GPT Levels Research Articles

Comparative hepatoprotective effects of flavonoids-rich fractions from flowers and leaves of Penthorum chinense Pursh in vitro

Ethnopharmacological relevancePenthorum chinense Pursh is a traditional Miao ethnomedicine rich in bioactive components, widely recognized for its hepatoprotective properties. However, the hepatoprotective effects of its flowers and leaves have not been individually elucidated. Aims of the studyThe objective of this study was to isolate and purify flavonoids-rich fractions from the flowers (PFF) and leaves (PLF) of P. chinense, and to assess their potential protective effects against oxidative, alcohol-induced, and free fatty acid (FFA) induced injury in hepatic cells. Materials and methodsThe P. chinense flowers and leaves flavonoids-rich fractions were extracted by the method optimized by response surface methodology, and the extracts were subsequently purified using petroleum ether and microporous column. The physical characteristics and component composition of PFF and PLF were analyzed by FT-IR and UPLC-MS/MS. The hepatoprotective activities of PFF and PLF were evaluated by the alcohol, H2O2, and FFA-induced hepatocyte injury cellular model in vitro. The protective effects of PFF and PLF on the hepatic cells were evaluated by assessing cell apoptosis rate, enzymes activities, mitochondrial membrane potential, and mRNA expression in relevant signaling pathways. ResultsThe results revealed that PFF was mainly composed of pinocembrin, quercitrin and quercetin, while PLF was predominantly composed of quercetin, pinocembrin, and kaempferol and their derivatives. PFF and PLF exhibited distinct effects on increasing the cell proliferation rate, regulating the MDA, GOT and GPT levels, and modulating the mRNA expression in apoptosis and antioxidant pathways in alcohol damaged LO2 cells. PFF exhibited superior efficacy in reducing cell apoptosis in alcohol-damage cells compared to PLF. Both PFF and PLF alleviated mitochondrial stress in H2O2-induced LO2 cells. Additionally, the PFF and PLF attenuated lipid accumulation and activated mRNA expressions in PPARα/ACOX1/CPT-1 lipid metabolism pathways, as well as Nrf2/ARE oxidative stress pathways. ConclusionThis study compared the hepatoprotective activities of flavonoids-rich fractions purified from the flowers and leaves of P. chinense. The results contribute to the enhanced development and utilization of various parts of P. chinense aimed at medical and health food applications.

Estimating the Biological Variables of Thyroid Patients and Their Impact on Diabetics in Kirkuk

Background: Investigating the impact of sugar on biochemical variables in patients with thyroid dysfunction in Kirkuk, including some previously unmeasured biological variables. Materials and Techniques: From November to May, this study occurred in Kirkuk. Blood samples were collected from 120 patients aged 20 to 80 years, including 40 diabetics, 40 with both hyperthyroidism and diabetes, and 40 with both hypothyroidism and diabetes, as well as 40 healthy individuals aged 20 to 40 without chronic diseases. Levels of TSH, T4, T3, HbA1c, GPT, GOT, ALP, and HBs were measured in the serum using Beckman Coulter Access2, Spectrophotometer, and Boditech Med INC I-Chroma AFP-25 devices. Results: The study found that TSH levels were significantly elevated in patients with diabetes and hypothyroidism (P≤0.01), while T4 and T3 levels were significantly decreased (P≥0.01). HbA1c levels showed a significant increase (P≤0.01) in all groups: diabetes with hypothyroidism, diabetes with hyperthyroidism, and diabetes alone. Enzyme levels (GOT, GPT, ALP, HBs) were also significantly elevated (P≤0.01) in these groups. Conclusion: In conclusion, TSH regulation is vital, with high levels indicating hypothyroidism related to obesity and diabetes. Elevated HbA1c shows poor glucose control, and hyperthyroidism with diabetes worsens liver function and glucose metabolism, stressing the need for integrated management.

A Comparative Study of Biomarker Levels for Iraqi Individuals’ Smokers and Non-Smokers Depending on the Duration of Smoking

Smoking is a widespread public health challenge globally, impacting societal health and economy while elevating the risk of cardiovascular and peripheral vascular diseases. This study aims to examine hematological parameters (HB, PCV%, WBC, RBC, PLT, ESR), liver and kidney function, lipid profile, and electrolytes (K+, Na+, Ca++) in male smokers aged 20-55 from Baghdad City, subdivided by smoking duration (≥5 years and <5 years), compared to non-smoking controls. A total of sixty participants were recruited across three areas (Diyala Bridge, Mada'in, Talbieh), with each group comprising thirty individuals. Venipuncture was performed for blood sample collection. Results revealed statistically significant elevations in total WBC and RBC counts, hematocrit, hemoglobin, total cholesterol, and liver enzymes (GOT, GPT, ALP) among smokers compared to non-smokers (p<0.05 or p<0.001). Serum uric acid, urea, and creatinine levels were significantly higher in smokers (p<0.001), while triglyceride levels showed no significant difference (p>0.05) between groups. Smokers with longer duration (>5 years) exhibited higher HB, triglyceride, and Na+ levels compared to shorter-term smokers. Sensitivity analysis indicated Na+ as a potentially effective biomarker for smoking-related health impacts, complementing PCV% and urea sensitivity. The study underscores the association between smoking and oxidative stress, notably reflected in elevated GOT and GPT levels, suggesting implications for antioxidant therapies in managing smoking-induced oxidative damage. These findings contribute novel insights into biomarker responses influenced by smoking duration and advocate for targeted public health strategies and further research into antioxidant interventions to mitigate smoking-associated health risks effectively.

A PET/MRI study on the effect of obesity and NAFLD on hepatic [18F]FDG uptake

PurposeThe potential limitations of hepatic [18F]FDG-PET imaging for individuals with obesity and excessive liver fat (NAFLD) are being investigated. In this study, we aim to determine the reliability of standardized uptake values (SUVs) focusing on adjustment for liver fat content (LFC) derived from DIXON images and the effects of whole-body normalizations. MethodsLean and with obesity volunteers who underwent [18F]FDG-PET/MRI were reviewed retrospectively. DIXON fat images were used to determine LFC and for adjustment of SUVmean. The hepatic SUVs (mean, fat adjusted mean and max) were normalized to body weight, lean body mass and body surface area. Blood samples were analysed for glucose, serological liver enzymes and lipoproteins for further correlation of [18F]FDG uptake. ResultsOut of 11 volunteers with obesity (M:8, F:3, BMI:30–39 kg/m2), 9 confirmed the presence of NAFLD (>5.6 % fat). 22 age-matched lean volunteers (M:10, F:11, BMI:19–26 kg/m2) were used as control group. Both SUVmean, before and after adjustment to LFC, did not provide any difference between lean and with obesity groups under BW, LBM and BSA. SUVmax BW showed a difference between groups (p = 0.05). SUVs were independent of levels of GPT, GOT, gGT, insulin, HOMA-IR, triglycerides, cholesterol and LDL. Volunteers with low HDL were clustered with an increased hepatic [18F]FDG uptake. ConclusionOur method for adjustment of hepatic [18F]FDG-PET with DIXON fat images allows to achieve accurate results for individuals with NAFLD and obesity. For homogenic results, raw SUVmean should be combined with adjustment for liver fat, appropriate normalization and consideration of HDL levels.

Impact of Amphetamine and Captagon abuse on the liver and renal function

Background. Addiction is a multifaceted brain disorder characterized by compulsive substance use despite harmful consequences. The physiological effects of drugs, such as amphetamines and captagon, on organ functions remain a significant concern, with varying impacts based on substance type and duration of use. Aim of study. This study aimed to investigate the impact of amphetamine and captagon abuse on liver and kidney functions. By comparing physiological parameters between individuals with substance abuse history and a control group, the study sought to elucidate the health implications of long-term drug use. Methods. The study involved 50 participants: 30 with a history of substance abuse and 20 healthy controls. It assessed liver and kidney functions using biochemical and ELISA tests to measure AST, ALT, ALP, Total Serum Bilirubin, Serum Albumin, Serum Creatinine, and Blood Urea. Results. The study revealed a significant difference between drug users and controls, with drug users exhibiting higher levels of urea and serum creatinine. Liver function tests showed increased GOT, GPT and ALP levels in drug users. Furthermore, total serum bilirubin was significantly higher in drug users, indi­cating liver impairment. Age-related analysis showed more severe renal and liver dysfunction in older drug users, with notable increases in urea and serum creatinine levels with age (p=0.001 and p=0.009, respectively). Conclusion. Both amphetamines and captagon pose a significant health risk to renal and liver functions. This underscores the need for awareness and interventions targeted at mitigating the health implications of substance abuse, highlighting the importance of continuous monitoring and treatment strategies for affected individuals.

Profiling of two Lampranthus species using LC-ESI/MS with evidence of their hepatoprotective activity

Lampranthus glaucus and Lampranthus glaucoides are only reported to have significant cytotoxic activity against certain cancer cell lines with phytochemical investigation of their petroleum ether and the ethyl acetate extracts. Further investigation was suggested concerning their hepatoprotective activity and relating it to the metabolic profile of their defatted methanol extracts using LC–ESI/MS analysis. Hepatoprotective activity was evaluated through assessment of three liver parameters as well as liver histopathological examination in thioacetamide-induced hepatotoxicity model. Sixty-eight and 26 phytochemicals were tentatively identified in L. glaucoides and L. glaucus, respectively, with phenolic compounds as the major class. Both plants showed significant inhibition of serum GPT and GOT levels, inhibition of tissue IL-1β and TNF-α levels and inhibition of tissue NF-κβ and caspase-3 gene expression proving hepatoprotective action. Liver treated with L. glaucoides showed lesion scoring range between negative to mild, whereas L. glaucus showed a range between mild to moderate.

Mechanisms of the TGF-β1/Smad3-signaling pathway in gender differences in alcoholic liver fibrosis

The TGF-β1/Smad3-signaling pathway and gender differences were investigated in alcoholic liver fibrosis. Mice were divided into female normal, female model, male normal, and male model groups. Liver injury and fibrosis were assessed using histopathology and serology. Western blotting was performed to analyze the expression of relevant factors. HSC-T6 cells were divided into estradiol + saline, estradiol + ethanol, testosterone + saline, and testosterone + ethanol groups, and similar assessments were conducted in vitro. Compared with the female model group, the male model group exhibited significantly increased GPT, GOT, TNF-α, IL-6, and testosterone levels, fibrosis rate, and TGF-β1, Smad3, and PCNA expression, and significantly decreased estradiol levels and Caspase-3 expression. The apoptosis rate was higher in the estradiol + ethanol group than in the testosterone + ethanol group, although the testosterone + ethanol group exhibited significantly increased TNF-α, IL-6, Collagen-I, α-SMA, TGF-β1, Smad3, and PCNA expression, and significantly decreased Caspase-3 expression. Alcoholic liver fibrosis showed significant gender differences associated with the TGF-β1/Smad3-signaling pathway.

PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST INDIAN PAPER WASP ROPALIDIA MARGINATA VENOM TOXINS AND THEIR EFFICACY IN THE REVERSAL OF TOXIC EFFECTS

Objective: In this study, albino mice were injected with a sub-lethal dosage of purified wasp Ropalidia Marginata venom toxins to assess the effectiveness of polyclonal anti-venom antibodies. Methods: To neutralize the toxic effects, polyclonal antibodies were generated by immunizing albino mice. The antibody underwent partial purification using ammonium sulphate treatment and octanoic acid precipitation. To detect the presence of antibodies in the antiserum, an immunodouble diffusion test was conducted using Ouchterlony's method (1962). This involved allowing both antigens and antibodies to diffuse radially towards each other from their respective wells. When they reached an equivalence zone, a precipitation complex of antigen and antibody became visible as a concentric band, indicating the development of the combination. To quantitatively determine the amount of antibodies in the antiserum, the equivalency zone approach was used. Results: Experimental mice were injected with a combination containing 400, 800, and 1200 µg of pure antibody, which had been treated serum biomolecules, including metabolic enzymes, completely reversed in the experimental with 40% of the LD50 of wasp venom the elevated serum parameters were glucose, pyruvic acid, lipid, protein and free amino acid, reached to normal (100%) in the treated with 40% of LD50 of the venom and polyclonal treated after 6 h of administration. Anti-serum treatment also successfully normalized the alteration in serum enzyme just after 4h. Similarly, anti-serum treatment also successfully normalized the alteration in serum enzyme just after 4h treated with 40% of LD50 of the venom. Serum ACP content was obtained as 125.35% after 40% of LD50 venom injection, which was get normalized up to 102.81% after 4 h of the anti-venom treatment. Serum ALP content of 114.8% elevation was reversed back to 102.40% after anti-venom treatment. The GPT level significantly reversed up to 102.5%, while it was 130% in the venom-treated mice. A complete reversal was obtained in GPT level, which was obtained as 104.54% in the venom-treated animal. Similarly, LDH which was elevated up to 112.45 % in venom-injected mice was successfully reversed up to 100.25% after anti-venom treatment. Similarly, Ache concentration was fully recovered after anti-venom treatment 6 h, all animals (group B-E) that had received 40% of the LD50 of venom treated with pure antiserum. Conclusion: The venom-injected group showed a complete restoration of serum protein, free amino acid, uric acid, cholesterol, pyruvic acid, total lipid, and glucose level in experimental mice.

Assessment of Toxicity of Green Synthesized Silver Nanoparticle-coated Titanium Mini-implants with Uncoated Mini-implants: Comparison in an Animal Model Study.

To assess the potential for systemic toxicity when silver nanoparticle-coated mini-implants were implanted in Wistar albino rats conducted as a comparative study in the animal model by assessing the blood biochemistry, liver and kidney function, and histology of the implanted site. The surface of the mini-implant was coated with a green-mediated silver nanoparticle. Uncoated mini-implants were placed in two groups of eight Wistar albino rats, and silver nanoparticle-coated mini-implants were placed in another eight rats. The bone's general conditions, blood biochemistry assessing for ALT, AST, GPT, GOT, and histological sections using H and E stain and Masson's Trichrome stain were examined at 7, 14, and 28-day intervals. The creatinine, urea, ALP, and ALT showed no signs of systemic toxicity during the 28-day follow-up period in the Wistar rats both in the test and control groups. The histological evaluation, which was conducted using HE and MTS stain, revealed osteogenesis and adequate healing of the insertion site in the group where coated mini-implant was placed. The bone sample revealed no abnormalities in the control group with uncoated mini-implants. Green synthesized silver nanoparticle-coated mini-implant does not cause systemic toxicity as indicated by no abnormalities in the levels of creatinine, urea, ALT, ALP, GPT, and GOT. The bone histology indicates that the coated mini-implants placed in animal bone healed with adequate osteogenesis. Silver nanoparticles have potential for antimicrobial activity. Mini-implants placed as temporary anchorage devices in orthodontics often fail due to inflammation and plaque. Silver nanoparticle-coated mini-implants would reduce the risk of mini-implant failure as it would have antimicrobial potential and eliminate this cause for failure of mini-implants. How to cite this article: Sreenivasagan S, Subramanian AK, Mohanraj KG, et al. Assessment of Toxicity of Green Synthesized Silver Nanoparticle-coated Titanium Mini-implants with Uncoated Mini-implants: Comparison in an Animal Model Study. J Contemp Dent Pract 2023;24(12):944-950.

Combined quercetin and simvastatin attenuate hepatic fibrosis in rats by modulating SphK1/NLRP3 pathways

Liver fibrosis involves several signalling pathways working in concert regulating the deposition of extracellular matrix. In this study, we evaluated the effect of quercetin and simvastatin alone and their combination on the treatment of experimentally induced hepatic fibrosis in rats. To decipher the potential mechanisms involved, liver fibrosis was induced in rats by administration of 40 % carbon tetrachloride (CCl4) (1 μl/g rat, i.p., twice weekly) for 6 weeks. Quercetin (50 mg/kg, orally), simvastatin (40 mg/kg, orally) either individually or combined were administered for another 4 weeks. The three treatment groups ameliorated hepatic dysfunction and altered parameters of sphingolipid and pyroptosis pathways. Yet, the combined group showed a more pronounced effect. Treatments lowered serum levels of GOT, GPT, ALP and elevated albumin and total protein levels. Histopathological and electron microscope examination of liver tissue revealed diminished fibrosis and inflammation. Protein expression levels of α-SMA, IL-1β, PPAR-γ, TGF-β1, caspase-1 and caspase-3 expression in liver tissues were reduced. Additionally, hepatic mRNA levels of SphK1 and NLRP3 decreased after treatment. Furthermore, the three groups lowered MDA levels and elevated total antioxidant capacity, GSH and Nrf2 expression levels. Treatments downregulated sphingolipid pathway and NLRP3-mediated pyroptosis and stimulated an anti-apoptotic, anti-proliferative and antioxidant activity. This suggests that targeting the SphK1/NLRP3 pathway could be a prospective therapeutic strategy against liver fibrosis.

Synergetic Role of Energy and Oat with Enzymes on Physiological Performance of Broiler

This study was conducted in poultry fields at College of Agriculture / University of Anbar’s Animal Production Department from 2/9/2022 to 14/10/2022 (42 days). This study examined the physiological effects of high energy and oats on grill performance. Three hundred one-day-old (unsexed) chicks were distributed in a completely randomised design to ten treatments with three replicates (10 chicks per replicate). All diets were designed to meet the same requirements. Broilers received water and feed ad libitum. Treatments were as follows: T1 birds fed diets for three phases (starter, grower, and finisher) without any addition, T2 birds fed diet (starter 3100 Kcal /kg feed) + 5% oat + 0.01 mg multienzyme, T3 birds fed diet (starter 3000 Kcal/kg feed) + 5% oat + 200 mg multienzyme, T4 birds fed starter 2900 Kcal/kg feed + 5% oat + 200 mg multienzyme, T5 birds fed diet (grower 3200 Kcal/kg feed) + 5% oat + 200 mg multienzyme, and T6 birds fed diet The 5% oat hull groups had higher small intestine villus height and CD than the control group (p < 0.05). T6 group glucose is higher than control group glucose. T10 group and control group total protein differ significantly (P>0.05). The control group’s albumin, MDA, and GPT levels are not significantly different from the other groups. Dietary oat hull increased Glutamic-Oxaloacetic Transaminase (GOT) activity in T6 compared to control group, but control group had higher ALP activity.

Effects of mid-to-late prepartum feed supplementation in Hanwoo beef cows on their performance, blood metabolites, and the carcass characteristics and metabolites of their neonatal calves

IntroductionThis study aimed to evaluate the implications of supplementary nutrition during the mid-to-late pregnancy on various parameters in Hanwoo cows and their subsequent neonatal calves.Materials and methodsEight Hanwoo cows in their first parity were divided into two groups. The control group (C, 100%) received 3kg of concentrate and 5kg of rice straw throughout the pregnancy period, while the treatment group (T, 150%) increased their diet during mid-to-late pregnancy. Both performance assessments and blood metabolite analyses were performed for the pregnant cows. Neonatal calves were subjected to morphometric evaluations, blood sampling, and detailed morphometric analyses of carcasses and gastrointestinal components.ResultsPerformance indices of the cows showed that both Pregnancy Period (PregP) and Body Condition Score (BCS) were significantly improved with supplemental feeding (p &amp;lt;0.05). Improvements in Body Weight (BW) and Feed Conversion Ratio (FCR) were not statistically significant. Blood metabolite analysis for the cows revealed decreased levels of triglycerides (TGLate), non-esterified fatty acids (NEFALate), and progesterone (P4Late), with a notable increase in glucose (GluLate) levels (p &amp;lt;0.01). In the neonatal calves, anatomical metrics of the gastrointestinal tissues showed increased Omasum Width (OmasWdth) values in the supplemented group (p =0.053). There was significant increase of papillae and villus lengths in the rumen and small intestine (p &amp;lt;0.01 and p &amp;lt;0.05, respectively). Morphometric evaluations displayed longer body lengths (BLnth) and larger chest width (ChestWdth) in the treated calves (p &amp;lt;0.05 and p &amp;lt;0.01, respectively). Carcass characteristics showed no substantial variations between the groups, while blood analysis in the calves revealed decreased GPT levels in the nutritionally supplemented group (p&amp;lt;0.05).DiscussionThe findings indicate that supplementing the diets of Hanwoo cows during mid-to-late pregnancy leads to significant changes in select maternal blood metabolites and influences specific anatomical and morphometric features in neonatal calves, all without significant shifts in carcass attributes.

Comparative analysis of the anti-depression effects of lorazepam, acupuncture, and curcumin utilizing the forced swimming test in experimental rats models

Introductionlorazepam is one of benzodiazepine medication that is usually used for its sedative and anxiolytic effect and has anti-depressant like effects. Turmeric is a plant that contain curcumin as main active compound that exert its antidepressant action inhibit monoamine oxidase and increase levels of dopamine and serotonin in brain. Aimthe present study investigate the depression effect of curcumin (natural selective serotonin inhibitors) in comparison with acupuncture and lorazepam. Method32 adult rats weighing (150 g−280 g) were divided equally into 8 groups each group contain 4 rats. The first 3 group of rats were treated with lorazepam, mixture of (curcumin, black pepper and olive oil) and pricked with insulin needle at point gb39, respectively, while second 4 groups of rats were received lorazepam and mixture, lorazepam and acupuncture, mixture and acupuncture, and lorazepam, mixture and acupuncture, separately, and the eighth group is control group don't receive any drugs. After 35 days, forced swimming test was made for all rats. ResultsLiver parameters: - GOT increased in all group, GPT increased in rat's group that received lorazepam, mixture group and lorazepam+ mixture group, This indicates the existence of problems previously with the liver of the rat used, and what remains is the high levels of GOT and GPT in the control group, while TSB is within the normal range for all groups. Kidney parameters:-blood urea levels increased in all groups and S. creatinine levels within normal range for all groups. Conclusionthe evaluation of natural SERMs in the forced swimming test showed promising antidepressant-like effects, comparable to lorazepam and acupuncture. Further research is needed to understand the mechanisms and determine optimal dosage and safety. Natural selective serotonin inhibitors hold potential as alternative or complementary treatments for depression.

Evaluation of liver enzymes activity and some trace elements in sickle cell anemia patients in Basrah Governorate – Iraq.

Sickle cell anemia is one of genetic diseases caused by hemoglobin biochemical disorder in especially in polypeptidic chain of this protein. The study was conducted from October 2019 to December 2020 and included two sets of samples Patient and Healthy .The current study was curried out to evaluate some enzymes and trace elements in patients with sickle cell anemia. The levels of copper (Cu) were estimated spectrophotometrically by reaction of copper with 4 – (3,5 – dibromo – 2- pyridylazo) – N – ethyl sulphopropylaniline to produce a chelating complex.The determination was carried out at wavelength equal to 520 nm. Finally, absorbances were recorded and they are proportional to concentration of copper in the sample . Also, the levels of zinc in serum of blood belonging to sickle cell anemia (SCA) were investigated and estimated by reaction of zinc with chromogen existing in the characteristic kit to produce coloured complex. The absorbances were recorded spectrophotometrically which are proportional to concentration of zinc in blood sample . The levels of biochemical variables were estimated using ARCHITECT c4000 systems provided by ABBOTT Company, which are designed with advanced technology, accuracy in clinical results and ease of handling. The device uses a sample size at a rate of 6µL in all measurements of chemical variables.The total values of the current results were made and expressed by calculation of the mean ± standard deviation. Analysis of all results data were carried out by using a statistical procedure according to analysis of variance univariate . Statistical programmer of social science (SPSS ,v.23 ) was depended for calculation of significant differences for the different groups of patients infected by SCA disease and healthy persons . The correlation regression coefficient (r) was used and depended to distinguish between the means of groups belonging to all patients and healthy individuals ( control group ) .The p-value was agreed for less than 0.05 to the limit of lowest significance . A highly significant difference was recorded belong to levels of GPT, ALP and GOT in patients compared with healthy individuals (control group) according to age and sex factor.

Toxic effects of perfluorocaproic acid (PFHxA) on crucian carp (Carassius auratus) and the response of the intestinal microbial community

Perfluorocaproic acid (PFHxA), a short-chain substitute for the emerging contaminant perfluorinated compounds, has been detected in the aquatic environment. However, its aquatic toxicity and health risk assessment are mainly unknown. In this study, we compared the toxic doses of 0 mg/L, 5 mg/L, 15 mg/L, 45 mg/L and 135 mg/L on the pathological damage to tissue sections, antioxidant indexes and inflammatory factor expressions in liver, spleen, kidney, Prosogaster, Mid-gut, Hid-gut as well as the changes of IgM, C3, C4, LZM, GOT, GPT in serum of crucian carp. We determined the response of the intestinal microbial community to PFHxA stress by 16S. The results showed that the growth performance of crucian carp was slowed with the increase of PFHxA dose, which caused different degrees of damage to the tissues. Meanwhile, the indexes of SOD, GSH-Px, T-AOC, ACP, AKP and LZM in each tissue were reduced, and the indexes of IgM, C3, C4 and LZM in serum were reduced. The levels of MDA, GOT and GPT in tissues and GOT and GPT in serum were promoted. In addition, IL-1β, TNF-α, NF-KB, and KEAP-1 in each tissue increased compared with the control group. The levels of IL-10, Nrf2, CAT, and GPx were decreased. The 16S rRNA gene sequencing results showed that PFHxA significantly reduced the abundance and diversity of the gut microbiota. It is suggested that PFHxA is likely to cause different degrees of damage to various tissues by disrupting the diversity of the intestinal flora. These results provide insights to facilitate the risk assessment of PFHxA contaminants in the aquatic environment.

Physiological and histological effects of apigenin and luteolin on Cytarabine injected rats

The present study was undertaken to study the effect of apigenin and luteolin on physiological and histological changes in rats treated with cytarabine drugs. Thirty-five albino healthy male adult rats with equal age weighing 250 -300g were enrolled. Rats were randomly divided into seven groups according to the treatment. Group “1” was treated with normal saline and served as the control group. Groups “2,3 and 4” received cytarabine, apigenin, and luteolin respectively, while groups 5, 6, and 7 received a combination of “apigenin + cytarabine”, “luteolin + cytarabine”, and “apigenin + luteolin + cytarabine”, respectively. After one week of treatment, all seven groups of rats were sacrificed for histological finding, and blood samples were collected from each rat for biochemical parameters analysis. The results of this study showed that cytarabine increased the activity of GPT, GOT, and cholesterol levels in rats after one week of intraperitoneal injection in comparison to the control. There was no significant difference in GPT and GOT when apigenin and luteolin were administrated orally compared to the control group. When apigenin and luteolin are combined with cytarabine, they have a protective effect on leakage of liver enzymes GPT and GOT in serum. Apigenin and luteolin histologically protect liver cells from cytarabine damage when administered alone. These findings conclude that apigenin and luteolin have a protective effect on cytarabine side effects on the liver and its function in rats.

Combined treatment with Alhagi maurorum and docetaxel inhibits breast cancer progression via targeting HIF-1α/VEGF mediated tumor angiogenesis in vivo

Breast cancer is a challenging disease and leading cause of cancer death in women. There is no effective agent for metastatic breast cancer after surgery and chemotherapy. Alhagi maurorum (A.m) has been reported to exhibit an anticancer effect on various types of cancer cells in vitro. This study aimed to examine the suppressive effect of A.m alone and combined with docetaxel (DTX) on the breast cancer growth in mice models and the possible underlying mechanisms. In the present study, the mice were inoculated subcutaneously with the injections of 4T1 cells. Then, A.m, DTX, and their combination were administered intraperitoneally. The expressions of β-catenin (β-cat), FZD7, MMP2, HIF1-α, and VEGF A (vascular endothelial growth factor A) were investigated using RT-PCR method. Also, plasma alkaline phosphatase (ALP), alanine aminotransferase (GPT or ALT), aspartate transaminase (GOT or AST), serum creatinine, and urea were examined, and histological analyses of the tissues were conducted. The results demonstrated that A.m (500 mg/kg) combined with DTX significantly decreased the expression of β-cat, MMP2, and FZD7 as compared with the negative control group and monotherapies. Also, the mRNA levels of HIF1-α and VEGF A were suppressed significantly by DTX + A.m (500 mg/kg). Tumor weights and sizes were significantly lower and tumor inhibition rate was significantly higher in the DTX + A.m group. The A.m 500 mg/kg + DTX also suppressed the serum GPT level in tumor-bearing mice and decreased the serum urea level. Taken together, our findings suggest that DTX combined with A.m at an optimal dose of 500 mg/kg as the optimal dose can inhibit β-cat, FZD7, MMP2, and breast cancer growth via interrupting HIF-1α/VEGF signaling and might be used as a promising antiangiogenic agent for breast cancer treatment.

Assessment of Biochemical Parameters in Sudanese Male Cigarette Smoker in Khartoum State in Omdurman

Background: Hundreds of thousands around the world die from a disease caused by smoking cigarettes. Cigarette smoking (CS) is considered a worldwide major cause of preventable morbidity and mortality. The main clinical consequences of prolonged exposure to CS are chronic respiratory diseases, increased incidence of a variety of cancers, cigarette smoking had a dangerous effect on the essential biochemical mechanisms on the human body. Objective: This study aimed to assess the liver function in Sudanese male cigarettes smoker to identify the influence of cigarettes smoking on the level of their parameters. Material and methods: The study was designed as case control and include 81 samples, which is divided into case (51 samples) and control group (30 samples). The data collected by the use of questionnaire and blood specimens, and the levels of the parameters is measured by A15 automation spectrophotometer. Then the collected data is analyzed by the use of SPSS. Results: The results of the study showed statistically significant increase in total bilirubin and liver enzymes in case group when compared with control group. The mean of plasma total bilirubin, GOT (AST), GPT (ALT), and ALP levels in case group is (0.663, 26.49, 18.82 and 159.27) and in control group is (0.543, 23.00, 14.30 and 146.63) with p. value (0.001, 0.041, 0.004 and 0.000) respectively. And showed statistically insignificant decrease in total protein and albumin in case group when compared with control group, the means of total protein and albumin in case group is (66.47 and 33.73) and in control group is (68.30 and 34.43) with p. value (0.322 and 0.403) respectively. Conclusion: The study concluded that there was significant increase in liver enzymes (GOT, GPT, and ALP) and total bilirubin in cigarette smoking. And significant decrease in total protein and albumin, in cigarette smoking when compared to nonsmoking.

Extender Supplementation with Glutathione (GSH) and Taurine Improves In Vitro Sperm Quality and Antioxidant Status of New Zealand Rabbits during Chilled Storage for up to 72 hours.

This study assessed the influence of supplementing the rabbit semen extender with various concentrations of glutathione (GSH) and taurine at 24, 48, and 72 h postchilling at 5°C. Semen samples were collected from 20 New Zealand bucks, and ejaculates with standard color, motility (>85%), about 0.5 mL volume, and ∼400 × 106/mL concentration were used and diluted with extenders supplemented with 0.5, 1, and 2 mM of GSH and 1, 5, and 10 mM of taurine and chilled at 5°C. Nonsupplemented samples were used as a control. Sperm's progressive motility, acrosome reaction, and extracellular oxidative stress biomarkers such as MDA contents and GPx, SOD, and CAT concentrations and intracellular transcriptomic levels of SOD and CAT genes were assessed. GSH and taurine supplementation improved the sperm's kinetics by reducing cooling-associated stress, which was ascertained by lowering MDA concentration and increasing SOD, CAT, and GPx concentrations (P < 0.05). Increasing the levels of antioxidant enzymes in the extender was due to the increasing mRNA copies of the SOD and CAT genes (P < 0.05). Furthermore, GSH and taurine maintained the fructose levels in the extender and lowered the GPT levels, which implies sperm membrane stability is maintained through GSH and taurine supplementation. GSH and taurine supplementation to the extender had protective influences on the in vitro rabbit semen quality during chilled storage for up to 72 h, which were remarkable with increasing supplementation dose and cooling time at 5°C.

960 EARLY ONSET OF SUBTLE LIVER DAMAGE IN TTR AMYLOIDOSIS PATIENTS TREATED WITH TAFAMIDIS

Abstract Background misfolded transthyretin amyloid fibrils can deposit in the myocardium causing transthyretin amyloid cardiomyopathy (ATTR-CM). Tafamidis has become recently available for the treatment of ATTR-CM, but to date very few data about its possible side effects have been published. Aims to investigate the potential liver toxicity of Tafamidis 61 mg/day in a group of patients affected by wild type ATTR-CM treated in our department. Materials and Methods 14 consecutive patients with wild-type ATTR-CM were treated with Tafamidis accordingly to the classical recommendations. The patients’ liver function was checked through transaminases and gammaGT determination at baseline and for the first six months and their changes analysed with the non-parametric Wilcoxon test for paired samples. Results mean age of the patients included (13 males and a female) was 78 years. Baseline transaminases were all within the normal range (median GOT level 25.0 U/L - IQR 19.0-29.0; median GPT levels 24.0 U/L - IQR 15.0-27.0) and remained stable for the first two months (median GOT at 2 months 25.5 U/L - IQR 19.0-28.0; median GPT at 2 months 22.5 U/L – IQR 16.0-27.0). Median GGT baseline level was 138.0 U/L (IQR 58.0-204.0) and raised to 142.0 U/L (IQR 56.0-270.0) after a two-month therapy (p&amp;lt;0.01). The further follow-up confirmed a similar trend in the first six month of treatment, with a linear and constant increase of gammaGT levels. Conclusions even not clinically relevant, the wild-type ATTR-CM patients included in our analysis showed all a subtle increase of gammaGT values after only two months of Tafamidis treatment, which was confirmed at six months. This observation underlines the potential hepatotoxicity of the drug, thus confirming the recommendations of strict and regular liver function analysis provided by the manufacturer.