Glycosylated Serum Protein Levels Research Articles

Combined exposure to lead and microplastics increased risk of glucose metabolism in mice via the Nrf2/NF-κB pathway.

The purpose of this study was to explore the effects of combined lead (Pb) and two types of microplastic (MP) (polyvinyl chloride [PVC] and polyethylene [PE]) exposure on glucose metabolism and investigate the role of the nuclear factor erythroid 2-related factor 2 (Nrf2)/nuclear factor-kappa B (NF-κB) signaling pathway in mediating these effects in mice. Adult C57BL/6J mice were randomly divided into four groups: control, Pb (100 mg/L), MPs (containing 10 mg/L PE and PVC), and Pb + MPs, each of which was treated with drinking water. Treatments were conducted for 6 weeks. Co-exposure to Pb + MPs exhibited increase glycosylated serum protein levels, insulin resistance, and damaged glucose tolerance compared with the control mice. Additionally, treatment with Pb + MPs caused more severe damage to hepatocytes than when exposed to them alone concomitantly, exposed to Pb + MPs exhibited improved the levels of interleukin-6, tumor necrosis factor-alpha, and malondialdehyde, but reduced superoxide dismutase, glutathione peroxidase, and catalase assay in livers. Furthermore, they increase the Kelch-like ECH-associated protein 1 (Keap1) and phosphorylated p-NF-κB protein levels but reduced the protein levels of heme oxygenase-1 and Nrf2, as well as increased Keap1 mRNA and Nrf2 mRNA. Co-exposure to Pb + MP impacts glucose metabolism via the Nrf2 /NF-κB pathway.

Therapeutic Potential of Enzymatically Extracted Eumelanin from Squid Ink in Type 2 Diabetes Mellitus (T2DM) ICR Mice: Multifaceted Intervention against Hyperglycemia, Oxidative Stress, and Depression.

Type 2 diabetes mellitus (T2DM) is a prevalent metabolic disease that poses significant health risks due to its numerous complications. However, the effects of eumelanin on oxidative stress, hyperglycemia, and depression in diabetic mice haven't been extensively studied. Our study employed an enzymatic approach to extract eumelanin from squid ink and characterized it using spectroscopic techniques. Remarkably, eumelanin extracted with alkaline-neutral-flavor protease (ANF) displayed superior inhibitory activity against α-glucosidase and α-amylase, while enhancing glucose utilization and hepatic glycogen synthesis in human hepatocellular carcinoma cell line (HepG2) insulin resistance model. Further evaluation of ANF in a T2DM ICR mouse model demonstrated its significant potential in alleviating hyperglycemia, reducing glycosylated serum protein levels, improving glucose tolerance, and modulating total cholesterol (TC), low-density lipoprotein (LDL) levels, as well as antioxidant indices (SOD, GSH-Px, and MDA) at a dosage of 0.04 g·kg-1 . Additionally, ANF exhibited positive effects on energy levels and reduced immobility time in antidepressant behavioral experiments. Moreover, ANF positively influenced the density and infiltration state of renal cells, while mitigating inflammatory enlargement and deformation of liver cells, without inducing any adverse effects in mice. Overall, these findings underscore the significant therapeutic potential of ANF in the treatment of T2DM and its associated complications. By augmenting lipid and glucose metabolism, mitigating oxidative stress, and alleviating depression, ANF emerges as a promising candidate for multifaceted intervention. This article is protected by copyright. All rights reserved.

Rapid estimation approach for glycosylated serum protein of human serum based on the combination of deep learning and TD-NMR technology

Rapid and precise estimation of glycosylated serum protein (GSP) of human serum is of great importance for the treatment and diagnosis of diabetes mellitus. In this study, we propose a novel method for estimation of GSP level based on the combination of deep learning and time domain nuclear magnetic resonance (TD-NMR) transverse relaxation signal of human serum. Specifically, a principal component analysis (PCA)-enhanced one-dimensional convolutional neural network (1D-CNN) is proposed to analyze the TD-NMR transverse relaxation signal of human serum. The proposed algorithm is proved by accurate estimation of GSP level for the collected serum samples. Furthermore, the proposed algorithm is compared with 1D-CNN without PCA, long short-term memory network (LSTM) and some conventional machine learning algorithms. The results indicate that PCA-enhanced 1D-CNN (PC-1D-CNN) has the minimum error. This study proves that proposed method is feasible and superior to estimate GSP level of human serum using TD-NMR transverse relaxation signals.

Berberine ameliorates rats model of combined Alzheimer’s disease and type 2 diabetes mellitus via the suppression of endoplasmic reticulum stress

This study is aimed to investigate the protective effect against type 2 diabetes mellitus (T2DM) and Alzheimer's disease (AD) of Berberine (BBR), and the underlying mechanism of action is explored. We established a rat model of combined AD and T2DM and used it to investigate the effect of BBR (150mg/kg) on the course of these pathologies. The Morris water maze, biochemical analysis, hematoxylin-eosin staining, immunohistochemical study, immunofluorescent staining, TUNEL assay, RT-qPCR and western blot were used to reveal the effect of BBR on blood glucose, lipid changes, hippocampal injuries and cognitive impairment. The results showed that BBR could alleviate memory deficits, restore the disordered arrangement of nerve cells, the damage of neurons, improve TUNEL-positive cells and decrease the elevated levels of fasting blood glucose, triglyceride, total cholesterol and glycosylated serum protein levels in Alzheimer diabetic rats. Moreover, BBR treatment reduces the transcription of mRNAs and expression of proteins related to endoplasmic reticulum (ER) stress. These findings conclude that BBR can protect neurons by inhibiting the pathway of ER stress and thereby play an essential role in the preventive and therapeutic of AD and T2DM.

A comparison between whole mung bean and decorticated mung bean: beneficial effects on the regulation of serum glucose and lipid disorders and the gut microbiota in high-fat diet and streptozotocin-induced prediabetic mice.

The aim of this study is to investigate the beneficial effects of whole mung bean (WMB) and decorticated mung bean (DMB) on the regulation of serum glucose and lipid disorders in high-fat diet (HFD) and streptozotocin (STZ)-induced prediabetic mice, and to further explore their gut microbiota modulatory effects. In the present study, the ability of mung bean-based diets to combat prediabetes-related metabolic disorders was determined by assessing the changes in the physiological, biochemical, and histological parameters, and the gut microbiota composition of prediabetic mice. The supplementation of both WMB and DMB can effectively alleviate HFD and STZ-induced impaired glucose tolerance (P < 0.05), which was accompanied by improvements in pancreatic β-cell damage and hepatic steatosis. However, only WMB supplementation significantly decreased the fasting blood glucose and fasting serum insulin levels by sensitizing insulin action (P < 0.05), and reduced the serum lipid profiles and glycosylated serum protein levels (P < 0.05). Furthermore, high-throughput pyrosequencing of the 16S rRNA gene revealed that WMB and DMB supplementation could prevent HFD and STZ-induced gut microbiota dysbiosis, especially for the enrichment of some benign bacteria, such as Bifidobacterium and Akkermansia, and the reduction of some harmful bacteria (Staphylococcus and Enterococcus). Overall, although decortication processing had an impact on the beneficial effects of mung bean, it did not cause the loss of all health benefits.

Silk sericin has significantly hypoglycaemic effect in type 2 diabetic mice via anti-oxidation and anti-inflammation

The sericin protein from silk-processing waste added to the normal diet at 0.8% (g%) level was administered orally to type 2 diabetic (T2D) mice to investigate its hypoglycaemic effects and mechanism. The oral protein is in the form of silk sericin hydrolysate, obtained from a boiling treatment of 0.025% calcium hydroxide solution. The protein significantly decreased fasting blood glucose, fasting plasma insulin, and glycosylated serum protein levels; improved oral glucose tolerance and insulin tolerance, and enhanced antioxidative activities. The protein could ameliorate the pathological damage in pancreatic β-cells and the liver tissue. It enhanced the expression of key proteins and enzymes, including insulin receptor, insulin receptor substrate, PI3K, phosphorylated-AKT, hepatic kinase, GLUT4, glycogen synthase, GSK3β, GLK, PFK1, PKM2, and AMPKα, which are related to insulin metabolism and glycolysis. The protein also reduced the expression of G6Pase, PCK, and ACC, which are related to gluconeogenesis and lipid metabolism in the liver, and decreased the expression of TNF-α, IL-6, P65, and IKKβ related to inflammation. In general, sericin could maintain normal glucose levels and regulate insulin secretion, insulin and lipid metabolism, and inhibition of inflammation. Therefore, sericin protein could be developed into a novel functional health food with significantly hypoglycaemic effect.

Glucose metabolic effects of oat noodles with different processing in type 2 diabetic mice

Oat noodles are becoming popular for its health and flavor. The study investigated the glucose metabolic effects of oat noodles with different processing in streptozotocin-induced diabetic mice. Oat noodles were prepared by traditional method (TON), extrusion (EON) and extrusion-freezing (FON), respectively, and the glucose metabolic effects were also investigated with C57BL/6 male mice. It was found that all three oat noodles types significantly reduced the fasting blood glucose and glycosylated serum protein level (p < 0.05), as well as improved glucose tolerance ability (p < 0.05). All types of oat noodles effectively (p < 0.05) increased fasting insulin and glycogen metabolism level (p < 0.05), inhibited intestinal enzyme activity as well as improved the serum lipids and liver function regulation (p < 0.05). TON and FON showed excellent ability on reducing fasting blood glucose and glycosylated serum protein than EON, while FON with significantly lower low-density lipoprotein cholesterol and aspartate aminotransferase might be better than TON. Our results suggested that all types of oat noodles, especially TON and FON had hypoglycemic and hypocholesterolemic effects on diabetic mice and was potential to be an alternative dietary intervention for diabetic adjustment.

Gynura divaricata rich in 3, 5\u2212/4, 5-dicaffeoylquinic acid and chlorogenic acid reduces islet cell apoptosis and improves pancreatic function in type 2 diabetic mice

BackgroundDiabetes mellitus is one of the most common chronic diseases that accompanied by severe complications. Gynura divaricata (GD), a medicinal and edible plant that is usually used for the treatment of diabetes. Therefore, this study investigates the chemical components of GD with hypoglycemic effect and the possible mechanism lowering blood sugar in T2D diabetic mice.MethodsThe methanol extract of GD was analysed by HPLC-DAD. And then mice with type 2 diabetes induced by a high-fat diet in combination with streptozotocin feed the diet containing lyophilized GD powder for 4 weeks. During this period, fasting blood glucose (FBG) levels and body weight were measured.ResultsGD was rich in four bioactive components of dicaffeoylquinic acid and chlorogenic acid. These components occupied about 2.37% in the GD powder in which the highest level was 3, 5-dicaffeoylquinic acid. Oral GD significantly reduced FBG, fasting serum insulin, and glycosylated serum protein levels, and enhanced antioxidative activities. HE-staining showed that the pathological damage in pancreatic β-cells was ameliorated. An immunohistochemical assay also showed that GD promoted marked pancreatic β-cell regeneration. GD also caused notable increase in GLUT2, GK, MafA, PDX-1, and Bcl-2 as well as reduction in Bax and caspase-3 expression as shown by western blot analysis.ConclusionsGD exerts the pronounced hypoglycaemic effect by inhibiting islet cell apoptosis and improving pancreatic function. Therefore, GD might have a potential to improve diabetes.

Dendrobium mixture regulates hepatic gluconeogenesis in diabetic rats via the phosphoinositide-3-kinase/protein kinase B signaling pathway.

The present study aimed to evaluate the impact of dendrobium mixture (DMix) on the gene and protein expression of insulin signaling pathway-associated factors in the livers of diabetic rats. The molecular mechanisms by which DMix inhibits gluconeogenesis were also investigated. A total of 47 female Wistar rats were used in the present study. Of these, 11 rats were randomly selected as healthy controls and diabetes was induced in the remaining 36 rats by administering a high-fat and high-sugar diet for 6 weeks, followed by two intraperitoneal injections of streptozotocin. The 36 rats were screened for diabetes and then randomly divided into three groups: Model, metformin and DMix groups. Following 12 weeks of treatment, the fasting blood glucose (FBG), glycosylated serum protein (GSP), serum insulin, blood lipids [total cholesterol (Tch) and triglycerides (TG)], alanine transaminase (ALT) and aspartate transaminase (AST) were assessed. In addition, hematoxylin and eosin staining was used for histomorphological examination of the liver tissues. The mRNA expression of insulin receptor (InsR), forkhead box protein O1 (FoxO1), phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6-phosphatase (G6Pase) in the liver was measured with reverse transcription-quantitative polymerase chain reaction and the protein expression of InsR, phosphoinositide-3-kinase (PI3K), phosphorylated (p)-PI3K, protein kinase B (Akt), p-Akt, FoxO1, PEPCK and G6Pase in the liver was measured by western blot analysis. The FBG, GSP, InsR, Tch, TG, ALT and AST levels were significantly lower in the DMix-treated group compared with the model group (P<0.05). In addition, DMix treatment notably improved liver histopathology and significantly increased the gene and protein expression of InsR, PI3K and Akt (P<0.05). DMix treatment also significantly reduced the gene and protein expression of FoxO1, PEPCK and G6Pase (P<0.05). DMix effectively reduced FBG and blood lipids and significantly improved liver function and insulin resistance in diabetic rats, possibly by regulating the gene and protein expression of molecules associated with the PI3K/Akt signaling pathway.

Effects of Polygonum Cuspidatum Total Anthraquinone on Diabetic Mice Model

objective: effects of Polygonum cuspidatum total anthraquinone on streptozotocin induced diabetic mouse model, To investigate the hypoglycemic mechanism and characteristics of Polygonum cuspidatum anthraquinone. Method: By intravenous injecting streptozotocin induced diabetic mice model, detecting the serum glucose, glycosylated serum protein content, observing the pancreas pathological changes, Studying Polygonum cuspidatum on blood glucose in the mice pancreas. Result：Successful established diabetic mice model. Compared with model group, The metformin group, high, medium and low doses Polygonum cuspidatum total anthraquinone can reduce the blood sugar and glycosylated serum protein level in diabetic model mice at different levels(P<0.01, P<0.05), and increased liver glycogen, To inhibit the atrophy, necrosis, dissolution and degeneration of pancreatic islet cells in mice. Conclusion: Polygonum cuspidatum total anthraquinone has the effects of reducing blood sugar, glycosylated serum protein level and reducing blood fat, Promoting insulin secretion and hepatic glycogen formation, improve insulin resistance, Improving the pathological changes of pancreas in diabetic mice, It has a good hypoglycemic effect, delayed the process of diabetes to some extent.

Effects of Qizhi Jiangtang capsule on protein expressions of InsR, PI3K, GLUT2 and p-JNK in hepatic tissues of rats with type 2 diabetes

To observe the hypoglycemic effect of Qizhi Jiangtang capsule in rats with type 2 diabetes, and investigate the preliminary mechanism of its hypoglycemic effect, type 2 diabetes rat models were established by high glucose and high fat combined with small dose of streptozotocin (STZ). After continuous administration for 6 weeks, blood glucose, and glycosylated serum protein (GSP) levels were detected in all of the animals; immunohistochemistry assay was used to detect the number of islet β cells; Western blot assay was used to detect the protein expression levels of insulin receptor (InsR), phosphoinositide-3 kinases (PI3K), glucose transporter-2 (GLUT2) and phosphorylated Jun N-terminal kinases (p-JNK)in hepatic tissues. The results showed that Qizhi Jiangtang capsule could reduce the blood sugar and GSP levels in serum in animals with type 2 diabetes mellitus, increase the level of insulin in serum and number of islet β cells, increase the protein expression levels of InsR, PI3K and GLUT2, and reduce the level of p-JNK protein expression. In conclusion, Qizhi Jiangtang capsule has relatively stable hypoglycemic effect, and the mechanism may be associated with increasing the number of islet β cells and level of insulin in serum, up-regulating the protein expression levels of InsR, PI3K and GLUT2, down-regulating the level of p-JNK protein expression in hepatic tissues, and reducing the level of insulin in hepatic tissues.

Antidiabetic potential of phycocyanin: Effects on KKAy mice

Context: Phycocyanin (PC) has been proven to have many therapeutic properties, but its effects on diabetes have not been investigated.Objective: Antidiabetic activity of PC isolated from Spirulina platensis was evaluated in this study.Materials and methods: Oral administration of PC (100 mg/kg, once per day for 3 weeks) on KKAy mice were investigated by monitoring the changes in body weight, food intake, fasting plasma glucose level, 24 h random blood glucose levels, oral glucose tolerance tests (OGTTs), glycosylated serum protein (GSP), fasting serum insulin (FINS), glycogen, triglyceride (TG), total cholesterol (TC), total antioxidative capability (T-AOC) and malondialdehyde (MDA). Histopathological changes in the pancreas were also examined with hematoxylin-eosin staining.Results: Administration of PC significantly decreased the body weight, fasting plasma glucose, 24 h random blood glucose levels, FINS and GSP levels, TG and TC content in serum and livers, MDA content in livers (p < 0.05 or p < 0.01). On the other hand, glucose tolerance to glucose administration, T-AOC, and the content of glycogen in liver and muscle were enhanced following PC treatment (p < 0.05 or p < 0.01). Histopathological results showed that PC administration suppressed the abnormal enlargement of islets observed in the pancreas of KKAy mice.Discussion and conclusion: The antidiabetic effect of PC on KKAy mice is most likely due to its ability to enhance insulin sensitivity, amelioration of insulin resistance of peripheral target tissues and regulation of glucolipide metabolism. Therefore, PC may have a potential clinical utility in combating type-2 diabetes.

Glucose and lipid homeostasis in adult rat is impaired by early-life exposure to perfluorooctane sulfonate

Perfluorooctane sulfonate (PFOS), which belongs to the degradation product of many perfluorinated compounds, is on the list of persistent organic pollutants (POPs) and is currently detected in both wildlife and humans. The consequence of gestational and lactational exposure to PFOS on prediabetes effect in offspring was investigated in rats in the present study. Maternal rats were treated with vehicle, 0.5 mg/kg/day or 1.5 mg/kg/day PFOS respectively from gestation day 0 to postnatal day 21. The glucose and lipid metabolism effects were investigated on the offspring in adulthood. The gestational and lactational exposure to PFOS led to low body weight from birth to weaning, and evoked signs of a prediabetic state, with elevated fasting serum insulin and leptin level, impaired glucose tolerance, though the fasting serum glucose and glycosylated serum protein level were normal. Abnormal lipid homeostasis was also observed by the phenomenon of hepatic steatosis and increased gonadal fat pad weight. However, the circulating serum level of fasting triglyceride and cholesterol level were no different from controls. Our results suggested that developmental exposure to PFOS may contribute to glucose and lipid metabolic disorder in adulthood.

Glycosylated Serum Protein May Improve Our Ability to Predict Endothelial and Erectile Dysfunction in Nonorganic Patients

Early prediction of erectile dysfunction (ED) is critical in the treatment of impotence. Underlying pathogenesis may be the reason for ED without organic causes in young men. We evaluated the early predictive value of glycosylated serum protein (GSP) in young patients whose ED was diagnosed as "nonorganic" in origin according to general criteria. A total of 150 young men with ED and 27 healthy men without ED were evaluated, including International Index of Erectile Function-5 (IIEF-5), causes of ED, influential or risk factors for ED, vascular parameters, and serum biochemical markers. Fifty-two ED patients aged 20-40 years without known etiology and 22 age-matched normal subjects were enrolled. The further assessment of two groups focused on vascular endothelial function and glycometabolic state. Relationships among the IIEF-5 scores, flow-mediated dilation (FMD), and GSP were analyzed in cases vs. controls, using Pearson's correlation and multiple linear regression analysis. No significant differences in baseline characteristics, cardiovascular risks, and conventional biomarkers were found between testing and control groups, except fasting blood glucose level (4.69 ± 0.50 vs. 4.29 ± 0.48, P = 0.003). FMD values were significantly reduced in cases compared with controls and correlated positively with IIEF-5 scores (r = 0.629, P < 0.001). GSP levels were significantly increased in the ED cases compared with controls and correlated negatively with IIEF-5 scores (r = -0.504, P < 0.001) and FMD values (r = -0.469, P < 0.001). These parameters independently predicted ED presence. The positive predictive value of FMD > 11.55% for excluding ED and of GSP > 210.50 mg/L for diagnosing ED were 86.4% (area under the curve [AUC]: 0.942, specificity: 88.4%) and 84.5% (AUC: 0.864, specificity: 72.7%), respectively. Underlying glycometabolic disorder and subclinical endothelial dysfunction may be served as early markers for organic ED in young ED patients without well-known related risk factors. GSP level may improve our ability to predict endothelial dysfunction and erectile dysfunction.

Effects of Astragalus Polysaccharides on Chymase, Angiotensin-converting Enzyme and Angiotensin II in Diabetic Cardiomyopathy in Hamsters

This study investigated the effects of Astragalus polysaccharides (APS), the main active extract from the traditional Chinese medicinal herb Astragalus membranaceus, on myocardial chymase, angiotensin-converting enzyme (ACE) and angiotensin II (Ang II) in diabetic cardiomyopathic hamsters. Plasma levels of insulin, C-peptide and glycosylated serum protein (GSP), plasma and myocardial levels of Ang II, and myocardial gene expression and activity of chymase and ACE were measured after treatment with APS at a dose of 1 g/kg per day or 1 ml of normal saline per day (controls) for 10 weeks. GSP levels, myocardial Ang II levels, and myocardial gene expression and activity of chymase were significantly decreased in diabetic hamsters after treatment with APS compared with controls. These results suggest that APS can inhibit the local chymase-Ang II system in diabetic cardiomyopathy.

Effects of lithium deficiency in some insulin-sensitive tissues of diabetic Chinese hamsters.

In this work, we report the effect of low-dose lithium carbonate on blood glucose levels and tissue lithium content in hereditary spontaneous diabetic Chinese hamsters (HSDCHs). Hepatic lithium levels are significantly lower in diabetic hamsters when compared to healthy controls: 2.05 +/- 0.26 and 3.04 +/- 0.11 micrograms/g, respectively. The same trend was observed in kidney and muscle: 18.26 +/- 0.24 vs 20.23 +/- 1.10 micrograms/g and 4.66 +/- 0.17 vs 5.95 +/- 0.67 micrograms/g, respectively. The significance level was p < 0.05 in all cases. Supplementation with lithium carbonate eliminated tissue lithium deficiency, and had a normalizing effect on blood glucose and glycosylated serum protein levels. The insulin sensitivity index (ISI) increased, thus reducing insulin resistance. Our results suggest that lithium deficiency in certain insulin-sensitive tissues may be associated with blood glucose imbalance resulting from insulin resistance.

Effect of wheat bran supplementation on blood sugar, glycosylated protein and serum lipids in NIDDM subjects

Wheat bran supplementation (11 gm per day) was carried out for a period of two months in 30 maturity onset diebetics. The efficacy of bran supplementation was monitored by measuring the fasting and 2 h post prandial blood sugar levels, glycosylated serum protein levels, glycosylated albumin levels and serum lipid and lipoprotein cholesterol levels. The results indicated a transient reduction in fasting and 2 h post prandial blood sugar levels after one month's bran therapy. No appreciable changes in the serum glycosylated protein levels, glycosylated albumin levels, serum lipids and lipoprotein cholesterol levels were observed during the period of supplementation.

Brief reports with panel discussion: Il. Glycosylated serum protein level as a screening and diagnostic test for gestational diabetes mellitus

Glycosylated serum protein assay was examined as an alternative to standard glucose screening and glucose tolerance testing. In a comparison of two groups of gravid women having abnormal 1-hour 50 gm glucose screening tests, there was no difference in glycosylated protein level in the group with abnormal glucose tolerance test results (9.4% ± 2.0%, mean ± SD; n = 8) versus normal results (9.2% ± 1.07%, mean ± SD; n = 11). Furthermore, correlation of glycosylated serum protein level with glucose screening test results was poor (r = 0.185, p = 0.23, n = 17). Glycosylated serum protein assay is not useful in detecting mild metabolic aberrations associated with gestational diabetes.

Glycosylated serum protein levels in diabetic and nondiabetic pregnant patients: An indicator of short-term glycemic control in the diabetic patient

Measurement of the level of nonenzymatic glycosylation of blood proteins with more rapid turnover times than hemoglobin has been suggested as an indicator of time-averaged glucose control in nonpregnant diabetic patients. Using affinity chromatography, we have measured the levels of glycosylated serum proteins during pregnancy in 14 normal volunteers and 15 insulin-dependent diabetic patients. No relationship was noted between the percentage of glycosylated serum proteins in serum from normal patients and the gestational age at the time of sampling in the second and third trimesters of pregnancy. When the relative frequency distribution of glycosylated serum protein levels in normal patients was compared with that in diabetic patients, a significant difference was noted between the two groups, with a higher percentage of glycosylated serum protein levels in diabetic patients being at elevated values compared to those in normal patients. Normal patients had measured glycosylated serum protein levels of 12.5% ± 2.2% whereas diabetic patients had glycosylated serum protein levels of 14.0% ± 3.6%. When peak fasting serum glucose and high Chemstrip glucose levels were compared with glycosylated serum proteins in the diabetic population, a significant correlation for each was noted. The best correlation resulted from a comparison of an average Chemstrip glucose level (mean of 49 glucose values during the previous week) and the glycosylated serum protein value obtained at the end of that week. This inexpensive assay can be adapted to any clinical laboratory and should provide an objective means to evaluate short-term glycemic control, complementing the evaluation provided by self-glucose monitoring (immediate control) and intermitten assay of glycosylated hemoglobin (long-term control).

Non-enzymatically glycosylated serum protein in diabetes mellitus: an index of short-term glycaemia.

We measured non-enzymatically-glycosylated serum protein by a colorimetric assay in 107 diabetic and 82 control subjects. The mean level in diabetics was more than twice that in controls. Cross sectional and longitudinal studies in diabetic patients showed that glycosylated serum protein levels correlated with both fasting serum glucose and glycosylated haemoglobin levels. The correlation between glycosylated serum protein and fasting serum glucose was closer in Type 2 than in Type 1 diabetes. Treatment aimed at improving control in eight poorly controlled diabetic patients resulted in a 37% mean fall in glycosylated serum protein within one week, whereas glycosylated haemoglobin decreased only 8%. These studies confirm that non-enzymatic glycosylation of serum proteins is enhanced in diabetes. Measurement of glycosylated serum protein appears to provide an index of glycaemia over the preceding several days. It has the advantage of detecting improvements in glycaemic control much sooner than does glycosylated haemoglobin measurement.