Grape Rootstocks Research Articles

Behavioral Response of Grape Root Borer (Lepidoptera: Sesiidae) Neonates to Grape Root Volatiles

Grape root borer, Vitacea polistiformis (Harris), is an oligophagous and potentially destructive pest of grape in commercial vineyards throughout much of the eastern United States. Larvae feed on vine roots, although little is known about their below-ground interactions with host plants. The behavioral response of groups of grape root borer neonates to stimuli from host and nonhost roots was evaluated in single and paired stimuli bioassays in which stimuli were presented in opposing wells attached to the bottom of petri dish arenas. Stimulus sources included root pieces and root headspace volatiles from 3309 and 420-A grape rootstocks (host) and apple (nonhost) and ethanol-based extracts of 3309 and 420-A roots. In single stimulus assays, significantly more larvae were recovered from wells containing grape roots, apple roots, grape extracts, and grape root volatiles than from control wells, but there was no significant response to volatiles collected from the headspace of apple roots. In paired stimuli assays, significantly more larvae were recovered from wells containing grape than apple roots. There was no difference in larval distribution between wells when 420-A and 3309 roots were presented simultaneously, although a significantly greater response to 3309 than 420-A root extract was recorded. When soil was added to the assays, significantly more larvae were recovered from wells containing grape roots than from those containing only soil, but this response was not detected in assays using buried apple roots. These results are discussed in relation to the plant-insect interactions between grape root borer larvae and their Vitaceae hosts.

Micropropagation using a bioreactor system and subsequent acclimatization of grape rootstock ‘5BB’

To improve bioreactor culture systems for the micropropagation of grape rootstock ‘5BB,’ the effects of inoculation density, air volume, and light intensity on plantlet growth were investigated. Maximum plantlet growth and proliferation were observed when 65 nodes were inoculated in a 5l balloon-type airlift bioreactor system, aerated with 150mlmin−1 air, and maintained under 50μmolm−2s−1 light intensity. During bioreactor culture, plantlet growth increased with increasing number of culture days, but no changes were observed between days 40 and 50; thus, 40d was considered a suitable culture period. Electric conductivity and sucrose in the culture medium were negatively correlated with plantlet biomass. Several substrates were tested during acclimatization of the plantlets derived from the bioreactor, and vermiculite was observed to be the most advantageous substrate for transplantation. In this substrate, almost all of the plantlets survived and leaf stomata recovered their close function on the 16th day after transplantation.

The effect of media composition on multiplication of grape rootstocks in vitro

The current demand for in vitro cultures of grape rootstocks, not only for mass production of plants, but also for genetic engineering is evident. The study on micropropagation of grape rootstock genotypes namely Kober 5BB, Kober 125AA and Teleki 5C was performed. The aim of the study was to develop an optimized protocol to obtain large quantity of plant material. Protocol is based on regeneration via organogenesis, considering that grape embryogenic calluses are laborious to establish and the genotype of the regenerated plants can be altered. Using of Driver and Kuniyuki Walnut media for the establishing of proliferating cultures gave better results than Murashige Skoog media in case of all used rootstocks. Subsequent cultivation on modified Murashige Skoog media with 1-naphtalene acetic acid and increased concentration of cytokynin was characterized by multiplication of cultures and formation of clusters with high multiplication capability. The clusters obtained from rootstock genotypes were suitable for mass propagation as well as for genetic transformation due to their high ability of regeneration.

The impact of various grape stock cultivars on the As, Cu, Co and Zn content of the grape berry (must, seed)

Scientific research from the last decades showed that the inappropriate industrial and agricultural production caused an abnormal increase of the potentially toxic elements in the soil. Unfortunately the acidification of the soil is an increasing problem in Hungary. According to Várallyay et al. (2008) 13% of the Hungarian soils are highly acid. Accumulation of toxic elements differs in the genetically diverse plant species. The root of the plant constitutes a filter so that the rootstock is also kind of a filter system, which may prevent that the scion part (such as berry) accumulate high levels of various potentially toxic elements from the soil. The aim of research was to determine how different grape rootstocks influence the As, Co, Cu and Zn content of the musts and seeds. Thus, specifying which of the grape rootstocks takes up the lowest level of these 4 elements (As, Co, Cu and Zn), and accumulates in berries, so could reduce the potentially toxic element load of the grape berries. The grape rootstock collection of the University of Debrecen was set up in 2003 in 3x1 m spacing on immune sandy soil. Grafting of ‘Cserszegi fűszeres’ was started in 2010. We could evaluate yields harvested from 12 rootstock varieties of the experiment in October 2011. We obtained valuable differences in the arsenic, copper, cobalt and zinc concentrations of musts and seeds of ‘Cserszegi fűszeres’ grafted into different rootstocks. The results obtained from the 2011 harvest support the statement that the choice of rootstock might be an important factor to increase food safety. The differences in concentration of the four elements observed in case of the rootstock may have been caused on one hand by the rootstock effect, and on the other hand, the vintage effect has a very significant impact on the vines element uptake. Several years of experimental results will be needed to answer these questions.

A PRELIMINARY STUDY ON UTILIZATION OF DIFFERENT HENNA EXTRACTS COMPARED WITH IBA ON ROOTAPILITY OF DOGRIDGE GRAPE ROOTSTOCK CUTTINGS

The present work was carried out during the two successive seasons of 2010 and 2011 in the shade house of the Viticulture Department, Horticulture Research Institute, in Giza, Agric. Res. Center, Ministry of Agriculture. The used cuttings were taken from mother Dogridge vines grown in the private farm at 58 Km Cairo - Alex. desert road. Hard-wood cuttings were taken in Jan. from moderately vigorous 1-year-old canes, each cutting comprised three nodes (eyes) and was about 30-35 cm long. The cuttings were stored with their basis upwards in the shade house until planting on 15 Feb. in both seasons Henna extracts obtained from the crushed dried leaves of Lawsonia inermis L. 200 gm Henna was immerged in one liter for 48 hours in one of the following solvents:- tap water, boiled water, ethyl alcohol, hexane, acetone and petroleum ether. The used concentration from henna crude extracts by different solvents was 3gm/ l of distilled water (i.e.3000 ppm). Cuttings were soaked in each of the considered extracts for 24 hours before planting. In addition, the recommended application of the growth regulator IBA at (1000 ppm) by dipping for 10 seconds was used as a control. The best results were obtained by henna extracted by acetone and tap water solvent without significant differences among them. While, untreated cuttings gave the lowest results followed by cuttings treated by ethyl alcohol extract and than IBA treatment. The other henna extractions were ranged between them. Generally, it is possible to improve rootapility of Dogridge grape rootstock cuttings by soaking cutting basis in henna extracted by acetone or tap water solvents which gave nearly similar effect, but use of tap water solvent, is more economic and easier for application.

Genetic and virulence diversity, and mating type distribution of Togninia minima causing grapevine trunk diseases in Spain

Fifty eight single-spore Togninia minima (anamorph Phaeoacremonium aleophilum) isolates were recovered from grape rootstock wood of plants that showed symptoms of Petri disease and esca from 2001 to 2008 in Spain. These isolates were studied by means of mating type distribution, UP-PCR analysis, and virulence assays. Analysis of clone-corrected data sets showed equal frequencies of both mating types in the entire Spanish population, in the Ciudad Real region, at inter-vineyard and intra-vine spatial scales; while unequal mating type distribution was detected in Valencia and Zaragoza regions, at intra-vineyard and intra-vine spatial scales. This is the first study on distribution of T. minima mating types on spatial scales varying from vineyards to regions. A total of 49 polymorphic UP-PCR markers were obtained using seven UP-PCR primers. Four optimal clusters were inferred with Bayesian structure and multivariate analyses from the UP-PCR data. The high number of unique genotypes observed within the Spanish population, combined with a near-equal distribution of mating types, suggested that sexual reproduction probably does occur. However, based on allele distribution and frequency, each of the three subpopulations appeared to be evolving independently. Gene and genotype diversities across the subpopulations were similar and ranged from 0.24 to 0.27 and from 0.27 to 0.37, respectively. The detection of genetically identical isolates within and among subpopulations indicates that an asexual reproductive component should not be excluded. Contrast analysis among groups defined by UP-PCR analyses showed no significant differences in the virulence of T. minima isolates.

In vitro propagation and preservation of important grape cultivars (Vitis vinifera L.) and rootstocks

In vitro propagation and preservation of important grape cultivars (Vitis vinifera L.) and rootstocks

AŞI UYUŞMA DÜZEYİ BELİRLENMESİNDE KULLANILAN FORMÜLLERİN FARKLI ÜZÜM ÇEŞİT-ANAÇ KOMBİNASYONLARINDA KARŞILAŞTIRILMASI

Vitis vinifera L. cultivars have to be grafted on American grape rootstocks due to phylloxera and nematode damages, which makes it obligatory to determine their graft compatibility in a vineyard. For this purpose, any of four formulae developed by Perraudine, Branas, Spiegel-Roy & Lavee and Onaran was inconclusive to reliably determine the compatibility of a scion/stock combination. Therefore, it is needed to compare these results with values of pruning weight, yield and quality analyses obtained from a long-term grape growing. In addition, they have to be supported by histological and biochemical analyses.

Biochemical changes in grape rootstocks resulted from humic acid treatments in relation to nematode infection

ObjectiveTo investigate the effect of humic acid on nematode infected, resistant and susceptible grapes in relation to lipid peroxidation and antioxidant mechanisms on selected biochemical parameters known as proactive substances. MethodsThe grape rootstocks, superior, superior/freedom and freedom were reacted differently to Meloidogyne incognita and Rotylenchulus reniformis according to rootstock progenitor. Two weeks after inoculation, two commercial products of humic acid were applied at the rate of (2, 4 mL or grams/plant) as soil drench. After 4 months, nematode soil populations were extracted and counted. A subsample of roots from each plant was stained and gall numbers, embedded stages per root were calculated, final population, nematode build up (Pf/Pi), average of eggs/eggmass were estimated. Subsamples of fresh root of each treatment were chemically analyzed. ResultsFreedom reduced significantly the nematode criteria and build up. Humic acid granules appeared to be more suppressive to nematode build up on superior and the higher dose on superior/freedom than liquid treatments. On freedom, all treatments reduced significantly the nematode build up regardless to the material nature. The higher dose was more effective than the lower one. As a result of humic acid applications, the malondialdehyde (MDA) and H2O2 contents were significantly reduced after humic acid treatments while the antioxidant compounds glutathione (GSH), ascorbic acid (ASA) and total phenol contents were significantly increased when compared with check. Antioxidant defense enzymes ascorbate peroxidase (APX), superoxide dismutase (SOD), catalase (CAT) and polyphenol oxidase (PPO) showed significant increase in their specific activities in treated plants compared with nematode treated check. ConclusionsHumic acid treatments improve the yield of grape by increasing the contents of antioxidant compounds and the specific activities of antioxidant enzymes.

Anatomy and osmotic potential of the Vitis rootstock shoot tips recalcitrant to cryopreservation

This study was carried out on Kober 5BB (Vitis Berlandieri × V. riparia) grape rootstock shoot tips during the preparatory steps preceding the direct immersion in liquid nitrogen, in order to overcome until now unsuccessful cryopreservation with this species. The exposure of shoot tips to 0.3-0.4 M sucrose leads to a high cell solute concentration. The treatment with plant vitrification solution (PVS2) alone, i.e., not followed by storage in liquid nitrogen, markedly affected shoot tip survival. After a 30 min exposure, regrowth percentage of shoot tips decreased from 94 % (control) to 57 %, and dropped to 15 % when the treatment was prolonged up to 60 min. After a 90 min exposure, no regrowth occurred. In addition, plantlets regenerated from shoot tips which underwent 60 min or more exposure to PVS2 showed signs of malformation. Microscope observations of shoot tips treated with 0.3 or 0.4 M sucrose and 30 min PVS2 showed the presence of cells starting to plasmolyze, localized in the area surrounding the apical meristem. A limited presence of starch grains in meristem and bract cells was also noted. However, the most conspicuous consequence of prolonged PVS2 treatment was convex plasmolysis. The phenomenon was dependent on the time of PVS2 exposure. Indeed, after a 30 min treatment, plasmolysis was minimal or absent, but it increased with longer exposure to PVS2 at 4 °C.

Root length, root mass, and distribution of dry matter in different parts of Thompson Seedless grapevine grafted on different rootstocks in heavy soil of Maharashtra

The root distribution pattern of 3 grape rootstocks, Dog Ridge, Salt Creek, and St. George, grafted with Thompson Seedless grapevine was studied at the National Research Centre for Grapes in Pune, India. The soil is heavy black cotton soil (vertisol) with a pH of >8.0. Roots of different thicknesses (10 mm) were examined at horizontal distances of 0-30 cm, 31-60 cm, 61-90 cm, 91-120 cm, and 121-150 cm away from the trunk and also to depths of 0-30 cm, 31-60 cm, and 61-90 cm from the surface. Among the different categories of roots, Dog Ridge put forth the maximum root length in the

Analysis of grape rootstocks by SSR markers

<p style="text-align: justify;"><strong>Aims</strong>: The aim of this work was to determine the SSR profile of 96 <em>Vitis</em> accessions (mainly rootstocks) at 19 loci to find genetic relatedness between them.</p><p style="text-align: justify;"><strong>Methods and results</strong>: The molecular markers used in the last 30 years are not or less affected by the environment, making them a valuable tool for the determination of genetic distances. Based on our former experiences, an SSR analysis was carried out on 96 grape accretions, mainly rootstocks. DNA was extracted from young leaves and analyses were carried out at 19 microsatellite loci. The loci were selected to cover all of the linkage groups to get a more accurate genotyping of each grape rootstock. Results of the SSR analysis and the resulting dendrogram showing genetic relatedness are presented.</p><p style="text-align: justify;"><strong>Conclusions</strong>: Based on the results, it can be established, that most of the selected loci showed appropriate polymorphism for the assessment of genetic relatedness. The rootstocks derived from Teleki’s seedlings showed high similarity. A low genetic distance was also detected between the accessions originated from the cross between Börner and Georgikon 28. Finally, the <em>Vitis sylvestris</em> accessions showed close relatedness with the <em>Vitis vinifera</em> L. varieties.</p><p style="text-align: justify;"><strong>Significance and impact of the study</strong>: Our results indicate a high level of polymorphism between grape rootstock accessions. Strong differences were detected between the so-called Teleki 5C (T5C) and Teleki-Kober 5BB (T5KBB) “clones”, suggesting that they are not real clones but different genotypes with highly similar morphological features.</p>

Anatomy and osmotic potential of the Vitis rootstock shoot tips recalcitrant to cryopreservation

This study was carried out on Kober 5BB (Vitis Berlandieri × V. riparia) grape rootstock shoot tips during the preparatory steps preceding the direct immersion in liquid nitrogen, in order to overcome until now unsuccessful cryopreservation with this species. The exposure of shoot tips to 0.3-0.4 M sucrose leads to a high cell solute concentration. The treatment with plant vitrification solution (PVS2) alone, i.e., not followed by storage in liquid nitrogen, markedly affected shoot tip survival. After a 30 min exposure, regrowth percentage of shoot tips decreased from 94 % (control) to 57 %, and dropped to 15 % when the treatment was prolonged up to 60 min. After a 90 min exposure, no regrowth occurred. In addition, plantlets regenerated from shoot tips which underwent 60 min or more exposure to PVS2 showed signs of malformation. Microscope observations of shoot tips treated with 0.3 or 0.4 M sucrose and 30 min PVS2 showed the presence of cells starting to plasmolyze, localized in the area surrounding the apical meristem. A limited presence of starch grains in meristem and bract cells was also noted. However, the most conspicuous consequence of prolonged PVS2 treatment was convex plasmolysis. The phenomenon was dependent on the time of PVS2 exposure. Indeed, after a 30 min treatment, plasmolysis was minimal or absent, but it increased with longer exposure to PVS2 at 4 °C.

Bioproduction of resveratrol and viniferins by an elicited grapevine cell culture in a 2 L stirred bioreactor

A cell suspension culture was developed from calli of grape rootstock 41B in order to study the bioproduction of resveratrol. While 41B grape cultures produced no resveratrol, methyljasmonate (MeJA) elicitor treatment activated its production in a dose dependent manner. The concentration of 0.2mM MeJA was optimal for efficient production and high accumulation of resveratrol (150mg/L) in flask experiments. Microscopic analysis of cells monitored for viability showed that MeJA elicitor triggered expression of resveratrol fluorescence within the cells. These results led to scale-up of the culture in a 2L stirred bioreactor where a resveratrol production of 209mg/L being secreted into the liquid medium, corresponding to 90% of the total production. Liquid/liquid extraction of the culture medium and a solid/liquid extraction of the cells showed that other stilbenes were also produced. For the first time, trans-ɛ-viniferin, trans-δ-viniferin, and a trans-3-methylviniferin as well as trans-piceatannol were identified in a 2L bioreactor cell cultures of grapevine. Furthermore, a one step FPLC method was developed for the purification of resveratrol and ɛ-viniferin.

Fungos micorrízicos arbusculares (FMA) em porta-enxertos micropropagados de videira

O uso de fungos micorrízicos na produção de mudas de videira é importante alternativa para acelerar o desenvolvimento inicial das plantas. Este trabalho teve por objetivo avaliar o comportamento de dois FMA sobre o crescimento vegetativo de três porta-enxertos (PE) de videira. Foram utilizados os FMA Glomus etunicatum e Scutellospora heterogama e os PE SO4, Paulsen 1103 e 043-43. Os PE, oriundos de micropropagação, foram transferidos e aclimatizados em copos plásticos de 300 mL e após 58 dias transplantados para sacos plásticos de 2 L de capacidade, contendo como substrato uma mistura de casca de arroz carbonizada e Plantmax® na proporção de 1:1 v/v. Utilizaram-se, como inóculo, 20 g de solo rizosférico mais fragmentos de raízes contendo as estruturas dos FMA, adicionados ao substrato na fase de aclimatização. Aos 135 dias após inoculação, foram avaliadas as massas de matéria fresca e seca das raízes e parte aérea, comprimento de raízes e parte aérea, diâmetro de colo e teores de nutrientes da parte aérea. Os FMA Glomus etunicatum e Scutellospora heterogama proporcionaram melhor nutrição e maior crescimento vegetativo dos porta-enxertos de videira, se comparadas as plantas testemunhas. Os benefícios oriundos da simbiose dependeram da combinação específica entre o isolado fúngico e a variedade de porta-enxerto utilizada, sendo o 043-43 beneficiado preponderantemente pelo FMA Glomus etunicatun e o SO4 e Paulsen 1103 por Glomus etunicatum e Scutellospora heterogama.

Rooting behaviour, polyphenol oxidase activity, and biochemical changes in grape rootstocks at different growth stages

Four grape rootstocks belonging to different Vitis species were planted in September. Rooting behaviour, polyphenol oxidase (PPO; EC 1.14.18.1) activity and biochemical parameters were studied at different growth stages after planting the cuttings in polythene bags. Significant differences were recorded for rooting success among the different rootstocks, with the maximum sprouting percentage determined in the Freedom rootstock. The highest variation in PPO activity was also recorded in Freedom. High PPO activity was recorded in rootstock 140 Ru, while the highest root length was recorded in Dog Ridge. In the rootstock 110 R, the PPO activity was the lowest during the initial stage, though it increased up to 60 days after planting (DAP) and was then reduced up to 90 DAP. Increases in the number of rooting primordials were recorded in all the different rootstocks at different stages of growth. A higher number of rooting primordials was recorded in Freedom, and it was followed by 110 R. The present study suggests the differences in the rooting behaviour of the different rootstocks are based on PPO activity at regular time intervals up to 90 DAP.

Response of grape rootstocks to salinity: changes in root growth, polyamines and abscisic acid

Effects of salinity (0, 50, 100 and 250 mM NaCl) on growth, root:shoot dry mass ratio, osmotic potential (ψx), electrolyte leakage and contents of Na+ and K+, polyamines and abscisic acid (ABA) were studied in the grape rootstocks Dogridge, 1613, St. George and Salt Creek. In control rootstocks, the root length was highest in Dogridge and contents of K+ and ABA in Salt Creek. Salinity treatments increased root Na+ and decreased K+ content and St. George exhibited highest Na+ content and Na+:K+ ratio. The root:shoot dry mass ratio in all rootstocks increased upto 100 mM NaCl. With increasing NaCl concentration, putrescine, spermine and spermidine contents showed consistent increase and putrescine increase was highest in St. George and spermidine and spermine in the Dogridge and Salt Creek. Under salinity, the ABA content increased in all the rootstocks but more in Salt Creek and Dogridge than in St. George.

In vitro propagation of a grape rootstock, deGrasset ( Vitis champinii Planch.): Effects of medium compositions and plant growth regulators

The aim of the present study was to develop a protocol for in vitro propagation of a grape rootstock, deGrasset, characterized by high tolerance to drought and salinity. Four medium compositions, MS, MS with 1/2 nitrates (MS-1), B5 and WPM, were tested for shoot growth from nodal explants and MS-1 medium produced significantly higher rate of shoot proliferation. MS-1 medium supplemented with 2.0 mg/L BAP was found to be optimum for culture establishment. The first subculturing on the same medium resulted in the production of 4–6, mostly short, hyperhydrated shoots per explant. For subsequent subcultures, a reduced concentration of BAP (1.0 mg/L) was used to prevent hyperhydricity, and that resulted in distinct individual shoot elongation. Three plant growth regulators, BAP, ZEA and TDZ, were tested for further shoot proliferation and BAP at 1.0 mg/L added to MS-1 medium displayed the highest proliferation rate (4.75 new explants per explant inoculated, in 6 weeks). For in vitro rooting of shoots, IAA at 0.2 mg/L was found to be optimum to produce highest response (84%) and an average number of 2.03 roots per shoot whereas use of IBA or NAA resulted in rooting with high frequency of callus formation. The acclimatized plantlets were established in soil under net house conditions with 87% success.

Greenhouse Screening of Grape Rootstock Populations to Determine Inheritance of Resistance to Phylloxera

Phylloxera has been an important pest in California since its discovery in 1880 within Sonoma and Napa counties. Phylloxera-resistant rootstocks and germplasm were selected from American grape species native to the eastern United States. Breeding programs to develop improved phylloxera-resistant rootstocks were started in the late nineteenth century. Resistance to phylloxera has been reported to be controlled by several genes. To focus on one aspect of resistance to phylloxera, a greenhouse screening method was used to observe absence/presence of root nodosities produced by phylloxera. Only one source of phylloxera biotype A from Fresno County was used to reduce the complication of various biotypes. Grape rootstocks with known field reaction to phylloxera were evaluated to test the reliability of the greenhouse test. A design II mating factorial cross was made between male and female rootstocks with a range of resistance to susceptibility. The reaction of their progeny to phylloxera was observed in the greenhouse. All populations segregated for resistance/susceptibility with a few exceptions. Dog Ridge crossed with two susceptible genotypes gave all susceptible offspring. Kober 5BB crossed with susceptible or resistant genotypes gave all resistant offspring. The segregation of resistance to nodosity development could be explained by two complementary dominant genes in most families.

Cloning and characterization of XiR1, a locus responsible for dagger nematode resistance in grape

The dagger nematode, Xiphinema index, feeds aggressively on grape roots and in the process, vectors grapevine fanleaf virus (GFLV) leading to the severe viral disease known as fanleaf degeneration. Resistance to X. index and GFLV has been the key objective of grape rootstock breeding programs. A previous study found that resistance to X. index derived from Vitis arizonica was largely controlled by a major quantitative trait locus, XiR1 (X. index Resistance 1), located on chromosome 19. The study presented here develops high-resolution genetic and physical maps in an effort to identify the XiR1 gene(s). The mapping was carried out with 1,375 genotypes in three populations derived from D8909-15, a resistant selection from a cross of V. rupestris A. de Serres (susceptible) × V. arizonica b42-26 (resistant). Resistance to X. index was evaluated on 99 informative recombinants that were identified by screening the three populations with two markers flanking the XiR1 locus. The high-resolution genetic map of XiR1 was primarily constructed with seven DNA markers developed in this study. Physical mapping of XiR1 was accomplished by screening three bacterial artificial chromosome (BAC) libraries constructed from D8909-15, V. vinifera Cabernet Sauvignon and V. arizonica b42-26. A total of 32 BAC clones were identified and the XiR1 locus was delineated within a 115 kb region. Sequence analysis of three BAC clones identified putative nucleotide binding/leucine-rich repeat (NB-LRR) genes. This is the first report of a closely linked major gene locus responsible for ectoparasitic nematode resistance. The markers developed from this study are being used to expedite the breeding of resistant grape rootstocks.