemical characteristics (oenological parameters; polyphenolic profile by HPLC analysis), in vitro biological activity (biochemical and cell-based assays related to enzyme inhibitory, antioxidant and anti-inflammatory activity), safety aspects (ochratoxin A (OTA) occurrence), and sensory profile. According to oenological parameters, Dionis wine is very similar to Cabernet Franc wine. The most abundant phenolic acid in all samples was gallic acid (14.77−99.08 mg/L), catechin (8.12–41.09 mg/L) was the dominant flavonoid, and malvidin-3-O-glucoside (1.5–120.8 mg/L) the leading anthocyanin. Some differences in polyphenolic profile arose due to variety; annual environmental factors probably do not have great impact. All samples expressed considerable in vitro biological activity, reasonably correlated with content of some polyphenolics, such as anthocyanins. OTA was not detected in any sample. In general, sensory properties of Dionis and Cabernet Franc were similar, while, dependent on vintage, Dionis showed a more variable sensory profile.

Phenological Evaluation of Minority Grape Varieties in the Wine Region of Madrid as a Strategy for Adaptation to Climate Change

In this study, a total of 34 Spanish minority varieties were studied during four seasons from 2020 to 2023, and their behavior was characterized according to their main phenological stages (bud break, bloom, veraison, and maturity) and complete cycle. We focused on the varieties prospected in the central Spanish plateau and conserved in the “El Encín” grapevine collection, aiming to identify the potential for adaptation of these varieties and for them to be considered by winegrowers as an alternative to current climate change conditions. The growing degree days required for the expression of the phenological stage, and the duration of each stage, were compiled. Characteristics of oenological interest were also monitored, such as °Brix, pH, and titratable acidity in must at the time of harvest. This study was carried out in years with atypical snowfall and cold spells (winter 2021), as well as with heat waves (summer 2022), with average temperatures 3–5 °C higher than normal and absolute maximum temperatures over 40 °C. Both cases also exceeded records of historical series (1957–2019). Veraison has been identified as the stage most susceptible to damage from high temperatures, in addition to the maturation and duration of the complete cycle. The varieties were classified into five groups according to the duration of each phenological stage (very early, early, medium, late, and very late). Some varieties with late or very late maturation and with must characteristics of 20–23 °Brix, 3–5 g/L of titratable acidity, and pH 3.5–4.5, as well as others, retained stability in their phenological periodicity and must quality. The results suggest that special attention should be given to thoroughly evaluating these varieties, and that the strategies contemplated should be considered as a feasible cultivation alternative in viticulture to mitigate the effects of climate change.

Multi-nutrient fertilization-based analysis of fruit quality and mineral element composition during fruit development in Merlot wine grapevine1

Mineral elements play a crucial role in plant growth and development. Ensuring the proper supply of these elements in soil and maintaining an optimum range within plants is necessary for achieving optimal fruit yield and quality. Unfortunately, the application of NPKCaMg fertilizers to fruit trees is often either insufficient or excessive, leading to both environmental degradation and reduced fruit yield and quality. To assess the impact of different fertilizers on the biological-related traits of grape fruit and its response to nutritional elements, Merlot grapevines were subjected to multi-nutrient fertilization over four consecutive growing seasons from 2018 to 2021 in Penglai District, Yantai, China. Principal component analysis revealed that T11 treatment, consisting of N3P3K1Ca2Mg4, was the most suitable fertilizer type and application design for this experiment. The application of T11 resulted in a significant decrease (24.29%–35.20%) in fertilizer usage, and it resulted in an increase in several important traits such as 100 grain weight (HGW), number of seeds (SN), total soluble solids (TSS), total seed phenols (SP), seed flavanols (SFI), and seed tannins (ST) by 3.28%–12.84%, 3.76%–20.03%, 1.11%–14.95%, 2.16%–23.69%, 11.00%–32.78%, 1.07%–23.35%, respectively, compared to T14 (N4P2K3Ca1Mg4), T16 (N4P4K1Ca3Mg2), T13 (N4P1K4Ca2Mg3), and T15 (N4P3K2Ca4Mg1) treatments. Flowering and fruiting behavior exhibited a considerable demand for NPK, with a higher requirement for K and B during fruit growth and development compared to the other mineral elements. Excessive K in soil was found to enhance the competitive inhibition of Ca uptake by Merlot grapevines. The optimum ranges of mineral element content for total peel phenols (PP), peel flavanols (PFI), total peel flavonoids (PFD), total seed phenols (SP), and seed tannins (ST) were primarily influenced by grape variety and nutritional analysis method. In conclusion, the careful selection of NPKCaMg fertilizer and its meticulous application to soil at an optimum range of mineral elements are critical for grapevine growth and development.

Morphological, molecular biology and pathological characterization of fungi causes root rot on grapevine

Background Grapevine is subjected to attack by several of soilborne fungi causing root-rot diseases that lead to limit the production of grapevine. Objective This study aimed to investigate the morphological, molecular identification, and pathogenic potential of fungi that causes root-rot disease of grapevine in Egypt. Materials and methods Isolation of fungi causing root rot of grapevine plants from the samples of diseased roots of different cultivars, i.e., crimson, superior, and flame seedless. Identification according to characterizations of morphological, cultural, and molecular biology based on internal-transcribed spacer 1 (ITS1). Pathogenicity tests of fungal isolates on grapevine plants under greenhouse conditions. Root-rot incidence, disease severity, and plant growth characteristics were determined. Results and conclusion Isolation traits from affected grapevine trees with different grape varieties in Egypt’s El-Nobaria Province, El-Behira Governorate, yielded eighteen isolates of four fungal genera. Fusarium spp. was the most fungal genus highly frequent on all cultivars tested and colonization (100%) on root-rot tissue of superior and flame-seedless cultivars, then (75%) on Crimson cultivar. While Lasiodiplodia spp. was recorded with moderate frequency and high colonization (100%) on rotten tissue of Crimson cultivar followed by (75%) in both flame-seedless and superior cultivars. As opposed to that, fungi of Macrophomina phaseolina followed by Rhizoctonia solani had the least frequency and colonization percentage. Isolates of fungi were identified according to morphological characteristics, cultural, and molecular biology based on internal-transcribed spacer-1 (ITS1) sequencing and conserved in GenBank with accession numbers from (ON037457.1 to ON037474.1). Fungal isolates were varied for causes of root rot on grapevine plants and their reduction effect on the characteristics of growth of flame-seedless cultivar. Fusarium solani isolate (ON037462.1) was the most pathogenic isolate that caused (100%) of root rot, high disease severity, and highly significant reduced grapevine plant growth characteristics followed by isolates of Lasiodiplodia theobromae (ON037474.1). In this respect, a new isolate of fungal species was hosting grapevine plants, i.e., two isolates each of F. chlamydosporum, F. brachygibbosum, one isolate of F. ipomoeae, and one isolate of L. exigua are the new causal pathogens of root-rot disease on grapevine as the first report in Egypt.

Authentication of selected white wines by geographical origin using ICP spectrometric and chemometric analysis

An important aspect of assessing the authenticity of wines is its geographical origin. The aim of the work is to authenticate by geographical origin according to the data of the ICP-spectrometric and chemometric analysis of elemental "images" of wines produced from white grape varieties Chardonnay, Riesling and Muscat grown in four regions of the Krasnodar Territory, Russia. The difference in the contents of Al, Ba, Ca and Rb in wines was found depending on the variety, and Al, Ba, Rb, Fe, Li, Sr – depending on the region of grape growth. Different models of the experimental data processing were used for attribution of the produced varieties of wine to the area of the grape's growth. The criterion for the quality of the constructed models was the accuracy of the attribution of a wine variety to the area of the grape's growth (%). Analysis of the elemental analysis data of 153 wine samples showed that in terms of attribution accuracy, automated neural networks (100 %) are preferred among machine learning methods, followed by support vector machines (98.69 %) and general discriminant analysis (94.77 %). The applied mathematical models enabled the revealing of the cluster structure of the analyzed wine varieties and their attribution to the area of a grape growth with high accuracy. Sr, Li and Fe concentrations in wines were found as the dominating predictors in the constructed models for definition of the geographical origin of wines. The combination of ICP-spectrometric analysis data with the capabilities of statistical modeling of machine learning methods focused on large-dimensional data made it possible to successfully solve small-dimensional problems of the definition of the geographical origin of wines by their elemental composition and variety.

EXPLORING THE MICRO AND MACRO TERROIR OF FETEASCĂ NEAGRĂ WINE FROM MOLDOVA

This study delves into the intricate realm of Moldovan winemaking, focusing on the iconic Fetească Neagră grape variety. The research highlights the importance of indigenous grape varieties in shaping a unique wine identity for the region. Drawing on the concept of terroir, the study investigates the microbial terroir specific to three designated geographical regions in Moldova during the 2022 vintage. The research employs a multifaceted approach, combining climate data analysis, mini vinification experiments, microbial DNA extraction, and sensory evaluation techniques. Results reveal significant variations in climatic conditions and microbial diversity across the three regions, influencing the sensory characteristics of the resulting wines. On vintage 2022 the Codru is temperature， and the Stefan-Voda and Vadul lui Traian are warm climates. All the 3 regions are moderately dry. The Stefan Voda is 14.0%， and manifests the highest ethanol content among the regions, alongside the lowest pH level. The sensory evaluations show Stefan Voda evincing heightened creaminess, Codru showcasing prominent herbal nuances, and Vadul lui Traian displaying elevated scores in berry, cherry, violet, and black pepper descriptors. Through meticulous analysis, the study underscores the dynamic interplay between environmental factors, microbial communities, and wine attributes, shedding light on the complex tapestry of Moldovan winemaking. Ultimately, this research contributes to a deeper understanding of terroir-driven wine production and emphasizes the importance of preserving indigenous grape varieties to uphold the distinctiveness of Moldovan wines on the global stage.

Виноградарство и виноделие в Дагестане (XVIII – начало ХХ в.): традиционные технологии и аграрная обрядность

Целью исследования является выявление зональных особенностей возделывания винограда и его переработки, а также анализ традиционных обрядов, связанных с виноградарством и виноделием. Использованы архивные источники, опубликованные законодательные акты и литературные сведения. Определены способы выращивания винограда, религиозно-магические представления и аграрные культы, связанные со сбором урожая и традициями изготовления домашнего вина. Выявлены правовые коллизии, запрещающие употребление крепких напитков. Установлено, что разведение винограда в Дагестане имеет зональные особенности: в горах виноград размножали саженцами, прививками, черенками и отводками, на равнине – на деревьях, кольях, в расстил и лежачей лозой, а также аллейным и проволочным методами, привнесенными армянскими и грузинскими виноделами. Статья является первым исследованием виноградарства и виноделия на региональном уровне, предпринятым с позиции этнографической науки. The aim of the study is to identify zonal characteristics of grape cultivation and processing, as well as to analyze traditional rituals associated with viticulture and winemaking. Archival materials; statistical data; testimonies of Russian officers and travelers of the 19th century; works of historians, ethnographers and specialists in the wine industry were studied. The methodology is based on the use of the historical-comparative method and the method of historical reconstruction. Using these methods, endemic grape varieties and traditional methods of cultivating them were identified, ancient recipes for making homemade wine were restored, and agricultural rituals associated with harvesting and traditions of making homemade wine were described. The role of terrace farming is emphasized as one of the main factors in the development of viticulture in the mountain-valley zone of Dagestan. Legal conflicts resulting from the ban on the consumption of strong drinks were identified. It was established that endemic grape varieties were grown in the mountains for their own consumption, wine production and its exchange for agricultural and livestock products; There were two types of wine produced: boiled and unboiled. The main centers of winemaking in Dagestan were identified, located in the mountain-valley zone (the villages Gimry, Zubutli, Gotsatl, etc.), on the plain (the city Kizlyar and its environs) and in Southern Dagestan (the city Derbent). The history of the formation of wine production in these urban centers was analyzed. As the study showed, the main reasons for the decline of viticulture in the mountains were land shortages and religious prohibitions related to the consumption of wine. Local rulers at the legislative level introduced a ban on the consumption, production and sale of wine; the ban on the use of alcohol was often part of the local system of customary law (the so-called adats). It was established that the cultivation of grapes in Dagestan had zonal features: in the mountains, grapes were propagated by seedlings, grafting, cuttings and layering; on the plain, on trees, stakes, spread and lying vines, alley and wire systems introduced by Armenian and Georgian winemakers. In addition, the cultivation of grapes in the region depended on natural and climatic conditions – endemic varieties were grown in the mountains, imported varieties were grown on the plains. The work is the first study of viticulture and winemaking from the perspective of ethnographic science, and is intended to revive interest in the autochthonous varieties of Dagestan grapes and their cultivation, taking into account zonal characteristics.

Varietal suitability of grapes in different agro-climatic regions of India

Grape cultivation, in India, is dependent on a narrow range of table grape varieties, with more than 70 per cent cultivated area in Maharashtra alone, followed by Karnataka and Tamil Nadu. Therefore, to increase the scope of area extension under grape cultivation, the suitability of six coloured grape varieties was assessed for yield, quality and phenological parameters at four locations, i.e., Pune (Maharashtra), Arabhavi (Karnataka), Rajendranagar (Telangana) and Theni (Tamil Nadu) during 2019-2022. From the study, it was revealed that the ‘Manjari Shyama’ and ‘Red Globe’ yielded the maximum (13.7 kg/vine) as compared to the rest of the varieties. Variety ‘Manjari Shyama’ was found stable at all four locations for yield, bunch weight, 100-berry weight, berry diameter, TSS, acidity, sugar: acid ratio and days to veraison with the regression coefficient 1.22, 1.11, 1.01, 1.13, 1.35, 1.44, 0.88 and 0.79, respectively. The yield and bunch weight in ‘Red Globe’ was found to be more expressive under the favourable environment as compared to ‘Fantasy Seedless’ (5.59 kg/vine), ‘Nanasaheb Purple Seedless’ (6.13 kg/vine) and ‘Crimson Seedless’ (7.63 kg/vine), which were found lower.

The Use of Hanseniaspora opuntiae to Improve 'Sideritis' Wine Quality, a Late-Ripening Greek Grape Variety.

In view of climate change and the increasingly antagonistic wine market, the exploitation of native genetic resources is revisited in relation to sustainable wine production. 'Sideritis' is a late-ripening Greek grape variety, which is quite promising for counteracting wine quality issues associated with the annual temperature rise. The aim of this study was to improve the quality and to enhance the aroma of 'Sideritis' wine through the use of native yeasts. To improve vinification, Hanseniaspora opuntiae L1 was used along with Saccharomyces cerevisiae W7 in mixed fermentations (SQ). The addition of H. οpuntiae significantly altered the chemical profile of the wine compared to the single-inoculated fermentations with W7 (IS). H. opuntiae increased all the acetate esters, except for hexyl acetate and (Z)-3-hexen-1-ol acetate. The concentration of 2-phenylethyl acetate, which imparts flowery and sweet notes, exhibited a 2.6-fold increase in SQ as compared to IS wines. SQ also showed higher levels in several ethyl esters, including ethyl butyrate, ethyl heptanoate and ethyl 7-octenoate, which are associated with fruity notes compared to IS. H. opuntiae produced citronellol, a terpene associated with rose and green notes, and increased the overall acceptance of the wine. Present results are thus quite promising for improving 'Sideritis' wine quality towards a sustainable wine production in Greece in view of global warming.

ЭНОКАРПОЛОГИЧЕСКОЕ ИССЛЕДОВАНИЕ ГРОЗДЕЙ И ЯГОД НЕКОТОРЫХ АБОРИГЕННЫХ И ИНТРОДУЦИРОВАННЫХ СТОЛОВЫХ И ТЕХНИЧЕСКИХ СОРТОВ ВИНОГРАДА, ВЫРАЩИВАЕМЫХ В УСЛОВИЯХ АБШЕРОНА

The article presents the results of studying the enocarpological characteristics of bunches and berries of local and introduced table and wine grape varieties cultivated in the Absheron region. In the course of the studies, it was found that indicators such as bunch weight, stem weight, skin and pulp residue, juice yield, and the amount of solid residue varied over a wide range depending on the grape variety; in particular, large, medium and small bunches were observed. During the research, it was found that such table and technical grape varieties as Tuya-tish, wild form 2, Alphonse Lavallee, Nagshebi, Digah kechimemesi, Gyozel uzum, Ganjavi, Digah gorasy, Ag Kharji, Uzun salkhym, according to enocarpological indicators (bunch weight, stem weight, skin and pulp residue, juice yield, percentage of seeds in the berry, weight of 100 seeds, solid residue) were noticeably different from other varieties. In the studied table grape varieties, the enocarpological parameters varied over wide ranges, the average weight of the bunch was 210.0-974.0 g, the weight of the stem concerning the total weight of the bunch was 0.7-9.6%, the residue of the skin and pulp was 18.0-40.0 %, juice yield – 51.0-72.7 %, seeds – 2.4-10.8 %, amount of solid residue – 27.3-49 %, and weight of 100 seeds – 2.7-14.0 g. In wine grape varieties, these indicators differed from table varieties. They were estimated as follows: bunch weight 178.7-548.0 g, stem weight concerning the total weight of the bunch – 1.8-6.5 %, the skin and pulp residue – 14.7-31.6 %, juice yield – 64.1-76.2 %, seeds – 2.7-7.4 %, amount of solid residue – 23.8-43.0 %. The highest weight of the bunch, stem, remaining skin and pulp, number of seeds, weight of 100 seeds, and the lowest juice yield were noted in table varieties; for wine grape varieties, this ratio was the opposite. Key words: GRAPE VARIETIES, LOCAL VARIETIES, ENOCARPOLOGICAL INDICATORS, BUNCH, BERRIES

МОНИТОРИНГ ВИНОГРАДНОГО ТРИПСА НА ВИНОГРАДНИКАХ

The publication presents the results of studies to determine the population size of grape thrips Drepanothrips reuteri Uzel and the extent of distribution of on the Don under different meteorological conditions. The studies were carried out in 2021 and 2022 in the conditions of two agrocenoses of Novocherkassk and Nizhnekundryuchensky Branches of the experimental field of the All-Russian Research Ya. I. Potapenko Institute for Viticulture and Winemaking. The subject of the study was the dynamics of the prevalence and harmfulness of grape thrips on the technical grape varieties Tsvetochnyi and Cabernet severnyi, in an uncovered culture. In the context of the widespread distribution of sugescent pests of grapes, the issue of protecting production plantings and improved basic plantings of the mother plant from them is acute, using the method of monitoring studies to signal the population size using flower traps. Therefore, the study of the development features of grape thrips, both in the conditions of the mother plant of basic plants and fruit-bearing vineyards and their influence on the economically valuable traits of grapes, is relevant. Grape thrips is a relatively new species for grape plants in the Rostov region, migrating from abandoned areas with weeds adjacent to the vineyards in search of food. To contain the prevalence of the grape thrips population below the economically significant threshold of harmfulness in agrocenoses, it is necessary to conduct timely phytosanitary monitoring. Under the conditions of two agrocenoses in 2021 and 2022, two generations of grape thrips developed during the growing season: the first at the end of May, the second at the end of June. The intensity of damage to plants of two grape varieties by grape thrips was not significant and did not exceed the economically significant threshold of harmfulness, so it was decided not to carry out chemical treatments. The presence of a stable number of pest individuals suggests that the population may be capable of wider distribution. Key words: GRAPEVINE, DREPANOTHRIPS REUTERI UZEL, POPULATION DYNAMICS, HARMFUL

Исследование качества молодых игристых вин из крымских автохтонных сортов винограда

Most domestic sparkling wines are made of traditional champagne grape varieties. However, indigenous Crimean cultivars could increase the output of high-quality original beverages if a proper technology was introduced. 
 This research featured young sparkling wines from Crimean grape varieties, i.e., Soldaya, Shabash, Kokur Beliy, Sary Pandas, Kefesiya, Dzhevat Kara, and Ekim Kara. Organic acids, sugars, glycerin, phenolic substances, and ethanol were determined by high performance liquid chromatography; the content of amine nitrogen was measured by formalin titration. The optical profile of the wine samples was subjected to the colorimetric method while their foamy properties were studied by bubbling the samples in a measuring cylinder. The sparkling properties depended on the rate of CO2 desorption; the volumetric method made it possible to define the CO2 content; the viscosity was measured using a viscometer. The sensory assessment followed State Standard 32051-2013, ISO 5492:2008, and ISO 11035:1994. 
 The samples of Kokur Beliy, Sary Pandas, and Soldaya received high tasting ratings (≥ 9.0 points), as did the red variety of Kefesiya (8.95 points). They demonstrated a clear typical aroma and a harmonious taste, as well as good foamy and sparkling properties. The maximal foam volume correlated with amine nitrogen (r = 0.762) while the sparkling properties correlated with the mass fraction of bound CO2 (r = 0.977). The red sparkling wine from the Kefesiya variety had a dark garnet color due to its high anthocyanins. It also contained quercetin and glycoside, which are known for their biological (P-vitamin) activity and powerful antioxidant properties. The early low-sugar (< 18 g/100 cm3) varieties of Dzhevat Kara and Ekim Kara had the same acidity but demonstrated a less diverse phenolic profile and were paler in color. 
 In this research, the indigenous Crimean grape cultivars of Kokur Beliy, Sary Pandas, Soldaya, and Kefesia showed good prospects for young sparkling wines. Other varieties needed special technologies to preserve their typical properties and the aroma/taste balance. The new technology makes it possible to obtain unique high-quality products right in the harvest year, thus increasing the range and volume of sparkling wine production.

Влияние винных и виноградных спиртов на качество напитков из виноградного сырья

Table grapes that have lost their marketable appearance are considered waste products. However, such grapes still possess some technological properties that make it possible to use them as a raw material for wine and grape spirits. This research featured the effect of the composition and technology of table grape alcohols on the volatile compounds and sensory profile 
 of grape beverages.
 The study involved six white and six red grape beverages. In the test samples, the fermentation process was stopped by adding wine spirits and grape spirits obtained by rectification of distillates from table grapes. In the control samples, the procedure involved rectified grain alcohol. The physicochemical parameters of drinks and spirits were defined by standard methods. The highly volatile compounds were determined by gas chromatography. The sensory assessment was conducted by panelists from the Winemaking Research Center of the North-Caucasian Federal Scientific Center of Horticulture, Viticulture, and Winemaking.
 In this study, the wine spirits and the grape spirits obtained from table grapes had no effect on the standard indicators, e.g., volatile acids and acetoin. However, they increased the mass concentrations of highly volatile compounds in the following manner: drinks with grain alcohol ˂ drinks with wine alcohol ˂ drinks with grape alcohol. The content of acetaldehyde increased by 10–14% while the contents of ethyl acetate, esters, and higher alcohols increased by 25–35, 4.5–8.5, and 15%, respectively. The furfural content increased by 0.4–1.4 mg/L in the samples with grape alcohol. The samples with white grape varieties acquired a more prominent floral-honey flavor whereas those with black grapes acquired hints of hazelnut and honey.
 As a result of the study, it was found that wine alcohol and grape alcohol moderately increased the content of volatile compounds in drinks and had a positive effect on their sensory perception, which proves the expediency of their use in beverage technology.

МЕТОДОЛОГИЯ УПРАВЛЕНИЯ АГРОБИОЛОГИЧЕСКОЙ, АДАПТИВНОЙ И ПРОДУКЦИОННОЙ УСТОЙЧИВОСТЬЮ НАСАЖДЕНИЙ ВИНОГРАДА В НЕСТАБИЛЬНЫХ ПОГОДНЫХ УСЛОВИЯХ И ТЕХНОГЕННОЙ ИНТЕНСИФИКАЦИИ ПРОИЗВОДСТВА

The article presents the results of agrobiological and physiological-biochemical studies to ensure the stability of grape plantations in unstable weather conditions and anthropogenic intensification of production. Studies in the agroecological conditions of the Krasnodar region on the southern chernozems revealed the most productive norm of loading of grape bushes with shoots for the technical varieties Kurchanskiy, Dmitriy, Vladimir, Granatovyi, Antaris and Alkor, on leached chernozems – for the table seedless variety Centennial Seedless. According to the results of electrophoretic separation of peroxidases in polyacrylamide gel and an increased content of ascorbic acid, increased frost resistance was established in the varieties Kristall, Vostorg, Krasnostop AZOS. The Vostorg and Zarif varieties proved to be more adaptive to the stresses of the summer period in terms of leaf hydration, bound water content, the ratio of chlorophylls and carotenoids, the amount of proline and water-soluble sugars, membrane permeability. The role of physiologically active substances in increasing the adaptive potential of grape plants has been established. The treatment of Merlot vines with methyl jasmonate and epibrassinolide during deep dormancy period, as well as proline, salicylic acid and epibrassinolide during induced dormancy period had a positive effect on the condition of the vine: there was a decrease in electrolyte yield. Sustainable grape cultivation is achieved with the systemic use of the «Bioconcentrate-Z» effluent by the non-root method. Against the background of nonroot treatments of Merlot and Cabernet Sauvignon varieties, a higher level of plant water supply, an increase in potassium content, green pigments, organic acids and grape yields were established. Physiologically active compounds and the preparation Turinbash have a positive effect in intensive own-root plantations of native and promising introduced grape varieties against the background of phylloxera infection in the southern part of Dagestan. The created yield forecasting method based on the NDVI of the grape plant and the actual data of multispectral images of the phenophases of flowering and growth allows to increase the accuracy of the calculated yield capacity to the actual one with a deviation from 0 to 0.8 kg per bush, the accuracy of the forecast increases by 2 times by the end of the growth phase and by the beginning of the maturation phase. Key words: GRAPES, METHODS, MANAGEMENT, SUSTAINABILITY, PRODUCTIVITY, QUALITY, FORECASTING

Study and evaluation of agrobiological indicators of grape varieties and hybrid forms in the conditions of Southern Dagestan

The article presents the results of many years of research on the study of the genetic fund of grapes concentrated in the ampelographic collection of the Dagestan selection experimental station for viticulture and vegetable growing. Selection and variety study of grapes play a major role in the accelerated promotion to the market and the introduction into industrial production of promising varieties for various uses, which is very important in our time. A comprehensive study of 104 varieties and hybrid forms of grapes of intraspecific and interspecific origin was carried out. The purpose of the study is an agrobiological assessment of promising varieties and hybrid forms of grapes of interspecific and intraspecific origin for various uses. Methods. The research was carried out at the ampelographic collection of the Dagestan selection experimental station for viticulture and vegetable growing, located in Derbent. The object of research was 104 varieties and hybrid forms of grapes of domestic and foreign selection. Of these, 30 table varieties, 70 technical varieties and 4 universal varieties. The zoned varieties Karaburnu (table) and Muscat white (technical) were used as a standard. The research was carried out according to the following methods: “Study of grape varieties” and “Agrotechnical research on the creation of intensive grape plantings on an industrial basis”. Results. The article presents the results of assessing the indicators of fruiting and fruitfulness of varieties and hybrid forms of grapes of interspecific and intraspecific origin. According to the indicators of fruiting (K1) and fruitfulness (K2) among table varieties and hybrid forms, the following are distinguished: SV-23-657, SV-12-304, Dekabrsky, Ialoveni sustainable, V-95-1, XI-36-6/100 of which K1 ranged from 0.94 to 1.33 and K2 from 1.17 to 1.51. Among the technical varieties and hybrid forms based on these indicators, the following were distinguished: Bianca, Viorica, Gift of Magaracha, Yubileiny Magaracha, Pervenets Magaracha, in which K1 ranged from 1.17-1.50 and K2 from 1.34-1.60. For most varieties and hybrid forms, the fruiting coefficient (K1) varies from 0.67 to 1.30, and the fruiting coefficient (K2) ranges from 1.03 to 1.42. In universal varieties, the fruiting coefficient (K1) ranges from 0.70-1.02 and the fruiting coefficient ranges from 0.96-1.31. According to the yield of table varieties, interspecific hybrids are distinguished: Dekabrsky, SV-12-304, SV-20-365, SV-23-657, XI-36-6/100 in which the yield per bush varies from 11.2 to 19.5 kg/bush. Among the technical varieties in terms of yield per bush, the following stand out: TSKHA-3-2, Pierrel, Bianka, SV-20-473, Strogoziya, Podarok Magaracha, SV-12-375, for which the yield per bush was 12.4-22.2 kg. Conclusion. The introduction of selected varieties and hybrids into production will ensure an increase in crop yields and will contribute to import substitution and ensure the country's food security. Individual varieties will be used as sources of valuable traits in breeding work.

Advancements in deep learning for accurate classification of grape leaves and diagnosis of grape diseases

AbstractPlant diseases cause significant agricultural losses, demanding accurate detection methods. Traditional approaches relying on expert knowledge may be biased, but advancements in computing, particularly deep learning, offer non-experts effective tools. This study focuses on fine-tuning cutting-edge pre-trained CNN and vision transformer models to classify grape leaves and diagnose grape leaf diseases through digital images. Our research examined a PlantVillage dataset, which comprises 4062 leaf images distributed across four categories. Additionally, we utilized the Grapevine dataset, consisting of 500 leaf images. This dataset is organized into five distinct groups, with each group containing 100 images corresponding to one of the five grape types. The PlantVillage dataset focuses on four classes related to grape diseases, namely Black Rot, Leaf Blight, Healthy, and Esca leaves. On the other hand, the Grapevine dataset includes five classes for leaf recognition, specifically Ak, Alaidris, Buzgulu, Dimnit, and Nazli. In experiments with 14 CNN and 17 vision transformer models, deep learning demonstrated high accuracy in distinguishing grape diseases and recognizing leaves. Notably, four models achieved 100% accuracy on PlantVillage and Grapevine datasets, with Swinv2-Base standing out. This approach holds promise for enhancing crop productivity through early disease detection and providing insights into grape variety characterization in agriculture.

ФИЗИОЛОГО-БИОХИМИЧЕСКИЕ ИЗМЕНЕНИЯ В ПОБЕГАХ ВИНОГРАДА В ПЕРИОД ЗИМНЕГО ПОКОЯ

The adaptive of the stability of cultivated grape varieties are changing due to the instability of the climate in the Anapa-Taman zone of the Krasnodar region. The purpose of this work is to conduct a comparative analysis of the physiological and biochemical changes occurring in the shoots of grape varieties with different frost resistance during the winter dormancy. The objects of research are grape varieties of various genetic origin: Kristall (control), Dostoynyi, Krasnostop AZOS, Vostorg, Aligote, Zarif. On the basis of generally accepted physiological and biochemical methods, the features of physiological and biochemical changes in grape varieties of different frost resistance in the autumn-winter period 2021-2023 were studied. It was revealed that the ripened shoots of grapes were characterized by both changes in the anatomical structure of tissues (formation of periderm, hardening and lignification of cell walls) and physiological and biochemical changes in the cells of storage tissues – a high content of starch and sugars, as well as a decrease in hydration. It was revealed that the hydration of shoots decreased to a greater extent in frost-resistant varieties Kristall, Dostoynyi, Krasnostop AZOS, Vostorg – by 5.07-5.29 %, in non-frost-resistant varieties Aligote, Zarif – by 1.25-2.04 %. It was found that a more complete conversion of starch into sugar occurred in frost-resistant varieties of Kristall, Dostoynyi, Krasnostop AZOS, Vostorg. Thus, the starch content decreased in varieties: Crystal – by 2.51 times, Dostoynyi – by 2.15 times, Krasnostop AZOS – by 2.21 times, Vostorg – by 2.06 times. In the non-frost-resistant Aligote variety, starch reduction was 1.43 times, and in Zarif – 1.40 times. It was shown that a higher content of organic acids, important antioxidants in protection against oxidative stress, was noted in more frost-resistant varieties – Kristall, Dostoynyi, Krasnostop AZOS, Vostorg. – 10.8-17.2 mg/g of fresh weight, compared with non-frost-resistant Aligote, Zarif – 5.28-7.50 mg/g of fresh weight. The relationship between the content of water, starch, sugars, organic acids and frost resistance of grape varieties has been revealed. The less hydration of the shoots, the greater the degree of sugar formation, the higher the content of organic acids in the tissues of the shoot, the more frost-resistant the variety. Key words: GRAPES, DORMANCY, STARCH, SUGARS, ORGANIC ACIDS, FROST RESISTANCE