In a field experiment on barley at Rothamsted with the high mean yield of 49 cwt. of grain per acre, the varieties Proctor and Herta produced 10—15 per cent, more grain than Plumage-Archer on plots that received no nitrogenous fertilizer. When nitrogen was applied the difference was increased to about 30 per cent., because the higher nitrogen supply caused the Plumage-Archer crop to lodge and did not increase its yield, while Proctor and Herta remained standing. The three varieties did not differ in leaf-area index nor in net assimilation rate before ear emergence, so that all had the same total dry weight. After ear emergence, the leaf-area indices of Proctor and Plumage-Archer were nearly equal, but that of Herta was smaller. Assuming that the photosynthetic efficiency of the leaves continued to be the same in all varieties, the higher grain yields of Proctor and Herta cannot be attributed to greater production of dry matter by the leaves, either before or after ear emergence. A pot experiment on plants with shaded ears confirmed that the dry matter contributed to grain yield by unit leaf area was nearly equal in all the varieties. The higher grain yield of Proctor and Herta than of Plumage-Archer must therefore have come from additional photosynthesis in parts of the plant other than the leaves, i.e. in the ears themselves. An attempt to demonstrate this directly in a pot experiment, by comparing the grain yields of plants with shaded or with unshaded ears, was unsuccessful because the varieties behaved differently in pots; Proctor and Herta produced only about 6 per cent, more grain yield than Plumage-Archer, and though the decrease in grain yield by shading the ears was slightly greater for Proctor and Herta, the differences were not significant. The sum of ear sizes (estimated from length and breadth measurements) per m.1 in the field experiment was greater for Proctor and Herta than for Plumage-Archer. Also the distribution of dry matter between developing ears and shoots apparently differed with variety, so that at ear emergence the dry weight of ears per m.2 was greater in the two higher yielding varieties. The increased amount of photosynthetic tissue in the ears of Proctor and Herta, as measured by size or weight, may not wholly explain their greater dry-matter production; ears of Herta may also have a higher photosynthetic efficiency. No differences in nutrient uptake that could account for the varietal differences in grain yield were found. Plumage-Archer absorbed more potassium, and Herta less phosphorus than the other varieties. About a quarter of the final content of nitrogen, and a third of the phosphorus, was absorbed after ear emergence, but the potassium content was nearly maximal at ear emergence and later decreased. The pot experiment showed that, on the average of all varieties, 26 per cent. of the dry matter in the grain at harvest originated from photosynthesis in the ears, including 10 per cent, from the awns; 59 per cent, came from photosynthesis in the flag-leaf lamina and sheath and peduncle, and 15 per cent, from parts of the shoot below the flag leaf.