AbstractDriven by the availability of food subsidies and landscape transformation, large carnivore populations are increasingly inhabiting the vicinity of humans. To persist in human proximity, while avoiding conflict and mortality, they must make adjustments in their spatial behavior. Understanding such behavioral adaptations can be crucial to formulate conservation strategies. We used GPS location data to study the movement and space use of protected Arabian wolves in the extremely arid Negev Desert (Israel), where natural prey densities are reduced. We examined wolf preference for the proximity of human infrastructure, while testing the hypothesis that wolves would use areas where ungulates were more likely to occur. Wolves showed a strong spatial association with humans, spending a large proportion of their time in proximity of human infrastructure and moving primarily along roads. Wolves also increased their movement during the night. In addition, wolves that had higher availability of human infrastructure in their home ranges showed increased selection for such features, suggesting a positive functional response. Finally, wolves did not show preference for areas of high ungulate occurrence at either spatial scale. These findings, combined with recent diet analyses, indicate that wolves rely on human subsidies, proportioned by the availability of garbage and agricultural produce. Our results demonstrate that wolves fine‐tune their space use to persist in the vicinity of humans. Additional conditions that enable such persistence include effective legal protection and positive local attitudes toward wolves. We conclude that such circumstances can promote spatial coexistence of humans and large carnivores. The strong observed spatial association and evident dietary dependence of wolves on subsidies from a low‐density human population may also reflect the conditions that led to dog domestication.