Previous research has documented several reliable differences between men and women in terms of mate preferences regarding age, physical appearance, financial prospects, and more. However, most of the research has been on heterosexual populations. The current study attempted to further explore those differences in non-heterosexual populations. The project was part replication regarding heterosexual populations and part exploratory regarding non-heterosexual populations. The sample contained 3298 participants, including 1863 males (1675 gynephiles, 56 androphiles, 132 bisexuals) and 1435 females (1037 androphiles, 33 gynephiles, 365 bisexuals). Participants responded to questions about mate preferences in terms of good financial prospects, good looks, chastity, ambition/industriousness, youth/age, uncommitted sex, visual sexual stimuli, status, physical attractiveness, jealousy, and interest in short- versus long-term mating. Results replicated typical sex differences between heterosexual men and women in all measures we analyzed. We also found several instances when bisexual respondents were more different from heterosexual respondents than homosexual respondents (specifically regarding interest in uncommitted sex, the importance of chastity, and interest in short-term mating). Despite limitations in data collection, the results demonstrate that homosexual and bisexual individuals do not always form a heterogenous group.