Abstract The potato (Solanum tuberosum) is a staple food worldwide, but modern potato cultivation relies heavily on the use of pesticides to control pests and diseases. However, many wild Solanum species are highly resistant to biotic and abiotic stresses relevant for potato production. Several of those species have been used in potato breeding to confer resistances which has only been moderately successful. Instead, we propose an alternative approach to utilize the potential of wild Solanum germplasm. Recently, de novo domestication has been suggested to create more resilient crops: Instead of introducing resistance genes into existing crops, domestication traits could be introduced into resistant wild crop relatives to create new crops. Therefore, we selected ten promising species from the 107 known wild tuber-bearing Solanum species for their resistance to biotic and abiotic stresses based on existing scientific literature for experimental characterization looking at tuberization under short and long-day conditions, tuber glycoalkaloid content, starch digestibility and performance in tissue culture. Based on this, the highly pest and disease resistant S. bulbocastanaum was chosen. Our results showed that it produced relatively large tubers, also under long-day conditions and performed exceptionally well in tissue culture.