Gourami Fish Research Articles

Optimizing the gonadal performance of broodstock Helostoma temminckii with addition of enrichment ingredients in feed

The problem in the development of Kissing gourami fish culture was quality seeds that cannot be available continuously. Purpose of this research was to examine the need for additional enrichment materials in feed that can optimize the process of gonad maturation of Kissing gourami fish. Treatment A=Commercial feed added with Vegetable Oil, Glutathione, and Egg White; B=Commercial feed added with Vegetable Oil, Vitamin E 300 mg/Kg, Glutathione, and Egg White; C=Commercial feed added with Vegetable Oil, Vitamin E 500 mg/Kg, Glutathione, and Egg White. and D=Commercial feed added with Vegetable Oil, Vitamin E 700 mg/Kg, Glutathione, and Egg White. The results showed that all treatments with the addition of glutathione and vitamin E fortifications with levels of 300 mg-700 mg gave the effect of increasing IGS, IHS, higher fecundity and larger egg diameter than treatment A. The amount of initial IGS increase in treatment A was 33.49%, treatment B was 172.52%, treatment C was 208.60% and treatment D was 691.20%. While the IHS value at the end of the study decreased in treatment A by 35.30% and treatment C which also experienced a decrease in IHS by 26.23%. While in treatments B and D there was a not too large increase, in the range of 42.56% (B) and 5.66% (D).

EFFECT OF ADDITION OF TEMULAWAK EXTRACT (Curcuma xanthorriza) ON ARTIFICIAL FEED ON GURAMI FISH GROWTH (Osphronemus goramy)

The problem with gouramy cultivation was slow growth due to the short intestines of gouramy so that the absorption of feed takes longer and less. Several previous studies used temulawak in the form of flour, extract, oil which was applied to carp, tilapia, milkfish, catfish. The addition of extract, temulawak flour to the feed gave an increase in growth both in length and weight in the test fish. The purpose of this study was to determine whether or not the effect of temulawak extract on the growth of gouramy fish. This research was conducted for 45 days. This research was conducted by experimental method using a completely randomized design (CRD) with 5 treatments (P) 3 times replication (U), in order to obtain 15 experimental units. Treatment 0 Control (without temulawak extract), Treatment 1 Provision of temulawak extract with a concentration of 14%/kg feed, Treatment P2 Administration of temulawak extract with a concentration of 16%/kg feed, treatment P3 Administration of temulawak extract with a concentration of 18%/kg feed, and treatment P4 administration of temulawak extract with a concentration of 20%/kg feed. The results showed that in the P0 treatment the absolute weight gain was 0.78 grams, in the P1 treatment the absolute weight was 0.58 grams, the P2 treatment was 0.33 grams, the P3 treatment was 0.37 grams, and in the P4 treatment it was obtained The result of absolute weight gain was 0.39 grams. The results showed the absolute length increase ranged from 0.35-0.57 cm. It can be concluded that the addition of temulawak extract to the commercial feed of carp has no significant effect on absolute weight, absolute length, survival rate and feed efficiency level.

Penyuluhan budidaya Magot sebagai pakan alternatif pada budidaya Ikan Gurami di Kecamatan Negara Kabupaten Jembrana Provinsi Bali

Magot is a larva of the insect Hermetia illucens that can convert organic waste into high protein. Magot can be used as one of the alternative feeds of gourami fish that has a protein content equivalent to pellet feed. This is very necessary by cultivators in the Negara District who have problems regarding the increasingly high price of pellets causing high operational costs and Lack of Knowledge of Skills and Attitude of cultivators towards alternative feed that can substitute the pellet feed. The assistance of this group was carried out for approximately 3 months at POKDAKAN Mina Harapan. The purpose of this activity, which is to increase the knowledge of cultivator attitude skills on the application of BSF maggot as an alternative feed for gourami fish. The type of data collected in the form of primary data and secondary data, data collection is done through observation and interview. Data collected in qualitatively denitrifying analysisThe result of this activity is an increase in knowledge and target skills regarding magot culture and changes in the target to positive direction regarding the material provided. The addition of maggot as an alternative feed in dempond activities can be projected into 10 months of maintenance then the profits obtained using maggot BSF as an alternative feed of Rp 6,020,420, while if only using the benefits obtained in 10 months amounted to Rp 3,471,000 with 500 fish scattered.

Improving the Quality of Frozen Fillets of Semi-Dried Gourami Fish (Trichogaster pectoralis) by Using Sorbitol and Citric Acid.

Semi-dried gourami fish (Trichogaster pectoralis) is popularly consumed domestically and exported as a frozen product. This study was conducted to prevent deterioration quality in frozen fish fillets during storage. This research aims to investigate the effects of sorbitol and citric acid at concentrations of 2.5% and 5% (w/w) of frozen gourami fish fillets compared to the fillets soaked in distilled water on physicochemical properties, such as cooking loss, cooking yield, drip loss, pH, TBARS, color, and texture profile analyses (TPA) during storage at −18 ± 2 °C for a period of 0, 20, and 40 days. The fish soaked in sorbitol and citric acid solutions had significantly (p < 0.05) higher protein and fat contents than the control sample. Sorbitol was able to retain moisture in the product; therefore, the drip loss and cooking loss were the lowest, and cooking yield was the highest among other samples (p < 0.05). The addition of 5% (w/v) citric acid in frozen fish fillets can significantly retard the thiobarbituric acid reactive substance (TBARS) (p < 0.05) during storage when compared to fish soaked in sorbitol solution kept for the same period. However, the addition of citric acid resulted in low quality in texture and color of frozen fish fillets. The use of sorbitol was the best alternative in frozen fish fillet product due to reducing the negative effects of freezing quality of the products and generating a cryoprotective effect compared to the fillets soaked in distilled water.

THREE SPOT GOURAMI (Trichogaster trichopterus) FOOD HABITS IN THE KOMERING RIVER'S DOWNSTREAM, SOUTH SUMATERA

One of the downstream areas of the Komering River with potential for capture fisheries is Sungai Dua Village. One of the types of fish caught by fishermen in Sungai Dua is the three spot gourami (Trichogaster trichopterus). The three spot gourami fish is a type of freshwater consumption fish that has high economic value. But the fishes only rely on catches from nature. If the fishing is carried out continuously, without domestication and cultivation, it will cause a decline in the fish population. The research about the biological aspects of red-eye septic fish (Trichogaster trichopterus) which include food habits, relationship length and weight, condition factors and stomach fullness index. This research was conducted from April to June 2017. Sampling was carried out in the downstream waters of the Komering River in Sungai Dua Village, Banyuasin Regency, South Sumatra. Fish samples were analyzed at the Animal Taxonomy Laboratory, Department of Biology, Faculty of Mathematics and Science, Sriwijaya University. The determination of stations in sampling was carried out using multiple sampling methods, namely purposive sampling and quota sampling. The result of this research showed that (1) the Cyanophyta is the main food for the three spot gourami, Diatomae and Desmidiacae as a complementary food, while the additional foods are Rhizopoda, Chlorophyta, Ostracoda, and Entomostraca, (2) The growth pattern of red-eyed fish is negative allometric with a b value of -2 (b &lt;3), the increase in length is faster than the weight gain, (3) The highest condition factor value is found in the size range 6.01-7.00 cm of 23.25 gr / cm and the lowest condition factor value is in the size range 5.01-6.00 cm of 0.62 gr / cm, and (4) The highest hull fullness index value of the red eye sepat fish hull is in the size range 7.01-8.00 cm at 32.86% and the lowest hull fullness index value at the size range 8.01-9.00 cm is 2.05% .

Application of Chitosan From Waste Gurami Fish Scales (Osphronemus goramy) and Clove Powder (Syzygium aromaticum) As Edible Coating on Chicken Meat

Chicken meat is a nutritious food that can increase immunity but has perishable food properties that are easily damaged so it is necessary to add preservatives, namely chitosan as an edible coating to protect the meat from microbial contamination. Gourami (Osphronemus goramy) scales have the potential to be processed as chitosan. Through 3 stages, namely deproteination, demineralization, and deacetylation. The purpose of this study was to determine the effect of adding chitosan with gourami fish scales and cloves as an edible coating on the quality of raw chicken meat at room temperature for 9 h. This study used a factorial completely randomized design (3×3), with factor A: concentration of chitosan solution (0%, 1%, and 2%) and factor B: concentration of clove solution (0%, 1%, and 2%) and repeated 3 times. The results showed that the control treatment without clove and chitosan after 9 h of storage at room temperature had a significant difference in effect on the treated samples. The best treatment was found in the interaction of 2% chitosan (K2) and 2% cloves (C2) with microbial contamination of 2×10 5 CFU/g so that it still meets the standards of the National Standardization Agency (BSN) namely, chicken meat has a maximum requirement of 1×106 CFU/g, which can maintain the quality of meat, both in terms of pH, water content, and acceptability of organoleptic values which are still favored by panelists.

Isolation and selection of bacteria inhibiting Streptococcus agalactiae causing dark-body disease on snakeskin gourami fish (Trichogaster pectoralis)

Streptococcus agalactiae is one of the microbial pathogens causing the dark-body disease on snakeskin gourami fish (Trichogaster pectoralis) that affects the growth and quality of fish. This research aimed to isolate and select bacteria inhibiting S. agalactiae which are able to use for controling pathogenic bacteria instead of antibiotics. Fourteen bacteria strains were isolated and screened from healthy fishes, sediment and water samples at fish ponds in Dong Thap province. Among these strains, L7 strain showed the highest inhibition ability with the clear zone diameter was 9,3 mm. The results of the 16S rRNA sequence analysis indicated that the L7 strain belonged to Bacillus subtilis. The experiment to evaluate the inhibition capacity and fish disease control of selected B. subtilis in experimental conditions was conducted by challenging fish with S. agalactiae. Fishes in the control treatment was infected with S. agalactiae at 106 CFU/mL had survival rate 41,7%. The experimental treatments NT1, NT2, NT3 which were treated with B. subtilis at concentrations of 105 CFU/mL, 106 CFU/mL, and 107 CFU/mL gave higher survival rates compared with the non-treated control, with the rates of 60%, 76,7%, and 81,7%, respectively. These results revealed that the isolated B. subtilis is potential used in control dark-body disease on snakeskin gourami fish.

Effect of Feed Combination of Avocado (Persea americana M.) and Pumpkin Seed (Cucurbita moschata Duch.) on Villi Height and Goblet Cell Number of Goblet Fish (Osphronemus gourami Lac.)

In Indonesia, there are several types of freshwater fish that the community has cultivated. Besides having high nutritional value, Freshwater fish also have high economic value for trading, such as gourami. One of the obstacles in the cultivation of freshwater fish is the price of feed. This research is oriented towards alternative feeds to reduce production costs by utilizing avocado seeds and pumpkin seeds. This study aimed to determine the effect of feeding a combination of avocado seeds and pumpkin seeds on the histopathology of gourami. This study used a non-factorial completely randomized design (CRD) method with four treatments and six replications P0, P1, P2, and P3. P0 (Control feed/pellet), P1 (Feed 40 grams of avocado seeds + 40 grams of pumpkin seeds + 20 grams of mixed ingredients), P2 (Feed of 20 grams of avocado seeds + 60 grams of pumpkin seeds + 20 grams of mixed ingredients) and P3 ( Feed 60 grams of avocado seeds + 20 grams of pumpkin seeds + 20 grams of mixed ingredients). Data were analyzed using ANOVA and Advanced Test with a significant level of 5%. The results showed that the P2 treatment could increase the villi height and the number of Goblet cells in the intestines of gourami fish. It can be concluded that the combination of avocado seeds and pumpkin seeds has a significant effect on the histopathological picture of the digestive organs of gourami

Nutrient loading and farm characteristics of giant gourami fish aquaculture systems in Lake Maninjau, Indonesia: basic knowledge of production performance

Background Aquaculture systems for giant gourami, Osphronemus goramy Lacepède (1801), have significantly improved fish production yields and food security in Indonesia. However, these systems also cause serious problems in terms of eutrophication in waterbodies. This study analysed the nutrient loading and farm characteristics of giant gourami in floating cages in Lake Maninjau. Method A total of 20 floating cages were used to record these nutrients in feed supply, female and male juvenile fish, dead fish and harvested fish to estimate nutrient loading. Data on the harvested fish, production cycle, stock number and cage capacity were used to estimate the stocking density, feeding rate, feed efficiency, and net fish yield, and the relationship between feed supply and nutrient loading and farm characteristics was analysed by least squares regression methods. Results A total of 20 floating cages released nutrients into waterbodies at an average rate of 236.27±60.44 kg/cycle for C, 84.52±20.86 kg/cycle for N and 8.70±3.63 kg/cycle for P. On average, fish production for each floating cage (±SD) was 1226±282 kg wet weight/cycle, and the net fish yield was 12.63±2.82 kg/m3/cycle. Survival rates ranged from 86.33 to 95.27%/cycle. The production cycles varied from 160 to 175 days with feed conversion ratios between 1.60 and 1.75, feed conversion efficiencies were between 0.58 and 0.63. The production parameters that had strong relationships with the net fish yield were feed supply (r2=0.960), stocking rates (r2=0.924) and feeding rates (r2=0.961). In contrast, the length of the production cycle was not strongly related to the net fish yield (r2=0.187). Conclusion Nutrient loading from the supplied feed was greater than that from the harvested fish, juvenile fish and dead fish. Increasing the net fish yield in floating cages was better predicted by the stocking densities and feeding levels than by the other factors.

Infectious spleen and kidney necrosis virus-associated large-scale mortality in farmed giant gourami, Osphronemus goramy, in India.

Megalocytivirus cause diseases that have serious economic impacts on aquaculture, mainly in East and South-East Asia. Five primary genotypes are known: infectious spleen and kidney necrosis virus (ISKNV), red sea bream iridovirus (RSIV), turbot reddish body iridovirus (TRBIV), threespine stickleback iridovirus (TSIV) and scale drop disease virus (SDDV). ISKNV-mediated infectious spleen and kidney necrosis disease (ISKND) is a major viral disease in both freshwater and marine fish species. In this study, we report the isolation of ISKNV from diseased giant gourami, Osphronemus goramy, in India. Transmission electron microscopy of ultrathin sections of kidney and spleen revealed the presence of numerous polygonal naked viral particles having an outer nucleocapsid layer within the cytoplasm of enlarged cells (115-125nm). Molecular and phylogenetic analyses confirmed the presence of ISKNV and the major capsid protein (MCP) (1,362bp) gene in the infected fish had a high similarity to the other ISKNV-I isolates. Moreover, ISKNV was propagated in the Astronotus ocellatus fin (AOF) cell line and further confirmed genotypically. A high mortality rate (60%) was observed in gourami fish injected with ISKNV-positive tissue homogenate through challenge studies. Considering the lethal nature of ISKNV, the present study spotlights the implementation of stringent biosecurity practices for the proper control of the disease in the country.

Nutrient loading and farm characteristics of giant gourami fish aquaculture systems in Lake Maninjau, Indonesia: basic knowledge of production performance

Background Aquaculture systems for giant gourami, Osphronemus goramy Lacepède (1801), have significantly improved fish production yields and food security in Indonesia. However, these systems also cause serious problems in terms of eutrophication in waterbodies. This study analysed the nutrient loading and farm characteristics of giant gourami in floating cages in Lake Maninjau. Method A total of 20 floating cages were used to record these nutrients in feed supply, female and male juvenile fish, dead fish and harvested fish to estimate nutrient loading. Data on the harvested fish, production cycle, stock number and cage capacity were used to estimate the stocking density, feeding rate, feed efficiency, and net fish yield, and the relationship between feed supply and nutrient loading and farm characteristics was analysed by least squares regression methods. Results A total of 20 floating cages released nutrients into waterbodies at an average rate of 236.27±60.44 kg/cycle for C, 84.52±20.86 kg/cycle for N and 8.70±3.63 kg/cycle for P. On average, fish production for each floating cage (±SD) was 1226±282 kg wet weight/cycle, and the net fish yield was 12.63±2.82 kg/m 3/cycle. Survival rates ranged from 86.33 to 95.27%/cycle. The production cycles varied from 160 to 175 days with feed conversion ratios between 1.60 and 1.75, feed conversion efficiencies were between 0.58 and 0.63. The production parameters that had strong relationships with the net fish yield were feed supply ( r 2=0.960), stocking rates ( r 2=0.924) and feeding rates ( r 2=0.961). In contrast, the length of the production cycle was not strongly related to the net fish yield ( r 2=0.187). Conclusion Nutrient loading from the supplied feed was greater than that from the harvested fish, juvenile fish and dead fish. Increasing the net fish yield in floating cages was better predicted by the stocking densities and feeding levels than by the other factors.

KAJIAN PEMBERIAN PAKAN BERBAHAN BAKU LOKAL DENGAN KANDUNGAN PROTEIN YANG BERBEDA TERHADAP PERTUMBUHAN IKAN GURAME (Osphronemus goramy Lac.)

Gourami fish (Osphronemus goramy Lac.) is a freshwater fish commodity that has high economic value because its very popular with all people. But the feed commonly used by gourami fish cultivators is factory-made feed, which is a commercial feed that usually costs quite a lot of money due to the material for making commercial feed, some of which still import from abroad. Therefore it is necessary to find a solution to reduce the cost of cultivation production by replacing commercial feed with homemade feed made from local raw materials. The purpose of this study was to examine the use of local raw materials formulated to make feed suitable for the growth of gourami fish (Osphronemus goramy Lac.) And to analyze the production costs of goramy feed made from local raw materials. This study consisted of 4 treatments and 3 replications using the completely randomized design (CRD) method. The treatments consisted of treatment A (30% protein), treatment B (33% protein), treatment C (36% protein), and treatment D (commercial). The results of this study indicate that the treatment of feed A, B, and C produced the same growth as commercial feed, and treatment of feed A resulted in the lowest feed production costs compared to other treatments. Key word: feed, growth, local raw materials, Oshphronemus goramy

Assessing the susceptibility of the selected gourami (Osphronemus goramy) to Aeromonas hydrophila

Abstract. Febrianti R, Khasani I, Rosada KK. 2021. Assessing the susceptibility of the selected gourami (Osphronemus goramy) to Aeromonas hydrophila. Nusantara Bioscience 13: 111-120. A breeding program to improve the growth performance of the gourami fish was carried out through selection methods that produced faster growth gourami (selected population). The purpose of this study was to determine the susceptibility of the selected gourami to Aeromonas hydrophila infection based on tolerance limits (LD50) and investigated clinical signs post-injection of the pathogenic bacteria. The challenge test by intramuscular injection of A. hydrophila was done to the fingerling fish (15-20 g) for 14 days post-infection. The population of the tested fish was obtained from six families, selected gourami (SP), and non-selected control (CP) groups. Phosphate buffered saline (PBS) as control and several doses of the pathogen, 102, 104, 105, 106, 107, and 108 CFU/mL of A. hydrophila, were injected into the fish. Fish mortality and clinical signs were observed daily. The fish mortality was confirmed by isolating bacteria in the fish which showed clinical signs, followed by biochemical characterization of the isolated bacteria using API 20E and PCR. The LD50 of A. hydrophila to the selected population (9.70 × 105 CFU/mL) was higher than that of the control (6.50 × 104 CFU/mL). On the final day of the test, the accumulation mortality of CP (63.33±5.77%) higher than that in SP (33.33±5.77%). Based on the output test statistics, it was known that there was a significant difference between the mortality and clinical signs of SP and CP. The data suggested that the selected gourami were more resistant to A. hydrophila infection than that of the control. The A. hydrophila infection caused most of the major clinical signs, including mass mortality of the fish. The biochemical and PCR test ensure that fish mortality was caused by A. hydrophila infection.

Nutrient loading and farm characteristics of giant gourami fish aquaculture systems in Lake Maninjau, Indonesia: basic knowledge of production performance.

Background Aquaculture systems for giant gourami, Osphronemus goramy Lacepède (1801), have significantly improved fish production yields and food security in Indonesia. However, these systems also cause serious problems in terms of eutrophication in waterbodies. This study analysed the nutrient loading and farm characteristics of giant gourami in floating cages in Lake Maninjau. Method A total of 20 floating cages were used to record these nutrients in feed supply, female and male juvenile fish, dead fish and harvested fish to estimate nutrient loading. Data on the harvested fish, production cycle, stock number and cage capacity were used to estimate the stocking density, feeding rate, feed efficiency, and net fish yield, and the relationship between feed supply and nutrient loading and farm characteristics was analysed by least squares regression methods. Results A total of 20 floating cages released nutrients into waterbodies at an average rate of 236.27±60.44 kg/cycle for C, 84.52±20.86 kg/cycle for N and 8.70±3.63 kg/cycle for P. On average, fish production for each floating cage (±SD) was 1226±282 kg wet weight/cycle, and the net fish yield was 12.63±2.82 kg/m 3/cycle. Survival rates ranged from 86.33 to 95.27%/cycle. The production cycles varied from 160 to 175 days with feed conversion ratios between 1.60 and 1.75, feed conversion efficiencies were between 0.58 and 0.63. The production parameters that had strong relationships with the net fish yield were feed supply ( r2=0.960), stocking rates ( r2=0.924) and feeding rates ( r2=0.961). In contrast, the length of the production cycle was not strongly related to the net fish yield ( r2=0.187). Conclusion Nutrient loading from the supplied feed was greater than that from the harvested fish, juvenile fish and dead fish. Increasing the net fish yield in floating cages was better predicted by the stocking densities and feeding levels than by the other factors.

Detection Moleculer Of Putative 18S rRNA Gen Protozoa Trichodina sp. Infected Larvae Gurami (Osphronemus gouramy L) in Balai Benih Ikan Kutasari Purbalingga Central Java

Trichodina spp. are ectoparasitic pathogens of ciliata group that commonly infect both freshwater and marine fish, including gouramy fish. As a result of infection of Trichodina spp. this will lead to inhibition of fish growth and decreased fish production, resulting in low fish selling value. The rate of occurrence of Trichodina spp. that infects gurami can reach 100%. Research has been conducted to determine which one Trichodina spp. Protozoa that infects the gouramy seeds of BBI (Fish Seed Center) Kutasari Purbalingga following detection of 18S RNA gene. Gene detection method used in this research is Polymerase Chain Reaction (PCR) is a technique of DNA synthesis and amplification in vitro. This research is done following these methodes: (1) sampling of Gurami fish with purposive sampling which obtained from BBI Kutasari Purbalingga, (2) isolation of Trichodina spp., (3). Preparation of Trichodina spp. sample and its identification, and (4). Molecular character obervation following detection of 18S rRNA gene. This study obtained 10% percentage of detection of 18S rRNA genes of the species of Trichodina paraheterodentata that infect on the gouramy fish of Purbalingga. The percentage rate of detection of these genes is low when compared with the results of the detection of 18S rRNA Trichodina paraheterodentata gene that infects gouramy fish in Banjarnegara.

Identification and Characterization of EvpQ, a Novel T6SS Effector Encoded on a Mobile Genetic Element in Edwardsiella piscicida.

In this study, a hypothetical protein (ORF02740) secreted by Edwardsiella piscicida was identified. We renamed the ORF02740 protein as EvpQ, which is encoded by a mobile genetic element (MGE) in E. piscicida genome. The evpQ gene is spaced by 513 genes from type VI secretion system (T6SS) gene cluster. Low GC content, three tRNA, and three transposase genes nearby evpQ define this MGE that evpQ localizes as a genomic island. Sequence analysis reveals that EvpQ shares a conserved domain of C70 family cysteine protease and shares 23.91% identity with T3SS effector AvrRpt2 of phytopathogenic Erwinia amylovora. Instead, EvpQ of E. piscicida is proved to be secreted at a T6SS-dependent manner, and it can be translocated into host cells. EvpQ is thereof a novel T6SS effector. Significantly decreased competitive index of ΔevpQ strain in blue gourami fish (0.53 ± 0.27 in head kidney and 0.44 ± 0.19 in spleen) indicates that EvpQ contributes to the pathogenesis of E. piscicida. At 8-, 18-, and 24-h post-subculture into DMEM, the transcription of evpQ was found to be negatively regulated by Fur and positively regulated by EsrC, and the steady-state protein levels of EvpQ are negatively controlled by RpoS. Our study lays a foundation for further understanding the pathogenic role of T6SS in edwardsiellosis.

The influence of garlic (Allium sativum) and turmeric (Curcuma longa) extract as attractant in commercial feed on feeding rate and fat retention in gouramy (Osphronemus gouramy)

Gouramy fish known as fish that has slow growth but the demand is always increasing every year. Feed improvement can be developed by adding attractants to the feed. Attractant will produce an odor that will attract fish to eat. The sulfur components that dominates garlic is believed to be attractant, while in turmeric the terpenoid can add aroma to the feed. Furthermore, the sulfur components in garlic and curcumin in turmeric can increases fat metabolism. Feed energy can be seen from the calculation of the amount of fat consumption and fat retention. The method used in this study is an experimental method with 5 treatments; P0= control, P1; 1% garlic extract addition, P2; 1.5% garlic extract addition, P3= 1% turmerix extract addition, and P4= 1.5% turmeric extract addition. The results showed that the addition of 1-1.5% turmeric extract was able to reduce fat consumption, while the addition of 1-1.5% garlic extract was able to reduce the fat retention of gouramy. It can be concluded that giving 1-1.5% garlic extract does not affect the fat consumption of gouramy but reduces fat retention, while the addition of 1-1.5% turmeric can reduce fat consumption and doesnt affect fat retention of the fish.

The effect of garlic (Allium sativum) and turmeric (Curcuma longa) extract addition in commercial feed on feeding rate, feed efficiency and feed conversion ratio of gouramy fish (Osphronemus gouramy)

Gouramy is known to it’s low appetite and slow growth. Attractants are ingredients that are mixed in small amounts of feed to increase feed intake, growth, and fish consumption of feed. Garlic contains at least 33 sulfur components that give a distinctive odor and various medicinal effects. Turmeric contains essential oils composed of monoterpenes and is an effective odor stimulating precursor compound used as an attractant. This study aims to determine the effect of giving turmeric and garlic extracts at different doses on feed consumption, feed efficiency and feed conversion ratio in gouramy. This study used an experimental method with 5 treatments; P0= control, P1=1% garlic extract addition, P2= 1.5% garlic extract addition, P3= 1% turmeric extract addition and P4= 1.5% turmeric extract additions with 4 replications using 10 fish in each.. The results showed that the highest feed consumption value was in the treatment of P1and P2, the lowest conversion ratio and the highest value of feed efficiency was found in the treatment of P2 and P3. So it can be concluded that the addition of 1.5% garlic extract or 1% turmeric extract is effective as an attractant and effective for increasing the growth of gouramy.

Development of a Participatory Health Care Model for Farmers and Stakeholders Involved in Snakeskin Gourami Fish Farming in Bang Bo District, Samutprakarn Provinces, Thailand

The present study was based on a participatory action research (PAR) design with the objectives of developing a participatory health care model for farmers and stakeholders involved in snakeskin gourami fish farming in Bang Bo District. The study was conducted from May 1, 2017 - April 30, 2018. The sample was composed of 85 people who were fish farmers, fish pond owners, and fish farming laborers involved in the processing and distribution of snakeskin gourami, 35 VHVs (village health volunteers), 1 registered nurse, and 1 administrator of a Tambon Health Promoting Hospital (THPH) for a total of 122 subjects. The PAR process involved 3 stages: Stage 1- Situation Analysis, Stage 2 - Participatory Action Research Process, and Stage 3 - Outcome Evaluation. The findings revealed 2 types of health problems: 1) work-related problems: incorrect work behaviors such as lifting heavy objects 1 - 5 times per day, repetitive work postures/positions causing aching bones and muscles and working fast to obtain large amounts and finish in time until work-related accidents occur such as knife cuts, pricked fingers from fish fins and slips/fallsand 2) Risks and illnesses caused by chronic disease. The synthesis of the participatory health care model disclosed that the health care activities covered 4 dimensions, encompassing health promotion, disease prevention, treatment, and recovery. The sample had a high level of satisfaction in the organization of the project at a mean of 4.40 of 5 points.

Expression of ALP and TGF-β in Osteoblast Cell Cultures after Administering Collagen Peptide Derived from Gouramy (Osphronemus goramy) Fish Scales

Introduction: Collagen peptide extracted from gouramy fish scale has noncytotoxic effects and good viability on osteoblast cells. Various characteristic tests also show that it could form as scaffold with the potential to be a biomaterial graft used in tissue engineering. Studies in this field are vital considering the fact that graft is highly essential for the development and success of regeneration therapy. The aim of the study was to examine the expression of alkaline phosphatase (ALP) and transforming growth factor-β (TGF-β) in osteoblast cell cultures after administering collagen peptide derived from gouramy fish scale. Material and Methods: Osteoblast cells were put into 60 well plates divided into two groups. The first group was to analyze the expression of ALP, while the second group was for TGF-β. Then, each of the group was divided into five other groups consisting of cell control and culture of osteoblast cell having collagen peptide derived from gouramy fish scale with four different concentrations. The collagen peptide was extracted through enzymatic method. Then, the immunocytochemistry assay was used to detect the expression of ALP and TGF-β in the osteoblast cell cultures derived from calvaria mice after administering collagen peptide from the fish scale. The statistical methods used include Multivariate Analysisof Variance (MANOVA) analysis with a significance value of 0.000 (P < 0.05) and Tukey Honest Significance Test (HSD). Results: The statistical results showed that the collagen peptide derived from gouramy fish scales with various concentrations produced significantly different expressions of ALP and TGF-β. Conclusion: The expression of ALP and TGF-β in osteoblast cell cultures increased after administering the collagen peptide derived from the gouramy fish scales.