Glyphosate Resistance Research Articles

Narrow-row soybean and a cereal rye cover crop suppress glyphosate-resistant horseweed (Conyza canadensis)

AbstractAlternative strategies are needed for management of glyphosate-resistant (GR) horseweed in soybean. Integrating a cereal rye cover crop with soybean planted in narrow rows may improve control and reduce herbicide selection pressure for herbicide-resistant horseweed biotypes. Four site-years of experiments were conducted in Michigan to determine whether fall-planted cereal rye terminated with glyphosate 1 wk prior to (early termination) or 1 wk after (planting green) planting in combination with narrow-row soybean improved GR horseweed management. At postemergence (POST) herbicide application, horseweed biomass was reduced by 71% to 90% when soybean was planted into cereal rye, regardless of termination time, compared with no cover across all row widths. Planting green or narrow-row soybean suppressed horseweed through soybean harvest. When glyphosate was applied POST (noneffective), horseweed biomass was 36% to 46% lower when planting green compared with early terminated cereal rye and no cover. Similarly, planting soybean in 19- and 38-cm rows reduced horseweed biomass by 48% and 28%, respectively, compared with 76-cm rows. Cereal rye did not affect soybean yield pooled over 3 of 4 site-years; however, narrow row soybean yielded 11% to 18% higher than 76-cm rows. Soybean yield was 11% higher when an effective POST herbicide was applied. In conclusion, fall-seeded cereal rye or narrow-row soybean suppressed horseweed compared with no cover and 76-cm rows; however, the effects of early termination did not last throughout the growing season in most cases. Delaying cover crop termination by planting green reduced horseweed biomass and density through soybean harvest, but reduced yield in 1 site-year due to an increased incidence of white mold. These cultural practices have a positive influence on suppressing horseweed that should be part of an overall horseweed management strategy; however, the use of an effective POST herbicide is still needed for complete season-long horseweed management.

In-field assessment of EPSPS amplification on fitness cost in mixed glyphosate-resistant and glyphosate-sensitive populations of Palmer amaranth (Amaranthus palmeri)

AbstractComparing fitness of herbicide-resistant and herbicide-susceptible weed biotypes is important for managing herbicide resistance. Previous research suggests there is little to no fitness penalty from amplification of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene (a mechanism of glyphosate resistance) in Palmer amaranth (Amaranthus palmeri S. Watson) in controlled studies in the greenhouse or growth chamber. A field study was conducted in North Carolina at three locations naturally infested with A. palmeri to determine vegetative, reproductive, and germination fitness of plants with and without EPSPS amplification grown season-long with cotton (Gossypium hirsutum L.). Seed number was not correlated with EPSPS copy number. However, when plants were binned into two groups, those having an EPSPS copy number ≥2 (relative to reference genes) and those having an EPSPS copy number <2, plant fresh weight and seed number were 1.4 and 1.6 times greater, respectively, for plants with fewer than 2 EPSPS copies. Amaranthus palmeri height and seed germination, and yield of cotton, did not differ when comparing the two binned groups. These data suggest that A. palmeri plants with EPSPS amplification are relatively less fit in the absence of glyphosate, but this reduced fitness does not translate into differences in interference with cotton.

Distribution and genetic characterization of bird rape mustard (Brassica rapa) populations and analysis of glyphosate resistance introgression.

The introgression of a transgene conferring glyphosate resistance from Brassica napus (rapeseed, canola) to Brassica rapa weeds (bird rape) was documented at a single location in 2007. In 2015, several cases of glyphosate resistant mustard were reported by growers in areas where rapeseed was seldom grown. Survey result indicated glyphosate resistant bird rape mustard is present in areas where glyphosate tolerant corn and soybean are often grown in rotation. Genetic analyses reveal that hybridization followed by introgression and progressive loss of chromosome is the likely mechanism for the horizontal gene transfer (HGT) of glyphosate resistance. Introgression of the glyphosate-resistance conferring transgene in the populations studied appears to have occurred several times, consistent with the ease for B. rapa to form hybrids with B. napus. The introduction of a transgene into a crop should therefore take into account the weediness of the species that share a common genome and their ability to form hybrids. We provide here such an example between B. napus and B. rapa, and potentially between B. napus and Raphanistrum raphanistrum. © 2022 Her Majesty the Queen in Right of Canada. Pest Management Science © 2022 Society of Chemical Industry. Reproduced with the permission of the Minister of Agriculture and Agri-Food Canada.

Quizalofop-resistant rice response to quizalofop when exposed to low rates of glyphosate and imazethapyr

AbstractInjury to quizalofop-resistant rice was reported in some fields following postemergence applications of quizalofop. Glyphosate-resistant (GR) corn, cotton, and soybean, and imidazolinone-resistant rice are grown near quizalofop-resistant rice. Herbicide drift from glyphosate and imazethapyr and the resulting crop injury and potential yield loss is a cause of concern for producers. Field experiments conducted near Colt, and Keiser, AR, in 2021 evaluated whether low rates of glyphosate or imazethapyr interact with sequential quizalofop applications to exacerbate injury to quizalofop-resistant rice compared to quizalofop applications alone. Herbicide treatments consisted of a low rate of glyphosate (90 g ae ha−1) or imazethapyr (10.7 g ai ha−1) applied 10, 7, 4, and 0 d before the 2-leaf growth stage of rice, and glyphosate or imazethapyr, at the same rate and timings, followed by quizalofop at 120 g ai ha−1 applied to 2-leaf rice. All plots treated with quizalofop received a subsequent application of the same herbicide and rate at the 5-leaf rice stage. At 28 d after final treatment (DAFT), glyphosate followed by quizalofop the same day to 2-leaf rice caused 77% injury compared with 58% when glyphosate was applied alone, regardless of location. Glyphosate followed by quizalofop the same day reduced rough rice grain yield by 67% compared with 33% when glyphosate was applied alone to 2-leaf rice at the Colt location. Application of imazethapyr followed by quizalofop the same day to 2-leaf rice caused more injury (63% and 19% injury at the Colt and Keiser locations, respectively) than imazethapyr alone (42% and 7% injury at the Colt and Keiser locations, respectively) at 35 DAFT. Overall, glyphosate and imazethapyr followed by quizalofop applications worsened injury compared to glyphosate, imazethapyr, and quizalofop applications alone. As the interval between exposure to a low rate of glyphosate or imazethapyr and quizalofop decreases, the detrimental effect of herbicide on rice likewise increases.

Economic impact of glyphosate-resistant weeds on major field crops grown in Ontario

AbstractLimited information exists on the global economic impact of glyphosate-resistant (GR) weeds. The objective of this manuscript was to estimate the potential yield and economic loss from uncontrolled GR weeds in the major field crops grown in Ontario, Canada. The impact of GR weed interference on field crop yield was determined using an extensive database of field trials completed on commercial farms in southwestern Ontario between 2010 and 2021. Crop yield loss was estimated by expert opinion (weed scientists and Ontario government crop specialists) when research data were unavailable. This manuscript assumes that crop producers adjust their weed management programs to control GR weeds, which increases weed management costs but reduces crop yield loss from GR weed interference by 95%. GR volunteer corn, horseweed, waterhemp, giant ragweed, and common ragweed would cause an annual monetary loss of (in millions of Can$) $172, $104, $11, $3, and $0.3, respectively, for a total annual loss of $290 million if Ontario farmers did not adjust their weed management programs to control GR biotypes. The increased herbicide cost to control GR volunteer corn, horseweed, waterhemp, giant ragweed, and common ragweed in the major field crops in Ontario is estimated to be (in millions of Can$) $17, $9, $2, $0.1, and $0.02, respectively, for a total increase in herbicide expenditures of $28 million annually. Reduced GR weed interference with the adjusted weed management programs would reduce farm-gate monetary crop loss by 95% from $290 million to $15 million. This study estimates that GR weeds would reduce the farm-gate value of the major field crops produced in Ontario by Can$290 million annually if Ontario farmers did not adjust their weed management programs, but with increased herbicide costs of Can$28 million and reduced crop yield loss of 95% the actual annual monetary loss in Ontario is estimated to be Can$43 million annually.

Sequence characterization of eccDNA content in glyphosate sensitive and resistant Palmer amaranth from geographically distant populations.

The discovery of non-chromosomal circular DNA offers new directions in linking genome structure with function in plant biology. Glyphosate resistance through EPSPS gene copy amplification in Palmer amaranth was due to an autonomously replicating extra-chromosomal circular DNA mechanism (eccDNA). CIDER-Seq analysis of geographically distant glyphosate sensitive (GS) and resistant (GR) Palmer Amaranth (Amaranthus palmeri) revealed the presence of numerous small extra-chromosomal circular DNAs varying in size and with degrees of repetitive content, coding sequence, and motifs associated with autonomous replication. In GS biotypes, only a small portion of these aligned to the 399 kb eccDNA replicon, the vehicle underlying gene amplification and genetic resistance to the herbicide glyphosate. The aligned eccDNAs from GS were separated from one another by large gaps in sequence. In GR biotypes, the eccDNAs were present in both abundance and diversity to assemble into a nearly complete eccDNA replicon. Mean sizes of eccDNAs were similar in both biotypes and were around 5kb with larger eccDNAs near 25kb. Gene content for eccDNAs ranged from 0 to 3 with functions that include ribosomal proteins, transport, metabolism, and general stress response genetic elements. Repeat content among smaller eccDNAs indicate a potential for recombination into larger structures. Genomic hotspots were also identified in the Palmer amaranth genome with a disposition for gene focal amplifications as eccDNA. The presence of eccDNA may serve as a reservoir of genetic heterogeneity in this species and may be functionally important for survival.

Stacking herbicide detoxification and resistant genes improves glyphosate tolerance and reduces phytotoxicity in tobacco (Nicotiana tabacum L.) and rice (Oryza sativa L.)

Glyphosate residues retained in the growing meristematic tissues or in grains of glyphosate-resistant crops affect the plants physiological functions and crop yield. Removing glyphosate residues in the plants is desirable with no penalty on crop yield and quality. We report a new combination of scientific strategy to detoxify glyphosate that reduces the residual levels and improve crop resistance. The glyphosate detoxifying enzymes Aldo-keto reductase (AKR1) and mutated glycine oxidase (mGO) with different modes of action were co-expressed with modified EPSPS, which is insensitive to glyphosate in tobacco (Nicotiana tabacum L.) and rice (Oryza sativa L.). The transgenic tobacco plants expressing individual PsAKR1, mGO, CP4-EPSPS, combinations of PsAKR1:CP4EPSPS, PsAKR1:mGO, and multigene with PsAKR1: mGO: CP4EPSPS genes were developed. The bio-efficacy studies of in-vitro leaf regeneration on different concentrations of glyphosate, seedling bioassay, and spray on transgenic tobacco plants demonstrate that glyphosate detoxification with enhanced resistance. Comparative analysis of the transgenic tobacco plants reveals that double and multigene expressing transgenics had reduced accumulation of shikimic acid, glyphosate, and its primary residue AMPA, and increased levels of sarcosine were observed in all PsAKR1 expressing transgenics. The multigene expressing rice transgenics showed improved glyphosate resistance with yield maintenance. In summary, results suggest that stacking genes with two different detoxification mechanisms and insensitive EPSPS is a potential approach for developing glyphosate-resistant plants with less residual content.

Control of glyphosate-resistant horseweed with Group 4 herbicides in soybean

AbstractLittle information is available on the relative efficacy of Group 4 herbicides for glyphosate-resistant (GR) horseweed management in soybean. Five field research experiments were conducted in growers’ fields from 2020 to 2021 to determine GR horseweed control with Group 4 herbicides applied preplant (PP) alone and in a mixture. There was minimal soybean injury (≤4%) with herbicides evaluated. Dicamba, 2,4-D, or halauxifen-methyl applied PP controlled GR horseweed 92% to 96%, 73% to 76%, and 85% to 89%, respectively. The mixtures of dicamba + 2,4-D, dicamba + halauxifen-methyl and dicamba + 2,4-D + halauxifen-methyl provided 97% to 99% control of GR horseweed, similar to dicamba applied alone. The mixture of 2,4-D + halauxifen-methyl provided 93% to 94% control of GR horseweed. Dicamba + saflufenacil controlled GR horseweed at 98%. Dicamba alone, dicamba + 2,4-D ester, dicamba + halauxifen-methyl, and dicamba + 2,4-D ester + halauxifen-methyl decreased GR horseweed density 97%, 99%, 99%, and 98%, respectively, similar to a 98% density reduction with dicamba + saflufenacil. Other herbicide treatments had no effect on GR horseweed density. Dicamba, 2,4-D, and halauxifen-methyl applied PP decreased GR horseweed dry biomass by 99%, 76%, and 72%, respectively. The mixtures of dicamba + 2,4-D, dicamba + halauxifen-methyl, and dicamba + 2,4-D + halauxifen-methyl decreased GR horseweed dry biomass by 99% to 100%, similar to a 99% dry biomass reduction with dicamba + saflufenacil. The mixture of 2,4-D + halauxifen-methyl decreased GR horseweed dry biomass by 94%. Soybean yield was decreased by 61% when GR horseweed was left uncontrolled. Results show that Group 4 herbicides that include dicamba applied PP can be very effective in managing GR horseweed in soybean.

Challenging glyphosate resistance EPSPS P106S and TIPS mutations with soybean competition and glyphosate: implications for management.

Eleusine indica (L.) Gaertn. (goosegrass) is a major weed in global cropping systems. It has evolved resistance to glyphosate due to single Pro-106-Ser (P106S) or double Thr-102-Ile + Pro-106-Ser (TIPS) EPSPS target site mutations. Here, experiments were conducted to evaluate the single effect of soybean competition and its combined effect with a glyphosate field dose (1080 g ae ha-1 ) on the growth and fitness of plants carrying these glyphosate resistance endowing target site mutations. TIPS E.indica plants are highly glyphosate-resistant but the double mutation endows a substantial fitness cost. The TIPS fitness penalty increased under the effect of soybean competition resulting in a cost of 95%, 95% and 96% in terms of, respectively, vegetative growth, seed mass and seed number investment. Glyphosate treatment of these glyphosate-resistant TIPS plants showed an increase in growth relative to those without glyphosate. Conversely, for the P106S moderate glyphosate resistance mutation, glyphosate treatment alone reduced survival rate, vegetative growth, aboveground biomass (34%), seed mass (48%) and number (52%) of P106S plants relative to the glyphosate nontreated plants. However, under the combined effects of both soybean competition and the field-recommended glyphosate dose, vegetative growth, aboveground biomass, seed mass and number of P106S and TIPS plants were substantially limited (by ≤99%). The ecological environment imposed by intense competition from a soybean crop sets a significant constraint for the landscape-level increase of both the E.indica single and double glyphosate resistance mutations in the agroecosystem and highlights the key role of crop competition in limiting the population growth of weeds, whether they are herbicide-resistant or susceptible. © 2022 Society of Chemical Industry.

Effect of cotton herbicide programs on weed population trajectories and frequency of glyphosate-resistant Palmer amaranth (Amaranthus palmeri)

AbstractThe adoption of dicamba-resistant cotton (Gossypium hirsutum L.) cultivars allows using dicamba to reduce weed populations across growing seasons. However, the overuse of this tool risks selecting new herbicide-resistant biotypes. The objectives of this research were to determine the population trajectories of several weed species and track the frequency of glyphosate-resistant (GR) Palmer amaranth (Amaranthus palmeri S. Watson) over 8 yr in dicamba-resistant cotton. An experiment was established in North Carolina in 2011, and during the first 4 yr, different herbicide programs were applied. These programs included postemergence applications of glyphosate, alone or with dicamba, with or without residual herbicides. During the last 4 yr, all programs received glyphosate plus dicamba. Biennial rotations of postemergence applications of glyphosate only and glyphosate plus dicamba postemergence with and without preemergence herbicides were also included. Sequential applications of glyphosate plus dicamba were applied to the entire test area for the final 4 yr of the study. No herbicide program was entirely successful in controlling the weed community. Weed population trajectories were different according to species and herbicide program, creating all possible outcomes; some increased, others decreased, and others remained stable. Density of resistant A. palmeri increased during the first 4 yr with glyphosate-only programs (up to 11,739 plants m−2) and decreased a 96% during the final 4 yr, when glyphosate plus dicamba was implemented. This species had a strong influence on population levels of other weed species in the community. Goosegrass [Eleusine indica (L.) Gaertn.] was not affected by A. palmeri population levels and even increased its density in some herbicide programs, indicating that not only herbicide resistance but also reproductive rates and competitive dynamics are critical for determining weed population trajectories under intensive herbicide-based control programs. Frequency of glyphosate resistance reached a maximum of 62% after 4 yr, and those levels were maintained until the end of the experiment.

Determination of Glyphosate Resistant Horseweed (Conyza Canadensis (L.) Cronquist) in Apple Orchard in Isparta

Türkiye’nin önemli elma üretim bölgelerinden biri de Isparta’dır. Elma üretim alanlarında sorunlara neden olan hastalık, zararlı ve yabancı otlar elma üretimini önemli derecede sınırlamaktadır. Yabancı otların doğrudan oluşturduğu zarar sınırlı kalmakla birlikte, özellikle erken gelişme döneminde elma bitkileriyle su, besin maddesi ve ışık rekabetine girdikleri için önemlidir. Söz konusu kayıplarının azaltılması için en çok başvurulan teknik herbisit kullanımıdır. Herbisitler, aynı alanda rotasyon yapılmadan uzun süre kullanıldığında dayanıklı yabancı ot popülasyonları görülebilir. Herbisite dayanıklı popülasyonların görülmesinden sonra uygulanacak dayanıklılık yönetimi daha uzun ve kapsamlı çalışmaları kapsar. Bu çalışma, Isparta elma üretim alanlarında glyphosate’a dayanıklı pire otu (Conyzacanadensis) popülasyonlarının belirlenmesi için 2015-2019 yıllarında yürütülmüştür. Dayanıklılığın tespitinde bioassay yöntemi kullanılmıştır. Tarama testi kapsamında iklim odası koşullarında yetiştirilen pire otu bitkilerine Glyphosate’ın tavsiye edilen dozu uygulanmış ve bitkilerin Glyphosate’a dayanıklılık durumları belirlenmiştir. Tarama testi sonunda dayanıklılık şüphesi taşıyan 2 Pire otu popülasyonu ile hassas popülasyon doz-tepki denemesine alınmıştır. Doz-tepki denemelerinde Pire otu popülasyonlarına Glyphosate’ın logaritmik dozları uygulanmış ve etki non-linear regresyon analizi ile belirlenmiştir. Glyphosate’a dayanıklılık şüphesi taşıyan popülasyonların I50 değerlerinin F1 popülasyonlarında96.087 ve 83.126mlticari doz/da, F2 popülasyonlarında ise106.48 ve 98.82ml ticari doz/da bulunmuştur. Dayanıklılık şüphesi taşıyan şifa otu popülasyonlarının Glyohosate’a toleranslarının yüksek olduğu değerlendirilmektedir.

A Highly Glyphosate-Resistant EPSPS Mutant from Laboratory Evolution

EPSP synthase is the target enzyme of glyphosate herbicides. Due to the extensive use of glyphosate, it is very important to obtain EPSPS genes with high glyphosate resistance for the development of transgenic crops. GR79-EPSPS is a class I EPSP synthase with certain glyphosate resistance isolated from glyphosate-contaminated soil. After more than 1000 generations, a Y40I substitution was identified, and the enzyme had a nearly 1.8-fold decrease in Km [PEP] and a 1.7-fold increase in Ki[glyphosate] compared to the wild-type enzyme. Enzyme dynamics and molecular dynamics analysis showed that the substitution was near the hinge region of EPSPS, and the affinity of glyphosate binding to amino acid residues of the active site decreased due to Y40I substitution, resulting in an increase in glyphosate resistance. These results provide more evidence for the combination of directed evolution and rational design of protein engineering.

Economics of reducing Palmer amaranth seed production in dicamba/glufosinate/glyphosate‐resistant soybean

AbstractIncreased prevalence of glyphosate‐resistant (GR) weeds within agronomic cropping systems has led to the readoption of pre‐emergence (PRE) herbicides and use of multiple herbicide‐resistant soybean [Glycine max (L.) Merr.] cultivars. Herbicide programs were evaluated in the recently commercialized dicamba/glyphosate/glufosinate‐resistant (DGGR) soybean for weed control, reduction of Palmer amaranth (Amaranthus palmeri S. Watson) seed production, crop safety, and economic performance. At 35 days after pre‐emergence herbicides, acetochlor plus dicamba plus metribuzin, acetochlor/fomesafen plus dicamba, dicamba plus flumioxazin, and imazethapyr/pyroxasulfone/saflufenacil provided 80–99% control of velvetleaf (Abutilon theophrasti Medik.), Palmer amaranth, common lambsquarters (Chenopodium album L.), and Poaceae species. Evaluation at 14 days after early postemergence herbicides indicated PRE followed by (fb) POST applications of mixtures of acetochlor, dicamba, glufosinate, and glyphosate provided 80–99% weed control compared with 67–93% control in POST‐only programs. Most herbicide programs provided 83–99% control of grass and broadleaf weeds, with 85–91% weed biomass reductions at 28 days after late‐POST. The PRE fb POST programs reduced Palmer amaranth seed production by 94–99%, whereas POST‐only programs provided 75–83% reduction. In 2020, most programs provided gross profit margins ≥US$1,000 ha−1, with glufosinate fb glufosinate and imazethapyr/pyroxasulfone/saflufenacil fb acetochlor plus glufosinate providing $1,481 and $1,466 ha−1, respectively. Benefit/cost ratios ranged between 0.3 and 3.9 in 2019 due to hail but increased to 2.9–10.9 in 2020. Results of this study support use of PRE herbicides with multiple sites of action in DGGR soybean and indicate that glufosinate can provide POST control of GR Palmer amaranth.

Structural analysis and engineering of aldo-keto reductase from glyphosate-resistant Echinochloa colona

Glyphosate is a dominant organophosphate herbicide that inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) of the shikimate pathway. Glyphosate is extensively applied since manufactured, which has led to the emergence of various glyphosate-resistant crops and weeds. However, the molecular mechanism of many glyphosate-resistance machineries remains unclear. Recently, the upregulated expression of two homologous aldo-keto reductases (AKRs), designated as AKR4C16 and AKR4C17, were found to contribute to the glyphosate resistance in Echinochloa colona. This represents the first naturally evolved glyphosate-degrading machinery reported in plants. Here, we report the three-dimensional structure of these two AKR enzymes in complex with cofactor by performing X-ray crystallography. Furthermore, the binding-mode of glyphosate were elucidated in a ternary complex of AKR4C17. Based on the structural information and the previous study, we proposed a possible mechanism of action of AKR-mediated glyphosate degradation. In addition, a variant F291D of AKR4C17 that was constructed based on structure-based engineering showed a 70% increase in glyphosate degradation. In conclusion, these results demonstrate the structural features and glyphosate-binding mode of AKR4C17, which increases our understanding of the enzymatic mechanism of glyphosate bio-degradation and provides an important basis for the designation of AKR-based glyphosate-resistance for further applications.

A novel EPSPS Pro-106-His mutation confers the first case of glyphosate resistance in Digitaria sanguinalis.

Digitaria sanguinalis has been identified as a species at high risk of evolving herbicide resistance, but thus far, there are no records of resistance to glyphosate. This weed is one of the most common weeds of summer crops in extensive cropping areas in Argentina. It shows an extended period of seedling emergence with several overlapping cohorts during spring and summer, and is commonly controlled with glyphosate. However, a D.sanguinalis population was implicated as a putative glyphosate-resistant biotype based on poor control at recommended glyphosate doses. The field-collected D.sanguinalis population (Dgs R) from the Rolling Pampas has evolved glyphosate resistance. Differences in plant survival and shikimate levels after field-recommended and higher glyphosate doses were evident between Dgs R and the known susceptible (Dgs S) population; the resistance index was 5.1. No evidence of differential glyphosate absorption, translocation, metabolism or basal EPSPS activity was found between Dgs S and Dgs R populations; however, a novel EPSPS Pro-106-His point substitution is probably the primary glyphosate resistance-endowing mechanism. EPSPS in vitro enzymatic activity demonstrated that an 80-fold higher concentration of glyphosate is required in Dgs R to achieve similar EPSPS activity inhibition to that in the Dgs S population. This study reports the first global case of glyphosate resistance in D.sanguinalis. This unlikely yet novel transversion at the second position of the EPSPS 106 codon demonstrates the intensity of glyphosate pressure in selecting unexpected glyphosate resistance alleles if they retain EPSPS functionality. © 2022 Society of Chemical Industry.

History of Herbicide-Resistant Traits in Cotton in the U.S. and the Importance of Integrated Weed Management for Technology Stewardship.

This paper reviews the history of herbicide-resistant (HR) traits in U.S. cotton since the beginning, highlighting the shortcomings of each trait over time that has led to the development of their successor and emphasizing the importance of integrated weed management (IWM) going forward to ensure their long-term sustainability. Introduction of glyphosate-resistant cropping systems has allowed for expansion of no-till systems more reliant on herbicides, favored less diverse crop rotations, and heavily relied on a single herbicide mode of action (MOA). With repeated applications of glyphosate over the years, biotypes of glyphosate-resistant (GR) A. palmeri and other weeds became economically damaging pests in cotton production systems throughout the U.S. Moreover, the reported cases of weeds resistant to different MOA across various parts of the United States has increased. The dicamba- (XtendFlex®) and 2,4-D-resistant (Enlist®) cotton traits (with stacks of glyphosate and glufosinate resistance) were introduced and have been highly adopted in the U.S. to manage HR weeds. Given the current rate of novel herbicide MOA discovery and increase in new HR weed cases, the future of sustainable weed management relies on an integrated approach that includes non-herbicidal methods with herbicides to ensure long-term success.

Functional characterization of differentially-expressed increased glyphosate resistance (igrA) gene in transgenic rice (Oryza sativa L.)

Functional characterization of differentially-expressed increased glyphosate resistance (igrA) gene in transgenic rice (Oryza sativa L.)

Dynamic Seed Emission, Dispersion, and Deposition from Horseweed (Conyza canadensis (L.) Cronquist).

The wide dispersion of glyphosate-resistant (GR) horseweed (Conyza canadensis (L.) Cronquist: synonym Erigeron canadensis L.) biotypes has been reported in agricultural fields in many states. GR traits may be transferred through seeds or pollen from fields with existing GR horseweed prevalence to surrounding fields. Understanding seed production and movement is essential when characterizing and predicting the spread of GR horseweed, yet a literature review indicates that there are no experimental data on dynamic (hourly) seed production and horizontal dispersion and deposition from horseweed. To obtain the dynamic data, two field experiments were performed, one in Illinois and one in Tennessee, USA in 2013 and 2014, respectively. Seed concentration and deposition along with atmospheric conditions were measured with samplers in the Illinois (184 m × 46 m, natural plants, density = 9.5 plants/m2) and Tennessee (6 m × 6 m, cultivated plants, density = 4 plants/m2) experimental fields and their surrounding areas along the downwind direction up to 1 km horizontally and 100 m vertically in the Illinois field and up to 32 m horizontally and 5 m vertically in the Tennessee field. The dynamic seed source strengths (emission rates) measured during two entire seed-shedding seasons were reported, ranging from 0 to 0.41 grains/plant/s for Illinois and ranging from 0 to 0.56 grains/plant/s for Tennessee. The average total seed production was an estimated 122,178 grains/plant for the duration of the Illinois experiment and 94,146 grains/plant for Tennessee. Seeds trapped by Rotorod samplers attached beneath two balloons in the Illinois field experiment were observed at heights of 80 to 100 m, indicating the possibility of long-distance transport. Normalized (by source data) seed deposition with distance followed a negative power exponential function. Seed emission and transport were affected mainly by wind speed. This study is the first to investigate dynamic horseweed seed emission, dispersion, and deposition for an entire seed-shedding season. The results will aid in the management of GR horseweed. The potential for regional effects of horseweed invasion may require all farmers to control horseweed in their individual fields.

Multiple ways to evade the bacteriostatic action of glyphosate in rhizobia include the mutation of the conserved serine 90 of the nitrogenase subunit NifH to alanine

The genome resequencing of spontaneous glyphosate-resistant mutants derived from the soybean inoculant E109 allowed identifying genes most likely associated with the uptake (gltL and cya) and metabolism (zigA and betA) of glyphosate, as well as with nitrogen fixation (nifH). Mutations in these genes reduce the lag phase and improve nodulation under glyphosate stress. In addition to providing glyphosate resistance, the amino acid exchange Ser90Ala in NifH increased the citrate synthase activity, growth rate and plant growth-promoting efficiency of E109 in the absence of glyphosate stress, suggesting roles for this site during both the free-living and symbiotic growth stages.

Engineering tropane alkaloid production and glyphosate resistance by overexpressing AbCaM1 and G2-EPSPS in Atropa belladonna

Atropa belladonna is an important industrial crop for producing anticholinergic tropane alkaloids (TAs). Using glyphosate as selection pressure, transgenic homozygous plants of A. belladonna are generated, in which a novel calmodulin gene (AbCaM1) and a reported EPSPS gene (G2-EPSPS) are co-overexpressed. AbCaM1 is highly expressed in secondary roots of A. belladonna and has calcium-binding activity. Three transgenic homozygous lines were generated and their glyphosate tolerance and TAs’ production were evaluated in the field. Transgenic homozygous lines produced TAs at much higher levels than wild-type plants. In the leaves of T2GC02, T2GC05, and T2GC06, the hyoscyamine content was 8.95-, 10.61-, and 9.96 mg/g DW, the scopolamine content was 1.34-, 1.50- and 0.86 mg/g DW, respectively. Wild-type plants of A. belladonna produced hyoscyamine and scopolamine respectively at the levels of 2.45 mg/g DW and 0.30 mg/g DW in leaves. Gene expression analysis indicated that AbCaM1 significantly up-regulated seven key TA biosynthesis genes. Transgenic homozygous lines could tolerate a commercial recommended dose of glyphosate in the field. In summary, new varieties of A. belladonna not only produce pharmaceutical TAs at high levels but tolerate glyphosate, facilitating industrial production of TAs and weed management at a much lower cost.