Glutamic Oxaloacetic Transminase Research Articles

Studies on the genetic diversity of pointed gourd using biochemical methods (<i>Isozyme analysis</i>)

Biochemical characterizations of 64 pointedgourds were done using three isozyme viz., acid phosphatase, peroxidase and glutamate oxaloacetate transaminase. A wide range of diversity among the gremplasm based on their acid phosphatase, peroxidase and glutamate oxaloacetate transaminase isoenzyme banding patterns were observed. In respect of isoenzyme activity; 8 acid phosphatase, 7 peroxidase and 9 glutamate oxaloacetate transminase electrophoretic zymotypes were formed by 19, 11, and 19 bands at different Rf values varying from 0.19 to 0.82, 0.38 to 0.69 and 0.15 to 0.95, respectively. The wide range of similarity co-efficient of 0.0-80.0, 0.0-66.0, and 0.0-80.0 as found among the electrophoretic patterns in acid phosphatase, peroxidase, and glutamate oxaloacetate transminase, respectively, indicating wide genetic diversity among the accessions. Based on the polymorphic activity of these three enzymes, 27 combinations of electrophoretic zymotypes were identified, each of which can he equated to genotypes. Each of the groups consisted of one to eight genotypes. Sixty four accessions of pointed gourd were grouped into 12 clusters. The genotypes collected from the same location were grouped into different clusters. Key Words: Genetic diversity; pointed gourd; biochemical methods. DOI: 10.3329/bjar.v34i1.5762Bangladesh J. Agril. Res. 34(1) : 123-141, March 2009

Effect of ergometric exercise on the activity in the serum of creatine phosphokinase, glutamate-oxaloacetate transminase and aldolase in rheumatoid arthritis.

SummaryAn ergometric exertion test was performed on 15 female rheumatoid arthritics with marked muscular atrophy, on 15 female arthritics without/with slight muscular atrophy and on 15 healthy female nurses.Minimal CPK activity appeared after exertion in 17 cases out of 30 among both rheumatoid groups, and in 6 out of 13 among the control group.Immediately following exertion there appeared a slight, but significant rise in GOT activity in both rheumatoid groups; in the control group the rise was insignificant.Aldolase activity remained at a significantly high level in both rheumatoid groups 60 minutes after exertion. In the control group the rise was insignificant.There were no significant differences between the female rheumatoid arthritics with marked muscular atrophy and without/with slight muscular atrophy with regard to urinary excretion of creatine and creatinine or to increase of serum GOT and aldolase activity after muscular exertion.

SGOT level in patients with muscular dystrophy.

Among the enzymes normally found in the body are the transaminases which catalyze the interconversion of an amino acid with a keto acid. The two most widely studied transaminases are glutamic oxaloacetic transminase and glutamic pyruvic transaminase, the former being the subject of the current study. Since skeletal muscle contains a high concentration of glutamic oxaloacetic transaminase, the present investigation, and extension of previous work with Fleisher, was carried out to ascertain whether the serum levels of this enzyme are altered in patients with muscular dystrophy. Historical Review Cohen studied these enzymes and, with Hekhuis, noted their wide distribution in the body. Another tissue enzyme, aldolase, was soon discovered by Warburg and Christian who found it to be elevated in the serum of rats with tumors. Sibley and Lehninger, in a survey of a large number of patients with various diseases, found that aldolase did not constitute a good test for cancer