Event Abstract Back to Event Different role of nitric oxide on adhesiveness and apoptosis of inflammatory granulocytes after burn injury Biljana Draskovic-Pavlovic1, 2*, Dragana Vucevic1, 2, Ivana Majstorovic1, Biljana Bozic3, Gordana Zunic1, 2 and Miodrag Colic2 1 Institute for Medical Research Military Medical Academy, Serbia 2 Medical Faculty of the Military Medical Academy, University of Defense, Serbia 3 Institute for Physiology and Biochemistry, Faculty of Biology, Serbia Nitric oxide (NO) is an important mediator of inflammatory reaction. Our previous studies showed increased production of NO at the wound site after burn injury. At the same time we found decreased adhesiveness and apoptosis rate of inflammatory granulocytes (Gr) ex vivo and in cultures. The aim of this study was to investigate the influence of locally originated NO on adhesion and apoptosis of inflammatory Gr at the wound site after burn injury. Inbred Albino Oxford rats were subjected to non-lethal, full-thickness burn injury. Immediately after injuring, sterile polyvinyl sponges were implanted subcutaneously on the borderline of the burned skin in order to obtain inflammatory Gr. Control group were animals implanted with sterile sponges only. In vivo application of aminoguanidin (AG), a specific inhibitor of inducible nitric oxide synthase (iNOS), resulted in increased apoptosis rate of inflammatory Gr from control animals, while this treatment was ineffective in burn injured animals. In regard to Gr adhesiveness, in vivo AG treatment had an opposite effect manifested by stimulation of inflammatory Gr adhesion from burn injured rats, while adhesiveness of Gr from control animals was unchanged. Cumulatively, these results indicate that locally produced NO contribute to the inhibition of Gr adhesiveness without significant influence on apoptosis rate of these cells at the wound site after burn injury. Acknowledgements This study was supported by the project of Ministry of Defense of the Republic of Serbia (MFVMA/4/12-14). Keywords: Granulocytes, injury, Nitric Oxide, Adhesion, Apoptosis Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Draskovic-Pavlovic B, Vucevic D, Majstorovic I, Bozic B, Zunic G and Colic M (2013). Different role of nitric oxide on adhesiveness and apoptosis of inflammatory granulocytes after burn injury. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00200 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Biljana Draskovic-Pavlovic, Institute for Medical Research Military Medical Academy, Belgrade, Serbia, biljadp@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Biljana Draskovic-Pavlovic Dragana Vucevic Ivana Majstorovic Biljana Bozic Gordana Zunic Miodrag Colic Google Biljana Draskovic-Pavlovic Dragana Vucevic Ivana Majstorovic Biljana Bozic Gordana Zunic Miodrag Colic Google Scholar Biljana Draskovic-Pavlovic Dragana Vucevic Ivana Majstorovic Biljana Bozic Gordana Zunic Miodrag Colic PubMed Biljana Draskovic-Pavlovic Dragana Vucevic Ivana Majstorovic Biljana Bozic Gordana Zunic Miodrag Colic Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.