Gondii IgG Research Articles

Evaluation of T. gondii, rubella, and cytomegalovirus seroprevalences among female Syrian refugees in Sanliurfa, Turkiye.

Since the Syrian Civil War began in 2011, the official number of refugees under temporary protection in Turkiye is reported to be 3,522,036 in 2023. Most of the Syrians living outside the refugee camps have worse conditions in terms of access to healthcare centers and social opportunities, compared to those living in camps. The Sanliurfa province hosts the third highest number of Syrians (370,291) in Turkiye. There are no data about the seroprevalence of Toxoplasma gondii (T. gondii), rubella (rub), or cytomegalovirus (CMV) among Syrian refugees in Sanliurfa. We aimed to investigate the seroprevalence of T. gondii, rub, and CMV infections among female Syrian refugees of reproductive age (15-49 years) living in Sanliurfa province. A cross-sectional study was conducted in different districts of Sanliurfa. A total of 460 households were selected using the probability sampling method. One married female Syrian refugee aged between 15 and 49 years, was chosen in each household, leading to a sample size of 410 female Syrian refugees. The seropositivity of T. gondii, CMV, and rub IgM and IgG in blood samples were analyzed using enzyme immunoassays (Abbott Architect, Illinois, USA). The seropositivity rates of T. gondii, CMV, and rubella IgM and IgG were 4.4% and 59.8%; 3.9%; and 99%; and 1.9%, and 99.5%, respectively. A screening program should be implemented for T. gondii, CMV, and rub infections for Syrian refugees. Seronegative women should be vaccinated against rub and educated about the transmission and preventive routes of toxoplasmosis and CMV infection.

The Prevalence of Toxoplasma Gondii Infection in Duhok, Kurdistan Region, Iraq

Toxoplasmosis is a parasitic infection, with widespread gestational and congenital transmission rates globally, consequently, it is regarded as a public health concern and an overlooked medical condition. Human infection occurs through various routes, including the ingestion of oocysts found in cat feces, consumption of raw or undercooked meat containing the parasite, and vertical transmission from an infected pregnant mother to her fetus. Objective. The aim of this study is to determine the prevalence of Toxoplasma gondii infection among individuals in various age groups and genders in Duhok City. Methods. In this research, 2251 individuals of various ages and genders visited the central laboratory in Duhok Сity, Kurdistan Region, Iraq, and peripheral blood samples were taken from individuals to identify specific anti-Toxoplasma gondii IgM and IgG antibodies using serological test (ELISA test). Results. Based on the findings of this study, it was observed that the prevalence of toxoplasmosis among females with infections detected in both IgM and IgG was 0.3 % and 20.6 %, respectively, and in males, the rate of infection with IgG was found to be 0.3 %, while no infections were detected with IgM. Also, among female individuals, the study recorded a 0.3 % infection rate for toxoplasmosis, which encompassed both IgM and IgG cases. Conclusions. Based on this study, it can be concluded that Toxoplasma infection is more common in females than in males, chronic toxoplasmosis appears to be more prevalent than acute infection with the highest rate of acute infections was found in the age group of 21–40 years.

COMPARISON SEROPREVALENCE OF TOXOPLASMA GONDII, RUBELLA, AND CYTOMEGALOVIRUS IN MUŞ PROVINCE BEFORE AND DURING THE COVID-19 PANDEMİC

The aim of this study is to determine the seroprevalence of Toxoplasma gondii (T. gondii), rubella and cytomegalovirus (CMV) and the primary infection rates of these agents with avidity test in pregnant women in Muş province. Additionally, the effect of prevention and protection measures implemented during the Covid-19 pandemic on the prevalence of T. gondii, rubella and CMV in pregnant women was also investigated. Between 2018 and 2021, IgG and IgM antibodies in the serum samples of pregnant women who applied to the Department of Gynecology and Obstetrics polyclinic of Muş State Hospital were examined by enzyme-linked immunosorbent assay (ELISA) and chemiluminescence immunoassay (CLIA). High and low avidity assays were examined by enzyme linked fluorescence assay (ELFA). Low seroprevalence of T. gondii IgG antibody (28%) and the detection of low avidity detection in 38 of 102 pregnant women indicate that this parasite may be a risk for intrauterine life. Rubella IgG (89%) and CMV IgG (99%) antibodies were found to carry a low risk due to their high seroprevalence and avidity. It was determined that Covid-19 prevention and protection measures caused a significant decrease in the prevalence of rubella and CMV, but had no effect on the prevalence of T.gondii.

Serological and molecular survey of Toxoplasma gondii infection and associated risk factors in urban cats in Kunming, Southwest China.

Toxoplasma gondii (T. gondii) is a worldwide zoonotic parasite that can infect almost warm-blood animals, including humans, which seriously affect the health of host. Cats are known to be the only definitive host of T. gondii and continuously excrete highly infectious oocysts. This parasite carried by the companion animals leads to a great public health risk. However, there is little information on epidemiology of T. gondii in urban cats in Kunming, Southwest China. In the present study, a total of 231 serum and fecal samples were collected in Kunming aera, and then seroprevalence of T. gondii IgG antibodies in serum and molecular investigation in feces were analyzed to elucidate T. gondii infection in urban cats. The results revealed that 168 of 231 cats (72.7%) were positive for T. gondii antibodies, and 1 of 74 cat feces (1.4%) also showed a positive PCR for T. gondii DNA. The positive fecal sample was sequenced and then phylogenetically analyzed, and the isolate of T. gondii in the present study was closely related to T. gondii strain CN. In addition, the food, water and age of cats were identified as the risk factor for seropositivity. Overall, our findings indicate the widespread occurrence of T. gondii infection in urban cats in Kunming, Southwest China and identify food, water and age are the risk factors associated with T. gondii infection, which can provide effective information for developing strategies to prevent and control this zoonosis.

In silico study to explore the mechanism of Toxoplasma-induced inflammation and target therapy based on sero and salivary Toxoplasma

We aimed to assess salivary and seroprevalence of Toxoplasma immunoglobulins in risky populations and evaluate drug docking targeting TgERP. A cross-sectional study was conducted in Alexandria University hospitals’ outpatient clinics. 192 participants were enrolled from September 2022 to November 2023. Anti-Toxoplasma IgG and IgM were determined in serum and saliva by ELISA. An in-Silico study examined TgERP’s protein–protein interactions (PPIs) with pro-inflammatory cytokine receptors, anti-inflammatory cytokine, cell cycle progression regulatory proteins, a proliferation marker, and nuclear envelope integrity-related protein Lamin B1. Our findings revealed that anti-T. gondii IgG were detected in serum (66.1%) and saliva (54.7%), with 2.1% of both samples were positive for IgM. Salivary IgG had 75.59% sensitivity, 86.15% specificity, 91.40% PPV, 64.40% NPP, 79.17% accuracy and fair agreement with serum IgG. On the other hand, the sensitivity, specificity, PPV, NPV, and accuracy in detecting salivary IgM were 75.0%, 99.47%, 75.0%, 99.47%, and 98.96%. AUC 0.859 indicates good discriminatory power. Examined synthetic drugs and natural products can target specific amino acids residues of TgERP that lie at the same binding interface with LB1 and Ki67, subsequently, hindering their interaction. Hence, salivary samples can be a promising diagnostic approach. The studied drugs can counteract the pro-inflammatory action of TgERP.

Serum signature of antibodies to Toxoplasma gondii, rubella virus, and cytomegalovirus in females with bipolar disorder: A cross-sectional study

Background and aimImmunity alterations have been observed in bipolar disorder (BD). However, whether serum positivity of antibodies to Toxoplasma gondii (T gondii), rubella, and cytomegalovirus (CMV) shared clinical relevance with BD, remains controversial. This study aimed to investigate this association. MethodsAntibody seropositivity of IgM and IgG to T gondii, rubella virus, and CMV of females with BD and controls was extracted based on medical records from January 2018 to January 2023. Family history, type of BD, onset age, and psychotic symptom history were also collected. Results585 individuals with BD and 800 healthy controls were involved. Individuals with BD revealed a lower positive rate of T gondii IgG in the 10–20 aged group (OR = 0.10), and a higher positive rate of rubella IgG in the 10–20 (OR = 5.44) and 20–30 aged group (OR = 3.15). BD with family history preferred a higher positive rate of T gondii IgG (OR = 24.00). Type-I BD owned a decreased positive rate of rubella IgG (OR = 0.37) and an elevated positive rate of CMV IgG (OR = 2.12) compared to type-II BD, while BD with early onset showed contrast results compared to BD without early onset (Rubella IgG, OR = 2.54; CMV IgG, OR = 0.26). BD with psychotic symptom history displayed a lower positive rate of rubella IgG (OR = 0.50). LimitationsAbsence of male evidence and control of socioeconomic status and environmental exposure. ConclusionsDifferential antibody seropositive rates of T gondii, rubella, and cytomegalovirus in BD were observed.

Correlation between toxoplasmosis and schizophrenia in Egyptian patients and its impact on dopamine serum levels

Toxoplasma gondii, a parasite infecting around one-third of the global population, has been linked to neurological disorders like schizophrenia. Abnormal dopamine levels are linked to the pathophysiology of schizophrenia, but their association remains unclear. This study aimed to investigate the relationship between T. gondii seroprevalence and dopamine serum levels in schizophrenic patients in Egypt. This case-control study included 93 patients diagnosed with schizophrenia and 93 individuals as controls. T. gondii seroprevalence was determined using an enzyme-linked immunosorbent assay (ELISA). Dopamine serum levels were measured using ELISA. Sociodemographic and clinical characteristics were also collected. The study found a higher prevalence of T. gondii IgG antibodies in patients with schizophrenia (68 %) compared to controls (46.2 %). Contact with cats, sausage consumption, and undercooked meat were identified as possible risk factors associated with T. gondii infection. The mean level of serum dopamine was significantly (P < 0.001) higher in patients with schizophrenia (115.3 Pg/ml ±31.8) compared to the control group (75.02 Pg/ml ±26.5). The study found that schizophrenic patients with T. gondii seropositivity had significantly higher dopamine serum levels (mean=145.2 ± 32.1 pg/ml) than those without T. gondii seropositivity (mean=122.5 ± 29.7 pg/ml) (p = 0.001). Logistic regression analysis revealed that T. gondii seropositivity was a significant predictor of increased dopamine serum levels in schizophrenic patients (odds ratio=3.4, 95 % confidence interval=1.8–6.4, p < 0.001). The study suggests that T. gondii seroprevalence may increase dopamine serum levels in Egyptian schizophrenic patients, potentially contributing to dopamine dysregulation in schizophrenia, but further research is needed to confirm these findings and investigate the underlying mechanisms.

Seroprevalence of Fasciola sp. and Toxoplasma gondii Infections in Rural and Urban Inhabitants of Jolfa County, Northwest Iran.

Fascioliasis and toxoplasmosis are the two important zoonotic diseases that are endemic in Iran and share some common transmission routes. The present study is aimed at determining the seroprevalence of human fascioliasis and toxoplasmosis in rural and urban areas of Jolfa County, Northwest Iran. In a cross-sectional study, 600 human sera were collected randomly from humans living in Jolfa County including three cities and 13 villages from 2017 to 2018. Anti-Toxoplasma IgG and anti-Fasciola sp. IgG tests have been performed using the enzyme-linked immunosorbent assay. Four (0.7%) out of 600 human sera showed positive levels of anti-Fasciola IgG. Three out of four seropositive humans were from an urban area, and one (25%) was from rural inhabitants. Considering T. gondii infection, 45% of studied human sera were seropositive for anti-T. gondii IgG. In conclusion, this is the first study reporting Fasciola seropositivity in the area. Based on the findings, human fascioliasis is present in the studied area, Northwest Iran, granted in low prevalence. Considering T. gondii seropositivity, the prevalence is high, yet close to the reports from other regions in the province.

TOXOPLASMOSIS: SEROPREVALENCE IN WOMEN OF REPRODUCTIVE AGE AND APPROACH IN PREGNANCY

Objective: Toxoplasmosis is a parasitosis that is mostly asymptomatic or with mild symptoms. It can lead to severe clinical conditions in immunosuppressed patients who had infections that mostly develop through reactivation, or in congenital infections that develop in fetuses infected as a result of vertical transmission. Since it is possible to prevent congenital toxoplasmosis with appropriate follow-up and treatment, our study aimed to determine the susceptible population and seroprevalence of our region and to evaluate the course of infections in this group. Material and Method: Patients of childbearing age who were evaluated in the last five years in our tertiary hospital were included in our study, and Anti Toxoplasma gondii IgM and IgG results in these patients were evaluated. The course of pregnant women who were thought to have primary infection, the follow-up of these patients and the treatments applied were evaluated. In addition, the follow-up of non-pregnant patients examined were also evaluated. Results: The seropositivity rate was found to be 30.7% (n=1703) in 5545 women included in the study, and the seropositivity rate was 31% (n=1399) in 4503 pregnant women. After detailed evaluations, of 16 pregnant women who were thought to have primary infection during pregnancy and were followed up in our hospital; one underwent medical abortion at the request of the family, and only four used the recommended treatment until the end of pregnancy, and congenital toxoplasmosis was not detected in any newborn. Since the many of pregnant women with possible primary infection (n=28) were not referred to the infectious diseases outpatient clinic, this group was not evaluated in detail and could not be monitored. Of the patients included in the study, 26 (0.4%) were examined to investigate the etiology of fever, 37 (0.6%) were examined to investigate the etiology of lymphadenopathy (LAP), and 29 (0.5%) were examined to investigate the cause of elevated liver function tests. Among the patients monitored with LAP, four (10.8%) were diagnosed with acute toxoplasmosis. Conclusion: As a result, it is very important to determine the seroprevalence of toxoplasmosis, to detect possible primary infections, especially in pregnant women, and to prevent congenital toxoplasmosis by clinicians to guide patients correctly so that the follow-up and treatment of this group of patients is not interrupted.

Seroprevalence and Associated Risk Factors of Toxoplasma gondii in Patients Diagnosed with Schizophrenia: A Case-Control Cross Sectional Study.

The protozoan parasite, Toxoplasma gondii, has been linked to several psychiatric disorders, including schizophrenia. The aim of this study was to assess the prevalence of T. gondii IgG antibodies and risk factors associated with seroprevalence in patients diagnosed with schizophrenia. This seroepidemiological study assessed 196 participants, divided into two groups. The study group consisted of 98 schizophrenic patients and was matched with 98 healthy blood donors. A questionnaire was used to gather information regarding potential risk factors associated with T. gondii seroprevalence. Results revealed a higher seroprevalence of T. gondii IgG antibodies in schizophrenic patients (69.39%, 68/98) when compared to healthy controls (51.02%, 50/98) (OR: 2.18; 95% CI: 1.21-3.9; p = 0.01). Patients with schizophrenia who consumed raw or undercooked meat (80.65%, 25/31) (OR: 3.75; 95% CI: 1.25-11.21, p = 0.02) and those with a lower educational level (77.59%, 45/58) (OR: 3.5; 95% CI: 1.59-7.54, p = 0.002) presented increased T. gondii seropositivity rates versus their control counterparts. Our findings indicate a high T. gondii IgG seroprevalence in patients diagnosed with schizophrenia compared to healthy blood donors. Factors associated with T. gondii seroprevalence were consumption of raw or uncooked meat and a lower educational attainment. This study provided the first data regarding the potential risk factors for toxoplasmosis in Romanian patients diagnosed with schizophrenia and may serve as a foundation for future research and the development of preventive strategies.

Evaluation of toxoplasma IgG avidity results and their correlation with toxoplasma Gondii IgM and IgG, in pregnant women during the first trimester at American Hospital, International Hospital and Cambridge Clinical Laboratories, Albania

Evaluation of toxoplasma IgG avidity results and their correlation with toxoplasma Gondii IgM and IgG, in pregnant women during the first trimester at American Hospital, International Hospital and Cambridge Clinical Laboratories, Albania

Seroprevalence of Toxoplasma gondii among HIV Positive Patients under Surveillance in Greek Infectious Disease Units: A Screening Study with Comparative Evaluation of Serological Methods.

This study aims to screen for IgG antibodies against Toxoplasma gondii (T. gondii) in the sera of 155 newly diagnosed Human Immunodeficiency Virus (HIV) positive patients under surveillance in Greek Infectious Disease Units. Additionally, risk factors based on patient demographics were examined, and a comparative evaluation of commercially available serological methods was conducted. Three methods were employed to detect IgG antibodies against T. gondii: Enzyme-Linked Immunosorbent Assay (ELISA), Indirect Immunofluorescence Antibody Test (IFAT), and Western Blot (WB), which was used as a reference here. Forty-nine sera samples were true-positive for IgG antibodies against T. gondii, resulting in a 31.61% positivity rate, and the immunoassay test statistical reliability analysis resulted in higher IFAT accuracy (90.97%) compared to ELISA (76.26%). Furthermore, statistical analysis of demographic and immunological data included in the study placed female and foreign/non-Greek individuals at 2.24 (p = 0.0009) and 2.34 (p = 0.0006) times higher risk of positive T. gondii IgG testing compared to their male and Greek counterparts, respectively. Our findings on positivity rates and comparative serology underscore the importance of early and suitable screening measures for newly diagnosed HIV+ patients to mitigate the life-threatening outcomes that may arise from a potential subsequent T. gondii activation.

Seromolecular study on the prevalence and risk factors of Toxoplasma gondii infection in pregnant women referred to a gynecology hospital in Urmia, northwest part of Iran in 2022

Toxoplasmosis is a frequent infection among the human population. The infection can cause devastating complications for the fetus during pregnancy. The present study aimed to determine the serological and molecular prevalence of the infection and molecular characterization of Toxoplasma gondii isolates among pregnant women referred to Kowsar Hospital, Urmia, Iran. In a cross-sectional study, 340 blood samples were collected from pregnant women referred to Kowsar Hospital, Urmia, Iran from May to July 2022. Anti-T. gondii IgG and IgM seropositivity were determined by enzyme-linked immunosorbent assay. PCR was carried out by targeting the GRA6 gene of the parasite on all patients’ buffy coats. Anti-T. gondii IgG and IgM antibodies were positive in two (0.6%) women, and 101 (29.7%) women had anti-T. gondii IgG and 70.3% were seronegative. PCR was positive in two IgM-positive women, and both isolates belonged to T. gondii carrying the GRA6 allele of lineage I. The risk of infection was significantly higher in women who had constant contact with cats and soil, and who were residents of rural areas. The two IgM-positive women were asymptomatic regarding acute toxoplasmosis. According to the results of the present study, the prevalence of toxoplasmosis in pregnant women in Urmia is similar to its prevalence in other areas in northwestern Iran, and despite the low prevalence of acute infection, it should not be ignored.

Toxoplasma gondii Infection in Humans: A Comprehensive Approach Involving the General Population, HIV-Infected Patients and Intermediate-Duration Fever in the Canary Islands, Spain.

A prior investigation in 1993 identified a high seroprevalence of toxoplasmosis (63%) in the Canary Islands. This study aims to assess the current prevalence of the disease in diverse population groups. The study was based on a population-scale screening involving 273 residents utilizing T. gondii IgG ELISA and a 20 year retrospective study (1998-2018). This included AIDS/HIV outpatients (1357, of which 324 were residents), AIDS/HIV hospitalized patients (741) and patients with fever of intermediate duration (158). The seroprevalence in the resident population was 37%, with significant differences between islands. Among resident outpatients with AIDS/HIV, 14.2% had specific anti-T. gondii IgG, and three had anti-T. gondii IgM; however, IgG avidity testing indicated non-active infection. In patients hospitalized for AIDS/HIV, T. gondii causing encephalitis was detected in 2%. Among patients with fever of intermediate duration, 28.5% were positive for T. gondii IgG, and four also showed IgM positivity, although the infection was non-active. The study reveals a decrease in human toxoplasmosis over the past 30 years. However, the current seroprevalence, which stands at 37%, together with the substantial risk that T. gondii represents for immunocompromised individuals, highlights the need to implement preventive and control strategies to control the threat that this infection can pose to public health in the Canary Islands population.

Alterations in gut microbiota contribute to cognitive deficits induced by chronic infection of Toxoplasma gondii

Chronic infection with Toxoplasma gondii (T. gondii) emerges as a risk factor for neurodegenerative diseases in animals and humans. However, the underlying mechanisms are largely unknown. We aimed to investigate whether gut microbiota and its metabolites play a role in T. gondii-induced cognitive deficits. We found that T. gondii infection induced cognitive deficits in mice, which was characterized by synaptic ultrastructure impairment and neuroinflammation in the hippocampus. Moreover, the infection led to gut microbiota dysbiosis, barrier integrity impairment, and inflammation in the colon. Interestingly, broad-spectrum antibiotic ablation of gut microbiota attenuated the adverse effects of the parasitic infection on the cognitive function in mice; cognitive deficits and hippocampal pathological changes were transferred from the infected mice to control mice by fecal microbiota transplantation. In addition, the abundance of butyrate-producing bacteria and the production of serum butyrate were decreased in infected mice. Interestingly, dietary supplementation of butyrate ameliorated T. gondii-induced cognitive impairment in mice. Notably, compared to the healthy controls, decreased butyrate production was observed in the serum of human subjects with high levels of anti-T. gondii IgG. Overall, this study demonstrates that gut microbiota is a key regulator of T. gondii-induced cognitive impairment.

Prevalence of Toxoplasma gondii IgG Antibodies in Psychiatric Patients from Western Romania

IntroductionToxoplasma gondii, a ubiquitous protozoan parasite, has been previously associated with psychiatric disorders.ObjectivesTo assess the prevalence of IgG antibodies against T. gondii in psychiatric patients from Western Romania.MethodsWe included 464 psychiatric patients admitted to the Psychiatric Clinic, County Emergency Hospital of Arad, Western Romania. Clinical evaluation and laboratory tests were conducted in these patients, including serological tests to determine the presence of T. gondii IgG antibodies.ResultsOf the 464 psychiatric patients, 258 (55.5%) were residing in rural areas and 245 (52.7%) were female. T. gondii IgG antibodies were demonstrated in 325 (70.04%) of 464 study participants and the seroprevalence tended to increase with age.A significant higher T. gondii IgG seroprevalence was found in psychiatric patients aged between 40 to 59 years (p&lt;0.001) and in patients aged ≥60 years (p=0.001) compared to patients aged 19 to 39 years. A higher T. gondii IgG seroprevalence was determined in psychiatric patients residing in rural areas compared to those residing in urban areas (p=0.04). T. gondii IgG seroprevalence was higher in females compared to males (p=0.04).Assessment of seroprevalence by diagnostic revealed that T. gondii IgG antibodies were identified in 23 (85.19%) of 27 patients with delusional disorders, 24 (82.76%) of 29 with dementia, 51 (70.83%) of 72 with organic disorders, 75 (70.75%) of 106 with schizophrenia, 81 (70.43%) of 115 patients with depression, 17 (62.96%) of 27 with bipolar disorders, 27 (58.7%) of 46 with mood disorders and 7 (53.85%) of 13 with impulsive-control disorders.ConclusionsThe presence of T. gondii antibodies was demonstrated in a significant number of patients who attended the Psychiatric Clinic in Arad County, Western Romania. Results of this study suggest that T. gondii may be associated with several psychiatric disorders.Disclosure of InterestNone Declared

Seroprevalence and risk factors of Toxoplasma gondii in sheep and goats of North West Province, South Africa

BackgroundThe protozoan parasite Toxoplasma gondii causes toxoplasmosis, one of the most prevalent parasitic zoonotic diseases with significant economic and public health implications worldwide. Infection with the parasite has a significant adverse effect on sheep and goat production and can frequently go undetected in the herd, resulting in abortions and weak or dead offspring. Although there are few studies on seroprevalence and risk factors associated with T. gondii infections in livestock in other provinces of South Africa, there is no data in the North West province. Therefore, a cross-sectional study was conducted to investigate the seroprevalence of T. gondii and risk factors associated with exposure in sheep and goats of the North West province of South Africa. Sera from 439 livestock (164 sheep and 285 goats) were collected and analysed for the presence of T. gondii IgG antibodies using indirect ELISA (Enzyme-linked immunosorbent assay). An assessment of potential risk factors in farms associated with seropositivity was also conducted using a structured questionnaire.ResultsOut of the 439 tested sheep and goats, 13.9% (61/439) were positive for IgG antibodies against T. gondii. Sheep and goats had seroprevalences of 19.5% (32/164) and 10.5% (29/275) respectively. In the multivariable logistic regression model, the risk of acquiring T. gondii was significantly higher in the mixed breed [Odds ratio (OR) = 71.07; 95% confidence interval (CI): 266.8-1893.1; p < 0.011)] animals than white dorper sheep and in farms that burn or bury aborted material (OR = 42.04; CI: 179.9-982.5; p = 0.020) compared to those that only burn aborted material. The risk was lower for the farms in Kagisano-Molopo (OR = 0.00; CI: 0.0-25.4; p = 0.015) and Mahikeng (OR = 0.00; CI: 0.0-4.9; p < 0.001) local municipalities than Greater Taung local municipality, and for the animals that drink water from dams (OR = 0.03; CI: 0.2–58.8; p = 0.021) than those that drink from boreholes.ConclusionThe seroprevalence and risk factors associated with transmission observed show that T. gondii infection is widespread in sheep and goats of the North West province.

A public health problem in pregnant women: acute toxoplasma infection - a retrospective study of 12 years of data from a training and research hospital in Izmir

Introduction: The aim of this study is to identify the prevalence of acute toxoplasmosis in pregnant patients who are monitored in our hospital and to provide insight into screening and management options by assessing maternal and fetal outcomes. Methods: Pregnants between the ages of 18-49, who were followed up in the Gynecology and Obstetrics Polyclinic/Clinic and Infectious Diseases Polyclinic/Clinic of a Training and Research Hospital between January 1, 2010 and December 31, 2021, were included in this study. T. gondii IgM, IgG and avidity tests which were performed for pregnant women were evaluated retrospectively. Results: The study consisted of 7480 pregnants. Three hundred eighty-six (%5.16) of the pregnant women tested positive. Anti-T. gondii IgM seropositivity was found to be most common in the 18-28 age group (7.82%), and this frequency gradually decreased as age progressed. Two of the 122 patients whose polymerase chain reaction for T. gondii was examined from amniotic fluid at the outer center had congenital toxoplasmosis, and these pregnancies were terminated. Conclusions: Pregnant women continue to have a considerable risk of developing acute toxoplasmosis. For this infestation, which still remains on the agenda as a public health problem, every individual who is planning a pregnancy and has not had a serological test before should be screened before pregnancy. Pregnant women should continue to be informed and educated about this parasite, in our opinion.

Seroprevalence of Toxoplasma gondii Antibodies and Associated Risk Factors Among Women in Zakho City, Iraq.

Toxoplasmosis, an infectious disease caused by the obligate intracellular protozoan parasite, poses varying degrees of risk, ranging from asymptomatic cases in immunocompetent individuals to severe, life-threatening conditions in immunocompromised individuals and developing fetuses, especially when infection occurs during early pregnancy. While the disease is endemic in Iraq, there is a notable lack of precise information regarding its seroprevalence among females of childbearing age and pregnant women, along with associated risk factors in the Zakho district. This cross-sectional study aimed to address this gap by determining the prevalence of anti-Toxoplasma gondii IgG and IgM antibodies using the ELISA assay. The study involved 610 females aged 18-79 years from various residential areas within Zakho district, Iraq. The findings revealed a seroprevalence of 32.46% for anti-Toxoplasma IgG antibodies and 8.86% for IgM antibodies. Significant variations in IgG antibody seroprevalence were observed across different age groups (P=0.008), with the highest prevalence noted among those aged 46-55 years (47.73%). Conversely, IgM antibody seroprevalence, while non-significant (P>0.05), displayed the highest rate of 10.05% among ages 18-25 years. The study identified residence as a variable significantly associated with toxoplasmosis. Additionally, contact with cats, marital status, a history of abortion, and the consumption of homemade food showed significant associations with anti-Toxoplasma IgM antibodies only. These findings strongly suggest that Toxoplasma gondii is a prevalent causative agent of infection in Zakho city, Iraq. This study contributes valuable insights into the seroprevalence and associated risk factors, providing a foundation for targeted interventions and further research in this region.

Seroepidemiological study on coinfection of toxoplasmosis and active tuberculosis in Northern Iran: a case control study.

Coinfection of tuberculosis (TB) and human parasitic infections is common in developing countries. There is little information about the prevalence of Toxoplasma gondii (T. gondii) infection among TB patients in Iran. In this case-control study, anti-toxoplasma antibodies were measured by ELISA method in 100 patients with active tuberculosis and 100 healthy individuals who were matched in terms of sex, age, and place of residence. Anti-T. gondii IgG antibodies were diagnosed in 62% of TB patients (95% CI 53-71%) and 70% of control subjects (95% CI 62-78%). Anti-T. gondii IgM antibodies were found in 1% of both TB patients and control group. The seroprevalence of T. gondii infection was not significantly different between TB patients and healthy individuals (P > 0.05). None of the assessed sociodemographic and behavioral factors was recognized as a risk factor for toxoplasmosis in TB infected patients. Moreover, the level of anti-T. gondii IgG antibodies concentration in TB patients was significantly higher than in control subjects and revealed skewness towards humoral immune response in TB patients. Coinfection of toxoplasmosis and tuberculosis was prevalent but T. gondii infection was independent of active TB in this co-endemic area.