The Gradient Richardson Number (Ri) is an important parameter for appraising the stability and turbulence exchange at the atmospheric boundary layer (ABL). However, high-resolution measurements of Ri profiles are rarely reported, especially in megacities. In this study, a Doppler wind lidar and a microwave radiometer were simultaneously utilized to measure the 2 km Ri vertical profile in downtown Beijing. These measurements were verified to have high accuracy compared with observations from a 325 m meteorological tower, with root-mean-square errors (RMSEs) of less than 1.66 K, 7.9%, and 1.45 m/s for the temperature, relative humidity, and wind speed (WS) for all altitudes and corresponding Pearson correlation coefficients (R) of 0.97, 0.93, and 0.81. The inter-comparisons of different spatial (25 m, 50 m, 100 m) and temporal resolutions (1 min, 30 min, 1 h) form a 3 × 3 resolution matrix of Ri, in which the 1 h temporal resolution of Ri overestimates the intensity and active area of turbulence. The Ri value retrieved from the 100 m spatial resolution data overestimates these by half as it misidentifies the height of the stable area at the near surface. There are significant differences between the data with a 1 min temporal resolution and a 25 m spatial resolution (defined as the standard resolution of Ri), and the rest of the data in the resolution matrix (defined as data at other resolutions), with an RMSE > 1 and an R < 0.8. The difference between data at the standard resolution and data at other resolutions increases with elevations, which results from frequent weather processes or from water-vapor blocking at higher altitudes. The Ri profiles reveal that the atmospheric layer at altitudes from 100 m to 500 m in daytime is unstable, with Ri < 0, while it is neutral, with 0 < Ri < 0.25, at night-time from 200 m to 400 m. The atmosphere above the ABL in a megacity is rather stable, with Ri > 0.25, whereas below the ABL, it is neutral or unstable, which is due to drastic changes in the WS and temperature that are affected by the topography and surface friction.