Glyphosate-containing Herbicide Research Articles

Economic efficiency of maize crop protection against weeds

The influence of agricultural practices for cultivating corn on the grain yield of this crop was stu- died. It has been shown that in the conditions of the central part of Belarus, with semi-steam tillage after harvesting the previous crop or cultivating stubble oilseed radish and using the herbicides Meister Power, MD (1.5 l/ha), Adengo, KS (0.4 l) in the 3-leaf phase of corn /ha) grain yield, net income and profitability were at the level of using the herbicide Gardo Gold, KS (4.0 l/ha) against the background of post-harvest application of the glyphosate-containing herbicide Volnik Super, BP (2.5 l/ha). At the same time, the herbicide load decreased from 3.38 to 0.09–0.19 kg/ha a. i., i. e., by 94.4–97.3 %, which is of impor- tant environmental significance.

Diverging fates of cadmium and glyphosate during pasta cooking

Durum wheat cultivars with varying abilities to accumulate cadmium were grown and treated in the field with a glyphosate-containing herbicide at different stages of maturity to produce grain with higher and lower concentrations of cadmium (0.066–0.214 mg/kg) and glyphosate (0.474–0.874 mg/kg). The grain was milled, and fractions were analysed for cadmium and glyphosate. The highest concentrations for both cadmium and glyphosate were associated with bran and shorts, although the percentage of total cadmium mass in bran (23–25%) was less than glyphosate (38%). The preparation of dried pasta from semolina and flour milling fractions reduced concentrations by a factor of 1.8 for glyphosate and 1.4 for cadmium. Dried pasta was cooked and analysed along with the cooking water for cadmium and glyphosate at seven-time points from 0 to 15 min. Concentrations of glyphosate in cooked pasta decreased significantly with cooking time; no decrease was observed for cadmium concentrations. Analysis of cooking water demonstrated that glyphosate migrated from pasta to the cooking water. After 15 min of cooking, approximately 73% of the total glyphosate mass had transferred from pasta to cooking water. Over the same time period, only 5% of the total cadmium mass had transferred from pasta to cooking water.

BIODEGRADATION OF GLYPHOSATE CONTAINING HERBICIDE BY SOIL FUNGI

Ten fungal isolates were isolated from two herbicide-contaminated soil farms obtained from Amoyo and the University of Ilorin environment in Kwara State after enrichment with mineral salt medium (MSM) supplemented with glyphosate-containing herbicide. The growth of fungal isolates was efficiently stimulated by the organophosphorus herbicide. Fungi isolated were subjected to screening by varying the herbicide concentrations from 0.1 to 3%, which is prepared with MSM. This screening showed that all the fungal isolates had the ability to act in the biodegradation process. However, varying degradative potentials were observed, as some had heavy growth while others had only slight or no growth as the concentrations of the herbicide increased. The ten fungal isolates were characterized and identified as Aspergillus niger, Penicillum spinulosum, Aspergillus terrus, Aspergillus flavus, Fusarium oxysporum, Mucor spp., Aspergillus oryzae, Aspergillus tamari, Rhizopus stolonifer, and Trichoderma koningii, and these were reduced to six after screening with 3% concentration of the herbicide. Four isolates (A. niger, F. oxysporum, Mucor spp., and A. flavus) were selected based on their growth ability on the medium during screening and were used in the biodegradation study. However, there is an increase in fungal dry weights ranging from 8.60 to 18% for 12 days. This shows that these fungi can be employed in the biodegradation of herbicides since they are potentially effective species and are environmentally safer alternative to protect the soil from the contamination of glyphosate-containing herbicide residues.

Influence of tillage systems on terbuthylazine degradation and leaching in the soil

The aim of the study was to evaluate the effect of the cultivation system on the rate of degradation and movement of terbuthylazine in the soil. Model experiments were carried out on soil taken from the field, parts of which were differentiated for 3 years in terms of the cultivation system: conventional (with pre-winter ploughing), reduced (disk harrow + tillage unit) and zero tillage (no tillage – applica­tion of glyphosate-containing herbicide + direct sowing). The soil was collected with a Van der Horst core sampler into the columns. In a stationary spray chamber, a herbicide containing terbuthylazine was applied at the recommended dose of 500 g/ha. On the basis of the conducted research, it was found that terbuthylazine is a substance with medium persistence in soil (DT50 = 27–34 days). This substance shows the slowest degradation in soil with a zero tillage system. On the other hand, the lack of disturbing the soil structure has a positive effect on the mobility of the herbicide, allowing it to move faster outside the plant root zone.

Biological and economic efficiency of glyphosate-containing herbicide mixtures for pre-plant burndown control of Conyza spp.

Biological and economic efficiency of glyphosate-containing herbicide mixtures for pre-plant burndown control of Conyza spp.

Risk Assessment of Glyphosate Exposures from Pilot Study with Simulated Heavy Residential Consumer Application of Roundup® using a Margin of Safety (MOS) Approach.

Due to the widespread application of glyphosate, a nonselective herbicide, to a variety of resistant food crops, the general population is exposed to glyphosate through dietary intake. Despite this, dietary exposures to glyphosate are considered low in comparison to application-related exposures. Although previous studies have evaluated exposure to horticultural and agricultural workers, to date only one study, which we recently conducted, has characterized exposure to glyphosate in consumers following heavy residential application of a glyphosate-containing herbicide in a residential yard and garden setting. In this previous study, we demonstrated that urinary glyphosate concentrations in these applicators were similar to or in some circumstances greater than those in occupational applicators, likely due to the nature of the simulation study, which ensured a heavy application protocol. However, it is unknown whether these urinary glyphosate concentrations in consumer applicators correspond to internal doses that may be of concern. Therefore, the purpose of this study is to provide a comprehensive risk assessment of glyphosate exposure in consumer applicators using a margin of safety approach. Here, we incorporated data collected from multiple spot urine samples across time from our previous study that assessed consumer exposure to glyphosate from Roundup® application. Estimated internal doses, even with the use of conservative assumptions across unique approaches, were below internal doses estimated from established health-based guidance values. Overall, this study demonstrates that glyphosate exposure from even heavy consumer application of a commercially available glyphosate-containing herbicide does not appear to be a health concern.

Selection for Resistance to a Glyphosate-Containing Herbicide in Salmonella enterica Does Not Result in a Sustained Activation of the Tolerance Response or Increased Cross-Tolerance and Cross-Resistance to Clinically Important Antibiotics

Evolution of bacterial tolerance to antimicrobials precedes evolution of resistance and may result in cross-tolerance, cross-resistance, or collateral sensitivity to other antibiotics. Transient exposure of gut bacteria to glyphosate, the world's most widely used herbicide, has been linked to the activation of the stress response and changes in susceptibility to antibiotics. In this study, we investigated whether chronic exposure to a glyphosate-based herbicide (GBH) results in resistance, a constitutive activation of the tolerance and stress responses, and cross-tolerance or cross-resistance to antibiotics. Of the 10 farm animal-derived clinical isolates of Salmonella enterica subjected to experimental evolution in increasing concentrations of GBH, three isolates showed stable resistance with mutations associated with the glyphosate target gene aroA and no fitness costs. Global quantitative proteomics analysis demonstrated activation of the cellular tolerance and stress response during the transient exposure to GBH but not constitutively in the resistant mutants. Resistant mutants displayed no cross-resistance or cross-tolerance to antibiotics. These results suggest that while transient exposure to GBH triggers cellular tolerance response in Salmonella enterica, this response does not become genetically fixed after selection for resistance to GBH and does not result in increased cross-tolerance or cross-resistance to clinically important antibiotics under our experimental conditions.IMPORTANCE Glyphosate-based herbicides (GBH) are among the world's most popular, with traces commonly found in food, feed, and the environment. Such high ubiquity means that the herbicide may come into contact with various microorganisms, on which it acts as an antimicrobial, and it may select for resistance and cross-resistance to clinically important antibiotics. It is therefore important to estimate whether the widespread use of pesticides may be an underappreciated source of antibiotic-resistant microorganisms that may compromise efficiency of antibiotic treatments in humans and animals.

Pilot study evaluating inhalation and dermal glyphosate exposure resulting from simulated heavy residential consumer application of Roundup®

Objectives The purpose of this study was to evaluate the individual contributions of inhalation and dermal exposures to urinary glyphosate levels following the heavy residential consumer application of a glyphosate-containing herbicide. Methods A pilot study was conducted in which each participant mixed and continuously spray-applied 16.3 gallons of a 0.96% glyphosate-containing solution for 100 min using a backpack sprayer. Twelve participants were divided evenly into two exposure groups, one equipped to assess dermal exposure and the other, inhalation exposure. Personal air samples (n = 12) and dermal patch samples (n = 24) were collected on the inhalation group participants and analyzed for glyphosate using HPLC-UV. Serial urine samples collected 30-min prior to application and 3-, 6-, 12-, 24-hr (inhalation and dermal groups) and 36-hr (dermal group only) post-application were analyzed for glyphosate and glyphosate’s primary metabolite (AMPA) using HPLC-MS/MS. Results The mean airborne glyphosate concentration was 0.0047 mg/m3, and the mean concentrations of glyphosate for each applicator’s four patch samples ranged from 0.04 µg/mm2 to 0.25 µg/mm2. In general, urinary glyphosate, AMPA, and total effective glyphosate levels were higher in the dermal exposure group than the inhalation exposure group, peaked within 6-hr following application, and were statistically indistinguishable from background at 24-hr post-application. Conclusions This is the first study to characterize the absorption and biological fate of glyphosate in residential consumer applicators following heavy application. The results of this pilot study are consistent with previous studies that have shown that glyphosate is rapidly eliminated from the body, typically within 24 hr following application.

Herbicide programs for the termination of grass and broadleaf cover crop species

AbstractThe use of cover crops in soybean production systems has increased in recent years. There are many questions surrounding cover crops—specifically about benefits to crop production and most effective herbicides for spring termination. No studies evaluating cover crop termination have been conducted across a wide geographic area, to our knowledge. Therefore, field experiments were conducted in 2016 and 2017 in Arkansas, Indiana, Mississippi, Missouri, and Wisconsin for spring termination of regionally specific cover crops. Glyphosate-, glufosinate-, and paraquat-containing treatments were applied between April 15 and April 29 in 2016 and April 10 and April 20 in 2017. Visible control of cover crops was determined 28 days after treatment. Glyphosate-containing herbicide treatments were more effective than paraquat- and glufosinate-containing treatments, providing 71% to 97% control across all site years. Specifically, glyphosate at 1.12 kg ha−1 applied alone or with 2,4-D at 0.56 kg ha−1, saflufenacil at 0.025 kg ha−1, or clethodim at 0.56 kg ha−1 provided the most effective control on all grass cover crop species. Glyphosate-, paraquat-, or glufosinate-containing treatments were generally most effective on broadleaf cover crop species when applied with 2,4-D or dicamba. Results from this research indicate that proper herbicide selection is crucial to successfully terminate cover crops in the spring.

Minimum Inhibitory Concentration of Glyphosate and a Glyphosate-Containing Herbicide in Salmonella enterica Isolates Originating from Different Time Periods, Hosts, and Serovars.

Glyphosate, the active compound of Roundup, is one of the most used pesticides in the world. Its residues are often detected in animal feed, but the impact on the animal gut microbiota and on pathogens of the intestine has not intensively been investigated. In this study, we analyzed the minimum inhibitory concentration (MIC) of glyphosate isopropylamine salt and a common glyphosate-containing herbicide formulation in 225 Salmonella enterica isolates by broth microdilution. A bacteriostatic effect of glyphosate on Salmonella growth was detected at the concentration range of 10 to 80 mg/mL for both the active ingredient and the ready-to-use formulation. Time/year of isolation, host species, and serovars revealed a statistically significant influence on MIC values. Recently collected Salmonella isolates had significantly higher MIC values for glyphosate and the glyphosate-containing product compared with isolates collected between 1981 and 1990. Isolates from pigs showed significantly higher MIC values compared with isolates from poultry, and isolates of the Salmonella serovar Typhimurium had significantly higher MIC values than Salmonella Enteritidis and Infantis isolates.

Minimum Inhibitory Concentration of Glyphosate and of a Glyphosate-Containing Herbicide Formulation for Escherichia coli Isolates - Differences Between Pathogenicand Non-pathogenic Isolates and Between Host Species.

Glyphosate is the most extensively used herbicide in the world. However, concerns regarding its safety, side effects, and impact on other organisms have increased in recent years. This is the first study to analyze a large set of recent and historical Escherichia coli isolates varying in pathogenicity and beta-lactam resistance from different host species for their susceptibility to glyphosate isopropylamine salt (IPA), the active ingredient of the herbicide, and to a complete glyphosate-containing formulation (Roundup LB Plus). For this, minimum inhibitory concentrations (MIC) were determined for 238 E. coli isolates by broth microdilution in Mueller Hinton I media followed by the statistical analyses using Mann-Whitney-U test, multivariable analysis of variance (ANOVA) and a multivariable proportional-odds ordinal regression model. While the overall MIC distribution was narrow and lacked a highly resistant sub-population for both substances, statistical analyses revealed small but significant associations between glyphosate resistance levels and different factors tested. Mean MIC values for the entire dataset showed a higher level of resistance to the complete glyphosate-containing formulation (40 mg/ml IPA) than to pure glyphosate (10 mg/ml IPA) in E. coli. Isolates that originated from poultry had significantly higher MIC values for both pure glyphosate and the complete formulation. Pathogenic and non-extended-spectrum beta-lactamase (non-ESBL) E. coli isolates each showed significantly higher MIC values compared to commensals and ESBL-producing E. coli in pure glyphosate, but not in the complete formulation. Recently sampled isolates showed statistically higher MICs than the isolates of the historic standard E. coli collection of reference in pure glyphosate, when tested by nonparametric Mann-Whitney-U test, but not in the multivariable model. Further investigations are necessary to confirm whether these associations have a casual relationship with the glyphosate use or are the consequence of co-selection due to the increased application rates of antibiotics, heavy metals or other biocides. A possible accumulation of pathogenic bacteria in livestock animals fed with glyphosate-containing feed should also be considered.

Acute exposure to a glyphosate-containing herbicide formulation inhibits Complex II and increases hydrogen peroxide in the model organism Caenorhabditis elegans

Glyphosate-based herbicides, such as Touchdown (TD) and Roundup, are among the most heavily-used herbicides in the world. While the active ingredient is generally considered non-toxic, the toxicity resulting from exposure to commercially-sold formulations is less clear. In many cases, cell cultures or various model organisms exposed to glyphosate formulations show toxicity and, in some cases, lethality. Using Caenorhabditis elegans, we assessed potential toxic mechanisms through which a highly-concentrated commercial formulation of TD promotes neurodegeneration. Following a 30-min treatment, we assayed mitochondrial electron transport chain function and reactive oxygen species (ROS) production. Initial oxygen consumption studies indicated general mitochondrial inhibition compared to controls (*p < 0.05). When Complex II activity was further assessed, inhibition was observed in all TD-treated groups (*p < 0.05). Complex IV activity, however, was not adversely affected by TD. This electron transport chain inhibition also resulted in reduced ATP levels (*p < 0.05). Furthermore, hydrogen peroxide levels, but not other ROS, were increased (*p < 0.05). Taken together, these data indicate that commercially-available formulations of TD may exert neurotoxicity through Complex II (succinate dehydrogenase) inhibition, decreased ATP levels, and increased hydrogen peroxide production.

Chronic exposure to a glyphosate-containing pesticide leads to mitochondrial dysfunction and increased reactive oxygen species production in Caenorhabditis elegans

Glyphosate-containing herbicides are among the most widely-used in the world. Although glyphosate itself is relatively non-toxic, growing evidence suggests that commercial herbicide formulations may lead to increased oxidative stress and mitochondrial inhibition. In order to assess these mechanisms in vivo, we chronically (24h) exposed Caenorhabditis elegans to various concentrations of the glyphosate-containing herbicide TouchDown (TD). Following TD exposure, we evaluated the function of specific mitochondrial electron transport chain complexes. Initial oxygen consumption studies demonstrated inhibition in mid- and high-TD concentration treatment groups compared to controls. Results from tetramethylrhodamine ethyl ester and ATP assays indicated reductions in the proton gradient and ATP levels, respectively. Additional studies were designed to determine whether TD exposure resulted in increased reactive oxygen species (ROS) production. Data from hydrogen peroxide, but not superoxide or hydroxyl radical, assays showed statistically significant increases in this specific ROS. Taken together, these data indicate that exposure of Caenorhabditis elegans to TD leads to mitochondrial inhibition and hydrogen peroxide production.

Aquatic hazard assessment of MON 0818, a commercial mixture of alkylamine ethoxylates commonly used in glyphosate-containing herbicide formulations. Part 1: Species sensitivity distribution from laboratory acute exposures.

The sensitivity of 15 aquatic species, including primary producers, benthic invertebrates, cladocerans, mollusks, and fish, to MON 0818, a commercial surfactant mixture of polyoxyethylene tallow amines, was evaluated in standard acute (48-96-h) laboratory tests. In addition, the potential for chronic toxicity (8 d) was evaluated with Ceriodaphnia dubia. Exposure concentrations were confirmed. No significant effects on any endpoint were observed in the chronic test. A tier-1 hazard assessment was conducted by comparing species sensitivity distributions based on the generated data, as well as literature data, with 4 exposure scenarios. This assessment showed moderate levels of hazard (43.1% of the species exposed at or above median effective concentration levels), for a chosen worst-case scenario-unintentional direct over-spray of a 15-cm-deep body of water with the maximum label application rate for the studied formulations (Roundup Original, Vision Forestry Herbicide; 12 L formulation ha-1 , equivalent to 4.27 kg acid equivalent [a.e.] ha-1 ). The hazard decreased to impairment of 20.9% of species under the maximum application rate for more typical uses (6 L formulation ha-1 , 2.14 kg a.e. ha-1 ), and down to 6.9% for a more frequently employed application rate (2.5 L formulation ha-1 , 0.89 kg a.e. ha-1 ). Finally, the percentage (3.8%) was less than the hazardous concentration for 5% of the species based on concentrations of MON 0818 calculated from maximum measured concentrations of glyphosate in the environment. Environ Toxicol Chem 2017;36:501-511. © 2016 SETAC.

Investigations on the possible impact of a glyphosate-containing herbicide on ruminal metabolism and bacteria invitro by means of the 'Rumen Simulation Technique'.

This study was performed in a well-established invitro model to investigate whether the application of a glyphosate-containing herbicide might affect the bacterial communities and some biochemical parameters in a cow's rumen. The test item was applied in two concentrations (high and low) for 5days. In a second trial, fermentation vessels were inoculated with Clostridium sporogenes before the high dose was applied. Effluents were analysed by biochemical, microbiological and genetic methods. A marginal increase in short-chain fatty acid production and a reduction in NH3 -N were observed. There were minor and rather equivocal changes in the composition of ruminal bacteria but no indications of a shift towards a more frequent abundance of pathogenic Clostridia species. Clostridium sporogenes counts declined consistently. No adverse effects of the herbicide on ruminal metabolism or composition of the bacterial communities could be detected. In particular, there was no evidence of a suspected stimulation of Clostridia growth. Antibiotic activity of glyphosate resulting in microbial imbalances has been postulated. In this exploratory study, however, intraruminal application of concentrations reflecting potential exposure of dairy cows or beef cattle did not exhibit significant effects on bacterial communities in a complex invitro system. The low number of replicates (n=3/dose) may leave some uncertainty.

Exposure of C. elegans eggs to a glyphosate-containing herbicide leads to abnormal neuronal morphology

Recent data demonstrate that chronic exposure of Caenorhabditis elegans (C. elegans) to a high-use glyphosate-containing herbicide, Touchdown (TD), potentially damages the adult nervous system. It is unknown, however, whether unhatched worms exposed to TD during the egg stage show abnormal neurodevelopment post-hatching. Therefore, we investigated whether early treatment with TD leads to aberrant neuronal or neurite development in C. elegans. Studies were completed in three different worm strains with green fluorescent protein (GFP)-tagged neurons to facilitate visual neuronal assessment. Initially, eggs from C. elegans with all neurons tagged with GFP were chronically exposed to TD. Visual inspection suggested decreased neurite projections associated with ventral nerve cord neurons. Data analysis showed a statistically significant decrease in overall green pixel numbers at the fourth larval (L4) stage (*p<0.05). We further investigated whether specific neuronal populations were preferentially vulnerable to TD by treating eggs from worms that had all dopaminergic (DAergic) or γ-aminobutyric acid (GABAergic) neurons tagged with GFP. As before, green pixel number associated with these discrete neuronal populations was analyzed at multiple larval stages. Data analysis indicated statistically significant decreases in pixel number associated with DAergic, but not GABAergic, neurons (***p<0.001) at all larval stages. Finally, statistically significant decreases (at the first larval stage, L1) or increases (at the fourth larval stage, L4) in superoxide levels, a developmental signaling molecule, were detected (*p<0.05). These data suggest that early exposure to TD may impair neuronal development, perhaps through superoxide perturbation. Since toxic insults during development may late render individuals more vulnerable to neurodegenerative diseases in adulthood, these studies provide some of the first evidence in this model organism that early exposure to TD may adversely affect the developing nervous system.

Exposure to a glyphosate-based herbicide during pregnancy and lactation induces neurobehavioral alterations in rat offspring

The impact of sub-lethal doses of herbicides on human health and the environment is a matter of controversy. Due to the fact that evidence particularly of the effects of glyphosate on the central nervous system of rat offspring by in utero exposure is scarce, the purpose of the present study was to assess the neurobehavioral effects of chronic exposure to a glyphosate-containing herbicide during pregnancy and lactation. To this end, pregnant Wistar rats were exposed through drinking water to 0.2% or 0.4% of a commercial formulation of glyphosate (corresponding to a concentration of 0.65 or 1.30g/L of glyphosate, respectively) during pregnancy and lactation and neurobehavioral alterations in offspring were analyzed. The postnatal day on which each pup acquired neonatal reflexes (righting, cliff aversion and negative geotaxis) and that on which eyes and auditory canals were fully opened were recorded for the assessment of sensorimotor development. Locomotor activity and anxiety levels were monitored via open field test and plus maze test, respectively, in 45- and 90-day-old offspring. Pups exposed to a glyphosate-based herbicide showed early onset of cliff aversion reflex and early auditory canal opening. A decrease in locomotor activity and in anxiety levels was also observed in the groups exposed to a glyphosate-containing herbicide. Findings from the present study reveal that early exposure to a glyphosate-based herbicide affects the central nervous system in rat offspring probably by altering mechanisms or neurotransmitter systems that regulate locomotor activity and anxiety.

Potential microbial toxicity and non-target impact of different concentrations of glyphosate-containing herbicide (GCH) in a model pervious paving system

Pervious Pavement Systems are Sustainable Drainage devices that meet the three-fold SUDS functions of stormwater quantity reduction, quality improvement and amenity benefits. This paper reports on a study to determine the impact of different concentrations of glyphosate-containing herbicides on non-target microorganisms and on the pollutant retention performance of PPS. The experiment was conducted using 0.0484m2 test rigs based on a four-layered design. Previous studies have shown that PPS can trap up to 98.7% of applied hydrocarbons, but results of this study show that application of glyphosate-containing herbicides affected this capability as 15%, 9% and 5% of added hydrocarbons were released by high (7200mgL−1), medium (720mgL−1) and low (72mgL−1) glyphosate-containing herbicides concentrations respectively. The concentrations of nutrients released also indicate a potential for eutrophication if these effluents were to infiltrate into aquifers or be released into surface waters. The effect of glyphosate-containing herbicides application on the bacterial and fungal communities was slightly different; fungi exhibited a “top-down” trend as doses of 7200mgL−1 glyphosate-containing herbicides yielded the highest fungal growth whilst those with a concentration of 720mgL−1 glyphosate-containing herbicides applied yielded the highest bacterial growth. In the case of protists, doses of glyphosate-containing herbicides above 72mgL−1 were fatal, but they survived at the lower concentration, especially the ciliates Colpoda cucullus and Colpoda steinii thus indicating potential for their use as biomarkers of herbicide-polluted environments. Data also showed that at the lowest concentration of glyphosate-containing herbicides (72mgL−1), biodegradation processes may not be affected as all trophic levels required for optimum biodegradation of contaminants were present.

Hemodialysis clearance of glyphosate following a life-threatening ingestion of glyphosate-surfactant herbicide

Context. Ingestion of glyphosate-surfactant herbicides (GlySH) can result in acute kidney injury, electrolyte abnormalities, acidosis, cardiovascular collapse, and death. In severe toxicity, the use of hemodialysis is reported, but largely unsupported by kinetic analysis. We report the dialysis clearance of glyphosate following a suicidal ingestion of a glyphosate-containing herbicide. Case details. A 62-year-old man was brought to the emergency department (ED) 8.5 h after drinking a bottle of commercial herbicide containing a 41% solution of glyphosate isopropylamine, in polyoxyethyleneamine (POEA) surfactant and water. He was bradycardic and obtunded with respiratory depression necessitating intubation and mechanical ventilation. Initial laboratory results were significant for the following: pH, 7.11; PCO2, 64 mmHg; PO2, 48 mmHg; potassium, 7.8 mEq/L; Cr 3.3, mg/dL; bicarbonate, 22 mEq/L; anion gap, 18 mEq/L; and lactate, 7.5 mmol/L. Acidosis and hyperkalemia persisted despite ventilation and fluid resuscitation. The patient underwent hemodialysis 16 h post ingestion, after which he demonstrated resolution of acidosis and hyperkalemia, and improvement in clinical status. Serum glyphosate concentrations were drawn prior to, during, and after hemodialysis. The extraction ratio and hemodialysis clearance were calculated to be 91.8% and 97.5 mL/min, respectively. Discussion. We demonstrate the successful clearance of glyphosate using hemodialysis, with corresponding clinical improvement in a patient with several poor prognostic factors (advanced age, large volume ingested, and impaired consciousness). The effects of hemodialysis on the surfactant compound are unknown. Hemodialysis can be considered when severe acidosis and acute kidney injury complicate ingestion of glyphosate-containing products.

Utilization of Glyphosate‐Containing Herbicides on Pervious Paving Systems: Laboratory‐Based Experiments to Determine Impacts on Effluent Water Quality

Pervious paving (PPS) model rigs have been used in laboratory‐based experiments to determine the impact of a glyphosate‐containing herbicide (GCH) on the ability of the PPS to retain and biodegrade pollutants. Using three concentrations of GCH (7200, 720, and 72 mg L−1) and used engine oil, it was found that hydrocarbon in the effluent increased with time, and with increasing GCH concentration. Turbidity increased and pH decreased with time, with the highest application of GCH declining by a whole pH unit to become slightly acidic. For Zn, Cu, and Pb, values for the lowest concentration of GCH, whilst slightly higher than the control mirrored its temporal trend and the highest concentration of GCH decreased with time. For the medium GCH concentration, however, Zn and Pb increased toward the end of the monitoring period. Dissolution experiments appeared to confirm speculation that the surfactant in the GCH may be forming an emulsion with the oil facilitating the release of metals associated with the oil. Particulate‐associated pollutants possibly released from the aggregate may be desorbed as the pH declined in the PPS rig. This raises concerns for receiving water quality, particularly with UK legislation encouraging the use of sustainable drainage systems and therefore PPS.