Abstract Textile dyes are artificial dyes that are widely used by the textile industry because they have many types of colors and are cheap. The azo dye used can be an aromatic compound that has stable properties, so if it is in water, it can be more toxic than the azo dye itself. This study aims to determine the effect of exposure to acid red 88 dye and a minimum dose of acid red 88 dye on the level of gonad maturity and gonadosomal index (GSI) of parent tilapia fish. This research was an experimental study; tilapia fish were kept for 7 days with 5 treatments and 4 repetitions. Data collection was carried out by looking at the TKG of tilapia fish in morphology and histology, and then the GSI of tilapia fish was analyzed using the ANOVA test. The results of this research show that acid red 88 is said to be an endocrine-disrupting chemical and produces excess reproductive hormones, which can affect fish health. Then there was damage to the histology of the TKG of the tilapia. The conclusion of this research is that exposure and dose of acid red 88 dye have an influence on the morphology and histology of TKG and the GSI value of tilapia.