Granulocytosis Research Articles

Clinical features of bacterial liver abscess in diabetic patients

This retrospective analysis showed that the most frequent pathogen causing bacterial liver abscess was Klebsiella pneumoniae in 138 patients.Compared with the patients without diabetes mellitus,it was found that:( 1 ) the percentage of diabetic patients having typical abdominal pain was lower ( P ＜ 0.05 ) ; ( 2 ) neutrophilic granulocytosis was more marked,but albumin and hemoglobin levels were lower in diabetic patients( P＜0.05 ) ; ( 3 )more diabetic patients were complicated with urinary tract infection and suffered from septicemia( P＜0.05 ) ; (4) the clinical course of treatment in diabetic patients was much more prolonged( P＜0.05 ). Key words: Bacterial liver abscess; Diabetes mellitus; Clinical feature

Granulocytosis and Its Correlation to Lymphoproliferative

自己免疫疾患モデル動物の一系であるMRL/lprマウスは, 抗DNA抗体や抗Sm抗体など多彩な自己抗体の産生を伴う全身性エリテマトーデス (SLE) を自然発症する. このマウスにみられる大きな特徴は, このような自己免疫疾患の発症に随伴して, 生後2-3カ月頃より全身に著明なリンパ節腫脹が現れることである. このリンパ増殖症の発現は常染色体劣性のlpr遺伝子の働きによるもので, 増殖する細胞は, CD4 (L3T4) 分子とCD8 (Ly-2) 分子のいずれをも持たないユニークなTリンパ球 (lpr細胞) であることが明らかとなっている. 今回, われわれはこのMRL/lprマウスに出現する骨髄リンパ系の異常を調べる目的で, このマウス系の末梢血・骨髄, および各リンパ組織の加齢変化を検討した. その結果, MRL/lprマウスの末梢血中では, 好中球および単球が2カ月齢より増加しはじめ, 加齢に伴いいちじるしく増加することを発見した. このうち好中球の増加は骨髄中にもいちじるしく, 末梢好中球の増加は, 骨髄における増殖亢進を反映するものと考えられた. また, 6カ月齢では骨髄の形質細胞が増加した. 一方, 各臓器におけるlpr細胞の出現時期を調べたところ, この細胞は2カ月齢ごろにまずリンパ節や脾臓に出現し, その2カ月後に, 胸腺や血中・肺などに現れることがわかった. しかし, 骨髄にはいずれの時期にもこの細胞は出現しなかった. このように骨髄の好中球増殖と, リンパ増殖症とは異なる組織に発症する病態ではあるが, 時期的に同時に起こる現象であることから, 両病態の発症機構に何らかの液性因子を介した関連性のあることがうかがわれた. 1つの可能性はMRL/lprマウスの末梢血や骨髄における好中球増多に, 増殖するリンパ球由来の顆粒球増殖因子が関与していることであり, この点を検討するために, 穎粒球増殖因子の1つであるG-CSFを, BALB/cとMRL/nマウスに投与してみた. その結果, いずれの系統でも好中球の増殖がみられ, その程度は6カ月齢のMRL/lprマウスに相当するものであった. しかし, これらのマウスにはMRL/lprマウスにみられるような単球と形質細胞の増加, ならびに高免疫グロブリン血症は出現しなかった. これらの結果をもとに, MRL/lprマウスの顆粒球増多症に関連する可能性のある, 種々のサイトカインについて考按を加えた.

Effect of protein depletion and short-term parenteral refeeding on the host response to interleukin 1 administration.

Previous studies have demonstrated that protein malnutrition adversely affects the synthesis and release of interleukin 1 by monocytic cells. The purpose of this study was to investigate the protein and trace metal metabolism of severely protein-malnourished guinea pigs given exogenous interleukin 1 in the postabsorptive state or while concomitantly receiving total parenteral nutrition (TPN). Protein-depleted guinea pigs in the postabsorptive state failed to exhibit fever, granulocytosis, accelerated amino acid oxidation, or an acute-phase protein response after administration of interleukin 1 or endotoxin. However, a reduction in the serum concentration of zinc (P less than 0.05) and iron (P less than 0.05) was observed in the guinea pigs given interleukin 1, but not in those given endotoxin. The short-term (1 day) administration of TPN restored positive leucine balance (P less than 0.001) and reduced leucine release from protein breakdown (P less than 0.001). No other differences in the protein metabolism or trace metal response to interleukin 1 were observed as a result of short-term TPN feeding. Body temperatures of these malnourished guinea pigs given interleukin 1 and TPN became febrile or hypothermic depending on initial body temperatures. It is concluded that an attenuated capacity to synthesize interleukin 1 and a failure to mount an appropriate acute-phase protein response to exogenously administered interleukin 1 exist in this protein-malnourished animal model. Furthermore, this diminished protein response appears to be independent of short-term substrate provision because TPN for 1 day was unable to restore the full effects of the acute-phase response as mediated by interleukin 1.

Establishment of a cell line of gallbladder carcinoma (GBK-1) producing human colony stimulating factor.

A cell line designated GBK-1 was established from a patient with anaplastic carcinoma of the gallbladder, marked neutrophilia and fever, and has been propagated for the past 18 months. The cells grew as a monolayer sheet with a doubling time of 43 hr. The GBK-1 cells were of a pleomorphic polygonal epitheloid shape and they were transplantable into nude mice. Mice bearing the tumor developed marked granulocytosis. In the culture supernatant of GBK-1 cells, high colony stimulating factor (CSF) activity was evident with both human bone marrow cells and C57BL mouse bone marrow cells, and granulocytic colonies, macrophage colonies or granulocyte-macrophage mixed colonies were produced. The CSF activity was distributed in the molecular weight range of 25,000 to 60,000 with two distinct peaks at molecular weights of approximately 50,000 and 30,000. CSF activity was inactivated by heat treatment at 70 degrees for 30 min. GBK-1 is a new human cell line that produces heat-labile human GM-CSF.

Recombinant human granulocyte colony-stimulating factor induces granulocytosis in vivo.

Recombinant human granulocyte colony-stimulating factor (G-CSF) was prepared and its granulopoietic effects on mice were examined. When mice were injected intraperitoneally with the G-CSF daily for 14 days, marked granulocytosis occurred in the mice. The number of progenitor cells (CFU-C) was remarkably increased in the spleen. The results suggest that G-CSF plays a central role in granulocyte production in vivo.

A case of granulocytosis ocurring during the course of penicillin therapy

A case of granulocytosis ocurring during the course of penicillin therapy