Gradual Reconstruction Research Articles

Is transposed skin transformed in major head and neck mucosal reconstruction?

We used histological and histochemical methods to investigate the possible changes with time in skin transposed into the oropharynx and oral cavity of patients whose surgical wounds were reconstructed with the classic pectoralis major flap. We found histological evidence that skin morphology was maintained (keratinization with reduced, but the desquamating layer, hair follicles, sweat and sebaceous glands were identifiable), and noted an intense inflammatory reaction in the dermis. Histochemical studies confirmed the progressive flattening of the basal interdigitations between the epithelium and dermis, and the gradual reconstruction of the basement membrane. Although skin-mucosa continuity developed fairly quickly without scar reactions, the two epithelia maintained their original patterns of keratin production, i.e., their textural peculiarities. We propose an original method for the transposition of muscular flaps implanted with autologous culture of keratinocytes, an approach developed in an attempt to ensure more satisfactory rehabilitation of the removed mucosal structures.

Seveso Oak forest: the gradual reconstruction of an area contaminated by TCDD: P. Lassini, P. Ballardini, A. Mambriani & F. Monzani, Landscape & Urban Planning, 23(3–4), 1993, pp 221–231

Seveso Oak forest: the gradual reconstruction of an area contaminated by TCDD: P. Lassini, P. Ballardini, A. Mambriani & F. Monzani, Landscape & Urban Planning, 23(3–4), 1993, pp 221–231

Seveso Oak Forest: The gradual reconstruction of an area contaminated by TCDD

Located to the north of Milan, is the Castanetum area which is densely populated and is the site of numerous companies, industrial plants, farms and forests. In 1976 a toxic cloud of dioxin (TCDD) seriously contaminated the area and after 5 years an international commission decided to decontaminate it, by removing about 40 cm of the top layer of the contaminated soil and placing it in two special dumps. In 1984 the decontamination process was concluded, a project for the park was created and a private contracting firm began construction; the Regional Forest Agency also began its collaboration in this project with the supervision of the reforestation by Dr. Paolo Lassini. After a series of technical and functional problems that were resolved through the intense, concerted efforts of technicians, various government organizations and universities, the park was completed in 1986. Nevertheless, this was just the beginning of a barely roughed-out forest ecosystem made up of grass lawns, about 5000 grown trees and 10 000 bushes. A building, an irrigation system, a road and fencing are the only constructions in the park. In 1987 the management of the park was turned over to the Regional Forest Agency, under the technical direction of Dr. Paolo Lassini. Work began with a group of collaborators and specialized employees of the RFA, who were also trying to integrate the forest ecosystem with the surrounding landscape. In the past 5 years RFA has accomplished the following things: • -increased the natural resources through reforestation — the planting of bushes, full-grown plants, large trees, plant specimens and the planting of undergrowth and a second generation of plants, bushes and trees; • -created wildlife areas and reintroduced various forms of wildlife; • -created marsh areas; • -created ornamental garden areas; • -created acoustical barriers; • -created park areas linked to the neighbouring districts; • -established contacts and initiatives with environmental and wildlife protection groups, fishermen and hunters associations; • -in collaboration with the University, created inventories to carry out studies on the flora and fauna. The following are some of the initiatives under examination for the next few years: • -creation of a larger regional park including neighbouring areas; • -wildlife areas for teaching; • -creation of a compost centre.

Reconstruction of the basement membrane in a cultured submandibular gland

In a rat submandibular rudiment on day 16, both laminin (LM) and type IV collagen (Col-IV) were found in all cases to colocalize not only in the basement membrane, but also in the rough endoplasmic reticulum of the epithelial cells, indicating that the synthesis of the components of basement membrane is greatly enhanced at this particular stage of extensive branch formation. Using the submandibular gland from a 16-day embryo, the model system was developed to determine the structural organization of the basement membrane. The pre-existing basement membrane was digested with collagenase and dispase, causing its complete disappearance. The subsequent gradual reconstruction of an authentic basement membrane was confirmed by electron microscopy and immunohistochemistry of LM and Col-IV. In the model system, this recovery started at 4 h of culture, and formation was complete by 8 h. During the recovery, thick bundles of actin filaments appeared transitionally in the basal cytoplasm. Electron microscopic analysis indicated two precursor structures, aggregated fuzzy fibers (type 1 extracellular matrix (ECM)) and 10-nm-thick strand piles (type 2 ECM), and an authentic basement membrane structure appeared during the course of membrane reconstruction. LM and Col-IV were always located together in these three structures. These observations clearly indicate that the precursors, containing LM, Col-IV and most likely heparan sulfate proteoglycan, appeared to form immediately following their secretion into the extracellular space, and assembled into the rigid structure of basement membrane within 8 h. The ultrastructural and immunohistochemical process of basement membrane reconstruction appeared to coincide closely with that of the glomerular basement membrane in developing kidney.(ABSTRACT TRUNCATED AT 250 WORDS)

Variazioni delle popolazioni di microfunghi del suolo durante il post-incendio di Monte Argentario

Abstract Post-fire sequences in microfungal soil populations in Mount Argentario (Toscana region). A research program was conducted to investigate the post-fire sequences of microfungal populations in Thyrrenian Coastal soils. This paper reports the results of the first year of that study. An initial reduction of fungi was followed by a gradual reconstruction process characterized by a constant increase of colonies and species. The periodic after fire analysis permitted an initial understanding of the possible selective role of the fire and its consequences on some microfungal species.

Industrial Changes and Working Intention in Postwar Japan

Few things more actually proved the truth of this law than Japanese industrial society after the World War II. I shall follow the trace of working intention changes, from the period immediately after the last war when food living was in imminent danger to the paresent time when people became to be able to enjoy culture life to some extent, analysing the ten years into five part periods.The first period : 1945-1946Animal-like, food-wishing period. In this period true working intention (will to work) did not exist and only need for existance-egoistic desire to eat-prevailed.The second period : end of 1946-end of 1947The period when human and cultural demand began to recover and sprout. In this period demand progressed from animal grade to human one. The signs of recovery of working intention began to appear with gradual reconstruction of industrial world, but demand for higher wages in compensation for labor was exceedingly egoistic.The third period : 1948-1950The period when requirement for working conditions as well as for wages began to be conscious of. In this period the improvement of working conditions and requirements regarding adjustment of the social conditions in the workship together with for wages was considered, according to gradual recovery of industrial world and worker's living standard.The forth period : 1951-1952The period when requirement for inherent conditions of labour was generalized. In this period demand for higher wages did not decline as before and inherent conditions of labour and requirement for adjustment of Human Relation, Job Relation etc. were strengthened and proposition of improvement for them was generalized.The fifth period. 1953-The period when requirement for rational distribution and persuit for other profit besides wages began to be considered. Because of standstill of Japanese industry the problem of base-up became impossible or hopeless, so demend for higher wages rised up again and wishes for inner rationality and other profit besides wages appeared.Saying in other words, workers require their reasonable wages in compliance with their labor accomplishments, and on the other hand their hand they tried to acquire the stability and elevation of their living. That the requirement for public welfare is rapidly rising endorses this tendency.As above mentioned we may say that working intention after the world War II was chiefly influenced and ruled dy industrial, social conditions and pre-modern, animal-like wishes for existance. In this sense the present state of working intention in our industrial world does not yet come back to a satisfactory situation. Therefore, thouge workers require rational distribution, still they wish in another way much more distribution, neglecting their accomplishments, and expect their fleeting enjoyment of profit in common by way of prevention for a standing manace to their living.Here, we notice a strain, abnormalism of working intention in Japan industrial world, and importance, difficulty of working intention control.