Pearl millet grains were characterized in terms of moisture, viscosity, Fourier transform infrared (FTIR) and near infrared (NIR) spectroscopic analysis. The moisture content was determined using Association of Official Analytical Chemists (AOAC) standard method, viscosity of grains was determined via Labman 200 rotational viscometer. In this study, the data was collected for the pearl millet grains and analysed using Fourier Transform Infrared Spectroscopy (FTIR) and Near Infrared Spectroscopy (NIR). The average value of moisture and viscosity was 16.26% and 1769.22 mPaSec, respectively. The fourier transform infrared analysis showed the presence of N-H and O-H compounds from pearl millet. The analysis from near infrared spectra of the pearl millet grains showed the N-H stretching, O-H stretching and C-H stretching first-overtone and combinational bands.